| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89284-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59800SS | |||
| Azide and BSA Free | NBP2-80830 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87490-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87243-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77679SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56411SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70169 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-60735SS | |||
| Azide and BSA Free | NBP2-80831 | |||
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human cerebellum, kidney, liver and testis using Anti-ACAT1 antibody NBP1-89284 (A) shows similar protein distribution across tissues to independent antibody NBP1-89285 (B).](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0009.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human cerebellum, kidney, liver and testis using Anti-ACAT1 antibody NBP1-89284 (A) shows similar protein distribution across tissues to independent antibody NBP1-89285 (B).")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Western Blot: ACAT1 Antibody [NBP1-89284] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ACAT1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-89284-img0015.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: ACAT1 Antibody [NBP1-89284] - Analysis using Anti-ACAT1 antibody NBP1-89284 (A) shows similar pattern to independent antibody NBP1-89285 (B).](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-89284-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: ACAT1 Antibody [NBP1-89284] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89284-img0007.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human cerebellum shows moderate positivity in mitochondria in Purkinje cells.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0014.jpg)
![Western Blot: ACAT1 Antibody [NBP1-89284] - ACAT1 porcine cerebral cortex. Image from verified customer review.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-89284-img0008.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human testis shows strong positivity in mitochondria in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0011.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human kidney shows strong positivity in mitochondria in cells in tubules.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0012.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human liver shows strong positivity in mitochondria in hepatocytes.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0013.jpg)
![Western Blot: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Total protein from human brain (lane 1) and mouse brain (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-S100B in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Western-Blot-NBP2-45225-img0011.jpg "Western Blot: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Total protein from human brain (lane 1) and mouse brain (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-S100B in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with S100B (15F9NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-45225-img0008.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - IHC analysis of S100B in human prostate](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0010.jpg)
![Flow Cytometry: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Staining of A431 cells (1 x 10^6 cells/ml) with S100B antibody, clone 15F4.1D2 (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight 488 secondary. Shown with the secondary control (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Flow-Cytometry-NBP2-45225-img0007.jpg)
![Western Blot: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Total protein from human brain (lane 1) and mouse brain (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-S100B in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Western-Blot-NBP2-45225-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Analysis in A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with S100B (15F4NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-45225-img0003.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Analysis of S100B (15F4BN) in human brain tissue.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0004.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Analysis in human prostate tissue.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0005.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - IHC analysis of S100B (15F9NB) in human brain.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0009.jpg)
![Flow Cytometry: S100B Antibody (15F4NB) [Azide Free] [NBP2-45225] - Staining of A431 cells (1 x 10^6 cells/ml) with S100B antibody, clone 15F4.1D2 (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight 488 secondary. Shown with the secondary control (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Flow-Cytometry-NBP2-45225-img0002.jpg)
