| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89738-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89884-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12085-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12078-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62562-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09044-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50177-0.025ml | |||
![Flow (Intracellular): Myeloperoxidase/MPO Antibody (MPO421-8B2) [NBP2-41406] - Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human Myeloperoxidase (MPO421-8B2) purified antibody (concentration in sample 1 ug/ml).](http://images.novusbio.com/fullsize/Myeloperoxidase-MPO-Antibody-MPO421-8B2-Flow-Intracellular-NBP2-41406-img0002.jpg "Flow (Intracellular): Myeloperoxidase/MPO Antibody (MPO421-8B2) [NBP2-41406] - Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human Myeloperoxidase (MPO421-8B2) purified antibody (concentration in sample 1 ug/ml).")
![Flow (Intracellular): Myeloperoxidase/MPO Antibody (MPO421-8B2) [NBP2-41406] - Intracellular staining pattern of human peripheral whole blood stained using anti-human Myeloperoxidase (MPO421-8B2) purified antibody (concentration in sample 1 ug/ml).](http://images.novusbio.com/fullsize/Myeloperoxidase-MPO-Antibody-MPO421-8B2-Flow-Intracellular-NBP2-41406-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD55/DAF Antibody (028) [NBP2-89269] - Staining of Human CD55 in JURKAT cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human CD55 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.](http://images.novusbio.com/fullsize/CD55-DAF-Antibody-028-Immunocytochemistry-Immunofluorescence-NBP2-89269-img0002.jpg "Immunocytochemistry/Immunofluorescence: CD55/DAF Antibody (028) [NBP2-89269] - Staining of Human CD55 in JURKAT cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human CD55 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.")
![Flow Cytometry: CD55/DAF Antibody (028) [NBP2-89269] - Analysis of human CD55 expression on K562 cells. Cells were stained with purified anti-Human CD55, then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/CD55-DAF-Antibody-028-Flow-Cytometry-NBP2-89269-img0001.jpg)
![Western Blot: Fc gamma RIIA/CD32a Antibody (JE64-17) [NBP3-33141] - Western blot analysis of Fc gamma RIIA/CD32a on different lysates with Rabbit anti-Fc gamma RIIA/CD32a antibody (NBP3-33141) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: Raji cell lysate Lane 3: HL-60 cell lysate Lane 4: Human lung tissue lysate Cell lysates/proteins at 20 ug/Lane. Tissue lysates/proteins at 30 ug/Lane. Predicted band size: 35 kDa Observed band size: 35-42 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33141) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33141_rabbit-fc-gamma-riia-cd32a-mab-je64-17-28820241423311.jpg "Western Blot: Fc gamma RIIA/CD32a Antibody (JE64-17) [NBP3-33141] - Western blot analysis of Fc gamma RIIA/CD32a on different lysates with Rabbit anti-Fc gamma RIIA/CD32a antibody (NBP3-33141) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: Raji cell lysate Lane 3: HL-60 cell lysate Lane 4: Human lung tissue lysate Cell lysates/proteins at 20 ug/Lane. Tissue lysates/proteins at 30 ug/Lane. Predicted band size: 35 kDa Observed band size: 35-42 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33141) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (JE64-17) [NBP3-33141] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Fc gamma RIIA/CD32a antibody (NBP3-33141) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33141) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33141_rabbit-fc-gamma-riia-cd32a-mab-je64-17-29820241426266.jpg)
![Immunohistochemistry: IgJ Antibody (PD00-89) [NBP3-32462] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-IgJ antibody (NBP3-32462) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32462) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32462_rabbit-igj-mab-pd00-89-298202414304189.jpg "Immunohistochemistry: IgJ Antibody (PD00-89) [NBP3-32462] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-IgJ antibody (NBP3-32462) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32462) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: IgJ Antibody (PD00-89) [NBP3-32462] - Flow cytometric analysis of Daudi cells labeling IgJ. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32462, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32462_rabbit-igj-mab-pd00-89-392024884639.jpg)
![Western Blot: Serpin G1/C1 Inhibitor Antibody (18) [NBP2-89738] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDa Observed band size:71 kDa](http://images.novusbio.com/fullsize/Serpin-G1-C1-Inhibitor-Antibody-18-Western-Blot-NBP2-89738-img0002.jpg "Western Blot: Serpin G1/C1 Inhibitor Antibody (18) [NBP2-89738] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDa Observed band size:71 kDa")
![Immunoprecipitation: Serpin G1/C1 Inhibitor Antibody (18) [NBP2-89738] - Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL anti-SERPING1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody:Anti-SERPING1 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 55 kDa Observed band size: 100 kDa](http://images.novusbio.com/fullsize/Serpin-G1-C1-Inhibitor-Antibody-18-Immunoprecipitation-NBP2-89738-img0001.jpg)
![Flow Cytometry: Clusterin/APOJ Antibody (013) [NBP2-89884] - Analysis of Human CLU on A549 cells. Cells were treated, stained with purified anti-Human CLU, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Clusterin-APOJ-Antibody-013-Flow-Cytometry-NBP2-89884-img0002.jpg "<b>Biological Strategies Validation. </b>Flow Cytometry: Clusterin/APOJ Antibody (013) [NBP2-89884] - Analysis of Human CLU on A549 cells. Cells were treated, stained with purified anti-Human CLU, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Clusterin/APOJ Antibody (013) [NBP2-89884] - Staining of Human CLU in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89884 antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89884_rabbit-monoclonal-clusterin-apoj-antibody-013-235202514371330.png)
![Immunohistochemistry-Paraffin: Complement C3d Antibody (E28-P) [NBP1-79074] - Formalin-fixed and paraffin-embedded human skin biopsy (4 um) from lesion of the early pemphigus vulgaris (without blister formation), stained with anti-C3d complement antibody shows strong positive intraepidermal intercellular immunostaining.](http://images.novusbio.com/fullsize/Complement-C3d-Antibody-E28-P-Immunohistochemistry-Paraffin-NBP1-79074-img0002.jpg "Immunohistochemistry-Paraffin: Complement C3d Antibody (E28-P) [NBP1-79074] - Formalin-fixed and paraffin-embedded human skin biopsy (4 um) from lesion of the early pemphigus vulgaris (without blister formation), stained with anti-C3d complement antibody shows strong positive intraepidermal intercellular immunostaining.")
