| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54426-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43801 | |||
| BSA Free | NBP2-46626-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59619 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38698-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13893-25ul | |||
![Western Blot: SAT2 Antibody (OTI1C9) - Azide and BSA Free [NBP2-73990] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAT2.](http://images.novusbio.com/fullsize/SAT2-Antibody-OTI1C9-Azide-and-BSA-Free-Western-Blot-NBP2-73990-img0001.jpg "Western Blot: SAT2 Antibody (OTI1C9) - Azide and BSA Free [NBP2-73990] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAT2.")
![Immunocytochemistry/Immunofluorescence: SAT2 Antibody (OTI1C9) - Azide and BSA Free [NBP2-73990] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SAT2.](http://images.novusbio.com/fullsize/SAT2-Antibody-OTI1C9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-73990-img0004.jpg)
![Immunohistochemistry: SAT2 Antibody (OTI1C9) - Azide and BSA Free [NBP2-73990] - Staining of paraffin-embedded Human liver tissue using anti-SAT2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SAT2-Antibody-OTI1C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73990-img0010.jpg)
![Flow Cytometry: SAT2 Antibody (OTI1C9) - Azide and BSA Free [NBP2-73990] - Analysis of Jurkat cells, using anti-SAT2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/SAT2-Antibody-OTI1C9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73990-img0003.jpg)
![Immunohistochemistry: SAT2 Antibody (OTI1C9) - Azide and BSA Free [NBP2-73990] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SAT2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SAT2-Antibody-OTI1C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73990-img0005.jpg)
![Immunohistochemistry: SAT2 Antibody (OTI1C9) - Azide and BSA Free [NBP2-73990] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-SAT2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SAT2-Antibody-OTI1C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73990-img0006.jpg)
![Immunohistochemistry: SAT2 Antibody (OTI1C9) - Azide and BSA Free [NBP2-73990] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SAT2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SAT2-Antibody-OTI1C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73990-img0007.jpg)
![Immunohistochemistry: SAT2 Antibody (OTI1C9) - Azide and BSA Free [NBP2-73990] - Staining of paraffin-embedded Human bladder tissue using anti-SAT2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SAT2-Antibody-OTI1C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73990-img0008.jpg)
![Immunohistochemistry: SAT2 Antibody (OTI1C9) - Azide and BSA Free [NBP2-73990] - Staining of paraffin-embedded Human Kidney tissue using anti-SAT2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SAT2-Antibody-OTI1C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73990-img0009.jpg)
![Immunohistochemistry: SAT2 Antibody (OTI1C9) - Azide and BSA Free [NBP2-73990] - Staining of paraffin-embedded Human pancreas tissue using anti-SAT2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SAT2-Antibody-OTI1C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73990-img0011.jpg)
![Flow Cytometry: SAT2 Antibody (OTI1C9) - Azide and BSA Free [NBP2-73990] - Analysis of Hela cells, using anti-SAT2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/SAT2-Antibody-OTI1C9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73990-img0002.jpg)
![Western Blot: S100B Antibody (SC57-02) [NBP2-67572] - Analysis of S100 beta on mouse liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/S100B-Antibody-SC57-02-Western-Blot-NBP2-67572-img0007.jpg "Western Blot: S100B Antibody (SC57-02) [NBP2-67572] - Analysis of S100 beta on mouse liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: S100B Antibody (SC57-02) [NBP2-67572] - Staining S100 beta in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S100B-Antibody-SC57-02-Immunocytochemistry-Immunofluorescence-NBP2-67572-img0005.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (SC57-02) [NBP2-67572] - Analysis of paraffin-embedded mouse brain tissue using anti-S100 beta antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S100B-Antibody-SC57-02-Immunohistochemistry-Paraffin-NBP2-67572-img0003.jpg)
