| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56878SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44025 | |||
| BSA Free | NBP2-52909-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-31866-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-71691SS | |||
![Western Blot: RFX1 Antibody [NBP1-52654] - Detection of Human RFX1 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFX1 antibody NBP1-52654 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/RFX1-Antibody-Western-Blot-NBP1-52654-img0006.jpg "Western Blot: RFX1 Antibody [NBP1-52654] - Detection of Human RFX1 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFX1 antibody NBP1-52654 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry: RFX1 Antibody [NBP1-52654] - Section stained with anti-RFX1 antibody. RFX1 belongs to Cluster 4. Spermatids were stained in brown. These distribution patterns were mostly consisted to that of trancripts. (Abbreviation: SPT=spermatid).Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26753906/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RFX1-Antibody-Immunohistochemistry-NBP1-52654-img0008.jpg)
![Immunohistochemistry-Paraffin: RFX1 Antibody [NBP1-52654] - Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- RFX1 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/RFX1-Antibody-Immunohistochemistry-Paraffin-NBP1-52654-img0004.jpg)
![Immunohistochemistry-Paraffin: RFX1 Antibody [NBP1-52654] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RFX1 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/RFX1-Antibody-Immunohistochemistry-Paraffin-NBP1-52654-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: RFX1 Antibody [NBP1-52654] - Detection of human RFX1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RFX1 antibody NBP1-52654 used for IP at 6 ug per reaction. RFX1 was also immunoprecipitated by another rabbit anti-RFX1 antibody. For blotting immunoprecipitated RFX1, NBP1-52654 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/RFX1-Antibody-Immunoprecipitation-NBP1-52654-img0007.jpg)
![Western Blot: NOD1 Antibody [NB100-56878] - analysis of NOD1 in (A) recombinant fusion protein containing amino acids 930-949 and (B) fusion partner without these amino acids, using NOD1 antibody at 1:2500.](http://images.novusbio.com/fullsize/NOD1-Antibody-Western-Blot-NB100-56878-img0003.jpg "Western Blot: NOD1 Antibody [NB100-56878] - analysis of NOD1 in (A) recombinant fusion protein containing amino acids 930-949 and (B) fusion partner without these amino acids, using NOD1 antibody at 1:2500.")
![Immunohistochemistry-Paraffin: NOD1 Antibody [NB100-56878] - analysis of human pancreas using NOD1 antibody at 1:100.](http://images.novusbio.com/fullsize/NOD1-Antibody-Immunohistochemistry-Paraffin-NB100-56878-img0002.jpg)
![Western Blot: TrkA Antibody (JJ084-04) [NBP2-67473] - Analysis of TrkA on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Western-Blot-NBP2-67473-img0010.jpg "Western Blot: TrkA Antibody (JJ084-04) [NBP2-67473] - Analysis of TrkA on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: TrkA Antibody (JJ084-04) [NBP2-67473] - Staining TrkA in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Immunocytochemistry-Immunofluorescence-NBP2-67473-img0008.jpg)
![Immunohistochemistry-Paraffin: TrkA Antibody (JJ084-04) [NBP2-67473] - Analysis of paraffin-embedded mouse liver tissue using anti-TrkA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Immunohistochemistry-Paraffin-NBP2-67473-img0006.jpg)
![Flow Cytometry: TrkA Antibody (JJ084-04) [NBP2-67473] - Analysis of SH-SY-5Y cells with TrkA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Flow-Cytometry-NBP2-67473-img0001.jpg)
![Western Blot: TrkA Antibody (JJ084-04) [NBP2-67473] - Analysis of TrkA on different lysates using anti-TrkA antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse brain Lane 2: SH-SY-5Y](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Western-Blot-NBP2-67473-img0007.jpg)
![Western Blot: TrkA Antibody (JJ084-04) [NBP2-67473] - Western blot analysis of TrkA on N2A cell lysates using anti-TrkA antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Western-Blot-NBP2-67473-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: TrkA Antibody (JJ084-04) [NBP2-67473] - Staining TrkA in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Immunocytochemistry-Immunofluorescence-NBP2-67473-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: TrkA Antibody (JJ084-04) [NBP2-67473] - Staining TrkA in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Immunocytochemistry-Immunofluorescence-NBP2-67473-img0003.jpg)
![Immunohistochemistry-Paraffin: TrkA Antibody (JJ084-04) [NBP2-67473] - Analysis of paraffin-embedded human tonsil tissue using anti-TrkA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Immunohistochemistry-Paraffin-NBP2-67473-img0004.jpg)
![Immunohistochemistry-Paraffin: TrkA Antibody (JJ084-04) [NBP2-67473] - Analysis of paraffin-embedded mouse kidney tissue using anti-TrkA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Immunohistochemistry-Paraffin-NBP2-67473-img0005.jpg)