![Western Blot: LC3B Antibody (1251D) [NBP2-59800] - HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hrs. PVDF membrane was probed with 0.1 ug/mL of Rabbit Anti-LC3B Monoclonal Antibody (1251D) [Catalog# NBP2-59800] followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Knockout-Validated-NBP2-59800-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: LC3B Antibody (1251D) [NBP2-59800] - HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hrs. PVDF membrane was probed with 0.1 ug/mL of Rabbit Anti-LC3B Monoclonal Antibody (1251D) [Catalog# NBP2-59800] followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: LC3B Antibody (1251D) [NBP2-59800] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine for 24 hrs was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF (Polyvinylidene difluoride) membrane and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 2.0 ug/ml anti-LC3B Antibody (1251d) [Catalog # NBP2-59800] in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the detection of LC3I and LC3II at a molecular weight of approximately 15 kDa.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Western-Blot-NBP2-59800-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: LC3B Antibody (1251D) [NBP2-59800] - HeLa cells were treated with 50uM CQ (Chloroquine) overnight, fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-LC3B (1251d) [Catalog # NBP2-59800] at 5 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Immunocytochemistry-Immunofluorescence-NBP2-59800-img0003.jpg)
![Immunohistochemistry-Paraffin: LC3B Antibody (1251D) [NBP2-59800] - Analysis of a FFPE (Formalin-Fixed Paraffin-Embedded) tissue section of mouse brain using anti-LC3B antibody (clone 1251D) [Catalog # NBP2-59800] at 5ug/ml concentration (1:200 dilution). The primary antibody binding to LC3 in cells was detected using HRP conjugated anti-Rabbit secondary antibody with DAB (3,3'Diaminobenzidine) reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This LC3 antibody generated a diffused staining in all cell types (except the endothelial cells of blood vessels) and the signal was strongest in a subset of neuronal cells.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Immunohistochemistry-Paraffin-NBP2-59800-img0023.jpg)
![<b>Biological Strategies Validation. </b>Flow (Intracellular): LC3B Antibody (1251D) [NBP2-59800] - HeLa cells were either (A) untreated or (B) treated with 50uM chloroquine for 24 hrs. An intracellular stain was performed with anti-LC3B (1251D) antibody [Catalog # NBP2-59800] (blue) and a matched isotype control [Catalog # MAB1050] (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Flow-Intracellular-NBP2-59800-img0022.jpg)
![<b>Biological Strategies Validation. </b>Simple Western: LC3B Antibody (1251D) [NBP2-59800] - Lane view shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 50 uM Chloroquine for 18 hrs, loaded at 0.2 mg/ml. A specific band was detected for LC3B at approximately 17 kDa (as indicated) using 5 ug/mL of Rabbit Anti-Human LC3B Monoclonal Antibody (1251D) [Catalog# NBP2-59800]. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Simple-Western-NBP2-59800-img0021.jpg)
![Simple Western: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Simple Western lane view shows a specific band for ARG1 in 0.2 mg/ml of Liver (left), HepG2 (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Simple-Western-NBP1-87490-img0008.jpg "Simple Western: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Simple Western lane view shows a specific band for ARG1 in 0.2 mg/ml of Liver (left), HepG2 (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Staining in human liver and kidney tissues . Corresponding ARG1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-87490-img0019.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Analysis using Anti-ARG1 antibody NBP1-87490 (A) shows similar pattern to independent antibody NBP1-87455 (B).](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Western-Blot-NBP1-87490-img0020.jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Staining of human bone marrow shows strong cytoplasmic and nuclear positivity in a subset of hematopoietic cells.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-87490-img0015.jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Staining of human hepatocellular carcinoma shows moderate cytoplasmic and nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-87490-img0016.jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-87490-img0017.jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-87490-img0018.jpg)
![Simple Western: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Electropherogram image(s) of corresponding Simple Western lane view. Arginase 1/ARG1/liver Arginase antibody was used at 1:50 dilution on Liver and HepG2 lysate(s).](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Simple-Western-NBP1-87490-img0009.jpg)
![Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP1-87243] - Analysis in human small intestine and skeletal muscle tissues using NBP1-87243 antibody. Corresponding NAPRT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NAPRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87243-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP1-87243] - Analysis in human small intestine and skeletal muscle tissues using NBP1-87243 antibody. Corresponding NAPRT RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP1-87243] - Staining of human colon, kidney, lymph node and urinary bladder using Anti-NAPRT antibody NBP1-87243 (A) shows similar protein distribution across tissues to independent antibody NBP1-87244 (B).](http://images.novusbio.com/fullsize/NAPRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87243-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: NAPRT1 Antibody [NBP1-87243] - Analysis using Anti-NAPRT antibody NBP1-87243 (A) shows similar pattern to independent antibody NBP1-87244 (B).](http://images.novusbio.com/fullsize/NAPRT1-Antibody-Western-Blot-NBP1-87243-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: NAPRT1 Antibody [NBP1-87243] - Staining of human cell line U-251 MG shows localization to nucleoplasm, cytosol & the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NAPRT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87243-img0002.jpg)
![Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP1-87243] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/NAPRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87243-img0015.jpg)
![Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP1-87243] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NAPRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87243-img0012.jpg)
![Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP1-87243] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/NAPRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87243-img0013.jpg)
![Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP1-87243] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/NAPRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87243-img0014.jpg)
![Western Blot: IGF-I R Antibody [NBP1-77679] - Analysis of IGF-1R in 1. HeLa, 2. Ntera2, 3. A431, 4. HepG2, 5. MCF7, 6. NIH/3T3, 7. SK-BR-3 and 8. COS7](http://images.novusbio.com/fullsize/IGF-I-R-Antibody-Western-Blot-NBP1-77679-img0009.jpg "Western Blot: IGF-I R Antibody [NBP1-77679] - Analysis of IGF-1R in 1. HeLa, 2. Ntera2, 3. A431, 4. HepG2, 5. MCF7, 6. NIH/3T3, 7. SK-BR-3 and 8. COS7")
![Immunocytochemistry/Immunofluorescence: IGF-I R Antibody [NBP1-77679] - IGF-1 R antibody was tested in A431 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/IGF-I-R-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77679-img0008.jpg)
![Immunohistochemistry-Paraffin: IGF-I R Antibody [NBP1-77679] - IHC analysis of a formalin fixed paraffin embedded tissue section of the human kidney cancer xenograft using 1:200 dilution of IFG-I R antibody (NBP1-77679). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin.](http://images.novusbio.com/fullsize/IGF-I-R-Antibody-Immunohistochemistry-Paraffin-NBP1-77679-img0010.jpg)
![Simple Western: IGF-I R Antibody [NBP1-77679] - Simple Western lane view shows a specific band for IGF-1 R in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/IGF-I-R-Antibody-Simple-Western-NBP1-77679-img0007.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NB100-56411] - Analysis of GRP78/HSPA5 in A) HeLa, B) human liver, C) mouse liver, and D) rat liver lysate using NB100-56411 at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were use for this test.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NB100-56411-img0004.jpg "Western Blot: GRP78/HSPA5 Antibody [NB100-56411] - Analysis of GRP78/HSPA5 in A) HeLa, B) human liver, C) mouse liver, and D) rat liver lysate using NB100-56411 at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were use for this test.")
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NB100-56411] - Analysis of GRP78/HSPA5 in formalin-fixed, paraffin-embedded mouse liver tissue using this antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NB100-56411-img0002.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NB100-56411] - Analysis of GRP78/HSPA5 in mouse liver tissue enriched using Endoplasmic Reticulum Enrichment Kit and 1 ug/ml of NB100-56411 and 0.25 ug/ml of anti-GAPDH loading control. Lane A contains 20 ugs of whole mouse liver lysate, lane B contains 20 ugs of total ER fraction, and lane C contains 20 ugs of rough ER fraction.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NB100-56411-img0003.jpg)
![Simple Western: GRP78/HSPA5 Antibody [NB100-56411] - Simple Western lane view shows a specific band for GRP78/HSPA5 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Simple-Western-NB100-56411-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytochrome c Antibody [NBP2-21569] - Human endometrial carcinoma. Cytochrome C stained by Cytochrome C antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-Immunohistochemistry-Paraffin-NBP2-21569-img0008.jpg "Immunohistochemistry-Paraffin: Cytochrome c Antibody [NBP2-21569] - Human endometrial carcinoma. Cytochrome C stained by Cytochrome C antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Western Blot: Cytochrome C Antibody [NBP2-21569] - Sample (30 ug of whole cell lysate) A: PC-12 15% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Cytochrome-C-Antibody-Western-Blot-NBP2-21569-img0002.jpg)
![Western Blot: Cytochrome C Antibody [NBP2-21569] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 15% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Cytochrome-C-Antibody-Western-Blot-NBP2-21569-img0003.jpg)
![Western Blot: Cytochrome c Antibody [NBP2-21569] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with Cytochrome C antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident pico Western HRP Substrate.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-Western-Blot-NBP2-21569-img0011.jpg)
![Immunohistochemistry-Paraffin: Cytochrome c Antibody [NBP2-21569] - Human cervical carcinoma. Cytochrome C stained by Cytochrome C antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-Immunohistochemistry-Paraffin-NBP2-21569-img0007.jpg)
![Simple Western: Cytochrome c Antibody [NBP2-21569] - Cytochrome C Antibodies (NBP2-21569), ProteinSimple Western Blot on Jess Instrument; 1 microgram of human brain tissue lysate was tested with the antibodies diluted 10 and 20 times. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-21569_rabbit-polyclonal-cytochrome-c-antibody-simple-western-204202311476.jpg)
