| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36567SS | |||
| BSA Free | NBP2-36567 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74186 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-610-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-62994-0.2mg | |||
| BSA Free | NB100-62994-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05030-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38538-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37388-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87485-25ul | |||
![Western Blot: CD63 Antibody (7G4.2E8) - Azide and BSA Free [NBP2-80653] - Western blot analysis of mouse CD63 on recombinant mouse CD63 and mouse kidney lysate. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/CD63-Antibody-7G4-2E8-Azide-and-BSA-Free-Western-Blot-NBP2-80653-img0001.jpg "Western Blot: CD63 Antibody (7G4.2E8) - Azide and BSA Free [NBP2-80653] - Western blot analysis of mouse CD63 on recombinant mouse CD63 and mouse kidney lysate. Image from the standard format of this antibody.")
![Flow Cytometry: CD63 Antibody (7G4.2E8) - Azide and BSA Free [NBP2-80653] - Intracellular staining of mouse CD63 in Flow cytometry using 2.0 ug of antibody per 1 million cells. Isotype control was rat IgG2a lambda. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/CD63-Antibody-7G4-2E8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80653-img0002.jpg)
![Western Blot: Siglec-2/CD22 Antibody (1F12) [NBP2-46125] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD22.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-1F12-Western-Blot-NBP2-46125-img0003.jpg "Western Blot: Siglec-2/CD22 Antibody (1F12) [NBP2-46125] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD22.")
![Immunocytochemistry/Immunofluorescence: Siglec-2/CD22 Antibody (OTI1F12) [NBP2-46125] - Staining in canine blood cells from a verified customer review.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-OTI1F12-Immunocytochemistry-Immunofluorescence-NBP2-46125-img0005.jpg)
![Western Blot: Siglec-2/CD22 Antibody (1F12) [NBP2-46125] - Analysis of extracts (35ug) from 9 different cell lines by using CD22 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-1F12-Western-Blot-NBP2-46125-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Siglec-2/CD22 Antibody (OTI1F12) [NBP2-46125] - Siglec-2/CD22 Antibody (1F12) [NBP2-46125] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY CD22.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-1F12-Immunofluorescence-NBP2-46125-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Siglec-2/CD22 Antibody (OTI1F12) [NBP2-46125] - Siglec-2/CD22 Antibody (1F12) [NBP2-46125] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-1F12-Immunofluorescence-NBP2-46125-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - A-D Confocal microscopy of IBA-1 (green staining) immunohistochemistry of RPE flatmounts (RPE autofluorescence visible as orange due to its autofluorescence in the red and green channel) from a healthy donor (A), a geographic atrophy lesion (B), and large drusen (C and D). (A, B, D): orthogonal Z-stack projection; (C): oblique Z-stack projection and dissecting microscope appearance of postmortem large drusen after removal of the overlaying retina (inset). E-G Double-labeling on the subretinal side of the retina (to avoid masking by RPE autofluorescence) of IBA-1+ (E, green fluorescence) and CD18 (F, red fluorescence; G, merge). H. Orthogonal and lateral Z-stack of a subretinal IBA-1+ (green fluorescence) MPs adjacent to the RPE (orange autofluorescence) in the vicinity of a large drusen.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Immunocytochemistry-Immunofluorescence-NB200-610-img0011.jpg "Immunocytochemistry/Immunofluorescence: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - A-D Confocal microscopy of IBA-1 (green staining) immunohistochemistry of RPE flatmounts (RPE autofluorescence visible as orange due to its autofluorescence in the red and green channel) from a healthy donor (A), a geographic atrophy lesion (B), and large drusen (C and D). (A, B, D): orthogonal Z-stack projection; (C): oblique Z-stack projection and dissecting microscope appearance of postmortem large drusen after removal of the overlaying retina (inset). E-G Double-labeling on the subretinal side of the retina (to avoid masking by RPE autofluorescence) of IBA-1+ (E, green fluorescence) and CD18 (F, red fluorescence; G, merge). H. Orthogonal and lateral Z-stack of a subretinal IBA-1+ (green fluorescence) MPs adjacent to the RPE (orange autofluorescence) in the vicinity of a large drusen.")