![Western Blot: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of MHC class I on different lysates with Rabbit anti-MHC class I antibody at 1/2,000 dilution.Lane 1: HL-60 cell lysateLane 2: K-562 cell lysate (low expression)Lysates/proteins at 15 µg/Lane.Predicted band size: 41 kDaObserved band size: 41 kDaExposure time: 5 minutes;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4c overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-66946_rabbit-monoclonal-mhc-class-i-antibody-jf10-38-western-blot-362024112212..jpg "Western Blot: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of MHC class I on different lysates with Rabbit anti-MHC class I antibody at 1/2,000 dilution.Lane 1: HL-60 cell lysateLane 2: K-562 cell lysate (low expression)Lysates/proteins at 15 µg/Lane.Predicted band size: 41 kDaObserved band size: 41 kDaExposure time: 5 minutes;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4c overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-MHC class I antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-66946_rabbit-monoclonal-mhc-class-i-antibody-jf10-38-immunohistochemistry-paraffin-362024112318..jpg)
![Immunohistochemistry-Paraffin: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of paraffin-embedded human liver cancer tissue with Rabbit anti-MHC class I antibody at 1/200 dilution.](http://images.novusbio.com/fullsize/nbp2-66946_rabbit-monoclonal-mhc-class-i-antibody-jf10-38-immunohistochemistry-paraffin-362024112412..jpg)
![Immunohistochemistry-Paraffin: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of paraffin-embedded human tonsil tissue with Rabbit anti-MHC class I antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-66946_rabbit-monoclonal-mhc-class-i-antibody-jf10-38-immunohistochemistry-paraffin-362024112523..jpg)
![Immunohistochemistry-Paraffin: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of paraffin-embedded human pancreas tissue with Rabbit anti-MHC class I antibody at 1/200 dilution.](http://images.novusbio.com/fullsize/nbp2-66946_rabbit-monoclonal-mhc-class-i-antibody-jf10-38-immunohistochemistry-paraffin-362024112620..jpg)
![Flow Cytometry: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of HL-60 cells labeling MHC class I.Cells were fixed and permeabilized. Then stained with the primary antibody (1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4c for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4c. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp2-66946_rabbit-monoclonal-mhc-class-i-antibody-jf10-38-flow-cytometry-362024112742..jpg)
![Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of paraffin-embedded human tonsil tissue labeling MHC class I with Rabbit anti-MHC class I antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/200 dilution overnight at 4 c, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-66946_rabbit-monoclonal-mhc-class-i-antibody-jf10-38-immunocytochemistry-immunofluorescence-362024112932..jpg)
![Western Blot: CTCF Antibody (CL0304) [NBP2-52909] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-CTCF antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0304-Western-Blot-NBP2-52909-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CTCF Antibody (CL0304) [NBP2-52909] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-CTCF antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CTCF Antibody (CL0304) [NBP2-52909] - Staining of MCF7 cells using the Anti-CTCF monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0304-Immunocytochemistry-Immunofluorescence-NBP2-52909-img0011.jpg)
![Immunohistochemistry-Paraffin: CTCF Antibody (CL0304) [NBP2-52909] - Staining of human testis shows moderate nuclear positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0304-Immunohistochemistry-Paraffin-NBP2-52909-img0009.jpg)
![Western Blot: CTCF Antibody (CL0304) [NBP2-52909] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0304-Western-Blot-NBP2-52909-img0004.jpg)
![Immunohistochemistry-Paraffin: CTCF Antibody (CL0304) [NBP2-52909] - Staining of human endometrium shows moderate to strong nuclear positivity in glandular and stromal cells.](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0304-Immunohistochemistry-Paraffin-NBP2-52909-img0006.jpg)
![Immunohistochemistry-Paraffin: CTCF Antibody (CL0304) [NBP2-52909] - Staining of human kidney shows moderate to strong nuclear positivity in cells in tubules and glomeruli.](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0304-Immunohistochemistry-Paraffin-NBP2-52909-img0007.jpg)
![Immunohistochemistry-Paraffin: CTCF Antibody (CL0304) [NBP2-52909] - Staining of human small intestine shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0304-Immunohistochemistry-Paraffin-NBP2-52909-img0008.jpg)
![Immunohistochemistry-Paraffin: ULBP-3 Antibody [NBP2-31866] - Staining of human testis shows moderate positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/ULBP-3-Antibody-Immunohistochemistry-Paraffin-NBP2-31866-img0014.jpg "Immunohistochemistry-Paraffin: ULBP-3 Antibody [NBP2-31866] - Staining of human testis shows moderate positivity in a subset of cells in seminiferous ducts.")