![Western Blot: Cytochrome c Antibody [NBP2-21569] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome c antibody (NBP2-21569) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-21569_rabbit-polyclonal-cytochrome-c-antibody-91020242063356.jpg)
![Western Blot: Cytochrome c Antibody [NBP2-21569] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome c antibody (NBP2-21569) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-21569_rabbit-polyclonal-cytochrome-c-antibody-91020242063396.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Cytochrome c Antibody [NBP2-21569] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-21569_rabbit-polyclonal-cytochrome-c-antibody-1610202419405762.jpg)
![Western Blot: IRE1 alpha Antibody Pack [NBP2-50067] - Western Blot: IRE1 alpha [p Ser724] Antibody [NB100-2323] - Western blot of anti-IRE1 alpha (pSer724) using Lot AN and AO of NB100-2323. HeLa cells were treated (+) or untreated (-) with 10 mM DTT for 60 min to activate the UPR. Total protein was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% BSA in TBST. The membrane was probed with 2.0 ug/ml antibody in 5% BSA, and detected with an anti-rabbit HRP secondary antibody using chemiluminecence. Theoretical molecular weight: 110 kDa.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Pack-Western-Blot-NBP2-50067-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: IRE1 alpha Antibody Pack [NBP2-50067] - Western Blot: IRE1 alpha [p Ser724] Antibody [NB100-2323] - Western blot of anti-IRE1 alpha (pSer724) using Lot AN and AO of NB100-2323. HeLa cells were treated (+) or untreated (-) with 10 mM DTT for 60 min to activate the UPR. Total protein was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% BSA in TBST. The membrane was probed with 2.0 ug/ml antibody in 5% BSA, and detected with an anti-rabbit HRP secondary antibody using chemiluminecence. Theoretical molecular weight: 110 kDa.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: IRE1 alpha Antibody Pack [NBP2-50067] - P-Ire1a activation correlates with WFS1 cell expression. Cryosections of retina from 12 month old Wfs1+/+ and Wfs1-/- mouse were immunostained with anti-P-Ire1a antibody (red). DAPI was used for staining of cell nuclei (blue). RPE, retinal pigment epithelium; ONL, outer nuclear layer; INL, inner nuclear layer, RGC, retinal ganglion cells. Scale bars=50 um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0097222) licensed under a CC-BY license. IRE1 alpha [p Ser724] Antibody [NB100-2323]](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Pack-Immunohistochemistry-NBP2-50067-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: IRE1 alpha Antibody Pack [NBP2-50067] - Western Blot detection of Total IRE1 Alpha in transfected Min6 cells using IRE1 alpha Antibody [NB100-2324]](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Pack-Western-Blot-NBP2-50067-img0002.jpg)
![Immunohistochemistry: IRE1 alpha Antibody Pack [NBP2-50067] - XBP1 and TG2 deficiency promote increased IRE1a protein levels in cultured VSMCs and in neointima in situ. (D) Xbp1WT and Xbp1 delta WT and (E) Tgm2 WT and Tgm2 delta carotid artery sections were analyzed by IHC for total IRE1a. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0212235) licensed under a CC-BY license. IRE1 alpha Antibody [NB100-2324]](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Pack-Immunohistochemistry-NBP2-50067-img0008.jpg)
![Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ALOX15 monoclonal antibody.(1:200)](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Western-Blot-NBP2-01740-img0012.jpg "Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ALOX15 monoclonal antibody.(1:200)")
![Immunocytochemistry/Immunofluorescence: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - COS7 cells](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Immunocytochemistry-Immunofluorescence-NBP2-01740-img0011.jpg)
![Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Staining of paraffin-embedded Human endometrium tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-01740-img0008.jpg)
![Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 15 Lipoxygenase 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-15 Lipoxygenase 1.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Western-Blot-NBP2-01740-img0001.jpg)
![Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Analysis of extracts (35ug) from 9 different cell lines by using anti-15 Lipoxygenase 1 monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Western-Blot-NBP2-01740-img0009.jpg)
![Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-01740-img0002.jpg)
![Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-01740-img0003.jpg)
![Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-01740-img0004.jpg)
![Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-01740-img0005.jpg)
![Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-01740-img0006.jpg)
![Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Staining of paraffin-embedded Human breast tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-01740-img0007.jpg)
![Western Blot: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of eIF2A [p Ser51] on THP-1 cell lysates. Lane 1: THP-1 cells, whole cell lysate, 10ug/lane Lane 2: THP-1 cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-eIF2A [p Ser51]antibody at 1/500 dilution. Anti-GAPDH antibody at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 36 kDa Observed band size: 36 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 3 minutes 43 seconds](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Western-Blot-NBP2-67353-img0011.jpg "Western Blot: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of eIF2A [p Ser51] on THP-1 cell lysates. Lane 1: THP-1 cells, whole cell lysate, 10ug/lane Lane 2: THP-1 cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-eIF2A [p Ser51]antibody at 1/500 dilution. Anti-GAPDH antibody at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 36 kDa Observed band size: 36 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 3 minutes 43 seconds")
