| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85366-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80722-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70830 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70329 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88054-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88055-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84556-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81840-25ul | |||
![Western Blot: Cytochrome P450 2E1 Antibody [NBP1-85366] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue](http://images.novusbio.com/fullsize/Cytochrome-P450-2E1-Antibody-Western-Blot-NBP1-85366-img0004.jpg "Western Blot: Cytochrome P450 2E1 Antibody [NBP1-85366] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue")
![Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366] - Staining of human liver shows strong cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/Cytochrome-P450-2E1-Antibody-Immunohistochemistry-Paraffin-NBP1-85366-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366] - Staining of human endometrium, liver, liver cancer and tonsil using Anti-CYP2E1 antibody NBP1-85366 (A) shows similar protein distribution across tissues to independent antibody NBP1-85367 (B).](http://images.novusbio.com/fullsize/Cytochrome-P450-2E1-Antibody-Immunohistochemistry-Paraffin-NBP1-85366-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366] - Analysis in human liver and endometrium tissues. Corresponding CYP2E1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cytochrome-P450-2E1-Antibody-Immunohistochemistry-Paraffin-NBP1-85366-img0009.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366] - Staining of human tonsil shows no positivity in germinal center cells as expected.](http://images.novusbio.com/fullsize/Cytochrome-P450-2E1-Antibody-Immunohistochemistry-Paraffin-NBP1-85366-img0010.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366] - Staining of human endometrium shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Cytochrome-P450-2E1-Antibody-Immunohistochemistry-Paraffin-NBP1-85366-img0011.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366] - Staining of human liver cancer, hepatocellular carcinoma shows moderate cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/Cytochrome-P450-2E1-Antibody-Immunohistochemistry-Paraffin-NBP1-85366-img0013.jpg)
![Western Blot: SAT2 Antibody [NBP1-80722] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/SAT2-Antibody-Western-Blot-NBP1-80722-img0005.jpg "Western Blot: SAT2 Antibody [NBP1-80722] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunohistochemistry-Paraffin: SAT2 Antibody [NBP1-80722] - Staining of human colon.](http://images.novusbio.com/fullsize/SAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-80722-img0011.jpg)
![Immunohistochemistry-Paraffin: SAT2 Antibody [NBP1-80722] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/SAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-80722-img0008.jpg)
![Immunohistochemistry-Paraffin: SAT2 Antibody [NBP1-80722] - Staining of human adrenal gland shows high expression.](http://images.novusbio.com/fullsize/SAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-80722-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SAT2 Antibody [NBP1-80722] - Staining in human adrenal gland and pancreas tissues using anti-SAT2 antibody. Corresponding SAT2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-80722-img0009.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: SAT2 Antibody [NBP1-80722] - Staining of human adrenal gland, colon, pancreas and small intestine using Anti-SAT2 antibody NBP1-80722 (A) shows similar protein distribution across tissues to independent antibody NBP2-49244 (B).](http://images.novusbio.com/fullsize/SAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-80722-img0010.jpg)
![Immunohistochemistry-Paraffin: SAT2 Antibody [NBP1-80722] - Staining of human small intestine.](http://images.novusbio.com/fullsize/SAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-80722-img0012.jpg)
![Western Blot: GCLC Antibody (1A3) [NBP2-45830] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GCLC.](http://images.novusbio.com/fullsize/GCLC-Antibody-1A3-Western-Blot-NBP2-45830-img0004.jpg "Western Blot: GCLC Antibody (1A3) [NBP2-45830] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GCLC.")