![Western Blot: S100B Antibody (SC57-02) [NBP2-67572] - Analysis of S100 beta on zebrafish tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/S100B-Antibody-SC57-02-Western-Blot-NBP2-67572-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (SC57-02) [NBP2-67572] - Staining S100 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S100B-Antibody-SC57-02-Immunocytochemistry-Immunofluorescence-NBP2-67572-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (SC57-02) [NBP2-67572] - Staining S100 beta in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S100B-Antibody-SC57-02-Immunocytochemistry-Immunofluorescence-NBP2-67572-img0002.jpg)
![Western Blot: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653] - Western blot analysis of RXR alpha/NR2B1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: MCF-7 cell lysate](http://images.novusbio.com/fullsize/RXR-alpha-NR2B1-Antibody-JG99-38-Western-Blot-NBP2-75653-img0006.jpg "Western Blot: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653] - Western blot analysis of RXR alpha/NR2B1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: MCF-7 cell lysate")
![Immunocytochemistry/Immunofluorescence: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653] - Staining Retinoid X Receptor alpha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/RXR-alpha-NR2B1-Antibody-JG99-38-Immunocytochemistry-Immunofluorescence-NBP2-75653-img0002.jpg)
![Immunohistochemistry-Paraffin: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653] - Analysis of paraffin-embedded rat liver tissue using anti-Retinoid X Receptor alpha antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/RXR-alpha-NR2B1-Antibody-JG99-38-Immunohistochemistry-Paraffin-NBP2-75653-img0004.jpg)
![Western Blot: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653] - Analysis of Retinoid X Receptor alpha on MCF-7 cell using anti-Retinoid X Receptor alpha antibody at 1/5,000 dilution.](http://images.novusbio.com/fullsize/RXR-alpha-NR2B1-Antibody-JG99-38-Western-Blot-NBP2-75653-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653] - Staining Retinoid X Receptor alpha in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/RXR-alpha-NR2B1-Antibody-JG99-38-Immunocytochemistry-Immunofluorescence-NBP2-75653-img0001.jpg)
![Immunohistochemistry-Paraffin: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653] - Analysis of paraffin-embedded human esophagus tissue using anti-Retinoid X Receptor alpha antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/RXR-alpha-NR2B1-Antibody-JG99-38-Immunohistochemistry-Paraffin-NBP2-75653-img0003.jpg)
![Western Blot: 14-3-3 epsilon Antibody [NBP1-32695] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 epsilon antibody [N1C3] diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Western-Blot-NBP1-32695-img0017.jpg "Western Blot: 14-3-3 epsilon Antibody [NBP1-32695] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 epsilon antibody [N1C3] diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: 14-3-3 epsilon Antibody [NBP1-32695] - Antibody was tested at 1:200 in HeLa cells with FITC (green). Nuclei were counterstained with Dapi (blue).](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32695-img0001.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody [NBP1-32695] - A549 xenograft , using 14-3-3 epsilon antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Immunohistochemistry-Paraffin-NBP1-32695-img0013.jpg)
![Western Blot: 14-3-3 epsilon Antibody [NBP1-32695] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 epsilon antibody [N1C3] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Western-Blot-NBP1-32695-img0015.jpg)
![Immunoprecipitation: 14-3-3 epsilon Antibody [NBP1-32695] - IP samples: HeLa whole cell extract A. 40 ug HeLa whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of YWHAE protein by 4 ug 14-3-3 epsilon antibody [N1C3] (NBP1-32695) 12 % SDS-PAGE The immunoprecipitated YWHAE protein was detected by 14-3-3 epsilon antibody [N1C3] (NBP1-32695) diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Immunoprecipitation-NBP1-32695-img0012.jpg)
![Western Blot: S100B Antibody (S100B/1706R) [NBP2-53188] - Western blot analysis of human brain tissue lysate using S100B antibody (S100B/1706R).](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1706R-Western-Blot-NBP2-53188-img0003.jpg "Western Blot: S100B Antibody (S100B/1706R) [NBP2-53188] - Western blot analysis of human brain tissue lysate using S100B antibody (S100B/1706R).")
