| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86033-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87561-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18576-25ul | |||
| Azide and BSA Free | NBP3-44177 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85691-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Low Endotoxin, Azide and BSA Free | NBP2-75925SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47671-0.1mg | |||
| IHC-Prediluted | NBP2-48135 | |||
![Immunocytochemistry/Immunofluorescence: Amphiphysin/AMPH Antibody [NBP1-86033] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86033-img0005.jpg "Immunocytochemistry/Immunofluorescence: Amphiphysin/AMPH Antibody [NBP1-86033] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.")
![Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-86033] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-Paraffin-NBP1-86033-img0007.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/nbp1-86033_rabbit-polyclonal-amphiphysin-amph-antibody-24420259421711.jpg)
![Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-87561] - Staining in human cerebral cortex and tonsil tissues using anti-AMPH antibody. Corresponding AMPH RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-Paraffin-NBP1-87561-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-87561] - Staining in human cerebral cortex and tonsil tissues using anti-AMPH antibody. Corresponding AMPH RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-87561] - Staining of human cerebral cortex, colon, kidney and tonsil using Anti-AMPH antibody NBP1-87561 (A) shows similar protein distribution across tissues to independent antibody NBP1-86033 (B).](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-Paraffin-NBP1-87561-img0013.jpg)
![Western Blot: Amphiphysin/AMPH Antibody [NBP1-87561] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Western-Blot-NBP1-87561-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Amphiphysin/AMPH Antibody [NBP1-87561] - Staining of human cell line U-251 MG shows localization to plasma membrane and cytosol.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87561-img0012.jpg)
![Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-87561] - Staining of human kidney.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-Paraffin-NBP1-87561-img0016.jpg)
![Western Blot: Amphiphysin/AMPH Antibody [NBP1-87561] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Western-Blot-NBP1-87561-img0005.jpg)
![Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-87561] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-Paraffin-NBP1-87561-img0009.jpg)
![Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-87561] - Staining of human tonsil shows low expression as expected.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-Paraffin-NBP1-87561-img0010.jpg)
![Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-87561] - Staining of human colon.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-Paraffin-NBP1-87561-img0014.jpg)
![Western Blot: Melan-A/MART-1 Antibody (CL12863) [NBP3-18576] - Analysis in human cell line SK-MEL-30 and human cell line U-2 OS.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-CL12863-Western-Blot-NBP3-18576-img0007.jpg "Western Blot: Melan-A/MART-1 Antibody (CL12863) [NBP3-18576] - Analysis in human cell line SK-MEL-30 and human cell line U-2 OS.")
![Immunocytochemistry/Immunofluorescence: Melan-A/MART-1 Antibody (CL12863) [NBP3-18576] - Staining of SK-MEL-30 cells using the Anti-MART1 monoclonal antibody, showing specific staining in endoplasmic reticulum in green. Nuclear probes are visualized in blue (where available).](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-CL12863-Immunocytochemistry-Immunofluorescence-NBP3-18576-img0009.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (CL12863) [NBP3-18576] - Staining of human prostate shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-CL12863-Immunohistochemistry-Paraffin-NBP3-18576-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Melan-A/MART-1 Antibody (CL12863) [NBP3-18576] - Staining of SK-MEL-30 cells using the Anti-MART1 monoclonal antibody, showing specific staining in endoplasmic reticulum in green. Nuclear probes are visualized in blue (where available).](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-CL12863-Immunocytochemistry-Immunofluorescence-NBP3-18576-img0001.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (CL12863) [NBP3-18576] - Analysis in human skin and prostate tissues using NBP3-18576 antibody. Corresponding MLANA RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-CL12863-Immunohistochemistry-Paraffin-NBP3-18576-img0002.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (CL12863) [NBP3-18576] - Staining of human skin shows strong cytoplasmic positivity in melanocytes.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-CL12863-Immunohistochemistry-Paraffin-NBP3-18576-img0003.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (CL12863) [NBP3-18576] - Staining of human kidney shows no positivity as expected.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-CL12863-Immunohistochemistry-Paraffin-NBP3-18576-img0004.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (CL12863) [NBP3-18576] - Staining of human cervix shows no positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-CL12863-Immunohistochemistry-Paraffin-NBP3-18576-img0005.jpg)
