| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76701SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-44521 | |||
| FITC | NB110-60015 | |||
| HRP | NBP1-44520 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47704-0.1mg | |||
| IHC-Prediluted | NBP2-48345 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87482-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56421SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70588 | |||
![Western Blot: TIM-1/KIM-1/HAVCR Antibody - BSA Free [NBP1-76701] - TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - In vitro nephrotoxicity assessment of kidney spheroids. Western blotting analysis of kidney injury markers in kidney spheroids after treatment with 20 uM cisplatin (CPT) or 50 uM cisplatin cyclosporin A (CSA). All data are shown as the means +/- s.e.m. *p < 0.05 compared with control by unpaired Student's t-test. Scale bar, 20 um. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-019-53855-2) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TIM-1-KIM-1-HAVCR-Antibody-Western-Blot-NBP1-76701-img0016.jpg "<b>Biological Strategies Validation. </b>Western Blot: TIM-1/KIM-1/HAVCR Antibody - BSA Free [NBP1-76701] - TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - In vitro nephrotoxicity assessment of kidney spheroids. Western blotting analysis of kidney injury markers in kidney spheroids after treatment with 20 uM cisplatin (CPT) or 50 uM cisplatin cyclosporin A (CSA). All data are shown as the means +/- s.e.m. *p < 0.05 compared with control by unpaired Student's t-test. Scale bar, 20 um. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-019-53855-2) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: TIM-1/KIM-1/HAVCR Antibody - BSA Free [NBP1-76701] - TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - Analysis of human uterus tissue using anti-TIM-1 antibody (3809) at 10 ug/ml.Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C. A goat anti-rabbit IgG H&L (HRP)at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/TIM-1-KIM-1-HAVCR-Antibody-Immunohistochemistry-Paraffin-NBP1-76701-img0013.jpg)
![Western Blot: TIM-1/KIM-1/HAVCR Antibody - BSA Free [NBP1-76701] - TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - Loading: 15 ug of NIH/3T3 cell lysates. Antibodies: TIM-1 3809 (8 ug/mL), overnight incubation at 4 C in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/TIM-1-KIM-1-HAVCR-Antibody-Western-Blot-NBP1-76701-img0010.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: TIM-1/KIM-1/HAVCR Antibody - BSA Free [NBP1-76701] - TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - Loading: 15 ug of lysates per lane. Antibodies: TIM-1 3809 (8 ug/mL), TIM-13811 (1 ug/mL), beta-actin (1 ug/mL), and GAPDH (0.02 ug/mL), overnightincubation at 4C (3809) or 1h incubation at RT in 5% NFDM/TBST. Secondary:Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/TIM-1-KIM-1-HAVCR-Antibody-Western-Blot-NBP1-76701-img0012.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TIM-1/KIM-1/HAVCR Antibody - BSA Free [NBP1-76701] - TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - HeLa cells were transfected with control siRNAs (lane 1) or TIM-1 siRNAs (lane 2) Loading: 15 ug of HeLa whole cell lysates per lane. Antibodies: 3809 (8 ug/mL), 1 hincubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/TIM-1-KIM-1-HAVCR-Antibody-Western-Blot-NBP1-76701-img0015.jpg)
![Western Blot: TIM-1/KIM-1/HAVCR Antibody - BSA Free [NBP1-76701] - Figure 1 Western Blot Validation in Human Cell LinesLoading: 15 u of lysates per lane.Antibodies: TIM-1 (8 u/mL), overnight incubation at 4; C in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76701_rabbit-polyclonal-tim-1-kim-1-havcr-antibody-2711202420171718.gif)
