| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-116SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47911-0.1mg | |||
| IHC-Prediluted | NBP2-48363 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65900-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64849SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61794-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61871-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-296SS | |||
| Azide and BSA Free | NBP2-80763 | |||
![Immunocytochemistry/Immunofluorescence: ABCE1 Antibody [NB400-116] - ABCE1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/ABCE1-Antibody-Immunocytochemistry-Immunofluorescence-NB400-116-img0003.jpg "Immunocytochemistry/Immunofluorescence: ABCE1 Antibody [NB400-116] - ABCE1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).")
![Western Blot: ABCE1 Antibody [NB400-116] - WB analysis of ABCE1 in A431 lysate.](http://images.novusbio.com/fullsize/ABCE1-Antibody-Western-Blot-NB400-116-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ABCE1 Antibody [NB400-116] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-ABCE1 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ABCE1-Antibody-Immunocytochemistry-Immunofluorescence-NB400-116-img0004.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/1171) [NBP2-44569] - Human Tonsil stained with CD34 Monoclonal Antibody (HPCA1/1171)](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-1171-Immunohistochemistry-Paraffin-NBP2-44569-img0003.jpg "Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/1171) [NBP2-44569] - Human Tonsil stained with CD34 Monoclonal Antibody (HPCA1/1171)")
![Flow Cytometry: CD34 Antibody (HPCA1/1171) [NBP2-44569] - KG-1 cells using CD34 Monoclonal Antibody (HPCA1/1171) (red) & isotype control (green).](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-1171-Flow-Cytometry-NBP2-44569-img0001.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/1171) [NBP2-44569] - Human Angiosarcoma stained with CD34 Monoclonal Antibody (HPCA1/1171)](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-1171-Immunohistochemistry-Paraffin-NBP2-44569-img0002.jpg)
![Immunohistochemistry-Frozen: Myeloperoxidase/MPO Antibody (8F4) [NBP1-51148] - Frozen 8 um rat liver tissue section fixed in formalin. MPO antibody at 1:50 (green). Multiplexed with a CD68 antibody (red). DAPI staining in blue. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Myeloperoxidase-MPO-Antibody-8F4-Immunohistochemistry-Frozen-NBP1-51148-img0001.jpg "Immunohistochemistry-Frozen: Myeloperoxidase/MPO Antibody (8F4) [NBP1-51148] - Frozen 8 um rat liver tissue section fixed in formalin. MPO antibody at 1:50 (green). Multiplexed with a CD68 antibody (red). DAPI staining in blue. IHC-Fr image submitted by a verified customer review.")
![Western Blot: CCR4 Antibody (HL2481) - Azide and BSA Free [NBP3-25402] - Non-transfected (-) and transfected (+) bolied and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25402_rabbit-ccr4-mab-hl2481-azide-and-bsa-free-2120249425444.jpg "Western Blot: CCR4 Antibody (HL2481) - Azide and BSA Free [NBP3-25402] - Non-transfected (-) and transfected (+) bolied and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: CCR4 Antibody (HL2481) - Azide and BSA Free [NBP3-25402] - CCR4 antibody [HL2481] detects CCR4 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded rat thymus gland. CCR4 stained by CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25402_rabbit-ccr4-mab-hl2481-azide-and-bsa-free-2120249351349.jpg)
![Western Blot: CCR4 Antibody (HL2481) - Azide and BSA Free [NBP3-25402] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25402_rabbit-ccr4-mab-hl2481-azide-and-bsa-free-2120249413373.jpg)
![Western Blot: CCR4 Antibody (HL2481) - Azide and BSA Free [NBP3-25402] - Non-transfected (-) and transfected (+) boiled and unboiled SW480 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25402_rabbit-ccr4-mab-hl2481-azide-and-bsa-free-2120249351389.jpg)
![Western Blot: CCR4 Antibody (HL2481) - Azide and BSA Free [NBP3-25402] - Unboiled SW480 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. (WCE: whole cell extract; ME: membrane extract)](http://images.novusbio.com/fullsize/nbp3-25402_rabbit-ccr4-mab-hl2481-azide-and-bsa-free-2120249355539.jpg)
![Immunohistochemistry-Paraffin: CCR4 Antibody (HL2481) - Azide and BSA Free [NBP3-25402] - CCR4 antibody [HL2481] detects CCR4 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse thymus gland. CCR4 stained by CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25402_rabbit-ccr4-mab-hl2481-azide-and-bsa-free-2120249371563.jpg)
![<b>Genetic Strategies Validation. </b>CCR4 antibody [HL2481] detects CCR4 protein by immunofluorescent analysis.</br>Sample: SW480 cells were fixed in ice-cold MeOH for 5 min.</br>Green: CCR4 stained by CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:200.</br>Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25402_rabbit-ccr4-mab-hl2481-azide-and-bsa-free-immunocytochemistry-immunofluorescence-2611202518342622.jpg)