![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Analysis in human liver and cerebral cortex tissues using NBP1-85691 antibody. Corresponding CES1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP1-85691-img0024.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Analysis in human liver and cerebral cortex tissues using NBP1-85691 antibody. Corresponding CES1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP1-85691-img0026.jpg)
![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Staining of human cerebral cortex shows no cytoplasmic positivity in neuronal cells as expected.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP1-85691-img0021.jpg)
![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Staining of human lung shows strong cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP1-85691-img0025.jpg)
![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Staining of human small intestine shows strong cytoplasmic positivity in enteroendocrine cells.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP1-85691-img0027.jpg)
![Simple Western: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Simple Western lane view shows a specific band for CES1 in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Simple-Western-NBP1-85691-img0006.jpg)
![Simple Western: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Electropherogram image(s) of corresponding Simple Western lane view. CES1 antibody was used at 1:20 dilution on RT-4 lysate(s).](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Simple-Western-NBP1-85691-img0007.jpg)
![Western Blot: TNF-alpha Antibody (2342C) - Low Endotoxin and Azide Free [NBP2-75925] - Western blot shows conditioned media from CHO Chinese hamster ovary cell line either mock transfected or transfected with human TNF- alpha. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human TNF-alpha Monoclonal Antibody (NBP2-75925) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for TNF-alpha at approximately 18 and 21 kDa (as indicated). This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-2342C-Low-Endotoxin-and-Azide-Free-Western-Blot-NBP2-75925-img0002.jpg "Western Blot: TNF-alpha Antibody (2342C) - Low Endotoxin and Azide Free [NBP2-75925] - Western blot shows conditioned media from CHO Chinese hamster ovary cell line either mock transfected or transfected with human TNF- alpha. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human TNF-alpha Monoclonal Antibody (NBP2-75925) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for TNF-alpha at approximately 18 and 21 kDa (as indicated). This experiment was conducted under reducing conditions.")
![Immunohistochemistry-Paraffin: TNF-alpha Antibody (2342C) - Low Endotoxin and Azide Free [NBP2-75925] - Analysis of a FFPE tissue section of the human intesting using 1:200 dilution of TNF-alpha antibody (NBP2-75925). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated a moderate to strong cytoplasmic staining in crypt cells.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-2342C-Low-Endotoxin-and-Azide-Free-Immunohistochemistry-Paraffin-NBP2-75925-img0003.jpg)
![Neutralization: TNF-alpha Antibody (2342C) [NBP2-75925] - Recombinant Human TNF-alpha (Catalog # 210-TA) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Cytotoxicity elicited by Recombinant Human TNF-alpha (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF-alpha Monoclonal Antibody (Catalog # NBP2-75925). The ND50 is typically 1-10 ng/mL in the presence of the metabolic inhibitor actinomycin D.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-2342C-Neutralization-NBP2-75925-img0001.jpg)
![Western Blot: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Whole cell lysate (10 µg) from HEK293T, HDLM-2, Jurkat,MJ, and HeLa cells prepared using NETN lysis buffer.Antibody: Rabbit anti-CD86 recombinant monoclonalantibody [BLR030F] used at 1:1000.Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with anexposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-western-blot-1311202411142..png "Western Blot: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Whole cell lysate (10 µg) from HEK293T, HDLM-2, Jurkat,MJ, and HeLa cells prepared using NETN lysis buffer.Antibody: Rabbit anti-CD86 recombinant monoclonalantibody [BLR030F] used at 1:1000.Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with anexposure time of 10 seconds.")
