| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-49534SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93829-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44049 | |||
| BSA Free | NBP2-61618-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87293-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74462 | |||
![Western Blot: PCK2 Antibody [NBP1-31241] - PCK2 antibody, Internal detects PCK2 protein. A. 30 ug HepG2 whole cell lysate/extract 7. 5 % SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NBP1-31241-img0005.jpg "Western Blot: PCK2 Antibody [NBP1-31241] - PCK2 antibody, Internal detects PCK2 protein. A. 30 ug HepG2 whole cell lysate/extract 7. 5 % SDS-PAGE gel, antibody dilution 1:1000.")
![Immunocytochemistry/Immunofluorescence: PCK2 Antibody [NBP1-31241] - Hep G2 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: PCK2 protein stained by PCK2 antibody [C1C2], Internal diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31241-img0006.jpg)
![Immunohistochemistry-Paraffin: PCK2 Antibody [NBP1-31241] - OVCAR3 xenograft, using PCK2 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP1-31241-img0002.jpg)
![Western Blot: PCK2 Antibody [NBP1-31241] - A. 30 ug Neuro2A whole cell lysate/extract, B. 30 ug GL261 whole cell lysate/extract 7. 5% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NBP1-31241-img0003.jpg)
![Western Blot: PCK2 Antibody [NBP1-31241] - 30 ug PC-12 whole cell lysate/extract 7. 5% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NBP1-31241-img0004.jpg)
![Western Blot: TIGAR/C12orf5 Antibody [NBP1-49534] - Analysis of TIGAR in HeLa whole cell extracts.](http://images.novusbio.com/fullsize/TIGAR-C12orf5-Antibody-Western-Blot-NBP1-49534-img0006.jpg "Western Blot: TIGAR/C12orf5 Antibody [NBP1-49534] - Analysis of TIGAR in HeLa whole cell extracts.")
![Immunohistochemistry: TIGAR/C12orf5 Antibody [NBP1-49534] - Analysis of TIGAR in mouse muscle.](http://images.novusbio.com/fullsize/TIGAR-C12orf5-Antibody-Immunohistochemistry-NBP1-49534-img0005.jpg)
![Western Blot: SREBP1 Antibody [NB100-74542] - Analysis of 25 ug of mouse liver (lane 1), rat liver (lane 2) and HepG2 (lane 3) cell lysates onto an SDS polyacrylamide gel.](http://images.novusbio.com/fullsize/SREBP1-Antibody-Western-Blot-NB100-74542-img0008.jpg "Western Blot: SREBP1 Antibody [NB100-74542] - Analysis of 25 ug of mouse liver (lane 1), rat liver (lane 2) and HepG2 (lane 3) cell lysates onto an SDS polyacrylamide gel.")
![Immunocytochemistry/Immunofluorescence: SREBP1 Antibody [NB100-74542] - Analysis of SREBP1 (green) showing staining in the cytoplasm of C2C12 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/SREBP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-74542-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: SREBP1 Antibody [NB100-74542] - Analysis of SREBP1 (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/SREBP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-74542-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: SREBP1 Antibody [NB100-74542] - Analysis of SREBP1 (green) showing staining in the cytoplasm of HepG2 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/SREBP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-74542-img0006.jpg)
![Western Blot: Glut4 Antibody [NBP1-49840] - Glucose Transporter GLUT4 expression in bovine skeletal muscle whole lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/Glut4-Antibody-Western-Blot-NBP1-49840-img0005.jpg "Western Blot: Glut4 Antibody [NBP1-49840] - Glucose Transporter GLUT4 expression in bovine skeletal muscle whole lysate. Image from verified customer review.")
