| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52680-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88966-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57554-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84576-25ul | |||
![Western Blot: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of DKK1 expression in U-2 OS (Cat # L022V1).](http://images.novusbio.com/fullsize/Dkk-1-Antibody-2A5-Western-Blot-H00022943-M11-img0012.jpg "Western Blot: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of DKK1 expression in U-2 OS (Cat # L022V1).")
![Immunohistochemistry-Paraffin: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of monoclonal antibody to DKK1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/Dkk-1-Antibody-2A5-Immunohistochemistry-Paraffin-H00022943-M11-img0009.jpg)
![Immunoprecipitation: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of DKK1 transfected lysate using anti-DKK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with DKK1 rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Dkk-1-Antibody-2A5-Immunoprecipitation-H00022943-M11-img0010.jpg)
![ELISA: Dkk-1 Antibody (2A5) [H00022943-M11] - Detection limit for recombinant GST tagged DKK1 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Dkk-1-Antibody-2A5-ELISA-H00022943-M11-img0013.jpg)
![Western Blot: OCIL/CLEC2d Antibody (4C7) [H00029121-M01] - Analysis of CLEC2D expression in transfected 293T cell line by CLEC2D monoclonal antibody (M01), clone 4C7.Lane 1: CLEC2D transfected lysate(21.8 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/OCIL-CLEC2d-Antibody-4C7-Western-Blot-H00029121-M01-img0005.jpg "Western Blot: OCIL/CLEC2d Antibody (4C7) [H00029121-M01] - Analysis of CLEC2D expression in transfected 293T cell line by CLEC2D monoclonal antibody (M01), clone 4C7.Lane 1: CLEC2D transfected lysate(21.8 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: OCIL/CLEC2d Antibody (4C7) [H00029121-M01] - Analysis of monoclonal antibody to CLEC2D on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/OCIL-CLEC2d-Antibody-4C7-Immunohistochemistry-Paraffin-H00029121-M01-img0003.jpg)
![ELISA: OCIL/CLEC2d Antibody (4C7) [H00029121-M01] - Detection limit for recombinant GST tagged CLEC2D is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/OCIL-CLEC2d-Antibody-4C7-ELISA-H00029121-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - N87 human gastric tumor xenograft immunostained with isotope control antibody (left panel) or 1:200 dilution of Integrin alpha 5 beta 1 antibody (right panel). Arrows indicate DAB immunoreactivity. Red scale bar = 10 microns. Image from verified customer review.](http://images.novusbio.com/fullsize/Integrin-alpha-5-beta-1-Antibody-M200-Volociximab-Chimeric-Immunohistochemistry-Paraffin-NBP2-52680-img0003.jpg "Immunohistochemistry-Paraffin: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - N87 human gastric tumor xenograft immunostained with isotope control antibody (left panel) or 1:200 dilution of Integrin alpha 5 beta 1 antibody (right panel). Arrows indicate DAB immunoreactivity. Red scale bar = 10 microns. Image from verified customer review.")
![Flow Cytometry: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - Flow-cytometry using the anti-alpha 5 beta 1 Integrin M200 (Volociximab). PBMC's (A) and U93 cells (B) were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of M200 (Volociximab) (NBP2-52680, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Integrin-alpha-5-beta-1-Antibody-M200-Volociximab-Chimeric-Flow-Cytometry-NBP2-52680-img0002.jpg)
![Immunofluorescence: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - Immunofluoresence staining of fixed HeLa cells with anti-alpha 5 beta 1 Integrin antibody M200 (Volociximab). Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of M200 (NBP2-52680) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-52680, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Integrin-alpha-5-beta-1-Antibody-M200-Volociximab-Chimeric-Immunofluorescence-NBP2-52680-img0001.jpg)
![Flow Cytometry: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric - Azide and BSA Free [NBP2-52680] - Abundance of two potential Hla receptors & of caveolin-1 as a potential pore-stabilizing factor.A: Representative examples of immune fluorescence assays using epifluorescence microscopy (nuclei counterstained using DAPI) that were performed on 16HBE14o-, S9 or A549 cells grown on coverslips using antibodies against ADAM10, the alpha 5 beta 1 integrin or against caveolin-1. Staining appearing in red represents specific labelling of the respective proteins. Scale bars: 10 μm. B: Semi-quantitative determination of primary & secondary antibody-mediated fluorescence in suspended individual cells by flow cytometry. During flow cytometry, the fluorescence of the antibody-tagged proteins per cell was measured & the respective medians of the detected peaks were used for calculating the means ± S.D. for the biological replicates (n = 4, each). Individual means were tested for significant differences using Student’s t-test or Welch’s t-test: * = p ≤ 0.05, ** = p ≤ 0.01 or *** = p ≤ 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32470006), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-52680_rabbit-monoclonal-integrin-alpha-5-beta-1-antibody-m200-volociximab-chimeric-310202416114751.jpg)
![Western Blot: TAK1 Antibody [NB100-56363] - Western blot analysis of TAK1 in cell lysates from NIH 3T3 cells using NB100-56363 at 1 ug/ml dilution.](http://images.novusbio.com/fullsize/TAK1-Antibody-Western-Blot-NB100-56363-img0003.jpg "Western Blot: TAK1 Antibody [NB100-56363] - Western blot analysis of TAK1 in cell lysates from NIH 3T3 cells using NB100-56363 at 1 ug/ml dilution.")
