| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64852-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97519-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Low Endotoxin, Azide and BSA Free | NB600-1443-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52680-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-18953SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54548-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76798SS | |||
![Immunohistochemistry-Frozen: CD1a Antibody (NA1/34-HLK) [NB100-64852] - Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD1a antibody followed by HISTAR detection system. Medium power](http://images.novusbio.com/fullsize/CD1a-Antibody-NA1-34-HLK-Immunohistochemistry-Frozen-NB100-64852-img0007.jpg "Immunohistochemistry-Frozen: CD1a Antibody (NA1/34-HLK) [NB100-64852] - Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD1a antibody followed by HISTAR detection system. Medium power")
![Flow Cytometry: CD1a Antibody (NA1/34-HLK) [NB100-64852] - Analysis using the FITC conjugate of NB100-64852. Staining of MOLT 4 cells.](http://images.novusbio.com/fullsize/CD1a-Antibody-NA1-34-HLK-Flow-Cytometry-NB100-64852-img0005.jpg)
![Immunohistochemistry-Frozen: CD1a Antibody (NA1/34-HLK) [NB100-64852] - mmunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD1a antibody followed by HISTAR detection system. Low power](http://images.novusbio.com/fullsize/CD1a-Antibody-NA1-34-HLK-Immunohistochemistry-Frozen-NB100-64852-img0006.jpg)
![Flow Cytometry: CD1a Antibody (NA1/34-HLK) [NB100-64852] - Analysis using the Biotin conjugate of NB100-64852. Staining of MOLT 4 cells with CD1a antibody.](http://images.novusbio.com/fullsize/CD1a-Antibody-NA1-34-HLK-Flow-Cytometry-NB100-64852-img0004.jpg)
![Immunohistochemistry-Paraffin: Fas Ligand Antibody (A11) [NBP1-97519] - Immunohistochemical localization of FasL in adult mouse testis using the A11 antibody.](http://images.novusbio.com/fullsize/Fas-Ligand-Antibody-A11-Immunohistochemistry-Paraffin-NBP1-97519-img0001.jpg "Immunohistochemistry-Paraffin: Fas Ligand Antibody (A11) [NBP1-97519] - Immunohistochemical localization of FasL in adult mouse testis using the A11 antibody.")
![Immunohistochemistry: IL-12 Antibody [NB600-1443] - Irradiation-induced IL-6 suppressed IL-12 expression in tumors of hepatoma-bearing mice in vivo and DCs in vitro. IL-6 and IL-12 were measured at 1, 3, and 7 days after 10 Gy irradiation of tumors. Shown are the expressions of IL-6 and IL-12 in HCa-1 tumors (* P < 0.05). BMDCs were prepared from bone marrow of mice and stimulated with LPS. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0146463), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-12-Antibody-Immunohistochemistry-NB600-1443-img0003.jpg "Immunohistochemistry: IL-12 Antibody [NB600-1443] - Irradiation-induced IL-6 suppressed IL-12 expression in tumors of hepatoma-bearing mice in vivo and DCs in vitro. IL-6 and IL-12 were measured at 1, 3, and 7 days after 10 Gy irradiation of tumors. Shown are the expressions of IL-6 and IL-12 in HCa-1 tumors (* P < 0.05). BMDCs were prepared from bone marrow of mice and stimulated with LPS. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0146463), licensed under a CC-BY license.")