![Immunocytochemistry/Immunofluorescence: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Staining of Hela cells (green) using NBP2-67353. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunocytochemistry-Immunofluorescence-NBP2-67353-img0008.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-elF2A [p Ser51] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0013.jpg)
![Flow Cytometry: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of Hela cells with Phospho-eIF-2a(S51) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Flow-Cytometry-NBP2-67353-img0001.jpg)
![Western Blot: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of Phospho-eIF-2a(S51) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: HUVEC cell lysateLane 2: Hela cell lysateLane 2: PC-3M cell lysate](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Western-Blot-NBP2-67353-img0009.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded human liver tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0002.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded human pancreas tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0003.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded mouse brain tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0004.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded mouse pancreas tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0005.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded mouse placenta tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0006.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using anti-eIF2A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-14, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0010.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-eIF2A [p Ser51] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0012.jpg)
![Western Blot: LC3B Antibody (1251B) [NBP2-60735] - Western blot analysis of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50uM Chloroquine for 18 hours. PVDF (polyvinylidene difluoride) membrane was probed with 0.1 ug/mL of anti-LC3B monoclonal antibody (Catalog # NBP2-60735) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Knockout-Validated-NBP2-60735-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: LC3B Antibody (1251B) [NBP2-60735] - Western blot analysis of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50uM Chloroquine for 18 hours. PVDF (polyvinylidene difluoride) membrane was probed with 0.1 ug/mL of anti-LC3B monoclonal antibody (Catalog # NBP2-60735) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Simple Western: LC3B Antibody (1251B) [NBP2-60735] - Lane view shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 50uM Chloroquine for 18 hours, loaded at 0.2 mg/mL. A specific band was detected for LC3B at approximately 17 kDa (as indicated) using 5 ug/mL of Rabbit Anti-Human/Mouse/Rat anti-LC3B Monoclonal Antibody (1251B) [Catalog # NBP2-60735]. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Simple-Western-NBP2-60735-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: LC3B Antibody (1251B) [NBP2-60735] - HeLa cells were treated with 50uM CQ (Chloroquine) overnight, fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti- [Catalog # NBP2-60735] at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Immunocytochemistry-Immunofluorescence-NBP2-60735-img0007.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: LC3B Antibody (1251B) [NBP2-60735] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine (CQ) for 24 hours was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF (Polyvinylidene difluoride) membrane and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 12.0 ug/ml anti-LC3B Antibody (1251B) [Catalog # NBP2-60735] in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Western-Blot-NBP2-60735-img0010.jpg)
![Immunohistochemistry-Paraffin: LC3B Antibody (1251B) [NBP2-60735] - Analysis of a FFPE (Formalin-Fixed Paraffin-Embedded) tissue section of mouse brain using anti-LC3B antibody (clone 1251B) [Catalog # NBP2-60735] at 5ug/ml concentration (1:200 dilution). The primary antibody binding to LC3 in cells was detected using HRP conjugated anti-Rabbit secondary antibody with DAB (3,3'Diaminobenzidine) reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This LC3 antibody generated a diffused staining in all cell types (except the endothelial cells of blood vessels) and the signal was strongest in a subset of neuronal cells. A few cells depicted LC3 puncta formation/dotted staining in apparently autophagic cells.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Immunohistochemistry-Paraffin-NBP2-60735-img0011.jpg)
![<b>Biological Strategies Validation. </b>Flow (Intracellular): LC3B Antibody (1251B) [NBP2-60735] - HeLa cells were either (A) untreated or (B) treated with 50uM chloroquine for 24 hours. An intracellular stain was performed with anti-LC3B (1251B) antibody [Catalog # NBP2-60735] (blue) and a matched isotype control [Catalog # MAB1050] (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Flow-Intracellular-NBP2-60735-img0009.jpg)