| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73912 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93792-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76298-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81332-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82582-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76799SS | |||
| Azide and BSA Free | NBP2-80926 | |||
![Western Blot: RIPK3/RIP3 Antibody (OTI1B3) [NBP2-45592] - Analysis of RIPK3/RIP3 using RIPK3/RIP3 antibody (OTI1B3) [NBP2-45592] in HEK293T cells transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIPK3. Observed molecular weight ~55 kDa. Theoretical molecular weight 56.7 kDa.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-1B3-Western-Blot-NBP2-45592-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: RIPK3/RIP3 Antibody (OTI1B3) [NBP2-45592] - Analysis of RIPK3/RIP3 using RIPK3/RIP3 antibody (OTI1B3) [NBP2-45592] in HEK293T cells transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIPK3. Observed molecular weight ~55 kDa. Theoretical molecular weight 56.7 kDa.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: RIPK3/RIP3 Antibody (OTI1B3) [NBP2-45592] - Analysis of Human Kidney tissue using RIPK3/RIP3 Antibody [NBP2-45592]. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-1B3-Immunohistochemistry-NBP2-45592-img0001.jpg)
![Immunohistochemistry: RIPK3/RIP3 Antibody (OTI1B3) [NBP2-45592] - Analysis of Carcinoma of Human thyroid tissue using RIPK3/RIP3 Antibody [NBP2-45592]. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-1B3-Immunohistochemistry-NBP2-45592-img0003.jpg)
![Western Blot: RIPK3/RIP3 Antibody (OTI1B3) [NBP2-45592] - Analysis of extracts (10ug) from 2 different cell lines using RIPK3/RIP3 antibody (OTI1B3) [NBP2-45592]. Observed molecular weight ~55 kDa. Theoretical molecular weight 56.7 kDa.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-1B3-Western-Blot-NBP2-45592-img0005.jpg)
![Immunohistochemistry: RIPK3/RIP3 Antibody (OTI1B3) [NBP2-45592] - Analysis of Carcinoma of Human pancreas tissue using RIPK3/RIP3 Antibody [NBP2-45592]. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-1B3-Immunohistochemistry-NBP2-45592-img0002.jpg)
![Western Blot: Nuclear Factor Erythroid 2 Related Factor 1 Antibody [NBP2-93792] - Western blot analysis of extracts of various cell lines, using Nuclear Factor Erythroid 2 Related Factor 1 antibody (NBP2-93792) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Nuclear Factor Erythroid 2 Related Factor 1 Antibody-Western Blot-NBP2-93792-img0009.jpg "Western Blot: Nuclear Factor Erythroid 2 Related Factor 1 Antibody [NBP2-93792] - Western blot analysis of extracts of various cell lines, using Nuclear Factor Erythroid 2 Related Factor 1 antibody (NBP2-93792) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: Nuclear Factor Erythroid 2 Related Factor 1 Antibody [NBP2-93792] - Analysis of HeLa cells using Nuclear Factor Erythroid 2 Related Factor 1 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Nuclear-Factor-Erythroid-2-Related-Factor-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93792-img0001.jpg)
![Immunohistochemistry-Paraffin: Nuclear Factor Erythroid 2 Related Factor 1 Antibody [NBP2-93792] - Paraffin-embedded rat heart using Nuclear Factor Erythroid 2 Related Factor 1 .](http://images.novusbio.com/fullsize/Nuclear-Factor-Erythroid-2-Related-Factor-1-Antibody-Immunohistochemistry-Paraffin-NBP2-93792-img0008.jpg)
![Immunohistochemistry-Paraffin: Nuclear Factor Erythroid 2 Related Factor 1 Antibody [NBP2-93792] - Paraffin-embedded human placenta using Nuclear Factor Erythroid 2 Related Factor 1 .](http://images.novusbio.com/fullsize/Nuclear-Factor-Erythroid-2-Related-Factor-1-Antibody-Immunohistochemistry-Paraffin-NBP2-93792-img0005.jpg)
![Immunohistochemistry-Paraffin: Nuclear Factor Erythroid 2 Related Factor 1 Antibody [NBP2-93792] - Paraffin-embedded mouse brain using Nuclear Factor Erythroid 2 Related Factor 1 .](http://images.novusbio.com/fullsize/Nuclear-Factor-Erythroid-2-Related-Factor-1-Antibody-Immunohistochemistry-Paraffin-NBP2-93792-img0007.jpg)
