| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80775-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65336-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43338-0.5mg | |||
| BSA Free | NBP1-43338-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76304-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20094-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81085-0.025mg | |||
![Western Blot: IL6R Antibody (OTI2F4) - Azide and BSA Free [NBP2-71029] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL6R.](http://images.novusbio.com/fullsize/IL6R-Antibody-OTI2F4-Azide-and-BSA-Free-Western-Blot-NBP2-71029-img0001.jpg "Western Blot: IL6R Antibody (OTI2F4) - Azide and BSA Free [NBP2-71029] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL6R.")
![Immunocytochemistry/Immunofluorescence: IL6R Antibody (OTI2F4) - Azide and BSA Free [NBP2-71029] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY IL6R.](http://images.novusbio.com/fullsize/IL6R-Antibody-OTI2F4-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71029-img0003.jpg)
![Immunohistochemistry: IL6R Antibody (OTI2F4) - Azide and BSA Free [NBP2-71029] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/IL6R-Antibody-OTI2F4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71029-img0002.jpg)
![Western Blot: CD31/PECAM-1 Antibody (7-A1-R) [NBP3-32145] - Western blot analysis of CD31/PECAM-1 on different lysates with Mouse anti-CD31/PECAM-1 antibody (NBP3-32145) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: Jurkat cell lysate Lane 3: U-937 cell lysate Lane 4: HeLa cell lysate (negative) Lane 5: MDA-MB-231 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 83 kDa Observed band size: 130 kDa Exposure time: 1 minute 58 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32145) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32145_mouse-cd31-pecam-1-mab-7-a1-r-278202416331211.jpg "Western Blot: CD31/PECAM-1 Antibody (7-A1-R) [NBP3-32145] - Western blot analysis of CD31/PECAM-1 on different lysates with Mouse anti-CD31/PECAM-1 antibody (NBP3-32145) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: Jurkat cell lysate Lane 3: U-937 cell lysate Lane 4: HeLa cell lysate (negative) Lane 5: MDA-MB-231 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 83 kDa Observed band size: 130 kDa Exposure time: 1 minute 58 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32145) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD31/PECAM-1 Antibody (7-A1-R) [NBP3-32145] - Immunohistochemical analysis of paraffin-embedded human appendix tissue with Mouse anti-CD31/PECAM-1 antibody (NBP3-32145) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32145) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32145_mouse-cd31-pecam-1-mab-7-a1-r-298202414115443.jpg)
![Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (7-A1-R) [NBP3-32145] - Immunocytochemistry analysis of THP-1 (positive) and HeLa (negative) labeling CD31/PECAM-1 with Mouse anti-CD31/PECAM-1 antibody (NBP3-32145) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD31/PECAM-1 antibody (NBP3-32145) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32145_mouse-cd31-pecam-1-mab-7-a1-r-29202410484878.jpg)
![Flow Cytometry: CD1c/BDCA-1 Antibody (L161) [NBP2-62220] - Separation of human CD1c positive CD19 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood using anti-human CD1c (L161) purified antibody (concentration in sample 0,33 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-L161-Flow-Cytometry-NBP2-62220-img0004.jpg "Flow Cytometry: CD1c/BDCA-1 Antibody (L161) [NBP2-62220] - Separation of human CD1c positive CD19 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood using anti-human CD1c (L161) purified antibody (concentration in sample 0,33 ug/ml, GAM APC).")
![Flow Cytometry: CD1c/BDCA-1 Antibody (L161) [NBP2-62220] - Surface staining of human peripheral blood cells with anti-human CD1c (clone L161) PE.](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-L161-Flow-Cytometry-NBP2-62220-img0001.jpg)
![Flow (Cell Surface): CD1c/BDCA-1 Antibody (L161) [NBP2-62220] - Surface staining pattern of human peripheral whole blood stained using anti-human CD1c (L161) purified antibody (concentration in sample 0,33 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-L161-Flow-Cell-Surface-NBP2-62220-img0002.jpg)
![Flow Cytometry: CD1c/BDCA-1 Antibody (L161) [NBP2-62220] - Multicolor surface staining of human lymphocytes stained using anti-human CD1c (L161) purified antibody (concentration in sample 0,33 ug/ml, GAM APC) and anti-human CD19 (LT19) APC antibody (20 ul reagent / 100 ul of peripheral whole blood).](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-L161-Flow-Cytometry-NBP2-62220-img0003.jpg)
![Recombinant Human EGLN1/PHD2 GST (N-Term) Protein [H00054583-P01] - Analysis by 12.5% SDS-PAGE. Gel stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00054583-P01-1.jpg "Recombinant Human EGLN1/PHD2 GST (N-Term) Protein [H00054583-P01] - Analysis by 12.5% SDS-PAGE. Gel stained with Coomassie Blue.")
