| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70834 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76648SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71528 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37300-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Unpurified | NB100-185SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75978SS | |||
| BSA Free | NBP2-75978 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76810SS | |||
| BSA Free | NBP2-76810 | |||
![Western Blot: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR expression in transfected 293T cell line by AHR monoclonal antibody (M02), clone 3B12.Lane 1: AHR transfected lysate(96.147 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/AHR-Antibody-3B12-Western-Blot-H00000196-M02-img0016.jpg "Western Blot: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR expression in transfected 293T cell line by AHR monoclonal antibody (M02), clone 3B12.Lane 1: AHR transfected lysate(96.147 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: AHR Antibody (3B12) [H00000196-M02] - Analysis of monoclonal antibody to AHR on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/AHR-Antibody-3B12-Immunocytochemistry-Immunofluorescence-H00000196-M02-img0012.jpg)
![Western Blot: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR expression in COLO 320 HSR ( Cat # L020V1 ).](http://images.novusbio.com/fullsize/AHR-Antibody-3B12-Western-Blot-H00000196-M02-img0008.jpg)
![Western Blot: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR over-expressed 293 cell line, cotransfected with AHR Validated Chimera RNAi ( Cat # H00000196-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with AHR monoclonal antibody (M02), clone 3B12 (Cat # H00000196-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/AHR-Antibody-3B12-Western-Blot-H00000196-M02-img0014.jpg)
![Immunoprecipitation: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR transfected lysate using anti-AHR monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with AHR MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/AHR-Antibody-3B12-Immunoprecipitation-H00000196-M02-img0013.jpg)
![ELISA: AHR Antibody (3B12) [H00000196-M02] - Detection limit for recombinant GST tagged AHR is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/AHR-Antibody-3B12-ELISA-H00000196-M02-img0015.jpg)
![Western Blot: Glutamine Synthetase Antibody (1F4) [NBP2-02125] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Glutamine Synthetase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Western-Blot-NBP2-02125-img0009.jpg "Western Blot: Glutamine Synthetase Antibody (1F4) [NBP2-02125] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Glutamine Synthetase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: Glutamine Synthetase Antibody (OTI1F4) [NBP2-02125] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Glutamine Synthetase.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Immunocytochemistry-Immunofluorescence-NBP2-02125-img0012.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - Staining of paraffin-embedded Human Ovary tissue using anti-Glutamine Synthetase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Immunohistochemistry-Paraffin-NBP2-02125-img0015.jpg)
![Flow Cytometry: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - Analysis of Jurkat cells, using anti-Glutamine Synthetase antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Flow-Cytometry-NBP2-02125-img0011.jpg)
![Western Blot: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Glutamine Synthetase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Glutamine Synthetase.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Western-Blot-NBP2-02125-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - Immunofluorescent staining of HepG2 cells using anti-Glutamine Synthetase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Immunocytochemistry-Immunofluorescence-NBP2-02125-img0004.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - Staining of paraffin-embedded Human breast tissue using anti-Glutamine Synthetase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Immunohistochemistry-Paraffin-NBP2-02125-img0013.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - Staining of paraffin-embedded Human endometrium tissue using anti-Glutamine Synthetase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Immunohistochemistry-Paraffin-NBP2-02125-img0014.jpg)
![Flow Cytometry: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - Analysis of Hela cells, using anti-Glutamine Synthetase antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Flow-Cytometry-NBP2-02125-img0010.jpg)
![Immunohistochemistry: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation of Beclin 1 in Rat Spleen Tissue. Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-Beclin 1 antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76648_rabbit-polyclonal-beclin-1-antibody-2711202420175183.gif "Immunohistochemistry: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation of Beclin 1 in Rat Spleen Tissue. Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-Beclin 1 antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
![Immunohistochemistry: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation of Beclin 1 in Rat Liver Tissue. Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Beclin 1 antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76648_rabbit-polyclonal-beclin-1-antibody-2711202419261860.gif)
![Immunohistochemistry: Beclin 1 Antibody [NBP1-76648] - Beclin-1 in human brain tissue with Beclin-1 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Immunohistochemistry-NBP1-76648-img0004.jpg)
![Immunohistochemistry: Beclin 1 Antibody [NBP1-76648] - Beclin-1 in mouse brain tissue with Beclin-1 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Immunohistochemistry-NBP1-76648-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation of Beclin 1 in Mouse Brain Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain cells labeling Beclin 1 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).](http://images.novusbio.com/fullsize/nbp1-76648_rabbit-polyclonal-beclin-1-antibody-2711202419451274.gif)
![Immunohistochemistry: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation of Beclin 1 in Mouse Kidney Tissue.Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Beclin 1 antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76648_rabbit-polyclonal-beclin-1-antibody-271120241958491.gif)
![Immunocytochemistry/ Immunofluorescence: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation of Beclin 1 in HELA Cells.Immunofluorescent analysis of 4% paraformaldehyde-fixed Hela cells labeling Beclin 1 at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).](http://images.novusbio.com/fullsize/nbp1-76648_rabbit-polyclonal-beclin-1-antibody-2711202419251598.gif)
![Western Blot: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation in Human Cell Lines.Loading: 15 ug of lysate Antibodies: Beclin 1, 1 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76648_rabbit-polyclonal-beclin-1-antibody-271120242016947.gif)
![Western Blot: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation in Mouse Cell Lines.Loading: 15 ug of lysate Antibodies: Beclin 1, 1 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76648_rabbit-polyclonal-beclin-1-antibody-2711202420115589.gif)
![Western Blot: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation in Rat Cell Lines.Loading: 15 ug of lysate Antibodies: Beclin 1, 1 ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76648_rabbit-polyclonal-beclin-1-antibody-2711202419592041.gif)
![Western Blot: AHR Antibody (RPT1) [NB300-530] - Analysis of 20ug of HepG2, U2OS, and K562 whole cell lysates and 5uL of PageRuler Plus Prestained Protein Ladder per well.](http://images.novusbio.com/fullsize/AHR-Antibody-RPT1-Western-Blot-NB300-530-img0013.jpg "Western Blot: AHR Antibody (RPT1) [NB300-530] - Analysis of 20ug of HepG2, U2OS, and K562 whole cell lysates and 5uL of PageRuler Plus Prestained Protein Ladder per well.")