![Western Blot: FXR1 Antibody (JE40-57) [NBP3-32354] - Western blot analysis of FXR1 on different lysates with Rabbit anti-FXR1 antibody (NBP3-32354) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: A549 cell lysate Lane 3: K-562 cell lysate Lane 4: Jurkat cell lysate Lane 5: HepG2 cell lysate Lane 6: HeLa cell lysate Lane 7: C2C12 cell lysate Lane 8: NIH/3T3 cell lysate Lane 9: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 70 kDa Observed band size: 70/75 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32354) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32354_rabbit-fxr1-mab-je40-57-278202416331261.jpg "Western Blot: FXR1 Antibody (JE40-57) [NBP3-32354] - Western blot analysis of FXR1 on different lysates with Rabbit anti-FXR1 antibody (NBP3-32354) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: A549 cell lysate Lane 3: K-562 cell lysate Lane 4: Jurkat cell lysate Lane 5: HepG2 cell lysate Lane 6: HeLa cell lysate Lane 7: C2C12 cell lysate Lane 8: NIH/3T3 cell lysate Lane 9: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 70 kDa Observed band size: 70/75 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32354) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: FXR1 Antibody (JE40-57) [NBP3-32354] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-FXR1 antibody (NBP3-32354) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32354) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32354_rabbit-fxr1-mab-je40-57-29820241492613.jpg)
![Immunocytochemistry/ Immunofluorescence: FXR1 Antibody (JE40-57) [NBP3-32354] - Immunocytochemistry analysis of HeLa cells labeling FXR1 with Rabbit anti-FXR1 antibody (NBP3-32354) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FXR1 antibody (NBP3-32354) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32354_rabbit-fxr1-mab-je40-57-29202410334066.jpg)
![Flow Cytometry: FXR1 Antibody (JE40-57) [NBP3-32354] - Flow cytometric analysis of HeLa cells labeling FXR1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32354, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32354_rabbit-fxr1-mab-je40-57-392024884674.jpg)
![Immunohistochemistry-Paraffin: RPL3 Antibody [NBP2-20214] - 4T1 xenograft, using RPL3 antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/RPL3-Antibody-Immunohistochemistry-Paraffin-NBP2-20214-img0002.jpg "Immunohistochemistry-Paraffin: RPL3 Antibody [NBP2-20214] - 4T1 xenograft, using RPL3 antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![RPL3 antibody [N3C3] detects RPL3 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. RPL3 antibody [N3C3] (NBP2-20214) diluted at 1:500. br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-20214_rabbit-polyclonal-rpl3-antibody-immunohistochemistry-261120251823304.jpg)
![RPL3 antibody [N3C3] detects RPL3 protein at cytoplasm in mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. RPL3 antibody [N3C3] (NBP2-20214) diluted at 1:500. br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-20214_rabbit-polyclonal-rpl3-antibody-immunohistochemistry-261120251837249.jpg)
![Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RPL3 antibody [N3C3] (NBP2-20214) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-20214_rabbit-polyclonal-rpl3-antibody-western-blot-261120251825322.jpg)
![Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody [NBP1-31098] - Human lung adenocarcinoma. karyopherin alpha 2 antibody [C3], C-term diluted at 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunohistochemistry-Paraffin-NBP1-31098-img0008.jpg "Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody [NBP1-31098] - Human lung adenocarcinoma. karyopherin alpha 2 antibody [C3], C-term diluted at 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Importin alpha 2/KPNA2 Antibody [NBP1-31098] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with KPNA2 antibody [C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-NBP1-31098-img0011.jpg)
![karyopherin alpha 2 antibody [C3], C-term detects karyopherin alpha 2 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: karyopherin alpha 2 protein stained by karyopherin alpha 2 antibody [C3], C-term (NBP1-31098) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp1-31098_rabbit-polyclonal-importin-alpha-2-kpna2-antibody-immunocytochemistry-immunofluorescence-141120251844345.jpg)
![Western Blot: Importin alpha 2/KPNA2 Antibody [NBP1-76298] - Western blot analysis of KPNA2 expression in Daudi cell lysate with KPNA2 antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Western-Blot-NBP1-76298-img0005.jpg "Western Blot: Importin alpha 2/KPNA2 Antibody [NBP1-76298] - Western blot analysis of KPNA2 expression in Daudi cell lysate with KPNA2 antibody at (A) 1 and (B) 2 ug/ml.")