![Immunocytochemistry/Immunofluorescence: ULBP-3 Antibody [NBP2-31866] - Staining ULBP-3 in methanol-fixed, Jurkat cells using anti-ULBP-3 antibody. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ULBP-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-31866-img0013.jpg)
![Immunohistochemistry-Paraffin: ULBP-3 Antibody [NBP2-31866] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-31866_rabbit-polyclonal-ulbp-3-antibody-immunohistochemistry-paraffin-313202511383..png)
![Immunohistochemistry-Paraffin: ULBP-3 Antibody [NBP2-31866] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-31866_rabbit-polyclonal-ulbp-3-antibody-immunohistochemistry-paraffin-3132025113854..png)
![Immunohistochemistry-Paraffin: ULBP-3 Antibody [NBP2-31866] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-31866_rabbit-polyclonal-ulbp-3-antibody-immunohistochemistry-paraffin-313202511406..png)
![Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody (3F2C2) [NB110-60512] - Analysis using GSTP1 mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY GSTP1 cDNA (2).](http://images.novusbio.com/fullsize/Glutathione-S-Transferase-pi-1-GSTP1-Antibody-3F2C2-Western-Blot-NB110-60512-img0023.jpg "Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody (3F2C2) [NB110-60512] - Analysis using GSTP1 mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY GSTP1 cDNA (2).")
![Immunocytochemistry/Immunofluorescence: Glutathione S-Transferase pi 1/GSTP1 Antibody (3F2C2) [NB110-60512] - Analysis of HepG2 (left) and L-02 (right) cells using GSTP1 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/Glutathione-S-Transferase-pi-1-GSTP1-Antibody-3F2C2-Immunocytochemistry-Immunofluorescence-NB110-60512-img0024.jpg)
![Immunohistochemistry-Paraffin: Glutathione S-Transferase pi 1/GSTP1 Antibody (3F2C2) [NB110-60512] - Analysis of human prostate tissues using GSTP1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Glutathione-S-Transferase-pi-1-GSTP1-Antibody-3F2C2-Immunohistochemistry-Paraffin-NB110-60512-img0021.jpg)
![Flow Cytometry: Glutathione S-Transferase pi 1/GSTP1 Antibody (3F2C2) [NB110-60512] - Analysis of K562 cells using GSTP1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Glutathione-S-Transferase-pi-1-GSTP1-Antibody-3F2C2-Flow-Cytometry-NB110-60512-img0014.jpg)
![Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody (3F2C2) [NB110-60512] - Analysis using GSTP1 mouse mAb against PC3 cell lysate (1) and human cerebellum tissue lysate (2).](http://images.novusbio.com/fullsize/Glutathione-S-Transferase-pi-1-GSTP1-Antibody-3F2C2-Western-Blot-NB110-60512-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: Glutathione S-Transferase pi 1/GSTP1 Antibody (3F2C2) [NB110-60512] - Analysis of PC-3 cells using GSTP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/Glutathione-S-Transferase-pi-1-GSTP1-Antibody-3F2C2-Immunocytochemistry-Immunofluorescence-NB110-60512-img0013.jpg)
![Western Blot: IFIT2 Antibody [NBP1-31164] - p115 and Sec16A are required for optimal IRF-3 activation in response to activation of cytosolic RNA and DNA sensors.HeLa cells were infected with lentiviral vectors encoding shRNA targeting p115. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1002747) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IFIT2-Antibody-Western-Blot-NBP1-31164-img0006.jpg "Western Blot: IFIT2 Antibody [NBP1-31164] - p115 and Sec16A are required for optimal IRF-3 activation in response to activation of cytosolic RNA and DNA sensors.HeLa cells were infected with lentiviral vectors encoding shRNA targeting p115. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1002747) licensed under a CC-BY license.")
