| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64852-0.025mg | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | DDX0207A488 | |||
| Alexa Fluor 546 | DDX0207A546 | |||
| Alexa Fluor 647 | DDX0207A647 | |||
| Biotin | DDX0207B | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25200SS | |||
| Azide and BSA Free | NBP2-80635 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | DDX0470A488-100 | |||
| Alexa Fluor 546 | DDX0470A546-100 | |||
| Alexa Fluor 647 | DDX0470A647-100 | |||
| Biotin | DDX0470B | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27405 | |||
| BSA Free | NBP2-27404SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76680-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76465-0.025mg | |||
![Western Blot: SH2D1A Antibody (1C9) [H00004068-M01] - Analysis of SH2D1A expression in transfected 293T cell line by SH2D1A monoclonal antibody (M01), clone 1C9.Lane 1: SH2D1A transfected lysate(14.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SH2D1A-Antibody-1C9-Western-Blot-H00004068-M01-img0005.jpg "Western Blot: SH2D1A Antibody (1C9) [H00004068-M01] - Analysis of SH2D1A expression in transfected 293T cell line by SH2D1A monoclonal antibody (M01), clone 1C9.Lane 1: SH2D1A transfected lysate(14.2 KDa).Lane 2: Non-transfected lysate.")
![Flow Cytometry: SH2D1A Antibody (1C9) [H00004068-M01] - Intracellular staining of human T cells with SH2D1A monoclonal antibody or isotype control antibody, followed by a Polyclonal Goat Anti-Mouse/RPE secondary antibody. Grey filled histogram: Isotype control, empty blue histogram: SH2D1A antibody. Flow cytometry image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SH2D1A-Antibody-1C9-Flow-Cytometry-H00004068-M01-img0007.jpg)
![Western Blot: SH2D1A Antibody (1C9) [H00004068-M01] - SH2D1A monoclonal antibody (M01), clone 1C9 Analysis of SH2D1A expression in Jurkat.](http://images.novusbio.com/fullsize/SH2D1A-Antibody-1C9-Western-Blot-H00004068-M01-img0004.jpg)
![ELISA: SH2D1A Antibody (1C9) [H00004068-M01] - Detection limit for recombinant GST tagged SH2D1A is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SH2D1A-Antibody-1C9-ELISA-H00004068-M01-img0006.jpg)
![Immunohistochemistry-Frozen: CD1a Antibody (NA1/34-HLK) [NB100-64852] - Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD1a antibody followed by HISTAR detection system. Medium power](http://images.novusbio.com/fullsize/CD1a-Antibody-NA1-34-HLK-Immunohistochemistry-Frozen-NB100-64852-img0007.jpg "Immunohistochemistry-Frozen: CD1a Antibody (NA1/34-HLK) [NB100-64852] - Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD1a antibody followed by HISTAR detection system. Medium power")
![Flow Cytometry: CD1a Antibody (NA1/34-HLK) [NB100-64852] - Analysis using the FITC conjugate of NB100-64852. Staining of MOLT 4 cells.](http://images.novusbio.com/fullsize/CD1a-Antibody-NA1-34-HLK-Flow-Cytometry-NB100-64852-img0005.jpg)
![Immunohistochemistry-Frozen: CD1a Antibody (NA1/34-HLK) [NB100-64852] - mmunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD1a antibody followed by HISTAR detection system. Low power](http://images.novusbio.com/fullsize/CD1a-Antibody-NA1-34-HLK-Immunohistochemistry-Frozen-NB100-64852-img0006.jpg)
![Flow Cytometry: CD1a Antibody (NA1/34-HLK) [NB100-64852] - Analysis using the Biotin conjugate of NB100-64852. Staining of MOLT 4 cells with CD1a antibody.](http://images.novusbio.com/fullsize/CD1a-Antibody-NA1-34-HLK-Flow-Cytometry-NB100-64852-img0004.jpg)
