| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85741-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71021 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37265-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37291-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25241SS | |||
| Azide and BSA Free | NBP2-80965 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87781-25ul | |||
![Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody [NBP1-85741] - Analysis in human placenta and pancreas tissues. Corresponding TfR (Transferrin R) RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-Immunohistochemistry-Paraffin-NBP1-85741-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody [NBP1-85741] - Analysis in human placenta and pancreas tissues. Corresponding TfR (Transferrin R) RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: TfR (Transferrin R) Antibody [NBP1-85741] - Staining of human cell line U-251 MG shows localization to endosomes and lysosomes. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85741-img0014.jpg)
![Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody [NBP1-85741] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-Immunohistochemistry-Paraffin-NBP1-85741-img0019.jpg)
![Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody [NBP1-85741] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-Immunohistochemistry-Paraffin-NBP1-85741-img0016.jpg)
![Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody [NBP1-85741] - Staining of human lung shows strong cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-Immunohistochemistry-Paraffin-NBP1-85741-img0017.jpg)
![Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody [NBP1-85741] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-Immunohistochemistry-Paraffin-NBP1-85741-img0018.jpg)
![Western Blot: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of extracts (10ug) from 5 different cell lines by using anti-IL1A monoclonal antibody. (1:200)](http://images.novusbio.com/fullsize/IL-1-alpha-IL-1F1-Antibody-2F8-Western-Blot-NBP2-45400-img0008.jpg "Western Blot: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of extracts (10ug) from 5 different cell lines by using anti-IL1A monoclonal antibody. (1:200)")
![Immunocytochemistry/Immunofluorescence: IL-1 alpha/IL-1F1 Antibody (OTI2F8) [NBP2-45400] - IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY IL-1 alpha.](http://images.novusbio.com/fullsize/IL-1-alpha-IL-1F1-Antibody-2F8-Immunofluorescence-NBP2-45400-img0001.jpg)
![Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/IL-1-alpha-IL-1F1-Antibody-2F8-Immunohistochemistry-NBP2-45400-img0006.jpg)
![Western Blot: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of HEK293T cells lysate (5 ug, left lane) and full length human recombinant protein of human IL-1 alpha (NP_000566) produced in Analysis of HEK293T cell (5 ug, right lane).](http://images.novusbio.com/fullsize/IL-1-alpha-IL-1F1-Antibody-2F8-Western-Blot-NBP2-45400-img0007.jpg)
![Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/IL-1-alpha-IL-1F1-Antibody-2F8-Immunohistochemistry-NBP2-45400-img0002.jpg)
![Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/IL-1-alpha-IL-1F1-Antibody-2F8-Immunohistochemistry-NBP2-45400-img0003.jpg)
![Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/IL-1-alpha-IL-1F1-Antibody-2F8-Immunohistochemistry-NBP2-45400-img0004.jpg)
![Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/IL-1-alpha-IL-1F1-Antibody-2F8-Immunohistochemistry-NBP2-45400-img0005.jpg)
![Western Blot: COX-1 Antibody [NB100-867] - Staining (1ug/ml) of U937 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/COX-1-Antibody-Western-Blot-NB100-867-img0010.jpg "Western Blot: COX-1 Antibody [NB100-867] - Staining (1ug/ml) of U937 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: COX-1 Antibody [NB100-867] - Staining (3.8µg/ml) of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-Staining.](http://images.novusbio.com/fullsize/COX-1-Antibody-Immunohistochemistry-Paraffin-NB100-867-img0009.jpg)
![Immunohistochemistry: COX-1 Antibody [NB100-867] - Immunohistochemistry and Virtual Channel confocal microscopy of IL-4 and IL-13 treated enteroids from distal ileum of C57BL/6 J mice, show co-localization of advillin (green), DCLK1 (red) and PTGS1/COX-1 (magenta) within the same cells identified as tuft cells. Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-020-65469-0), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/COX-1-Antibody-Immunohistochemistry-NB100-867-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: COX-1 Antibody [NB100-867] - Intestinal tuft cells express advillin. (a) Immunohistochemistry of paraffin-embedded tissue from distal ileum of C57BL/6 J mice using advillin (green) & DCLK1 (red) antibodies. Nuclei are counter stained with DAPI. Both proteins co-localize in the same cell. Note that not all DCLK1 positive cells are positive for advillin expression. Right panel shows higher magnification of boxed area & shows co-localization of DCLK1 & advillin in a tuft cell. (b) Immunohistochemistry of paraffin embedded tissue from distal ileum of C57BL/6 J mice using advillin (green) & PTGS1 (red) antibodies. Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area & co-localization of PTGS1 & advillin in a tuft cell. (c) Immunohistochemistry of cryopreserved tissue from distal ileum of C57BL/6 J mice using Alexa Fluor 568 Phalloidin (red) & advillin (green). Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area & co-localization of advillin & F-actin at the apical surface of a tuft cell. Data shown are representative of n = 5 animals. Scale bar represents 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32483224), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-867_goat-polyclonal-cox-1-antibody-31020241628497.jpg)