![Immunohistochemistry: GCLC Antibody (1A3) [NBP2-45830] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GCLC-Antibody-1A3-Immunohistochemistry-NBP2-45830-img0003.jpg)
![Western Blot: GCLC Antibody (1A3) [NBP2-45830] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/GCLC-Antibody-1A3-Western-Blot-NBP2-45830-img0005.jpg)
![Immunohistochemistry: GCLC Antibody (1A3) [NBP2-45830] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GCLC-Antibody-1A3-Immunohistochemistry-NBP2-45830-img0001.jpg)
![Immunohistochemistry: GCLC Antibody (1A3) [NBP2-45830] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GCLC-Antibody-1A3-Immunohistochemistry-NBP2-45830-img0002.jpg)
![Western Blot: Glucosylceramidase/GBA Antibody (8O3Z7) [NBP3-15637] - Western blot analysis of extracts of various cell lines, using Glucosylceramidase beta (Glucosylceramidase/GBA) antibody (NBP3-15637) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-8O3Z7-Western-Blot-NBP3-15637-img0002.jpg "Western Blot: Glucosylceramidase/GBA Antibody (8O3Z7) [NBP3-15637] - Western blot analysis of extracts of various cell lines, using Glucosylceramidase beta (Glucosylceramidase/GBA) antibody (NBP3-15637) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: Glucosylceramidase/GBA Antibody (8O3Z7) [NBP3-15637] - Western blot analysis of extracts of Rat brain, using Glucosylceramidase beta (Glucosylceramidase/GBA) antibody (NBP3-15637) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-8O3Z7-Western-Blot-NBP3-15637-img0003.jpg)
![Immunohistochemistry: Glucosylceramidase/GBA Antibody (8O3Z7) [Glucosylceramidase/GBA] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Glucosylceramidase/GBA Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15637_rabbit-glucosylceramidase-gba-mab-8o3z7-52202517304954.jpg)
![Immunohistochemistry: Glucosylceramidase/GBA Antibody (8O3Z7) [Glucosylceramidase/GBA] - Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using Glucosylceramidase/GBA Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15637_rabbit-glucosylceramidase-gba-mab-8o3z7-52202517352739.jpg)
![Immunohistochemistry: Glucosylceramidase/GBA Antibody (8O3Z7) [Glucosylceramidase/GBA] - Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using Glucosylceramidase/GBA Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15637_rabbit-glucosylceramidase-gba-mab-8o3z7-52202517311022.jpg)
![Immunohistochemistry: Glucosylceramidase/GBA Antibody (8O3Z7) [Glucosylceramidase/GBA] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Glucosylceramidase/GBA Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15637_rabbit-glucosylceramidase-gba-mab-8o3z7-52202517311010.jpg)
![Immunohistochemistry: Glucosylceramidase/GBA Antibody (8O3Z7) [Glucosylceramidase/GBA] - Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using Glucosylceramidase/GBA Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15637_rabbit-glucosylceramidase-gba-mab-8o3z7-52202517304956.jpg)
![Immunohistochemistry: Glucosylceramidase/GBA Antibody (8O3Z7) [Glucosylceramidase/GBA] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Glucosylceramidase/GBA Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15637_rabbit-glucosylceramidase-gba-mab-8o3z7-52202517304978.jpg)
![Immunohistochemistry: Glucosylceramidase/GBA Antibody (8O3Z7) [Glucosylceramidase/GBA] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Glucosylceramidase/GBA Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15637_rabbit-glucosylceramidase-gba-mab-8o3z7-52202517352795.jpg)
![Immunohistochemistry: Glucosylceramidase/GBA Antibody (8O3Z7) [Glucosylceramidase/GBA] - Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue using Glucosylceramidase/GBA Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15637_rabbit-glucosylceramidase-gba-mab-8o3z7-52202517333847.jpg)
![Western Blot: Carbonic Anhydrase XII/CA12 Antibody (2C6) [NBP2-46042] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CA12.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-XII-CA12-Antibody-2C6-Western-Blot-NBP2-46042-img0006.jpg "Western Blot: Carbonic Anhydrase XII/CA12 Antibody (2C6) [NBP2-46042] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CA12.")