![Immunohistochemistry-Paraffin: Fucosyltransferase 2/FUT2 Antibody [NBP1-80775] - Staining of human duodenum shows cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Fucosyltransferase-2-FUT2-Antibody-Immunohistochemistry-Paraffin-NBP1-80775-img0003.jpg "Immunohistochemistry-Paraffin: Fucosyltransferase 2/FUT2 Antibody [NBP1-80775] - Staining of human duodenum shows cytoplasmic positivity in glandular cells.")
![Western Blot: MCPIP1/ZC3H12A Antibody (HL1442) - Azide and BSA Free [NBP3-25765] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZC3H12A antibody [HL1442] (NBP3-25765) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25765_rabbit-mcpip1-zc3h12a-mab-hl1442-azide-and-bsa-free-2120249413347.jpg "<b>Genetic Strategies Validation. </b>Western Blot: MCPIP1/ZC3H12A Antibody (HL1442) - Azide and BSA Free [NBP3-25765] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZC3H12A antibody [HL1442] (NBP3-25765) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Genetic Strategies Validation. </b>Western Blot: MCPIP1/ZC3H12A Antibody (HL1442) - Azide and BSA Free [NBP3-25765] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 | 20 | 20 | 10 | 10 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZC3H12A antibody [HL1442] (NBP3-25765) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25765_rabbit-mcpip1-zc3h12a-mab-hl1442-azide-and-bsa-free-2120249351328.jpg)
![Western Blot: MCPIP1/ZC3H12A Antibody (HL1442) - Azide and BSA Free [NBP3-25765] - Untreated (-) and treated (+) THP-1 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZC3H12A antibody [HL1442] (NBP3-25765) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25765_rabbit-mcpip1-zc3h12a-mab-hl1442-azide-and-bsa-free-2120249432887.jpg)
![Western Blot: MCPIP1/ZC3H12A Antibody (HL1442) - Azide and BSA Free [NBP3-25765] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ZC3H12A antibody [HL1442] (NBP3-25765) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25765_rabbit-mcpip1-zc3h12a-mab-hl1442-azide-and-bsa-free-212024939128.jpg)
![Flow Cytometry: CD31/PECAM1 Antibody (LCI-4) [NB100-65336] - Staining of porcine peripheral blood lymphocytes.](http://images.novusbio.com/fullsize/CD31-PECAM1-Antibody-LCI-4-Flow-Cytometry-NB100-65336-img0001.jpg "Flow Cytometry: CD31/PECAM1 Antibody (LCI-4) [NB100-65336] - Staining of porcine peripheral blood lymphocytes.")
![Immunohistochemistry-Paraffin: CXCR2/IL-8 RB Antibody (5E8-C7-F10) [NBP1-43338] - CXCR2positive breast cancer cells in the human brain. CXCR2-red fluorochrome, nucleus-DAPI. Antibody used at dilution of 1:100. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/CXCR2-IL-8-RB-Antibody-5E8-C7-F10-Immunohistochemistry-Paraffin-NBP1-43338-img0005.jpg "Immunohistochemistry-Paraffin: CXCR2/IL-8 RB Antibody (5E8-C7-F10) [NBP1-43338] - CXCR2positive breast cancer cells in the human brain. CXCR2-red fluorochrome, nucleus-DAPI. Antibody used at dilution of 1:100. This image was submitted via customer Review.")
![Flow Cytometry: CXCR2/IL-8 RB Antibody (5E8-C7-F10) [NBP1-43338] - Analysis using the PE conjugate of NBP1-43338. Staining of normal human peripheral blood cells with Anti-Human CD4 APC (left) or Anti-Human CD56 (NCAM) APC (right) and Anti-Human CD182 (CXCR2) PE. Cells in the lymphocyte gate were used for analysis.](http://images.novusbio.com/fullsize/CXCR2-IL-8-RB-Antibody-5E8-C7-F10-Flow-Cytometry-NBP1-43338-img0004.jpg)
![Flow Cytometry: CXCR2/IL-8 RB Antibody (5E8-C7-F10) [NBP1-43338] - Staining of normal human peripheral blood cells with 0.25 ug of Mouse IgG1 kappa Isotype Control Purified (left) or 0.25 ug of Anti-Human CD182 (CXCR2) Purified (right) followed by F(ab')2 Anti-Mouse IgG PE . Cells in the large scatter population were used for analysis.](http://images.novusbio.com/fullsize/CXCR2-IL-8-RB-Antibody-5E8-C7-F10-Flow-Cytometry-NBP1-43338-img0002.jpg)
![Western Blot: TCF-12/HTF4 Antibody [NBP1-76304] - HeLa cell lysate with TCF12 antibody at (A) 0.5 and (B) 1 ug/mL.](http://images.novusbio.com/fullsize/TCF-12-HTF4-Antibody-Western-Blot-NBP1-76304-img0001.jpg "Western Blot: TCF-12/HTF4 Antibody [NBP1-76304] - HeLa cell lysate with TCF12 antibody at (A) 0.5 and (B) 1 ug/mL.")
