| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 647 | DDX0141A647-100 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93792-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-16982-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52678-0.025mg | |||
![Western Blot: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Western blot analysis of CD40 Ligand/TNFSF5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse liver tissue lysate Lane 2: rat liver tissue lysate Lane 2: mouse bone marrow tissue lysate](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Western-Blot-NBP2-66756-img0007.jpg "Western Blot: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Western blot analysis of CD40 Ligand/TNFSF5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse liver tissue lysate Lane 2: rat liver tissue lysate Lane 2: mouse bone marrow tissue lysate")
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Staining CD40L in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunocytochemistry-Immunofluorescence-NBP2-66756-img0006.jpg)
![Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Analysis of paraffin-embedded human spleen tissue using anti- CD40L antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunohistochemistry-Paraffin-NBP2-66756-img0004.jpg)
![Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Analysis of THP-1 cells with CD40L antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Flow-Cytometry-NBP2-66756-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Staining CD40L in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunocytochemistry-Immunofluorescence-NBP2-66756-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Staining CD40L in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunocytochemistry-Immunofluorescence-NBP2-66756-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (LL48) [DDX0140P-100] - IF staining of hCD40L-transfected L cells with LL48 clone](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL48-Immunocytochemistry-Immunofluorescence-DDX0140P-100-img0005.jpg "Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (LL48) [DDX0140P-100] - IF staining of hCD40L-transfected L cells with LL48 clone")
![Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (LL48) [DDX0140P-100] - FACS staining of hCD40L-transfected L cells with LL48 clone](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL48-Flow-Cytometry-DDX0140P-100-img0002.jpg)
![Neutralization: CD40 Ligand/TNFSF5 Antibody (LL48) [DDX0140P-100] - Inhibition of IL12 production by CD40L-stimulated human monocyte-derived DCs by LL48 clone (Stax AM et al, 2008, J ImmunolMethods)](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL48-Neutralization-DDX0140P-100-img0003.jpg)
![Western Blot: CD40 Ligand/TNFSF5 Antibody [NBP1-91770] - Analysis in control (vector only transfected HEK293T lysate) and cD40LG over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-Western-Blot-NBP1-91770-img0007.jpg "Western Blot: CD40 Ligand/TNFSF5 Antibody [NBP1-91770] - Analysis in control (vector only transfected HEK293T lysate) and cD40LG over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells.")
![Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP1-91770] - Staining of human rectum shows strong cytoplasmic positivity in leukocytes.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-Immunohistochemistry-Paraffin-NBP1-91770-img0011.jpg)
![Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP1-91770] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-Immunohistochemistry-Paraffin-NBP1-91770-img0008.jpg)
![Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP1-91770] - Staining of human lymph node shows strong cytoplasmic positivity in leukocytes.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-Immunohistochemistry-Paraffin-NBP1-91770-img0009.jpg)
![Immunohistochemistry: CD40 Ligand/TNFSF5 Antibody [NBP1-91770] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-Immunohistochemistry-NBP1-91770-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - IF staining of hCD40L-transfected cellswith LL2 clone](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL2-Immunocytochemistry-Immunofluorescence-DDX0141P-100-img0005.jpg "Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - IF staining of hCD40L-transfected cellswith LL2 clone")
![Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - FACS staining of hCD40L-transfected Lcellswith LL2 clone](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL2-Flow-Cytometry-DDX0141P-100-img0002.jpg)
![Blocking/Neutralizing: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - LL2 inhibitsthe stimulatory effect of the CD40L-transfected L cells on synovial fibroblasts Rissoan et al, Clin Exp Immunol. 1996, 106(3):481-90.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL2-Blocking-Neutralizing-DDX0141P-100-img0003.jpg)
![Western Blot: Nuclear Factor Erythroid 2 Related Factor 1 Antibody [NBP2-93792] - Western blot analysis of extracts of various cell lines, using Nuclear Factor Erythroid 2 Related Factor 1 antibody (NBP2-93792) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Nuclear Factor Erythroid 2 Related Factor 1 Antibody-Western Blot-NBP2-93792-img0009.jpg "Western Blot: Nuclear Factor Erythroid 2 Related Factor 1 Antibody [NBP2-93792] - Western blot analysis of extracts of various cell lines, using Nuclear Factor Erythroid 2 Related Factor 1 antibody (NBP2-93792) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: Nuclear Factor Erythroid 2 Related Factor 1 Antibody [NBP2-93792] - Analysis of HeLa cells using Nuclear Factor Erythroid 2 Related Factor 1 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Nuclear-Factor-Erythroid-2-Related-Factor-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93792-img0001.jpg)
