| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26877-25ul | |||
| Azide and BSA Free | NBP3-44280 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-91212-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49516-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62554-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB72272-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89960-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48364 | |||
![Western Blot: Endoglin/CD105 Antibody [NBP1-91212] - Analysis in human cell line U-138 MG.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-Western-Blot-NBP1-91212-img0006.jpg "Western Blot: Endoglin/CD105 Antibody [NBP1-91212] - Analysis in human cell line U-138 MG.")
![Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody [NBP2-49516] - Analysis in human placenta and pancreas tissues. Corresponding ENG RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-Immunohistochemistry-Paraffin-NBP2-49516-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody [NBP2-49516] - Analysis in human placenta and pancreas tissues. Corresponding ENG RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody [NBP2-49516] - Staining of human testis shows moderate membranous positivity in peritubular myoid cells.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-Immunohistochemistry-Paraffin-NBP2-49516-img0008.jpg)
![Immunohistochemistry: Endoglin/CD105 Antibody [NBP2-49516] - Staining of human placenta shows membranous positivity in trophoblasts.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-Immunohistochemistry-NBP2-49516-img0001.jpg)
![Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody [NBP2-49516] - Staining of human kidney shows strong membranous positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-Immunohistochemistry-Paraffin-NBP2-49516-img0006.jpg)
![Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody [NBP2-49516] - Staining of human pancreas shows low positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-Immunohistochemistry-Paraffin-NBP2-49516-img0007.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/1806R) - Azide and BSA Free [NBP2-54355] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/1806R)](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-1806R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54355-img0001.jpg "Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/1806R) - Azide and BSA Free [NBP2-54355] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/1806R)")
![Flow Cytometry: CD34 Antibody (HPCA1/1806R) - Azide and BSA Free [NBP2-54355] - Flow Cytometric Analysis of human Jurkat cells using CD34 Antibody (HPCA1/1806R) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-1806R-Azide-and-BSA-Free-Flow-Cytometry-NBP2-54355-img0002.jpg)
![Western Blot: IL6R Antibody (OTI2F4) - Azide and BSA Free [NBP2-71029] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL6R.](http://images.novusbio.com/fullsize/IL6R-Antibody-OTI2F4-Azide-and-BSA-Free-Western-Blot-NBP2-71029-img0001.jpg "Western Blot: IL6R Antibody (OTI2F4) - Azide and BSA Free [NBP2-71029] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL6R.")
![Immunocytochemistry/Immunofluorescence: IL6R Antibody (OTI2F4) - Azide and BSA Free [NBP2-71029] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY IL6R.](http://images.novusbio.com/fullsize/IL6R-Antibody-OTI2F4-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71029-img0003.jpg)
![Immunohistochemistry: IL6R Antibody (OTI2F4) - Azide and BSA Free [NBP2-71029] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/IL6R-Antibody-OTI2F4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71029-img0002.jpg)
![Western Blot: CD31/PECAM-1 Antibody (7-A1-R) [NBP3-32145] - Western blot analysis of CD31/PECAM-1 on different lysates with Mouse anti-CD31/PECAM-1 antibody (NBP3-32145) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: Jurkat cell lysate Lane 3: U-937 cell lysate Lane 4: HeLa cell lysate (negative) Lane 5: MDA-MB-231 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 83 kDa Observed band size: 130 kDa Exposure time: 1 minute 58 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32145) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32145_mouse-cd31-pecam-1-mab-7-a1-r-278202416331211.jpg "Western Blot: CD31/PECAM-1 Antibody (7-A1-R) [NBP3-32145] - Western blot analysis of CD31/PECAM-1 on different lysates with Mouse anti-CD31/PECAM-1 antibody (NBP3-32145) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: Jurkat cell lysate Lane 3: U-937 cell lysate Lane 4: HeLa cell lysate (negative) Lane 5: MDA-MB-231 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 83 kDa Observed band size: 130 kDa Exposure time: 1 minute 58 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32145) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD31/PECAM-1 Antibody (7-A1-R) [NBP3-32145] - Immunohistochemical analysis of paraffin-embedded human appendix tissue with Mouse anti-CD31/PECAM-1 antibody (NBP3-32145) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32145) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32145_mouse-cd31-pecam-1-mab-7-a1-r-298202414115443.jpg)
![Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (7-A1-R) [NBP3-32145] - Immunocytochemistry analysis of THP-1 (positive) and HeLa (negative) labeling CD31/PECAM-1 with Mouse anti-CD31/PECAM-1 antibody (NBP3-32145) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD31/PECAM-1 antibody (NBP3-32145) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32145_mouse-cd31-pecam-1-mab-7-a1-r-29202410484878.jpg)
![Western Blot: TRAILR3/TNFRSF10C Antibody (JE58-51) [NBP3-32257] - Western blot analysis of TRAILR3/TNFRSF10C on different lysates with Rabbit anti-TRAILR3/TNFRSF10C antibody (NBP3-32257) at 1/1,000 dilution. Lane 1: JAR cell lysate Lane 2: Jurkat cell lysate Lane 3: A549 cell lysate Lane 4: HeLa cell lysate Lane 5: Raji cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 27 kDa Observed band size: 27/70/80 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32257) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32257_rabbit-trailr3-tnfrsf10c-mab-je58-51-27820241529552.jpg "Western Blot: TRAILR3/TNFRSF10C Antibody (JE58-51) [NBP3-32257] - Western blot analysis of TRAILR3/TNFRSF10C on different lysates with Rabbit anti-TRAILR3/TNFRSF10C antibody (NBP3-32257) at 1/1,000 dilution. Lane 1: JAR cell lysate Lane 2: Jurkat cell lysate Lane 3: A549 cell lysate Lane 4: HeLa cell lysate Lane 5: Raji cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 27 kDa Observed band size: 27/70/80 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32257) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: TRAILR3/TNFRSF10C Antibody (JE58-51) [NBP3-32257] - Immunocytochemistry analysis of Jurkat cells labeling TRAILR3/TNFRSF10C with Rabbit anti-TRAILR3/TNFRSF10C antibody (NBP3-32257) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-TRAILR3/TNFRSF10C antibody (NBP3-32257) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32257_rabbit-trailr3-tnfrsf10c-mab-je58-51-2920241114817.jpg)
![Flow Cytometry: TRAILR3/TNFRSF10C Antibody (JE58-51) [NBP3-32257] - Flow cytometric analysis of Jurkat cells labeling TRAILR3/TNFRSF10C. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32257, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32257_rabbit-trailr3-tnfrsf10c-mab-je58-51-29202416372111.jpg)
![Western Blot: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of My10 (NBP2-62554) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa, though a second isoform at 35.2kDa is also present, with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. NBP2-62554 successfully detected both isoforms of human CD34.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Western-Blot-NBP2-62554-img0003.jpg "Western Blot: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of My10 (NBP2-62554) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa, though a second isoform at 35.2kDa is also present, with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. NBP2-62554 successfully detected both isoforms of human CD34.")
![Immunohistochemistry: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Immunohistochemical staining of human placenta tissue using anti-CD34 antibody. My10 Anti-CD34 staining of paraffin embedded human placenta tissue using the rabbit-chimeric version of My10 (NBP2-62554). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62554 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Strong endothelial staining of within villi may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Immunohistochemistry-NBP2-62554-img0002.jpg)
![Flow Cytometry: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Flow-cytometry using anti-CD34 antibody My10. Human leukocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of My10 (NBP2-62554, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Flow-Cytometry-NBP2-62554-img0001.jpg)
![Recombinant Human EGLN1/PHD2 GST (N-Term) Protein [H00054583-P01] - Analysis by 12.5% SDS-PAGE. Gel stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00054583-P01-1.jpg "Recombinant Human EGLN1/PHD2 GST (N-Term) Protein [H00054583-P01] - Analysis by 12.5% SDS-PAGE. Gel stained with Coomassie Blue.")
