| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76810SS | |||
| BSA Free | NBP2-76810 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43962 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76810SS | |||
| Azide and BSA Free | NBP2-80713 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75977SS | |||
| Azide and BSA Free | NBP2-80764 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33152 | |||
| IHC-Prediluted | NBP2-48405 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-74679SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43815 | |||
| BSA Free | NBP2-46629-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75787 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43961 | |||
| BSA Free | NBP2-59777-25ul | |||
![Western Blot: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - Total protein from human HeLa, THP-1 and U87 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-PHD2 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Western-Blot-NBP2-80713-img0001.jpg "Western Blot: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - Total protein from human HeLa, THP-1 and U87 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-PHD2 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - EGLN1/PHD2 was detected in immersion fixed WM-115 human malignant melanoma cell line using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog #NBP2-76810) at 3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm and nuclei. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80713-img0002.jpg)
![Immunohistochemistry-Paraffin: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - EGLN1/PHD2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-76810) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte(TM).](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80713-img0003.jpg)
![Flow Cytometry: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - Human Jurkat T Cell Leukemia Cell Line was stained with Rabbit Anti- EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-76810, filled histogram) or Rabbit IgG Isotype Control Antibody (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80713-img0004.jpg)
![Western Blot: COX4 Antibody (CL3497) [NBP2-78718] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2, mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3497-Western-Blot-NBP2-78718-img0002.jpg "Western Blot: COX4 Antibody (CL3497) [NBP2-78718] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2, mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunohistochemistry-Paraffin: COX4 Antibody (CL3497) [NBP2-78718] - Staining of human prostate shows strong cytoplasmic immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3497-Immunohistochemistry-Paraffin-NBP2-78718-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: COX4 Antibody (CL3497) [NBP2-78718] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-COX4I1 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3497-Western-Blot-NBP2-78718-img0001.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (CL3497) [NBP2-78718] - Staining of human fallopian tube shows strong cytoplasmic positivity in epithelial cells.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3497-Immunohistochemistry-Paraffin-NBP2-78718-img0003.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (CL3497) [NBP2-78718] - Staining of human stomach shows strong cytoplasmic immunoreactivity in glandular epithelium cells.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3497-Immunohistochemistry-Paraffin-NBP2-78718-img0004.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (CL3497) [NBP2-78718] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3497-Immunohistochemistry-Paraffin-NBP2-78718-img0005.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (CL3497) [NBP2-78718] - Staining of human kidney shows strong cytoplasmic positivity in renal tubules and glomerulus cells.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3497-Immunohistochemistry-Paraffin-NBP2-78718-img0006.jpg)
![Western Blot: COX4 Antibody [NBP2-15975] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/COX4-Antibody-Western-Blot-NBP2-15975-img0015.jpg "Western Blot: COX4 Antibody [NBP2-15975] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: COX4 Antibody [NBP2-15975] - COX4 antibody detects COX4 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: COX4 stained by COX4 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/COX4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15975-img0014.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody [NBP2-15975] - COX4 antibody detects COX4 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. COX4 stained by COX4 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/COX4-Antibody-Immunohistochemistry-Paraffin-NBP2-15975-img0016.jpg)
![Western Blot: COX4 Antibody [NBP2-15975] - Sample (50 ug of whole cell lysate) A: rat muscle, 12% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/COX-IV-isoform-1-Antibody-Western-Blot-NBP2-15975-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: COX4 Antibody [NBP2-15975] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/COX4-Antibody-Western-Blot-NBP2-15975-img0006.jpg)
![Western Blot: COX4 Antibody [NBP2-15975] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/COX4-Antibody-Western-Blot-NBP2-15975-img0013.jpg)
![Western Blot: COX4 Antibody [NBP2-15975] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (NBP2-15975) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15975_rabbit-polyclonal-cox4-antibody-9102024204120.jpg)
![Immunocytochemistry/ Immunofluorescence: COX4 Antibody [NBP2-15975] - COX4 antibody detects COX4 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: COX4 stained by COX4 antibody (NBP2-15975) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp2-15975_rabbit-polyclonal-cox4-antibody-91020242063363.jpg)
![Immunohistochemistry-Paraffin: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - EGLN1/PHD2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog #NBP2-76810) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyt(TM) HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell cytoplasm and nuclei.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Immunohistochemistry-Paraffin-NBP2-76810-img0003.jpg "Immunohistochemistry-Paraffin: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - EGLN1/PHD2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog #NBP2-76810) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyt(TM) HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell cytoplasm and nuclei.")
