| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-25195SS | |||
| Azide and BSA Free | NBP2-80659 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24827SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77067SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64021-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18816SS | |||
| Azide and BSA Free | NBP3-18817 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-236SS | |||
![Flow Cytometry: CD8 Antibody (RPA-T8) [NBP2-25195] - CD8 alpha Antibody (RPA-T8) [NBP2-25195] - Analysis using PerCP conjugate of NBP2-25195. A cell surface stain was performed on hPBMCs with CD8 Alpha antibody (RPA-T8) NBP2-25195 (top image) and a matched isotype control NBP2-27287 (bottom image). Cells were incubated in an antibody dilution of 1:200 for 20 minutes at room temperature. Both antibodies were conjugated to PerCP. A co-stain was also performed using CD3 antibody NBP2-24867AF488.](http://images.novusbio.com/fullsize/CD8-Antibody-RPA-T8-Flow-Cytometry-NBP2-25195-img0015.jpg "Flow Cytometry: CD8 Antibody (RPA-T8) [NBP2-25195] - CD8 alpha Antibody (RPA-T8) [NBP2-25195] - Analysis using PerCP conjugate of NBP2-25195. A cell surface stain was performed on hPBMCs with CD8 Alpha antibody (RPA-T8) NBP2-25195 (top image) and a matched isotype control NBP2-27287 (bottom image). Cells were incubated in an antibody dilution of 1:200 for 20 minutes at room temperature. Both antibodies were conjugated to PerCP. A co-stain was also performed using CD3 antibody NBP2-24867AF488.")
![Flow Cytometry: CD8 Antibody (RPA-T8) [NBP2-25195] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-25195. Staining of CD8 alpha in human PBMC using CD8 alpha antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/CD8-Antibody-RPA-T8-Flow-Cytometry-NBP2-25195-img0012.jpg)
![Flow Cytometry: CD8 Antibody (RPA-T8) [NBP2-25195] - Analysis using the DyLight 405 conjugate of NBP2-25195. Staining of CD8 alpha in human PBMCs using anti-CD8 alpha antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/CD8-Antibody-RPA-T8-Flow-Cytometry-NBP2-25195-img0013.jpg)
![Flow (Cell Surface): CD8 Antibody (RPA-T8) [NBP2-25195] - CD8 alpha Antibody (RPA-T8) [NBP2-25195] - Analysis using the PE conjugate of NBP2-25195. Staining of Human peripheral blood mononuclear cells. Image from verified customer review.](http://images.novusbio.com/fullsize/CD8-Antibody-RPA-T8-Flow-Cell-Surface-NBP2-25195-img0014.jpg)
![Flow Cytometry: CD8 Antibody (RPA-T8) - Azide and BSA Free [NBP2-25195] - Analysis using the PE conjugate of NBP2-25195. Staining of CD8 in 10^6 human PBMC using 10 ul (0.1 ug) of this antibody. The shaded histogram represents human PBMC alone; green represents mouse isotype control, red represents human CD8 this antibody.](http://images.novusbio.com/fullsize/nbp2-25195_mouse-monoclonal-cd8-antibody-rpa-t8-imgenex-img-5917a-25ug-1772024104463.jpg)
![Flow Cytometry: CD8 Antibody (RPA-T8) - Azide and BSA Free [NBP2-25195] - Analysis using the FITC conjugate of NBP2-25195. Staining of CD8 in human PBMCs using 0.25 ug of (clone RPA-T8) per approximately 1x10^6 cells. Green represents isotype control this antibody, red represents anti-CD8 FITC antibody.](http://images.novusbio.com/fullsize/nbp2-25195_mouse-monoclonal-cd8-antibody-rpa-t8-imgenex-img-5917a-25ug-17720241044620.jpg)