![Immunohistochemistry: IL-12 Antibody [NB600-1443] - Irradiation inhibited IL-12 expression in tumors of hepatoma-bearing mice in vivo and DCs in vitro. HCa-1 and MIH-2 cells were injected intramuscularly into the right thighs of the mice, and tumors were irradiated with 10 Gy of radiation. Shown are IL-12 and DC (CD11c+) expressions at 1, 3, and 7 days after irradiation in HCa-1 tumors in the spleens of HCa-1 bearing mice (** P < 0.01). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0146463), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-12-Antibody-Immunohistochemistry-NB600-1443-img0001.jpg)
![Immunohistochemistry: IL-12 Antibody [NB600-1443] - Irradiation inhibited IL-12 expression in tumors of hepatoma-bearing mice in vivo and DCs in vitro. HCa-1 and MIH-2 cells were injected intramuscularly into the right thighs of the mice, and tumors were irradiated with 10 Gy of radiation. Shown are IL-12 and DC (CD11c+) expressions at 1, 3, and 7 days after irradiation in MIH-2 tumors in the spleens of HCa-1 bearing mice (** P < 0.01). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0146463), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-12-Antibody-Immunohistochemistry-NB600-1443-img0002.jpg)
![Immunohistochemistry: IL-12 Antibody [NB600-1443] - Irradiation-induced IL-6 suppressed IL-12 expression in tumors of hepatoma-bearing mice in vivo and DCs in vitro.IL-6 and IL-12 were measured at 1, 3, and 7 days after 10 Gy irradiation of tumors. Shown are the expressions of IL-6 and IL-12 in MIH-2 tumors (* P < 0.05). BMDCs were prepared from bone marrow of mice and stimulated with LPS. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0146463), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-12-Antibody-Immunohistochemistry-NB600-1443-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-12 Antibody [NB600-1443] - Irradiation inhibited IL-12 expression in tumors of hepatoma-bearing mice in vivo & DCs in vitro.HCa-1 & MIH-2 cells were injected intramuscularly into the right thighs of the mice, & tumors were irradiated with 10 Gy of radiation. Shown are IL-12 & DC (CD11c+) expressions at 1, 3, & 7 days after irradiation in (a) HCa-1 & (b) MIH-2 tumors & (c) IL-12 expression in the spleens of HCa-1 bearing mice (** P < 0.01). (d) IL-12 expression in BMDCs incubated with supernatant of MIH-2 tumor cells or supernatant of 10 Gy irradiating MIH-2 tumor cells. (** P < 0.01). DCs were differentiated from bone marrow of C3H/HeN normal mice (BMDCs). These were incubated for 72 h in supernatant of MIH-2 tumor cells with or without 10 Gy irradiation. IL-12 was intracellular stained with IL-12 antibody & analyzed by FACS. BMDCs also were stimulated with LPS (100 ng/mL) to allow maturation & irradiated with 10 Gy of radiation. (e) IL-12 expression (** P < 0.01), (f) cell viability, & (g) B7.1 expression in LPS stimulated BMDCs receiving 10 Gy radiation. (* P < 0.05). Data are from three independent experiments with five mice per group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26745884), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-1443_goat-polyclonal-il-12-antibody-31020241616187.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-12 Antibody [NB600-1443] - Irradiation-induced IL-6 suppressed IL-12 expression in tumors of hepatoma-bearing mice in vivo & DCs in vitro.IL-6 & IL-12 were measured at 1, 3, & 7 days after 10 Gy irradiation of tumors. Shown are the expressions of IL-6 & IL-12 in (a) HCa-1 & (b) MIH-2 tumors (* P < 0.05). BMDCs were prepared from bone marrow of mice & stimulated with LPS. (c) IL-6 expression analyzed at the indicated times by ELISA in irradiated or non-irradiated BMDCs in vitro. (d) IL-6R (** P < 0.01, vs No IR) & (e) sIL6R (** P < 0.01) were evaluated at 2 day after 10 Gy irradiation to BMDCs by FACS & ELISA, respectively. BMDCs were treated without or with IL-6 (20 ng/mL) plus LPS. (f) Down regulation of IL-12 production by IL-6 treatment in BMDCs (** P < 0.01). BMDCs were stimulated with LPS for activation & cultured with or without anti-IL-6 antibody (final concentration of 500 ng/mL) during the indicated times after irradiation in vitro. IL-6 & IL-12 were analyzed by ELISA. (g) Recovery of IL-12 production & (h) B7-1/ B7-2 expression using an anti-IL-6 antibody in irradiated-BMDCs stimulated with LPS in vitro. (** P < 0.01, * P < 0.05). Data are from three independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26745884), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-1443_goat-polyclonal-il-12-antibody-310202416182614.