| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33892-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48701-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-15135-25ul | |||
| Azide and BSA Free | NBP3-44364 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95138-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12068-0.025mg | |||
![Western Blot: Acyloxyacyl Hydrolase Antibody [NBP2-33892] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted)](http://images.novusbio.com/fullsize/Acyloxyacyl-Hydrolase-Antibody-Western-Blot-NBP2-33892-img0005.jpg "Western Blot: Acyloxyacyl Hydrolase Antibody [NBP2-33892] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted)")
![Immunohistochemistry-Paraffin: Acyloxyacyl Hydrolase Antibody [NBP2-33892] - Staining of human duodenum shows strong cytoplasmic positivity in goblet cells.](http://images.novusbio.com/fullsize/Acyloxyacyl-Hydrolase-Antibody-Immunohistochemistry-Paraffin-NBP2-33892-img0006.jpg)
![Immunohistochemistry: Acyloxyacyl Hydrolase Antibody [NBP2-33892] - Staining of human duodenum shows strong positivity in Goblet cells.](http://images.novusbio.com/fullsize/Acyloxyacyl-Hydrolase-Antibody-Immunohistochemistry-NBP2-33892-img0001.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody [NBP2-48701] - Staining of human prostate.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-Immunohistochemistry-Paraffin-NBP2-48701-img0005.jpg "Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody [NBP2-48701] - Staining of human prostate.")
![Immunohistochemistry: Neprilysin/CD10 Antibody [NBP2-48701] - Staining of human small intestine shows membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-Immunohistochemistry-NBP2-48701-img0001.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody [NBP2-48701] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-Immunohistochemistry-Paraffin-NBP2-48701-img0002.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody [NBP2-48701] - Staining in human small intestine and cerebral cortex tissues using anti-MME antibody. Corresponding MME RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-Immunohistochemistry-Paraffin-NBP2-48701-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody [NBP2-48701] - Staining of human cerebral cortex, kidney, prostate and small intestine using Anti-MME antibody NBP2-48701 (A) shows similar protein distribution across tissues to independent antibody NBP2-49050 (B).](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-Immunohistochemistry-Paraffin-NBP2-48701-img0004.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody [NBP2-48701] - Staining of human kidney.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-Immunohistochemistry-Paraffin-NBP2-48701-img0006.jpg)
![Western Blot: TIRAP (TLR2 and TLR4) Antibody [NB300-990] - (1 ug/ml) staining of human liver lysate 35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/TIRAP-TLR2-and-TLR4-Antibody-Western-Blot-NB300-990-img0005.jpg "Western Blot: TIRAP (TLR2 and TLR4) Antibody [NB300-990] - (1 ug/ml) staining of human liver lysate 35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Neprilysin/CD10 Antibody (A1G3-R) [NBP3-32118] - Western blot analysis of Neprilysin/CD10 on different lysates with Mouse anti-Neprilysin/CD10 antibody (NBP3-32118) at 1/2,000 dilution. Lane 1: Daudi cell lysate (20 ug/Lane) Lane 2: Ramos cell lysate (20 ug/Lane) Lane 3: LNCaP cell lysate (20 ug/Lane) Lane 4: HT-29 cell lysate (20 ug/Lane) (negative) Lane 5: Mouse kidney tissue lysate (40 ug/Lane) Predicted band size: 86 kDa Observed band size: 100 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32118) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32118_mouse-neprilysin-cd10-mab-a1g3-r-27820241631626.jpg "Western Blot: Neprilysin/CD10 Antibody (A1G3-R) [NBP3-32118] - Western blot analysis of Neprilysin/CD10 on different lysates with Mouse anti-Neprilysin/CD10 antibody (NBP3-32118) at 1/2,000 