![Immunohistochemistry: Carbonic Anhydrase XII/CA12 Antibody (2C6) [NBP2-46042] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-XII-CA12-Antibody-2C6-Immunohistochemistry-NBP2-46042-img0005.jpg)
![Western Blot: Carbonic Anhydrase XII/CA12 Antibody (2C6) [NBP2-46042] - Analysis of extracts (35ug) from 9 different cell lines. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-XII-CA12-Antibody-2C6-Western-Blot-NBP2-46042-img0007.jpg)
![Immunohistochemistry: Carbonic Anhydrase XII/CA12 Antibody (2C6) [NBP2-46042] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-XII-CA12-Antibody-2C6-Immunohistochemistry-NBP2-46042-img0001.jpg)
![Immunohistochemistry: Carbonic Anhydrase XII/CA12 Antibody (2C6) [NBP2-46042] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-XII-CA12-Antibody-2C6-Immunohistochemistry-NBP2-46042-img0002.jpg)
![Immunohistochemistry: Carbonic Anhydrase XII/CA12 Antibody (2C6) [NBP2-46042] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-XII-CA12-Antibody-2C6-Immunohistochemistry-NBP2-46042-img0003.jpg)
![Immunohistochemistry: Carbonic Anhydrase XII/CA12 Antibody (2C6) [NBP2-46042] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-XII-CA12-Antibody-2C6-Immunohistochemistry-NBP2-46042-img0004.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (OTI2C6) [NBP2-46042] - Carbonic Anhydrase XII/CA12 Immunofluorescence on Human Formalin-Fixed Paraffin-Embedded (FFPE) Breast tissue. FITC Conjugated version of antibody used (NBP2-70329F). Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-46042_mouse-carbonic-anhydrase-xii-ca12-mab-oti2c6-1772024938131.jpg)
![Western Blot: Cytochrome P450 1A1 Antibody [NBP1-97762] - Staining of Human Lung lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Cytochrome-P450-1A1-Antibody-Western-Blot-NBP1-97762-img0004.jpg "Western Blot: Cytochrome P450 1A1 Antibody [NBP1-97762] - Staining of Human Lung lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A1 Antibody [NBP1-97762] - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Cytochrome-P450-1A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-97762-img0007.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 1A1 Antibody [NBP1-97762] - Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-1A1-Antibody-Immunohistochemistry-Paraffin-NBP1-97762-img0006.jpg)
![Western Blot: Cytochrome P450 1A1 Antibody [NBP1-97762] - Staining of Rat Lung lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Cytochrome-P450-1A1-Antibody-Western-Blot-NBP1-97762-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 1A1 Antibody [NBP1-97762] - Staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with Tris-EDTA pH 9, HRP-Staining. Antibody at 1:500.](http://images.novusbio.com/fullsize/Cytochrome-P450-1A1-Antibody-Immunohistochemistry-Paraffin-NBP1-97762-img0005.jpg)
![Western Blot: Carbonic Anhydrase XII/CA12 Antibody (OTI2C6) - Azide and BSA Free [NBP2-70329] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CA12.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-XII-CA12-Antibody-OTI2C6-Azide-and-BSA-Free-Western-Blot-NBP2-70329-img0002.jpg "Western Blot: Carbonic Anhydrase XII/CA12 Antibody (OTI2C6) - Azide and BSA Free [NBP2-70329] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CA12.")