![Simple Western: VPS34 Antibody [NB110-87320] - Simple Western lane view shows a specific band for VPS34 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/VPS34-Antibody-Simple-Western-NB110-87320-img0006.jpg "Simple Western: VPS34 Antibody [NB110-87320] - Simple Western lane view shows a specific band for VPS34 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: VPS34 Antibody [NB110-87320] - HepG2 whole cell lysates.](http://images.novusbio.com/fullsize/VPS34-Antibody-Western-Blot-NB110-87320-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: VPS34 Antibody [NB110-87320] - VPS34 antibody was tested at 1:200 in HeLa cells with FITC (green). Nuclei (Blue) were counterstained with Dapi (blue).](http://images.novusbio.com/fullsize/VPS34-Antibody-Immunocytochemistry-Immunofluorescence-NB110-87320-img0005.jpg)
![Western Blot: VPS34 Antibody [NB110-87320] - Celastrol regulates autophagy- & mitophagy-related gene expressions in neurons. SH-SY5Y cells treated with 0.1–1 μM celastrol for 4 h. (A) Real-time quantitative PCR results show that 1 μM celastrol treatment for 4 h enhanced mRNA expressions of phagophore induction genes Beclin 1 & Ambra1, phagophore elongation genes Atg7 & Atg12, & vacuole formation genes LC3A & Atg4B. (B) Western blot results of SH-SY5Y treated with 100–500 nM celastrol show celastrol (500 nM) increased in protein expressions of Beclin 1 & Vps34 (phagophore induction), Atg7 (phagophore elongation), LC3-II (vacuole formation), DJ-1, & PINK1 (mitophagy). Celastrol (500 nM) suppressed LRRK2 expression. (C) MPTP (10 mg/kg/day for 3 days, i.p.) but not celastrol (3 mg/kg/day for 3 days, i.p.) caused dopaminergic nerve terminal degeneration (tyrosine hydroxylase↓) & mitophagy inactivation (DJ-1↓ & PINK1↓) in the striatum of mice as compared to saline control. Celastrol cotreatment with MPTP reversed it as compared to MPTP. Data are given as the mean ± SEM (n = 3–5). p-value was determined using the Kruskal–Wallis test followed by Dunn’s multiple comparison post hoc test. * p < 0.05 compared with controls. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31906147), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-87320_rabbit-polyclonal-vps34-antibody-310202416205164.jpg)
![Western Blot: Complement C3 Antibody [NBP1-32080] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with C3 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Western-Blot-NBP1-32080-img0023.jpg "Western Blot: Complement C3 Antibody [NBP1-32080] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with C3 antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Complement C3 Antibody [NBP1-32080] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C3 protein stained by C3 antibody [C3], C-term diluted at 1:200. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32080-img0019.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-32080] - Mouse brain. C3 antibody [C3], C-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-32080-img0015.jpg)
![Immunoprecipitation: Complement C3 Antibody [NBP1-32080] - Immunoprecipitation of C3 protein from HepG2 whole cell extracts using 5 ug of C3 antibody [C3], C-term.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunoprecipitation-NBP1-32080-img0017.jpg)
![Human plasma (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Complement C3 antibody [C3], C-term (NBP1-32080) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32080_rabbit-polyclonal-complement-c3-antibody-western-blot-1411202518442224.jpg)
![Western Blot: TLR5 Antibody [NBP2-24827] - THP-1 cell lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/TLR5-Antibody-Western-Blot-NBP2-24827-img0004.jpg "Western Blot: TLR5 Antibody [NBP2-24827] - THP-1 cell lysate. Image from verified customer review.")
