| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Low Endotoxin, Azide and BSA Free | NBP2-75925SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85807-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27224 | |||
| BSA Free | NBP2-27223SS | |||
| Azide and BSA Free | NBP2-27224SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44541 | |||
| Azide and BSA Free | NBP2-34587-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18333-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37871-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44491 | |||
| BSA Free | NBP2-34482-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47675-0.1mg | |||
| IHC-Prediluted | NBP2-48470 | |||
![Western Blot: TNF-alpha Antibody (2342C) - Low Endotoxin and Azide Free [NBP2-75925] - Western blot shows conditioned media from CHO Chinese hamster ovary cell line either mock transfected or transfected with human TNF- alpha. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human TNF-alpha Monoclonal Antibody (NBP2-75925) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for TNF-alpha at approximately 18 and 21 kDa (as indicated). This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-2342C-Low-Endotoxin-and-Azide-Free-Western-Blot-NBP2-75925-img0002.jpg "Western Blot: TNF-alpha Antibody (2342C) - Low Endotoxin and Azide Free [NBP2-75925] - Western blot shows conditioned media from CHO Chinese hamster ovary cell line either mock transfected or transfected with human TNF- alpha. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human TNF-alpha Monoclonal Antibody (NBP2-75925) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for TNF-alpha at approximately 18 and 21 kDa (as indicated). This experiment was conducted under reducing conditions.")
![Immunohistochemistry-Paraffin: TNF-alpha Antibody (2342C) - Low Endotoxin and Azide Free [NBP2-75925] - Analysis of a FFPE tissue section of the human intesting using 1:200 dilution of TNF-alpha antibody (NBP2-75925). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated a moderate to strong cytoplasmic staining in crypt cells.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-2342C-Low-Endotoxin-and-Azide-Free-Immunohistochemistry-Paraffin-NBP2-75925-img0003.jpg)
![Neutralization: TNF-alpha Antibody (2342C) [NBP2-75925] - Recombinant Human TNF-alpha (Catalog # 210-TA) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Cytotoxicity elicited by Recombinant Human TNF-alpha (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF-alpha Monoclonal Antibody (Catalog # NBP2-75925). The ND50 is typically 1-10 ng/mL in the presence of the metabolic inhibitor actinomycin D.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-2342C-Neutralization-NBP2-75925-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Cathepsin S Antibody [NBP1-85807] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Cathepsin-S-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85807-img0007.jpg "Immunocytochemistry/Immunofluorescence: Cathepsin S Antibody [NBP1-85807] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Cathepsin S Antibody [NBP1-85807] -Staining of human lymph node shows strong cytoplasmic granular positivity in a subset of non-germinal center cells.and germinal center cells](http://images.novusbio.com/fullsize/antibody/nbp1-85807_rabbit-polyclonal-cathepsin-s-antibody-immunohistochemistry-paraffin-2132024113128..jpg)
![Immunohistochemistry-Paraffin: Cathepsin S Antibody [NBP1-85807] -Staining of human colon shows strong cytoplasmic granular positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-85807_rabbit-polyclonal-cathepsin-s-antibody-immunohistochemistry-paraffin-2132024113455..jpg)
![Immunohistochemistry-Paraffin: beta-2 Adrenergic R/ADRB2 Antibody (JM102-06) [NBP2-67187] - Analysis of paraffin-embedded mouse liver tissue using anti-ADRB2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/beta-2-Adrenergic-R-ADRB2-Antibody-JM102-06-Immunohistochemistry-Paraffin-NBP2-67187-img0002.jpg "Immunohistochemistry-Paraffin: beta-2 Adrenergic R/ADRB2 Antibody (JM102-06) [NBP2-67187] - Analysis of paraffin-embedded mouse liver tissue using anti-ADRB2 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: beta-2 Adrenergic R/ADRB2 Antibody (JM102-06) [NBP2-67187] - Analysis of paraffin-embedded mouse brain tissue using anti-ADRB2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/beta-2-Adrenergic-R-ADRB2-Antibody-JM102-06-Immunohistochemistry-Paraffin-NBP2-67187-img0001.jpg)
