| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| DyLight 488 | NB110-37253G | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76735-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35183-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03445-20ul | |||
![Immunocytochemistry/Immunofluorescence: ACSL3 Antibody [NBP2-15252] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ACSL3 stained by ACSL3 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/ACSL3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15252-img0008.jpg "Immunocytochemistry/Immunofluorescence: ACSL3 Antibody [NBP2-15252] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ACSL3 stained by ACSL3 antibody diluted at 1:500.")
![Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP2-15252] - Human breast carcinoma. ACSL3 stained by ACSL3 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/ACSL3-Antibody-Immunohistochemistry-Paraffin-NBP2-15252-img0009.jpg)
![Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP2-15252] - IHC analysis of human breast cancer tissue section, using ACSL3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer for 15 min.](http://images.novusbio.com/fullsize/ACSL3-Antibody-Immunohistochemistry-Paraffin-NBP2-15252-img0001.jpg)
![Simple Western: ACSL3 Antibody [NBP2-15252] - Mouse brain (hippocampus) tissue. Simple Western image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ACSL3-Antibody-Simple-Western-NBP2-15252-img0005.jpg)
![Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP2-15252] Mouse stomach. ACSL3 stained by ACSL3 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/antibody/nbp2-15252_rabbit-polyclonal-acsl3-antibody-immunohistochemistry-paraffin-118202381849..jpg)
![Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP2-15252] - Rat colon.ACSL3 stained by ACSL3 antibody diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/antibody/nbp2-15252_rabbit-polyclonal-acsl3-antibody-immunohistochemistry-paraffin-11820238229..jpg)
![Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP2-15252] - ACSL3 antibody detects ACSL3 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse stomach.ACSL3 stained by ACSL3 antibody (NBP2-15252) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15252_rabbit-polyclonal-acsl3-antibody-910202420115023.jpg)
![Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP2-15252] - ACSL3 antibody detects ACSL3 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded mouse stomach.ACSL3 stained by ACSL3 antibody (NBP2-15252) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15252_rabbit-polyclonal-acsl3-antibody-91020242014680.jpg)
![Western Blot: HSL Antibody [NB110-37253] - Detection of HSL using NB110-37253. Lane 1: Liver extract (negative control) Lane 2: Fat extract (10ug) (positive sample).](http://images.novusbio.com/fullsize/HSL-Antibody-Western-Blot-NB110-37253-img0002.jpg "Western Blot: HSL Antibody [NB110-37253] - Detection of HSL using NB110-37253. Lane 1: Liver extract (negative control) Lane 2: Fat extract (10ug) (positive sample).")
![Western Blot: ACSL3 Antibody [H00002181-B01P] - Western Blot analysis of ACSL3 expression in transfected 293T cell line by ACSL3 MaxPab polyclonal antibody. Lane 1: ACSL3 transfected lysate (79. 2 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ACSL3-Antibody-Western-Blot-H00002181-B01P-img0003.jpg "Western Blot: ACSL3 Antibody [H00002181-B01P] - Western Blot analysis of ACSL3 expression in transfected 293T cell line by ACSL3 MaxPab polyclonal antibody. Lane 1: ACSL3 transfected lysate (79. 2 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: ACSL3 Antibody [H00002181-B01P] - Analysis of purified antibody to ACSL3 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/ACSL3-Antibody-Immunocytochemistry-Immunofluorescence-H00002181-B01P-img0001.jpg)
![Western Blot: Protein Phosphatase 1C gamma Antibody [NBP1-32858] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PP1C gamma antibody [N1C2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Protein-Phosphatase-1C-gamma-Antibody-Western-Blot-NBP1-32858-img0012.jpg "Western Blot: Protein Phosphatase 1C gamma Antibody [NBP1-32858] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PP1C gamma antibody [N1C2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Protein Phosphatase 1C gamma Antibody [NBP1-32858] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Protein-Phosphatase-1C-gamma-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32858-img0008.jpg)
![Immunohistochemistry-Paraffin: Protein Phosphatase 1C gamma Antibody [NBP1-32858] - SAS xenograft, using PPP1CC antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Protein-Phosphatase-1C-gamma-Antibody-Immunohistochemistry-Paraffin-NBP1-32858-img0009.jpg)
![Western Blot: Protein Phosphatase 1C gamma Antibody [NBP1-32858] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Protein-Phosphatase-1C-gamma-Antibody-Western-Blot-NBP1-32858-img0010.jpg)
![Western Blot: ACSL3 Antibody [NBP1-89268] - Analysis in human cell lines SK-MEL-30 and U-251MG using anti-ACSL3 antibody. Corresponding ACSL3 RNA-seq data are presented for the same cell lines. Loading control: anti-HDAC1.](http://images.novusbio.com/fullsize/ACSL3-Antibody-Western-Blot-NBP1-89268-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: ACSL3 Antibody [NBP1-89268] - Analysis in human cell lines SK-MEL-30 and U-251MG using anti-ACSL3 antibody. Corresponding ACSL3 RNA-seq data are presented for the same cell lines. Loading control: anti-HDAC1.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: ACSL3 Antibody [NBP1-89268] - Analysis in control (vector only transfected HEK293T lysate) and ACSL3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/ACSL3-Antibody-Western-Blot-NBP1-89268-img0013.jpg)
![Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP1-89268] - Staining of human tonsil shows no positivity in lymphoid cells as expected.](http://images.novusbio.com/fullsize/ACSL3-Antibody-Immunohistochemistry-Paraffin-NBP1-89268-img0010.jpg)
![Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP1-89268] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/ACSL3-Antibody-Immunohistochemistry-Paraffin-NBP1-89268-img0007.jpg)
![Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP1-89268] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ACSL3-Antibody-Immunohistochemistry-Paraffin-NBP1-89268-img0008.jpg)
![Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP1-89268] - Staining of human stomach shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ACSL3-Antibody-Immunohistochemistry-Paraffin-NBP1-89268-img0009.jpg)
![Western Blot: Hormone-sensitive Lipase/HSL Antibody [NBP1-76735] - Human lymph node tissue lysate with HSL antibody at (A) 0.5 and (B) 1 ug/ml.](http://images.novusbio.com/fullsize/Hormone-sensitive-Lipase-HSL-Antibody-Western-Blot-NBP1-76735-img0003.jpg "Western Blot: Hormone-sensitive Lipase/HSL Antibody [NBP1-76735] - Human lymph node tissue lysate with HSL antibody at (A) 0.5 and (B) 1 ug/ml.")
![Immunohistochemistry-Paraffin: Hormone-sensitive Lipase/HSL Antibody [NBP1-76735] - Human lymph node tissue with HSL antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/Hormone-sensitive-Lipase-HSL-Antibody-Immunohistochemistry-Paraffin-NBP1-76735-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Hormone-sensitive Lipase/HSL Antibody - BSA Free [NBP1-76735] - Immunofluorescence of Hormone-sensitive Lipase/HSL in Human Lymph Node cells with Hormone-sensitive Lipase/HSL antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76735_rabbit-polyclonal-hormone-sensitive-lipase-hsl-antibody-271120242011550.jpg)
![Immunohistochemistry: ACSL3 Antibody [NBP3-35183] - Immunohistochemistry analysis of paraffin-embedded Human mammary cancer using ACSL3 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35183_rabbit-acsl3-pab-91220241418187.jpg "Immunohistochemistry: ACSL3 Antibody [NBP3-35183] - Immunohistochemistry analysis of paraffin-embedded Human mammary cancer using ACSL3 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Western Blot: ACSL3 Antibody [NBP3-35183] - Western blot analysis of various lysates using ACSL3 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35183_rabbit-acsl3-pab-912202414123889.jpg)
![Immunohistochemistry: ACSL3 Antibody [NBP3-35183] - Immunohistochemistry analysis of paraffin-embedded Human stomach using ACSL3 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35183_rabbit-acsl3-pab-912202414112893.jpg)
![Immunohistochemistry: ACSL3 Antibody [NBP3-35183] - Immunohistochemistry analysis of paraffin-embedded Rat heart using ACSL3 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35183_rabbit-acsl3-pab-91220241515287.jpg)
![Immunohistochemistry: ACSL3 Antibody [NBP3-35183] - Immunohistochemistry analysis of paraffin-embedded Mouse heart using ACSL3 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35183_rabbit-acsl3-pab-91220241415436.jpg)
![Western Blot: Protein Phosphatase 1C gamma Antibody [NBP3-03445] - Analysis of extracts of various cell lines, using Protein Phosphatase 1C gamma antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Protein-Phosphatase-1C-gamma-Antibody-Western-Blot-NBP3-03445-img0005.jpg "Western Blot: Protein Phosphatase 1C gamma Antibody [NBP3-03445] - Analysis of extracts of various cell lines, using Protein Phosphatase 1C gamma antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![<b>Genetic Strategies Validation. </b>Western Blot: Protein Phosphatase 1C gamma Antibody [NBP3-03445] - Analysis of extracts from normal (control) and PPP1CC knockout (KO) HeLa cells, using Protein Phosphatase 1C gamma antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Protein-Phosphatase-1C-gamma-Antibody-Knockout-Validated-NBP3-03445-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Protein Phosphatase 1C gamma Antibody - BSA Free [NBP3-03445] - Immunofluorescence analysis of H9C2 cells using [KO Validated] Protein Phosphatase 1C gamma Rabbit pAb (A4035) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03445_rabbit-polyclonal-protein-phosphatase-1c-gamma-antibody-52202519313434.jpg)
![Immunocytochemistry/ Immunofluorescence: Protein Phosphatase 1C gamma Antibody - BSA Free [NBP3-03445] - Immunofluorescence analysis of U2OS cells using [KO Validated] Protein Phosphatase 1C gamma Rabbit pAb (A4035) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03445_rabbit-polyclonal-protein-phosphatase-1c-gamma-antibody-5220251931398.jpg)
![Immunocytochemistry/ Immunofluorescence: Protein Phosphatase 1C gamma Antibody - BSA Free [NBP3-03445] - Immunofluorescence analysis of L929 cells using [KO Validated] Protein Phosphatase 1C gamma Rabbit pAb (A4035) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03445_rabbit-polyclonal-protein-phosphatase-1c-gamma-antibody-52202519313475.jpg)
![Western Blot: Hormone-sensitive Lipase/HSL [p Ser660] Antibody [NBP3-05459] - Analysis of extracts of NIH/3T3 cells, using Phospho-HSL-S660 antibody at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (10 uM) at 37c for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Hormone-sensitive-Lipase-HSL-[p-Ser660]-Antibody-Western-Blot-NBP3-05459-img0001.jpg "Western Blot: Hormone-sensitive Lipase/HSL [p Ser660] Antibody [NBP3-05459] - Analysis of extracts of NIH/3T3 cells, using Phospho-HSL-S660 antibody at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (10 uM) at 37c for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.Detection: ECL Basic Kit. Exposure time: 180s.")