![Western Blot: IFIT2 Antibody [NBP1-31164] - Sample(30 ug of whole cell lysate)A:A431 B:Hep G2 7.5% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/IFIT2-Antibody-Western-Blot-NBP1-31164-img0003.jpg)
![Western Blot: IFIT2 Antibody [NBP1-31164] - p115 & Sec16A are required for optimal IRF-3 activation in response to activation of cytosolic RNA & DNA sensors.HeLa cells were infected with lentiviral vectors encoding shRNA targeting p115 (A) or Sec16A (B) & non-targeting (NT) control shRNA for 24 h followed by puromycin selection (1.5 µg/ml) for 5 days. Cells were left untreated or stimulated with poly I:C (1 µg/ml), poly dA:dT (1 µg/ml) or SeV (200 HAU/ml) for 16 h. Whole-cell lysates were prepared & subjected to immunoblot analysis with indicated antibodies. One of two independent experiments with similar results is shown. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22792062), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31164_rabbit-polyclonal-ifit2-antibody-310202415171321.jpg)
![IFIT2 antibody [N1], N-term detects IFIT2 protein at endoplasmic reticulum by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: IFIT2 protein stained by IFIT2 antibody [N1], N-term (NBP1-31164) diluted at 1:200.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-31164_rabbit-polyclonal-ifit2-antibody-immunocytochemistry-immunofluorescence-1411202518351210.jpg)
![Western Blot: B7-H3/CD276 Antibody [NBP3-14669] - Samples: Whole cell lysate (50 ug) from TCMK-1, NIH 3T3,AlphaTC1 Clone 9, 3T3-L1, and CT26 cells prepared usingNETN lysis buffer. Antibody: Rabbit anti-B7-H3/CD276recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goatanti-rabbit IgG. Chemiluminescence with anexposure time of 3 minutes.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Western-Blot-NBP3-14669-img0002.jpg "Western Blot: B7-H3/CD276 Antibody [NBP3-14669] - Samples: Whole cell lysate (50 ug) from TCMK-1, NIH 3T3,AlphaTC1 Clone 9, 3T3-L1, and CT26 cells prepared usingNETN lysis buffer. Antibody: Rabbit anti-B7-H3/CD276recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goatanti-rabbit IgG. Chemiluminescence with anexposure time of 3 minutes.")
![Immunocytochemistry/Immunofluorescence: B7-H3/CD276 Antibody (BLR025F) [NBP3-14669] - Section of A-172 cells. Antibody: Rabbit anti-B7-H3/CD276 recombinant monoclonal used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunohistochemistry-NBP3-14669-img0005.jpg)
![Immunohistochemistry: B7-H3/CD276 Antibody [NBP3-14669] - Sample: FFPE section of humanlung carcinoma. Antibody: Rabbit anti-B7-H3/CD276recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat antirabbit IgG. Substrate: Opal™. Counterstain:DAPI (blue).](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunohistochemistry-NBP3-14669-img0006.jpg)
![Western Blot: B7-H3/CD276 Antibody [NBP3-14669] - Rabbit anti CD276 recombinant monoclonal antibody (NBP3-14669 lot 1) used at 1:1000. HRP-conjugated goat anti-rabbit IgG (NB7156). Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-GAPDH (NB300-322).](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Western-Blot-NBP3-14669-img0001.jpg)
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody (BLR025F) [NBP3-14669] - Section of ovarian carcinoma. Antibody: Rabbit anti-B7-H3/CD276 recombinant monoclonal antibody used at 1:200. Secondary: HRP-conjugated goat antirabbit IgG (A120-501P). Substrate: DAB.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunohistochemistry-NBP3-14669-img0004.jpg)
![Immunoprecipitation: B7-H3/CD276 Antibody [NBP3-14669] - Samples: Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from HeLa cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-B7-H3/CD276 recombinant monoclonal antibodyused for IP at 3 ul per reaction. B7-H3/CD276 was also immunoprecipitated by rabbit anti-B7-H3/CD276 recombinant monoclonal antibody. For blotting immunoprecipitated B7-H3/CD27 was used at 1:1000.Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunoprecipitation-NBP3-14669-img0003.jpg)