![Western Blot: CCR7 Antibody (SR36-04) [NBP2-67324] - Analysis of CCR7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysateLane 2: MCF-7 cell lysate Lane 3: PC-12 cell lysate Lane 4: Daudi cell lysate](http://images.novusbio.com/fullsize/CCR7-Antibody-SR36-04-Western-Blot-NBP2-67324-img0009.jpg "Western Blot: CCR7 Antibody (SR36-04) [NBP2-67324] - Analysis of CCR7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysateLane 2: MCF-7 cell lysate Lane 3: PC-12 cell lysate Lane 4: Daudi cell lysate")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CCR7 Antibody (SR36-04) [NBP2-67324] - Gross and histological appearance of lung excised from 13 weeks of age PyMT transgenic females. CRE treated PyMT transgenic females showed decreased frequency of mammary tumor-derived lung metastases. Immunohistochemistry staining against Ccr7 in lung tissues was done and representative images of tissue sections from each group were taken at 200X and 40X magnification. Scale bars = 50um. Histogram data was presented as mean +/- SEM. * p < 0.05 vs. control. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6643/11/2/410), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCR7-Antibody-SR36-04-Immunocytochemistry-Immunofluorescence-NBP2-67324-img0006.jpg)
![Immunohistochemistry-Paraffin: CCR7 Antibody (SR36-04) [NBP2-67324] - Analysis of paraffin-embedded human tonsil tissue using anti-CCR7 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CCR7-Antibody-SR36-04-Immunohistochemistry-Paraffin-NBP2-67324-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: CCR7 Antibody (SR36-04) [NBP2-67324] - Gross and histological appearance of lung excised from 13 weeks of age PyMT transgenic females. CRE treated PyMT transgenic females showed decreased frequency of mammary tumor-derived lung metastases. Level of Ccr7 protein in lung tissues was analyzed by Western blot. Gapdh was used as an internal control. * p < 0.05 vs. control. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6643/11/2/410), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCR7-Antibody-SR36-04-Western-Blot-NBP2-67324-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CCR7 Antibody (SR36-04) [NBP2-67324] - Staining CCR7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CCR7-Antibody-SR36-04-Immunocytochemistry-Immunofluorescence-NBP2-67324-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CCR7 Antibody (SR36-04) [NBP2-67324] - Staining CCR7 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CCR7-Antibody-SR36-04-Immunocytochemistry-Immunofluorescence-NBP2-67324-img0002.jpg)
![Immunohistochemistry-Paraffin: CCR7 Antibody (SR36-04) [NBP2-67324] - Analysis of paraffin-embedded human spleen tissue using anti-CCR7 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CCR7-Antibody-SR36-04-Immunohistochemistry-Paraffin-NBP2-67324-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CCR7 Antibody (SR36-04) [NBP2-67324] - Downregulation of CCR7 gene by siRNA inhibits gene expression of MMP9, c-FOS, c-JUN and CCL21 in vitro. Western blot of proteins extracted from MCF7 cell against CCR7 and GAPDH. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6643/11/2/410), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCR7-Antibody-SR36-04-Knockdown-Validated-NBP2-67324-img0007.jpg)
![Western Blot: CCR7 Antibody (SR36-04) [NBP2-67324] - Curcumae radix extract treatment exhibited anti-metastatic effects on primary tumor of MMTV-PyMT transgenic mouse. (A) Quantitative analysis of real-time PCR was done using genes associated with breast cancer metastasis. The expression levels of Ccr7, c-Fos, Mmp9, Ccl21, & Ccl19 were determined by qRT-PCR. The Hprt gene was used as input control, respectively, * p < 0.05 & ** p < 0.005 vs. control. (B) Western blot of proteins extracted from tumor tissues in the PyMT transgenic model against Ccr7 & Gapdh. Histogram data was presented as mean ± SEM. * p < 0.05 vs. control. (C) Western blot of proteins extracted from tumor tissues against Phosphorylated Akt, Phosphorylated Ampk, Her2, ERalpha, Gapdh & beta -actin. (D) Quantitative analysis of real time PCR was done by using genes associated with apoptosis. The expression levels of Jun, Akt, & Tnf were determined by qRT-PCR. Data are representative of three independent experiments & are expressed as the mean ± SD. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30781353), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-67324_rabbit-monoclonal-ccr7-antibody-sr36-04-3102024161224.jpg)
