| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-617SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47740-0.1mg | |||
| IHC-Prediluted | NBP2-48202 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP2-21678 | |||
| DyLight 488 | NBP1-77701 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43962 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43994 | |||
| BSA Free | NBP2-61631-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44433 | |||
| BSA Free | NBP2-42379-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08660 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43911 | |||
| BSA Free | NBP2-52952-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96887-0.025ml | |||
![Western Blot: KAT3B/p300 Antibody (RW109) [NB100-617] - p300 detected in a HeLa nuclear extract using NB100-617 (1:1,000). ECL: 20 minute exposure.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW109-Western-Blot-NB100-617-img0003.jpg "Western Blot: KAT3B/p300 Antibody (RW109) [NB100-617] - p300 detected in a HeLa nuclear extract using NB100-617 (1:1,000). ECL: 20 minute exposure.")
![Immunohistochemistry-Paraffin: EGFR Antibody (31G7) [NBP2-45148] - Human Placenta stained with EGFR Monoclonal Antibody (31G7).](http://images.novusbio.com/fullsize/EGFR-Antibody-31G7-Immunohistochemistry-Paraffin-NBP2-45148-img0001.jpg "Immunohistochemistry-Paraffin: EGFR Antibody (31G7) [NBP2-45148] - Human Placenta stained with EGFR Monoclonal Antibody (31G7).")
![Protein Array: EGFR Antibody (31G7) [NBP2-45148] - Analysis of Protein Array containing more than 19,000 full-length human proteins using EGFR Antibody (31G7) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/EGFR-Antibody-31G7-Protein-Array-NBP2-45148-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - A431 cells. Panel A: serum starved, unstimulated cells. Panel B: serum starved, EGF stimulated for 15 mins. A massive increase in AKT-pS473 activation, as measured by intensity signal, peaked at 15 minutes and was associated with depolymerized tubulin. Panel A shows STED data (AKT-pS473, red channel) collected simultaneously with confocal signal (alpha-tubulin, green channel). Upon stimulation of cells with EGF, a rapid activation of AKT is observed (Panel B) along with a coincident change in the tubulin organization (yellow signal), as well as an extensive cell shape-change (cell membrane folding) and accumulation of AKT pS473 at the cell periphery.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0011.jpg "Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - A431 cells. Panel A: serum starved, unstimulated cells. Panel B: serum starved, EGF stimulated for 15 mins. A massive increase in AKT-pS473 activation, as measured by intensity signal, peaked at 15 minutes and was associated with depolymerized tubulin. Panel A shows STED data (AKT-pS473, red channel) collected simultaneously with confocal signal (alpha-tubulin, green channel). Upon stimulation of cells with EGF, a rapid activation of AKT is observed (Panel B) along with a coincident change in the tubulin organization (yellow signal), as well as an extensive cell shape-change (cell membrane folding) and accumulation of AKT pS473 at the cell periphery.")
![Flow Cytometry: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis using the DyLight 488 conjugate of AKT1 phospho Ser473 antibody. Image shows anti-histone detection using a DyLight 488 conjugate (green). Anti-Tubulin was detected using a DyLight 549 conjugate (red). Nuclei were counter-stained using DAPI (blue).](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Flow-Cytometry-NBP1-69923-img0021.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT1 [p Ser473] antibody (17F6.B11). A: Lane 1) PDGF stimulated NIH 3T3 cells [10ul]. Lane 2) NIH 3T3 cells [10ul]. Lane 3) Hela whole cell lysate [10ul] (weak signal). B: Lane 4) GST negative control protein [100ng]. Lane 5) GST negative control protein [25ng]. Lane 6) AKT 1 recombinant protein [100ng]. Lane 7) AKT 1 recombinant protein [25ng].Block: 5% BSA overnight at 4C.Primary antibody: Monoclonal anti-AKT antibody used at 1:1000 for overnight at 4C.Secondary antibody: HRP Conjugated goat anti-mouse 1:25K for 45 min at RT.Detection: TMB for 20 minutes, rinsed with deionized water, dried and scanned on conventional flatbed scanner.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0045.