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-12 Antibody [NB600-1443] - Irradiation-induced IL-6 suppressed IL-12 expression in tumors of hepatoma-bearing mice in vivo & DCs in vitro.IL-6 & IL-12 were measured at 1, 3, & 7 days after 10 Gy irradiation of tumors. Shown are the expressions of IL-6 & IL-12 in (a) HCa-1 & (b) MIH-2 tumors (* P < 0.05). BMDCs were prepared from bone marrow of mice & stimulated with LPS. (c) IL-6 expression analyzed at the indicated times by ELISA in irradiated or non-irradiated BMDCs in vitro. (d) IL-6R (** P < 0.01, vs No IR) & (e) sIL6R (** P < 0.01) were evaluated at 2 day after 10 Gy irradiation to BMDCs by FACS & ELISA, respectively. BMDCs were treated without or with IL-6 (20 ng/mL) plus LPS. (f) Down regulation of IL-12 production by IL-6 treatment in BMDCs (** P < 0.01). BMDCs were stimulated with LPS for activation & cultured with or without anti-IL-6 antibody (final concentration of 500 ng/mL) during the indicated times after irradiation in vitro. IL-6 & IL-12 were analyzed by ELISA. (g) Recovery of IL-12 production & (h) B7-1/ B7-2 expression using an anti-IL-6 antibody in irradiated-BMDCs stimulated with LPS in vitro. (** P < 0.01, * P < 0.05). Data are from three independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26745884), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-1443_goat-polyclonal-il-12-antibody-310202416154619.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-12 Antibody [NB600-1443] - Irradiation inhibited IL-12 expression in tumors of hepatoma-bearing mice in vivo & DCs in vitro.HCa-1 & MIH-2 cells were injected intramuscularly into the right thighs of the mice, & tumors were irradiated with 10 Gy of radiation. Shown are IL-12 & DC (CD11c+) expressions at 1, 3, & 7 days after irradiation in (a) HCa-1 & (b) MIH-2 tumors & (c) IL-12 expression in the spleens of HCa-1 bearing mice (** P < 0.01). (d) IL-12 expression in BMDCs incubated with supernatant of MIH-2 tumor cells or supernatant of 10 Gy irradiating MIH-2 tumor cells. (** P < 0.01). DCs were differentiated from bone marrow of C3H/HeN normal mice (BMDCs). These were incubated for 72 h in supernatant of MIH-2 tumor cells with or without 10 Gy irradiation. IL-12 was intracellular stained with IL-12 antibody & analyzed by FACS. BMDCs also were stimulated with LPS (100 ng/mL) to allow maturation & irradiated with 10 Gy of radiation. (e) IL-12 expression (** P < 0.01), (f) cell viability, & (g) B7.1 expression in LPS stimulated BMDCs receiving 10 Gy radiation. (* P < 0.05). Data are from three independent experiments with five mice per group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26745884), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-1443_goat-polyclonal-il-12-antibody-310202416163756.jpg)
![Western Blot: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Analysis of THY1 on different lysates using anti-THY1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: HUVEC](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Western-Blot-NBP2-66763-img0007.jpg "Western Blot: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Analysis of THY1 on different lysates using anti-THY1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: HUVEC")
![Immunocytochemistry/Immunofluorescence: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Staining THY1 in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunocytochemistry-Immunofluorescence-NBP2-66763-img0008.jpg)
![Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Analysis of paraffin-embedded mouse hippocampus tissue using anti-CD90/Thy1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunohistochemistry-Paraffin-NBP2-66763-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Staining THY1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunocytochemistry-Immunofluorescence-NBP2-66763-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Staining THY1 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunocytochemistry-Immunofluorescence-NBP2-66763-img0002.jpg)
![Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Analysis of paraffin-embedded human kidney tissue using anti-THY1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunohistochemistry-Paraffin-NBP2-66763-img0003.jpg)
![Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Analysis of paraffin-embedded human lung cancer tissue using anti-THY1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunohistochemistry-Paraffin-NBP2-66763-img0004.jpg)
![Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Analysis of paraffin-embedded human spleen tissue using anti-THY1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunohistochemistry-Paraffin-NBP2-66763-img0005.jpg)
![Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Analysis of paraffin-embedded human tonsil tissue using anti-THY1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunohistochemistry-Paraffin-NBP2-66763-img0006.jpg)
![Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (JF10-09) [NBP2-66763] - Analysis of paraffin-embedded mouse brain tissue using anti-CD90/Thy1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-JF10-09-Immunohistochemistry-Paraffin-NBP2-66763-img0009.jpg)
![Western Blot: CCR7 Antibody (SR36-04) [NBP2-67324] - Analysis of CCR7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysateLane 2: MCF-7 cell lysate Lane 3: PC-12 cell lysate Lane 4: Daudi cell lysate](http://images.novusbio.com/fullsize/CCR7-Antibody-SR36-04-Western-Blot-NBP2-67324-img0009.jpg "Western Blot: CCR7 Antibody (SR36-04) [NBP2-67324] - Analysis of CCR7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysateLane 2: MCF-7 cell lysate Lane 3: PC-12 cell lysate Lane 4: Daudi cell lysate")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CCR7 Antibody (SR36-04) [NBP2-67324] - Gross and histological appearance of lung excised from 13 weeks of age PyMT transgenic females. CRE treated PyMT transgenic females showed decreased frequency of mammary tumor-derived lung metastases. Immunohistochemistry staining against Ccr7 in lung tissues was done and representative images of tissue sections from each group were taken at 200X and 40X magnification. Scale bars = 50um. Histogram data was presented as mean +/- SEM. * p < 0.05 vs. control. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6643/11/2/410), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCR7-Antibody-SR36-04-Immunocytochemistry-Immunofluorescence-NBP2-67324-img0006.jpg)
![Immunohistochemistry-Paraffin: CCR7 Antibody (SR36-04) [NBP2-67324] - Analysis of paraffin-embedded human tonsil tissue using anti-CCR7 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CCR7-Antibody-SR36-04-Immunohistochemistry-Paraffin-NBP2-67324-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: CCR7 Antibody (SR36-04) [NBP2-67324] - Gross and histological appearance of lung excised from 13 weeks of age PyMT transgenic females. CRE treated PyMT transgenic females showed decreased frequency of mammary tumor-derived lung metastases. Level of Ccr7 protein in lung tissues was analyzed by Western blot. Gapdh was used as an internal control. * p < 0.05 vs. control. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6643/11/2/410), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCR7-Antibody-SR36-04-Western-Blot-NBP2-67324-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CCR7 Antibody (SR36-04) [NBP2-67324] - Staining CCR7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CCR7-Antibody-SR36-04-Immunocytochemistry-Immunofluorescence-NBP2-67324-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CCR7 Antibody (SR36-04) [NBP2-67324] - Staining CCR7 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CCR7-Antibody-SR36-04-Immunocytochemistry-Immunofluorescence-NBP2-67324-img0002.jpg)
![Immunohistochemistry-Paraffin: CCR7 Antibody (SR36-04) [NBP2-67324] - Analysis of paraffin-embedded human spleen tissue using anti-CCR7 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CCR7-Antibody-SR36-04-Immunohistochemistry-Paraffin-NBP2-67324-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CCR7 Antibody (SR36-04) [NBP2-67324] - Downregulation of CCR7 gene by siRNA inhibits gene expression of MMP9, c-FOS, c-JUN and CCL21 in vitro. Western blot of proteins extracted from MCF7 cell against CCR7 and GAPDH. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6643/11/2/410), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCR7-Antibody-SR36-04-Knockdown-Validated-NBP2-67324-img0007.jpg)
![Western Blot: CCR7 Antibody (SR36-04) [NBP2-67324] - Curcumae radix extract treatment exhibited anti-metastatic effects on primary tumor of MMTV-PyMT transgenic mouse. (A) Quantitative analysis of real-time PCR was done using genes associated with breast cancer metastasis. The expression levels of Ccr7, c-Fos, Mmp9, Ccl21, & Ccl19 were determined by qRT-PCR. The Hprt gene was used as input control, respectively, * p < 0.05 & ** p < 0.005 vs. control. (B) Western blot of proteins extracted from tumor tissues in the PyMT transgenic model against Ccr7 & Gapdh. Histogram data was presented as mean ± SEM. * p < 0.05 vs. control. (C) Western blot of proteins extracted from tumor tissues against Phosphorylated Akt, Phosphorylated Ampk, Her2, ERalpha, Gapdh & beta -actin. (D) Quantitative analysis of real time PCR was done by using genes associated with apoptosis. The expression levels of Jun, Akt, & Tnf were determined by qRT-PCR. Data are representative of three independent experiments & are expressed as the mean ± SD. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30781353), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-67324_rabbit-monoclonal-ccr7-antibody-sr36-04-3102024161224.jpg)
