| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33660-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37329-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43996 | |||
| BSA Free | NBP2-52927-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90128-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13159SS | |||
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-33660] - Analysis in human esophagus and placenta tissues. Corresponding Chymase/CMA1/Mast Cell Chymase RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-Immunohistochemistry-Paraffin-NBP2-33660-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-33660] - Analysis in human esophagus and placenta tissues. Corresponding Chymase/CMA1/Mast Cell Chymase RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-33660] - Aged control whole mount choroid immunolabeled for (A) UEA lectin (blue), (B) tryptase (red), and (C) chymase (green). The vast majority of human choroidal MCs are of the MCT phenotype (tryptase+/chymase-). Only a small percentage of MCs are MCtc (tryptase+/chymase+) (arrows). Scale bar: 30 um. Image collected and cropped by CiteAb from the following publication (//iovs.arvojournals.org/article.aspx?doi=10.1167/iovs.17-22989), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33660-img0013.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-33660] - Staining of human placenta shows no positivity in trophoblastic cells as expected.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-Immunohistochemistry-Paraffin-NBP2-33660-img0012.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-33660] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-Immunohistochemistry-Paraffin-NBP2-33660-img0008.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-33660] - Staining of human colon shows strong cytoplasmic positivity in mast cell.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-Immunohistochemistry-Paraffin-NBP2-33660-img0009.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-33660] - Staining of human esophagus shows strong cytoplasmic positivity in mast cell.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-Immunohistochemistry-Paraffin-NBP2-33660-img0010.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-33660] - Staining of human lymph node shows strong cytoplasmic positivity in mast cell.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-Immunohistochemistry-Paraffin-NBP2-33660-img0011.jpg)
![Immunocytochemistry/ Immunofluorescence: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-33660] - Aged control whole mount choroid immunolabeled for (A) UEA lectin (blue), (B) tryptase (red), & (C) chymase (green). The vast majority of human choroidal MCs are of the MCT phenotype (tryptase+/chymase−). Only a small percentage of MCs are MCtc (tryptase+/chymase+) (arrows). Scale bar: 30 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29164232), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-33660_rabbit-polyclonal-chymase-cma1-mast-cell-chymase-antibody-310202415291960.jpg)
![Western Blot: ATR Antibody [NB100-323] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATR antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ATR-Antibody-Western-Blot-NB100-323-img0004.jpg "Western Blot: ATR Antibody [NB100-323] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATR antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: ATR Antibody [NB100-323] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ATR antibody NB100-323 lot 3 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). ATR was also immunoprecipitated by a previous lot (lot 2) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/ATR-Antibody-Western-Blot-NB100-323-img0002.jpg)
![Western Blot: ATR Antibody [NB100-323] - H1299 (human lung cancer cells) and HCC 44 (human liver cancer cells). Antibody at 1:500. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ATR-Antibody-Western-Blot-NB100-323-img0003.jpg)
![Western Blot: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249422625.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - ZEB1 antibody [HL2245] detects ZEB1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZEB1 stained by ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249374983.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - ZEB1 antibody [HL2245] detects ZEB1 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. ZEB1 stained by ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249374923.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249422673.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249393552.jpg)
![Western Blot: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-212024941465.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - ZEB1 antibody [HL2245] detects ZEB1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. ZEB1 stained by ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249415991.jpg)
![Western Blot: PBEF/Visfatin/NAMPT Antibody (BLR058F) [NBP2-76368] - Detection of human and mouse NAMPT/PBEF/Visfatin by WB of RKO, MCF-7, K-562, GaMG, HEK293T, HeLa, U2OS, LNCaP, Hep-G2, Jurkat, and NIH 3T3 lysate. Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal [BLR058F] (NBP2-76368). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Lower Panel: Rabbit anti-GAPDH.](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-BLR058F-Western-Blot-NBP2-76368-img0004.jpg "Western Blot: PBEF/Visfatin/NAMPT Antibody (BLR058F) [NBP2-76368] - Detection of human and mouse NAMPT/PBEF/Visfatin by WB of RKO, MCF-7, K-562, GaMG, HEK293T, HeLa, U2OS, LNCaP, Hep-G2, Jurkat, and NIH 3T3 lysate. Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal [BLR058F] (NBP2-76368). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Lower Panel: Rabbit anti-GAPDH.")