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - High resolution STED immunofluorescence nanoscopy of AKT11 [p Ser473] antibody (17F6.B11). Tissue: A431 cells. The merge images (A) and at high magnification (B) show phosphorylated AKT1 colocalized with the distal microtubules. Fixation: 4% paraformaldehyde for 5 min and after washes blocked with 10% NGS/0.2% Triton X-100 for 30 min.Antigen retrieval: serum deprivation for 12 h.Primary antibody: AKT1 pS473 antibody at 10 ug/mL and alpha-tubulin (cyan) at 1.4 ug/mL for 1 h at RT.Secondary antibody: Atto 647N anti-Mouse IgG (ATTO TEC GmbH), and DyLight(TM)488 anti-Rabbit IgG were used at 1.0 ug/mL for 1h at RT for indirect detection.Localization: AKT1 pS473 is in the cytoplasm and also organized at the periphery of the cell.Staining: AKT1 pS473 as red signal with bis-benzimide (blue) nuclear counterstain.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0040.jpg)
![Immunohistochemistry: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Immunohistochemistry of AKT11 [p Ser473] antibody (17F6.B11). Tissue: human prostate tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: AKT1 pS473 antibody at 20 ug/mL for 1 h at RT.Secondary antibody: Dako's Techmate streptavidin-biotin reagents at 1:10,000 for 45 min at RT.Localization: AKT1 pS473 is nuclear and occasionally cytoplasmic.Staining: AKT1 pS473 as precipitated red signal with hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunohistochemistry-NBP1-69923-img0046.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT11 [p Ser473] antibody (17F6.B11). Lane 1: untreated NIH/3T3 cell lysates. Lane 2: detects phosphorylated AKT1 (indicated by arrowhead at ~56 kDa) on PDGF stimulated NIH/3T3 cell lysates. Load: 10 ug per lane.Primary antibody: AKT1 pS473 antibody at 1:10,000 in TBS with 0.05% Tween-20 with 1% BSA, for 1 h at 4 C.Secondary antibody: HRP conjugated Gt-a-Mouse IgG was used at a 1:20,000 dilution for 1 h at 4 C with FemtoMax(TM) enhanced chemiluminescent reagent.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0042.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT11 [p Ser473] antibody (17F6.B11). Lane 1: A431 cells. Lane 2: A431 cells stimulated for 15 min with EGF. Load: 35 ug per lane.Primary antibody: AKT1pS473 antibody at 1:400 for overnight at 4C.Secondary antibody: DyLight(TM)649 Conjugated Anti-AKT1 pS473 Monoclonal Antibody at 1:10,000 for 45 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting overnight at 4C.Predicted/Observed size: 56kDa. Other band(s): none.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0043.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT1 [p Ser473] antibody (17F6.B11). Lane 1: unstimulated NIH/3T3 lysates contain inactive unphosphorylated AKT11, green band. Lane 2: PDGF stimulated NIH/3T3 lysate contains both inactive (green band) and activated phosphorylated AKT11 (red band). Load: 10 ug per lane.Primary antibody: rabbit anti-AKT1 (pan) and mouse anti-AKT1 pS473 specific antibodies at 1:400 for overnight at 4C.Secondary antibody: DyLight(TM) 549 conjugated anti-rabbit IgG (green) and DyLight(TM) 649 conjugated anti-mouse IgG (red) secondary antibodies at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0044.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis of DyLight 488 conjugate of NBP1-69923. This image shows anti-histone detection using a DyLight 488 conjugate (green). Anti-Tubulin was detected using a DyLight 549 conjugate (red). Nuclei were counter-stained using DAPI (blue).](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0035.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923]](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0038.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Immunofluorescence Microscopy of AKT11 [p Ser473] antibody (17F6.B11) using STED nanoscopy to evaluate AKT1 activation and migration. Tissue: A431 cells. Antigen retrieval: Panel A: serum starved, unstimulated cells. Panel B: serum starved, EGF stimulated for 15 mins.A massive increase in AKT1-pS473 activation, as measured by intensity signal, peaked at 15 minutes and was associated with depolymerized tubulin.Staining: Panel A shows STED data (AKT1-pS473, red channel) collected simultaneously with confocal signal (a-tubulin, green channel). Upon stimulation of cells with EGF, a rapid activation of AKT1 is observed (Panel B) along with a coincident change in the tubulin organization (yellow signal), as well as an extensive cell shape-change (cell membrane folding) and accumulation of AKT1pS473 at the cell periphery.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0039.jpg)
![Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis of Biotin conjugate of NBP1-69923. 20 ug/mL for 1 h at RT Secondary antibody: Streptavidin Conj. HRP 10 ug/ml Localization: nuclear and occasionally cytoplasmic Staining: antibody as precipitated red signal with a hematoxylin purple nuclear count](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunohistochemistry-Paraffin-NBP1-69923-img0034.jpg)
![Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis of FFPE prostate tissue using AKT1 phospho Ser473 antibody (left panel). Negative control (right panel). Antigen retrieval with heat and pressure in citrate buffer pH 6.2. AKT1 phospho Ser473 antibody at 20 ug/mL. Secondary antibody Streptavidin-HRP at 10 ug/mL. Hematoxylin nuclear counterstain (purple). Image using the Biotin format of this antibody.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunohistochemistry-Paraffin-NBP1-69923-img0036.jpg)
![Western Blot of Mouse Anti-AKTpS473 antibody. Lane 1: A431 cells. Lane 2: A431 cells stimulated for 15 min with EGF. Load: 35 ug per lane. Primary antibody: AKTpS473 antibody at 1:400 for overnight at 4C. Secondary antibody: DyLight(TM)649 Conjugated Anti-AKT1 [p Ser473] Antibody (17F6.B11) at 1:10,000 for 45 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting overnight at 4C. Predicted/Observed size: 56kDa. Other band(s): none.](http://images.novusbio.com/fullsize/nbp1-69923_mouse-monoclonal-akt1-p-ser473-antibody-17f6-b11-255202313136.jpg)
![Western Blot: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Western blot analysis of VEGFR1/Flt-1 on mouse lung lysates using NBP3-11864 at 1/1,000 dilution.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-SY09-09-Western-Blot-NBP3-11864-img0001.jpg "Western Blot: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Western blot analysis of VEGFR1/Flt-1 on mouse lung lysates using NBP3-11864 at 1/1,000 dilution.")
![Immunocytochemistry/Immunofluorescence: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - ICC staining VEGFR1/Flt-1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-SY09-09-Immunocytochemistry-Immunofluorescence-NBP3-11864-img0006.jpg)
![Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using NBP3-11864. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-SY09-09-Immunohistochemistry-Paraffin-NBP3-11864-img0003.jpg)
![Flow Cytometry: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Flow cytometric analysis of A431 cells with NBP3-11864 at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-SY09-09-Flow-Cytometry-NBP3-11864-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Immunocytochemistry staining VEGFR1/Flt-1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-SY09-09-Immunocytochemistry-Immunofluorescence-NBP3-11864-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Immunocytochemistry staining VEGFR1/Flt-1 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-SY09-09-Immunocytochemistry-Immunofluorescence-NBP3-11864-img0005.jpg)
![Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using NBP3-11864. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-SY09-09-Immunohistochemistry-Paraffin-NBP3-11864-img0002.jpg)
![Western Blot: COX4 Antibody (CL3497) [NBP2-78718] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2, mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3497-Western-Blot-NBP2-78718-img0002.jpg "Western Blot: COX4 Antibody (CL3497) [NBP2-78718] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2, mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunohistochemistry-Paraffin: COX4 Antibody (CL3497) [NBP2-78718] - Staining of human prostate shows strong cytoplasmic immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3497-Immunohistochemistry-Paraffin-NBP2-78718-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: COX4 Antibody (CL3497) [NBP2-78718] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-COX4I1 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3497-Western-Blot-NBP2-78718-img0001.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (CL3497) [NBP2-78718] - Staining of human fallopian tube shows strong cytoplasmic positivity in epithelial cells.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3497-Immunohistochemistry-Paraffin-NBP2-78718-img0003.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (CL3497) [NBP2-78718] - Staining of human stomach shows strong cytoplasmic immunoreactivity in glandular epithelium cells.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3497-Immunohistochemistry-Paraffin-NBP2-78718-img0004.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (CL3497) [NBP2-78718] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3497-Immunohistochemistry-Paraffin-NBP2-78718-img0005.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (CL3497) [NBP2-78718] - Staining of human kidney shows strong cytoplasmic positivity in renal tubules and glomerulus cells.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3497-Immunohistochemistry-Paraffin-NBP2-78718-img0006.jpg)