![Immunocytochemistry/ Immunofluorescence: TIM-1/KIM-1/HAVCR Antibody - BSA Free [NBP1-76701] - Validation of TIM-1/KIM-1/HAVCR in Human Uterus Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed human uterus cells labeling TIM-1/KIM-1/HAVCR with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on human uterus cells.](http://images.novusbio.com/fullsize/nbp1-76701_rabbit-polyclonal-tim-1-kim-1-havcr-antibody-271120241937768.jpg)
![Western Blot: nNOS Antibody [NBP1-39681] - Effects of ER alpha and ER beta Antagonists on NE Regulation of VMN nNOS Protein Expression. Micropunch-dissected VMN tissue was obtained from groups of ovariectomized, estradiol-replaced female rats pretreated by intra-VMN delivery of MPP, PHTPP, or vehicle (Veh) prior to NE administration for Western blot analysis of neuronal nitric oxide synthase (nNOS) - Panel 2A,F(5, 12)=12.67,p<.0001 - protein expression. Data depict mean normalized protein optical density (O.D.) values+/-SEMfor groups of rats (n=6 per group) infused with Veh (white bars) or NE (gray bars) after delivery of Veh (solid bars), MPP (diagonal-striped bars), or PHTPP (cross-hatched bars) administration. *p<.05; **p<.01; ***p<.001. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32233668/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nNOS-Antibody-Western-Blot-NBP1-39681-img0007.jpg "Western Blot: nNOS Antibody [NBP1-39681] - Effects of ER alpha and ER beta Antagonists on NE Regulation of VMN nNOS Protein Expression. Micropunch-dissected VMN tissue was obtained from groups of ovariectomized, estradiol-replaced female rats pretreated by intra-VMN delivery of MPP, PHTPP, or vehicle (Veh) prior to NE administration for Western blot analysis of neuronal nitric oxide synthase (nNOS) - Panel 2A,F(5, 12)=12.67,p<.0001 - protein expression. Data depict mean normalized protein optical density (O.D.) values+/-SEMfor groups of rats (n=6 per group) infused with Veh (white bars) or NE (gray bars) after delivery of Veh (solid bars), MPP (diagonal-striped bars), or PHTPP (cross-hatched bars) administration. *p<.05; **p<.01; ***p<.001. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32233668/) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: nNOS Antibody [NBP1-39681] - Analysis of anti-nNOS / NOS1 antibody with human brain, cerebellum at concentration 5 ug/ml.](http://images.novusbio.com/fullsize/nNOS-Antibody-Immunohistochemistry-Paraffin-NBP1-39681-img0004.jpg)
![Western Blot: nNOS Antibody [NBP1-39681]](http://images.novusbio.com/fullsize/nNOS-Antibody-Western-Blot-NBP1-39681-img0006.jpg)
![Immunohistochemistry-Paraffin: nNOS Antibody [NBP1-39681] - Analysis of anti-nNOS / NOS1 antibody with human brain, cortex at concentration 5 ug/ml.](http://images.novusbio.com/fullsize/nNOS-Antibody-Immunohistochemistry-Paraffin-NBP1-39681-img0003.jpg)
![Western Blot: nNOS Antibody [NBP1-39681] - Laser-Catapult Microdissection of Immunolabeled ventromedial hypothalamic nucleus (VMN) GABA or Nitrergic Neurons: Western Blot Confirmation of Accuracy of Immunocytochemical Identification of Neurotransmitter Phenotype. VMN neurons were identified in situ for glutamate decarboxylase65/67 (GAD65/67)- (top row, Panel 1A) or neuronal nitric oxide (nNOS)-immunoreactivity (-ir) (bottom row, Panel 2A); representative GAD65/67- or nNOS-ir-positive neurons are indicated by blue arrows. Areas shown in Panels 1A & 2A were rephotographed after positioning of a continuous laser track (depicted in green) around a single GAD65/67-ir (Panel 1B, blue arrow) or nNOS-ir neuron (Panel 2B, blue arrow) & subsequent ejection of the encircled cell by laser pulse (Panels 1C & 2C). Note that this microdissection technique causes negligible destruction of surrounding tissue & minimal inclusion of adjacent tissue. Western blot analysis of triplicate cell lysate pools produced from immunolabeled GAD65/67 (Panel 1D) or nNOS (Panel 2D) neurons from Veh/Veh animals showed that these proteins are expressed in VMN neuron samples obtained by combinatory immunocytochemistry/laser-catapult microdissection. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32233668), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-39681_rabbit-nnos-pab-310202416154636.jpg)
![Western Blot: nNOS Antibody [NBP1-39681] - Effects of ER alpha & ER beta Antagonists on NE Regulation of VMN GAD & nNOS Protein Expression. Micropunch-dissected VMN tissue was obtained from groups of ovariectomized, estradiol-replaced female rats pretreated by intra-VMN delivery of MPP, PHTPP, or vehicle (Veh) prior to NE administration for Western blot analysis of neuronal nitric oxide synthase (nNOS)—Panel 2A, F(5, 12) = 12.67, p < .0001—or glutamate decarboxylase65/67 (GAD)—Panel 2B, F(5, 12) = 7.13, p = .0003—protein expression. Data depict mean normalized protein optical density (O.D.) values ± SEM for groups of rats (n = 6 per group) infused with Veh (white bars) or NE (gray bars) after delivery of Veh (solid bars), MPP (diagonal-striped bars), or PHTPP (cross-hatched bars) administration. *p < .05; **p < .01; ***p < .001. MPP = 1,3-Bis(4-hydroxyphenyl)-4-methyl-5-[4-(2-piperidinylethoxy)phenol]-1H-pyrazole dihydrochloride; PHTPP = 4-[2-phenyl-5,7-bis(trifluoromethyl)pyrazolo[1,5-a]pyrimidin-3-yl]phenol; NE = norepinephrine. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32233668), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-39681_rabbit-nnos-pab-310202416175279.jpg)
![Immunocytochemistry/Immunofluorescence: nNOS Antibody [NB100-858] - Action potentials were evoked with depolarizing current pulses. (A) Neuron from colonic specimen of non-treated patient fired 1 action potential in response to a depolarizing current. (A') Intracellular injection of carboxyfluorescein during recording confirmed Dogiel type I, uniaxonal morphology. (A](http://images.novusbio.com/fullsize/nNOS-Antibody-Immunocytochemistry-Immunofluorescence-NB100-858-img0005.jpg "Immunocytochemistry/Immunofluorescence: nNOS Antibody [NB100-858] - Action potentials were evoked with depolarizing current pulses. (A) Neuron from colonic specimen of non-treated patient fired 1 action potential in response to a depolarizing current. (A') Intracellular injection of carboxyfluorescein during recording confirmed Dogiel type I, uniaxonal morphology. (A")
![Immunohistochemistry-Paraffin: nNOS Antibody [NB100-858] - Staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nNOS-Antibody-Immunohistochemistry-Paraffin-NB100-858-img0006.jpg)
![Flow Cytometry: nNOS Antibody [NB100-858] - Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nNOS-Antibody-Flow-Cytometry-NB100-858-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: nNOS Antibody [NB100-858] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane, cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/nNOS-Antibody-Immunocytochemistry-Immunofluorescence-NB100-858-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: nNOS Antibody [NB100-858] - Wholemount preparations of the myenteric neurons in the distal colon segments following 3 (2a-2d) and 7 (4a-4d) days of repeated in vivo oxaliplatin administration compared to sham-treated animals at 3 (1a-1d) and 7 (3a-3d) days. Myenteric ganglia and neurons labeled with beta-Tubulin Tuj1 (red), nuclei labeled with DAPI (blue) (arrows) can be seen within the ganglion (1b). Smooth muscle cells (asterisks) are also labeled with DAPI (1b). Significant increase in the proportion of nNOS neurons (green) can be seen at both Days 3 and 7 after oxaliplatin treatment. Scale bar = 20 um. Image collected and cropped by CiteAb from the following publication (www.journal.frontiersin.org/article/10.3389/fnins.2013.00030/abstract) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nNOS-Antibody-Immunocytochemistry-Immunofluorescence-NB100-858-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: nNOS Antibody [NB100-858] - (A) Wholemount preparations of the myenteric neurons in the distal colon segments following 3 (2a–2d) & 7 (4a–4d) days of repeated in vivo oxaliplatin administration compared to sham-treated animals at 3 (1a–1d) & 7 (3a–3d) days. Myenteric ganglia & neurons labeled with beta -Tubulin Tuj1 (red), nuclei labeled with DAPI (blue) (arrows) can be seen within the ganglion (1b). Smooth muscle cells (asterisks) are also labeled with DAPI (1b). Significant increase in the proportion of NOS neurons (green) can be seen at both Days 3 & 7 after oxaliplatin treatment. Scale bar = 20 μm. (B) Wholemount preparations of the myenteric neurons in the distal colon segments following 14 (2a–2d) & 21 (4a–4d) days of repeated in vivo oxaliplatin administration compared to sham-treated animals at 14 (1a–1d) & 21 (3a–3d) days. The reduction in total number of neurons & increase in the proportion of NOS neurons was found at both Days 14 & 21 after oxaliplatin treatment. Scale bar = 20 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23486839), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-858_goat-polyclonal-nnos-antibody-310202416284925.jpg)
![Immunocytochemistry/ Immunofluorescence: nNOS Antibody [NB100-858] - (A) Wholemount preparations of the myenteric neurons in the distal colon segments following 3 (2a–2d) & 7 (4a–4d) days of repeated in vivo oxaliplatin administration compared to sham-treated animals at 3 (1a–1d) & 7 (3a–3d) days. Myenteric ganglia & neurons labeled with beta -Tubulin Tuj1 (red), nuclei labeled with DAPI (blue) (arrows) can be seen within the ganglion (1b). Smooth muscle cells (asterisks) are also labeled with DAPI (1b). Significant increase in the proportion of NOS neurons (green) can be seen at both Days 3 & 7 after oxaliplatin treatment. Scale bar = 20 μm. (B) Wholemount preparations of the myenteric neurons in the distal colon segments following 14 (2a–2d) & 21 (4a–4d) days of repeated in vivo oxaliplatin administration compared to sham-treated animals at 14 (1a–1d) & 21 (3a–3d) days. The reduction in total number of neurons & increase in the proportion of NOS neurons was found at both Days 14 & 21 after oxaliplatin treatment. Scale bar = 20 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23486839), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-858_goat-polyclonal-nnos-antibody-310202416284920.jpg)
![Western Blot: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Analysis using the FITC conjugate of NB500-317. Detection (non-reducing conditions) of whole cell lysate of various cell lines Lane 1: RAJI human Burkitt lymphoma cell line Lane 2: EL4 mouse lymphoblastic lymphoma cell line Lane 3: U937 human histiocytic lymphoma cell line.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-01-Western-Blot-NB500-317-img0003.jpg "Western Blot: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Analysis using the FITC conjugate of NB500-317. Detection (non-reducing conditions) of whole cell lysate of various cell lines Lane 1: RAJI human Burkitt lymphoma cell line Lane 2: EL4 mouse lymphoblastic lymphoma cell line Lane 3: U937 human histiocytic lymphoma cell line.")
![Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Confocal microscopy of human beta2-microglobulin-transfected canine cells labeled A) with B2M-01 antibody Alexa Fluor (R) 647 conjugate and B) with Alexa Fluor (R) 647 conjugated Mouse IgG2a Isotype Control. Cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Tween20 containing 2% BSA. Antibody concentration 2ug/ml.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-01-Immunocytochemistry-Immunofluorescence-NB500-317-img0005.jpg)
![Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Staining of beta-2-microglobulin in human peripheral blood with anti-beta-2-microglobulin (B2M-01) biotin.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-01-Flow-Cytometry-NB500-317-img0012.jpg)
![Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Surface staining of beta2-microglobulin in human peripheral blood with anti-beta2-microglobulin (B2M-01) FITC.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-01-Flow-Cytometry-NB500-317-img0008.jpg)
![Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Surface staining of beta2-microglobulin in human peripheral blood with anti-beta2-microglobulin (B2M-01) PerCP-CyTM5.5.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-01-Flow-Cytometry-NB500-317-img0010.jpg)
![Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Surface staining of beta2-microglobulin in human peripheral blood with anti-beta2-microglobulin (B2M-01) PE.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-01-Flow-Cytometry-NB500-317-img0011.jpg)
![ELISA: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Analysis of HLA-G and beta2-microglobulin in supernatant of HLA-G transfectants with anti-beta2-microglobulin (B2M-01) HRP as the detection antibody. Image using the HRP format of this antibody.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-01-ELISA-NB500-317-img0013.jpg)
![Immunohistochemistry-Paraffin: DPPIV/CD26 Antibody - BSA Free [NB100-59021] - Immunohistochemical expression of DPPIV/CD26 in colorectal adenocarcinoma. DPPIV/CD26 immunostaining is located in the cytoplasm of adenomas and CRC cells displaying luminal membrane reinforcement. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0119436), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DPPIV-CD26-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB100-59021-img0005.jpg "Immunohistochemistry-Paraffin: DPPIV/CD26 Antibody - BSA Free [NB100-59021] - Immunohistochemical expression of DPPIV/CD26 in colorectal adenocarcinoma. DPPIV/CD26 immunostaining is located in the cytoplasm of adenomas and CRC cells displaying luminal membrane reinforcement. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0119436), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: DPPIV/CD26 Antibody - BSA Free [NB100-59021] - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)](http://images.novusbio.com/fullsize/DPPIV-CD26-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB100-59021-img0002.jpg)
![Immunohistochemistry-Paraffin: DPPIV/CD26 Antibody - BSA Free [NB100-59021] - Immunohistochemical expression of DPPIV/CD26 in normal colorectal mucosa. DPPIV/CD26 immunostaining is located in the cytoplasm of adenomas and CRC cells displaying luminal membrane reinforcement. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0119436), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DPPIV-CD26-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB100-59021-img0003.jpg)
![Immunohistochemistry-Paraffin: DPPIV/CD26 Antibody - BSA Free [NB100-59021] - Immunohistochemical expression of DPPIV/CD26 in tubulovillous colonic adenoma. DPPIV/CD26 immunostaining is located in the cytoplasm of adenomas and CRC cells displaying luminal membrane reinforcement. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0119436), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DPPIV-CD26-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB100-59021-img0004.jpg)
![Immunohistochemistry-Paraffin: DPPIV/CD26 Antibody - BSA Free [NB100-59021] - Immunohistochemical analysis of human prostate.](http://images.novusbio.com/fullsize/DPPIV-CD26-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB100-59021-img0006.jpg)
![Western Blot: beta 2-Microglobulin Antibody [NBP2-15566] - Lane 1: HEK cells. Lane 2: HEK cells with siRNA against beta 2-Microglobulin antibody. Lane 3: HEK cells stimulated for increased beta 2-Microglobulin expression. Image from verified customer review.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-Western-Blot-NBP2-15566-img0015.jpg "Western Blot: beta 2-Microglobulin Antibody [NBP2-15566] - Lane 1: HEK cells. Lane 2: HEK cells with siRNA against beta 2-Microglobulin antibody. Lane 3: HEK cells stimulated for increased beta 2-Microglobulin expression. Image from verified customer review.")
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP2-15566] - Mouse spleen. B2M stained by Beta 2 Microglobulin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-Immunohistochemistry-Paraffin-NBP2-15566-img0016.jpg)
![Western Blot: beta 2-Microglobulin Antibody [NBP2-15566] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody diluted at 1:500.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-Western-Blot-NBP2-15566-img0008.jpg)
![Western Blot: beta 2-Microglobulin Antibody [NBP2-15566] - Mouse plasma (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody diluted at 1:1500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-Western-Blot-NBP2-15566-img0012.jpg)
![Western Blot: beta 2-Microglobulin Antibody [NBP2-15566] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with beta 2-Microglobulin antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-Western-Blot-NBP2-15566-img0014.jpg)
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP2-15566] - Cal27 xenograft. beta 2 Microglobulin antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-Immunohistochemistry-Paraffin-NBP2-15566-img0005.jpg)
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP2-15566] - Human endometrial cancer. beta 2 Microglobulin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-Immunohistochemistry-Paraffin-NBP2-15566-img0007.jpg)
![Immunoprecipitation: beta 2-Microglobulin Antibody [NBP2-15566] - HeLa whole cell extract A. 30 ug HeLa whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of B2M protein by 4 ug beta 2 Microglobulin antibody 15 % SDS-PAGE The mmunoprecipitated B2M protein was detected by beta 2 Microglobulin antibody diluted at 1:500.[EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-Immunoprecipitation-NBP2-15566-img0010.jpg)
![Western Blot: beta 2-Microglobulin Antibody [NBP2-15566] - Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-15566_rabbit-polyclonal-beta-2-microglobulin-antibody-western-blot-16122024101912..png)
![Western Blot: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Western Blot Analysis of THP-1 and Raji cell lysates using beta 2-Microglobulin antibody (B2M/961).](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-961-Western-Blot-NBP2-44471-img0017.jpg "Western Blot: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Western Blot Analysis of THP-1 and Raji cell lysates using beta 2-Microglobulin antibody (B2M/961).")
![Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Immunofluorescence Analysis of HeLa cells labeling with beta 2-Microglobulin Antibody (B2M/961) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-961-Immunocytochemistry-Immunofluorescence-NBP2-44471-img0010.jpg)
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with beta 2-Microglobulin Antibody (B2M/961).](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-961-Immunohistochemistry-Paraffin-NBP2-44471-img0013.jpg)
![Flow Cytometry: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Flow Cytometric Analysis of Human HeLa cells. beta 2-Microglobulin Antibody (B2M/961) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red)](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-961-Flow-Cytometry-NBP2-44471-img0009.jpg)
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Human Bladder Carcinoma stained with Beta-2-Microglobulin Monoclonal Antibody (B2M/961)](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-961-Immunohistochemistry-Paraffin-NBP2-44471-img0001.jpg)
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Human Cervical Carcinoma stained with Beta-2-Microglobulin Monoclonal Antibody (B2M/961)](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-961-Immunohistochemistry-Paraffin-NBP2-44471-img0002.jpg)
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Human Cervical Carcinoma stained with Beta-2-Microglobulin Monoclonal Antibody (B2M/961).](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-961-Immunohistochemistry-Paraffin-NBP2-44471-img0003.jpg)
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Human Colon Carcinoma stained with Beta-2-Microglobulin Monoclonal Antibody (B2M/961)](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-961-Immunohistochemistry-Paraffin-NBP2-44471-img0004.jpg)
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Human Endometrial Carcinoma stained with Beta-2-Microglobulin Monoclonal Antibody (B2M/961)](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-961-Immunohistochemistry-Paraffin-NBP2-44471-img0005.jpg)
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Human Melanoma stained with Beta-2-Microglobulin Monoclonal Antibody (B2M/961).](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-961-Immunohistochemistry-Paraffin-NBP2-44471-img0006.jpg)
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Human Renal Carcinoma stained with Beta-2-Microglobulin Monoclonal Antibody (B2M/961)](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-961-Immunohistochemistry-Paraffin-NBP2-44471-img0007.jpg)
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with beta 2-Microglobulin Antibody (B2M/961).](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-961-Immunohistochemistry-Paraffin-NBP2-44471-img0011.jpg)
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with beta 2-Microglobulin Antibody (B2M/961).](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-961-Immunohistochemistry-Paraffin-NBP2-44471-img0012.jpg)
![Western Blot: beta 2-Microglobulin Antibody (3F9-2C2) [H00000567-M01] - Analysis of B2M expression in transfected 293T cell line by B2M monoclonal antibody (M01), clone 3F9-2C2.Lane 1: B2M transfected lysate(13.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-3F9-2C2-Western-Blot-H00000567-M01-img0015.jpg "Western Blot: beta 2-Microglobulin Antibody (3F9-2C2) [H00000567-M01] - Analysis of B2M expression in transfected 293T cell line by B2M monoclonal antibody (M01), clone 3F9-2C2.Lane 1: B2M transfected lysate(13.7 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody (3F9-2C2) [H00000567-M01] - Analysis of monoclonal antibody to B2M on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-3F9-2C2-Immunocytochemistry-Immunofluorescence-H00000567-M01-img0009.jpg)
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (3F9-2C2) [H00000567-M01] - Analysis of monoclonal antibody to B2M on formalin-fixed paraffin-embedded human esophagus. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-3F9-2C2-Immunohistochemistry-Paraffin-H00000567-M01-img0011.jpg)
