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![Western Blot: Calreticulin Antibody (FMC 75) [NBP1-97502] - Analysis of Calreticulin: Lane 1: HeLa (Heat Shocked), Lane 2: Vero.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-FMC-75-Western-Blot-NBP1-97502-img0002.jpg "Western Blot: Calreticulin Antibody (FMC 75) [NBP1-97502] - Analysis of Calreticulin: Lane 1: HeLa (Heat Shocked), Lane 2: Vero.")
![Immunohistochemistry-Paraffin: Calreticulin Antibody (FMC 75) [NBP1-97502] - Analysis of human uterus tissue stained with Calreticulin, mAb (FMC 75) at 10ug/ml.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-FMC-75-Immunohistochemistry-Paraffin-NBP1-97502-img0001.jpg)
![Western Blot: RP105/CD180 Antibody [NBP2-20204] - Sample (30 ug of whole cell lysate) A: Jurkat 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/RP105-Antibody-Western-Blot-NBP2-20204-img0001.jpg "Western Blot: RP105/CD180 Antibody [NBP2-20204] - Sample (30 ug of whole cell lysate) A: Jurkat 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: RP105/CD180 Antibody [NBP2-20204] - Mahlarvu xenograft, using RP105 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/RP105-Antibody-Immunohistochemistry-Paraffin-NBP2-20204-img0002.jpg)
![Flow Cytometry: RP105/CD180 Antibody [NBP2-20204] - Flow cytometry on Jurkat cells, staining with RP105 antibody at 1:100 dilution (blue) or rabbit IgG (black).](http://images.novusbio.com/fullsize/RP105-Antibody-Flow-Cytometry-NBP2-20204-img0003.jpg)
![Western Blot: GM-CSF Antibody [NBP2-27161] - Analysis of CSF2 in U937 cell lysate in the (1) absence and (2) presence of immunizing peptide using NBP2-27161 at 3 ug/ml. . Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/GM-CSF-Antibody-Western-Blot-NBP2-27161-img0001.jpg "Western Blot: GM-CSF Antibody [NBP2-27161] - Analysis of CSF2 in U937 cell lysate in the (1) absence and (2) presence of immunizing peptide using NBP2-27161 at 3 ug/ml. . Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP2-19654] - Human breast cancer, using P2X7 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP2-19654-img0002.jpg "Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP2-19654] - Human breast cancer, using P2X7 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: TLR3 Antibody [NBP2-24564] - Analysis of TLR3 in Daudi cell lysate in the (1) absence and (2) presence of immunizing peptide and (3) 3T3 cell lysate using this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/TLR3-Antibody-Western-Blot-NBP2-24564-img0001.jpg "Western Blot: TLR3 Antibody [NBP2-24564] - Analysis of TLR3 in Daudi cell lysate in the (1) absence and (2) presence of immunizing peptide and (3) 3T3 cell lysate using this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: Lysozyme Antibody [NBP1-77790] - Western Blot of Lysozyme antibody. (Left Blot-grey) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Load: 0.5 ug per lane.Primary antibody: Biotin Conjugated Rabbit anti Lysozyme antibody at 1:5000 for overnight at 4C.Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5,000 with Atto 425 conjugated goat anti rabbit secondary antibody at 1:10,000 for 1.5 hrs at RT.(Right Blot-red)Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5,000 with Dylight 549 conjugated secondary antibody at 1:10,000 for 1.5 hrs at RT.Block overnight at 4C.Predicted/Observed size: 4.9kDa, 5kDa for Lysozyme. Other band(s): none.](http://images.novusbio.com/fullsize/Lysozyme-Antibody-Western-Blot-NBP1-77790-img0010.jpg "Western Blot: Lysozyme Antibody [NBP1-77790] - Western Blot of Lysozyme antibody. (Left Blot-grey) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Load: 0.5 ug per lane.Primary antibody: Biotin Conjugated Rabbit anti Lysozyme antibody at 1:5000 for overnight at 4C.Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5,000 with Atto 425 conjugated goat anti rabbit secondary antibody at 1:10,000 for 1.5 hrs at RT.(Right Blot-red)Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5,000 with Dylight 549 conjugated secondary antibody at 1:10,000 for 1.5 hrs at RT.Block overnight at 4C.Predicted/Observed size: 4.9kDa, 5kDa for Lysozyme. Other band(s): none.")
![Western Blot: Lysozyme Antibody [NBP1-77790] - Western Blot of Lysozyme antibody.Lane 1: Lysozyme (Hen Egg White).Load: 50 ng per lane.Primary antibody: Lysozyme antibody at 1:1,000 overnight at 4C.Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 30 min at RT.Block for 30 min at RT.Predicted/Observed size: 4.9 kDa, 4.9 kDa for Lysozyme (Hen Egg White).](http://images.novusbio.com/fullsize/Lysozyme-Antibody-Western-Blot-NBP1-77790-img0009.jpg)
![ELISA: Lysozyme Antibody [NBP1-77790] - ELISA Results of Lysozyme antibody. Each well was coated in duplicate with 1.0 ug of Lysosome. The working dilution is 1:5000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Goat Anti-Rabbit IgG HRP conjugated and TMB substrate.](http://images.novusbio.com/fullsize/Lysozyme-Antibody-ELISA-NBP1-77790-img0011.jpg)
![Western Blot: IL-6 Antibody [NBP1-77894] - Shows detection of recombinant mouse IL-6 raised in E.coli. Recombinant truncated protein (0.1 ug, 21.7 kDa) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-Mouse IL-6 antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4C. After washes, the blot was reacted with secondary antibody DyLight (TM) 649 conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody diluted 1:20,000 in blocking buffer for 30 min. at RT.](http://images.novusbio.com/fullsize/IL-6-Antibody-Western-Blot-NBP1-77894-img0002.jpg "Western Blot: IL-6 Antibody [NBP1-77894] - Shows detection of recombinant mouse IL-6 raised in E.coli. Recombinant truncated protein (0.1 ug, 21.7 kDa) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-Mouse IL-6 antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4C. After washes, the blot was reacted with secondary antibody DyLight (TM) 649 conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody diluted 1:20,000 in blocking buffer for 30 min. at RT.")
