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![Immunohistochemistry-Paraffin: Tryptase alpha/TPS1 Antibody (AA1) [NBP2-26444] - Human tonsil stained with Mast Cell Tryptase antibody.](http://images.novusbio.com/fullsize/Tryptase-alpha-TPS1-Antibody-AA1-Immunohistochemistry-Paraffin-NBP2-26444-img0005.jpg "Immunohistochemistry-Paraffin: Tryptase alpha/TPS1 Antibody (AA1) [NBP2-26444] - Human tonsil stained with Mast Cell Tryptase antibody.")
![Immunohistochemistry-Paraffin: Tryptase alpha/TPS1 Antibody (AA1) [NBP2-26444] - Analysis of Mast Cell Tryptase formalin-fixed paraffin embedded human tonsil using NBP2-26444.](http://images.novusbio.com/fullsize/Tryptase-alpha-TPS1-Antibody-AA1-Immunohistochemistry-Paraffin-NBP2-26444-img0003.jpg)
![Immunohistochemistry-Paraffin: Mouse Monoclonal Tryptase alpha/beta 1 Antibody (AA1) [IMGENEX: IMG-80250] [NBP2-26444] - Analysis of Tryptase alpha/beta 1 in human liver tissue. Mast cell positive for Tryptase alpha/beta 1. pAb: x500 and DAPI counter stain. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-26444_mouse-monoclonal-tryptase-alpha-beta-1-antibody-aa1-imgenex-img-80250-immunohistochemistry-paraffin-722025161826..jpg)
![Western Blot: SHIP2/INPPL1 Antibody (3E6) [H00003636-M01] - INPPL1 monoclonal antibody (M01), clone 3E6 Analysis of INPPL1 expression in HeLa.](http://images.novusbio.com/fullsize/SHIP2-INPPL1-Antibody-3E6-Western-Blot-H00003636-M01-img0004.jpg "Western Blot: SHIP2/INPPL1 Antibody (3E6) [H00003636-M01] - INPPL1 monoclonal antibody (M01), clone 3E6 Analysis of INPPL1 expression in HeLa.")
![ELISA: SHIP2/INPPL1 Antibody (3E6) [H00003636-M01] - SHIP2/INPPL1 Antibody (3E6) [H00003636-M01] - Detection limit for recombinant GST tagged INPPL1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SHIP2-INPPL1-Antibody-3E6-ELISA-H00003636-M01-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: SYK Antibody [NBP1-32945] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32945-img0002.jpg "Immunocytochemistry/Immunofluorescence: SYK Antibody [NBP1-32945] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: SYK Antibody [NBP1-32945] - Paraffin-embedded rat spleen dilution: 1:500.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunohistochemistry-Paraffin-NBP1-32945-img0010.jpg)
![Western Blot: SYK Antibody [NBP1-32945] - Sample (30 ug of whole cell lysate) A: BMDM (bone marrow-derived macrophage) cell B: Syk null cell 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/SYK-Antibody-Western-Blot-NBP1-32945-img0004.jpg)
![Western Blot: SYK Antibody [NBP1-32945] - SYK antibody detects SYK protein by western blot analysis. Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SYK antibody at a dilution of 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SYK-Antibody-Western-Blot-NBP1-32945-img0011.jpg)
![Immunohistochemistry-Paraffin: SYK Antibody [NBP1-32945] - Paraffin-embedded human cervical cancer diluted at 1:500.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunohistochemistry-Paraffin-NBP1-32945-img0008.jpg)
![Immunohistochemistry-Paraffin: SYK Antibody [NBP1-32945] - Paraffin-embedded human endometrium diluted at 1:500.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunohistochemistry-Paraffin-NBP1-32945-img0009.jpg)