![Immunocytochemistry: PBEF/Visfatin/NAMPT Antibody (BLR058F) [NBP2-76368] - Detection of human NAMPT/PBEF/Visfatin in FFPE NCI-H460 by ICC. Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal [BLR058F] (NBP2-76368). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-BLR058F-Immunocytochemistry-NBP2-76368-img0003.jpg)
![Immunohistochemistry: PBEF/Visfatin/NAMPT Antibody (BLR058F) [NBP2-76368] - Detection of human NAMPT/PBEF/Visfatin in FFPE ovarian carcinoma by IHC. Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal [BLR058F] (NBP2-76368). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-BLR058F-Immunohistochemistry-NBP2-76368-img0002.jpg)
![Flow Cytometry: PBEF/Visfatin/NAMPT Antibody (BLR058F) [NBP2-76368] - Rabbit antiNAMPT/PBEF/Visfatin recombinant monoclonal [BLR058F] or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-BLR058F-Flow-Cytometry-NBP2-76368-img0006.jpg)
![Immunohistochemistry: PBEF/Visfatin/NAMPT Antibody (BLR058F) [NBP2-76368] - Detection of human NAMPT/PBEF/Visfatin in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal [BLR058F] (NBP2-76368). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-BLR058F-Immunohistochemistry-NBP2-76368-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: PBEF/Visfatin/NAMPT Antibody (BLR058F) [NBP2-76368] - Detection of human NAMPT/PBEF/Visfatin by WB of immunoprecipitates from HEK293T lysate. Antibodies: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal [BLR058F] (NBP2-76368), rabbit anti-NAMPT/PBEF/Visfatin antibodies NB100-594, and one other rabbit anti-NAMPT/PBEF/Visfatin antibody.](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-BLR058F-Immunoprecipitation-NBP2-76368-img0005.jpg)
![Western Blot: SMURF2 Antibody [NBP2-81916] - Analysis of SMURF2 in C2C12 cell lysate with SMURF2 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/SMURF2-Antibody-Western-Blot-NBP2-81916-img0001.jpg "Western Blot: SMURF2 Antibody [NBP2-81916] - Analysis of SMURF2 in C2C12 cell lysate with SMURF2 antibody at 1 ug/ml.")
![Immunohistochemistry: SMURF2 Antibody - BSA Free [NBP2-81916] - Immunohistochemistry of SMURF2 in mouse liver tissue with SMURF2 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/nbp2-81916_rabbit-polyclonal-smurf2-antibody-2711202420285849.gif)
![Immunohistochemistry: SMURF2 Antibody - BSA Free [NBP2-81916] - Immunohistochemistry of SMURF2 in mouse liver tissue with SMURF2 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-81916_rabbit-polyclonal-smurf2-antibody-2711202419381720.jpg)
![Immunocytochemistry/ Immunofluorescence: SMURF2 Antibody - BSA Free [NBP2-81916] - Immunofluorescence of SMURF2 in human uterus tissue with SMURF2 antibody at 20 ug/ml.Green: SMURF2 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp2-81916_rabbit-polyclonal-smurf2-antibody-271120241958449.gif)
![Immunocytochemistry/ Immunofluorescence: SMURF2 Antibody - BSA Free [NBP2-81916] - Immunofluorescence of SMURF2 in mouse liver tissue with SMURF2 antibody at 20 ug/ml.Green: SMURF2 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp2-81916_rabbit-polyclonal-smurf2-antibody-2711202420432386.gif)
![Immunocytochemistry/ Immunofluorescence: SMURF2 Antibody - BSA Free [NBP2-81916] - Immunofluorescence of SMURF2 in mouse liver tissue with SMURF2 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-81916_rabbit-polyclonal-smurf2-antibody-2711202419475814.jpg)
![Immunohistochemistry: SMURF2 Antibody - BSA Free [NBP2-81916] - Immunohistochemistry of SMURF2 in human uterus tissue with SMURF2 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/nbp2-81916_rabbit-polyclonal-smurf2-antibody-2711202419514042.gif)
![Western Blot: ZEB1 Antibody (2A8H3) [NBP2-37329] - Western blot analysis using ZEB1 mAb against HEK293 (1) and ZEB1 (AA: 967-1108)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Western-Blot-NBP2-37329-img0003.jpg "Western Blot: ZEB1 Antibody (2A8H3) [NBP2-37329] - Western blot analysis using ZEB1 mAb against HEK293 (1) and ZEB1 (AA: 967-1108)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (2A8H3) [NBP2-37329] - Immunofluorescence analysis of Hela cells using ZEB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Immunocytochemistry-Immunofluorescence-NBP2-37329-img0004.jpg)