![Western Blot: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - Total protein from human HeLa, THP-1 and U87 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-PHD2 antibody in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Western-Blot-NBP2-76810-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - EGLN1/PHD2 was detected in immersion fixed WM-115 human malignant melanoma cell line using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-76810) at 3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLight(TM) 557-conjugated Anti-Rabbit IgG secondary antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm and nuclei.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Immunocytochemistry-Immunofluorescence-NBP2-76810-img0002.jpg)
![Flow Cytometry: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - Human Jurkat T Cell Leukemia Cell Line was stained with Rabbit anti-EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-76810, filled histogram) or Rabbit IgG Isotype Control Antibody (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Flow-Cytometry-NBP2-76810-img0001.jpg)
![Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Western-Blot-NBP2-75977-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (NBP2-75977) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at an approximatel molecular weight of 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control.This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Knockdown-Validated-NBP2-75977-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Immunocytochemistry-Immunofluorescence-NBP2-75977-img0001.jpg)
![Simple Western: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20ug/ml. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Simple-Western-NBP2-75977-img0004.jpg)
![Simple Western: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Simple Western lane view shows lysates of MCF +/- DMOG, BioSpherix MSCs in hypoxic conditions, and BioSpherix MSCs in normoxic conditions loaded at 0.5 mg/ml. A band was detected for HIF-1 alpha at approximately 116 kDa (as indicated) using NBP2-75977 (1:100 dilution) followed by Anti-Rabbit Secondary Antibody (042-206, ProteinSimple). This experiment was conducted under standard assay conditions, and using the 12-230 kDa separation module (SW-W004). Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Simple-Western-NBP2-75977-img0005.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (66.4.C2 (PN-15)) [NBP2-15199] - FFPE human renal cell carcinoma stained with RCC Ab (66.4.C2). Note cytoplasmic & cell surface staining of tumor cells.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IX-CA9-Antibody-66-4-C2-PN-15-Immunohistochemistry-Paraffin-NBP2-15199-img0006.jpg "Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (66.4.C2 (PN-15)) [NBP2-15199] - FFPE human renal cell carcinoma stained with RCC Ab (66.4.C2). Note cytoplasmic & cell surface staining of tumor cells.")
![Western Blot: HIF-1 alpha [Hydroxy Pro564] Antibody [NB110-74679] - Detection (left panel) of HIF-1 alpha [Hydroxy Pro564] in nuclear extracts of A549 cells treated with MG132 (a proteasome inhibitor). Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining is shown (right panel) using conventional anti-HIF-1alpha. The asterisk marks a band approximately 110 kDa molecular weight in size corresponding to HIF-1 alpha. The primary antibody was used at a 1:1000 dilution in 2% BLOTTO.](http://images.novusbio.com/fullsize/HIF-1-alpha-[Hydroxy-Pro564]-Antibody-Western-Blot-NB110-74679-img0004.jpg "Western Blot: HIF-1 alpha [Hydroxy Pro564] Antibody [NB110-74679] - Detection (left panel) of HIF-1 alpha [Hydroxy Pro564] in nuclear extracts of A549 cells treated with MG132 (a proteasome inhibitor). Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining is shown (right panel) using conventional anti-HIF-1alpha. The asterisk marks a band approximately 110 kDa molecular weight in size corresponding to HIF-1 alpha. The primary antibody was used at a 1:1000 dilution in 2% BLOTTO.")
