| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61715-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89951-50ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NB600-862-0.1mg | |||
| HRP | NB600-863-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1925SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB100-454SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-18644-0.02mg | |||
![Western Blot: HSP90 beta Antibody (5G4) [NBP2-37590] - Analysis using HSP90AB1 mouse mAb against Jurkat (1), A431 (2), Hela (3), A549 (4), HEK293 (5), K562 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-5G4-Western-Blot-NBP2-37590-img0002.jpg "Western Blot: HSP90 beta Antibody (5G4) [NBP2-37590] - Analysis using HSP90AB1 mouse mAb against Jurkat (1), A431 (2), Hela (3), A549 (4), HEK293 (5), K562 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.")
![Immunocytochemistry/Immunofluorescence: HSP90 beta Antibody (5G4) [NBP2-37590] - Analysis of Hela cells using HSP90AB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-5G4-Immunocytochemistry-Immunofluorescence-NBP2-37590-img0003.jpg)
![Immunohistochemistry-Paraffin: HSP90 beta Antibody (5G4) [NBP2-37590] - Analysis of paraffin-embedded kidney cancer tissues (left) and brain tissues (right) using HSP90AB1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-5G4-Immunohistochemistry-NBP2-37590-img0005.jpg)
![Flow Cytometry: HSP90 beta Antibody (5G4) [NBP2-37590] - Analysis of Hela cells using HSP90AB1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-5G4-Flow-Cytometry-NBP2-37590-img0004.jpg)
![ELISA: HSP90 beta Antibody (5G4) [NBP2-37590] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-5G4-ELISA-NBP2-37590-img0001.jpg)
![Western Blot: BNIP3 Antibody (6A5F7) [NBP2-61715] - Analysis using BNIP3 mAb against human BNIP3 (AA: 50-155) recombinant protein. (Expected MW is 38 kDa)](http://images.novusbio.com/fullsize/BNIP3-Antibody-6A5F7-Western-Blot-NBP2-61715-img0006.jpg "Western Blot: BNIP3 Antibody (6A5F7) [NBP2-61715] - Analysis using BNIP3 mAb against human BNIP3 (AA: 50-155) recombinant protein. (Expected MW is 38 kDa)")
![Immunocytochemistry/Immunofluorescence: BNIP3 Antibody (6A5F7) [NBP2-61715] - Analysis of Hela cells using BNIP3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/BNIP3-Antibody-6A5F7-Immunocytochemistry-Immunofluorescence-NBP2-61715-img0003.jpg)
![Immunohistochemistry-Paraffin: BNIP3 Antibody (6A5F7) [NBP2-61715] - Analysis of rectum cancer tissues using BNIP3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/BNIP3-Antibody-6A5F7-Immunohistochemistry-NBP2-61715-img0005.jpg)
![Flow Cytometry: BNIP3 Antibody (6A5F7) [NBP2-61715] - Analysis of Hela cells using BNIP3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/BNIP3-Antibody-6A5F7-Flow-Cytometry-NBP2-61715-img0002.jpg)
![Western Blot: BNIP3 Antibody (6A5F7) [NBP2-61715] - Analysis using BNIP3 mAb against HEK293 (1) and BNIP3 (AA: 50-155)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/BNIP3-Antibody-6A5F7-Western-Blot-NBP2-61715-img0007.jpg)
![Immunohistochemistry-Paraffin: BNIP3 Antibody (6A5F7) [NBP2-61715] - Analysis of breast cancer tissues using BNIP3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/BNIP3-Antibody-6A5F7-Immunohistochemistry-NBP2-61715-img0004.jpg)
![ELISA: BNIP3 Antibody (6A5F7) [NBP2-61715] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/BNIP3-Antibody-6A5F7-ELISA-NBP2-61715-img0001.jpg)
0.05, *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 compared to Control; +p < 0.05, ++p < 0.01, + + +p < 0.001 and + + + +p < 0.0001 compared to Cocl2; #p < 0.05, ##p < 0.01, ###p < 0.001, and ####p < 0.0001 compared to Hypoxia; ≠p < 0.05, ≠≠p < 0.01, ≠≠≠p < 0.001, and ≠≠≠≠p < 0.0001 compared to si-becn1 (21%O2) Vs, si-becn1 (1%O2). In western blots “→" represents a specific signal. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35928940), licensed under a CC-BY license. Not internally tested by Novus Biologicals." class="big_thumb" />
0.05, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 compared to Control; +p < 0.05, ++p < 0.01, + + +p < 0.001, and + + + +p < 0.0001 compared to Cocl2; #p < 0.05, ##p < 0.01, ###p < 0.001, and ####p < 0.0001 compared to Hypoxia; ≠p < 0.05, ≠≠p < 0.01, ≠≠≠p < 0.001, and ≠≠≠≠p < 0.0001 compared to si-becn1 (21%O2) Vs, si-becn1 (1%O2). In western blots “→" represents a specific signal. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35928940), licensed under a CC-BY license. Not internally tested by Novus Biologicals." class="big_thumb" />