![Immunoprecipitation: SYK Antibody [NBP1-32945] - Immunoprecipitation of SYK protein from A431 whole cell extracts using 5 ug of SYK antibody. Western blot analysis was performed using SYK antibody.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunoprecipitation-NBP1-32945-img0013.jpg)
![Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Syk antibody [N2C2], Internal (NBP1-32945) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32945_rabbit-polyclonal-syk-antibody-western-blot-261120251820234.jpg)
![Immunohistochemistry-Paraffin: Histamine H4R Antibody [NLS3775] - Lung, Non Small-Cell Carcinoma](http://images.novusbio.com/fullsize/Histamine-H4R-Antibody-Immunohistochemistry-Paraffin-NLS3775-img0006.jpg "Immunohistochemistry-Paraffin: Histamine H4R Antibody [NLS3775] - Lung, Non Small-Cell Carcinoma")
![Immunohistochemistry-Paraffin: Histamine H4 R Antibody [NLS3775] - IHC analysis of Lymph node using this antibody at 12 ug/ ml.](http://images.novusbio.com/fullsize/Histamine-H4-R-Antibody-Immunohistochemistry-Paraffin-NLS3775-img0001.jpg)
![Immunohistochemistry-Paraffin: Histamine H4 R Antibody [NLS3775] - Anti-HRH4 / Histamine H4 Receptor antibody IHC of human Ovary, Carcinoma and Inflammatory Cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Histamine-H4-R-Antibody-Immunohistochemistry-Paraffin-NLS3775-img0002.jpg)
![Immunohistochemistry-Paraffin: Histamine H4R Antibody [NLS3775] - Ovary, carcinoma](http://images.novusbio.com/fullsize/Histamine-H4R-Antibody-Immunohistochemistry-Paraffin-NLS3775-img0003.jpg)
![Immunohistochemistry-Paraffin: Histamine H4R Antibody [NLS3775] - Pancreas, carcinoma](http://images.novusbio.com/fullsize/Histamine-H4R-Antibody-Immunohistochemistry-Paraffin-NLS3775-img0004.jpg)
![Immunohistochemistry-Paraffin: Histamine H4R Antibody [NLS3775] - Colon, Carcinoma](http://images.novusbio.com/fullsize/Histamine-H4R-Antibody-Immunohistochemistry-Paraffin-NLS3775-img0005.jpg)
![Immunohistochemistry-Paraffin: Histamine H4R Antibody - BSA Free [NLS3775] - Immunohistochemistry of formalin-fixed, paraffin-embedded Human Testis, Mast Cells tissue](http://images.novusbio.com/fullsize/nls3775_rabbit-histamine-h4r-pab-bsa-free-10920241111286.jpg)
![Western Blot: SHIP2/INPPL1 [p Tyr497] Antibody [NBP2-24461] - Analysis of phosphorylated SHIP2 (Y497) in recombinant fusion protein containing 1) a phosphorylated tyrosine at position 497 and 2) an unphosphorylated tyrosine at position 497, using SHIP2 phospho antibody at 0.01 ug/ml.](http://images.novusbio.com/fullsize/SHIP2-INPPL1-[p-Tyr497]-Antibody-Western-Blot-NBP2-24461-img0004.jpg "Western Blot: SHIP2/INPPL1 [p Tyr497] Antibody [NBP2-24461] - Analysis of phosphorylated SHIP2 (Y497) in recombinant fusion protein containing 1) a phosphorylated tyrosine at position 497 and 2) an unphosphorylated tyrosine at position 497, using SHIP2 phospho antibody at 0.01 ug/ml.")
![Immunohistochemistry-Paraffin: SHIP2/INPPL1 [p Tyr497] Antibody [NBP2-24461] - Analysis of phosphorylated SHIP2 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/SHIP2-INPPL1-[p-Tyr497]-Antibody-Immunohistochemistry-Paraffin-NBP2-24461-img0003.jpg)
![Immunohistochemistry-Paraffin: SYK Antibody [NBP1-86178] - Staining of human tonsil shows high expression.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunohistochemistry-Paraffin-NBP1-86178-img0007.jpg "Immunohistochemistry-Paraffin: SYK Antibody [NBP1-86178] - Staining of human tonsil shows high expression.")