![Immunohistochemistry-Frozen: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Canine histiocytic sarcoma stained with Integrin beta 2/CD18 Antibody (YFC118.3). IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Immunohistochemistry-Frozen-NB200-610-img0018.jpg)
![Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Staining of human peripheral blood granulocytes with RAT ANTI HUMAN CD18: FITC.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Flow-Cytometry-NB200-610-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Analysis of canine thoracic fluid samples using anti-Integrin beta 2 antibody. The antibody showing positive cell surface staining. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Immunocytochemistry-Immunofluorescence-NB200-610-img0007.jpg)
![Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Staining of human peripheral blood lymphocytes with Rat anti Human CD18.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Flow-Cytometry-NB200-610-img0001.jpg)
![Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) or CD11b (C) before incubation with Leishmania. D and E represent expression of CD18 (D) or CD11b (E) after association with Leishmania in a SD assay. In all cases the percent positive cells within the M1 marker are identified. This Figure is a representative diagram of 4 experiments with similar findings.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Flow-Cytometry-NB200-610-img0008.jpg)
![Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - LFA-1 activation analyses by Flow Cytometry. (A) Profiles of CD11a, CD18 activation epitope (CD18act, representing high affinity conformation) and CD18 (CD18tot) expression by Teff and Treg#1 cells pre-incubated or not with indicated antibodies. Anti-CD28 and anti-CTLA-4 were used at 10 ug/ml. (B) Histograms of Mean Fluorescent Intensity (MFI) of CD11a, CD18act and CD18tot expressed on Teff and Treg#1 cells. Ratio CD18act MFI: CD18tot was established to analyze LFA-1 high affinity conformation in indicated conditions. Data are representative of more than three different experiments. Filled gray, negative control; Red line, Teff and Treg cells alone; Green line, cells with APC; Blue line, cells with APC and anti-CD28; and Black line: cells with APC, anti-CD28 and anti-CTLA-4.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Flow-Cytometry-NB200-610-img0015.jpg)
![Flow Cytometry: CD14 Antibody (11-248.2) [NB100-62994] - Staining of human peripheral blood monocytes with Mouse anti Human CD14.](http://images.novusbio.com/fullsize/CD14-Antibody-11-248-2-Flow-Cytometry-NB100-62994-img0016.jpg "Flow Cytometry: CD14 Antibody (11-248.2) [NB100-62994] - Staining of human peripheral blood monocytes with Mouse anti Human CD14.")
![Flow Cytometry: CD14 Antibody (11-248.2) [NB100-62994] Staining of human peripheral blood monocytes with Mouse anti Human CD14](http://images.novusbio.com/fullsize/CD14-Antibody-11-248-2-Flow-Cytometry-NB100-62994-img0009.jpg)
![Flow Cytometry: CD14 Antibody (11-248.2) [NB100-62994] - Staining of human peripheral blood granulocytes with Mouse anti Human CD14.](http://images.novusbio.com/fullsize/CD14-Antibody-11-248-2-Flow-Cytometry-NB100-62994-img0010.jpg)
![Flow Cytometry: CD14 Antibody (11-248.2) [NB100-62994] - Staining of human peripheral blood monocytes probed with Mouse anti Human CD14.](http://images.novusbio.com/fullsize/CD14-Antibody-11-248-2-Flow-Cytometry-NB100-62994-img0011.jpg)
![Flow Cytometry: CD14 Antibody (11-248.2) [NB100-62994] - Staining of human peripheral blood monocytes with Mouse anti Human CD14.](http://images.novusbio.com/fullsize/CD14-Antibody-11-248-2-Flow-Cytometry-NB100-62994-img0012.jpg)
![Flow Cytometry: CD14 Antibody (11-248.2) [NB100-62994] - Staining of human peripheral blood monocytes with Mouse anti Human CD14.](http://images.novusbio.com/fullsize/CD14-Antibody-11-248-2-Flow-Cytometry-NB100-62994-img0013.jpg)
![Flow Cytometry: CD14 Antibody (11-248.2) [NB100-62994] - Staining of human peripheral blood monocytes with Mouse anti Human CD14.](http://images.novusbio.com/fullsize/CD14-Antibody-11-248-2-Flow-Cytometry-NB100-62994-img0014.jpg)
![Flow Cytometry: CD14 Antibody (11-248.2) [NB100-62994] - Staining of human peripheral blood monocytes with Mouse anti Human CD14.](http://images.novusbio.com/fullsize/CD14-Antibody-11-248-2-Flow-Cytometry-NB100-62994-img0015.jpg)