![Western Blot: IRF3 Antibody (SD2062) [NBP2-67741] - Analysis of IRF3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysateLane 2: Hela cell lysate](http://images.novusbio.com/fullsize/IRF3-Antibody-SD2062-Western-Blot-NBP2-67741-img0013.jpg "Western Blot: IRF3 Antibody (SD2062) [NBP2-67741] - Analysis of IRF3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysateLane 2: Hela cell lysate")
![Immunocytochemistry/Immunofluorescence: IRF3 Antibody (SD2062) [NBP2-67741] - Staining IRF3 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/IRF3-Antibody-SD2062-Immunocytochemistry-Immunofluorescence-NBP2-67741-img0010.jpg)
![Immunohistochemistry-Paraffin: IRF3 Antibody (SD2062) [NBP2-67741] - Analysis of paraffin-embedded mouse pancreas tissue using anti-IRF3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/IRF3-Antibody-SD2062-Immunohistochemistry-Paraffin-NBP2-67741-img0011.jpg)
![Flow Cytometry: IRF3 Antibody (SD2062) [NBP2-67741] - Flow cytometric analysis of IRF3 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/IRF3-Antibody-SD2062-Flow-Cytometry-NBP2-67741-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: IRF3 Antibody (SD2062) [NBP2-67741] - Staining IRF3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/IRF3-Antibody-SD2062-Immunocytochemistry-Immunofluorescence-NBP2-67741-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: IRF3 Antibody (SD2062) [NBP2-67741] - Staining IRF3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/IRF3-Antibody-SD2062-Immunocytochemistry-Immunofluorescence-NBP2-67741-img0002.jpg)
![Immunohistochemistry-Paraffin: IRF3 Antibody (SD2062) [NBP2-67741] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-IRF3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/IRF3-Antibody-SD2062-Immunohistochemistry-Paraffin-NBP2-67741-img0003.jpg)
![Immunohistochemistry-Paraffin: IRF3 Antibody (SD2062) [NBP2-67741] - Analysis of paraffin-embedded human kidney tissue using anti-IRF3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/IRF3-Antibody-SD2062-Immunohistochemistry-Paraffin-NBP2-67741-img0004.jpg)
![Immunohistochemistry-Paraffin: IRF3 Antibody (SD2062) [NBP2-67741] - Analysis of paraffin-embedded human pancreas tissue using anti-IRF3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/IRF3-Antibody-SD2062-Immunohistochemistry-Paraffin-NBP2-67741-img0005.jpg)
![Immunohistochemistry-Paraffin: IRF3 Antibody (SD2062) [NBP2-67741] - Analysis of paraffin-embedded human tonsil tissue using anti-IRF3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/IRF3-Antibody-SD2062-Immunohistochemistry-Paraffin-NBP2-67741-img0006.jpg)
![Immunohistochemistry-Paraffin: IRF3 Antibody (SD2062) [NBP2-67741] - Analysis of paraffin-embedded mouse kidney tissue using anti-IRF3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/IRF3-Antibody-SD2062-Immunohistochemistry-Paraffin-NBP2-67741-img0007.jpg)
![Immunohistochemistry-Paraffin: IRF3 Antibody (SD2062) [NBP2-67741] - Analysis of paraffin-embedded mouse spleen tissue using anti-IRF3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/IRF3-Antibody-SD2062-Immunohistochemistry-Paraffin-NBP2-67741-img0008.jpg)
![Flow Cytometry: IRF3 Antibody (SD2062) [NBP2-67741] - Analysis of Hela cells with IRF3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/IRF3-Antibody-SD2062-Flow-Cytometry-NBP2-67741-img0012.jpg)
![Western Blot: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of NCAM-1/CD56 on human brain tissue lysates with Rabbit anti-NCAM/CD56 antibody at 1/2,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 95 kDa Observed band size: 140/180 kDa Exposure time: 30 seconds; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Western-Blot-NBP2-66968-img0009.jpg "Western Blot: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of NCAM-1/CD56 on human brain tissue lysates with Rabbit anti-NCAM/CD56 antibody at 1/2,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 95 kDa Observed band size: 140/180 kDa Exposure time: 30 seconds; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Staining NCAM in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunocytochemistry-Immunofluorescence-NBP2-66968-img0007.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-NCAM-1/CD56 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunohistochemistry-Paraffin-NBP2-66968-img0011.jpg)
![Flow Cytometry: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of SH-SY-5Y cells with NCAM antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Flow-Cytometry-NBP2-66968-img0001.jpg)
![Western Blot: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of NCAM on different lysates using anti-NCAM antibody at 1/1,000 dilution. Positive control: Lane 1: SH-SY-5Y Lane 2: Human brain](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Western-Blot-NBP2-66968-img0006.jpg)