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Analysis in human cerebral cortex and tonsil tissues using NBP2-46629 antibody. Corresponding GAD2/GAD65 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-Paraffin-NBP2-46629-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Analysis in human cerebral cortex and tonsil tissues using NBP2-46629 antibody. Corresponding GAD2/GAD65 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Western-Blot-NBP2-46629-img0009.jpg)
![Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of mouse midbrain shows moderate positivity in GABergic neural fibers in the substantia nigra.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-NBP2-46629-img0014.jpg)
![Western Blot: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Western-Blot-NBP2-46629-img0008.jpg)
![Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of rat basal forebrain shows moderate immunoreactivity in GABAergic fibers.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-NBP2-46629-img0001.jpg)
![Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of mouse substantia nigra shows moderate positivity in GABAergic fibers in pars reticulata.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-NBP2-46629-img0002.jpg)
![Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of rat globus pallidus shows moderate positivity in GABAergic neural fibers.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-NBP2-46629-img0004.jpg)
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of human cerebellum shows moderate immunoreactivity in GABAergic fibers in granular and molecular layers.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-Paraffin-NBP2-46629-img0005.jpg)
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of human cerebral cortex shows moderate to strong positivity in GABAergic neuronal processes.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-Paraffin-NBP2-46629-img0011.jpg)
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of human pancreas shows moderate cytoplasmic positivity in endocrine glandular cells.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-Paraffin-NBP2-46629-img0012.jpg)
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-Paraffin-NBP2-46629-img0013.jpg)
![Western Blot: Glut1 Antibody (GLUT1/2476) [NBP2-75785] - Western Blot Analysis of Human A431 cell lysate using Glut1 Antibody (GLUT1/2476).](http://images.novusbio.com/fullsize/Glut1-Antibody-GLUT1-2476-Western-Blot-NBP2-75785-img0007.jpg "Western Blot: Glut1 Antibody (GLUT1/2476) [NBP2-75785] - Western Blot Analysis of Human A431 cell lysate using Glut1 Antibody (GLUT1/2476).")
![Immunocytochemistry/Immunofluorescence: Glut1 Antibody (GLUT1/2476) [NBP2-75785] - Immunofluorescence staining of MCF-7 cells using Glut1 Antibody (GLUT1/2476) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.](http://images.novusbio.com/fullsize/Glut1-Antibody-GLUT1-2476-Immunocytochemistry-Immunofluorescence-NBP2-75785-img0005.jpg)
![Immunohistochemistry-Paraffin: Glut1 Antibody (GLUT1/2476) [NBP2-75785] - Immunohistochemical analysis of formalin-fixed, paraffin-embedded human bladder stained with GLUT-1 Mouse Monoclonal Antibody (GLUT1/2476).](http://images.novusbio.com/fullsize/Glut1-Antibody-GLUT1-2476-Immunohistochemistry-Paraffin-NBP2-75785-img0002.jpg)
![Flow Cytometry: Glut1 Antibody (GLUT1/2476) [NBP2-75785] - Flow Cytometric Analysis of K562 cells using Glut1 Antibody (GLUT1/2476) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Glut1-Antibody-GLUT1-2476-Flow-Cytometry-NBP2-75785-img0004.jpg)
![Immunohistochemistry-Paraffin: Glut1 Antibody (GLUT1/2476) [NBP2-75785] - Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma stained with GLUT-1 Mouse Monoclonal Antibody (GLUT1/2476).](http://images.novusbio.com/fullsize/Glut1-Antibody-GLUT1-2476-Immunohistochemistry-Paraffin-NBP2-75785-img0001.jpg)
![Protein Array: Glut1 Antibody (GLUT1/2476) [NBP2-75785] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Glut1-Antibody-GLUT1-2476-Protein-Array-NBP2-75785-img0006.jpg)
![Western Blot: GAD2/GAD65 Antibody [NBP1-33284] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD65 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-Western-Blot-NBP1-33284-img0012.jpg "Western Blot: GAD2/GAD65 Antibody [NBP1-33284] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD65 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NBP1-33284] - RT2 xenograft. GAD65 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-Immunohistochemistry-Paraffin-NBP1-33284-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: GAD2/GAD65 Antibody [NBP1-33284] - Paraformaldehyde-fixed HeLa, using GAD65 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with Red at 1:2500.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33284-img0008.jpg)