![Immunohistochemistry-Paraffin: SYK Antibody [NBP1-86178] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunohistochemistry-Paraffin-NBP1-86178-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SYK Antibody [NBP1-86178] - Staining in human tonsil and liver tissues using anti-SYK antibody. Corresponding SYK RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunohistochemistry-Paraffin-NBP1-86178-img0008.jpg)
![Western Blot: Tryptase alpha/TPS1 Antibody (SC68-07) [NBP2-67608] - Human skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Tryptase-alpha-TPS1-Antibody-SC68-07-Western-Blot-NBP2-67608-img0001.jpg "Western Blot: Tryptase alpha/TPS1 Antibody (SC68-07) [NBP2-67608] - Human skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: Tryptase alpha/TPS1 Antibody (SC68-07) [NBP2-67608] - Human lung tissue using anti-Mast Cell Tryptase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Tryptase-alpha-TPS1-Antibody-SC68-07-Immunohistochemistry-Paraffin-NBP2-67608-img0005.jpg)
![Immunohistochemistry-Paraffin: Tryptase alpha/TPS1 Antibody (SC68-07) [NBP2-67608] - Human tonsil tissue using anti-Mast Cell Tryptase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Tryptase-alpha-TPS1-Antibody-SC68-07-Immunohistochemistry-Paraffin-NBP2-67608-img0002.jpg)
![Immunohistochemistry-Paraffin: Tryptase alpha/TPS1 Antibody (SC68-07) [NBP2-67608] - Human small intestine tissue using anti-Mast Cell Tryptase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Tryptase-alpha-TPS1-Antibody-SC68-07-Immunohistochemistry-Paraffin-NBP2-67608-img0003.jpg)
![Immunohistochemistry-Paraffin: Tryptase alpha/TPS1 Antibody (SC68-07) [NBP2-67608] - Human prostate carcinoma tissue using anti-Mast Cell Tryptase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Tryptase-alpha-TPS1-Antibody-SC68-07-Immunohistochemistry-Paraffin-NBP2-67608-img0004.jpg)
![Western Blot: SHIP2/INPPL1 Antibody [NB100-1592] - Detection of Human and Mouse INPPL2/SHIP2 by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from HeLa and (50 ug) from 293T and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-INPPL2/SHIP2 antibody NB100-1592 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/SHIP2-INPPL1-Antibody-Western-Blot-NB100-1592-img0011.jpg "<b>Biological Strategies Validation. </b>Western Blot: SHIP2/INPPL1 Antibody [NB100-1592] - Detection of Human and Mouse INPPL2/SHIP2 by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from HeLa and (50 ug) from 293T and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-INPPL2/SHIP2 antibody NB100-1592 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: SHIP2/INPPL1 Antibody [NB100-1592] - Sample: FFPE serial sections of human colon carcinoma. Antibody: Affinity purified rabbit anti-expndtw-6 INPPL1/SHIP2 Cat. No. Lot 1 (left) and Lot 2 (right) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SHIP2-INPPL1-Antibody-Immunohistochemistry-NB100-1592-img0009.jpg)
![Immunoprecipitation: SHIP2/INPPL1 Antibody [NB100-1592] - Detection of human INPPL2/SHIP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-INPPL2/SHIP2 antibody NB100-1592 (lot NB100-1592-2) used for IP at 6 ug per reaction. INPPL2/SHIP2 was also immunoprecipitated by a previous lot of this antibody (NB100-1592-1) and another rabbit anti-INPPL2/SHIP2 antibody. For blotting immunoprecipitated INPPL2/SHIP2, NB100-1592 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/SHIP2-INPPL1-Antibody-NB100-1592-img0012.jpg)
![Western Blot: ITCH/AIP4 Antibody [NB100-68142] - Staining of Human Brain (Frontal Cortex) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ITCH-AIP4-Antibody-Western-Blot-NB100-68142-img0002.jpg "Western Blot: ITCH/AIP4 Antibody [NB100-68142] - Staining of Human Brain (Frontal Cortex) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Flow Cytometry: ITCH/AIP4 Antibody [NB100-68142] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/ITCH-AIP4-Antibody-Flow-Cytometry-NB100-68142-img0005.jpg)