![Western Blot: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of NCAM-1/CD56 on SH-SY5Y cell lysates with Rabbit anti-NCAM-1/CD56 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 95 kDa Observed band size: 120/140 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Western-Blot-NBP2-66968-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Staining NCAM in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunocytochemistry-Immunofluorescence-NBP2-66968-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Staining NCAM in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunocytochemistry-Immunofluorescence-NBP2-66968-img0003.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of paraffin-embedded human tonsil tissue using anti-NCAM antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunohistochemistry-Paraffin-NBP2-66968-img0004.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of paraffin-embedded zebrafish kidney tissue using anti-NCAM antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunohistochemistry-Paraffin-NBP2-66968-img0005.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of paraffin-embedded human tonsil tissue using anti-NCAM-1/CD56 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunohistochemistry-Paraffin-NBP2-66968-img0010.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - N87 human gastric tumor xenograft immunostained with isotope control antibody (left panel) or 1:200 dilution of Integrin alpha 5 beta 1 antibody (right panel). Arrows indicate DAB immunoreactivity. Red scale bar = 10 microns. Image from verified customer review.](http://images.novusbio.com/fullsize/Integrin-alpha-5-beta-1-Antibody-M200-Volociximab-Chimeric-Immunohistochemistry-Paraffin-NBP2-52680-img0003.jpg "Immunohistochemistry-Paraffin: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - N87 human gastric tumor xenograft immunostained with isotope control antibody (left panel) or 1:200 dilution of Integrin alpha 5 beta 1 antibody (right panel). Arrows indicate DAB immunoreactivity. Red scale bar = 10 microns. Image from verified customer review.")
![Flow Cytometry: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - Flow-cytometry using the anti-alpha 5 beta 1 Integrin M200 (Volociximab). PBMC's (A) and U93 cells (B) were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of M200 (Volociximab) (NBP2-52680, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Integrin-alpha-5-beta-1-Antibody-M200-Volociximab-Chimeric-Flow-Cytometry-NBP2-52680-img0002.jpg)
![Immunofluorescence: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - Immunofluoresence staining of fixed HeLa cells with anti-alpha 5 beta 1 Integrin antibody M200 (Volociximab). Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of M200 (NBP2-52680) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-52680, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Integrin-alpha-5-beta-1-Antibody-M200-Volociximab-Chimeric-Immunofluorescence-NBP2-52680-img0001.jpg)
![Flow Cytometry: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric - Azide and BSA Free [NBP2-52680] - Abundance of two potential Hla receptors & of caveolin-1 as a potential pore-stabilizing factor.A: Representative examples of immune fluorescence assays using epifluorescence microscopy (nuclei counterstained using DAPI) that were performed on 16HBE14o-, S9 or A549 cells grown on coverslips using antibodies against ADAM10, the alpha 5 beta 1 integrin or against caveolin-1. Staining appearing in red represents specific labelling of the respective proteins. Scale bars: 10 μm. B: Semi-quantitative determination of primary & secondary antibody-mediated fluorescence in suspended individual cells by flow cytometry. During flow cytometry, the fluorescence of the antibody-tagged proteins per cell was measured & the respective medians of the detected peaks were used for calculating the means ± S.D. for the biological replicates (n = 4, each). Individual means were tested for significant differences using Student’s t-test or Welch’s t-test: * = p ≤ 0.05, ** = p ≤ 0.01 or *** = p ≤ 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32470006), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-52680_rabbit-monoclonal-integrin-alpha-5-beta-1-antibody-m200-volociximab-chimeric-310202416114751.jpg)
![Immunocytochemistry/Immunofluorescence: HPd1 Antibody (DHIC2-4A10) [NBP1-18953] - Immunofluorescence on frozen section of human pancreas.](http://images.novusbio.com/fullsize/HPd1-Antibody-DHIC2-4A10-Immunocytochemistry-Immunofluorescence-NBP1-18953-img0005.jpg "Immunocytochemistry/Immunofluorescence: HPd1 Antibody (DHIC2-4A10) [NBP1-18953] - Immunofluorescence on frozen section of human pancreas.")