![Immunohistochemistry: ZEB1 Antibody (2A8H3) [NBP2-37329] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ZEB1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Immunohistochemistry-NBP2-37329-img0007.jpg)
![Flow Cytometry: ZEB1 Antibody (2A8H3) [NBP2-37329] - Flow cytometric analysis of Hela cells using ZEB1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Flow-Cytometry-NBP2-37329-img0005.jpg)
![Western Blot: ZEB1 Antibody (2A8H3) [NBP2-37329] - Western blot analysis using ZEB1 mAb against human ZEB1 recombinant protein. (Expected MW is 41.7 kDa)](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Western-Blot-NBP2-37329-img0002.jpg)
![Immunohistochemistry: ZEB1 Antibody (2A8H3) [NBP2-37329] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ZEB1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Immunohistochemistry-NBP2-37329-img0006.jpg)
![ELISA: ZEB1 Antibody (2A8H3) [NBP2-37329] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-ELISA-NBP2-37329-img0001.jpg)
![Western Blot: Rabbit Monoclonal FAT10 Antibody (JJ084-09) [NBP2-67917] - Western blot analysis of Ubiquitin D on different lysates with Rabbit anti-Ubiquitin D antibody (NBP2-67917) at 1/2,000 dilution.Lane 1: HeLa cell lysateLane 2: HepG2 cell lysateLane 3: NIH/3T3 cell lysateLysates/proteins at 20 µg/Lane.Predicted band size: 18 kDaObserved band size: 20 kDaExposure time: 26 seconds; ECL: K1801;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP2-67917) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67917_rabbit-monoclonal-fat10-antibody-jj084-09-western-blot-1312026165644.jpg "Western Blot: Rabbit Monoclonal FAT10 Antibody (JJ084-09) [NBP2-67917] - Western blot analysis of Ubiquitin D on different lysates with Rabbit anti-Ubiquitin D antibody (NBP2-67917) at 1/2,000 dilution.Lane 1: HeLa cell lysateLane 2: HepG2 cell lysateLane 3: NIH/3T3 cell lysateLysates/proteins at 20 µg/Lane.Predicted band size: 18 kDaObserved band size: 20 kDaExposure time: 26 seconds; ECL: K1801;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP2-67917) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: FAT10 Antibody (JJ084-09) [NBP2-67917] - ICC staining of Ubiquitin D in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP2-67917, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/FAT10-Antibody-JJ084-09-Immunocytochemistry-Immunofluorescence-NBP2-67917-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: FAT10 Antibody (JJ084-09) [NBP2-67917] - ICC staining of Ubiquitin D in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP2-67917, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/FAT10-Antibody-JJ084-09-Immunocytochemistry-Immunofluorescence-NBP2-67917-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: FAT10 Antibody (JJ084-09) [NBP2-67917] - ICC staining of Ubiquitin D in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP2-67917, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/FAT10-Antibody-JJ084-09-Immunocytochemistry-Immunofluorescence-NBP2-67917-img0002.jpg)
![Immunohistochemistry: Rabbit Monoclonal FAT10 Antibody (JJ084-09) [NBP2-67917] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Ubiquitin D antibody (NBP2-67917) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP2-67917) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-67917_rabbit-monoclonal-fat10-antibody-jj084-09-immunohistochemistry-paraffin-131202617029.jpg)
![Immunohistochemistry: Rabbit Monoclonal FAT10 Antibody (JJ084-09) [NBP2-67917] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Ubiquitin D antibody (NBP2-67917) at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP2-67917) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-67917_rabbit-monoclonal-fat10-antibody-jj084-09-immunohistochemistry-paraffin-131202617226.jpg)