![Immunocytochemistry/ Immunofluorescence: COX-1 Antibody [NB100-867] - In intestinal enteroids, tuft cells express advillin. (a) Immuno-histochemistry of enteroids from distal ileum of C57BL/6 J mice shows tuft cell hyperplasia 72 hours post IL-4 & IL-13 treatment. Control refers to untreated enteroids from C57BL/6 J mice. Advillin (green) & DCLK1 (red) co-localization was used to identify tuft cells. Nuclei are counter stained with DAPI. Right panel shows higher magnification of the boxed area. (b) Immunohistochemistry of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice, show co-localization in tuft cells of advillin (green) & DCLK1 (red) in the upper panel; & advillin & PTGS1 (red) in the lower panel. Nuclei were counter stained with DAPI. Right panels show higher magnification of boxed area. Data shown are representative of n = 5 animals. Scale bar represents 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32483224), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-867_goat-polyclonal-cox-1-antibody-31020241628492.jpg)
![Immunocytochemistry/ Immunofluorescence: COX-1 Antibody [NB100-867] - Tuft cells positive for advillin are negative for villin. (a) Immunohistochemistry & Virtual Channel confocal microscopy of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice, show co-localization of advillin (green), DCLK1 (red) & PTGS1 (magenta) within the same cells identified as tuft cells. Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area. (b) Immunohistochemistry of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice show co-localization in tuft cells of advillin (green) with F-actin (red) in the upper panel; & co-localization of advillin (red) & tubulin (green) in the lower panel. Nuclei were counter stained with DAPI. Right panel shows higher magnification of boxed area. Note the co-localization of advillin to the apical tuft that terminates near the perinuclear region of the cells. Advillin appears in vesiculated structures that co-localize with tubulin near the lateral cell surface & in the cytoplasm. (c) Immunohistochemistry of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice show the absence of villin (red) from advillin (green) expressing tuft cells. Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area. Data shown are representative of n = 5 animals. Scale bar represents 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32483224), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-867_goat-polyclonal-cox-1-antibody-310202416284923.jpg)
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining pattern of human peripheral blood stained using anti-human CD28 (CD28.2) purified antibody (concentration in sample 1,6 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0009.jpg "Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining pattern of human peripheral blood stained using anti-human CD28 (CD28.2) purified antibody (concentration in sample 1,6 ug/ml) GAM APC.")
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) purified.](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0001.jpg)
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) PE.](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0002.jpg)
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) PerCP-CyTM5.5](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0003.jpg)
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) APC.](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0004.jpg)
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) PerCP.](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0005.jpg)
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) FITC.](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0006.jpg)
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) PE-CyTM7.](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0007.jpg)
![Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Separation of human CD28 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD28 (CD28.2) purified antibody (concentration in sample 1,6 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/CD28-Antibody-CD28-2-Flow-Cytometry-NBP1-79126-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Immunofluorescence analysis of Hela cells using CD68 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-3F7D3-Immunocytochemistry-Immunofluorescence-NBP2-37265-img0005.jpg "Immunocytochemistry/Immunofluorescence: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Immunofluorescence analysis of Hela cells using CD68 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.")
