| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50163-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81088-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11404 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54355-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24701SS | |||
![Flow Cytometry: CHD4 Antibody (CHD4 3F2/4) [NBP2-50163] - An intracellular stain was performed on HeLa cells with CHD4 Antibody (CHD4 3F2/4) NBP2-50163PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/CHD4-Antibody-CHD4-3F2-4-Flow-Cytometry-NBP2-50163-img0001.jpg "Flow Cytometry: CHD4 Antibody (CHD4 3F2/4) [NBP2-50163] - An intracellular stain was performed on HeLa cells with CHD4 Antibody (CHD4 3F2/4) NBP2-50163PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.")
![Flow Cytometry: CD44 Antibody (Hermes-1) - Chimeric [NBP2-81088] - Flow-cytometry using anti-CD44 antibody (Hermes-1) [NBP2-81088]. Human activated T lymphocytes were stained with an isotype control (NBP2-52638, panel A) or the rabbit-chimeric version of Hermes-1 (NBP2-81088, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD44-Antibody-Hermes-1-Chimeric-Flow-Cytometry-NBP2-81088-img0001.jpg "Flow Cytometry: CD44 Antibody (Hermes-1) - Chimeric [NBP2-81088] - Flow-cytometry using anti-CD44 antibody (Hermes-1) [NBP2-81088]. Human activated T lymphocytes were stained with an isotype control (NBP2-52638, panel A) or the rabbit-chimeric version of Hermes-1 (NBP2-81088, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
![Western Blot: beta 2-Microglobulin Antibody (C21.48A1) [NBP2-44470] - Western Blot Analysis of THP-1 and Raji cell lysate using beta 2-Microglobulin Antibody (C21.48A1).](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-C21-48A1-Western-Blot-NBP2-44470-img0005.jpg "Western Blot: beta 2-Microglobulin Antibody (C21.48A1) [NBP2-44470] - Western Blot Analysis of THP-1 and Raji cell lysate using beta 2-Microglobulin Antibody (C21.48A1).")
![Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody (C21.48A1) [NBP2-44470] - Immunofluorescent staining of HeLa cells. beta 2-Microglobulin Mouse Monoclonal Antibody (C21.48A1) followed by goat anti-Mouse IgG-CF488 (Green).](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-C21-48A1-Immunocytochemistry-Immunofluorescence-NBP2-44470-img0004.jpg)
![Flow Cytometry: beta 2-Microglobulin Antibody (C21.48A1) [NBP2-44470] - Flow Cytometric Analysis of PFA-fixed HeLa cells using beta 2-Microglobulin Antibody (C21.48A1)followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-C21-48A1-Flow-Cytometry-NBP2-44470-img0002.jpg)
![Western Blot: CHD4 Antibody [NB100-57519] - Whole cell lysate (50 ug) from NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHD4/Mi2 beta antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CHD4-Antibody-Western-Blot-NB100-57519-img0005.jpg "Western Blot: CHD4 Antibody [NB100-57519] - Whole cell lysate (50 ug) from NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHD4/Mi2 beta antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: CHD4 Antibody [NB100-57519] - Detection of Human CHD4/Mi2 beta by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHD4/Mi2 beta antibody NB100-57519 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CHD4-Antibody-Western-Blot-NB100-57519-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CHD4 Antibody [NB100-57519] - Detection of human CHD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CHD4 antibody NB100-57519 used for IP at 6 ug per reaction. CHD4 was also immunoprecipitated by rabbit anti-CHD4 antibodies (NB100-57520, NB100-57521), and rabbit anti-CHD4 recombinant monoclonal antibody [BLR066G] (NBP2-77358). For blotting immunoprecipitated CHD4, NBP2-77358 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/CHD4-Antibody-Immunoprecipitation-NB100-57519-img0006.jpg)
![Immunohistochemistry-Paraffin: pIgR Antibody [NBP1-32897] - Paraffin-embedded human lung adenocarcinoma. PIGR antibody [N2C1], Internal diluted at 1:500.](http://images.novusbio.com/fullsize/pIgR-Antibody-Immunohistochemistry-Paraffin-NBP1-32897-img0005.jpg "Immunohistochemistry-Paraffin: pIgR Antibody [NBP1-32897] - Paraffin-embedded human lung adenocarcinoma. PIGR antibody [N2C1], Internal diluted at 1:500.")
![Immunohistochemistry-Paraffin: pIgR Antibody [NBP1-32897] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/pIgR-Antibody-Immunohistochemistry-Paraffin-NBP1-32897-img0007.jpg)
![HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PIGR stained by PIGR antibody [N2C1], Internal diluted at 1:500.Blue: Fluoroshield with DAPI. Scale bar= 10 μm.](http://images.novusbio.com/fullsize/antibody/nbp1-32897_rabbit-polyclonal-pigr-antibody-immunocytochemistry-immunofluorescence-273202392020.jpg)
![Human milk (30 ug) was separated by 7.5% SDS-PAGE, and the membranes were blotted with PIGR antibody [N2C1], Internal (NBP1-32897) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody](http://images.novusbio.com/fullsize/nbp1-32897_rabbit-polyclonal-pigr-antibody-western-blot-261120251849132.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP2-19654] - Human breast cancer, using P2X7 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP2-19654-img0002.jpg "Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP2-19654] - Human breast cancer, using P2X7 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: CTGF/CCN2 Antibody [NBP2-16026] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-Western-Blot-NBP2-16026-img0007.jpg "Western Blot: CTGF/CCN2 Antibody [NBP2-16026] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: CTGF Antibody [NBP2-16026] - Sample (30 ug of whole cell lysate) A: HUVEC 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/CTGF-Antibody-Western-Blot-NBP2-16026-img0001.jpg)
![Western Blot: Alpha Actinin 3 Antibody [NBP2-15356] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 3 antibody diluted by 1:10000.](http://images.novusbio.com/fullsize/Alpha-Actinin-3-Antibody-Western-Blot-NBP2-15356-img0005.jpg "Western Blot: Alpha Actinin 3 Antibody [NBP2-15356] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 3 antibody diluted by 1:10000.")