![Immunocytochemistry/Immunofluorescence: DC-SIGN/CD209 Antibody (109H12.03) [DDX0207] - Murine peyer patch: DC-SIGN](http://images.novusbio.com/fullsize/DC-SIGN-CD209-Antibody-109H12-03-Immunocytochemistry-Immunofluorescence-DDX0207-img0007.jpg "Immunocytochemistry/Immunofluorescence: DC-SIGN/CD209 Antibody (109H12.03) [DDX0207] - Murine peyer patch: DC-SIGN")
![Immunohistochemistry-Paraffin: DC-SIGN/CD209 Antibody (109H12.03) [DDX0207] - Tonsil](http://images.novusbio.com/fullsize/DC-SIGN-CD209-Antibody-109H12-03-Immunohistochemistry-Paraffin-DDX0207-img0008.jpg)
![Flow Cytometry: DC-SIGN/CD209 Antibody (109H12.03) [DDX0207] - Analysis of Alexa Fluor (R) 647 conjugate of DDX0207. FACS staining of CD14+ CD209+ cells. (CD14+ cells cultured 5 days with GM CSF and IL-10)](http://images.novusbio.com/fullsize/DC-SIGN-CD209-Antibody-109H12-03-Flow-Cytometry-DDX0207-img0011.jpg)
![Flow Cytometry: DC-SIGN/CD209 Antibody (109H12.03) [DDX0207] - Analysis using the Alexa Fluor (R) 488 conjugate of DDX0207. A) Staining of DC-SIGN transfected HeLa cells. B) IF staining of DC-SIGN transfected cells with 109H12.03.](http://images.novusbio.com/fullsize/DC-SIGN-CD209-Antibody-109H12-03-Flow-Cytometry-DDX0207-img0009.jpg)
![Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - CD3+ T-Cells isolated from PBMCs stained with anti-CD2 Alexa Fluor 488 (BL1). Image from a verified customer review.](http://images.novusbio.com/fullsize/CD2-Antibody-RPA-2-10-Flow-Cytometry-NBP2-25200-img0013.jpg "Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - CD3+ T-Cells isolated from PBMCs stained with anti-CD2 Alexa Fluor 488 (BL1). Image from a verified customer review.")
![Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Cell surface analysis of CD2 in 10^6 human lymphocytes using 0.25 ug of CD2 RPA-2.10 antibody; Shaded histogram represents cells without antibody; green represents isotype control ; red represents CD2 antibody. Goat anti-mouse IgG PE conjugated secondary was used in this test.](http://images.novusbio.com/fullsize/CD2-Antibody-RPA-2-10-Flow-Cytometry-NBP2-25200-img0001.jpg)
![Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-25200. Staining of CD2 in 10^6 human lymphocytes using 10 ul (0.25 ug) of this antibody. Shaded histogram represents cells without antibody; green represents isotype control ; red represents anti-CD2.](http://images.novusbio.com/fullsize/CD2-Antibody-RPA-2-10-Flow-Cytometry-NBP2-25200-img0008.jpg)
![Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Analysis using the Allophycocyanin conjugate of NBP2-25200. Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control APC (open histogram) or Anti-Human CD2 APC (filled histogram). Cells in the lymphocyte gate were used for analysis.](http://images.novusbio.com/fullsize/CD2-Antibody-RPA-2-10-Flow-Cytometry-NBP2-25200-img0009.jpg)
![Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Analysis using the Biotin conjugate of NBP2-25200. Staining of normal human peripheral blood cells with 0.125 ug of Mouse IgG1 kappa Isotype Control Biotin (open histogram) or 0.125 ug of Anti-Human CD2 Biotin (filled histogram) followed by Streptavidin PE.](http://images.novusbio.com/fullsize/CD2-Antibody-RPA-2-10-Flow-Cytometry-NBP2-25200-img0010.jpg)
![Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Analysis using the FITC conjugate of NBP2-25200. Staining of Human peripheral blood lymphocytes with RPA-2.10 FITC.](http://images.novusbio.com/fullsize/CD2-Antibody-RPA-2-10-Flow-Cytometry-NBP2-25200-img0011.jpg)
![Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Analysis using the PE/Cy5 conjugate of NBP2-25200. Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE-Cy5 (open histogram) or Anti-Human CD2 PE-Cy5 (filled histogram). Total cells were used for analysis.](http://images.novusbio.com/fullsize/CD2-Antibody-RPA-2-10-Flow-Cytometry-NBP2-25200-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: CD40/TNFRSF5 Antibody (G28-5) [DDX0130P-100] - CD40-transfected L cells stained.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-G28-5-Immunocytochemistry-Immunofluorescence-DDX0130P-100-img0002.jpg "Immunocytochemistry/Immunofluorescence: CD40/TNFRSF5 Antibody (G28-5) [DDX0130P-100] - CD40-transfected L cells stained.")
![Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (G28-5) [DDX0130P-100] - Human cervical tissue. a: normal cervical epithelium. b: cervical intraepithelial neoplasia. c: squamous cell carcinoma .](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-G28-5-Immunohistochemistry-Paraffin-DDX0130P-100-img0001.jpg)
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining pattern of human peripheral blood stained using anti-human CD28 (CD28.2) purified antibody (concentration in sample 1,6 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0009.jpg "Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining pattern of human peripheral blood stained using anti-human CD28 (CD28.2) purified antibody (concentration in sample 1,6 ug/ml) GAM APC.")
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) purified.](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0001.jpg)
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) PE.](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0002.jpg)
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) PerCP-CyTM5.5](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0003.jpg)
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) APC.](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0004.jpg)
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) PerCP.](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0005.jpg)
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) FITC.](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0006.jpg)
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) PE-CyTM7.](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0007.jpg)
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Separation of human CD28 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD28 (CD28.2) purified antibody (concentration in sample 1,6 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: HLA G Antibody (MEM-G/9) [NB500-314] - Staining of HLA-G1 transfectants (LCL-HLA-G1) using Alexa Fluor 488 conjugated anti-human HLA-G (MEM-G/9).](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-9-Immunocytochemistry-Immunofluorescence-NB500-314-img0006.jpg "Immunocytochemistry/Immunofluorescence: HLA G Antibody (MEM-G/9) [NB500-314] - Staining of HLA-G1 transfectants (LCL-HLA-G1) using Alexa Fluor 488 conjugated anti-human HLA-G (MEM-G/9).")
![Flow Cytometry: HLA G Antibody (MEM-G/9) [NB500-314] - Surface staining of HLA-G1 transfectants (LCL-HLA-G1) using anti-HLA-G (MEM-G/9) PE.](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-9-Flow-Cytometry-NB500-314-img0012.jpg)
![Flow Cytometry: HLA G Antibody (MEM-G/9) [NB500-314] - Analysis of APC conjugate of NB500-314. Double surface staining of HLA-G1 transfectants (viable cells gate) using anti-human HLA-G (MEM-G/9) APC and anti-human HLA-G (MEM-G/11) FITC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-9-Flow-Cytometry-NB500-314-img0010.jpg)
![Flow Cytometry: HLA G Antibody (MEM-G/9) [NB500-314] - Surface staining of HLA-G1 transfectants (LCL-HLA-G1) using anti-HLA-G (MEM-G/9) FITC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-9-Flow-Cytometry-NB500-314-img0011.jpg)
![Immunoprecipitation: HLA G Antibody (MEM-G/9) [NB500-314] - Immunoprecipitation of HLA-G from HLA-G1 transfectanted lysates (LCL-HLA-G1) by anti-human HLA-G (MEM-G/9, NB500-314) and protein G. HLA-G was detected by anti-human HLA-G (4H84) and goat anti-mouse HRP in cell lysate (Lanes 1) and in the immunoprecipitate (Lanes 2).](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-9-Immunoprecipitation-NB500-314-img0009.jpg)
![Western Blot: TLR3 Antibody (619F7.06) [DDX0470P-100] - Western-blot analysisof human (h) and canine(c) PBMC and monocytes with 619F7.06](http://images.novusbio.com/fullsize/TLR3-Antibody-619F7-06-Western-Blot-DDX0470P-100-img0007.jpg "Western Blot: TLR3 Antibody (619F7.06) [DDX0470P-100] - Western-blot analysisof human (h) and canine(c) PBMC and monocytes with 619F7.06")
![Immunohistochemistry-Frozen: TLR3 Antibody (619F7.06) [DDX0470P-100] - Analysis using the Alexa Fluor (R) 488 conjugate of DDX0470P-100. Staining of human tonsil section.](http://images.novusbio.com/fullsize/TLR3-Antibody-619F7-06-Immunohistochemistry-Frozen-DDX0470P-100-img0003.jpg)
![Flow Cytometry: TLR3 Antibody (619F7.06) [DDX0470P-100] - Intracellular FACS staining of canine blood cells with 619F7.06](http://images.novusbio.com/fullsize/TLR3-Antibody-619F7-06-Flow-Cytometry-DDX0470P-100-img0006.jpg)