![Western Blot: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Western blot analysis using CD68 mouse mAb against U937 (1), Hela (2), HepG2 (3), Jurkat (4) cell lysate.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-3F7D3-Western-Blot-NBP2-37265-img0004.jpg)
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Analysis of bladder cancer tissues using CD68 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-3F7D3-Immunohistochemistry-NBP2-37265-img0008.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Flow cytometric analysis of Hela cells using CD68 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-3F7D3-Flow-Cytometry-NBP2-37265-img0006.jpg)
![Western Blot: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Western blot analysis using CD68 mAb against human CD68 (AA: 42-155) recombinant protein. (Expected MW is 37.4 kDa)](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-3F7D3-Western-Blot-NBP2-37265-img0002.jpg)
![Western Blot: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Western blot analysis using CD68 mAb against HEK293 (1) and CD68 (AA: 42-155)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-3F7D3-Western-Blot-NBP2-37265-img0003.jpg)
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Analysis of endometrial cancer tissues using CD68 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-3F7D3-Immunohistochemistry-NBP2-37265-img0007.jpg)
![ELISA: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-3F7D3-ELISA-NBP2-37265-img0001.jpg)
![Dual RNAscope ISH-IHC: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Tissue sections of human tonsil were probed for CD14 mRNA (ACD RNAScope probe, catalog # 418801; Fast Red chromogen, ACD catalog # 322500). Adjacent tissue section was processes for immunohistochemistry using mouse monoclonal (Novus catalog # NBP2-37291) at 1:100 dilution for 1 hour at room temperature followed by incubation with the anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue).](http://images.novusbio.com/fullsize/CD14-Antibody-4B4F12-Dual-RNAscope-ISH-IHC-NBP2-37291-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Tissue sections of human tonsil were probed for CD14 mRNA (ACD RNAScope probe, catalog # 418801; Fast Red chromogen, ACD catalog # 322500). Adjacent tissue section was processes for immunohistochemistry using mouse monoclonal (Novus catalog # NBP2-37291) at 1:100 dilution for 1 hour at room temperature followed by incubation with the anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue).")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Confocal image of hCD14-transfected HEKT cells, 4% formalin fixed, indirectly labeled with anti-CD14 1:200, and AF568-conjugated Ab 1:1000. Note that cells have different immunoreactivity due to various expression level of CD14 in single cells. Image from verified customer review.](http://images.novusbio.com/fullsize/CD14-Antibody-4B4F12-Immunocytochemistry-NBP2-37291-img0007.jpg)
![Flow Cytometry: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - An intracellular stain was performed on Jurkat cells with NBP2-37291AF647 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/CD14-Antibody-4B4F12-Flow-Cytometry-NBP2-37291-img0008.jpg)
![Western Blot: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Analysis using CD14 mAb against human CD14 (AA: 20-214) recombinant protein. (Expected MW is 46.8 kDa)](http://images.novusbio.com/fullsize/CD14-Antibody-4B4F12-Western-Blot-NBP2-37291-img0002.jpg)
![Western Blot: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Analysis using CD14 mouse mAb against HepG2 (1), A549 (2), HL60 (3), RAW264.7 (4), Hela (5), HEK293 (6) and NIH/3T3 (7) cell lysate.](http://images.novusbio.com/fullsize/CD14-Antibody-4B4F12-Western-Blot-NBP2-37291-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - BMDM were unchallenged (control) or challenged with 500 HU/mL SLO WT, PFO or an equivalent mass of SLO ML for 10 min at 37C and centrifuged at 2000 x g for 5 min to yield cell pellet (C). Supernatants were spun at 100,000xg for 40 min at 4C to collect the high speed supernatant (S) and microvesicle pellet (M). Samples were solubilized at 95C in SDS-sample buffer, resolved by SDS-PAGE and transferred to nitrocellulose. Portions of the blot were probed with 6D11 anti-SLO, anti-IFNgammaR1, 4B4F12 anti-CD14, 76B357.1 anti-TLR4, O91B8 anti-MyD88, 1H4B01 anti-Trif, EPR4477 anti-Alkaline Phosphatase, MANLAC-4A7 anti-Lamin A/C, and AC-15 anti-beta-Actin, followed by relevant secondary antibodies and ECL. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-24955-2) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD14-Antibody-4B4F12-Western-Blot-NBP2-37291-img0010.jpg)
![Western Blot: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Analysis using CD14 mAb against HEK293 (1) and CD14 (AA: 20-214)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CD14-Antibody-4B4F12-Western-Blot-NBP2-37291-img0003.jpg)
![ELISA: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CD14-Antibody-4B4F12-ELISA-NBP2-37291-img0001.jpg)
![Western Blot: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of CD34 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: TF-1 cell lysate Lane 2: Mouse lung tissue lysate](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Western-Blot-NBP2-67399-img0007.jpg "Western Blot: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of CD34 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: TF-1 cell lysate Lane 2: Mouse lung tissue lysate")