![Western Blot: Alpha Actinin 3 Antibody [NBP2-15356] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7. 5% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/Alpha-Actinin-3-Antibody-Western-Blot-NBP2-15356-img0001.jpg)
![Western Blot: Alpha Actinin 3 Antibody [NBP2-15356] - Sample (30 ug of whole cell lysate) A: PC-12 7. 5% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/Alpha-Actinin-3-Antibody-Western-Blot-NBP2-15356-img0002.jpg)
![Immunoprecipitation: Alpha Actinin 3 Antibody [NBP2-15356] - Immunoprecipitation of alpha Actinin 3 protein from HeLa whole cell extracts using 5 ug of alpha Actinin 3 antibody. Western blot analysis was performed using alpha Actinin 3 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Alpha-Actinin-3-Antibody-Immunoprecipitation-NBP2-15356-img0004.jpg)
![Western Blot: Alpha Actinin 3 Antibody [NBP2-15356] - Sample (30 ug of whole cell lysate) A: whole zebrafish 7.5% SDS PAGE NBP2-15356 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp2-15356_rabbit-polyclonal-alpha-actinin-3-antibody-9102024205628.jpg)
![Western Blot: CHD4 Antibody [NB100-57521] - Whole cell lysate from HeLa (5, 15 and 50 ug, HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CHD4 antibody used at 0.04 ug/ml. Detection: Chemiluminescence with exposure times of 3seconds.](http://images.novusbio.com/fullsize/CHD4-Antibody-Western-Blot-NB100-57521-img0004.jpg "Western Blot: CHD4 Antibody [NB100-57521] - Whole cell lysate from HeLa (5, 15 and 50 ug, HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CHD4 antibody used at 0.04 ug/ml. Detection: Chemiluminescence with exposure times of 3seconds.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CHD4 Antibody [NB100-57521] - Detection of human CHD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CHD4 antibody NB100-57521 used for IP at 6 ug per reaction. CHD4 was also immunoprecipitated by rabbit anti-CHD4 antibodies (NB100-57519, NB100-57520), and rabbit anti-CHD4 recombinant monoclonal antibody [BLR066G] (NBP2-77358). Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/CHD4-Antibody-NB100-57521-img0003.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/1806R) [NBP2-53353] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD34 Antibody (HPCA1/1806R).](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-1806R-Immunohistochemistry-Paraffin-NBP2-53353-img0002.jpg "Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/1806R) [NBP2-53353] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD34 Antibody (HPCA1/1806R).")
![Flow Cytometry: CD34 Antibody (HPCA1/1806R) [NBP2-53353] - Flow Cytometric Analysis of human Jurkat cells using CD34 Antibody (HPCA1/1806R) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-1806R-Flow-Cytometry-NBP2-53353-img0003.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/1806R) [NBP2-53353] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/1806R)](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-1806R-Immunohistochemistry-Paraffin-NBP2-53353-img0001.jpg)
![Western Blot: Dkk-1 Antibody [NBP2-24701] - Analysis of human Dkk-1 in A549 cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) RAW using NBP2-24701 at 1 ug/ml.](http://images.novusbio.com/fullsize/Dkk-1-Antibody-Western-Blot-NBP2-24701-img0003.jpg "Western Blot: Dkk-1 Antibody [NBP2-24701] - Analysis of human Dkk-1 in A549 cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) RAW using NBP2-24701 at 1 ug/ml.")
![Western Blot: IL-6 Antibody [NBP1-77894] - Shows detection of recombinant mouse IL-6 raised in E.coli. Recombinant truncated protein (0.1 ug, 21.7 kDa) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-Mouse IL-6 antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4C. After washes, the blot was reacted with secondary antibody DyLight (TM) 649 conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody diluted 1:20,000 in blocking buffer for 30 min. at RT.](http://images.novusbio.com/fullsize/IL-6-Antibody-Western-Blot-NBP1-77894-img0002.jpg "Western Blot: IL-6 Antibody [NBP1-77894] - Shows detection of recombinant mouse IL-6 raised in E.coli. Recombinant truncated protein (0.1 ug, 21.7 kDa) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-Mouse IL-6 antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4C. After washes, the blot was reacted with secondary antibody DyLight (TM) 649 conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody diluted 1:20,000 in blocking buffer for 30 min. at RT.")
![Immunohistochemistry: IL-6 Antibody [NBP1-77894] - Shows nuclear positivity of islets of Langerhans (brown staining) and cytoplasmic staining in mouse pancreas at 10x and 20x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval with E1 (Leica Microsystems) retrieval solution for 20 min and then incubated with rabbit anti-mouse IL-6 antibody at 1:50 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3’3-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.](http://images.novusbio.com/fullsize/IL-6-Antibody-Immunohistochemistry-NBP1-77894-img0004.jpg)