![Western Blot: beta-2 Adrenergic R/ADRB2 Antibody (JM102-06) [NBP2-67187] - Analysis of beta 2 Adrenergic Receptor on different lysates with Rabbit anti-beta 2 Adrenergic Receptor antibody at 1/5,000 dilution.Lane 1: A431 cell lysate (15 µg/Lane)Lane 2: Human liver tissue lysate (20 µg/Lane)Lane 3: Mouse heart tissue lysate (20 µg/Lane)Lane 4: Rat heart tissue lysate (20 µg/Lane)Lane 5: Mouse kidney tissue lysate (20 µg/Lane)Lane 6: Rat kidney tissue lysate (20 µg/Lane)Predicted band size: 46 kDaObserved band size: 55-100 kDaExposure time: 3 minutes; ECL: K1801;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67187_rabbit-monoclonal-beta-2-adrenergic-r-adrb2-antibody-jm102-06-western-blot-28620249199..jpg)
![Western Blot: beta-2 Adrenergic R/ADRB2 Antibody (JM102-06) [NBP2-67187] -Analysis of beta 2 Adrenergic Receptor on different lysates with Rabbit anti-beta 2 Adrenergic Receptor antibody at 1/1,000 dilution.Lane 1: A431 cell lysate (no heat) (15 µg/Lane)Lane 2: Mouse kidney tissue lysate (no heat) (20 µg/Lane)Lane 3: Mouse heart tissue lysate (no heat) (20 µg/Lane)Lane 4: Rat kidney tissue lysate (no heat) (20 µg/Lane)Lane 5: Zebrafish tissue lysate (20 µg/Lane)Predicted band size: 46 kDaObserved band size: 55-100 kDaExposure time: 3 minutes; ECL: K1801;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-04) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67187_rabbit-monoclonal-beta-2-adrenergic-r-adrb2-antibody-jm102-06-western-blot-286202492022..jpg)
![Flow Cytometry-beta-2 Adrenergic R/ADRB2 Antibody (JM102-06) [NBP2-67187] -Analysis of A431 cells labeling beta 2 Adrenergic Receptor.Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp2-67187_rabbit-monoclonal-beta-2-adrenergic-r-adrb2-antibody-jm102-06-flow-cytometry-28620249222..jpg)
![ICC/IF-beta-2 Adrenergic R/ADRB2 Antibody (JM102-06) [NBP2-67187] - Analysis of A431 cells labeling beta 2 Adrenergic Receptor with Rabbit anti-beta 2 Adrenergic Receptor antibody at 1/100 dilution.Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-beta 2 Adrenergic Receptor antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin ( red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-67187_rabbit-monoclonal-beta-2-adrenergic-r-adrb2-antibody-jm102-06-immunocytochemistry-immunofluorescence-286202492524..jpg)
![Western Blot: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Analysis using Azide Free version of NBP2-27223. TNF alpha recombinant protein using TNF alpha antibody at 3 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-6N1E7-Western-Blot-NBP2-27223-img0004.jpg "Western Blot: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Analysis using Azide Free version of NBP2-27223. TNF alpha recombinant protein using TNF alpha antibody at 3 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Human PBMC were stimulated with PMA (10 ng/mL) and Ionomycin (500 ng/mL) for 5h in presence of Brefeldin A. At the end of stimulation, cells were surface stained before testing by intracellular staining. Fixed and permeabilized cells were incubated with 10 uL (0.5 ug) of TNF- alpha antibody for 30 minutes on ice. Lymphocyte population was gated for analysis to show TNF alpha positive T cells.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-6N1E7-Flow-Cytometry-NBP2-27223-img0002.jpg)
![Inhibition Activity: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Validation of inhibitory effects of TNF alpha antibody on TNF alpha activity in the cell line. NF-kB/SEAPorter cells were plated in 96-well plate at 5 x 10^4 cells per well for 16 h. Cells were pre-incubated with TNF alpha antibody at 10 and 20 ug/ml for 1 hr, then treated with 10 ng/ml TNF alpha or 10 ng/ml PMA. After 24h, the secreted alkaline phosphatase (SEAP) was analyzed using the SEAPorterAssay Kit . Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-6N1E7-Inhibition-Activity-NBP2-27223-img0006.jpg)
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (C68/684) [NBP2-32832] - Formalin-fixed, paraffin-embedded human Tonsil stained with .](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-C68-684-Immunohistochemistry-Paraffin-NBP2-32832-img0006.jpg "Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (C68/684) [NBP2-32832] - Formalin-fixed, paraffin-embedded human Tonsil stained with .")