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - (G) CD34 + microvascular staining in EOC tissue. Correlation between HE4 serum levels and CD34 + area in patient EOC tissue (n = 14). **p < 0.005, ****p < 0.0005. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-020-65353-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0008.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded rat kidney tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0003.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0004.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded mouse kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0006.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human tonsil tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0005.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human tonsil tissue labeling CD34 with Rabbit anti-CD34 antibody washed with PBS, and then probed with the primary antibody green) at 1/50 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0009.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0010.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human placenta tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0011.jpg)
![Western Blot: Dicer Antibody [NBP1-06520] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiDicer antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Dicer-Antibody-Western-Blot-NBP1-06520-img0003.jpg "Western Blot: Dicer Antibody [NBP1-06520] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiDicer antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunoprecipitation: Dicer Antibody [NBP1-06520] - Detection of human Dicer by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Dicer antibody NBP1-06520 (lot NBP1-06520-5) used for IP at 6 ug per reaction. Dicer was also immunoprecipitated by a previous lot of this antibody (lot NBP1-06520-4). For blotting immunoprecipitated Dicer, NBP1-06520 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunoprecipitation-NBP1-06520-img0004.jpg)
![Western Blot: Dicer Antibody [NBP1-06520] - Nef does not affect siRNA loading into RISC, but inhibits its slicing activity.(A) Lysates from the U937 stable cell lines were prepared in Buffer D as described in Materials & Methods. From this, 25 µg of lysate was incubated with 32P-labeled duplex siRNA (without or with 50-fold molar excess of unlabeled siRNA) in binding buffer for 30 min at 4°C. The complexes were separated on a 6% non-denaturing polyacrylamide gel. (B) The gel shift assay was set up as in (A) except that lysates immunodepleted for either Ago2 or Dicer were also included. The positions of the mobility shifted complexes & free probe are indicated. (C) Slicer activity was assayed using immunoprecipitated Ago2 from U937/Nef-EYFP or U937/EYFP cell lysates & a 32P-labeled in vitro transcribed let7a RNA, as described in Materials & Methods. The positions of full-length RNA & the two sliced products are shown. The 59 nt marker oligonucleotide was run on the same gel, but a lower exposure is shown. (D) Densitometric analysis of autoradiograms from three independent experiments was carried out using Image J (version 1.4.1). The slicing activity in Nef-EYFP lysates is represented as a percentage of that in EYFP lysates. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0074472), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-06520_rabbit-polyclonal-dicer-antibody-310202416175285.jpg)
![Immunohistochemistry: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - Staining of human lung frozen section.](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-122D2-08-Immunohistochemistry-DDX0380P-100-img0005.jpg "Immunohistochemistry: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - Staining of human lung frozen section.")
![Flow Cytometry: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - FACS staining of swine DC in skin and offerent lymph](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-122D2-08-Flow-Cytometry-DDX0380P-100-img0007.jpg)
![Immunohistochemistry-Paraffin: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - Expression of C-type lectin receptors on human skin](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-122D2-08-Immunohistochemistry-Paraffin-DDX0380P-100-img0004.jpg)
![Flow Cytometry: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - FACS staining of human in vitro generated DC's](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-122D2-08-Flow-Cytometry-DDX0380P-100-img0006.jpg)
![In-situ Hybridization: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - In vitro staining of swine skin DCS](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-122D2-08-In-situ-Hybridization-DDX0380P-100-img0008.jpg)
![Western Blot: STAT6 Antibody (177C322.1) [NBP2-25241] - Analysis of STAT6 in HeLa lysate in the A) absence and B) presence of immunizing peptide using STAT6 antibody at 2 ug/ml. anti mouse Ig HRP secondary antibody and ECL substrate were used for this test.](http://images.novusbio.com/fullsize/STAT6-Antibody-177C322-1-Western-Blot-NBP2-25241-img0001.jpg "Western Blot: STAT6 Antibody (177C322.1) [NBP2-25241] - Analysis of STAT6 in HeLa lysate in the A) absence and B) presence of immunizing peptide using STAT6 antibody at 2 ug/ml. anti mouse Ig HRP secondary antibody and ECL substrate were used for this test.")
![Immunocytochemistry/Immunofluorescence: STAT6 Antibody (177C322.1) [NBP2-25241] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-STAT6 (177C322.1) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/STAT6-Antibody-177C322-1-Immunocytochemistry-Immunofluorescence-NBP2-25241-img0003.jpg)
![Western Blot: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/RelA/NFkB p65 p65 antibody (27F9.G4) was used to detect p65 by Western blot. Samples were prepared in RIPA lysis buffer, boiled with NuPage 4x LDS Sample Buffer and run on NuPage 4-12% Bis-Tris Gels. Blot was incubated with primary antibody at a dilution of 1:500 and detected with HRP conjugated anti-mouse antibody at a dilution of 1:10000.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Western-Blot-NBP1-77815-img0018.jpg "Western Blot: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/RelA/NFkB p65 p65 antibody (27F9.G4) was used to detect p65 by Western blot. Samples were prepared in RIPA lysis buffer, boiled with NuPage 4x LDS Sample Buffer and run on NuPage 4-12% Bis-Tris Gels. Blot was incubated with primary antibody at a dilution of 1:500 and detected with HRP conjugated anti-mouse antibody at a dilution of 1:10000.")