0.05, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 compared to Control; +p < 0.05, ++p < 0.01, + + +p < 0.001, and + + + +p < 0.0001 compared to Cocl2; #p < 0.05, ##p < 0.01, ###p < 0.001, and ####p < 0.0001 compared to Hypoxia; ≠p < 0.05, ≠≠p < 0.01, ≠≠≠p < 0.001, and ≠≠≠≠p < 0.0001 compared to si-becn1 (21%O2) Vs, si-becn1 (1%O2). In western blots “→" represents a specific signal. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35928940), licensed under a CC-BY license. Not internally tested by Novus Biologicals." class="big_thumb" />
![Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032] - Western blot analysis of Pyruvate Dehydrogenase Kinase 1/PDK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: rat heart tissue lysate Lane 2: mouse heart tissue lysate](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-Kinase-1-PDK1-Antibody-JA67-30-Western-Blot-NBP2-67032-img0007.jpg "Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032] - Western blot analysis of Pyruvate Dehydrogenase Kinase 1/PDK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: rat heart tissue lysate Lane 2: mouse heart tissue lysate")
![Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032] - Staining PDK1 in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-Kinase-1-PDK1-Antibody-JA67-30-Immunocytochemistry-Immunofluorescence-NBP2-67032-img0005.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032] - Analysis of paraffin-embedded mouse heart tissue using anti-PDK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-Kinase-1-PDK1-Antibody-JA67-30-Immunohistochemistry-Paraffin-NBP2-67032-img0003.jpg)
![Flow Cytometry: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032] - Analysis of NIH-3T3 cells with PDK1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-Kinase-1-PDK1-Antibody-JA67-30-Flow-Cytometry-NBP2-67032-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032] - Staining PDK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-Kinase-1-PDK1-Antibody-JA67-30-Immunocytochemistry-Immunofluorescence-NBP2-67032-img0001.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032] - Analysis of paraffin-embedded human kidney tissue using anti-PDK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-Kinase-1-PDK1-Antibody-JA67-30-Immunohistochemistry-Paraffin-NBP2-67032-img0002.jpg)
![Western Blot: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Western blot analysis of CD40 Ligand/TNFSF5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse liver tissue lysate Lane 2: rat liver tissue lysate Lane 2: mouse bone marrow tissue lysate](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Western-Blot-NBP2-66756-img0007.jpg "Western Blot: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Western blot analysis of CD40 Ligand/TNFSF5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse liver tissue lysate Lane 2: rat liver tissue lysate Lane 2: mouse bone marrow tissue lysate")
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Staining CD40L in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunocytochemistry-Immunofluorescence-NBP2-66756-img0006.jpg)
![Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Analysis of paraffin-embedded human spleen tissue using anti- CD40L antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunohistochemistry-Paraffin-NBP2-66756-img0004.jpg)
![Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Analysis of THP-1 cells with CD40L antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Flow-Cytometry-NBP2-66756-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Staining CD40L in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunocytochemistry-Immunofluorescence-NBP2-66756-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Staining CD40L in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunocytochemistry-Immunofluorescence-NBP2-66756-img0003.jpg)
![Western Blot: HSP70/HSPA1A Antibody (001) [NBP2-89951] - Lane A: A549 Whole Cell LysateLane B: HCT116 Whole Cell LysateLane C: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:70 kDaObserved band size:70 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-001-Western-Blot-NBP2-89951-img0005.jpg "Western Blot: HSP70/HSPA1A Antibody (001) [NBP2-89951] - Lane A: A549 Whole Cell LysateLane B: HCT116 Whole Cell LysateLane C: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:70 kDaObserved band size:70 kDa(We are unsure as to the identity of these extra bands.)")
![Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (001) [NBP2-89951] - Staining of HSPA1A in cynomolgus macaque testis with rabbit monoclonal antibody (1:1000).](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89951-img0003.jpg)
![<b>Biological Strategies Validation. </b>Flow Cytometry: HSP70/HSPA1A Antibody (001) [NBP2-89951] - Analysis of Human HSPA1A expression on HeLa cells. The cells were treated, stained with purified anti-Human HSPA1A, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-001-Flow-Cytometry-NBP2-89951-img0004.jpg)
![Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (001) [NBP2-89951] - Staining of HSPA1A in human hepatoma with rabbit monoclonal antibody (1:1000).](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89951-img0002.jpg)
![Immunoprecipitation: HSP70/HSPA1A Antibody (001) [NBP2-89951] - Lane A:0.5 mg Hela Whole Cell Lysate. 2 uL anti-HSPA1A rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-HSPA1A rabbit monoclonal antibody,at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 70 kDa. Observed band size: 70 kDa](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-001-Immunoprecipitation-NBP2-89951-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody (001) [NBP2-89951] - Staining of Human HSPA1A in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89951 antibody (1:60) at 37? 1 hour. Then cells were stained with the Alexa Fluor 594-conjugated goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89951_rabbit-monoclonal-hsp70-hspa1a-antibody-001-235202521525031.png)
![Western Blot: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of DDIT3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Lane 1: LOVO cell lysate Lane 2: PC-12 cell lysate](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Western-Blot-NBP2-66856-img0007.jpg "Western Blot: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of DDIT3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Lane 1: LOVO cell lysate Lane 2: PC-12 cell lysate")
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded mouse pancreas tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0006.jpg)
![Flow Cytometry: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of Hela cells with DDIT3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Flow-Cytometry-NBP2-66856-img0001.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0002.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded human gastric carcinoma tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0003.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded human pancreas tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0004.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded mouse brain tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0005.jpg)
![Western blot analysis on different lysates with NBP2-66856 at 1/5,000 dilution. Lane 1: SW480 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Predicted band size: 19 kDa Observed band size: 27 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-66856 at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-66856_-western-blot-639204586669447398.jpg)
![Western blot analysis on different lysates with NBP2-66856 at 1/5,000 dilution. Lane 1: C2C12 cell lysate Lane 2: C2C12 treatment with 300 nM thapsigargin for 18h cell lysate Lane 3: C6 cell lysate Lane 4: C6 treatment with 300 nM thapsigargin for 18h cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: NBP2-66856, 1/5,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Goat Anti-Rabbit IgG [HRP] Secondary Antibody, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 19 kDa Observed band size: 27 kDa](http://images.novusbio.com/fullsize/nbp2-66856_-western-blot-639204598086981387.jpg)
![Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using HRP conjugate of NB600-861. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 ug per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH. Other band(s): None.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Western-Blot-NB600-861-img0012.jpg "Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using HRP conjugate of NB600-861. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 ug per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH. Other band(s): None.")