![Western Blot: beta 2-Microglobulin Antibody (3F9-2C2) [H00000567-M01] - Analysis of B2M over-expressed 293 cell line, cotransfected with B2M Validated Chimera RNAi ( Cat # H00000567-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with B2M monoclonal antibody (M01), clone 3F9-2C2 (Cat # H00000567-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-3F9-2C2-Western-Blot-H00000567-M01-img0012.jpg)
![Western Blot: beta 2-Microglobulin Antibody (3F9-2C2) [H00000567-M01] - B2M monoclonal antibody (M01), clone 3F9-2C2 Analysis of B2M expression in U-2 OS.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-3F9-2C2-Western-Blot-H00000567-M01-img0014.jpg)
![Proximity Ligation Assay: beta 2-Microglobulin Antibody (3F9-2C2) [H00000567-M01] - Analysis of protein-protein interactions between CALR and B2M. HeLa cells were stained with anti-CALR rabbit purified polyclonal 1:1200 and anti-B2M mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-3F9-2C2-Proximity-Ligation-Assay-H00000567-M01-img0007.jpg)
![ELISA: beta 2-Microglobulin Antibody (3F9-2C2) [H00000567-M01] - Detection limit for recombinant GST tagged B2M is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-3F9-2C2-ELISA-H00000567-M01-img0016.jpg)
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP1-87482] - Staining in human lymph node and skeletal muscle tissues using anti-B2M antibody. Corresponding B2M RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-Immunohistochemistry-Paraffin-NBP1-87482-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP1-87482] - Staining in human lymph node and skeletal muscle tissues using anti-B2M antibody. Corresponding B2M RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: beta 2-Microglobulin Antibody [NBP1-87482] - Analysis in human cell lines HeLa and HEK293. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-Western-Blot-NBP1-87482-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody [NBP1-87482] - Staining of human cell line U-251 MG shows localization to plasma membrane, cytosol and the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87482-img0014.jpg)
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP1-87482] - Staining of human lymph node shows strong cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-Immunohistochemistry-Paraffin-NBP1-87482-img0017.jpg)
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP1-87482] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-Immunohistochemistry-Paraffin-NBP1-87482-img0012.jpg)
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP1-87482] - Staining of human cerebral cortex shows weak cytoplasmic positivity in neurons as expected.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-Immunohistochemistry-Paraffin-NBP1-87482-img0015.jpg)
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP1-87482] - Staining of human kidney shows moderate cytoplasmic positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-Immunohistochemistry-Paraffin-NBP1-87482-img0016.jpg)
![Western Blot: TIM-1/KIM-1/HAVCR Antibody [NB100-56421] - Analysis of TIM-1/KIM-1/HAVCR in human brain lysate in the A) absence and B) presence of immunizing peptide, and C) mouse brain lysate using TIM-1 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/TIM-1-KIM-1-HAVCR-Antibody-Western-Blot-NB100-56421-img0003.jpg "Western Blot: TIM-1/KIM-1/HAVCR Antibody [NB100-56421] - Analysis of TIM-1/KIM-1/HAVCR in human brain lysate in the A) absence and B) presence of immunizing peptide, and C) mouse brain lysate using TIM-1 antibody at 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: TIM-1/KIM-1/HAVCR Antibody [NB100-56421] - Caco-2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-TIM-1/KIM-1/HAVCR Antibody at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TIM-1-KIM-1-HAVCR-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56421-img0004.jpg)
![Western Blot: DPPIV/CD26 Antibody (11D7) [NBP2-02154] - Western Blot of DPP4 protein level by using anti-DPP4 antibody in mouse MLE15 cells.](http://images.novusbio.com/fullsize/DPPIV-CD26-Antibody-11D7-Western-Blot-NBP2-02154-img0011.jpg "Western Blot: DPPIV/CD26 Antibody (11D7) [NBP2-02154] - Western Blot of DPP4 protein level by using anti-DPP4 antibody in mouse MLE15 cells.")