![Immunohistochemistry-Paraffin: Nuclear Factor Erythroid 2 Related Factor 1 Antibody [NBP2-93792] - Paraffin-embedded rat heart using Nuclear Factor Erythroid 2 Related Factor 1 .](http://images.novusbio.com/fullsize/Nuclear-Factor-Erythroid-2-Related-Factor-1-Antibody-Immunohistochemistry-Paraffin-NBP2-93792-img0008.jpg)
![Immunohistochemistry-Paraffin: Nuclear Factor Erythroid 2 Related Factor 1 Antibody [NBP2-93792] - Paraffin-embedded human placenta using Nuclear Factor Erythroid 2 Related Factor 1 .](http://images.novusbio.com/fullsize/Nuclear-Factor-Erythroid-2-Related-Factor-1-Antibody-Immunohistochemistry-Paraffin-NBP2-93792-img0005.jpg)
![Immunohistochemistry-Paraffin: Nuclear Factor Erythroid 2 Related Factor 1 Antibody [NBP2-93792] - Paraffin-embedded mouse brain using Nuclear Factor Erythroid 2 Related Factor 1 .](http://images.novusbio.com/fullsize/Nuclear-Factor-Erythroid-2-Related-Factor-1-Antibody-Immunohistochemistry-Paraffin-NBP2-93792-img0007.jpg)
![Western Blot: CD40 Ligand/TNFSF5 Antibody [NBP1-59186] - Sample Type: Human Fetal Muscle Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 1 ug/ml Peptide Concentration: 5 ug/ml Lysate Quantity: 25 ug/lane/lane Gel Concentration: 0.12](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-Western-Blot-NBP1-59186-img0006.jpg "Western Blot: CD40 Ligand/TNFSF5 Antibody [NBP1-59186] - Sample Type: Human Fetal Muscle Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 1 ug/ml Peptide Concentration: 5 ug/ml Lysate Quantity: 25 ug/lane/lane Gel Concentration: 0.12")
![Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP1-59186] - Human thymus tissue at an antibody concentration of 5ug/ml.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-Immunohistochemistry-Paraffin-NBP1-59186-img0005.jpg)
![Western Blot: CD40 Ligand/TNFSF5 Antibody [NBP1-59186] - Titration: 0.2-1 ug/ml, Positive Control: Human Muscle.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-Western-Blot-NBP1-59186-img0004.jpg)
![Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (MR-1) [NBP1-47234] - Surface staining of activated murine splenocytes (PHA + PMA + ionomycin for 6 hours) using anti-CD154 (MR-1) FITC.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-MR-1-Flow-Cytometry-NBP1-47234-img0001.jpg "Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (MR-1) [NBP1-47234] - Surface staining of activated murine splenocytes (PHA + PMA + ionomycin for 6 hours) using anti-CD154 (MR-1) FITC.")
![Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP3-16982] - Staining of human lymph node shows high expression.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-Immunohistochemistry-Paraffin-NBP3-16982-img0005.jpg "Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP3-16982] - Staining of human lymph node shows high expression.")
![Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP3-16982] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-Immunohistochemistry-Paraffin-NBP3-16982-img0001.jpg)
![Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP3-16982] - Analysis in human lymph node and cerebral cortex tissues using Anti-CD40LG antibody. Corresponding CD40LG RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-Immunohistochemistry-Paraffin-NBP3-16982-img0004.jpg)
![Western Blot: Nuclear Factor Erythroid 2 Related Factor 1 Antibody [NBP2-87955] - NFE2L1 (nuclear factor (erythroid-derived 2)-like 1) Antibody (against the middle region of NFE2L1) (50ug) validated by WB using Jurkat cell lysate at 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/Nuclear-Factor-Erythroid-2-Related-Factor-1-Antibody-Western-Blot-NBP2-87955-img0001.jpg "Western Blot: Nuclear Factor Erythroid 2 Related Factor 1 Antibody [NBP2-87955] - NFE2L1 (nuclear factor (erythroid-derived 2)-like 1) Antibody (against the middle region of NFE2L1) (50ug) validated by WB using Jurkat cell lysate at 0.2-1 ug/ml.")
![Western Blot: Nuclear Factor Erythroid 2 Related Factor 1 Antibody [NBP2-87955] - Host: Rabbit. Target Name: NFE2L1. Sample Tissue: Human HepG2 Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/Nuclear-Factor-Erythroid-2-Related-Factor-1-Antibody-Western-Blot-NBP2-87955-img0002.jpg)
![Western Blot: Nuclear Factor Erythroid 2 Related Factor 1 Antibody [NBP2-87953] - WB Suggested Anti-NFE2L1 Antibody. Titration: 1.0 ug/ml. Positive Control: THP-1 Whole Cell](http://images.novusbio.com/fullsize/Nuclear-Factor-Erythroid-2-Related-Factor-1-Antibody-Western-Blot-NBP2-87953-img0001.jpg "Western Blot: Nuclear Factor Erythroid 2 Related Factor 1 Antibody [NBP2-87953] - WB Suggested Anti-NFE2L1 Antibody. Titration: 1.0 ug/ml. Positive Control: THP-1 Whole Cell")
![Western Blot: Nuclear Factor Erythroid 2 Related Factor 1 Antibody [NBP2-87953] - Host: Rabbit. Target Name: NFE2L1. Sample Tissue: Human HepG2 Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/Nuclear-Factor-Erythroid-2-Related-Factor-1-Antibody-Western-Blot-NBP2-87953-img0002.jpg)
![Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (hu5c8 (Ruplizumab)) - Chimeric [NBP2-52678] - Flow-cytometry using the anti-CD40L research biosimilar antibody Ruplizumab. Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Ruplizimab (NBP2-52678, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-hu5c8-Ruplizumab-Chimeric-Flow-Cytometry-NBP2-52678-img0003.jpg "Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (hu5c8 (Ruplizumab)) - Chimeric [NBP2-52678] - Flow-cytometry using the anti-CD40L research biosimilar antibody Ruplizumab. Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Ruplizimab (NBP2-52678, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")