![Immunocytochemistry/Immunofluorescence: AHR Antibody (RPT1) [NB300-530] - Analysis of Aryl Hydrocarbon Receptor in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Aryl Hydrocarbon Receptor monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Aryl Hydrocarbon Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/AHR-Antibody-RPT1-Immunocytochemistry-Immunofluorescence-NB300-530-img0010.jpg)
![Immunohistochemistry-Paraffin: AHR Antibody (RPT1) [NB300-530] - Cancer biopsies of deparaffinized Human breast carcinoma tissue at a dilution of 1:100.](http://images.novusbio.com/fullsize/AHR-Antibody-RPT1-Immunohistochemistry-Paraffin-NB300-530-img0012.jpg)
![Western Blot: AHR Antibody (RPT1) [NB300-530] - Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an Aryl Hydrocarbon Receptor monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:20,000 for 1 hour.](http://images.novusbio.com/fullsize/AHR-Antibody-RPT1-Western-Blot-NB300-530-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: AHR Antibody (RPT1) [NB300-530] - Analysis of Aryl Hydrocarbon Receptor in NIH-3T3 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Aryl Hydrocarbon Receptor monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Aryl Hydrocarbon Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/AHR-Antibody-RPT1-Immunocytochemistry-Immunofluorescence-NB300-530-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: AHR Antibody (RPT1) [NB300-530] - Analysis of Aryl Hydrocarbon Receptor in MCF-7 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Aryl Hydrocarbon Receptor monoclonal antibody at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Aryl Hydrocarbon Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/AHR-Antibody-RPT1-Immunocytochemistry-Immunofluorescence-NB300-530-img0009.jpg)
![Immunohistochemistry-Paraffin: AHR Antibody (RPT1) [NB300-530] - Normal biopsies of deparaffinized Human skeletal muscle tissue at a dilution of 1:20.](http://images.novusbio.com/fullsize/AHR-Antibody-RPT1-Immunohistochemistry-Paraffin-NB300-530-img0011.jpg)
![Western Blot: AKT3 Antibody (OTI9B2) [NBP1-47657] - Analysis of extracts (10ug) from 2 different cell lines by using anti-AKT3 monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/AKT3-Antibody-OTI9B2-Western-Blot-NBP1-47657-img0006.jpg "Western Blot: AKT3 Antibody (OTI9B2) [NBP1-47657] - Analysis of extracts (10ug) from 2 different cell lines by using anti-AKT3 monoclonal antibody at 1:200 dilution.")
![Immunocytochemistry/Immunofluorescence: AKT3 Antibody (9B2) [NBP1-47657] Staining of COS7 cells transiently transfected by pCMV6-ENTRY AKT3.](http://images.novusbio.com/fullsize/AKT3-Antibody-9B2-Immunocytochemistry-Immunofluorescence-NBP1-47657-img0005.jpg)
![Immunohistochemistry-Paraffin: AKT3 Antibody (9B2) [NBP1-47657] - Staining of paraffin-embedded Human lung tissue using anti-AKT3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AKT3-Antibody-9B2-Immunohistochemistry-Paraffin-NBP1-47657-img0004.jpg)
![Flow Cytometry: AKT3 Antibody (9B2) [NBP1-47657] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AKT3 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/AKT3-Antibody-9B2-Flow-Cytometry-NBP1-47657-img0002.jpg)
![Western Blot: AKT3 Antibody (9B2) [NBP1-47657] - AKT3 antibody ( 9B2 ) at 1:500 dilution + BALB/C 3T3 cell lysate](http://images.novusbio.com/fullsize/AKT3-Antibody-9B2-Western-Blot-NBP1-47657-img0001.jpg)
![Western Blot: AKT3 Antibody (9B2) [NBP1-47657] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT3.](http://images.novusbio.com/fullsize/AKT3-Antibody-9B2-Western-Blot-NBP1-47657-img0003.jpg)
![Western Blot: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis using PLIN2 mouse mAb against HepG2 cell lysate.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Western-Blot-NBP2-37300-img0004.jpg "Western Blot: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis using PLIN2 mouse mAb against HepG2 cell lysate.")
