| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-35040-1mg | |||
| Carrier-Free | NBP2-35040-100ug | |||
| Carrier-Free | NBP2-35040-500ug | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NBP1-75011 | |||
| Biotin | NBP1-75012 | |||
| DyLight 594 | NBP1-75888 | |||
| FITC | NBP1-75013 | |||
| HRP | NBP1-75014 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| FITC | NB500-622-100Tests | |||
| PE | NBP1-41282 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89364-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98752-50ul | |||
![SDS-Page: Human IL-17/IL-17A Protein [NBP2-35040]](http://images.novusbio.com/fullsize/Human-IL-17-IL-17A-Protein-SDS-Page-NBP2-35040-img0001.jpg "SDS-Page: Human IL-17/IL-17A Protein [NBP2-35040]")
![Western Blot: Siglec-3/CD33 Antibody (PSH02-51) [NBP3-32147] - Western blot analysis of Siglec-3/CD33 on different lysates with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32147) at 1/2,000 dilution. Lane 1: TF-1 cell lysate Lane 2: Jurkat cell lysate (negative) Lane 3: SK-MEL-28 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32147) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32147_rabbit-siglec-3-cd33-mab-psh02-51-278202415212472.jpg "Western Blot: Siglec-3/CD33 Antibody (PSH02-51) [NBP3-32147] - Western blot analysis of Siglec-3/CD33 on different lysates with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32147) at 1/2,000 dilution. Lane 1: TF-1 cell lysate Lane 2: Jurkat cell lysate (negative) Lane 3: SK-MEL-28 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32147) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Siglec-3/CD33 Antibody (PSH02-51) [NBP3-32147] - Immunocytochemistry analysis of TF-1 (positive) and Jurkat (negative) labeling Siglec-3/CD33 with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32147) at 1/500 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32147) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32147_rabbit-siglec-3-cd33-mab-psh02-51-29202410334035.jpg)
![Flow Cytometry: Siglec-3/CD33 Antibody (PSH02-51) [NBP3-32147] - Flow cytometric analysis of TF-1 cells labeling Siglec-3/CD33. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32147, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32147_rabbit-siglec-3-cd33-mab-psh02-51-29202416453237.jpg)
![Western Blot: TGF-beta 1 Antibody (PD00-17) [NBP3-33008] - Western blot analysis of TGF-beta 1 on different lysates with Rabbit anti-TGF-beta 1 antibody (NBP3-33008) at 1/2,000 dilution. Lane 1: Raji cell lysate (15 ug/Lane) Lane 2: K-562 cell lysate (15 ug/Lane) Lane 3: Mouse spleen tissue lysate (20 ug/Lane) Lane 4: Rat spleen tissue lysate (20 ug/Lane) Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 3 minutes 54 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33008) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33008_rabbit-tgf-beta-1-mab-pd00-17-288202412493428.jpg "Western Blot: TGF-beta 1 Antibody (PD00-17) [NBP3-33008] - Western blot analysis of TGF-beta 1 on different lysates with Rabbit anti-TGF-beta 1 antibody (NBP3-33008) at 1/2,000 dilution. Lane 1: Raji cell lysate (15 ug/Lane) Lane 2: K-562 cell lysate (15 ug/Lane) Lane 3: Mouse spleen tissue lysate (20 ug/Lane) Lane 4: Rat spleen tissue lysate (20 ug/Lane) Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 3 minutes 54 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33008) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: TGF-beta 1 Antibody (PD00-17) [NBP3-33008] - Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-TGF-beta 1 antibody (NBP3-33008) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33008) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33008_rabbit-tgf-beta-1-mab-pd00-17-29820241420920.jpg)
![Flow Cytometry: CD99 Antibody (MEM-131) [NB500-403] - Analysis using the FITC conjugate of NB500-403. Staining of Human peripheral blood lymphocytes with HCD99 FITC.](http://images.novusbio.com/fullsize/CD99-Antibody-MEM-131-Flow-Cytometry-NB500-403-img0004.jpg "Flow Cytometry: CD99 Antibody (MEM-131) [NB500-403] - Analysis using the FITC conjugate of NB500-403. Staining of Human peripheral blood lymphocytes with HCD99 FITC.")
![Flow Cytometry: CD99 Antibody (MEM-131) [NB500-403] - Analysis using the Biotin conjugate of NB500-403. Staining of Human peripheral blood lymphocytes biotinylated HCD99, followed by Sav-PE.](http://images.novusbio.com/fullsize/CD99-Antibody-MEM-131-Flow-Cytometry-NB500-403-img0003.jpg)
![Western Blot: Fc gamma RIIB/CD32b Antibody (110) [NBP2-89364] - Lane A: Daudi Whole Cell Lysate Lane B: THP1 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:34 kDa Observed band size:38 kDa](http://images.novusbio.com/fullsize/Fc-gamma-RIIB-CD32b-Antibody-110-Western-Blot-NBP2-89364-img0002.jpg "Western Blot: Fc gamma RIIB/CD32b Antibody (110) [NBP2-89364] - Lane A: Daudi Whole Cell Lysate Lane B: THP1 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:34 kDa Observed band size:38 kDa")
![Immunohistochemistry-Paraffin: Fc gamma RIIB/CD32b Antibody (110) [NBP2-89364] - Staining of human lymphonode showing membrane staining of B cells and some other mononuclear cell (1:10000).](http://images.novusbio.com/fullsize/Fc-gamma-RIIB-CD32b-Antibody-110-Immunohistochemistry-Paraffin-NBP2-89364-img0001.jpg)
![Western Blot: Stathmin 1 Antibody [NBP2-98752] - Anti-Stathmin 1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Rat brain tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 19 kDa. Observed band size: 19 kDa](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Western-Blot-NBP2-98752-img0001.jpg "Western Blot: Stathmin 1 Antibody [NBP2-98752] - Anti-Stathmin 1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Rat brain tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 19 kDa. Observed band size: 19 kDa")
![Immunohistochemistry-Paraffin: Stathmin 1 Antibody [NBP2-98752] - Immunochemical staining of human Stathmin 1 in human tonsil with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-98752-img0003.jpg)
![Immunohistochemistry-Paraffin: Stathmin 1 Antibody [NBP2-98752] - Immunochemical staining of human Stathmin 1 in human breast carcinoma with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-98752-img0002.jpg)
![Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (MR-1) [NBP1-47234] - Surface staining of activated murine splenocytes (PHA + PMA + ionomycin for 6 hours) using anti-CD154 (MR-1) FITC.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-MR-1-Flow-Cytometry-NBP1-47234-img0001.jpg "Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (MR-1) [NBP1-47234] - Surface staining of activated murine splenocytes (PHA + PMA + ionomycin for 6 hours) using anti-CD154 (MR-1) FITC.")