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase Antibody [NB600-861] - Analysis of HRP conjugate of NB600-861. Tissue: HeLa cells. Fixation: fixed for 5 min in 1:1 MeTOH:Acetone, blocked (preservative free) for 15 min. Antigen retrieval: not required. Primary antibody: Lactate Dehydrogenase antibody at 1:200 for](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Immunocytochemistry-Immunofluorescence-NB600-861-img0015.jpg)
![Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using Biotin conjugate of NB600-861. Lane 1: Lactate Dehydrogenase (Rabbit Muscle). Load: 50 ng per lane. Primary antibody: Goat anti-Lactate Dehydrogenase Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 36.5 kDa, 36.5 kDa for Lactate Dehydrogenase.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Western-Blot-NB600-861-img0009.jpg)
![Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using Biotin conjugate of NB600-861. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 ug per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH. Other band(s): None.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Western-Blot-NB600-861-img0010.jpg)
![Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using HRP conjugate of NB600-861. Lane 1: Lactate Dehydrogenase (Rabbit Muscle). Load: 50 ng per lane. Primary antibody: Goat anti-Lactate Dehydrogenase Antibody Peroxidase Conjugated at 1:1,000 overnight at 4C. Secondary antibody: n/a. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 36.5 kDa, 36.5 kDa for Lactate Dehydrogenase.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Western-Blot-NB600-861-img0011.jpg)
![Western Blot: JNK1 Antibody [NB100-1925] - Detection of JNK-1 using NB100-1925.](http://images.novusbio.com/fullsize/JNK1-Antibody-Western-Blot-NB100-1925-img0004.jpg "Western Blot: JNK1 Antibody [NB100-1925] - Detection of JNK-1 using NB100-1925.")
![Western Blot: PHD4/HIF Prolyl Hydroxylase 4 Antibody [NB100-295] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHD4 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/PHD4-HIF-Prolyl-Hydroxylase-4-Antibody-Western-Blot-NB100-295-img0007.jpg "Western Blot: PHD4/HIF Prolyl Hydroxylase 4 Antibody [NB100-295] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHD4 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: PHD4/HIF Prolyl Hydroxylase 4 Antibody [NB100-295] - Analysis of human PHD4, using NB100-295. Samples: whole cell lysate from HeLa cells, normoxic and hypoxic, respectively.](http://images.novusbio.com/fullsize/PHD4-HIF-Prolyl-Hydroxylase-4-Antibody-Western-Blot-NB100-295-img0004.jpg)
![Western Blot: PHD4/HIF Prolyl Hydroxylase 4 Antibody [NB100-295] - Whole cell lysate (25 mcg/lane) from human glioblastoma tumor cell lines or in vitro translated PHD4. Antibody: Affinity purified rabbit anti-PHD4 (Cat. No. NB100-295) used at 2 mcg/ml. Detection: Chemiluminescence](http://images.novusbio.com/fullsize/PHD4-HIF-Prolyl-Hydroxylase-4-Antibody-Western-Blot-NB100-295-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (LL48) [DDX0140P-100] - IF staining of hCD40L-transfected L cells with LL48 clone](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL48-Immunocytochemistry-Immunofluorescence-DDX0140P-100-img0005.jpg "Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (LL48) [DDX0140P-100] - IF staining of hCD40L-transfected L cells with LL48 clone")
![Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (LL48) [DDX0140P-100] - FACS staining of hCD40L-transfected L cells with LL48 clone](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL48-Flow-Cytometry-DDX0140P-100-img0002.jpg)
![Neutralization: CD40 Ligand/TNFSF5 Antibody (LL48) [DDX0140P-100] - Inhibition of IL12 production by CD40L-stimulated human monocyte-derived DCs by LL48 clone (Stax AM et al, 2008, J ImmunolMethods)](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL48-Neutralization-DDX0140P-100-img0003.jpg)
![Western Blot: VG5Q Antibody [NB100-454] - Human VG5Q detected in HT1080 non-transfected cell lysate (NTC) and HT1080 transfected cell lysate (TC) using NB 100-454.](http://images.novusbio.com/fullsize/VG5Q-Antibody-Western-Blot-NB100-454-img0003.jpg "Western Blot: VG5Q Antibody [NB100-454] - Human VG5Q detected in HT1080 non-transfected cell lysate (NTC) and HT1080 transfected cell lysate (TC) using NB 100-454.")