![Western Blot: Fc gamma RII/CD32 Antibody [NB100-1532] - (1ug/ml) staining of Daudi cell lysate (RIPA buffer, (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Fc-gamma-RII-CD32-Antibody-Western-Blot-NB100-1532-img0006.jpg "Western Blot: Fc gamma RII/CD32 Antibody [NB100-1532] - (1ug/ml) staining of Daudi cell lysate (RIPA buffer, (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Fc gamma RII/CD32 Antibody [NB100-1532] - Staining (8ug/ml) of paraffin embedded Human Placenta. Heat induced antigen retrieval with citrate buffer pH 6, HRP-Staining.](http://images.novusbio.com/fullsize/Fc-gamma-RII-CD32-Antibody-Immunohistochemistry-Paraffin-NB100-1532-img0007.jpg)
![Immunohistochemistry-Paraffin: Fc gamma RII/CD32 Antibody [NB100-1532] - Negative Control showing staining of paraffin embedded Human Placenta, with no primary antibody.](http://images.novusbio.com/fullsize/Fc-gamma-RII-CD32-Antibody-Immunohistochemistry-Paraffin-NB100-1532-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Goat Polyclonal Fc gamma RII/CD32 Antibody [NB100-1532] - Immunofluorescence analysis of paraformaldehyde fixed THP-1 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/antibody/nb100-1532_goat-polyclonal-fc-gamma-rii-cd32-antibody-immunocytochemistry-immunofluorescence-2832024113032..jpg)
![Flow Cytometry: CD63 Antibody (AD1) [NBP2-68077] - Figure A. A647 conjugated mouse anti rat CD63 and FITC conjugated mouse IgG1 isotype control. Figure B. A647 conjugated mouse anti rat CD63 and FITC conjugated mouse anti rat CD61.](http://images.novusbio.com/fullsize/CD63-Antibody-AD1-Flow-Cytometry-NBP2-68077-img0004.jpg "Flow Cytometry: CD63 Antibody (AD1) [NBP2-68077] - Figure A. A647 conjugated mouse anti rat CD63 and FITC conjugated mouse IgG1 isotype control. Figure B. A647 conjugated mouse anti rat CD63 and FITC conjugated mouse anti rat CD61.")
![Flow Cytometry: CD63 Antibody (AD1) [NBP2-68077] - Rat peripheral blood platelets stained with Mouse anti Rat CD63 Alexa Fluor(R)488.](http://images.novusbio.com/fullsize/CD63-Antibody-AD1-Flow-Cytometry-NBP2-68077-img0001.jpg)
![Flow Cytometry: CD63 Antibody (AD1) [NBP2-68077] - Rat peritoneal macrophages stained with Mouse anti Rat CD63:Alexa Fluor(R)647.](http://images.novusbio.com/fullsize/CD63-Antibody-AD1-Flow-Cytometry-NBP2-68077-img0002.jpg)
![Flow Cytometry: CD63 Antibody (AD1) [NBP2-68077] - Rat peripheral blood platelets stained with Mouse anti Rat CD63:FITC.](http://images.novusbio.com/fullsize/CD63-Antibody-AD1-Flow-Cytometry-NBP2-68077-img0003.jpg)
![Western Blot: KIF3A Antibody [NBP3-05030] - Analysis of extracts of various cell lines, using KIF3A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/KIF3A-Antibody-Western-Blot-NBP3-05030-img0003.jpg "Western Blot: KIF3A Antibody [NBP3-05030] - Analysis of extracts of various cell lines, using KIF3A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")
![Immunocytochemistry/Immunofluorescence: KIF3A Antibody [NBP3-05030] - Analysis of MCF7 cells using KIF3A antibody . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/KIF3A-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05030-img0001.jpg)
![Immunoprecipitation: KIF3A Antibody [NBP3-05030] - Analysis of 200ug extracts of MCF-7 cells, using KIF3A antibody . Western blot was performed from the immunoprecipitate using this antibody.](http://images.novusbio.com/fullsize/KIF3A-Antibody-Immunoprecipitation-NBP3-05030-img0002.jpg)
![Immunohistochemistry-Paraffin: KIF3A Antibody [NBP3-05030] - Rat testis using KIF3A Rabbit pAb at dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/antibody/nbp3-05030_rabbit-polyclonal-kif3a-antibody-immunohistochemistry-paraffin-146202493146..jpg)
![Immunohistochemistry: CD21 Antibody [NBP3-38538] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using CD21 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38538_rabbit-cd21-pab-9122024159579.jpg "Immunohistochemistry: CD21 Antibody [NBP3-38538] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using CD21 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunocytochemistry/ Immunofluorescence: CD21 Antibody [NBP3-38538] - Immunofluorescence analysis of paraffin-embedded human spleen using CD21 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38538_rabbit-cd21-pab-912202415171172.jpg)
![Western Blot: CD21 Antibody [NBP3-38538] - Western blot analysis of various lysates using CD21 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-38538_rabbit-cd21-pab-912202414163018.jpg)
![Immunocytochemistry/ Immunofluorescence: CD21 Antibody [NBP3-38538] - Immunofluorescence analysis of paraffin-embedded mouse spleen using CD21 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38538_rabbit-cd21-pab-912202415121332.jpg)