![Western Blot: CD68/SR-D1 Antibody (C68/684) [NBP2-32832] - Western Blot Analysis of human Spleen tissue lysate using CD68/SR-D1 Antibody (C68/684).](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-C68-684-Western-Blot-NBP2-32832-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CD68/SR-D1 Antibody (C68/684) [NBP2-32832] - Immunofluorescence staining of U87MG cells using CD68/SR-D1 Antibody (C68/684) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-C68-684-Immunocytochemistry-Immunofluorescence-NBP2-32832-img0004.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (C68/684) [NBP2-32832] - Flow Cytometric Analysis of U87MG cells using CD68/SR-D1 Antibody (C68/684).followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-C68-684-Flow-Cytometry-NBP2-32832-img0008.jpg)
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (C68/684) [NBP2-32832] - Formalin-fixed, paraffin-embedded human Histiocytoma stained with CD68/SR-D1 Antibody (C68/684).](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-C68-684-Immunohistochemistry-Paraffin-NBP2-32832-img0005.jpg)
![Immunohistochemistry-Paraffin: TREM2 Antibody (HL1738) - Azide and BSA Free [NBP3-25728] - TREM2 antibody [HL1738] detects TREM2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. TREM2 stained by TREM2 antibody [HL1738] (NBP3-25728) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-212024940820.jpg "Immunohistochemistry-Paraffin: TREM2 Antibody (HL1738) - Azide and BSA Free [NBP3-25728] - TREM2 antibody [HL1738] detects TREM2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. TREM2 stained by TREM2 antibody [HL1738] (NBP3-25728) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunocytochemistry/Immunofluorescence: TREM2 Antibody (HL1738) - Azide and BSA Free [NBP3-25728] - TREM2 antibody [HL1738] detects TREM2 protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TREM2 stained by TREM2 antibody [HL1738] (NBP3-25728) diluted at 1:250. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-2120249425467.jpg)
![Western Blot: TREM2 Antibody (HL1738) - Azide and BSA Free [NBP3-25728] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with TREM2 antibody [HL1738] (NBP3-25728) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-2120249422617.jpg)
![Sandwich ELISA detection of recombinant E.coli expressed, full-length human TREM2 protein using antibodies as below. Capture: TREM2 antibody [HL1739] (1 ug/mL)Please notice that NBP3-25728 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637387. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-elisa-1411202518443414.jpg)
![Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human TREM2 protein (1215.95-19.07 pM). Coated protein was probed with TREM2 antibody [HL1738] (NBP3-25728) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-elisa-1411202518414124.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with TREM2 antibody [HL1738] (NBP3-25728) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-western-blot-14112025184488.jpg)
![Sandwich ELISA detection of recombinant E.coli expressed, full-length human TREM2 protein using antibodies as below. Capture: TREM2 antibody [HL1738] (NBP3-25728) (5 ug/mL) Detection: HRP-conjugated TREM2 antibody [HL1739] (1 ug/mL)Please notice that GTX637387 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25728. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-elisa-141120251843335.jpg)
![Western Blot: MCPIP1/ZC3H12A Antibody [NBP3-18333] - WB Validation in Human Lung. Loading: 15 ug of lysate per lane. Antibodies: MCPIP1/ZC3H12A NBP3-18333, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution. Lane 1: 2 ug/mL Lane 2: 4 ug/mL](http://images.novusbio.com/fullsize/MCPIP1-ZC3H12A-Antibody-Western-Blot-NBP3-18333-img0001.jpg "Western Blot: MCPIP1/ZC3H12A Antibody [NBP3-18333] - WB Validation in Human Lung. Loading: 15 ug of lysate per lane. Antibodies: MCPIP1/ZC3H12A NBP3-18333, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution. Lane 1: 2 ug/mL Lane 2: 4 ug/mL")
![Western Blot: MCPIP1/ZC3H12A Antibody - BSA Free [NBP3-18333] - Validation in Mouse Tissues.Loading: 15 ug of lysates per lane. Antibodies: ZC3H12A , 2 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.](http://images.novusbio.com/fullsize/nbp3-18333_rabbit-mcpip1-zc3h12a-pab-2711202419241348.gif)
![Western Blot: MCPIP1/ZC3H12A Antibody [NBP3-18333] - Western Blot Validation in Rat Tissues. Loading: 15 ug of lysates per lane. Antibodies: MCPIP1/ZC3H12A NBP3-18333, 2 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.](http://images.novusbio.com/fullsize/MCPIP1-ZC3H12A-Antibody-Western-Blot-NBP3-18333-img0003.jpg)