![Immunohistochemistry-Paraffin: S100B Antibody (S100B/1706R) [NBP2-53188] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100B Rabbit Monoclonal Antibody (S100B/1706R).](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1706R-Immunohistochemistry-Paraffin-NBP2-53188-img0001.jpg)
![Protein Array: S100B Antibody (S100B/1706R) [NBP2-53188] - Analysis of Protein Array containing more than 19,000 full-length human proteins using S100B Antibody (S100B/1706R) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1706R-Protein-Array-NBP2-53188-img0002.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (CL2720) [NBP2-46626] - Staining in human cerebral cortex and kidney tissues. Corresponding S100B RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/S100B-Antibody-CL2720-Immunohistochemistry-Paraffin-NBP2-46626-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: S100B Antibody (CL2720) [NBP2-46626] - Staining in human cerebral cortex and kidney tissues. Corresponding S100B RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: S100B Antibody (CL2720) [NBP2-46626] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/S100B-Antibody-CL2720-Western-Blot-NBP2-46626-img0007.jpg)
![Immunohistochemistry: S100B Antibody (CL2720) [NBP2-46626] - Staining of rat brain shows moderate positivity in astrocytes in the hippocampus.](http://images.novusbio.com/fullsize/S100B-Antibody-CL2720-Immunohistochemistry-Paraffin-NBP2-46626-img0016.jpg)
![Immunohistochemistry: S100B Antibody (CL2720) [NBP2-46626] - Staining of mouse brain shows moderate positivity in the cerebellum.](http://images.novusbio.com/fullsize/S100B-Antibody-CL2720-Immunohistochemistry-Paraffin-NBP2-46626-img0008.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (CL2720) [NBP2-46626] - Staining of human cerebral cortex shows moderate nuclear positivity in astrocytes.](http://images.novusbio.com/fullsize/S100B-Antibody-CL2720-Immunohistochemistry-Paraffin-NBP2-46626-img0009.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (CL2720) [NBP2-46626] - Staining of human cerebellum shows strong nuclear positivity in glial cells.](http://images.novusbio.com/fullsize/S100B-Antibody-CL2720-Immunohistochemistry-Paraffin-NBP2-46626-img0010.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (CL2720) [NBP2-46626] - Staining of human kidney shows no nuclear positivity as expected.](http://images.novusbio.com/fullsize/S100B-Antibody-CL2720-Immunohistochemistry-Paraffin-NBP2-46626-img0011.jpg)
![Immunohistochemistry: S100B Antibody (CL2720) [NBP2-46626] - Staining of rat brain shows strong positivity in glial cells in the cerebellum.](http://images.novusbio.com/fullsize/S100B-Antibody-CL2720-Immunohistochemistry-Paraffin-NBP2-46626-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (S100B/1012) [NBP2-59618] - Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue). (Negative Control)](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1012-Immunocytochemistry-Immunofluorescence-NBP2-59618-img0007.jpg "Immunocytochemistry/Immunofluorescence: S100B Antibody (S100B/1012) [NBP2-59618] - Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue). (Negative Control)")
![Protein Array: S100B Antibody (S100B/1012) [NBP2-59618] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1012-Protein-Array-NBP2-59618-img0001.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (S100B/1012) [NBP2-59618] - Formalin-fixed. Paraffin-embedded human Melanoma stained with S100B Mouse Monoclonal Antibody (S100B/1012).](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1012-Immunohistochemistry-Paraffin-NBP2-59618-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (S100B/1012) [NBP2-59618] - Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled S100B Antibody (S100B/1012) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1012-Immunocytochemistry-Immunofluorescence-NBP2-59618-img0006.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (S100B/1012) [NBP2-59618] - Formalin-fixed, paraffin-embedded human Schwanoma stained with S100B Mouse Monoclonal Antibody (S100B/1012).](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1012-Immunohistochemistry-Paraffin-NBP2-59618-img0004.jpg)
![Western Blot: Carbonic Anhydrase III/CA3 Antibody (4A12-1A3) [H00000761-M02] - CA3 monoclonal antibody (M02), clone 4A12-1A3 Analysis of CA3 expression in K-562.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-III-CA3-Antibody-4A12-1A3-Western-Blot-H00000761-M02-img0010.jpg "Western Blot: Carbonic Anhydrase III/CA3 Antibody (4A12-1A3) [H00000761-M02] - CA3 monoclonal antibody (M02), clone 4A12-1A3 Analysis of CA3 expression in K-562.")