![Flow Cytometry: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Antibody: Rabbit anti-CD86 recombinantmonoclonal [BLR030F] or isotype control(unshaded). Secondary: DyLight® 488-conjugated goatanti-rabbit IgG.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-flow-cytometry-1311202411333..png)
![Immunohistochemistry-Paraffin: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] -FFPE section of tonsil. Antibody: Rabbit anti-CD86recombinant monoclonal antibody [BLR030F]. Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-immunohistochemistry-paraffin-131120241151..png)
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] -Section of HDLM-2 cells. Antibody: Rabbit anti-CD86recombinant monoclonal antibody [BLR030F]. Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-immunocytochemistry-immunofluorescence-1311202411712..png)
![Immunoprecipitation: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from HDLM-2 cells prepared using NETN lysis buffer. Antibodies: NBP2-76417 Lot 4 used for immunoprecipitation at 6 ul per reaction. B7-2/CD86 was also immunoprecipitated by a previous lot of this antibody (NBP2-76417 Lot 1) and a second antibody, NBP2-76416, against a different epitope of B7-2/CD86 (BLR029F). For blotting immunoprecipitated CD86, NBP2-76417 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-immunoprecipitation-1312025145029..jpg)
![Immunoprecipitation: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Detection of human CD86 by WB of immunoprecipitates from HDML-2 lysate. Antibodies: Rabbit anti-CD86 recombinant monoclonal [BLR030F] (NBP2-76417) and rabbit anti-CD86 recombinant monoclonal [BLR029F] (NBP2-76416).](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-immunoprecipitation-131202515750..jpg)
![Western Blot: MIF Antibody [NB100-1789] - Staining of Human Thymus lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/MIF-Antibody-Western-Blot-NB100-1789-img0005.jpg "Western Blot: MIF Antibody [NB100-1789] - Staining of Human Thymus lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry: MIF Antibody [NB100-1789] - Co-localization of CXCR4 and MIF in urothelium. Sections from rats treated with saline or CYP are shown. MIF immunostaining (green immunofluorescence), CXCR4 immunostaining (red immunofluorescence) and an overlay panel combining both immunostaining and a DAPI nuclear stain. MIF immunostaining is seen in basal and intermediate cells and in fibroblasts in the lamina propria of saline treated rats, while superficial cells do not stain for MIF. Arrows show luminal edge of urothelium. CXCR4 is restricted to basal and intermediate cells of urothelium. CYP treatment resulted in superficial cell staining for MIF and CXCR4 and overlay panels demonstrate co-localization as orange color in urothelial cells. Arrows point to superficial cells showing MIF-CXCR4 co-localization. Calibration bar=50 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0003898) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MIF-Antibody-Immunohistochemistry-NB100-1789-img0006.jpg)
![Western Blot: MIF Antibody [NB100-1789] - Staining of Daudi (A) and Jurkat (B) lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/MIF-Antibody-Western-Blot-NB100-1789-img0003.jpg)
![Immunohistochemistry-Paraffin: MIF Antibody [NB100-1789] - Staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/MIF-Antibody-Immunohistochemistry-Paraffin-NB100-1789-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: MIF Antibody [NB100-1789] - Co-localization of CXCR4 & MIF in urothelium.Representative sections from rats treated with saline (A–C) or CYP (D–I) are shown. The figure shows MIF immunostaining (green immunofluorescence), CXCR4 immunostaining (red immunofluorescence) & an overlay panel combining both immunostaining & a DAPI nuclear stain. MIF immunostaining is seen in basal & intermediate cells & in fibroblasts in the lamina propria of saline treated rats (A), while superficial cells do not stain for MIF. Arrows show luminal edge of urothelium. CXCR4 is restricted to basal & intermediate cells of urothelium (B) & lamina propria is not stained. Overlay of these panels (C) demonstrate co-localization of MIF & CXCR4 as orange coloring of cells. CYP treatment resulted in superficial cell staining for MIF (D,G) & CXCR4 (E,H) & overlay panels (F,I) demonstrate co-localization as orange color in urothelial cells. Arrows point to superficial cells showing MIF-CXCR4 co-localization. Calibration bar = 50 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/19066630), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1789_goat-polyclonal-mif-antibody-31020241554531.jpg)