![GAD65 antibody detects GAD65 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GAD65 stained by GAD65 antibody (NBP1-33284) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-33284_rabbit-polyclonal-gad2-gad65-antibody-immunocytochemistry-immunofluorescence-2611202518365910.jpg)
![Western Blot: COX4 Antibody (1327) [NBP2-59777] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-COX4I1 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.](http://images.novusbio.com/fullsize/COX4-Antibody-1327-Western-Blot-NBP2-59777-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: COX4 Antibody (1327) [NBP2-59777] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-COX4I1 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: COX4 Antibody (1327) [NBP2-59777] - Staining of human liver shows cytoplasmic immunoreactivity in hepatocytes.](http://images.novusbio.com/fullsize/COX4-Antibody-1327-Immunohistochemistry-Paraffin-NBP2-59777-img0005.jpg)
![Western Blot: COX4 Antibody (1327) [NBP2-59777] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2 and mouse cell line NIH-3T3.](http://images.novusbio.com/fullsize/COX4-Antibody-1327-Western-Blot-NBP2-59777-img0006.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (1327) [NBP2-59777] - Staining of human fallopian tube shows strong cytoplasmic immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/COX4-Antibody-1327-Immunohistochemistry-Paraffin-NBP2-59777-img0001.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (1327) [NBP2-59777] - Staining of human kidney shows strong cytoplasmic positivity in renal tubules cells.](http://images.novusbio.com/fullsize/COX4-Antibody-1327-Immunohistochemistry-Paraffin-NBP2-59777-img0002.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (1327) [NBP2-59777] - Staining of human stomach shows strong cytoplasmic immunoreactivity in glandular epithelium cells.](http://images.novusbio.com/fullsize/COX4-Antibody-1327-Immunohistochemistry-Paraffin-NBP2-59777-img0003.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (1327) [NBP2-59777] - Staining of human prostate shows cytoplasmic positivity in the epithelial cells.](http://images.novusbio.com/fullsize/COX4-Antibody-1327-Immunohistochemistry-Paraffin-NBP2-59777-img0004.jpg)
![IHC-P- Glut1 Antibody (10O1I5) [NBP3-15433] - Analysis of GLUT1/SLC2A1 in paraffin-embedded human cervix cancer tissue using GLUT1/SLC2A1 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15433_rabbit-glut1-mab-10o1i5-immunohistochemistry-paraffin-2512024131615..jpg "IHC-P- Glut1 Antibody (10O1I5) [NBP3-15433] - Analysis of GLUT1/SLC2A1 in paraffin-embedded human cervix cancer tissue using GLUT1/SLC2A1 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.")
![IHC-P- Glut1 Antibody (10O1I5) [NBP3-15433] - Analysis of GLUT1/SLC2A1 in paraffin-embedded human liver tissue using GLUT1/SLC2A1 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15433_rabbit-glut1-mab-10o1i5-immunohistochemistry-paraffin-251202413183..jpg)
![IHC-P- Glut1 Antibody (10O1I5) [NBP3-15433] - Mouse liver tissue using GLUT1/SLC2A1 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15433_rabbit-glut1-mab-10o1i5-immunohistochemistry-paraffin-2512024132028..jpg)
![IHC-P- Glut1 Antibody (10O1I5) [NBP3-15433] -Human pancreas tissue using GLUT1/SLC2A1 Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15433_rabbit-glut1-mab-10o1i5-immunohistochemistry-paraffin-126202493951..jpg)
![IHC-P- Glut1 Antibody (10O1I5) [NBP3-15433] -Human placenta tissue using GLUT1/SLC2A1 Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15433_rabbit-glut1-mab-10o1i5-immunohistochemistry-paraffin-126202494056..jpg)
![Immunohistochemistry: Glut1 Antibody (10O1I5) [Glut1] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Glut1 Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15433_rabbit-glut1-mab-10o1i5-52202517152749.jpg)
![Immunohistochemistry: Glut1 Antibody (10O1I5) [Glut1] - Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue using Glut1 Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15433_rabbit-glut1-mab-10o1i5-52202517273598.jpg)