![CCR4 antibody [HL2481] detects CCR4 protein by immunohistochemical analysis.</br>Sample: Paraffin-embedded human tonsil.</br>CCR4 stained by CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:500.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25402_rabbit-ccr4-mab-hl2481-azide-and-bsa-free-immunohistochemistry-paraffin-26112025196213.jpg)
![Unboiled non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25402_rabbit-ccr4-mab-hl2481-azide-and-bsa-free-western-blot-2611202518473213.jpg)
![CCR4 antibody [HL2481] detects CCR4 protein by immunohistochemical analysis.</br>Sample: Paraffin-embedded mouse thymus gland.</br>CCR4 stained by CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:500.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25402_rabbit-ccr4-mab-hl2481-azide-and-bsa-free-immunohistochemistry-paraffin-2611202518331024.jpg)
![Flow Cytometry: CD31/PECAM-1 Antibody (CO.3E-1D4) [NB100-65900] - Staining of sheep peripheral blood platelets with Mouse anti Bovine CD31/PECAM-1 Antibody (CO.3E-1D4).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-CO-3E-1D4-Flow-Cytometry-NB100-65900-img0005.jpg)
![Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (CO.3E-1D4) [NB100-65900] - Bovine Lung stained with CD31 (green) and DAPI counterstain. Image provided courtesy of Hala Nijmeh, PhD and the PI, Evgenia V. Gerasimovskaya, PhD. Section of Critical Care Medicine, Department of Pediatrics, University of Colorado Anschutz Medical Center.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-CO-3E-1D4-Immunohistochemistry-Frozen-NB100-65900-img0006.jpg)
![ELISA: CTLA-4 Antibody (BNI3) [NB100-64849] - <strong>Human Anti-Abatacept Antibody bridging ELISA for Pharmacokinetic (PK) assay development</strong><br><br> A microtiter plate was coated overnight with Mouse Anti-Human CD152 Antibody, clone BNI3 at a concentration of 1 µg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of abatacept. Detection was performed using HRP conjugated Human Anti-Abatacept Antibody, clone AbD37058ia at a concentration of 2 µg/ml in HISPEC Assay Diluent followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit.](http://images.novusbio.com/fullsize/nb100-64849_mouse-monoclonal-ctla-4-antibody-bni3-2312025235988.jpg "ELISA: CTLA-4 Antibody (BNI3) [NB100-64849] - <strong>Human Anti-Abatacept Antibody bridging ELISA for Pharmacokinetic (PK) assay development</strong><br><br> A microtiter plate was coated overnight with Mouse Anti-Human CD152 Antibody, clone BNI3 at a concentration of 1 µg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of abatacept. Detection was performed using HRP conjugated Human Anti-Abatacept Antibody, clone AbD37058ia at a concentration of 2 µg/ml in HISPEC Assay Diluent followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit.")
![Western Blot: IgA Antibody (7D5F12) [NBP2-61794] - Analysis using IghA1 mAb against HEK293 (1) and IghA1 (AA: 207-353)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/IgA-Antibody-7D5F12-Western-Blot-NBP2-61794-img0006.jpg "Western Blot: IgA Antibody (7D5F12) [NBP2-61794] - Analysis using IghA1 mAb against HEK293 (1) and IghA1 (AA: 207-353)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: IgA Antibody (7D5F12) [NBP2-61794] - Analysis of Hela cells using IghA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/IgA-Antibody-7D5F12-Immunocytochemistry-Immunofluorescence-NBP2-61794-img0003.jpg)
![Immunohistochemistry-Paraffin: IgA Antibody (7D5F12) [NBP2-61794] - Analysis of breast cancer tissues using IghA1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/IgA-Antibody-7D5F12-Immunohistochemistry-NBP2-61794-img0004.jpg)
![Flow Cytometry: IgA Antibody (7D5F12) [NBP2-61794] - Analysis of Hela cells using IghA1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/IgA-Antibody-7D5F12-ELISA-NBP2-61794-img0002.jpg)
![Western Blot: IgA Antibody (7D5F12) [NBP2-61794] - Analysis using IghA1 mAb against human IghA1 (AA: 207-353) recombinant protein. (Expected MW is 41.7 kDa)](http://images.novusbio.com/fullsize/IgA-Antibody-7D5F12-Western-Blot-NBP2-61794-img0005.jpg)
![Western Blot: IgA Antibody (7D5F12) [NBP2-61794] - Analysis using IghA1 mouse mAb against L1210 (1), THP-1 (2), HepG2 (3), and COS7 (4) cell lysate.](http://images.novusbio.com/fullsize/IgA-Antibody-7D5F12-Western-Blot-NBP2-61794-img0007.jpg)
![ELISA: IgA Antibody (7D5F12) [NBP2-61794] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/IgA-Antibody-7D5F12-ELISA-NBP2-61794-img0001.jpg)
![Western Blot: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis using HLA-B mAb against HEK293 (1) and HLA-B (AA: 241-362)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/HLA-B-Antibody-2G7B10-Western-Blot-NBP2-61871-img0008.jpg "Western Blot: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis using HLA-B mAb against HEK293 (1) and HLA-B (AA: 241-362)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis of Hela cells using HLA-B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/HLA-B-Antibody-2G7B10-Immunocytochemistry-Immunofluorescence-NBP2-61871-img0004.jpg)