![B7-H3/CD276 Antibody (BLR025F) [NBP3-14669] - Detection of human B7-H3/CD276 (shaded) in 293T cells by flow cytometry. Antibody: Rabbit antiB7-H3/CD276 recombinant monoclonal [BLR025F]or isotype control (unshaded).Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-BLR025F-NBP3-14669-img0010.jpg)
![Western Blot: Dicer Antibody (HL1232) - Azide and BSA Free [NBP3-25439] - Non-transfected (-) and transfected (+) MCF-7 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (NBP3-25439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25439_rabbit-dicer-mab-hl1232-azide-and-bsa-free-2120249403838.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Dicer Antibody (HL1232) - Azide and BSA Free [NBP3-25439] - Non-transfected (-) and transfected (+) MCF-7 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (NBP3-25439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: Dicer Antibody (HL1232) - Azide and BSA Free [NBP3-25439] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (NBP3-25439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25439_rabbit-dicer-mab-hl1232-azide-and-bsa-free-2120249393545.jpg)
![Western Blot: Dicer Antibody (HL1232) - Azide and BSA Free [NBP3-25439] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (NBP3-25439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25439_rabbit-dicer-mab-hl1232-azide-and-bsa-free-2120249432868.jpg)
![Immunocytochemistry/Immunofluorescence: Dicer Antibody (HL1232) - Azide and BSA Free [NBP3-25439] - Dicer antibody [HL1232] detects Dicer protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dicer stained by Dicer antibody [HL1232] (NBP3-25439) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-25439_rabbit-dicer-mab-hl1232-azide-and-bsa-free-2120249362777.jpg)
![Western Blot: Dicer Antibody (HL1232) - Azide and BSA Free [NBP3-25439] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (NBP3-25439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25439_rabbit-dicer-mab-hl1232-azide-and-bsa-free-212024939176.jpg)
![Immunohistochemistry-Paraffin: Dicer Antibody (HL1232) - Azide and BSA Free [NBP3-25439] - Dicer antibody [HL1232] detects Dicer protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Dicer stained by Dicer antibody [HL1232] (NBP3-25439) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25439_rabbit-dicer-mab-hl1232-azide-and-bsa-free-2120249371566.jpg)
![Western Blot: Dicer Antibody (HL1232) - Azide and BSA Free [NBP3-25439] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (NBP3-25439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25439_rabbit-dicer-mab-hl1232-azide-and-bsa-free-2120249362730.jpg)
![Immunocytochemistry/Immunofluorescence: Dicer Antibody [NBP1-71691] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Dicer at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-71691-img0008.jpg "Immunocytochemistry/Immunofluorescence: Dicer Antibody [NBP1-71691] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Dicer at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: Dicer Antibody [NBP1-71691] - Analysis of Dicer in paraffin embedded human colorectal cancer tissue using anti-Dicer antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunohistochemistry-Paraffin-NBP1-71691-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Dicer Antibody [NBP1-71691] - Dicer antibody was tested in HeLa cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-71691-img0005.jpg)
![Immunohistochemistry: Dicer Antibody [NBP1-71691] - Staining of Dicer in mouse prostate using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunohistochemistry-NBP1-71691-img0006.jpg)
![Western Blot: NOX1 Antibody [NBP1-69573] - NOX1 antibody - C-terminal region validated by WB using Epithelial Colorectal Adenocarcinoma CaCO2 at 1:10.](http://images.novusbio.com/fullsize/NOX1-Antibody-Western-Blot-NBP1-69573-img0003.jpg "Western Blot: NOX1 Antibody [NBP1-69573] - NOX1 antibody - C-terminal region validated by WB using Epithelial Colorectal Adenocarcinoma CaCO2 at 1:10.")
![Western Blot: NOX1 Antibody [NBP1-69573] - This Anti-NOX1 antibody was used in Western Blot of Jurkat tissue lysate at a concentration of 0.5ug/ml.](http://images.novusbio.com/fullsize/NOX1-Antibody-Western-Blot-NBP1-69573-img0002.jpg)