![Immunohistochemistry-Paraffin: beta-2 Adrenergic R/ADRB2 Antibody (JM102-06) [NBP2-67187] - Analysis of paraffin-embedded mouse liver tissue using anti-ADRB2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/beta-2-Adrenergic-R-ADRB2-Antibody-JM102-06-Immunohistochemistry-Paraffin-NBP2-67187-img0002.jpg "Immunohistochemistry-Paraffin: beta-2 Adrenergic R/ADRB2 Antibody (JM102-06) [NBP2-67187] - Analysis of paraffin-embedded mouse liver tissue using anti-ADRB2 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: beta-2 Adrenergic R/ADRB2 Antibody (JM102-06) [NBP2-67187] - Analysis of paraffin-embedded mouse brain tissue using anti-ADRB2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/beta-2-Adrenergic-R-ADRB2-Antibody-JM102-06-Immunohistochemistry-Paraffin-NBP2-67187-img0001.jpg)
![Western Blot: beta-2 Adrenergic R/ADRB2 Antibody (JM102-06) [NBP2-67187] - Analysis of beta 2 Adrenergic Receptor on different lysates with Rabbit anti-beta 2 Adrenergic Receptor antibody at 1/5,000 dilution.Lane 1: A431 cell lysate (15 µg/Lane)Lane 2: Human liver tissue lysate (20 µg/Lane)Lane 3: Mouse heart tissue lysate (20 µg/Lane)Lane 4: Rat heart tissue lysate (20 µg/Lane)Lane 5: Mouse kidney tissue lysate (20 µg/Lane)Lane 6: Rat kidney tissue lysate (20 µg/Lane)Predicted band size: 46 kDaObserved band size: 55-100 kDaExposure time: 3 minutes; ECL: K1801;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67187_rabbit-monoclonal-beta-2-adrenergic-r-adrb2-antibody-jm102-06-western-blot-28620249199..jpg)
![Western Blot: beta-2 Adrenergic R/ADRB2 Antibody (JM102-06) [NBP2-67187] -Analysis of beta 2 Adrenergic Receptor on different lysates with Rabbit anti-beta 2 Adrenergic Receptor antibody at 1/1,000 dilution.Lane 1: A431 cell lysate (no heat) (15 µg/Lane)Lane 2: Mouse kidney tissue lysate (no heat) (20 µg/Lane)Lane 3: Mouse heart tissue lysate (no heat) (20 µg/Lane)Lane 4: Rat kidney tissue lysate (no heat) (20 µg/Lane)Lane 5: Zebrafish tissue lysate (20 µg/Lane)Predicted band size: 46 kDaObserved band size: 55-100 kDaExposure time: 3 minutes; ECL: K1801;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-04) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67187_rabbit-monoclonal-beta-2-adrenergic-r-adrb2-antibody-jm102-06-western-blot-286202492022..jpg)
![Flow Cytometry-beta-2 Adrenergic R/ADRB2 Antibody (JM102-06) [NBP2-67187] -Analysis of A431 cells labeling beta 2 Adrenergic Receptor.Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp2-67187_rabbit-monoclonal-beta-2-adrenergic-r-adrb2-antibody-jm102-06-flow-cytometry-28620249222..jpg)
![ICC/IF-beta-2 Adrenergic R/ADRB2 Antibody (JM102-06) [NBP2-67187] - Analysis of A431 cells labeling beta 2 Adrenergic Receptor with Rabbit anti-beta 2 Adrenergic Receptor antibody at 1/100 dilution.Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-beta 2 Adrenergic Receptor antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin ( red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-67187_rabbit-monoclonal-beta-2-adrenergic-r-adrb2-antibody-jm102-06-immunocytochemistry-immunofluorescence-286202492524..jpg)
![Western Blot: BDNF Antibody (SJ12-09) [NBP2-67410] - Analysis of BDNF on different lysates using anti-BDNF antibody at 1/1,000 dilution. Positive control: Lane 1: SHG-44 Lane 2: A172 Lane 3: Mouse brain](http://images.novusbio.com/fullsize/BDNF-Antibody-SJ12-09-Western-Blot-NBP2-67410-img0005.jpg "Western Blot: BDNF Antibody (SJ12-09) [NBP2-67410] - Analysis of BDNF on different lysates using anti-BDNF antibody at 1/1,000 dilution. Positive control: Lane 1: SHG-44 Lane 2: A172 Lane 3: Mouse brain")
![Immunocytochemistry/Immunofluorescence: BDNF Antibody (SJ12-09) [NBP2-67410] - ICC staining of BDNF in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary ant](http://images.novusbio.com/fullsize/BDNF-Antibody-SJ12-09-Immunocytochemistry-Immunofluorescence-NBP2-67410-img0006.jpg)
![Immunohistochemistry-Paraffin: BDNF Antibody (SJ12-09) [NBP2-67410] - Analysis of paraffin-embedded mouse testis tissue using anti-BDNF antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BDNF-Antibody-SJ12-09-Immunohistochemistry-Paraffin-NBP2-67410-img0004.jpg)
![Immunohistochemistry-Paraffin: BDNF Antibody (SJ12-09) [NBP2-67410] - Analysis of paraffin-embedded human lung tissue using anti-BDNF antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BDNF-Antibody-SJ12-09-Immunohistochemistry-Paraffin-NBP2-67410-img0001.jpg)
![Immunohistochemistry-Paraffin: BDNF Antibody (SJ12-09) [NBP2-67410] - Analysis of paraffin-embedded mouse brain tissue using anti-BDNF antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BDNF-Antibody-SJ12-09-Immunohistochemistry-Paraffin-NBP2-67410-img0002.jpg)
![Immunohistochemistry-Paraffin: BDNF Antibody (SJ12-09) [NBP2-67410] - Analysis of paraffin-embedded mouse heart tissue using anti-BDNF antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BDNF-Antibody-SJ12-09-Immunohistochemistry-Paraffin-NBP2-67410-img0003.jpg)
![Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-32505] - Paraffin-embedded mouse brain; antibody dilution 1:500.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-Paraffin-NBP1-32505-img0012.jpg "Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-32505] - Paraffin-embedded mouse brain; antibody dilution 1:500.")