![Immunofluorescence: ITCH/AIP4 Antibody [NB100-68142] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/ITCH-AIP4-Antibody-Immunofluorescence-NB100-68142-img0003.jpg)
![Immunofluorescence: ITCH/AIP4 Antibody [NB100-68142] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/ITCH-AIP4-Antibody-Immunofluorescence-NB100-68142-img0004.jpg)
![Western Blot: SYK Antibody (SYK-01) [NBP3-09107] - Analysis (non-reducing conditions) of whole cell lysate of RAMOS human Burkitt lymphoma cell line (1), RBL rat basophilic leukemia cell line (2) and HeLa human cervix carcinoma cell line (3, 4). Lane 1, 2: immunostaining with anti-Syk (SYK-01) Lane 3, 4: immunostaining with anti-human Cytokeratin 18, Lane 5: negative control](http://images.novusbio.com/fullsize/SYK-Antibody-SYK-01-Western-Blot-NBP3-09107-img0001.jpg "Western Blot: SYK Antibody (SYK-01) [NBP3-09107] - Analysis (non-reducing conditions) of whole cell lysate of RAMOS human Burkitt lymphoma cell line (1), RBL rat basophilic leukemia cell line (2) and HeLa human cervix carcinoma cell line (3, 4). Lane 1, 2: immunostaining with anti-Syk (SYK-01) Lane 3, 4: immunostaining with anti-human Cytokeratin 18, Lane 5: negative control")
![Immunocytochemistry/Immunofluorescence: SYK Antibody (SYK-01) [NBP3-09107] - Analysis of Syk in human HeLa cell line using anti-Syk (SYK-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/SYK-Antibody-SYK-01-Immunocytochemistry-Immunofluorescence-NBP3-09107-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SYK Antibody (SYK-01) [NBP3-09107] - Analysis of Syk in human primary fibroblasts using anti-Syk (SYK-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/SYK-Antibody-SYK-01-Immunocytochemistry-Immunofluorescence-NBP3-09107-img0002.jpg)
![Western Blot: SYK Antibody (SYK-01) [NBP1-03250] - Lysates of THP-1 (WT and SYK KO) were prepared and 30 ug of protein were processed for immunoblot with SYK Antibody (SYK-01). The Ponceau stained transfer is shown. Antibody dilution used: 1/500. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/SYK-Antibody-SYK-01-Knockout-Validated-NBP1-03250-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: SYK Antibody (SYK-01) [NBP1-03250] - Lysates of THP-1 (WT and SYK KO) were prepared and 30 ug of protein were processed for immunoblot with SYK Antibody (SYK-01). The Ponceau stained transfer is shown. Antibody dilution used: 1/500. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).")
![<b>Genetic Strategies Validation. </b>Western Blot: SYK Antibody (SYK-01) [NBP1-03250] - Western blot of extracts from Human PBMC, SYK Antibody (SYK-01)](http://images.novusbio.com/fullsize/SYK-Antibody-SYK-01-Western-Blot-NBP1-03250-img0002.jpg)
![Immunohistochemistry-Paraffin: SYK Antibody (SYK-01) [NBP1-03250] - Staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/SYK-Antibody-SYK-01-Immunohistochemistry-Paraffin-NBP1-03250-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: SHIP2/INPPL1 Antibody [NBP1-87046] - Staining of human cell line U-251 MG shows localization to cytosol & the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SHIP2-INPPL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87046-img0004.jpg "Immunocytochemistry/Immunofluorescence: SHIP2/INPPL1 Antibody [NBP1-87046] - Staining of human cell line U-251 MG shows localization to cytosol & the Golgi apparatus. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: SHIP2/INPPL1 Antibody [NBP1-87046] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/fullsize/SHIP2-INPPL1-Antibody-Immunohistochemistry-Paraffin-NBP1-87046-img0002.jpg)