![Immunohistochemistry: Carbonic Anhydrase XII/CA12 Antibody (OTI2C6) - Azide and BSA Free [NBP2-70329] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-XII-CA12-Antibody-OTI2C6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70329-img0006.jpg)
![Western Blot: Carbonic Anhydrase XII/CA12 Antibody (OTI2C6) - Azide and BSA Free [NBP2-70329] - Analysis of extracts (35ug) from 9 different cell lines. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-XII-CA12-Antibody-OTI2C6-Azide-and-BSA-Free-Western-Blot-NBP2-70329-img0001.jpg)
![Immunohistochemistry: Carbonic Anhydrase XII/CA12 Antibody (OTI2C6) - Azide and BSA Free [NBP2-70329] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-XII-CA12-Antibody-OTI2C6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70329-img0003.jpg)
![Immunohistochemistry: Carbonic Anhydrase XII/CA12 Antibody (OTI2C6) - Azide and BSA Free [NBP2-70329] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-XII-CA12-Antibody-OTI2C6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70329-img0004.jpg)
![Immunohistochemistry: Carbonic Anhydrase XII/CA12 Antibody (OTI2C6) - Azide and BSA Free [NBP2-70329] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-XII-CA12-Antibody-OTI2C6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70329-img0005.jpg)
![Immunohistochemistry: Carbonic Anhydrase XII/CA12 Antibody (OTI2C6) - Azide and BSA Free [NBP2-70329] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-XII-CA12-Antibody-OTI2C6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70329-img0007.jpg)
![Immunohistochemistry-Paraffin - Carbonic Anhydrase XII/CA12 (OTI2C6)-Azide&BSA Free [NBP2-70329] - Carbonic Anhydrase XII/CA12 Immunofluorescence on Human Formalin-Fixed Paraffin-Embedded (FFPE) Breast tissue. FITC Conjugated version of antibody used (NBP2-70329F). Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-70329_mu-carbonic-anhydrase-xii-ca12-mab-oti2c6-azide-bsa-free-immunohistochemistry-paraffin-2872023114021..jpg)
![Immunoprecipitation: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Pyruvate Carboxylase was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32722 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32722 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-32722 in HeLa cell lysate Lane 3: NBP3-32722 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-2682024158262.jpg "Immunoprecipitation: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Pyruvate Carboxylase was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32722 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32722 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-32722 in HeLa cell lysate Lane 3: NBP3-32722 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds")
![Western Blot: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Western blot analysis of Pyruvate Carboxylase on different lysates with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32722) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: MCF7 cell lysate Lane 3: A549 cell lysate Lane 4: Huh7 cell lysate Lane 5: Hela cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 130 kDa Observed band size: 130 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32722) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-27820241631640.jpg)
![Immunohistochemistry: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32722) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32722) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-29820241492649.jpg)
![Immunocytochemistry/ Immunofluorescence: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Immunocytochemistry analysis of HepG2 cells labeling Pyruvate Carboxylase with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32722) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32722) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-2920241023429.jpg)
![Flow Cytometry: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Flow cytometric analysis of PC-12 cells labeling Pyruvate Carboxylase. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32722, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-29202416372196.jpg)
![Western Blot: Cytochrome P450 2C8 Antibody [NBP1-88054] - Analysis using Anti-CYP2C8 antibody NBP1-88054 (A) shows similar pattern to independent antibody NBP1-88055 (B).](http://images.novusbio.com/fullsize/Cytochrome-P450-2C8-Antibody-Western-Blot-NBP1-88054-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Cytochrome P450 2C8 Antibody [NBP1-88054] - Analysis using Anti-CYP2C8 antibody NBP1-88054 (A) shows similar pattern to independent antibody NBP1-88055 (B).")