![Immunocytochemistry/Immunofluorescence: TAK1 Antibody [NB100-56363] - RH30 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-TAK1 Antibody NB100-56363 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/TAK1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56363-img0006.jpg)
![Immunohistochemistry-Paraffin: TAK1 Antibody [NB100-56363] - Analysis of a FFPE tissue section of human stomach using 1:200 dilution of TAK1 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/TAK1-Antibody-Immunohistochemistry-Paraffin-NB100-56363-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: TAK1 Antibody [NB100-56363] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-TAK1 Antibody NB100-56363 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/TAK1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56363-img0005.jpg)
![Western Blot: B7-H3/CD276 Antibody [NBP1-88966] - Western blot shows lysates of U2OS human bone osteosarcoma parental cell line and B7-H3 knockout (KO) U2OS cell line. PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human B7-H3 Polyclonal Antibody (Catalog # NBP1-88966) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for B7-H3 at approximately 95 kDa (as indicated) in the parental U2OS cell line, but is not detectable in the knockout U2OS cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Knockout-Validated-NBP1-88966-img0016.jpg "<b>Genetic Strategies Validation. </b>Western Blot: B7-H3/CD276 Antibody [NBP1-88966] - Western blot shows lysates of U2OS human bone osteosarcoma parental cell line and B7-H3 knockout (KO) U2OS cell line. PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human B7-H3 Polyclonal Antibody (Catalog # NBP1-88966) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for B7-H3 at approximately 95 kDa (as indicated) in the parental U2OS cell line, but is not detectable in the knockout U2OS cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: B7-H3/CD276 Antibody [NBP1-88966] - Staining of human cell line A-431 shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88966-img0006.jpg)
![Western Blot: B7-H3/CD276 Antibody [NBP1-88966] - Analysis in control (vector only transfected HEK293T lysate) and CD276 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Western-Blot-NBP1-88966-img0017.jpg)
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88966] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunohistochemistry-Paraffin-NBP1-88966-img0014.jpg)
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88966] - Staining of human endometrium shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-88966_rabbit-polyclonal-b7-h3-cd276-antibody-immunohistochemistry-paraffin-122202411124..jpg)
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88966] -Staining of human rectum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-88966_rabbit-polyclonal-b7-h3-cd276-antibody-immunohistochemistry-paraffin-122202411220..jpg)
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88966] -Staining of human cerebellum shows negative cytoplasmic positivity in Purkinje cells as expected.](http://images.novusbio.com/fullsize/antibody/nbp1-88966_rabbit-polyclonal-b7-h3-cd276-antibody-immunohistochemistry-paraffin-122202411310..jpg)
![Western Blot: Integrin alpha 5/CD49e Antibody (8F2O6) [NBP3-15645] - Analysis of extracts of various cell lines, using Integrin alpha 5/CD49e (ITGA5/CD49e) antibody (NBP3-15645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-8F2O6-Western-Blot-NBP3-15645-img0004.jpg "Western Blot: Integrin alpha 5/CD49e Antibody (8F2O6) [NBP3-15645] - Analysis of extracts of various cell lines, using Integrin alpha 5/CD49e (ITGA5/CD49e) antibody (NBP3-15645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: Integrin alpha 5/CD49e Antibody (8F2O6) [NBP3-15645] - Analysis of extracts of A-549 cells, using Integrin alpha 5/CD49e (ITGA5/CD49e) antibody (NBP3-15645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-8F2O6-Western-Blot-NBP3-15645-img0002.jpg)
![Western Blot: Integrin alpha 5/CD49e Antibody (8F2O6) [NBP3-15645] - Analysis of extracts of various cell lines, using Integrin alpha 5/CD49e (ITGA5/CD49e) antibody (NBP3-15645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-8F2O6-Western-Blot-NBP3-15645-img0003.jpg)