![Immunohistochemistry: IL-6 Antibody [NBP1-77894] - Shows nuclear positivity of islets of Langerhans (brown staining) and cytoplasmic staining in mouse pancreas at 10x and 20x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval with E1 (Leica Microsystems) retrieval solution for 20 min and then incubated with rabbit anti-mouse IL-6 antibody at 1:50 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3’3-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.](http://images.novusbio.com/fullsize/IL-6-Antibody-Immunohistochemistry-NBP1-77894-img0004.jpg)
![Western Blot: Granulysin Antibody [NBP2-27101] - Analysis of GNLY using HEK293 cell lysate in the 1) absence and 2) presence of immunizing peptide probed with 2 ug/ml of GNLY antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Granulysin-Antibody-Western-Blot-NBP2-27101-img0001.jpg "Western Blot: Granulysin Antibody [NBP2-27101] - Analysis of GNLY using HEK293 cell lysate in the 1) absence and 2) presence of immunizing peptide probed with 2 ug/ml of GNLY antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: TAB1 Antibody (2A12) [H00010454-M03] - Analysis of MAP3K7IP1 expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/TAB1-Antibody-2A12-Western-Blot-H00010454-M03-img0007.jpg "Western Blot: TAB1 Antibody (2A12) [H00010454-M03] - Analysis of MAP3K7IP1 expression in HeLa (Cat # L013V1).")
![Immunocytochemistry/Immunofluorescence: TAB1 Antibody (2A12) [H00010454-M03] - Analysis of monoclonal antibody to MAP3K7IP1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/TAB1-Antibody-2A12-Immunocytochemistry-Immunofluorescence-H00010454-M03-img0006.jpg)
![Western Blot: TAB1 Antibody (2A12) [H00010454-M03] - Analysis of MAP3K7IP1 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/TAB1-Antibody-2A12-Western-Blot-H00010454-M03-img0008.jpg)
![ELISA: TAB1 Antibody (2A12) [H00010454-M03] - Detection limit for recombinant GST tagged MAP3K7IP1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/TAB1-Antibody-2A12-ELISA-H00010454-M03-img0004.jpg)
![Proximity Ligation Assay: TAB1 Antibody (2A12) [H00010454-M03] - Analysis of protein-protein interactions between HSPA1L and MAP3K7IP1 HeLa cells were stained with anti-HSPA1L rabbit purified polyclonal 1:1200 and anti-MAP3K7IP1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/TAB1-Antibody-2A12-Proximity-Ligation-Assay-H00010454-M03-img0005.jpg)
![Western Blot: IFIT3 Antibody (HL2468) - Azide and BSA Free [NBP3-25515] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IFIT3 antibody [HL2468] (NBP3-25515) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25515_rabbit-ifit3-mab-hl2468-azide-and-bsa-free-2120249374936.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: IFIT3 Antibody (HL2468) - Azide and BSA Free [NBP3-25515] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IFIT3 antibody [HL2468] (NBP3-25515) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: IFIT3 Antibody (HL2468) - Azide and BSA Free [NBP3-25515] - IFIT3 antibody [HL2468] detects IFIT3 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human glioblastoma. IFIT3 stained by IFIT3 antibody [HL2468] (NBP3-25515) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25515_rabbit-ifit3-mab-hl2468-azide-and-bsa-free-212024940380.jpg)
![Immunocytochemistry/Immunofluorescence: IFIT3 Antibody (HL2468) - Azide and BSA Free [NBP3-25515] - IFIT3 antibody [HL2468] detects IFIT3 protein at cytoplasm by immunofluorescent analysis. Sample: MDA-MB-231 cells were fixed in ice-cold MeOH for 5 min. Green: IFIT3 stained by IFIT3 antibody [HL2468] (NBP3-25515) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25515_rabbit-ifit3-mab-hl2468-azide-and-bsa-free-2120249413353.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: IFIT3 Antibody (HL2468) - Azide and BSA Free [NBP3-25515] - Untreated (-) and treated (+) A549 whole cell extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IFIT3 antibody [HL2468] (NBP3-25515) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25515_rabbit-ifit3-mab-hl2468-azide-and-bsa-free-2120249374997.jpg)
![Western Blot: TRIM5 alpha Antibody [NBP1-76601] - Human stomach (A), thymus (B), and uterus (C) cell lysate with TRIM5 alpha antibody at 2 ug /ml.](http://images.novusbio.com/fullsize/TRIM5-alpha-Antibody-Western-Blot-NBP1-76601-img0001.jpg "Western Blot: TRIM5 alpha Antibody [NBP1-76601] - Human stomach (A), thymus (B), and uterus (C) cell lysate with TRIM5 alpha antibody at 2 ug /ml.")
![Immunocytochemistry/ Immunofluorescence: TRIM5 alpha Antibody - BSA Free [NBP1-76601] - Immunofluorescence of TRIM5 alpha in Human Stomach cells with TRIM5 alpha antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76601_rabbit-polyclonal-trim5-alpha-antibody-2711202420324828.jpg)
![Immunohistochemistry: TRIM5 alpha Antibody - BSA Free [NBP1-76601] - Immunohistochemical staining of human stomach using TRIM5 alpha antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76601_rabbit-polyclonal-trim5-alpha-antibody-2711202420312980.jpg)