![Immunohistochemistry-Paraffin: TLR3 Antibody (619F7.06) [DDX0470P-100] - Paraffin staining of Canine keratinocytes with TLR3 Antibody](http://images.novusbio.com/fullsize/TLR3-Antibody-619F7-06-Immunohistochemistry-Paraffin-DDX0470P-100-img0001.jpg)
![Flow Cytometry: TLR3 Antibody (619F7.06) [DDX0470P-100] - Intracellular FACS staining of human breast carcinoma CAMA-1 cellline with 619F7.06](http://images.novusbio.com/fullsize/TLR3-Antibody-619F7-06-Flow-Cytometry-DDX0470P-100-img0004.jpg)
![Flow Cytometry: TLR3 Antibody (619F7.06) [DDX0470P-100] - Intracellular FACS staining of human monocyte-derived DCs and human EBV B cells with 619F7.06](http://images.novusbio.com/fullsize/TLR3-Antibody-619F7-06-Flow-Cytometry-DDX0470P-100-img0005.jpg)
![Western Blot: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis of TLR3 using TLR3 monoclonal antibody. Human intestine (A), human spleen (B), and mouse RAW lysate (C) probed with TLR3 antibody at 2 ug/ml. I goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Western-Blot-NBP2-27404-img0001.jpg "Western Blot: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis of TLR3 using TLR3 monoclonal antibody. Human intestine (A), human spleen (B), and mouse RAW lysate (C) probed with TLR3 antibody at 2 ug/ml. I goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Flow Cytometry: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis of TLR3 using TLR3 monoclonal antibody. Mouse splenocytes were probed with TLR3 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Flow-Cytometry-NBP2-27404-img0009.jpg)
![Flow Cytometry: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis using Azide Free version of NBP2-27404. TLR3 using TLR3 monoclonal antibody. Mouse RAW cells and splenocytes were probed with TLR3 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Flow-Cytometry-NBP2-27404-img0006.jpg)
![Flow Cytometry: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis using Azide Free version of NBP2-27404. TLR3 using TLR3 monoclonal antibody. Mouse RAW cells were probed with TLR3 antibody (red) and isotype control antibody (green) at 0.1 ug/10^6 cells.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Flow-Cytometry-NBP2-27404-img0007.jpg)
![Flow Cytometry: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis of TLR3 using TLR3 monoclonal antibody. Mouse RAW cells were probed with TLR3 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Flow-Cytometry-NBP2-27404-img0008.jpg)
![Flow Cytometry: TLR3 Antibody (27N3D4) - BSA Free [NBP2-27404] - An intracellular stain was performed on THP-1 cells with TLR3 Antibody [27N3D4] NBP2-27405AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/nbp2-27404_rat-monoclonal-tlr3-antibody-27n3d4-177202495942.jpg)
![Flow Cytometry: TLR3 Antibody (27N3D4) - BSA Free [NBP2-27404] - An intracellular stain was performed on Raw264.7 cells with TLR3 [27N3D4] Antibody NBP2-27405AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/nbp2-27404_rat-monoclonal-tlr3-antibody-27n3d4-17720241044614.jpg)
![Western Blot: TLR9 Antibody [NBP1-76680] - (G) Levels of TLR9 (NBP1-76680), COX4 (NB110-39115), MyD88, and NF-kB protein by Western blot. (H) Relative expression of TLR9 and COX4 protein. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fimmu.2018.01477/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR9-Antibody-Western-Blot-NBP1-76680-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: TLR9 Antibody [NBP1-76680] - (G) Levels of TLR9 (NBP1-76680), COX4 (NB110-39115), MyD88, and NF-kB protein by Western blot. (H) Relative expression of TLR9 and COX4 protein. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fimmu.2018.01477/full), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: TLR9 Antibody - BSA Free [NBP1-76680] - Immunofluorescence of TLR9 in Mouse Spleen tissue with TLR9 antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76680_rabbit-polyclonal-tlr9-antibody-271120242013147.jpg)
![Western Blot: TLR9 Antibody [NBP1-76680] - Analysis of TLR9 in Jurkat cell lysate with TLR9 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide](http://images.novusbio.com/fullsize/TLR9-Antibody-Western-Blot-NBP1-76680-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: TLR9 Antibody [NBP1-76680] - (A) Levels of COX4 (NB110-39115), TLR9 (NBP1-76680), MyD88, and NF-kB mRNA. (B) Levels of COX4 (NB110-39115), TLR9 (NBP1-76680), MyD88, and NF-kB protein by Western blot. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fimmu.2018.01477/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR9-Antibody-Western-Blot-NBP1-76680-img0007.jpg)