![Immunohistochemistry: Rabbit Monoclonal FAT10 Antibody (JJ084-09) [NBP2-67917] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Ubiquitin D antibody (NBP2-67917) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP2-67917) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-67917_rabbit-monoclonal-fat10-antibody-jj084-09-immunohistochemistry-paraffin-131202617524.jpg)
![Immunohistochemistry-Paraffin: MMR/CD206/Mannose Receptor Antibody (CL0387) [NBP2-52927] -Analysis in human lung and skeletal muscle tissues using NBP2-52927 antibody. Corresponding MRC1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/antibody/nbp2-52927_mouse-monoclonal-mmr-cd206-mannose-receptor-antibody-cl0387-immunohistochemistry-paraffin-1662023104225..jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MMR/CD206/Mannose Receptor Antibody (CL0387) [NBP2-52927] -Analysis in human lung and skeletal muscle tissues using NBP2-52927 antibody. Corresponding MRC1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: MMR/CD206/Mannose Receptor Antibody (CL0387) [NBP2-52927] - Lane 1: Marker [kDa], Lane 2: Human liver tissue lysate](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-CL0387-Western-Blot-NBP2-52927-img0005.jpg)
![Immunohistochemistry-Paraffin: MMR/CD206/Mannose Receptor Antibody (CL0387) [NBP2-52927] -Staining of human lung shows strong membranous positivity in macrophages.](http://images.novusbio.com/fullsize/antibody/nbp2-52927_mouse-monoclonal-mmr-cd206-mannose-receptor-antibody-cl0387-immunohistochemistry-paraffin-1662023104335..jpg)
![Immunohistochemistry-Paraffin: MMR/CD206/Mannose Receptor Antibody (CL0387) [NBP2-52927] -Staining of human liver shows moderate positivity in kupffer cells.](http://images.novusbio.com/fullsize/antibody/nbp2-52927_mouse-monoclonal-mmr-cd206-mannose-receptor-antibody-cl0387-immunohistochemistry-paraffin-1662023104449..jpg)
![Immunohistochemistry-Paraffin: MMR/CD206/Mannose Receptor Antibody (CL0387) [NBP2-52927] -Staining of human rectum shows moderate positivity in lymphoid cells.](http://images.novusbio.com/fullsize/antibody/nbp2-52927_mouse-monoclonal-mmr-cd206-mannose-receptor-antibody-cl0387-immunohistochemistry-paraffin-1662023104552..jpg)
![Immunohistochemistry-Paraffin: MMR/CD206/Mannose Receptor Antibody (CL0387) [NBP2-52927] -Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-52927_mouse-monoclonal-mmr-cd206-mannose-receptor-antibody-cl0387-immunohistochemistry-paraffin-166202310476..jpg)
![Western Blot: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Lane 1: Wild-type HepG2 whole cell lysate (10 ug).Lane 2: CD206 knockdown HepG2 whole cell lysate (10 ug).Lane 3: CD206 knockdown HepG2 whole cell lysate (10 ug).NBP2-66956 was shown to specifically react with CD206 in wild-type Hela cells. Weakened bands were observed when CD206 knockdown samples were tested. Wild-type and CD206 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-JF0953-Western-Blot-NBP2-66956-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Lane 1: Wild-type HepG2 whole cell lysate (10 ug).Lane 2: CD206 knockdown HepG2 whole cell lysate (10 ug).Lane 3: CD206 knockdown HepG2 whole cell lysate (10 ug).NBP2-66956 was shown to specifically react with CD206 in wild-type Hela cells. Weakened bands were observed when CD206 knockdown samples were tested. Wild-type and CD206 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Staining Mannose Receptor in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-JF0953-Immunocytochemistry-Immunofluorescence-NBP2-66956-img0003.jpg)
![Western Blot: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Western blot analysis of MMR/CD206/Mannose Receptor on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse lung tissue lysate Lane 2: D3 cell lysate](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-JF0953-Western-Blot-NBP2-66956-img0005.jpg)
![Western Blot: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Western blot analysis of Mannose Receptor(CD206) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: Human lung tissue lysate Lane 2: HepG2 cell lysate](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-JF0953-Western-Blot-NBP2-66956-img0006.jpg)