![Immunohistochemistry-Paraffin: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis of bladder cancer tissues using HLA-B mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/HLA-B-Antibody-2G7B10-Immunohistochemistry-NBP2-61871-img0006.jpg)
![Flow Cytometry: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis of MCF-7 cells using HLA-B mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/HLA-B-Antibody-2G7B10-Flow-Cytometry-NBP2-61871-img0002.jpg)
![Western Blot: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis using HLA-B mAb against human HLA-B (AA: 241-362) recombinant protein. (Expected MW is 38.4 kDa)](http://images.novusbio.com/fullsize/HLA-B-Antibody-2G7B10-Western-Blot-NBP2-61871-img0007.jpg)
![Western Blot: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis using HLA-B mouse mAb against Ramos (1) and A431 (2) cell lysate.](http://images.novusbio.com/fullsize/HLA-B-Antibody-2G7B10-Western-Blot-NBP2-61871-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis of Hela cells using HLA-B mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/HLA-B-Antibody-2G7B10-Immunocytochemistry-Immunofluorescence-NBP2-61871-img0003.jpg)
![Immunohistochemistry-Paraffin: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis of ovarian cancer tissues using HLA-B mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/HLA-B-Antibody-2G7B10-Immunohistochemistry-NBP2-61871-img0005.jpg)
![ELISA: HLA B Antibody (2G7B10) [NBP2-61871] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/HLA-B-Antibody-2G7B10-ELISA-NBP2-61871-img0001.jpg)
![Western Blot: BDNF Antibody [NBP1-46750] - Human platelet lysate. Blocking: 1% LFDM for 30 min at RT; primary antibody incubated at 4C overnight. Click on eLAB BOOK tab to see the details. Mature BDNF: (1:2000 dilution) ; proBDNF: (1:1000 dilution)](http://images.novusbio.com/fullsize/BDNF-Antibody-Western-Blot-NBP1-46750-img0002.jpg "Western Blot: BDNF Antibody [NBP1-46750] - Human platelet lysate. Blocking: 1% LFDM for 30 min at RT; primary antibody incubated at 4C overnight. Click on eLAB BOOK tab to see the details. Mature BDNF: (1:2000 dilution) ; proBDNF: (1:1000 dilution)")
![Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750] - IHC-P on paraffin sections of rat hippocampus.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer following manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.Small neurons are stained and also some nuclear staining is observed.](http://images.novusbio.com/fullsize/BDNF-Antibody-Immunohistochemistry-Paraffin-NBP1-46750-img0008.jpg)
![Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750] - Paraffin sections of rat spinal cord. Dilution 1: 1000.](http://images.novusbio.com/fullsize/BDNF-Antibody-Immunohistochemistry-Paraffin-NBP1-46750-img0003.jpg)
![Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750] - Paraffin sections of rat hippocampus. Dilution 1: 1000.](http://images.novusbio.com/fullsize/BDNF-Antibody-Immunohistochemistry-Paraffin-NBP1-46750-img0004.jpg)
![Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750] - Paraffin sections of mouse hippocampus. Dilution 1: 1000.](http://images.novusbio.com/fullsize/BDNF-Antibody-Immunohistochemistry-Paraffin-NBP1-46750-img0005.jpg)
![Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750] - IHC-P on paraffin sections of rat hippocampus.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer following manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.Small neurons are stained and also some nuclear staining is observed.](http://images.novusbio.com/fullsize/BDNF-Antibody-Immunohistochemistry-Paraffin-NBP1-46750-img0007.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of c-Jun [p Ser63] (whole cell lysate, 10 ug /lane. Lane 2: NIH/3T3 cells were treated with 250 ng/ml anisomycin for 30 minutes, whole cell lysates, 10 ug/lane. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody Anti-c-Jun [p Ser63] (1/500) , Anti-c-Jun antibody (1/2000) and Anti-GAPDH antibody (1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 36 kDa Observed band size: 40 kDa Exposure time: 3 minutes 43 seconds](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0010.jpg "<b>Biological Strategies Validation. </b>Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of c-Jun [p Ser63] (whole cell lysate, 10 ug /lane. Lane 2: NIH/3T3 cells were treated with 250 ng/ml anisomycin for 30 minutes, whole cell lysates, 10 ug/lane. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody Anti-c-Jun [p Ser63] (1/500) , Anti-c-Jun antibody (1/2000) and Anti-GAPDH antibody (1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 36 kDa Observed band size: 40 kDa Exposure time: 3 minutes 43 seconds")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunocytochemistry-Immunofluorescence-NBP2-67471-img0006.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human endometrial tissue with Rabbit anti-c-jun [p Ser63] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunohistochemistry-Paraffin-NBP2-67471-img0012.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of Phospho-c-Jun(S63) on different lysates using anti-Phospho-c-Jun(S63) antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: 293T](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0005.