![Immunohistochemistry-Paraffin: Snail Antibody (CL3700) [NBP2-61631] - Staining in human placenta and skeletal muscle tissues. Corresponding SNAI1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Snail-Antibody-CL3700-Immunohistochemistry-Paraffin-NBP2-61631-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Snail Antibody (CL3700) [NBP2-61631] - Staining in human placenta and skeletal muscle tissues. Corresponding SNAI1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Snail Antibody (CL3700) [NBP2-61631] - Staining of human colorectal cancer shows moderate to strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/Snail-Antibody-CL3700-Immunohistochemistry-Paraffin-NBP2-61631-img0001.jpg)
![Immunohistochemistry-Paraffin: Snail Antibody (CL3700) [NBP2-61631] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Snail-Antibody-CL3700-Immunohistochemistry-Paraffin-NBP2-61631-img0002.jpg)
![Immunohistochemistry-Paraffin: Snail Antibody (CL3700) [NBP2-61631] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Snail-Antibody-CL3700-Immunohistochemistry-Paraffin-NBP2-61631-img0003.jpg)
![Immunohistochemistry-Paraffin: Snail Antibody (CL3700) [NBP2-61631] - Staining of human skeletal muscle shows only weak nuclear positivity in striated muscle fibers.](http://images.novusbio.com/fullsize/Snail-Antibody-CL3700-Immunohistochemistry-Paraffin-NBP2-61631-img0004.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining in human prostate and skeletal muscle tissues using NBP2-42379 antibody. Corresponding HSP90B1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunohistochemistry-Paraffin-NBP2-42379-img0021.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining in human prostate and skeletal muscle tissues using NBP2-42379 antibody. Corresponding HSP90B1 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HSP90B1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Western-Blot-NBP2-42379-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining in U251 cell line showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunocytochemistry-Immunofluorescence-NBP2-42379-img0010.jpg)
![Western Blot: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431 and human cell line HepG2.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Western-Blot-NBP2-42379-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining in HeLa cell line showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunocytochemistry-Immunofluorescence-NBP2-42379-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining of human cell line A431 showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunocytochemistry-Immunofluorescence-NBP2-42379-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining in MCF7 cell line showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunocytochemistry-Immunofluorescence-NBP2-42379-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining in U2OS cell line with Anti-HSP90B1 monoclonal antibody, showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunocytochemistry-Immunofluorescence-NBP2-42379-img0009.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunohistochemistry-Paraffin-NBP2-42379-img0017.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunohistochemistry-Paraffin-NBP2-42379-img0018.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunohistochemistry-Paraffin-NBP2-42379-img0019.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining of human skeletal muscle shows very weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunohistochemistry-Paraffin-NBP2-42379-img0020.jpg)
![Immunohistochemistry-Paraffin: p63/TP73L Antibody (rTP40/3690) [NBP3-07430] - Formalin-fixed, paraffin-embedded human kidney stained with p63/TP73L Recombinant Mouse Monoclonal Antibody (rTP40/3690).](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-rTP40-3690-Immunohistochemistry-Paraffin-NBP3-07430-img0003.jpg "Immunohistochemistry-Paraffin: p63/TP73L Antibody (rTP40/3690) [NBP3-07430] - Formalin-fixed, paraffin-embedded human kidney stained with p63/TP73L Recombinant Mouse Monoclonal Antibody (rTP40/3690).")