![Flow Cytometry: CD117/c-kit Antibody (104D2) [NBP3-09161] - (surface staining) of human peripheral blood cells with anti-CD117 (104D2) purified.](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-104D2-Flow-Cytometry-NBP3-09161-img0001.jpg "Flow Cytometry: CD117/c-kit Antibody (104D2) [NBP3-09161] - (surface staining) of human peripheral blood cells with anti-CD117 (104D2) purified.")
![Western Blot: HLA DR DP Antibody (MEM-136) [NB500-447] - Fig. 1. Western Blotting analysis of MHC Class II in whole cell lysate of RAJI human Burkitt lymphoma cell line using anti-human HLA-DR+DP (MEM-136). The antibody MEM-136 recognizes beta-chain of HLA-DR antigen under non-reducing conditions. Lane 1: immunostaining with anti-human HLA-DR+DP (MEM-136) Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06).](http://images.novusbio.com/fullsize/p_2392_1.jpg "Western Blot: HLA DR DP Antibody (MEM-136) [NB500-447] - Fig. 1. Western Blotting analysis of MHC Class II in whole cell lysate of RAJI human Burkitt lymphoma cell line using anti-human HLA-DR+DP (MEM-136). The antibody MEM-136 recognizes beta-chain of HLA-DR antigen under non-reducing conditions. Lane 1: immunostaining with anti-human HLA-DR+DP (MEM-136) Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06).")
![Western Blot: Siglec-3/CD33 Antibody (PSH02-50) [NBP3-32146] - Western blot analysis of Siglec-3/CD33 on different lysates with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32146) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: TF-1 cell lysate Lane 3: HL-60 cell lysate Lane 4: Jurkat cell lysate (negative) Lane 5: SK-MEL-28 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 70 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32146) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32146_rabbit-siglec-3-cd33-mab-psh02-50-278202415212431.jpg "Western Blot: Siglec-3/CD33 Antibody (PSH02-50) [NBP3-32146] - Western blot analysis of Siglec-3/CD33 on different lysates with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32146) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: TF-1 cell lysate Lane 3: HL-60 cell lysate Lane 4: Jurkat cell lysate (negative) Lane 5: SK-MEL-28 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 70 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32146) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Siglec-3/CD33 Antibody (PSH02-50) [NBP3-32146] - Immunocytochemistry analysis of TF-1 (positive) and Jurkat (negative) labeling Siglec-3/CD33 with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32146) at 1/200 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32146) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32146_rabbit-siglec-3-cd33-mab-psh02-50-29202410484839.jpg)
![Flow Cytometry: Siglec-3/CD33 Antibody (PSH02-50) [NBP3-32146] - Flow cytometric analysis of TF-1 cells labeling Siglec-3/CD33. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32146, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32146_rabbit-siglec-3-cd33-mab-psh02-50-29202416453211.jpg)
![Western Blot: CD11b Antibody (PSH03-96) [NBP3-32121] - Western blot analysis of CD11b on different lysates with Rabbit anti-CD11b antibody (NBP3-32121) at 1/2,000 dilution. Lane 1: TF-1 cell lysate (10 ug/Lane) Lane 2: THP-1 cell lysate (15 ug/Lane) Lane 3: U-937 cell lysate (30 ug/Lane) Lane 4: Jurkat cell lysate (negative) (10 ug/Lane) Lane 5: RAW264.7 cell lysate (5 ug/Lane) Lane 6: J774A.1 cell lysate (5 ug/Lane) Predicted band size: 127 kDa Observed band size: 170 kDa Exposure time: 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32121) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32121_rabbit-cd11b-mab-psh03-96-278202415295518.jpg "Western Blot: CD11b Antibody (PSH03-96) [NBP3-32121] - Western blot analysis of CD11b on different lysates with Rabbit anti-CD11b antibody (NBP3-32121) at 1/2,000 dilution. Lane 1: TF-1 cell lysate (10 ug/Lane) Lane 2: THP-1 cell lysate (15 ug/Lane) Lane 3: U-937 cell lysate (30 ug/Lane) Lane 4: Jurkat cell lysate (negative) (10 ug/Lane) Lane 5: RAW264.7 cell lysate (5 ug/Lane) Lane 6: J774A.1 cell lysate (5 ug/Lane) Predicted band size: 127 kDa Observed band size: 170 kDa Exposure time: 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32121) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD11b Antibody (PSH03-96) [NBP3-32121] - Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue with Rabbit anti-CD11b antibody (NBP3-32121) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32121) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32121_rabbit-cd11b-mab-psh03-96-29820241435252.jpg)