![Immunohistochemistry-Paraffin: TLR5 Antibody [NBP2-24827] - Analysis of TLR5 in FFPE human stomach tumor tissue using TLR5 antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/TLR5-Antibody-Immunohistochemistry-Paraffin-NBP2-24827-img0002.jpg)
![Flow Cytometry: TLR5 Antibody [NBP2-24827] - Intracellular flow analysis of TLR5 in Balb/c mouse splenocytes using TLR5 antibody at 2 ug/10^6 cells. Shaded histogram: cells without antibody. Green: rabbit IgG isotype control. Red: TLR5 antibody. Goat anti-rabbit PE was used as secondary antibody.](http://images.novusbio.com/fullsize/TLR5-Antibody-Flow-Cytometry-NBP2-24827-img0003.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - Loading: 15 ug of lysates per lane. Antibodies: NBP1-7706 (1 ug/mL), NBP1-76480 (1 ug/mL), and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0013.jpg "Western Blot: CXCR4 Antibody [NBP1-77067] - Loading: 15 ug of lysates per lane. Antibodies: NBP1-7706 (1 ug/mL), NBP1-76480 (1 ug/mL), and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP")
![Immunohistochemistry: CXCR4 Antibody - BSA Free [NBP1-77067] - Validation of CXCR4 in Human Spleen. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CXCR4 antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A Goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77067_rabbit-polyclonal-cxcr4-antibody-2711202420324853.gif)
![<b>Genetic Strategies Validation. </b>Flow Cytometry: CXCR4 Antibody [NBP1-77067] - Astrocytes from wild-type or CXCR4 knockout mice were stained with primary antibodies against CXCR4 and FITClabeled secondary antibodies, and subsequently subjected to flow cytometry. CXCR4-/- astrocytes (red) showed loss of CXCR4 cell-surface expression compared with wild-type cells (black).](http://images.novusbio.com/fullsize/CXCR4-Antibody-Flow-Cytometry-NBP1-77067-img0010.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - HeLa whole cell lysate with CXCR4 antibody at 0.5 ug/ml.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0001.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - Recombinant Protein Test: Loading: CXCR4 partial recombinant protein (H00007852-Q01). Lane 1: Anti-CXCR4 antibody (0.1 ug/mL) 1 h incubation at RT in 5% NFDM/TBST. Lane 2: Coomassie blue staining.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0007.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - CXCR4 protein was detected in the human metastatic melanoma cell lines and human melanoma cell line (colo38), but not in the human primary melanocytes (MPR1) with anti-CXCR4 antibodies.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0008.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - U87MG and U87MG-CXCR4 extracts were included as negative and positive controls, respectively, for CXCR4 detection with anti-CXCR4 antibodies.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0009.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - Loading: Lysates/proteins at 20 ug per lane. Antibodies: NBP1-7706 (2 ug/mL) or NBP1-76480 (2 ug/mL). 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CXCR4 Antibody [NBP1-77067] - HeLa cells were transfected with control siRNAs (lane 1) or CXCR4 siRNAs (lane 2) Loading: 10 ug of HeLa whole cell lysates per lane. Antibodies: NBP1-7706 (2 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0012.jpg)
![Flow Cytometry: CXCR4 Antibody [NBP1-77067] - Analysis of HeLa cells using CXCR4 antibody at 0.1 ug/ml. Purple: cells without staining, Green: Isotype control. Red : CXCR4 antibody.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Flow-Cytometry-NBP1-77067-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody - BSA Free [NBP1-77067] - Validation of CXCR4 in HeLa Cells. Immunocytochemical analysis of HeLa cells using anti-CXCR4 antibody at 2 u/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77067_rabbit-polyclonal-cxcr4-antibody-2711202419313214.gif)
![Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody - BSA Free [NBP1-77067] - Validation of CXCR4 in HeLa Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling CXCR4 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on HeLa cells.](http://images.novusbio.com/fullsize/nbp1-77067_rabbit-polyclonal-cxcr4-antibody-2711202420414588.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: CD8 Antibody (12.C7) [NB100-64021] - Human bile duct cancer tissue. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD8-Antibody-12-C7-Immunohistochemistry-Paraffin-NB100-64021-img0003.jpg)