![Immunohistochemistry: MCPIP1/ZC3H12A Antibody - BSA Free [NBP3-18333] - Validation of ZC3H12A in Human Testis. Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-ZC3H12A antibody at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp3-18333_rabbit-mcpip1-zc3h12a-pab-2711202419381765.jpg)
![Immunohistochemistry: MCPIP1/ZC3H12A Antibody - BSA Free [NBP3-18333] - Validation of ZC3H12A in Rat Brain. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ZC3H12A antibody at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp3-18333_rabbit-mcpip1-zc3h12a-pab-2711202419541514.jpg)
![Immunohistochemistry: MCPIP1/ZC3H12A Antibody - BSA Free [NBP3-18333] - Validation of ZC3H12A in Mouse Liver.Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ZC3H12A antibody at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin](http://images.novusbio.com/fullsize/nbp3-18333_rabbit-mcpip1-zc3h12a-pab-2711202419475869.jpg)
![Immunocytochemistry/Immunofluorescence: MCPIP1/ZC3H12A Antibody [NBP3-18333] - Immunofluorescent analysis of 4% paraformaldehyde-fixed THP-1 cells labeling MCPIP1/ZC3H12A with NBP3-18333 at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/MCPIP1-ZC3H12A-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-18333-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Plastin L Antibody [NBP2-19860] - Plastin L antibody detects Plastin L protein by immunofluorescent analysis. Sample: A549 cells were fixed in ice-cold MeOH for 5 min. Green: Plastin Lstained by Plastin L antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/Plastin-L-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19860-img0007.jpg "Immunocytochemistry/Immunofluorescence: Plastin L Antibody [NBP2-19860] - Plastin L antibody detects Plastin L protein by immunofluorescent analysis. Sample: A549 cells were fixed in ice-cold MeOH for 5 min. Green: Plastin Lstained by Plastin L antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.")
![Western Blot: Plastin L Antibody [NBP2-19860] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Plastin L antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Plastin-L-Antibody-Western-Blot-NBP2-19860-img0008.jpg)
![Immunohistochemistry-Paraffin: Plastin L Antibody [NBP2-19860] - Plastin L antibody detects Plastin L protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse thymus gland.Plastin L stained by Plastin L antibody (NBP2-19860) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19860_rabbit-polyclonal-plastin-l-antibody-91020242063320.jpg)
![Western Blot: MCPIP1/ZC3H12A Antibody [NBP2-37871] - Analysis in control (vector only transfected HEK293T lysate) and ZC3H12A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/MCPIP1-ZC3H12A-Antibody-Western-Blot-NBP2-37871-img0004.jpg "Western Blot: MCPIP1/ZC3H12A Antibody [NBP2-37871] - Analysis in control (vector only transfected HEK293T lysate) and ZC3H12A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: MCPIP1/ZC3H12A Antibody [NBP2-37871] - Staining of human cell line U-251 MG shows localization to cytoplasmic bodies. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MCPIP1-ZC3H12A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-37871-img0002.jpg)
![Immunohistochemistry-Paraffin: MCPIP1/ZC3H12A Antibody [NBP2-37871] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/MCPIP1-ZC3H12A-Antibody-Immunohistochemistry-Paraffin-NBP2-37871-img0008.jpg)
![Immunohistochemistry-Paraffin: MCPIP1/ZC3H12A Antibody [NBP2-37871] - Staining of human tonsil shows strong membranous positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/MCPIP1-ZC3H12A-Antibody-Immunohistochemistry-Paraffin-NBP2-37871-img0005.jpg)
![Immunohistochemistry-Paraffin: MCPIP1/ZC3H12A Antibody [NBP2-37871] - Staining of human colon shows moderate cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/MCPIP1-ZC3H12A-Antibody-Immunohistochemistry-Paraffin-NBP2-37871-img0006.jpg)
![Immunohistochemistry-Paraffin: MCPIP1/ZC3H12A Antibody [NBP2-37871] - Staining of human testis shows moderate cytoplasmic and nuclear positivity in Leydig cells.](http://images.novusbio.com/fullsize/MCPIP1-ZC3H12A-Antibody-Immunohistochemistry-Paraffin-NBP2-37871-img0007.jpg)
![Western Blot: CD68/SR-D1 Antibody [NBP2-48923] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-Western-Blot-NBP2-48923-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CD68/SR-D1 Antibody [NBP2-48923] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody [NBP2-48923] - Immunohistochemical staining of human lung shows strong cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-Immunohistochemistry-Paraffin-NBP2-48923-img0005.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody [NBP2-48923] - Staining in human tonsil and skeletal muscle tissues . Corresponding CD68 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-Immunohistochemistry-Paraffin-NBP2-48923-img0009.jpg)