![Immunocytochemistry/ Immunofluorescence: TCF-12/HTF4 Antibody - BSA Free [NBP1-76304] - Immunocytochemistry of TCF-12/HTF4 in HeLa cells with TCF-12/HTF4 antibody at 10 u/mL.](http://images.novusbio.com/fullsize/nbp1-76304_rabbit-polyclonal-tcf-12-htf4-antibody-2711202419463462.jpg)
![Western Blot: LYVE-1 Antibody (JF0979) [NBP2-66940] - Western blot analysis of LYVE-1 on different lysates with Rabbit anti-LYVE1 antibody at 1/500 dilution.Lane 1: MCF-7 cell lysateLane 2: SW480 cell lysateLane 3: A431 cell lysateLane 4: HUVEC cell lysateLysates/proteins at 10 µg/Lane.Predicted band size: 35 kDaObserved band size: 35 kDaExposure time: 2 minutes;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-66940_rabbit-monoclonal-lyve-1-antibody-jf0979-western-blot-1572024145956..jpg "Western Blot: LYVE-1 Antibody (JF0979) [NBP2-66940] - Western blot analysis of LYVE-1 on different lysates with Rabbit anti-LYVE1 antibody at 1/500 dilution.Lane 1: MCF-7 cell lysateLane 2: SW480 cell lysateLane 3: A431 cell lysateLane 4: HUVEC cell lysateLysates/proteins at 10 µg/Lane.Predicted band size: 35 kDaObserved band size: 35 kDaExposure time: 2 minutes;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Western Blot: LYVE-1 Antibody (JF0979) [NBP2-66940] - Western blot analysis of LYVE-1 on different lysates with Rabbit anti-LYVE-1 antibody at 1/1,000 dilution.Lane 1: A549-si NT cell lysateLane 2: A549-si LYVE-1 cell lysateLysates/proteins at 10 µg/Lane.Predicted band size: 35 kDaObserved band size: 35 kDaExposure time: 3 minutes;4-20% SDS-PAGE gel.NBP2-66940 was shown to specifically react with LYVE-1 in A549-si NT cells. Weakened band was observed when A549-si LYVE-1 sample was tested. A549-si NT and Hela-si LYVE-1 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1702-29, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-66940_rabbit-monoclonal-lyve-1-antibody-jf0979-western-blot-15720241537..jpg)
![Flow Cytometry: LYVE-1 Antibody (JF0979) [NBP2-66940] - Analysis of HUVEC cells with LYVE1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/LYVE-1-Antibody-JF0979-Flow-Cytometry-NBP2-66940-img0001.jpg)
![Flow Cytometry: Calreticulin Antibody (SAIC-16D-6B9) - Azide and BSA Free [NBP3-20094] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-16D-6B9 (NBP3-20094, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20094_rabbit-calreticulin-mab-saic-16d-6b9-azide-and-bsa-free-2742023924252.png "Flow Cytometry: Calreticulin Antibody (SAIC-16D-6B9) - Azide and BSA Free [NBP3-20094] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-16D-6B9 (NBP3-20094, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Immunocytochemistry/Immunofluorescence: Calreticulin Antibody (SAIC-16D-6B9) - Azide and BSA Free [NBP3-20094] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-16D-6B9 (NBP3-20094) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20094, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20094_rabbit-calreticulin-mab-saic-16d-6b9-azide-and-bsa-free-2742023924253.png)
![Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (MJ7/18) - Chimeric [NBP2-81085] - Immunofluorescence staining of fixed mouse splenocytes with Endoglin/CD105 Antibody (MJ7/18) [NBP2-81085]. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of MJ7/18 [NBP2-81085] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining in a small subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81085, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MJ7-18-Chimeric-Immunocytochemistry-Immunofluorescence-NBP2-81085-img0001.jpg "Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (MJ7/18) - Chimeric [NBP2-81085] - Immunofluorescence staining of fixed mouse splenocytes with Endoglin/CD105 Antibody (MJ7/18) [NBP2-81085]. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of MJ7/18 [NBP2-81085] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining in a small subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81085, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.")