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Tissue: Human Fibroblasts Top: Before activation Bottom: After activation with poly IC Left: anti p65 NLS specific Right: Monoclonal antibody C-Term The two antibodies that are shown target different regions of the p65 protein. The different staining patterns are thought to correspond with different functional regions of the protein.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0020.jpg)
![Immunohistochemistry: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. Showed moderate to strong staining within many tissues, including epithelium of the breast, small intestine, kidney, pancreas, prostate, skin, placenta, and uterus, as well as within neurons and lymphoid tissues such as spleen, thymus, and tonsil. The antibody produced an excellent signal with almost no background staining at a concentration of 2.5ug/ml. The image displayed shows specific staining in colon carcinoma as the precipitated red signal, with a hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunohistochemistry-NBP1-77815-img0019.jpg)
![Western Blot: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/NFkB p65 antibody (27F9.G4) was used to detect ~65 kD band (red arrow) in HeLa whole cell lysate. Lysate was run on 4-20% gradient gel transferred under standard conditions and blocked in 1% BSA-TBST for 30 min at RT. Blot was probed with monoclonal anti-NFkB p65 at 1:1000 in 1% BSA-TBST at 4C and detected with HRP conjugated Rb-anti-Mouse antibody at 1:40,000 in for 30 min at RT.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Western-Blot-NBP1-77815-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Used at a dilution of 1:5000. Hela cells were grown to sub-confluent on 18 mm2 glass coverslips #1.5. Cells were either unstimulated (A), or stimulated (B) with 50 ng/ml of TNF alpha for 30 min prior fixation. Cells were then fixed in methanol and blocked with 10% normal goat serum (NGS), in PBS, and TritonX 0.2% (Tx) and incubated for 1 hr at RT with primary ab, counterstained with DAPI and washed in PBS/NGS/Tx. Cells were incubated for 1 hr at RT with Atto 425 conjugated anti mouse secondary antibody for STED CW imaging.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Mouse endothelial cells. Unstimulated control cells (left) show cytoplasmic staining, TNF-alpha stimulated cells (right) show nuclear staining. For staining, cells were washed with PBS to remove all traces of culture media and fixed with paraformaldehyde 4 % (45 min). Slides were washed with PBT (phosphate buffer 0.1M + Triton-X-100 0.1 %), 3 times 10 min each, then, blocked with PBT + 5 % normal goat serum, 1 hour. Sample was Incubated overnight in primary antibody (1:600 in blocking buffer). After 3X wash in PBT for 10 min, slides were incubated 1 hour with secondary antibody (1:1000) and mounted in 1:1 PB glycerol.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/NFkB p65 antibody (27F9.G4) was used to detect p65 by immunofluorescence at a dilution of 1:5000. Hela cells were grown to sub-confluent on 18 mm2 glass coverslips #1.5. Cells were either unstimulated (A), or stimulated (B) with 50 ng/ml of TNF alpha for 30 min prior fixation. Cells were then fixed in methanol and blocked with 10% normal goat serum (NGS), in PBS, and TritonX 0.2% (Tx) and incubated for 1 hr at RT with primary ab, counterstained with DAPI and washed in PBS/NGS/Tx. Cells were incubated for 1 hr at RT with Atto 425 conjugated anti mouse secondary antibody for STED CW imaging. Data was collected on a STED-CW TCS-SP5 Confocal system equipped with a DFC 350FX camera allowing sequential acquisition in widefiled, confocal and STED CW imaging on the same system.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0015.jpg)
![Immunohistochemistry-Paraffin: Myelin PLP Antibody [NBP1-87781] - Staining in human cerebral cortex and pancreas tissues using anti-PLP1 antibody. Corresponding PLP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Myelin-PLP-Antibody-Immunohistochemistry-Paraffin-NBP1-87781-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Myelin PLP Antibody [NBP1-87781] - Staining in human cerebral cortex and pancreas tissues using anti-PLP1 antibody. Corresponding PLP1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Myelin PLP Antibody [NBP1-87781] - Border of a demyelinated lesion. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Myelin-PLP-Antibody-Immunohistochemistry-Paraffin-NBP1-87781-img0013.jpg)
![Immunohistochemistry-Paraffin: Myelin PLP Antibody [NBP1-87781] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Myelin-PLP-Antibody-Immunohistochemistry-Paraffin-NBP1-87781-img0010.jpg)
![Immunohistochemistry-Paraffin: Myelin PLP Antibody [NBP1-87781] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Myelin-PLP-Antibody-Immunohistochemistry-Paraffin-NBP1-87781-img0011.jpg)