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody [NBP2-48923] - Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in a subset of non-germinal center cells.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-Immunohistochemistry-Paraffin-NBP2-48923-img0003.jpg)
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody [NBP2-48923] - Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in tissue macrophages.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-Immunohistochemistry-Paraffin-NBP2-48923-img0004.jpg)
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody [NBP2-48923] - Immunohistochemical staining of human skeletal muscle shows no positivity as expected.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-Immunohistochemistry-Paraffin-NBP2-48923-img0002.jpg)
![Western Blot: CD68/SR-D1 Antibody (CL1346) [NBP2-34482] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1, using Anti-CD68 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-CL1346-Western-Blot-NBP2-34482-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CD68/SR-D1 Antibody (CL1346) [NBP2-34482] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1, using Anti-CD68 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: CD68/SR-D1 Antibody (CL1346) [NBP2-34482] - Staining of human cerebellum shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-CL1346-Immunohistochemistry-NBP2-34482-img0004.jpg)
![Western Blot: CD68/SR-D1 Antibody (CL1346) [NBP2-34482] - Lane 1: Marker [kDa]. Lane 2: Human spleen tissue lysate](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-CL1346-Western-Blot-NBP2-34482-img0005.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (CL1346) [NBP2-34482] - Staining of human tonsil shows strong immunoreactivity in a subset of lymphoid cells.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-CL1346-Immunohistochemistry-NBP2-34482-img0001.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (CL1346) [NBP2-34482] - Staining of human liver shows strong positivity in Kupffer cells.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-CL1346-Immunohistochemistry-NBP2-34482-img0002.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (CL1346) [NBP2-34482] - Staining of human colon shows strong immunoreactivity in a subset of lymphoid cells.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-CL1346-Immunohistochemistry-NBP2-34482-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: TNF-alpha Antibody (TNFA/1172) [NBP2-45331] - Immunofluorescence Analysis of PFA-fixed HePG2 cells labeling TNF-alpha with TNF-alpha Antibody (TNFA/1172) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-TNFA-1172-Immunocytochemistry-Immunofluorescence-NBP2-45331-img0007.jpg "Immunocytochemistry/Immunofluorescence: TNF-alpha Antibody (TNFA/1172) [NBP2-45331] - Immunofluorescence Analysis of PFA-fixed HePG2 cells labeling TNF-alpha with TNF-alpha Antibody (TNFA/1172) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.")
![Immunohistochemistry-Paraffin: TNF-alpha Antibody (TNFA/1172) [NBP2-45331] - Rat Stomach stained with TNF alpha Monoclonal Antibody (TNFA/1172)](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-TNFA-1172-Immunohistochemistry-Paraffin-NBP2-45331-img0005.jpg)
![Flow Cytometry: TNF-alpha Antibody (TNFA/1172) [NBP2-45331] - Flow Cytometric Analysis of PFA-fixed HePG2 cells using TNF-alpha Antibody (TNFA/1172).followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-TNFA-1172-Flow-Cytometry-NBP2-45331-img0006.jpg)
![Immunohistochemistry-Paraffin: TNF-alpha Antibody (TNFA/1172) [NBP2-45331] - Human Erdheim Chester disease (also known as polyostotic lerosing histiocytosis) stained with TNF alpha Monoclonal Antibody (TNFA/1172).](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-TNFA-1172-Immunohistochemistry-Paraffin-NBP2-45331-img0001.jpg)
![Immunohistochemistry-Paraffin: TNF-alpha Antibody (TNFA/1172) [NBP2-45331] - Rat Pancreas stained with TNF alpha Monoclonal Antibody (TNFA/1172)](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-TNFA-1172-Immunohistochemistry-Paraffin-NBP2-45331-img0004.jpg)
![Immunohistochemistry-Paraffin: TNF-alpha Antibody (TNFA/1172) [NBP2-45331] - Formalin-fixed, paraffin-embedded human Pancreas stained with TNF-alpha Antibody (TNFA/1172).](http://images.novusbio.com/fullsize/nbp2-45331_mouse-monoclonal-tnf-alpha-antibody-tnfa-1172-177202495945.jpg)