![Flow Cytometry: p63/TP73L Antibody (rTP40/3690) [NBP3-07430] - Flow Cytometric Analysis of PFA-fixed HeLa cells using p63/TP73L Recombinant Mouse Monoclonal Antibody (rTP40/3690) followed by goat anti- Mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-rTP40-3690-Flow-Cytometry-NBP3-07430-img0001.jpg)
![Immunohistochemistry-Paraffin: p63/TP73L Antibody (rTP40/3690) [NBP3-07430] - Formalin-fixed, paraffin-embedded human skin stained with p63/TP73L Recombinant Mouse Monoclonal Antibody (rTP40/3690).](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-rTP40-3690-Immunohistochemistry-Paraffin-NBP3-07430-img0002.jpg)
![Western Blot: EGFR Antibody (CL0815) [NBP2-52952] - Lane 1: Marker [kDa], Lane 2: Human cell line A-431](http://images.novusbio.com/fullsize/EGFR-Antibody-CL0815-Western-Blot-NBP2-52952-img0004.jpg "Western Blot: EGFR Antibody (CL0815) [NBP2-52952] - Lane 1: Marker [kDa], Lane 2: Human cell line A-431")
![Immunohistochemistry-Paraffin: EGFR Antibody (CL0815) [NBP2-52952] - Staining of human ventricular cancer shows moderate membranous immunoreactivity in tumor cells.](http://images.novusbio.com/fullsize/EGFR-Antibody-CL0815-Immunohistochemistry-Paraffin-NBP2-52952-img0003.jpg)
![Immunohistochemistry-Paraffin: EGFR Antibody (CL0815) [NBP2-52952] - Staining of human placenta shows strong membranous positivity in trophoblast.](http://images.novusbio.com/fullsize/EGFR-Antibody-CL0815-Immunohistochemistry-Paraffin-NBP2-52952-img0001.jpg)
![Immunohistochemistry-Paraffin: EGFR Antibody (CL0815) [NBP2-52952] - Staining of human tonsil shows moderate membranous immunoreactivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/EGFR-Antibody-CL0815-Immunohistochemistry-Paraffin-NBP2-52952-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CHRND Antibody (88B) [NB120-2804] - Analysis of Nicotinic Acetylcholine Receptor using Anti-Nicotinic Acetylcholine Receptor Monoclonal Antibody (88B) shows staining in U251 Cells. Nicotinic Acetylcholine Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Nicotinic Acetylcholine Receptor at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/CHRND-Antibody-88B-Immunocytochemistry-Immunofluorescence-NB120-2804-img0006.jpg "Immunocytochemistry/Immunofluorescence: CHRND Antibody (88B) [NB120-2804] - Analysis of Nicotinic Acetylcholine Receptor using Anti-Nicotinic Acetylcholine Receptor Monoclonal Antibody (88B) shows staining in U251 Cells. Nicotinic Acetylcholine Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Nicotinic Acetylcholine Receptor at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.")
![Immunohistochemistry-Paraffin: CHRND Antibody (88B) [NB120-2804] - Normal biopsies of deparaffinized Mouse lymph node tissue.](http://images.novusbio.com/fullsize/CHRND-Antibody-88B-Immunohistochemistry-Paraffin-NB120-2804-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: CHRND Antibody (88B) [NB120-2804] - Analysis of Nicotinic Acetylcholine Receptor using Anti-Nicotinic Acetylcholine Receptor Monoclonal Antibody (88B) shows staining in Hela Cells. Nicotinic Acetylcholine Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Nicotinic Acetylcholine Receptor at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/CHRND-Antibody-88B-Immunocytochemistry-Immunofluorescence-NB120-2804-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: CHRND Antibody (88B) [NB120-2804] - Analysis of Nicotinic Acetylcholine Receptor using Anti-Nicotinic Acetylcholine Receptor Monoclonal Antibody (88B) shows staining in Neuro-2a Cells. Nicotinic Acetylcholine Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Nicotinic Acetylcholine Receptor at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/CHRND-Antibody-88B-Immunocytochemistry-Immunofluorescence-NB120-2804-img0005.jpg)
![Immunohistochemistry-Paraffin: CHRND Antibody (88B) [NB120-2804] - Normal biopsies of deparaffinized Mouse brain tissue.](http://images.novusbio.com/fullsize/CHRND-Antibody-88B-Immunohistochemistry-Paraffin-NB120-2804-img0001.jpg)
![Immunohistochemistry-Paraffin: CHRND Antibody (88B) [NB120-2804] - Normal biopsies of deparaffinized Mouse kidney tissue.](http://images.novusbio.com/fullsize/CHRND-Antibody-88B-Immunohistochemistry-Paraffin-NB120-2804-img0002.jpg)
![Western Blot: COX-1 Antibody [NB100-867] - Staining (1ug/ml) of U937 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/COX-1-Antibody-Western-Blot-NB100-867-img0010.jpg "Western Blot: COX-1 Antibody [NB100-867] - Staining (1ug/ml) of U937 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: COX-1 Antibody [NB100-867] - Staining (3.8µg/ml) of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-Staining.](http://images.novusbio.com/fullsize/COX-1-Antibody-Immunohistochemistry-Paraffin-NB100-867-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: COX-1 Antibody [NB100-867] - Intestinal tuft cells express advillin. (a) Immunohistochemistry of paraffin-embedded tissue from distal ileum of C57BL/6 J mice using advillin (green) & DCLK1 (red) antibodies. Nuclei are counter stained with DAPI. Both proteins co-localize in the same cell. Note that not all DCLK1 positive cells are positive for advillin expression. Right panel shows higher magnification of boxed area & shows co-localization of DCLK1 & advillin in a tuft cell. (b) Immunohistochemistry of paraffin embedded tissue from distal ileum of C57BL/6 J mice using advillin (green) & PTGS1 (red) antibodies. Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area & co-localization of PTGS1 & advillin in a tuft cell. (c) Immunohistochemistry of cryopreserved tissue from distal ileum of C57BL/6 J mice using Alexa Fluor 568 Phalloidin (red) & advillin (green). Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area & co-localization of advillin & F-actin at the apical surface of a tuft cell. Data shown are representative of n = 5 animals. Scale bar represents 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32483224), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-867_goat-polyclonal-cox-1-antibody-31020241628497.jpg)
![Immunocytochemistry/ Immunofluorescence: COX-1 Antibody [NB100-867] - In intestinal enteroids, tuft cells express advillin. (a) Immuno-histochemistry of enteroids from distal ileum of C57BL/6 J mice shows tuft cell hyperplasia 72 hours post IL-4 & IL-13 treatment. Control refers to untreated enteroids from C57BL/6 J mice. Advillin (green) & DCLK1 (red) co-localization was used to identify tuft cells. Nuclei are counter stained with DAPI. Right panel shows higher magnification of the boxed area. (b) Immunohistochemistry of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice, show co-localization in tuft cells of advillin (green) & DCLK1 (red) in the upper panel; & advillin & PTGS1 (red) in the lower panel. Nuclei were counter stained with DAPI. Right panels show higher magnification of boxed area. Data shown are representative of n = 5 animals. Scale bar represents 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32483224), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-867_goat-polyclonal-cox-1-antibody-31020241628492.jpg)
![Immunocytochemistry/ Immunofluorescence: COX-1 Antibody [NB100-867] - Tuft cells positive for advillin are negative for villin. (a) Immunohistochemistry & Virtual Channel confocal microscopy of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice, show co-localization of advillin (green), DCLK1 (red) & PTGS1 (magenta) within the same cells identified as tuft cells. Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area. (b) Immunohistochemistry of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice show co-localization in tuft cells of advillin (green) with F-actin (red) in the upper panel; & co-localization of advillin (red) & tubulin (green) in the lower panel. Nuclei were counter stained with DAPI. Right panel shows higher magnification of boxed area. Note the co-localization of advillin to the apical tuft that terminates near the perinuclear region of the cells. Advillin appears in vesiculated structures that co-localize with tubulin near the lateral cell surface & in the cytoplasm. (c) Immunohistochemistry of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice show the absence of villin (red) from advillin (green) expressing tuft cells. Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area. Data shown are representative of n = 5 animals. Scale bar represents 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32483224), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-867_goat-polyclonal-cox-1-antibody-310202416284923.jpg)
![Western Blot: ERK1/2 Antibody [NB110-96887] - Western blot analysis of Human Cell line lysates showing detection of ERK1/2 protein using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Western-Blot-NB110-96887-img0013.jpg "Western Blot: ERK1/2 Antibody [NB110-96887] - Western blot analysis of Human Cell line lysates showing detection of ERK1/2 protein using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody [NB110-96887] - Analysis using Rabbit Anti-ERK1 Polyclonal Antibody. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Cytoplasm. Nucleus.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-96887-img0011.jpg)
![Immunohistochemistry: ERK1/2 Antibody [NB110-96887] - Immunohistochemistry analysis using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-NB110-96887-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody [NB110-96887] - Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Erk1/2 Polyclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Erk1/2 Antibody. (C) Composite.Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Erk1/2 Antibody. (C) Composite.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-96887-img0009.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody [NB110-96887] - Bouin's fixed paraffin embedded backskin sections of transgenic mice. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-Paraffin-NB110-96887-img0003.jpg)
![Immunohistochemistry: ERK1/2 Antibody [NB110-96887] - Immunohistochemistry analysis using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Tissue: Inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:25000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-NB110-96887-img0014.jpg)