![Immunohistochemistry: Amphiphysin/AMPH Antibody [NBP1-32505] - Paraffin-embedded U87 xenograft. Antibody at dilution of 1:500.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-NBP1-32505-img0009.jpg)
![Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-32505] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-Paraffin-NBP1-32505-img0010.jpg)
![Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-32505] - Paraffin-embedded RT2 xenograft; antibody dilution 1:500.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-Paraffin-NBP1-32505-img0011.jpg)
![Western Blot: Amphiphysin/AMPH Antibody [NBP1-32505] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Amphiphysin/AMPH antibody [N1N2], N-term (NBP1-32505) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32505_rabbit-polyclonal-amphiphysin-amph-antibody-91020242014696.jpg)
![Immunocytochemistry/ Immunofluorescence: Amphiphysin/AMPH Antibody [NBP1-32505] - Amphiphysin/AMPH antibody [N1N2], N-term detects Amphiphysin/AMPH protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Amphiphysin/AMPH stained by Amphiphysin/AMPH antibody [N1N2], N-term (NBP1-32505) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-32505_rabbit-polyclonal-amphiphysin-amph-antibody-9102024204124.jpg)
![Western Blot: Amphiphysin/AMPH Antibody [NBP1-32505] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Amphiphysin/AMPH antibody [N1N2], N-term (NBP1-32505) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32505_rabbit-polyclonal-amphiphysin-amph-antibody-91020242001078.jpg)
![Western Blot: IKK beta Antibody [NBP1-32258] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IKK beta antibody diluted at 1:500.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-Western-Blot-NBP1-32258-img0008.jpg "Western Blot: IKK beta Antibody [NBP1-32258] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IKK beta antibody diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: IKK beta Antibody [NBP1-32258] - analysis of A431, using IKK beta antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32258-img0002.jpg)
![Immunohistochemistry-Paraffin: IKK beta Antibody [NBP1-32258] - Paraffin-embedded Colon ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-32258-img0003.jpg)
![Western Blot: IKK beta Antibody [NBP1-32258] - IKK beta antibody detects IKK beta protein by western blot analysis. A. 30 ug 293T whole cell extract. B. 30 ug whole cell extract of DDDDK-human IKBKB-transfected 293T cells. 7.5 % SDS-PAGE. IKK beta antibody dilution: 1:5000](http://images.novusbio.com/fullsize/IKK-beta-Antibody-Western-Blot-NBP1-32258-img0006.jpg)
![Western Blot: IKK beta Antibody [NBP1-32258] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with IKK beta antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-Western-Blot-NBP1-32258-img0007.jpg)
![Western Blot: HLA DRB1 Antibody (HLA-DRB/1067) [NBP2-45314] - Western Blot Analysis of Ramos cell and human spleen tissue lysate using HLA DRB1 Antibody (HLA-DRB/1067).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-HLA-DRB-1067-Western-Blot-NBP2-45314-img0006.jpg "Western Blot: HLA DRB1 Antibody (HLA-DRB/1067) [NBP2-45314] - Western Blot Analysis of Ramos cell and human spleen tissue lysate using HLA DRB1 Antibody (HLA-DRB/1067).")
![Immunocytochemistry/Immunofluorescence: HLA DRB1 Antibody (HLA-DRB/1067) [NBP2-45314] - ICC/IF analysis of Raji cells labeling HLA-DR with conjugated with APC (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-HLA-DRB-1067-Immunofluorescence-NBP2-45314-img0005.jpg)
![Immunohistochemistry-Paraffin: HLA DRB1 Antibody (HLA-DRB/1067) [NBP2-45314] - Human Tonsil stained with HLA-DRB Monoclonal Antibody (HLA-DRB/1067).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-HLA-DRB-1067-Immunohistochemistry-Paraffin-NBP2-45314-img0002.jpg)
![Flow Cytometry: HLA DRB1 Antibody (HLA-DRB/1067) [NBP2-45314] - Flow Cytometric Analysis of human Raji cells using HLA DRB1 Antibody (HLA-DRB/1067).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-HLA-DRB-1067-Flow-Cytometry-NBP2-45314-img0004.jpg)
![Western Blot: HLA DRB1 Antibody (HLA-DRB/1067) [NBP2-45314] - Anaysis of Ramos Cell Lyste using HLA-DRB Monoclonal Antibody (HLA-DRB/1067).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-HLA-DRB-1067-Western-Blot-NBP2-45314-img0003.jpg)
![Immunohistochemistry-Paraffin: HLA DRB1 Antibody (HLA-DRB/1067) [NBP2-45314] - Human Histiocytoma stained with HLA-DRB Monoclonal Antibody (HLA-DRB/1067).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-HLA-DRB-1067-Immunohistochemistry-Paraffin-NBP2-45314-img0001.jpg)