![Western Blot: Protein Phosphatase 1C gamma Antibody [NB110-40551] - Whole cell lysate (50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB110-40551 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B). PPP1CB was also immunoprecipitated by NB110-40551.](http://images.novusbio.com/fullsize/Protein-Phosphatase-1C-gamma-Antibody-Western-Blot-NB110-40551-img0002.jpg "Western Blot: Protein Phosphatase 1C gamma Antibody [NB110-40551] - Whole cell lysate (50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB110-40551 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B). PPP1CB was also immunoprecipitated by NB110-40551.")
![Immunohistochemistry-Paraffin: Protein Phosphatase 1C gamma Antibody [NB110-40551] - Human breast carcinoma. Antibody: Affinity purified rabbit anti- PPP1CC used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Protein-Phosphatase-1C-gamma-Antibody-Immunohistochemistry-Paraffin-NB110-40551-img0010.jpg)
![Western Blot: Hormone-sensitive Lipase/HSL [p Ser563] Antibody [NBP3-05457] - Western blot analysis of extracts of various cell lines, using Hormone-sensitive Lipase/HSL antibody (NBP3-05457) at 1:1000 dilution.3T3L1 cells were treated by lambda-PP mixed solution (1ul) at 30C for 30 minutes.H9C2 cells were treated with Isoproterenol (10 uM for 20 min) at 37C. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Hormone-sensitive Lipase-HSL [p Ser563] Antibody-Western Blot-NBP3-05457-img0001.jpg "Western Blot: Hormone-sensitive Lipase/HSL [p Ser563] Antibody [NBP3-05457] - Western blot analysis of extracts of various cell lines, using Hormone-sensitive Lipase/HSL antibody (NBP3-05457) at 1:1000 dilution.3T3L1 cells were treated by lambda-PP mixed solution (1ul) at 30C for 30 minutes.H9C2 cells were treated with Isoproterenol (10 uM for 20 min) at 37C. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Western Blot: Hormone-sensitive Lipase/HSL [p Ser563] Antibody - Azide and BSA Free [Hormone-sensitive Lipase/HSL] - Western blot analysis of lysates from Mouse brown fat using Hormone-sensitive Lipase/HSL Rabbit pAb at 1:5000 dilution incubated overnight at 4C. Mouse brown fat were treated by lambda-PP mixed solution (1 ul) at 30C for 30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 30 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-05457_rabbit-polyclonal-hormone-sensitive-lipase-hsl-p-ser563-antibody-52202516334111.jpg)
![Immunohistochemistry: ACSL3 Antibody (A8F6-R) [NBP3-31994] - Immunohistochemical analysis of paraffin-embedded human stomach tissue with Mouse anti-ACSL3 antibody (NBP3-31994) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-31994) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-31994_mouse-acsl3-mab-a8f6-r-29820241435265.jpg "Immunohistochemistry: ACSL3 Antibody (A8F6-R) [NBP3-31994] - Immunohistochemical analysis of paraffin-embedded human stomach tissue with Mouse anti-ACSL3 antibody (NBP3-31994) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-31994) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: ACSL3 Antibody (A8F6-R) [NBP3-31994] - Western blot analysis of ACSL3 on different lysates with Mouse anti-ACSL3 antibody (NBP3-31994) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: A431 cell lysate Lane 4: 293T cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 80 kDa Observed band size: 70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-31994) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-31994_mouse-acsl3-mab-a8f6-r-3920248485019.jpg)