![Western Blot: TLR9 Antibody - BSA Free [NBP1-76680] - Levels of toll-like receptor (TLR) 9, myeloid differentiation factor 88 (MyD88), & nuclear factor (NF)-kappa B in lung tissues from animals with spontaneous breathing (CON), mechanical ventilation at normal tidal volume (NTV), high tidal volume (HTV), & ODN2088 pretreatment upon HTV stimulation (HTV + ODN). aP < 0.05 vs. CON group;bP < 0.05 vs. NTV group; & cP < 0.05 vs. HTV group. Image collected & cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fimmu.2018.01477/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-76680_rabbit-polyclonal-tlr9-antibody-31020241535698.jpg)
![Immunocytochemistry/ Immunofluorescence: TLR9 Antibody - BSA Free [NBP1-76680] - Immunocytochemistry of TLR9 in Jurkat cells with TLR9 antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76680_rabbit-polyclonal-tlr9-antibody-2711202420243383.jpg)
![Western Blot: MyD88 Antibody [NBP1-76465] - KO Validation in HeLa Cells. Loading: 10 ug of HeLa WT cell lysate or MyD88 KO cell lysate. Antibodies: MyD88 (2 ug/mL) and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76465_rabbit-polyclonal-myd88-antibody-2711202419463463.gif "<b>Genetic Strategies Validation. </b>Western Blot: MyD88 Antibody [NBP1-76465] - KO Validation in HeLa Cells. Loading: 10 ug of HeLa WT cell lysate or MyD88 KO cell lysate. Antibodies: MyD88 (2 ug/mL) and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Genetic Strategies Validation. </b>Western Blot: MyD88 Antibody [NBP1-76465] - Western Blot Validation of MyD88 in human cell lines. Loading: 15 ug of lysates per lane. Antibodies: NBP1-76465 (2 ug/mL) 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Predicted band size: 35 kDa](http://images.novusbio.com/fullsize/MyD88-Antibody-Western-Blot-NBP1-76465-img0010.jpg)
![Western Blot: MyD88 Antibody [NBP1-76465] - Animal Species ReactivityLoading: Lysates/proteins at 15 ug per lane.Antibodies: NBP1-76925 (2 ug/mL) or NBP1-76465 (2 μg/mL). 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/MyD88-Antibody-Western-Blot-NBP1-76465-img0007.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: MyD88 Antibody [NBP1-76465] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: MyD88 NBP1-76925 (2 ug/mL), MyD88 NBP1-76465 (2 ug/mL), beta-actin (1 ug/mL), and GAPDH (0.02 ug/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/MyD88-Antibody-Western-Blot-NBP1-76465-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: MyD88 Antibody [NBP1-76465] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human TissuesLoading: 15 ug of lysates per lane.Antibodies: MyD88 NBP1-76925 (2 ug/mL), MyD88 NBP1-76465 (2 ug/mL), beta-actin (1 ug/mL), and GAPDH (0.02 ug/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/MyD88-Antibody-Western-Blot-NBP1-76465-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: MyD88 Antibody - BSA Free [NBP1-76465] - Validation of MyD88 In HeLa Cells. Immunofluorescent analysis of methanol-fixed HeLa cells labeling MyD88 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.](http://images.novusbio.com/fullsize/nbp1-76465_rabbit-polyclonal-myd88-antibody-2711202420392736.jpg)
![Immunocytochemistry/ Immunofluorescence: Rabbit Polyclonal MyD88 Antibody [NBP1-76465] - Immunofluorescence Validation of MyD88 in Jurkat Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Jurkat cells labeling MyD88 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).](http://images.novusbio.com/fullsize/antibody/nbp1-76465_rabbit-polyclonal-myd88-antibody-imgenex-img-5499-immunocytochemistry-immunofluorescence-22102025124527.gif)
![Immunohistochemistry: Rabbit Polyclonal MyD88 Antibody [NBP1-76465] - Immunohistochemistry Validation of MyD88 in Human Colon. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-MYD88 antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/antibody/nbp1-76465_rabbit-polyclonal-myd88-antibody-imgenex-img-5499-immunohistochemistry-22102025124757.jpg)
![Immunohistochemistry: Rabbit Polyclonal MyD88 Antibody [NBP1-76465] - Immunohistochemistry Validation of MyD88 in Mouse Heart. Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-MYD88 antibody at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/antibody/nbp1-76465_rabbit-polyclonal-myd88-antibody-imgenex-img-5499-immunohistochemistry-22102025125425.jpg)