![Western Blot: Importin alpha 2/KPNA2 Antibody [NBP1-76298] - Rat heart tissue lysate with KPNA2 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Western-Blot-NBP1-76298-img0001.jpg)
![Immunohistochemistry: Importin alpha 2/KPNA2 Antibody [NBP1-76298] - Immunohistochemistry of KPNA2 in human heart tissue with KPNA2 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunohistochemistry-NBP1-76298-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: Importin alpha 2/KPNA2 Antibody - BSA Free [NBP1-76298] - Immunofluorescence of Importin alpha 2/KPNA2 in human heart tissue with Importin alpha 2/KPNA2 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-76298_rabbit-polyclonal-importin-alpha-2-kpna2-antibody-2711202419432251.jpg)
![Western Blot: RPL3 Antibody [NBP1-81332] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/RPL3-Antibody-Western-Blot-NBP1-81332-img0005.jpg "Western Blot: RPL3 Antibody [NBP1-81332] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunocytochemistry/Immunofluorescence: RPL3 Antibody [NBP1-81332] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RPL3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81332-img0006.jpg)
![Immunohistochemistry-Paraffin: RPL3 Antibody [NBP1-81332] - Staining of human cerebral cortex shows strong cytoplasmic and nuclear(nucleolar) positivity in neuronal cells.](http://images.novusbio.com/fullsize/RPL3-Antibody-Immunohistochemistry-Paraffin-NBP1-81332-img0002.jpg)
![Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody [NBP1-82582] - Staining of human lymph node shows high expression.](http://images.novusbio.com/fullsize/NFATC2-NFAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-82582-img0007.jpg "Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody [NBP1-82582] - Staining of human lymph node shows high expression.")
![Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody [NBP1-82582] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/NFATC2-NFAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-82582-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody [NBP1-82582] - Staining in human lymph node and cerebral cortex tissues using anti-NFATC2 antibody. Corresponding NFATC2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NFATC2-NFAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-82582-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: RIPK3/RIP3 Antibody (1312D) [NBP2-76799] - RIPK3/RIP3 was detected in immersion fixed SK-BR-3 human breast cancer cell line (positive stain) and HepG2 human human hepatocellular carcinoma cell line (negative stain) using Rabbit Monoclonal Anti-Human RIPK3/RIP3 Antibody [NBP2-76799] at 1 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; [NL004]) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-1312D-Immunocytochemistry-Immunofluorescence-NBP2-76799-img0001.jpg "Immunocytochemistry/Immunofluorescence: RIPK3/RIP3 Antibody (1312D) [NBP2-76799] - RIPK3/RIP3 was detected in immersion fixed SK-BR-3 human breast cancer cell line (positive stain) and HepG2 human human hepatocellular carcinoma cell line (negative stain) using Rabbit Monoclonal Anti-Human RIPK3/RIP3 Antibody [NBP2-76799] at 1 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; [NL004]) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.")
![Western Blot: FXR1 Antibody (PSH01-35) [NBP3-32355] - Western blot analysis of FXR1 on different lysates with Rabbit anti-FXR1 antibody (NBP3-32355) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: HepG2 cell lysate Lane 4: A549 cell lysate Lane 5: K-562 cell lysate Lane 6: COS-1 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: C2C12 cell lysate Lane 9: PC-12 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 70 kDa Observed band size: 70/75 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32355) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32355_rabbit-fxr1-mab-psh01-35-278202415295525.jpg "Western Blot: FXR1 Antibody (PSH01-35) [NBP3-32355] - Western blot analysis of FXR1 on different lysates with Rabbit anti-FXR1 antibody (NBP3-32355) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: HepG2 cell lysate Lane 4: A549 cell lysate Lane 5: K-562 cell lysate Lane 6: COS-1 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: C2C12 cell lysate Lane 9: PC-12 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 70 kDa Observed band size: 70/75 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32355) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: FXR1 Antibody (PSH01-35) [NBP3-32355] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-FXR1 antibody (NBP3-32355) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32355) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32355_rabbit-fxr1-mab-psh01-35-29820241435234.jpg)
![Immunocytochemistry/ Immunofluorescence: FXR1 Antibody (PSH01-35) [NBP3-32355] - Immunocytochemistry analysis of PC-12 cells labeling FXR1 with Rabbit anti-FXR1 antibody (NBP3-32355) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FXR1 antibody (NBP3-32355) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32355_rabbit-fxr1-mab-psh01-35-2920241192383.jpg)
![Western Blot: Importin alpha 2/KPNA2 Antibody [NB100-1371] - Staining of Hela lysate (RIPA buffer, 35 ug total protein per lane). Antibody at 0.03 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Western-Blot-NB100-1371-img0006.jpg "Western Blot: Importin alpha 2/KPNA2 Antibody [NB100-1371] - Staining of Hela lysate (RIPA buffer, 35 ug total protein per lane). Antibody at 0.03 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody [NB100-1371] - Staining of paraffin embedded Human Testis. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunohistochemistry-Paraffin-NB100-1371-img0007.jpg)
![Western Blot: Histamine H1 R Antibody [NBP1-06039] - (0.5ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Histamine-H1-R-Antibody-Western-Blot-NBP1-06039-img0003.jpg "Western Blot: Histamine H1 R Antibody [NBP1-06039] - (0.5ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Histamine H1 R Antibody [NBP1-06039] - (4ug/ml) staining of paraffin embedded Human Lung. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/Histamine-H1-R-Antibody-Immunohistochemistry-Paraffin-NBP1-06039-img0004.jpg)