![Western Blot: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Western blot analysis of Mannose Receptor(CD206) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: 293T cell lysateLane 2: MCF-7 cell lysate](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-JF0953-Western-Blot-NBP2-66956-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Staining Mannose Receptor in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-JF0953-Immunocytochemistry-Immunofluorescence-NBP2-66956-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Staining Mannose Receptor in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-JF0953-Immunocytochemistry-Immunofluorescence-NBP2-66956-img0002.jpg)
![Western Blot: RPL7 Antibody [NB100-2268] - Staufen1 co-fractionates with Gag and vRNA in gradient density fractionation analyses. HeLa cells were either mock transfected with empty vector, pcDNA3, or with a plasmid that expresses Staufen1-HA. The transfected cells were collected 24?h later, lysed and were either mock-treated or treated with RNAse A. The lysates were then fractionated on 5-50% sucrose gradients and 20 fractions were collected for further analysis by western blotting for viral and host proteins, as indicated. HIV-1 viral genomic RNA (vRNA, 9?kb) was assessed in each fraction by slot blot analysis. TL represents the total lysates. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/Journal/10.3389/fmicb.2012.00367/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RPL7-Antibody-Western-Blot-NB100-2268-img0002.jpg "Western Blot: RPL7 Antibody [NB100-2268] - Staufen1 co-fractionates with Gag and vRNA in gradient density fractionation analyses. HeLa cells were either mock transfected with empty vector, pcDNA3, or with a plasmid that expresses Staufen1-HA. The transfected cells were collected 24?h later, lysed and were either mock-treated or treated with RNAse A. The lysates were then fractionated on 5-50% sucrose gradients and 20 fractions were collected for further analysis by western blotting for viral and host proteins, as indicated. HIV-1 viral genomic RNA (vRNA, 9?kb) was assessed in each fraction by slot blot analysis. TL represents the total lysates. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/Journal/10.3389/fmicb.2012.00367/full), licensed under a CC-BY license.")
![Western Blot: RPL7 Antibody [NB100-2268] - Detection of Human and Mouse RPL7 on HeLa whole cell lysate using NB100-2268. RPL7 was immunoprecipitated by rabbit anti-RPL7 antibody NB100-2269.](http://images.novusbio.com/fullsize/RPL7-Antibody-Western-Blot-NB100-2268-img0001.jpg)
![Western Blot: RPL7 Antibody [NB100-2268] - Staufen1 co-fractionates with Gag and vRNA in gradient density fractionation analyses. HeLa cells were co-transfected with pNL 4-3 and Staufen1-HA. The presence of Staufen1-HA, precursor Gag and p24 were assessed in each fraction by western blotting analysis. HIV-1 viral genomic RNA (9?kb, vRNA) was assessed in each fraction by slot blot analysis. Staufen1, Gag and vRNA were quantitated in each fraction by densitometry and relative levels are depicted for each fraction (Blue: Staufen1-HA, Red: Gag, Green-vRNA). Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/Journal/10.3389/fmicb.2012.00367/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RPL7-Antibody-Western-Blot-NB100-2268-img0003.jpg)
![Western Blot: ZEB1 Antibody [NBP2-13159] - Analysis of Zeb1 in (1) HeLa (2) Cos7 (3) HepG2 and (4) Ntera2 cell lysates.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Western-Blot-NBP2-13159-img0002.jpg "Western Blot: ZEB1 Antibody [NBP2-13159] - Analysis of Zeb1 in (1) HeLa (2) Cos7 (3) HepG2 and (4) Ntera2 cell lysates.")
![Immunocytochemistry/Immunofluorescence: ZEB1 Antibody [NBP2-13159] - ZEB1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/ZEB1-Antibody-Immunocytochemistry-NBP2-13159-img0001.jpg)
![Simple Western: ZEB1 Antibody [NBP2-13159] - Lane view shows a specific band for ZEB1 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Simple-Western-NBP2-13159-img0003.jpg)