| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80120-50ug | |||
.png "CD4 was detected in immersion fixed paraffin-embedded sections of mouse spleen using Rabbit Anti-Mouse CD4, Monoclonal Antibody (Catalog# NBP3-14741) at 1:250 dilution at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=")
DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." alt="CD4 was detected in immersion fixed paraffin-embedded sections of mouse spleen using Rabbit Anti-Mouse CD4, Monoclonal Antibody (Catalog# NBP3-14741) at 1:250 dilution at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08764 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87760-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43982 | |||
| BSA Free | NBP2-61415-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86095-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37871-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43907 | |||
| BSA Free | NBP2-52866-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75977SS | |||
| Azide and BSA Free | NBP2-80764 | |||
![Western Blot: Listeria monocytogenes p60 Antibody (p6007) - Azide and BSA Free [NBP2-80120] - Analysis using Listeria monocytogenes p60, mAb (P6007) (Prod. No. NBP2-80120) at 1:5000 dilution.](http://images.novusbio.com/fullsize/Listeria-monocytogenes-p60-Antibody-p6007-Azide-and-BSA-Free-Western-Blot-NBP2-80120-img0001.jpg "Western Blot: Listeria monocytogenes p60 Antibody (p6007) - Azide and BSA Free [NBP2-80120] - Analysis using Listeria monocytogenes p60, mAb (P6007) (Prod. No. NBP2-80120) at 1:5000 dilution.")
![ELISA: Rat IFN-beta ELISA Kit (Colorimetric) [NBP3-06753] - Samples were spiked with high concentrations of Rat IFN-beta and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-06753_rat-ifn-beta-elisa-kit-colorimetric-13220241613093.jpg "ELISA: Rat IFN-beta ELISA Kit (Colorimetric) [NBP3-06753] - Samples were spiked with high concentrations of Rat IFN-beta and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat IFN-beta ELISA Kit (Colorimetric) [NBP3-06753] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-06753_rat-ifn-beta-elisa-kit-colorimetric-4320248595118.jpg)
![Western Blot: CD4 Antibody (BLR167J) [NBP3-14741] - Whole cell lysate (10 ug) from NIH 3T3, BW5147.3, mouseThymus, 70Z/3, and EL4 cells prepared using NETN lysisbuffer. Antibody: Rabbit anti-CD4 recombinant monoclonalantibody [BLR167J] used at 1:1000.Secondary: HRP-conjugated goat anti-rabbit IgGChemiluminescence with an exposure timeof 30 seconds. Lower Panel: Rabbit anti-Actin recombinantmonoclonal antibody [BLR057F].](http://images.novusbio.com/fullsize/CD4-Antibody-BLR167J-Western-Blot-NBP3-14741-img0003.jpg)
![Immunohistochemistry-Paraffin: CD4 Antibody (BLR167J) [NBP3-14741] - Mouse lung, : Rabbit anti-CD4 recombinant monoclonal antibody [BLR167J]. Secondary: HRP-conjugated goat anti-rabbit IgG Substrate: DAB.](http://images.novusbio.com/fullsize/CD4-Antibody-BLR167J-Immunohistochemistry-Paraffin-NBP3-14741-img0006.jpg)
![Immunohistochemistry-Paraffin: CD4 Antibody (BLR167J) [NBP3-14741] - Mouse spleen, Rabbit anti-CD4 recombinant monoclonal antibody [BLR167J]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CD4-Antibody-BLR167J-Immunohistochemistry-Paraffin-NBP3-14741-img0004.jpg)
![Immunohistochemistry-Paraffin: CD4 Antibody (BLR167J) [NBP3-14741] - Mouse gut, Rabbit anti-CD4 recombinant monoclonal antibody [BLR167J]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CD4-Antibody-BLR167J-Immunohistochemistry-Paraffin-NBP3-14741-img0005.jpg)
![Immunoprecipitation: CD4 Antibody [NBP3-14741] - s: Whole cell lysate (200 ugper IP reaction; 10% of IP loaded) from mouse thymus.Antibodies: Rabbit anti-CD4 recombinant monoclonalantibody used for IP at 6 ul/200ug lysate. CD4 was also immunoprecipitated by anotherantibody against a different epitope of CD4 For blotting immunoprecipitated CD4, was used at 1:1000. Chemiluminescence withan exposure time of 30 seconds.](http://images.novusbio.com/fullsize/CD4-Antibody-Immunoprecipitation-NBP3-14741-img0001.jpg)
![Immunohistochemistry-Paraffin: Complement C3d Antibody (C3D/2891) [NBP3-07741] - Formalin-fixed, paraffin-embedded human hepatocellular carcinoma stained with Complement C3d Antibody (C3D/2891) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Complement-C3d-Antibody-C3D-2891-Immunohistochemistry-Paraffin-NBP3-07741-img0003.jpg "Immunohistochemistry-Paraffin: Complement C3d Antibody (C3D/2891) [NBP3-07741] - Formalin-fixed, paraffin-embedded human hepatocellular carcinoma stained with Complement C3d Antibody (C3D/2891) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.")