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Western blot analysis of c-jun on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0007.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Human U251 glioma cell line, whole cell lysates. Cells have been treated with JNK and p38 inhibitors. WB image submited by a verified customer review.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0008.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of c-Jun (p Ser63) on different lysates with Rabbit anti-c-Jun (p Ser63) antibody at 1/500 dilution. Lane 1: U87MG cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: 293T cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 36 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Western-Blot-NBP2-67471-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunocytochemistry-Immunofluorescence-NBP2-67471-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunocytochemistry-Immunofluorescence-NBP2-67471-img0002.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-c-Jun(S63) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunohistochemistry-Paraffin-NBP2-67471-img0003.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human tonsil tissue using anti-Phospho-c-Jun(S63) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunohistochemistry-Paraffin-NBP2-67471-img0004.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-c-jun [Ser63] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/c-jun-p-Ser63-Antibody-SY0297-Immunohistochemistry-Paraffin-NBP2-67471-img0011.jpg)
![Western blot analysis on different lysates with NBP2-67471 at 1/2,000 dilution. Lane 1: C6 cell lysate Lane 2: C6 treated with 25μg/mL Anisomycin for 30 minutes cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 25μg/mL Anisomycin for 30 minutes cell lysate Lane 5: NIH/3T3 treated with 25μg/mL Anisomycin for 30 minutes, then treated with lambda pp for 1 hour cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 36 kDa Observed band size: 40 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67471 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67471_-western-blot-639204586672256172.jpg)
![Western blot analysis on different lysates with NBP2-67471 at 1/500 dilution. Lane 1: A549 whole cell lysate Lane 2: A549 treated with 250ng/mL anisomycin for 30 minutes whole cell lysate Lane 3: NIH/3T3 whole cell lysate Lane 4: NIH/3T3 treated with 250ng/mL anisomycin for 30 minutes whole cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 36 kDa Observed band size: 40 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67471 at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67471_-western-blot-639204597490896795.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) PE-CyTM7.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0005.jpg "Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) PE-CyTM7.")
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Staining of human peripheral blood with anti-human CD38 (HIT2) PE-CyTM5.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0006.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Analysis (surface staining) of human peripheral blood with anti-human CD38 (HIT2) Pacific Blue(TM).](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0012.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Analysis using the PE conjugate of NB500-510. Surface staining of PHA stimulated human peripheral blood lymphocytes with anti-human CD38 (HIT2) PE.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0002.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) Alexa Fluor(R) 700.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0003.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) FITC.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0004.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) biotin / streptavidin-APC.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0007.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) PerCP.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0008.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) APC-CyTM7.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0009.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) APC.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0010.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) Pacific Orange(TM).](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0011.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (HA111) [NB100-296] - HIF-1 antibody was tested in human ovarian cancer tumor xenograft using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-HA111-Immunohistochemistry-Paraffin-NB100-296-img0001.jpg "Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (HA111) [NB100-296] - HIF-1 antibody was tested in human ovarian cancer tumor xenograft using DAB with hematoxylin counterstain.")
![Western Blot: HIF-1 alpha Antibody (HA111) [NB100-296] - Detection of HIF-1 alpha (125-130 kDa) from human placental villous explant total protein.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-HA111-Western-Blot-NB100-296-img0004.jpg)