![Immunohistochemistry-Paraffin: VG5Q Antibody [NB100-454] - VG5Q was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human VG5Q polyclonal Antibody (Catalog # NB100-454) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in hepatocytes.](http://images.novusbio.com/fullsize/VG5Q-Antibody-Immunohistochemistry-Paraffin-NB100-454-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644] - VEGFR2/KDR/Flk-1 Antibody (4H3) [NBP1-18644] - IF double staining of human KDR in a co-culture of PAE-Flt-1, PAE-KDR and PAE-FLT-4 with (A) mouse anti-human KDR, (B) rabbit antihuman KDR (C) DAPI and (D) merged. Conjugated secondary antibody: goat anti-rabbit ALEXA Flour (1:600) and goat anti-mouse PE.](http://images.novusbio.com/fullsize/VEGFR2-KDR-Flk-1-Antibody-4H3-Immunocytochemistry-Immunofluorescence-NBP1-18644-img0017.jpg "Immunocytochemistry/Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644] - VEGFR2/KDR/Flk-1 Antibody (4H3) [NBP1-18644] - IF double staining of human KDR in a co-culture of PAE-Flt-1, PAE-KDR and PAE-FLT-4 with (A) mouse anti-human KDR, (B) rabbit antihuman KDR (C) DAPI and (D) merged. Conjugated secondary antibody: goat anti-rabbit ALEXA Flour (1:600) and goat anti-mouse PE.")
![Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (4H3) [NBP1-18644] - Analysis using the Biotin conjugate of NBP1-18644. Staining of VEGFR-2/KDR expression in HUVE cells.](http://images.novusbio.com/fullsize/VEGFR2-KDR-Flk-1-Antibody-4H3-Flow-Cytometry-NBP1-18644-img0011.jpg)
![Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (4H3) [NBP1-18644] - Fig. 1: Flow analysis of VEGF R2/KDR/Flk-1 expression in primary human dermal mivrovascular endothelial cells (HDMVEC) and human umbilical vein endothelial cells (HUVEC).](http://images.novusbio.com/fullsize/VEGFR2-KDR-Flk-1-Antibody-4H3-Flow-Cytometry-NBP1-18644-img0008.jpg)
![ELISA: VEGFR2/KDR/Flk-1 Antibody (4H3) [NBP1-18644] - 1 Sandwich-ELISA using soluble KDR (D7) as standard. Mouse anti-human VEGFR-2 was used as capture antibody, Biotinylated rabbit anti-human VEGFR-2 was used for detection.](http://images.novusbio.com/fullsize/VEGFR2-KDR-Flk-1-Antibody-4H3-ELISA-NBP1-18644-img0006.jpg)
![ELISA: VEGFR2/KDR/Flk-1 Antibody (4H3) [NBP1-18644] - Total lysate from PAE/KDR cells was measured for expression of VEGFR- 1/KDR by a standard Sandwich ELISA using a mouse monoclonal anti-human VEGFR-1 antibody for capturing and a Biotin conjugated rabbit polyclonal anti-human VEGFR-1 antibody for detection. Total lysate from PAE/Flt-1 and PAE/FLT-4 was used as negative control. As positive control, total lysate from PAE/Flt-1 was spiked with recombinant human sKDR.](http://images.novusbio.com/fullsize/VEGFR2-KDR-Flk-1-Antibody-4H3-ELISA-NBP1-18644-img0018.jpg)