![Flow Cytometry: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Flow cytometry:1X10^6 Daudi cells (negative control,left) and K-562 cells were intracellularly-stained with Integrin alpha 5/CD49e Rabbit mAb or Rabbit IgG isotype control ,followed by Alexa Fluor 647 conjugated goat anti-rabbit pAb(1:600 dilution) staining. Non-fluorescently stained cells were used as blank control .](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-52202517303123.jpg)
![Immunohistochemistry: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Integrin alpha 5/CD49e Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-52202517352792.jpg)
![Immunohistochemistry: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Integrin alpha 5/CD49e Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-5220251734518.jpg)
![Immunocytochemistry/ Immunofluorescence: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Confocal imaging of paraffin-embedded Human placenta using Integrin alpha 5/CD49e Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-5220251734594.jpg)
![Immunohistochemistry: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Integrin alpha 5/CD49e Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-52202517363728.jpg)
![Immunohistochemistry: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using Integrin alpha 5/CD49e Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-52202517344298.jpg)
![Immunocytochemistry/ Immunofluorescence: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Confocal imaging of paraffin-embedded Rat large intestine using Integrin alpha 5/CD49e Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-5220251733528.jpg)
![Immunohistochemistry: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using Integrin alpha 5/CD49e Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-52202517363712.jpg)
![Flow Cytometry: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Flow cytometry:1X10^6 Daudi cells (negative control,left) and U-87MG cells were intracellularly-stained with Integrin alpha 5/CD49e Rabbit mAb or Rabbit IgG isotype control ,followed by Alexa Fluor 647 conjugated goat anti-rabbit pAb(1:600 dilution) staining. Non-fluorescently stained cells were used as blank control .](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-5220251733588.jpg)
![Immunohistochemistry: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using Integrin alpha 5/CD49e Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-52202517333885.jpg)
![Western Blot: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Western blot analysis of Integrin alpha 5/CD49e on NIH/3T3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-JJ08-94-Western-Blot-NBP2-67883-img0009.jpg "Western Blot: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Western blot analysis of Integrin alpha 5/CD49e on NIH/3T3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2")
![Immunocytochemistry/Immunofluorescence: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Staining Integrin alpha 5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-JJ08-94-Immunocytochemistry-Immunofluorescence-NBP2-67883-img0003.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Analysis of paraffin-embedded mouse uterus tissue using anti-Integrin alpha 5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-JJ08-94-Immunohistochemistry-Paraffin-NBP2-67883-img0007.jpg)
![Flow Cytometry: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Analysis of Jurkat cells with Integrin alpha 5 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-JJ08-94-Flow-Cytometry-NBP2-67883-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Staining Integrin alpha 5 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-JJ08-94-Immunocytochemistry-Immunofluorescence-NBP2-67883-img0002.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Analysis of paraffin-embedded human kidney tissue using anti-Integrin alpha 5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-JJ08-94-Immunohistochemistry-Paraffin-NBP2-67883-img0004.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Analysis of paraffin-embedded human tonsil tissue using anti-Integrin alpha 5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-JJ08-94-Immunohistochemistry-Paraffin-NBP2-67883-img0005.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Analysis of paraffin-embedded human uterus tissue using anti-Integrin alpha 5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-JJ08-94-Immunohistochemistry-Paraffin-NBP2-67883-img0006.jpg)