![Immunohistochemistry-Paraffin: Glut4 Antibody [NBP1-49840] - 3.75ug/ml staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Glut4-Antibody-Immunohistochemistry-Paraffin-NBP1-49840-img0006.jpg)
![Western Blot: Glut4 Antibody [NBP1-49840] - Glucose Transporter GLUT4 in Mouse Heart lysate (35ug protein in RIPA buffer) using NBP1-49840 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Glut4-Antibody-Western-Blot-NBP1-49840-img0004.jpg)
![Western Blot: PDK4 Antibody [NBP2-93829] - Analysis of various lysates, using PDK4 antibody at 1:2350 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/PDK4-Antibody-Western-Blot-NBP2-93829-img0007.jpg "Western Blot: PDK4 Antibody [NBP2-93829] - Analysis of various lysates, using PDK4 antibody at 1:2350 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry-Paraffin: PDK4 Antibody [NBP2-93829] - Mouse heart using PDK4 Rabbit pAb (NBP2-93829) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PDK4-Antibody-Immunohistochemistry-Paraffin-NBP2-93829-img0006.jpg)
![Immunohistochemistry-Paraffin: PDK4 Antibody [NBP2-93829] - Rat heart using PDK4 Rabbit pAb (NBP2-93829) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PDK4-Antibody-Immunohistochemistry-Paraffin-NBP2-93829-img0004.jpg)
![Immunohistochemistry-Paraffin: PDK4 Antibody [NBP2-93829] - Human placenta using PDK4 Rabbit pAb (NBP2-93829) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PDK4-Antibody-Immunohistochemistry-Paraffin-NBP2-93829-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: PDK4 Antibody - Azide and BSA Free [PDK4] - Immunofluorescence analysis of U2OS cells using PDK4 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-93829_rabbit-polyclonal-pdk4-antibody-5220251638091.jpg)
![Western Blot: PGM1 Antibody (CL3299) [NBP2-61618] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/PGM1-Antibody-CL3299-Western-Blot-NBP2-61618-img0006.jpg "Western Blot: PGM1 Antibody (CL3299) [NBP2-61618] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line RT-4")
![Immunohistochemistry-Paraffin: PGM1 Antibody (CL3299) [NBP2-61618] - Staining of human fallopian tube shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/PGM1-Antibody-CL3299-Immunohistochemistry-Paraffin-NBP2-61618-img0005.jpg)
![Immunohistochemistry: PGM1 Antibody (CL3299) [NBP2-61618] - Staining of human skeletal muscle shows moderate cytoplasmic immunoreactivity in muscle fibers.](http://images.novusbio.com/fullsize/PGM1-Antibody-CL3299-Immunohistochemistry-NBP2-61618-img0001.jpg)
![Immunohistochemistry: PGM1 Antibody (CL3299) [NBP2-61618] - Staining of human liver shows strong positivity in hepatocytes.](http://images.novusbio.com/fullsize/PGM1-Antibody-CL3299-Immunohistochemistry-NBP2-61618-img0002.jpg)
![Immunohistochemistry: PGM1 Antibody (CL3299) [NBP2-61618] - Staining of human prostate shows moderate cytoplasmic immunoreactivity in smooth muscle.](http://images.novusbio.com/fullsize/PGM1-Antibody-CL3299-Immunohistochemistry-NBP2-61618-img0003.jpg)
![Immunohistochemistry: PGM1 Antibody (CL3299) [NBP2-61618] - Staining of human colon shows cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PGM1-Antibody-CL3299-Immunohistochemistry-NBP2-61618-img0004.jpg)
![Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NB100-1750] - Section of human colon adenocarcinoma. Antibody: Affinity purified rabbit PGC1 alpha Antibody used at a dilution of 1:250. Detection: DAB](http://images.novusbio.com/fullsize/PGC1-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-1750-img0006.jpg "Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NB100-1750] - Section of human colon adenocarcinoma. Antibody: Affinity purified rabbit PGC1 alpha Antibody used at a dilution of 1:250. Detection: DAB")
![Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NB100-1750] - Section of human stomach adenocarcinoma. Antibody: Affinity purified rabbit PGC1 alpha Antibody used at a dilution of 1:250. Detection: DAB](http://images.novusbio.com/fullsize/PGC1-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-1750-img0004.jpg)
![Western Blot: PCK1 Antibody (3E4) [H00005105-M01] - Analysis of PCK1 expression in transfected 293T cell line by PCK1 monoclonal antibody (M01), clone 3E4.Lane 1: PCK1 transfected lysate(69.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PCK1-Antibody-3E4-Western-Blot-H00005105-M01-img0008.jpg "Western Blot: PCK1 Antibody (3E4) [H00005105-M01] - Analysis of PCK1 expression in transfected 293T cell line by PCK1 monoclonal antibody (M01), clone 3E4.Lane 1: PCK1 transfected lysate(69.2 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: PCK1 Antibody (3E4) [H00005105-M01] - Analysis of monoclonal antibody to PCK1 on HepG2 cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PCK1-Antibody-3E4-Immunocytochemistry-Immunofluorescence-H00005105-M01-img0005.jpg)
![Western Blot: PCK1 Antibody (3E4) [H00005105-M01] - PCK1 monoclonal antibody (M01), clone 3E4 Analysis of PCK1 expression in HepG2.](http://images.novusbio.com/fullsize/PCK1-Antibody-3E4-Western-Blot-H00005105-M01-img0007.jpg)