![Immunohistochemistry-Paraffin: Carbonic Anhydrase III/CA3 Antibody (4A12-1A3) [H00000761-M02] - Analysis of monoclonal antibody to CA3 on formalin-fixed paraffin-embedded human skeletal muscle. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-III-CA3-Antibody-4A12-1A3-Immunohistochemistry-Paraffin-H00000761-M02-img0008.jpg)
![Sandwich ELISA: Carbonic Anhydrase III/CA3 Antibody (4A12-1A3) [H00000761-M02] - Detection limit for recombinant GST tagged CA3 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-III-CA3-Antibody-4A12-1A3-Sandwich-ELISA-H00000761-M02-img0009.jpg)
![Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of Tryptophan Hydroxylase 1/TPH1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HL-60 cell lysate Lane 2: Hela cell lysate](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-SC53-07-Western-Blot-NBP2-67580-img0004.jpg "Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of Tryptophan Hydroxylase 1/TPH1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HL-60 cell lysate Lane 2: Hela cell lysate")
![Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of paraffin-embedded rat brain tissue using anti-Tryptophan Hydroxylase/TPH-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-SC53-07-Immunohistochemistry-Paraffin-NBP2-67580-img0005.jpg)
![Flow Cytometry: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of Hela cells with TPH1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-SC53-07-Flow-Cytometry-NBP2-67580-img0001.jpg)
![Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of paraffin-embedded mouse cerebellum tissue using anti-TPH1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-SC53-07-Immunohistochemistry-Paraffin-NBP2-67580-img0002.jpg)
![Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of paraffin-embedded rat brain tissue using anti-TPH1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-SC53-07-Immunohistochemistry-Paraffin-NBP2-67580-img0003.jpg)
![Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of TPH1 on different lysates with Rabbit anti-TPH1 antibody at 1/1,000 dilution.Lane 1: MDA-MB-231-si NT cell lysateLane 2: MDA-MB-231-si TPH1 cell lysateLysates/proteins at 10 ug/Lane.Predicted band size: 51 kDaObserved band size: 72 kDaExposure time: 3 minutes;4-20% SDS-PAGE gel.NBP2-67580 was shown to specifically react with TPH1 in MDA-MB-231-si NT cells. Weakened band was observed when MDA-MB-231-si TPH1 sample was tested. MDA-MB-231-si NT and Hela-si TPH1 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1/1,000) and Loading control antibody (Rabbit anti-GAPDH, (1/10,000) were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-rabbit IgG-HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67580_rabbit-monoclonal-tryptophan-hydroxylase-1-tph-1-antibody-sc53-07-western-blot-462024131133..jpg)
![Western Blot: ARH Antibody (4G4-D5) [H00026119-M01] - LDLRAP1 monoclonal antibody (M01), clone 4G4-D5 Analysis of LDLRAP1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/ARH-Antibody-4G4-D5-Western-Blot-H00026119-M01-img0010.jpg "Western Blot: ARH Antibody (4G4-D5) [H00026119-M01] - LDLRAP1 monoclonal antibody (M01), clone 4G4-D5 Analysis of LDLRAP1 expression in Hela S3 NE.")
![Immunocytochemistry/Immunofluorescence: ARH Antibody (4G4-D5) [H00026119-M01] - Analysis of monoclonal antibody to LDLRAP1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/ARH-Antibody-4G4-D5-Immunocytochemistry-Immunofluorescence-H00026119-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: ARH Antibody (4G4-D5) [H00026119-M01] - Analysis of monoclonal antibody to LDLRAP1 on formalin-fixed paraffin-embedded human maligant fibrous histiocytoma tissue. Antibody concentration 2 ug/ml.](http://images.novusbio.com/fullsize/ARH-Antibody-4G4-D5-Immunohistochemistry-Paraffin-H00026119-M01-img0008.jpg)
![Western Blot: ARH Antibody (4G4-D5) [H00026119-M01] - Analysis of LDLRAP1 expression in transfected 293T cell line by LDLRAP1 monoclonal antibody (M01), clone 4G4-D5.Lane 1: LDLRAP1 transfected lysate(29 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ARH-Antibody-4G4-D5-Western-Blot-H00026119-M01-img0011.jpg)
![Sandwich ELISA: ARH Antibody (4G4-D5) [H00026119-M01] - Detection limit for recombinant GST tagged LDLRAP1 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ARH-Antibody-4G4-D5-Sandwich-ELISA-H00026119-M01-img0009.jpg)