![Western Blot: TAK1 Antibody (JM73-19) [NBP2-67180] - Western blot analysis of TAK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysate Lane 2: A431 cell lysate](http://images.novusbio.com/fullsize/TAK1-Antibody-JM73-19-Western-Blot-NBP2-67180-img0008.jpg "Western Blot: TAK1 Antibody (JM73-19) [NBP2-67180] - Western blot analysis of TAK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysate Lane 2: A431 cell lysate")
![Immunocytochemistry/Immunofluorescence: TAK1 Antibody (JM73-19) [NBP2-67180] - Staining TAK1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TAK1-Antibody-JM73-19-Immunocytochemistry-Immunofluorescence-NBP2-67180-img0007.jpg)
![Immunohistochemistry-Paraffin: TAK1 Antibody (JM73-19) [NBP2-67180] - Analysis of paraffin-embedded mouse stomach tissue using anti-TAK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TAK1-Antibody-JM73-19-Immunohistochemistry-Paraffin-NBP2-67180-img0005.jpg)
![Flow Cytometry: TAK1 Antibody (JM73-19) [NBP2-67180] - Analysis of A431 cells with TAK1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/TAK1-Antibody-JM73-19-Flow-Cytometry-NBP2-67180-img0001.jpg)
![Western Blot: TAK1 Antibody (JM73-19) [NBP2-67180] - Analysis of TAK1 on human placenta tissue lysate using anti-TAK1 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/TAK1-Antibody-JM73-19-Western-Blot-NBP2-67180-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: TAK1 Antibody (JM73-19) [NBP2-67180] - Staining TAK1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TAK1-Antibody-JM73-19-Immunocytochemistry-Immunofluorescence-NBP2-67180-img0002.jpg)
![Immunohistochemistry-Paraffin: TAK1 Antibody (JM73-19) [NBP2-67180] - Analysis of paraffin-embedded human placenta tissue using anti-TAK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TAK1-Antibody-JM73-19-Immunohistochemistry-Paraffin-NBP2-67180-img0003.jpg)
![Immunohistochemistry-Paraffin: TAK1 Antibody (JM73-19) [NBP2-67180] - Analysis of paraffin-embedded human prostate tissue using anti-TAK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TAK1-Antibody-JM73-19-Immunohistochemistry-Paraffin-NBP2-67180-img0004.jpg)
![Western Blot: SMURF2 Antibody [NBP2-57554] - Analysis in human cell line U-251 MG.](http://images.novusbio.com/fullsize/SMURF2-Antibody-Western-Blot-NBP2-57554-img0008.jpg "Western Blot: SMURF2 Antibody [NBP2-57554] - Analysis in human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: SMURF2 Antibody [NBP2-57554] - Staining of human cell line BJ shows localization to nuclear speckles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SMURF2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57554-img0001.jpg)
![Immunohistochemistry-Paraffin: SMURF2 Antibody [NBP2-57554] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/SMURF2-Antibody-Immunohistochemistry-Paraffin-NBP2-57554-img0007.jpg)
![Immunohistochemistry-Paraffin: SMURF2 Antibody [NBP2-57554] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/SMURF2-Antibody-Immunohistochemistry-Paraffin-NBP2-57554-img0004.jpg)
![Immunohistochemistry-Paraffin: SMURF2 Antibody [NBP2-57554] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/SMURF2-Antibody-Immunohistochemistry-Paraffin-NBP2-57554-img0005.jpg)
![Immunohistochemistry-Paraffin: SMURF2 Antibody [NBP2-57554] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/SMURF2-Antibody-Immunohistochemistry-Paraffin-NBP2-57554-img0006.jpg)
![Western Blot: B7-H3/CD276 Antibody [NBP3-14669] - Samples: Whole cell lysate (50 ug) from TCMK-1, NIH 3T3,AlphaTC1 Clone 9, 3T3-L1, and CT26 cells prepared usingNETN lysis buffer. Antibody: Rabbit anti-B7-H3/CD276recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goatanti-rabbit IgG. Chemiluminescence with anexposure time of 3 minutes.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Western-Blot-NBP3-14669-img0002.jpg "Western Blot: B7-H3/CD276 Antibody [NBP3-14669] - Samples: Whole cell lysate (50 ug) from TCMK-1, NIH 3T3,AlphaTC1 Clone 9, 3T3-L1, and CT26 cells prepared usingNETN lysis buffer. Antibody: Rabbit anti-B7-H3/CD276recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goatanti-rabbit IgG. Chemiluminescence with anexposure time of 3 minutes.")