![Immunohistochemistry-Paraffin: Cytochrome P450 2C8 Antibody [NBP1-88054] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Cytochrome-P450-2C8-Antibody-Immunohistochemistry-Paraffin-NBP1-88054-img0016.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2C8 Antibody [NBP1-88054] - Staining of human skin shows no positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/Cytochrome-P450-2C8-Antibody-Immunohistochemistry-Paraffin-NBP1-88054-img0017.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2C8 Antibody [NBP1-88054] - Staining of human cerebellum shows no positivity in Purkinje cells as expected.](http://images.novusbio.com/fullsize/Cytochrome-P450-2C8-Antibody-Immunohistochemistry-Paraffin-NBP1-88054-img0014.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2C8 Antibody [NBP1-88054] - Staining of human Fallopian tube shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Cytochrome-P450-2C8-Antibody-Immunohistochemistry-Paraffin-NBP1-88054-img0015.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2C8 Antibody [NBP1-88055] - Staining of human cerebellum, fallopian tube, liver and skin using Anti-CYP2C8 antibody (A) NBP1-88055 shows similar protein distribution across tissues to independent antibody NBP1-88054 (B).](http://images.novusbio.com/fullsize/Cytochrome-P450-2C8-Antibody-Immunohistochemistry-Paraffin-NBP1-88055-img0017.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Cytochrome P450 2C8 Antibody [NBP1-88055] - Staining of human cerebellum, fallopian tube, liver and skin using Anti-CYP2C8 antibody (A) NBP1-88055 shows similar protein distribution across tissues to independent antibody NBP1-88054 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Cytochrome P450 2C8 Antibody [NBP1-88055] - Analysis in human liver and skin tissues. Corresponding CYP2C8 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cytochrome-P450-2C8-Antibody-Immunohistochemistry-Paraffin-NBP1-88055-img0012.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2C8 Antibody [NBP1-88055] - Staining of human cerebellum shows no positivity in Purkinje cells as expected.](http://images.novusbio.com/fullsize/Cytochrome-P450-2C8-Antibody-Immunohistochemistry-Paraffin-NBP1-88055-img0013.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2C8 Antibody [NBP1-88055] - Staining of human Fallopian tube shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Cytochrome-P450-2C8-Antibody-Immunohistochemistry-Paraffin-NBP1-88055-img0014.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2C8 Antibody [NBP1-88055] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Cytochrome-P450-2C8-Antibody-Immunohistochemistry-Paraffin-NBP1-88055-img0015.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2C8 Antibody [NBP1-88055] - Staining of human skin shows no positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/Cytochrome-P450-2C8-Antibody-Immunohistochemistry-Paraffin-NBP1-88055-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: CES2 Antibody [NBP1-84556] - Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.](http://images.novusbio.com/fullsize/CES2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84556-img0008.jpg "Immunocytochemistry/Immunofluorescence: CES2 Antibody [NBP1-84556] - Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Carboxylesterase 2/CES2 Antibody [NBP1-84556] - CES2 Antibody [NBP1-84556] - Staining in human small intestine and pancreas tissues using anti-CES2 antibody. Corresponding CES2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CES2-Antibody-Immunohistochemistry-Paraffin-NBP1-84556-img0011.jpg)
![Immunohistochemistry-Paraffin: CES2 Antibody [NBP1-84556] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/CES2-Antibody-Immunohistochemistry-Paraffin-NBP1-84556-img0009.jpg)
![Immunohistochemistry-Paraffin: CES2 Antibody [NBP1-84556] - Staining of human small intestine shows high expression.](http://images.novusbio.com/fullsize/CES2-Antibody-Immunohistochemistry-Paraffin-NBP1-84556-img0010.jpg)
![Western Blot: GCLC Antibody [NBP1-81840] - Analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/GCLC-Antibody-Western-Blot-NBP1-81840-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: GCLC Antibody [NBP1-81840] - Analysis in human cell line RT-4 and human cell line U-251 MG.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: GCLC Antibody [NBP1-81840] - Staining of human cell line A-431 shows positivity in nucleus.](http://images.novusbio.com/fullsize/GCLC-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81840-img0004.jpg)
![Immunohistochemistry-Paraffin: GCLC Antibody [NBP1-81840] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/GCLC-Antibody-Immunohistochemistry-Paraffin-NBP1-81840-img0011.jpg)
![Immunohistochemistry-Paraffin: GCLC Antibody [NBP1-81840] - Staining of human Fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/GCLC-Antibody-Immunohistochemistry-Paraffin-NBP1-81840-img0008.jpg)
![Immunohistochemistry-Paraffin: GCLC Antibody [NBP1-81840] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/GCLC-Antibody-Immunohistochemistry-Paraffin-NBP1-81840-img0009.jpg)
![Immunohistochemistry-Paraffin: GCLC Antibody [NBP1-81840] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/GCLC-Antibody-Immunohistochemistry-Paraffin-NBP1-81840-img0010.jpg)