![Immunohistochemistry-Paraffin: CYP21A2 Antibody [NBP2-38698] - Analysis in human adrenal gland and kidney tissues using NBP2-38698 antibody. Corresponding CYP21A2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CYP21A2-Antibody-Immunohistochemistry-Paraffin-NBP2-38698-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CYP21A2 Antibody [NBP2-38698] - Analysis in human adrenal gland and kidney tissues using NBP2-38698 antibody. Corresponding CYP21A2 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: CYP21A2 Antibody [NBP2-38698] - Staining of human adrenal gland, kidney, pancreas and testis using Anti-CYP21A2 antibody NBP2-38698 (A) shows similar protein distribution across tissues to independent antibody NBP2-13893 (B).](http://images.novusbio.com/fullsize/CYP21A2-Antibody-Immunohistochemistry-Paraffin-NBP2-38698-img0013.jpg)
![Western Blot: CYP21A2 Antibody [NBP2-38698] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Adrenal Gland tissue](http://images.novusbio.com/fullsize/CYP21A2-Antibody-Western-Blot-NBP2-38698-img0002.jpg)
![Immunohistochemistry-Paraffin: CYP21A2 Antibody [NBP2-38698] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/CYP21A2-Antibody-Immunohistochemistry-Paraffin-NBP2-38698-img0017.jpg)
![Immunohistochemistry-Paraffin: CYP21A2 Antibody [NBP2-38698] - Staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells in adrenal cortex.](http://images.novusbio.com/fullsize/CYP21A2-Antibody-Immunohistochemistry-Paraffin-NBP2-38698-img0015.jpg)
![Immunohistochemistry-Paraffin: CYP21A2 Antibody [NBP2-38698] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/CYP21A2-Antibody-Immunohistochemistry-Paraffin-NBP2-38698-img0016.jpg)
![Immunohistochemistry-Paraffin: CYP21A2 Antibody [NBP2-38698] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/CYP21A2-Antibody-Immunohistochemistry-Paraffin-NBP2-38698-img0018.jpg)
![Western Blot: CYP21A2 Antibody [NBP2-13893] - Analysis in human adrenal gland tissue.](http://images.novusbio.com/fullsize/CYP21A2-Antibody-Western-Blot-NBP2-13893-img0005.jpg "Western Blot: CYP21A2 Antibody [NBP2-13893] - Analysis in human adrenal gland tissue.")
![Immunohistochemistry-Paraffin: CYP21A2 Antibody [NBP2-13893] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/CYP21A2-Antibody-Immunohistochemistry-Paraffin-NBP2-13893-img0020.jpg)
![Immunohistochemistry-Paraffin: CYP21A2 Antibody [NBP2-13893] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/CYP21A2-Antibody-Immunohistochemistry-Paraffin-NBP2-13893-img0012.jpg)
![Immunohistochemistry-Paraffin: CYP21A2 Antibody [NBP2-13893] - Staining of human testis.](http://images.novusbio.com/fullsize/CYP21A2-Antibody-Immunohistochemistry-Paraffin-NBP2-13893-img0013.jpg)
![Immunohistochemistry-Paraffin: CYP21A2 Antibody [NBP2-13893] - Staining of human kidney.](http://images.novusbio.com/fullsize/CYP21A2-Antibody-Immunohistochemistry-Paraffin-NBP2-13893-img0014.jpg)
![Immunohistochemistry-Paraffin: CYP21A2 Antibody [NBP2-13893] - Staining of human adrenal gland using Anti-CYP21A2 antibody NBP2-13893.](http://images.novusbio.com/fullsize/CYP21A2-Antibody-Immunohistochemistry-Paraffin-NBP2-13893-img0015.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: CYP21A2 Antibody [NBP2-13893] - Staining of human adrenal gland, kidney, pancreas and testis using Anti-CYP21A2 antibody NBP2-13893 (A) shows similar protein distribution across tissues to independent antibody NBP2-38698 (B).](http://images.novusbio.com/fullsize/CYP21A2-Antibody-Immunohistochemistry-Paraffin-NBP2-13893-img0016.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CYP21A2 Antibody [NBP2-13893] - Analysis in human adrenal gland and kidney tissues using NBP2-13893 antibody. Corresponding CYP21A2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CYP21A2-Antibody-Immunohistochemistry-Paraffin-NBP2-13893-img0017.jpg)
![Immunohistochemistry-Paraffin: CYP21A2 Antibody [NBP2-13893] - Staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells in adrenal cortex.](http://images.novusbio.com/fullsize/CYP21A2-Antibody-Immunohistochemistry-Paraffin-NBP2-13893-img0018.jpg)
![Immunohistochemistry-Paraffin: CYP21A2 Antibody [NBP2-13893] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/CYP21A2-Antibody-Immunohistochemistry-Paraffin-NBP2-13893-img0019.jpg)
![Immunohistochemistry-Paraffin: CYP21A2 Antibody [NBP2-13893] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/CYP21A2-Antibody-Immunohistochemistry-Paraffin-NBP2-13893-img0021.jpg)