dilution. Lane 1: Daudi cell lysate (20 ug/Lane) Lane 2: Ramos cell lysate (20 ug/Lane) Lane 3: LNCaP cell lysate (20 ug/Lane) Lane 4: HT-29 cell lysate (20 ug/Lane) (negative) Lane 5: Mouse kidney tissue lysate (40 ug/Lane) Predicted band size: 86 kDa Observed band size: 100 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32118) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Neprilysin/CD10 Antibody (A1G3-R) [NBP3-32118] - Immunohistochemical analysis of paraffin-embedded human renal clear cell carcinoma tissue with Mouse anti-Neprilysin/CD10 antibody (NBP3-32118) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32118) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32118_mouse-neprilysin-cd10-mab-a1g3-r-298202414161875.jpg)
![Immunocytochemistry/ Immunofluorescence: Neprilysin/CD10 Antibody (A1G3-R) [NBP3-32118] - Immunocytochemistry analysis of Raji cells labeling Neprilysin/CD10 with Mouse anti-Neprilysin/CD10 antibody (NBP3-32118) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Neprilysin/CD10 antibody (NBP3-32118) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32118_mouse-neprilysin-cd10-mab-a1g3-r-29202410484874.jpg)
![Western Blot: TIRAP Antibody (20D1055.1) [NB100-56730] - Analysis of TIRAP in human spleen lysate using this antibody.](http://images.novusbio.com/fullsize/TIRAP-Antibody-20D1055-1-Western-Blot-NB100-56730-img0003.jpg "Western Blot: TIRAP Antibody (20D1055.1) [NB100-56730] - Analysis of TIRAP in human spleen lysate using this antibody.")
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (CL12448) [NBP3-15135] - Staining of human small intestine shows strong apical membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-CL12448-Immunohistochemistry-Paraffin-NBP3-15135-img0004.jpg "Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (CL12448) [NBP3-15135] - Staining of human small intestine shows strong apical membranous positivity in glandular cells.")
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (CL12448) [NBP3-15135] - Staining of human kidney shows strong apical membranous positivity in cells in tubules and cells in glomeruli.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-CL12448-Immunohistochemistry-Paraffin-NBP3-15135-img0001.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (CL12448) [NBP3-15135] - Staining of human pancreas shows very weak positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-CL12448-Immunohistochemistry-Paraffin-NBP3-15135-img0002.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (CL12448) [NBP3-15135] - Staining of human skin shows very weak positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-CL12448-Immunohistochemistry-Paraffin-NBP3-15135-img0003.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (CL12448) [NBP3-15135] - Analysis in human small intestine and pancreas tissues using NBP3-15135 antibody. Corresponding MME RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-CL12448-Immunohistochemistry-Paraffin-NBP3-15135-img0005.jpg)
![Western Blot: Acyloxyacyl Hydrolase Antibody [NBP3-41401] - Sample: Porcine Stomach lysate; Primary Ab: 2ug/ml NBP3-41401; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;](http://images.novusbio.com/fullsize/nbp3-41401_rabbit-acyloxyacyl-hydrolase-pab-61220242116231.jpg "Western Blot: Acyloxyacyl Hydrolase Antibody [NBP3-41401] - Sample: Porcine Stomach lysate; Primary Ab: 2ug/ml NBP3-41401; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;")
![Western Blot: Acyloxyacyl Hydrolase Antibody [NBP3-41401] - Sample: Human Serum; Primary Ab: 2ug/ml NBP3-41401; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;](http://images.novusbio.com/fullsize/nbp3-41401_rabbit-acyloxyacyl-hydrolase-pab-61220242171318.jpg)
![Western Blot: Acyloxyacyl Hydrolase Antibody [NBP3-41401] - Sample: Porcine Spleen lysate; Primary Ab: 2ug/ml NBP3-41401; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;](http://images.novusbio.com/fullsize/nbp3-41401_rabbit-acyloxyacyl-hydrolase-pab-61220242151857.jpg)