![Flow Cytometry: CD8 alpha Antibody (12.C7) [NB100-64021] - Rabbit peripheral blood lymphocytes stained with Mouse anti Rabbit CD8 Alpha followed by Goat anti Mouse IgG (H/L):FITC](http://images.novusbio.com/fullsize/CD8-alpha-Antibody-12-C7-Flow-Cytometry-NB100-64021-img0001.jpg)
![Western Blot: DARC Antibody [NB100-2421] - DARC (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/DARC-Antibody-Western-Blot-NB100-2421-img0007.jpg "Western Blot: DARC Antibody [NB100-2421] - DARC (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: DARC Antibody [NB100-2421] - Staining of paraffin embedded Human Lung. Microwaved antigen retrieval with citrate buffer Ph 6, AP-staining.](http://images.novusbio.com/fullsize/DARC-Antibody-Immunohistochemistry-Paraffin-NB100-2421-img0009.jpg)
![Flow Cytometry: DARC Antibody [NB100-2421] - analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/DARC-Antibody-Flow-Cytometry-NB100-2421-img0010.jpg)
![Immunohistochemistry-Paraffin: DARC Antibody [NB100-2421] - DARC (3ug/ml) staining of paraffin embedded human kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/DARC-Antibody-Immunohistochemistry-Paraffin-NB100-2421-img0008.jpg)
![Western Blot: TLR3 [p Tyr759] Antibody [NBP2-24904] - Image from verified customer review.](http://images.novusbio.com/fullsize/TLR3-[p-Tyr759]-Antibody-Western-Blot-NBP2-24904-img0003.jpg "Western Blot: TLR3 [p Tyr759] Antibody [NBP2-24904] - Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: TLR3 [p Tyr759] Antibody [NBP2-24904] - Analysis of IgG, AID and TLR3 (A) and a hyper-IgM (HIGM) syndrome patient (B and C). A and B: Paraformaldehyde-fixed, frozen tonsillar mucosa tissue sections. C. Paraformaldehyde-fixed, mucosal B cells from the colon lamina propria of the HIGM patient. Arrowheads indicate cells co-expressing phospho TLR3, AID, and IgG. Data courtesy of W. Xu. et. al., and the Journal of Immunology](http://images.novusbio.com/fullsize/TLR3-[p-Tyr759]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24904-img0002.jpg)
![Western Blot: STING/TMEM173 Antibody (2922D) [NBP3-18816] - Western blot shows cell lysates K562, THP-1, C2C12, and C6. Membrane was probed with 1 ug/mL of (Catalog # NBP3-18816) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for STING/TMEM173 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. Internally validated Western blot.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-2922D-Western-Blot-NBP3-18816-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: STING/TMEM173 Antibody (2292D) [NBP3-18816] - STING/TMEM173 was detected in immersion fixed U937 human myeloid leukaemia cell line but is not detected in Daudi cell line using Rabbit Anti-Human STING/TMEM173 Monoclonal Antibody (Catalog # NBP3-18816) at 1 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-2292D-Immunocytochemistry-Immunofluorescence-NBP3-18816-img0002.jpg)
![Immunohistochemistry: STING/TMEM173 Antibody (2292D) [NBP3-18816] - STING/TMEM173 was detected in immersion fixed paraffin-embedded sections of mouse lung tissue using Rabbit Anti-Human STING/TMEM173 Monoclonal Antibody at 0.5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to pneumocytes and alveolar cells. Staining with VisUCyte HRP Polymer Detection Reagents.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-2292D-Immunohistochemistry-NBP3-18816-img0001.jpg)
![Simple Western: STING/TMEM173 Antibody (2922D) [NBP3-18816] - Simple Western Lane view shows K562 and THP-1 whole cell lysate (WCL). A specific band was detected for hSTING/TMEM173 antibody (NBP3-18816) at approximately 44 kDa (as indicated) using 10 ug/mL of hSTING/TMEM173 antibody. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-2922D-Simple-Western-NBP3-18816-img0004.jpg)
![Western Blot: Survivin [p Thr34] Antibody [NB500-236] - Western blot analysis using [NB500-236]. Lane 1: Phosphorylated Survivin protein and Lane 2: Non-phosphorylated Survivin protein. Note: theoretical molecular weight of Survivin Antibody: 16 kDa.](http://images.novusbio.com/fullsize/Survivin-[p-Thr34]-Antibody-Western-Blot-NB500-236-img0003.jpg "Western Blot: Survivin [p Thr34] Antibody [NB500-236] - Western blot analysis using [NB500-236]. Lane 1: Phosphorylated Survivin protein and Lane 2: Non-phosphorylated Survivin protein. Note: theoretical molecular weight of Survivin Antibody: 16 kDa.")