![Immunohistochemistry-Paraffin: Complement C3d Antibody (C3D/2891) [NBP3-07741] - Formalin-fixed, paraffin-embedded human rejected kidney transplant stained with Complement C3d Antibody (C3D/2891) at 2ug/ml in PBS. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Complement-C3d-Antibody-C3D-2891-Immunohistochemistry-Paraffin-NBP3-07741-img0002.jpg)
![Protein Array: Complement C3d Antibody (C3D/2891) [NBP3-07741] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Complement C3d Mouse Monoclonal Antibody (C3D/2891).](http://images.novusbio.com/fullsize/Complement-C3d-Antibody-C3D-2891-Protein-Array-NBP3-07741-img0001.jpg)
![Western Blot: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Western blot analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Western-Blot-NBP1-87760-img0021.jpg "<b>Genetic Strategies Validation. </b>Western Blot: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Western blot analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Staining in human appendix and skeletal muscle tissues using anti-NFKB2 antibody. Corresponding NFKB2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87760-img0020.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Staining of human cerebral cortex, kidney, lymph node and testis using Anti-NFKB2 antibody NBP1-87760 (A) shows similar protein distribution across tissues to independent antibody NBP1-87759 (B).](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87760-img0022.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Analysis using Anti-NFKB2 antibody NBP1-87760 (A) shows similar pattern to independent antibody NBP1-87759 (B).](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Western-Blot-NBP1-87760-img0023.jpg)
![Immunocytochemistry/Immunofluorescence: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87760-img0010.jpg)
![Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Staining of human testis.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87760-img0027.jpg)
![Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Staining of human appendix shows high expression.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87760-img0018.jpg)
![Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87760-img0019.jpg)
![Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Staining of human kidney.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87760-img0024.jpg)
![Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Staining of human lymph node.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87760-img0025.jpg)
![Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87760-img0026.jpg)
![Simple Western: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Simple Western lane view shows a specific band for NFKB2 in 0.2 mg/ml of RT4 lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Simple-Western-NBP1-87760-img0006.jpg)
![Simple Western: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Electropherogram image(s) of corresponding Simple Western lane view. NFkB p100/p52 antibody was used at 1:30 dilution on RT4 lysate(s).](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Simple-Western-NBP1-87760-img0007.jpg)
![ELISA: Human Complement C4a ELISA Kit (Colorimetric) [NBP2-70043] - Samples were spiked with high concentrations of Human Complement C4a and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-70043_human-complement-c4a-elisa-kit-colorimetric-132202416225233.jpg "ELISA: Human Complement C4a ELISA Kit (Colorimetric) [NBP2-70043] - Samples were spiked with high concentrations of Human Complement C4a and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Complement C4a ELISA Kit (Colorimetric) [NBP2-70043] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-70043_human-complement-c4a-elisa-kit-colorimetric-4320248595130.jpg)
![ELISA: Mouse Perforin ELISA Kit (Colorimetric) [NBP3-00452] - Samples were spiked with high concentrations of Mouse Perforin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-00452_mouse-perforin-elisa-kit-colorimetric-13220241685327.jpg "ELISA: Mouse Perforin ELISA Kit (Colorimetric) [NBP3-00452] - Samples were spiked with high concentrations of Mouse Perforin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Perforin ELISA Kit (Colorimetric) [NBP3-00452] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-00452_mouse-perforin-elisa-kit-colorimetric-432024903582.jpg)
![Western Blot: CTGF/CCN2 Antibody (CL5339) [NBP2-61415] - Analysis in human cell line U-251 MG.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-CL5339-Western-Blot-NBP2-61415-img0007.jpg "Western Blot: CTGF/CCN2 Antibody (CL5339) [NBP2-61415] - Analysis in human cell line U-251 MG.")