![Immunocytochemistry/ Immunofluorescence: CD21 Antibody [NBP3-38538] - Immunofluorescence analysis of paraffin-embedded rat spleen using CD21 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38538_rabbit-cd21-pab-912202415171157.jpg)
![Western Blot: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis using CD33 mAb against HEK293 (1) and CD33 (AA: 15-237)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-2C6B7-Western-Blot-NBP2-37388-img0003.jpg "Western Blot: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis using CD33 mAb against HEK293 (1) and CD33 (AA: 15-237)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis of paraffin-embedded endometrium tissues using CD33 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-2C6B7-Immunohistochemistry-NBP2-37388-img0007.jpg)
![Flow Cytometry: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis of HepG2 cells using CD33 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-2C6B7-Flow-Cytometry-NBP2-37388-img0004.jpg)
![Western Blot: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis using CD33 mAb against human CD33 recombinant protein. (Expected MW is 49.2 kDa)](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-2C6B7-Western-Blot-NBP2-37388-img0002.jpg)
![Immunohistochemistry: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis of paraffin-embedded esophageal cancer tissues using CD33 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-2C6B7-Immunohistochemistry-NBP2-37388-img0005.jpg)
![ELISA: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-2C6B7-ELISA-NBP2-37388-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of Raji cells labeling CD86 with Rabbit anti-CD86 antibody at 1/500 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD86 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-67417_rabbit-monoclonal-b7-2-cd86-antibody-sj20-00-immunocytochemistry-immunofluorescence-2972024113947..jpg "Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of Raji cells labeling CD86 with Rabbit anti-CD86 antibody at 1/500 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD86 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,was used as the secondary antibody at 1/1,000 dilution.")
![Western Blot: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of CD86 on different lysates with Rabbit anti-CD86 antibody at 1/1,000 dilution.Lane 1: Raji cell lysate (10 ug/Lane)Lane 2: Mouse lung tissue lysate (20 ug/Lane)Lane 3: Rat lung tissue lysate (20 ug/Lane)Predicted band size: 38 kDaObserved band size: 70 kDaExposure time: 1 minute;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67417_rabbit-monoclonal-b7-2-cd86-antibody-sj20-00-western-blot-2972024114129..jpg)
![Western Blot: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of CD86 on different lysates with Rabbit anti-CD86 antibody at 1/2,000 dilution.Lane 1: Daudi cell lysateLane 2: Raji cell lysateLane 3: Ramos cell lysateLane 4: Jurkat cell lysateLane 5: K-562 cell lysateLysates/proteins at 15 ug/Lane.Predicted band size: 38 kDaObserved band size: 70 kDaExposure time: 2 minutes 37 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67417_rabbit-monoclonal-b7-2-cd86-antibody-sj20-00-western-blot-2972024114258..jpg)
![Western Blot: NEDD4 Antibody [NBP2-27197] - analysis of NEDD4 in mouse embryonic brain tissue lysate in the 1) absence and 2) presence of immunizing peptide using NEDD4 antibody at 5 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/NEDD4-Antibody-Western-Blot-NBP2-27197-img0001.jpg "Western Blot: NEDD4 Antibody [NBP2-27197] - analysis of NEDD4 in mouse embryonic brain tissue lysate in the 1) absence and 2) presence of immunizing peptide using NEDD4 antibody at 5 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: Insulin Antibody [NBP1-87485] - Staining in human pancreas and liver tissues using anti-INS antibody. Corresponding INS RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Insulin-Antibody-Immunohistochemistry-Paraffin-NBP1-87485-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Insulin Antibody [NBP1-87485] - Staining in human pancreas and liver tissues using anti-INS antibody. Corresponding INS RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Insulin Antibody [NBP1-87485] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/Insulin-Antibody-Immunohistochemistry-Paraffin-NBP1-87485-img0008.jpg)
![Immunohistochemistry-Paraffin: Insulin Antibody [NBP1-87485] - Staining of human pancreas shows high expression.](http://images.novusbio.com/fullsize/Insulin-Antibody-Immunohistochemistry-Paraffin-NBP1-87485-img0009.jpg)