![Immunocytochemistry/Immunofluorescence: B7-H3/CD276 Antibody (BLR025F) [NBP3-14669] - Section of A-172 cells. Antibody: Rabbit anti-B7-H3/CD276 recombinant monoclonal used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunohistochemistry-NBP3-14669-img0005.jpg)
![Immunohistochemistry: B7-H3/CD276 Antibody [NBP3-14669] - Sample: FFPE section of humanlung carcinoma. Antibody: Rabbit anti-B7-H3/CD276recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat antirabbit IgG. Substrate: Opal™. Counterstain:DAPI (blue).](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunohistochemistry-NBP3-14669-img0006.jpg)
![Western Blot: B7-H3/CD276 Antibody [NBP3-14669] - Rabbit anti CD276 recombinant monoclonal antibody (NBP3-14669 lot 1) used at 1:1000. HRP-conjugated goat anti-rabbit IgG (NB7156). Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-GAPDH (NB300-322).](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Western-Blot-NBP3-14669-img0001.jpg)
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody (BLR025F) [NBP3-14669] - Section of ovarian carcinoma. Antibody: Rabbit anti-B7-H3/CD276 recombinant monoclonal antibody used at 1:200. Secondary: HRP-conjugated goat antirabbit IgG (A120-501P). Substrate: DAB.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunohistochemistry-NBP3-14669-img0004.jpg)
![Immunoprecipitation: B7-H3/CD276 Antibody [NBP3-14669] - Samples: Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from HeLa cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-B7-H3/CD276 recombinant monoclonal antibodyused for IP at 3 ul per reaction. B7-H3/CD276 was also immunoprecipitated by rabbit anti-B7-H3/CD276 recombinant monoclonal antibody. For blotting immunoprecipitated B7-H3/CD27 was used at 1:1000.Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunoprecipitation-NBP3-14669-img0003.jpg)
![B7-H3/CD276 Antibody (BLR025F) [NBP3-14669] - Detection of human B7-H3/CD276 (shaded) in 293T cells by flow cytometry. Antibody: Rabbit antiB7-H3/CD276 recombinant monoclonal [BLR025F]or isotype control (unshaded).Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-BLR025F-NBP3-14669-img0010.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody [NBP1-84576] - Staining in human placenta and skeletal muscle tissues using anti-ITGA5 antibody. Corresponding ITGA5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-Immunohistochemistry-Paraffin-NBP1-84576-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody [NBP1-84576] - Staining in human placenta and skeletal muscle tissues using anti-ITGA5 antibody. Corresponding ITGA5 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody [NBP1-84576] - Staining of human endometrium shows weak to moderate membranous positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-Immunohistochemistry-Paraffin-NBP1-84576-img0014.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody [NBP1-84576] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-Immunohistochemistry-Paraffin-NBP1-84576-img0010.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody [NBP1-84576] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-Immunohistochemistry-Paraffin-NBP1-84576-img0011.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody [NBP1-84576] - Staining of human prostate shows moderate membranous positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-Immunohistochemistry-Paraffin-NBP1-84576-img0013.jpg)
![Western Blot: IL-11R alpha Antibody (10Z3S9) [NBP3-16809] - Western blot analysis of extracts of various cell lines, using (NBP3-16809) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/IL-11R-alpha-Antibody-10Z3S9-Western-Blot-NBP3-16809-img0008.jpg "Western Blot: IL-11R alpha Antibody (10Z3S9) [NBP3-16809] - Western blot analysis of extracts of various cell lines, using (NBP3-16809) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: IL-11R alpha Antibody (10Z3S9) [NBP3-16809] - Western blot analysis of extracts of Rat brain, using (NBP3-16809) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/IL-11R-alpha-Antibody-10Z3S9-Western-Blot-NBP3-16809-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-11R alpha Antibody (10Z3S9) [NBP3-16809] - Confocal imaging of K-562 cells using IL-11R alpha Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16809_rabbit-il-11r-alpha-mab-10z3s9-8220252010486.jpg)
![Immunohistochemistry: IL-11R alpha Antibody (10Z3S9) [NBP3-16809] - Immunohistochemistry analysis of IL-11R alpha in paraffin-embedded human colon tissue using IL-11R alpha Rabbit mAb at a dilution of 1:400 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16809_rabbit-il-11r-alpha-mab-10z3s9-822025209878.jpg)
![Immunohistochemistry: IL-11R alpha Antibody (10Z3S9) [NBP3-16809] - Immunohistochemistry analysis of IL-11R alpha in paraffin-embedded mouse brain tissue using IL-11R alpha Rabbit mAb at a dilution of 1:400 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16809_rabbit-il-11r-alpha-mab-10z3s9-822025208446.jpg)
![Immunohistochemistry: IL-11R alpha Antibody (10Z3S9) [NBP3-16809] - Immunohistochemistry analysis of IL-11R alpha in paraffin-embedded rat spleen tissue using IL-11R alpha Rabbit mAb at a dilution of 1:400 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16809_rabbit-il-11r-alpha-mab-10z3s9-8220252010421.jpg)
![Immunohistochemistry: IL-11R alpha Antibody (10Z3S9) [NBP3-16809] - Immunohistochemistry analysis of IL-11R alpha in paraffin-embedded human liver cancer tissue using IL-11R alpha Rabbit mAb at a dilution of 1:400 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16809_rabbit-il-11r-alpha-mab-10z3s9-822025209813.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-11R alpha Antibody (10Z3S9) [NBP3-16809] - Confocal imaging of RAW 264.7 cells using IL-11R alpha Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16809_rabbit-il-11r-alpha-mab-10z3s9-822025209837.jpg)