![Immunohistochemistry-Paraffin: pIgR Antibody [NBP1-86095] - Analysis in human duodenum and skeletal muscle tissues. Corresponding PIGR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/pIgR-Antibody-Immunohistochemistry-Paraffin-NBP1-86095-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: pIgR Antibody [NBP1-86095] - Analysis in human duodenum and skeletal muscle tissues. Corresponding PIGR RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: pIgR Antibody [NBP1-86095] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/pIgR-Antibody-Immunohistochemistry-Paraffin-NBP1-86095-img0014.jpg)
![Immunohistochemistry-Paraffin: pIgR Antibody [NBP1-86095] - Staining of human duodenum shows strong cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/pIgR-Antibody-Immunohistochemistry-Paraffin-NBP1-86095-img0012.jpg)
![Immunohistochemistry-Paraffin: pIgR Antibody [NBP1-86095] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/pIgR-Antibody-Immunohistochemistry-Paraffin-NBP1-86095-img0013.jpg)
![Immunohistochemistry-Paraffin: pIgR Antibody [NBP1-86095] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/pIgR-Antibody-Immunohistochemistry-Paraffin-NBP1-86095-img0015.jpg)
![Western Blot: MCPIP1/ZC3H12A Antibody [NBP2-37871] - Analysis in control (vector only transfected HEK293T lysate) and ZC3H12A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/MCPIP1-ZC3H12A-Antibody-Western-Blot-NBP2-37871-img0004.jpg "Western Blot: MCPIP1/ZC3H12A Antibody [NBP2-37871] - Analysis in control (vector only transfected HEK293T lysate) and ZC3H12A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: MCPIP1/ZC3H12A Antibody [NBP2-37871] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/MCPIP1-ZC3H12A-Antibody-Immunohistochemistry-Paraffin-NBP2-37871-img0008.jpg)
![Immunohistochemistry-Paraffin: MCPIP1/ZC3H12A Antibody [NBP2-37871] - Staining of human tonsil shows strong membranous positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/MCPIP1-ZC3H12A-Antibody-Immunohistochemistry-Paraffin-NBP2-37871-img0005.jpg)
![Immunohistochemistry-Paraffin: MCPIP1/ZC3H12A Antibody [NBP2-37871] - Staining of human colon shows moderate cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/MCPIP1-ZC3H12A-Antibody-Immunohistochemistry-Paraffin-NBP2-37871-img0006.jpg)
![Immunohistochemistry-Paraffin: MCPIP1/ZC3H12A Antibody [NBP2-37871] - Staining of human testis shows moderate cytoplasmic and nuclear positivity in Leydig cells.](http://images.novusbio.com/fullsize/MCPIP1-ZC3H12A-Antibody-Immunohistochemistry-Paraffin-NBP2-37871-img0007.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining in human endometrium and liver tissues. Corresponding ZEB1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining in human endometrium and liver tissues. Corresponding ZEB1 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: ZEB1 Antibody (CL0151) [NBP2-52866] - Analysis of extracts from RH-30 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-ZEB1 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensi](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Western-Blot-NBP2-52866-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Analysis in human endometrium and liver tissues using AMAb90510 antibody. Corresponding ZEB1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0013.jpg)
![Western Blot: ZEB1 Antibody (CL0151) [NBP2-52866] - Lane 1: Marker [kDa] Lane 2: Human cell line RH-30 Lane 3: Human cell line CACO-2](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Western-Blot-NBP2-52866-img0007.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0009.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining of human endometrium shows strong nuclear positivity in stromal cells.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0010.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining of human smooth muscle shows strong nuclear positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0011.jpg)
![Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Western-Blot-NBP2-75977-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (NBP2-75977) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at an approximatel molecular weight of 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control.This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Knockdown-Validated-NBP2-75977-img0003.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Immunocytochemistry-Immunofluorescence-NBP2-75977-img0001.jpg)
![Simple Western: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20ug/ml. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Simple-Western-NBP2-75977-img0004.jpg)
![Simple Western: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Simple Western lane view shows lysates of MCF +/- DMOG, BioSpherix MSCs in hypoxic conditions, and BioSpherix MSCs in normoxic conditions loaded at 0.5 mg/ml. A band was detected for HIF-1 alpha at approximately 116 kDa (as indicated) using NBP2-75977 (1:100 dilution) followed by Anti-Rabbit Secondary Antibody (042-206, ProteinSimple). This experiment was conducted under standard assay conditions, and using the 12-230 kDa separation module (SW-W004). Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Simple-Western-NBP2-75977-img0005.jpg)
.png){kind=link}