![Immunocytochemistry/Immunofluorescence: Complement Component C5aR1 Antibody (32-G1) - Chimeric [NBP3-12085] - Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version 32-G1 (NBP3-12085) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12085, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/Complement-Component-C5aR1-Antibody-32-G1-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12085-img0001.jpg "Immunocytochemistry/Immunofluorescence: Complement Component C5aR1 Antibody (32-G1) - Chimeric [NBP3-12085] - Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version 32-G1 (NBP3-12085) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12085, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: Complement Component C5aR1 Antibody (32-G1) - Chimeric - Azide and BSA Free [NBP3-12085] - U937 cells were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12085 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-12085_rabbit-monoclonal-complement-component-c5ar1-antibody-32-g1-chimeric-7420252211300.png)
![Immunocytochemistry/Immunofluorescence: CD59 Antibody (193-27) - Chimeric [NBP3-12078] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 193-27 (NBP3-12078) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12078, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/CD59-Antibody-193-27-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12078-img0001.jpg "Immunocytochemistry/Immunofluorescence: CD59 Antibody (193-27) - Chimeric [NBP3-12078] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 193-27 (NBP3-12078) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12078, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: CD59 Antibody (193-27) - Chimeric - Azide and BSA Free [NBP3-12078] - Jurkat cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12078 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-12078_rabbit-monoclonal-cd59-antibody-193-27-chimeric-74202522141239.png)
![Immunohistochemistry: CD55/DAF Antibody (LU30) - Chimeric [NBP2-62562] - Immunohistochemical staining of human lung tissue using anti-CD55 antibody. LU30 Anti-CD55 (Complement decay-accelerating factor)staining of paraffin embedded human lung tissue using the rabbit-chimeric version of LU30 (NBP2-62562). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62562 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter staining with haematoxylin. Strong staining of the epithelial surface of alveoli may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/CD55-DAF-Antibody-LU30-Chimeric-Immunohistochemistry-NBP2-62562-img0002.jpg "Immunohistochemistry: CD55/DAF Antibody (LU30) - Chimeric [NBP2-62562] - Immunohistochemical staining of human lung tissue using anti-CD55 antibody. LU30 Anti-CD55 (Complement decay-accelerating factor)staining of paraffin embedded human lung tissue using the rabbit-chimeric version of LU30 (NBP2-62562). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62562 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter staining with haematoxylin. Strong staining of the epithelial surface of alveoli may be observed. Recommended concentration, 2-4 ug/ml.")
![Flow Cytometry: CD55/DAF Antibody (LU30) - Chimeric [NBP2-62562] - Flow-cytometry using the anti-CD55 (Complement decay-accelerating factor) antibody LU-30. U93 cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of LU-30 (NBP2-62562, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD55-DAF-Antibody-LU30-Chimeric-Flow-Cytometry-NBP2-62562-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: LAG-3 Antibody (C9B7W) - Chimeric [NBP3-09044] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of C9B7W (NBP3-09044) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09044, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/LAG-3-Antibody-C9B7W-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09044-img0002.jpg "Immunocytochemistry/Immunofluorescence: LAG-3 Antibody (C9B7W) - Chimeric [NBP3-09044] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of C9B7W (NBP3-09044) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09044, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.")
![ELISA: LAG-3 Antibody (C9B7W) - Chimeric - Azide and BSA Free [NBP3-09044] - ELISA Plate coated with recombinant mouse LAG-3 Fc-Fusion Protein at a concentration of 2 ug/ml. A 4-fold serial dilution from 5,000 ng/ml was performed using NBP3-09044. For detection, a 1:4000 dilution of HRP-labelled anti-rabbit antibody was used.](http://images.novusbio.com/fullsize/nbp3-09044_rabbit-monoclonal-lag-3-antibody-c9b7w-chimeric-74202522113011.png)