![Immunocytochemistry/Immunofluorescence: DPPIV/CD26 Antibody (OTI11D7) [NBP2-02154] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CD26.](http://images.novusbio.com/fullsize/DPPIV-CD26-Antibody-11D7-Immunocytochemistry-Immunofluorescence-NBP2-02154-img0005.jpg)
![Immunohistochemistry: DPPIV/CD26 Antibody (11D7) [NBP2-02154] - Lungs harvested from BALB/c mice at day 3. Dilution: 1:800](http://images.novusbio.com/fullsize/DPPIV-CD26-Antibody-11D7-Immunohistochemistry-NBP2-02154-img0010.jpg)
![Flow Cytometry: DPPIV/CD26 Antibody (11D7) [NBP2-02154] - Analysis of Hela cells, using anti-CD26 antibody, (Red) compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/DPPIV-CD26-Antibody-11D7-Flow-Cytometry-NBP2-02154-img0006.jpg)
![Western Blot: DPPIV/CD26 Antibody (11D7) [NBP2-02154] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD26 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD26.](http://images.novusbio.com/fullsize/DPPIV-CD26-Antibody-11D7-Western-Blot-NBP2-02154-img0008.jpg)
![Immunohistochemistry-Paraffin: DPPIV/CD26 Antibody (11D7) [NBP2-02154] - Staining of paraffin-embedded liver tissue using anti-CD26 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DPPIV-CD26-Antibody-11D7-Immunohistochemistry-Paraffin-NBP2-02154-img0007.jpg)
![Western Blot: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Western blot analysis of DPPIV/CD26 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse lung tissue lysate Lane 2: mouse heart tissue lysate Lane 3: human kidney tissue lysate Lane 4: Raji cell lysate Lane 5: PC-3 cell lysate](http://images.novusbio.com/fullsize/DPPIV-CD26-Antibody-JM11-42-Western-Blot-NBP2-78791-img0007.jpg "Western Blot: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Western blot analysis of DPPIV/CD26 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse lung tissue lysate Lane 2: mouse heart tissue lysate Lane 3: human kidney tissue lysate Lane 4: Raji cell lysate Lane 5: PC-3 cell lysate")
![Immunocytochemistry/Immunofluorescence: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Staining CD26 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/DPPIV-CD26-Antibody-JM11-42-Immunocytochemistry-Immunofluorescence-NBP2-78791-img0005.jpg)
![Immunohistochemistry-Paraffin: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Analysis of paraffin-embedded human liver tissue using anti-CD26 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/DPPIV-CD26-Antibody-JM11-42-Immunohistochemistry-Paraffin-NBP2-78791-img0006.jpg)
![Flow Cytometry: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Analysis of Hela cells with CD26 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/DPPIV-CD26-Antibody-JM11-42-Flow-Cytometry-NBP2-78791-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Staining CD26 in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/DPPIV-CD26-Antibody-JM11-42-Immunocytochemistry-Immunofluorescence-NBP2-78791-img0002.jpg)
![Immunohistochemistry-Paraffin: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Analysis of paraffin-embedded rat epididymis tissue using anti-CD26 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/DPPIV-CD26-Antibody-JM11-42-Immunohistochemistry-Paraffin-NBP2-78791-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - All lanes: Western blot analysis of CD26 with anti-CD26 antibody at 1:500 dilution.Lane 1: Wild-type Hela whole cell lysate (10 ug). Lane 2: CD26 knockout Hela whole cell lysate (10 ug).NBP2-78791 was shown to specifically react with CD26 in wild-type Hela cells. NO band was observed when CD26 knockout sample was tested. Wild-type and CD26 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/DPPIV-CD26-Antibody-JM11-42-Knockout-Validated-NBP2-78791-img0009.jpg)