![Immunoprecipitation: PCK1 Antibody (3E4) [H00005105-M01] - Analysis of PCK1 transfected lysate using anti-PCK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PCK1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/PCK1-Antibody-3E4-Immunoprecipitation-H00005105-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (HL2010) - Azide and BSA Free [NBP3-25599] - PFKM antibody [HL2010] detects PFKM protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. PFKM stained by PFKM antibody [HL2010] (NBP3-25599) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25599_rabbit-muscle-phosphofructokinase-pfkm-pfk-1-mab-hl2010-azide-bsa-free-2120249432858.jpg "Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (HL2010) - Azide and BSA Free [NBP3-25599] - PFKM antibody [HL2010] detects PFKM protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. PFKM stained by PFKM antibody [HL2010] (NBP3-25599) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (HL2010) - Azide and BSA Free [NBP3-25599] - PFKM antibody [HL2010] detects PFKM protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. PFKM stained by PFKM antibody [HL2010] (NBP3-25599) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25599_rabbit-muscle-phosphofructokinase-pfkm-pfk-1-mab-hl2010-azide-bsa-free-21202494149.jpg)
![Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (HL2010) - Azide and BSA Free [NBP3-25599] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PFKM antibody [HL2010] (NBP3-25599) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25599_rabbit-muscle-phosphofructokinase-pfkm-pfk-1-mab-hl2010-azide-bsa-free-212024941459.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (HL2010) - Azide and BSA Free [NBP3-25599] - Wild-type (WT) and PFKM knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PFKM antibody [HL2010] (NBP3-25599) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25599_rabbit-muscle-phosphofructokinase-pfkm-pfk-1-mab-hl2010-azide-bsa-free-2120249422638.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Muscle Phosphofructokinase/PFKM/PFK-1 antibody [HL2010] (NBP3-25599) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25599_rabbit-muscle-phosphofructokinase-pfkm-pfk-1-mab-hl2010-azide-bsa-free-western-blot-1411202518312123.jpg)
![Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Western-Blot-NBP1-87293-img0012.jpg "Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87293-img0013.jpg)
![Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87293-img0010.jpg)
![Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293] - Analysis in human skeletal muscle tissue.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Western-Blot-NBP1-87293-img0011.jpg)
![Western Blot: TBC1D4 Antibody (5E6) [NBP2-00597] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TBC1D4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TBC1D4.](http://images.novusbio.com/fullsize/TBC1D4-Antibody-5E6-Western-Blot-NBP2-00597-img0004.jpg "Western Blot: TBC1D4 Antibody (5E6) [NBP2-00597] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TBC1D4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TBC1D4.")
![Immunocytochemistry/Immunofluorescence: TBC1D4 Antibody (OTI5E6) [NBP2-00597] - TBC1D4 Antibody (5E6) [NBP2-00597] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TBC1D4.](http://images.novusbio.com/fullsize/TBC1D4-Antibody-5E6-Immunocytochemistry-Immunofluorescence-NBP2-00597-img0006.jpg)
![Flow Cytometry: TBC1D4 Antibody (5E6) [NBP2-00597] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TBC1D4 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/TBC1D4-Antibody-5E6-Flow-Cytometry-NBP2-00597-img0003.jpg)
![Flow Cytometry: TBC1D4 Antibody (5E6) [NBP2-00597] - Analysis of Hela cells, using anti-TBC1D4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/TBC1D4-Antibody-5E6-Flow-Cytometry-NBP2-00597-img0001.jpg)
![Flow Cytometry: TBC1D4 Antibody (5E6) [NBP2-00597] - Analysis of Jurkat cells, using anti-TBC1D4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/TBC1D4-Antibody-5E6-Flow-Cytometry-NBP2-00597-img0002.jpg)
![Western Blot: PCK2 Antibody (10L10J8) [NBP3-16418] - Western blot analysis of extracts of various cell lines, using PEPCK/PCK2 Rabbit mAb (NBP3-16418) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PCK2-Antibody-10L10J8-Western-Blot-NBP3-16418-img0002.jpg "Western Blot: PCK2 Antibody (10L10J8) [NBP3-16418] - Western blot analysis of extracts of various cell lines, using PEPCK/PCK2 Rabbit mAb (NBP3-16418) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: PCK2 Antibody (10L10J8) [NBP3-16418] - Immunofluorescence analysis of HeLa cells using PEPCK/PCK2 Rabbit mAb (NBP3-16418) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PCK2-Antibody-10L10J8-Immunocytochemistry-Immunofluorescence-NBP3-16418-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: PCK2 Antibody (10L10J8) [NBP3-16418] - Confocal imaging of C2C12 cells using [KO Validated] PCK2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16418_rabbit-pck2-mab-10l10j8-5220251242570.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PCK2 Antibody (10L10J8) [NBP3-16418] - Western blot analysis of lysates from wild type(WT) and PCK2 knockout (KO) 293T cells, using [KO Validated] PCK2 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-16418_rabbit-pck2-mab-10l10j8-522025124251.jpg)
![Immunocytochemistry/ Immunofluorescence: PCK2 Antibody (10L10J8) [NBP3-16418] - Confocal imaging of HeLa cells using [KO Validated] PCK2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16418_rabbit-pck2-mab-10l10j8-5220251255515.jpg)