![Flow Cytometry: HPd1 Antibody (DHIC2-4A10) [NBP1-18953] - Analysis of enzyme dispersed human pancreas cells.](http://images.novusbio.com/fullsize/HPd1-Antibody-DHIC2-4A10-Flow-Cytometry-NBP1-18953-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: CD81 Antibody (1.3.3.22) [NBP2-53318] - CD81 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line but is not detected in CD81 knockout (KO) HeLa cell line using Mouse Anti-Human CD81 Monoclonal Antibody (Catalog # NBP2-53318) at 1 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane.](http://images.novusbio.com/fullsize/CD81-Antibody-1-3-3-22-Knockout-Validated-NBP2-53318-img0005.jpg "<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: CD81 Antibody (1.3.3.22) [NBP2-53318] - CD81 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line but is not detected in CD81 knockout (KO) HeLa cell line using Mouse Anti-Human CD81 Monoclonal Antibody (Catalog # NBP2-53318) at 1 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: CD81 Antibody (1.3.3.22) [NBP2-53318] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD81 Mouse Monoclonal Antibody (1.3.3.22).](http://images.novusbio.com/fullsize/CD81-Antibody-1-3-3-22-Immunohistochemistry-Paraffin-NBP2-53318-img0002.jpg)
![Flow Cytometry: CD81 Antibody (1.3.3.22) [NBP2-53318] - Flow cytometry analysis of bead-bound exosomes derived from MCF-7 cells. Unstained exosomes (gray) or exosomes stained with CF568-labeled CD81 monoclonal antibody (1.3.3.22) (orange).](http://images.novusbio.com/fullsize/CD81-Antibody-1-3-3-22-Flow-Cytometry-NBP2-53318-img0007.jpg)
![Immunohistochemistry-Paraffin: CD81 Antibody (1.3.3.22) [NBP2-53318] - Formalin-fixed, paraffin-embedded human Lymph Node stained with CD81 Mouse Monoclonal Antibody (1.3.3.22).](http://images.novusbio.com/fullsize/CD81-Antibody-1-3-3-22-Immunohistochemistry-Paraffin-NBP2-53318-img0001.jpg)
![Flow Cytometry: CD81 Antibody (1.3.3.22) [NBP2-53318] - Flow cytometry analysis of MCF-7 cells unstained (gray) or stained with CD81 mouse monoclonal antibody (1.3.3.22) and goat anti-mouse CF647 (red).](http://images.novusbio.com/fullsize/CD81-Antibody-1-3-3-22-Flow-Cytometry-NBP2-53318-img0006.jpg)
![Western Blot: Stathmin 1 Antibody [NBP1-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Stathmin 1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human SIRT1 Polyclonal Antibody (Catalog # NBP1-76798) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Stathmin 1 at approximately 17 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Knockout-Validated-NBP1-76798-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Stathmin 1 Antibody [NBP1-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Stathmin 1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human SIRT1 Polyclonal Antibody (Catalog # NBP1-76798) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Stathmin 1 at approximately 17 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Stathmin 1 Antibody [NBP1-76798] - EL4 cell lysate with op18 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Western-Blot-NBP1-76798-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Stathmin 1 Antibody [NBP1-76798] - Stathmin 1 antibody was tested in PC12 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76798-img0004.jpg)
![Immunohistochemistry-Paraffin: Stathmin 1 Antibody [NBP1-76798] - Human brain tissue with op18 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-76798-img0002.jpg)