![Immunocytochemistry/Immunofluorescence: Survivin [p Thr34] Antibody [NB500-236] - Immunocytochemical analysis using Survivin [p Thr34] Antibody [NB500-236] in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Survivin-[p-Thr34]-Antibody-Immunocytochemistry-Immunofluorescence-NB500-236-img0004.jpg)
![Western Blot: Survivin [p Thr34] Antibody [NB500-236] - Combination therapy with TiOxNPs & IR suppressed the aggressiveness of xenografts in dissociated MIA PaCa sphere cell-bearing mice. A HE staining & IHC analysis of p-H2AX, c-caspase-3, ki67, PCNA, snail/Slug, & vimentin in the indicated groups. Scale bar = 50 μm. n=5. B Western blot for expression of p-H2AX, c-caspase-3, p-survivin, snail/Slug, & vimentin in xenografts of dissociated MIA PaCa sphere-bearing cells treated with TiOxNPs and/or irradiation. C In vivo apoptosis marker TUNEL assay in xenografts of dissociated MIA PaCa sphere-bearing cells treated with TiOxNPs and/or irradiation. n=5. Data are shown as the mean ± standard deviation. ns, not significant. ****p < 0.0001. Scale bar = 200 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35428310), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-236_rabbit-polyclonal-survivin-p-thr34-antibody-31020241539717.jpg)
![Survivin [p Thr34] was detected in immersion fixed HeLa human cervix adenocarcinoma cell line using Rabbit anti-Survivin [p Thr34] Antigen Affinity Purified Polyclonal Antibody (Catalog # NB500-236) at 2.0 µg/mL overnight at 4C. Cells were stained using DyLight 488-conjugated Anti-Rabbit IgG (H+L) Cross-Absorbed Secondary Antibody (green), and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/antibody/nb500-236_rabbit-polyclonal-survivin-p-thr34-antibody-immunocytochemistry-immunofluorescence-2662025104450..jpg)
![Survivin [p Thr34] was detected in immersion fixed NIH-3T3 Mouse fibroblast cell line using Rabbit anti Survivin [p Thr34] Antigen Affinity Purified Polyclonal Antibody (Catalog # NB500-236) at 2.0 µg/mL overnight at 4C. Cells were stained using DyLight 488-conjugated Anti-Rabbit IgG (H+L) Cross-Absorbed Secondary Antibody (green), and counterstained with DAPI (blue). Cells in various stages of mitosis were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/antibody/nb500-236_rabbit-polyclonal-survivin-p-thr34-antibody-immunocytochemistry-immunofluorescence-2662025104618..jpg)
![Western Blot: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Samples: Whole cell lysate (50 ug) from HeLa, RKO, MCF-7, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PBEF antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-Western-Blot-NB100-594-img0015.jpg "Western Blot: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Samples: Whole cell lysate (50 ug) from HeLa, RKO, MCF-7, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PBEF antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Section of human head and neck squamous cell carcinoma. Antibody: Affinity purified rabbit anti-PBEF used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-Immunohistochemistry-Paraffin-NB100-594-img0016.jpg)
![Western Blot: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PBEF antibody used for IP at 6 ug per reaction. PBEF was also immunoprecipitated by a previous lot of this antibody and rabbit anti-Visfatin antibody. For blotting immunoprecipitated PBEF, Antibody was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-Western-Blot-NB100-594-img0014.jpg)
![Western Blot: TSLP Antibody [NB110-55234] - Total protein levels of TSLP in patients with AERD and subjects with CRS measured by using Western blotting of 3 representative samples per group. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/TSLP-Antibody-Western-Blot-NB110-55234-img0007.jpg)
![Western Blot: TSLP Antibody [NB110-55234] - Western Blot Validation in Human Heart (Lane 1) and Human Prostate (Lane 2).Loading: 15 ug of lysates per lane. Antibodies: TSLP NB110-55234 (4 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/TSLP-Antibody-Western-Blot-NB110-55234-img0010.jpg)
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: TSLP Antibody [NB110-55234] - Western Blot Validation of TSLP with Human Recombinant protein. Loading: 30ng of TSLP partial human recombinant protein per lane. Antibodies: TSLP NB110-55234 (Lane 1: 0.25 ug/mL, Lane 2: 0.5 ug/mL, Lane 3: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/TSLP-Antibody-Western-Blot-NB110-55234-img0011.jpg)
![Western Blot: TSLP Antibody - BSA Free [NB110-55234] - Validation in Mouse A-20 Cell Line. Loading: 15 ug of lysates per lane.Antibodies: TSLP (A: 0.5 ug/mL, B: 1 ug/mL, C: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nb110-55234_rabbit-polyclonal-tslp-antibody-271120242013258.jpg)
![Immunocytochemistry/ Immunofluorescence: TSLP Antibody - BSA Free [NB110-55234] - Validation of TSLP in Human Brain Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Brain Tissue labeling TSLP with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nb110-55234_rabbit-polyclonal-tslp-antibody-2711202419463444.jpg)
![Immunohistochemistry: TSLP Antibody - BSA Free [NB110-55234] - Validation of TSLP in Human Brain Tissue. Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using anti-TSLP antibody at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nb110-55234_rabbit-polyclonal-tslp-antibody-2711202420333823.jpg)