![Western Blot: Acyloxyacyl Hydrolase Antibody [NBP3-41401] - Sample: Porcine Small intestine lysate; Primary Ab: 2ug/ml NBP3-41401; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;](http://images.novusbio.com/fullsize/nbp3-41401_rabbit-acyloxyacyl-hydrolase-pab-6122024212747.jpg)
![Western Blot: TIRAP Antibody (9T1A8) [NBP3-16854] - Western blot analysis of extracts of various cell lines, using TIRAP Rabbit mAb (NBP3-16854) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/TIRAP-Antibody-9T1A8-Western-Blot-NBP3-16854-img0003.jpg "Western Blot: TIRAP Antibody (9T1A8) [NBP3-16854] - Western blot analysis of extracts of various cell lines, using TIRAP Rabbit mAb (NBP3-16854) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: TIRAP Antibody (9T1A8) [NBP3-16854] - Immunohistochemistry of paraffin-embedded mouse kidney using TIRAP Rabbit mAb (NBP3-16854) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TIRAP-Antibody-9T1A8-Immunohistochemistry-Paraffin-NBP3-16854-img0002.jpg)
![Immunohistochemistry-Paraffin: TIRAP Antibody (9T1A8) [NBP3-16854] - Immunohistochemistry of paraffin-embedded rat lung using TIRAP Rabbit mAb (NBP3-16854) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TIRAP-Antibody-9T1A8-Immunohistochemistry-Paraffin-NBP3-16854-img0001.jpg)
![Western Blot: Neprilysin/CD10 Antibody (5E9A5) [NBP2-37301] - Western blot analysis using CD10 mAb against HEK293 (1) and CD10 (AA: 52-246)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-5E9A5-Western-Blot-NBP2-37301-img0003.jpg "Western Blot: Neprilysin/CD10 Antibody (5E9A5) [NBP2-37301] - Western blot analysis using CD10 mAb against HEK293 (1) and CD10 (AA: 52-246)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry: Neprilysin/CD10 Antibody (5E9A5) [NBP2-37301] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CD10 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-5E9A5-Immunohistochemistry-NBP2-37301-img0004.jpg)
![Western Blot: Neprilysin/CD10 Antibody (5E9A5) [NBP2-37301] - Western blot analysis using CD10 mAb against human CD10 (AA: 52-246) recombinant protein. (Expected MW is 41.4 kDa)](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-5E9A5-Western-Blot-NBP2-37301-img0002.jpg)
![ELISA: Neprilysin/CD10 Antibody (5E9A5) [NBP2-37301] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-5E9A5-ELISA-NBP2-37301-img0001.jpg)
![Western Blot: TIRAP Antibody [NBP2-95138] - Analysis of extracts of various cell lines, using TIRAP at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.](http://images.novusbio.com/fullsize/TIRAP-Antibody-Western-Blot-NBP2-95138-img0005.jpg "Western Blot: TIRAP Antibody [NBP2-95138] - Analysis of extracts of various cell lines, using TIRAP at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.")
![Immunocytochemistry/ Immunofluorescence: TIRAP Antibody - BSA Free [NBP2-95138] - Immunofluorescence analysis of U2OS cells using TIRAP antibody (A12606) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95138_rabbit-polyclonal-tirap-antibody-5220251931353.jpg)
![Western Blot: Neprilysin/CD10 Antibody (FR4D11) - Chimeric [NBP3-12068] - Daudi cell lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of FR4D11 (NBP3-12068) at 2 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-FR4D11-Chimeric-Western-Blot-NBP3-12068-img0001.jpg "Western Blot: Neprilysin/CD10 Antibody (FR4D11) - Chimeric [NBP3-12068] - Daudi cell lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of FR4D11 (NBP3-12068) at 2 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry /Immunofluorescence: Neprilysin/CD10 Antibody (FR4D11) - Chimeric - Azide and BSA Free [NBP3-12068] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix(TM) coverslips stained with NBP3-12068 at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12068, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-12068_rabbit-monoclonal-neprilysin-cd10-antibody-fr4d11-chimeric-74202522141215.png)