![Immunocytochemistry/Immunofluorescence: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Immunofluorescence analysis of HepG2 cells using PLIN2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Immunocytochemistry-Immunofluorescence-NBP2-37300-img0005.jpg)
![Immunohistochemistry-Paraffin: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis of bladder cancer tissues using PLIN2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Immunohistochemistry-NBP2-37300-img0008.jpg)
![Flow Cytometry: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis of HepG2 cells using PLIN2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Flow-Cytometry-NBP2-37300-img0006.jpg)
![Western Blot: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis using PLIN2 mAb against human PLIN2 (AA: 286-437) recombinant protein. (Expected MW is 42.6 kDa)](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Western-Blot-NBP2-37300-img0002.jpg)
![Western Blot: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis using PLIN2 mAb against HEK293 (1) and PLIN2 (AA: 286-437)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Western-Blot-NBP2-37300-img0003.jpg)
![Immunohistochemistry-Paraffin: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis of rectum cancer tissues using PLIN2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Immunohistochemistry-NBP2-37300-img0007.jpg)
![ELISA: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-ELISA-NBP2-37300-img0001.jpg)
![Western Blot: PARP2 Antibody [NB100-185] - Detection of PARP2 in NIH/3T3 cell lysate with polyclonal PARP2 antibody at a dilution of 1:250](http://images.novusbio.com/fullsize/PARP2-Antibody-Western-Blot-NB100-185-img0001.jpg "Western Blot: PARP2 Antibody [NB100-185] - Detection of PARP2 in NIH/3T3 cell lysate with polyclonal PARP2 antibody at a dilution of 1:250")
![Western Blot: PARP2 Antibody [NB100-185] - Western blot of analysis of PARP2 in 3T3 cell lysate using NB100-185 at 1:250 dilution.](http://images.novusbio.com/fullsize/PARP2-Antibody-Western-Blot-NB100-185-img0002.jpg)
![Western Blot: PARP2 Antibody [NB100-185] - Activation of signaling pathways by dandelion root extract. Western blots of proteins involved in programmed cell death and cell survival and inflammation. The protein levels, including ULK1 (NBP2-24738), Bcl-2 related protein A1 (NBP1-76715), and PARP2 (NB100-185) corresponded to the mRNA levels obtained during the gene expression analysis. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27564258/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nb100-185_rabbit-polyclonal-parp2-antibody-unpurified-25520231539510.jpg)
![Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of GSK-3 alpha/beta [p Tyr216, p Tyr279] on mouse brain tissue lysates with Rabbit anti-GSK-3 alpha/beta [p Tyr216, p Tyr279] antibody at 1:500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 46/51 kDa Observed band size: 46/51 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1:500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Western-Blot-NBP2-67441-img0013.jpg "Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of GSK-3 alpha/beta [p Tyr216, p Tyr279] on mouse brain tissue lysates with Rabbit anti-GSK-3 alpha/beta [p Tyr216, p Tyr279] antibody at 1:500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 46/51 kDa Observed band size: 46/51 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1:500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Staining Phospho-GSK3(alpha+beta)(Y216+Y279) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunocytochemistry-Immunofluorescence-NBP2-67441-img0003.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded human thyroid carcinoma tissue with Rabbit anti-Phospho-GSK3 alpha/beta [p Tyr216, p Tyr279) antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0017.jpg)
![Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of Phospho-GSK3(alpha+beta)(Y216+Y279) on mouse cerebellum tissue lysates. Lane 1: mouse cerebellum tissue, whole cell lysate, 20ug/laneLane 2: mouse cerebellum tissue treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 20ug/lane All lanes : Anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 47/51 kDaObserved band size: 47/51 kDaBlocking and diluting buffer: 5% BSA.Exposure time: 2 minutes](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Western-Blot-NBP2-67441-img0010.jpg)
![Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of Phospho-GSK3(alpha+beta)(Y216+Y279) on 293T cell lysates.Lane 1: 293T cells, whole cell lysate, 10ug/lane Lane 2: 293T cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes :Anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 47/51 kDaObserved band size: 47/51 kDaBlocking and diluting buffer: 5% BSA.Exposure time: 2 minutes](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Western-Blot-NBP2-67441-img0011.jpg)
![Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of Phospho-GSK3(alpha+beta)(Y216+Y279) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: A431 cell lysate Lane 2: Hela cell lysate](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Western-Blot-NBP2-67441-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Staining Phospho-GSK3(alpha+beta)(Y216+Y279) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunocytochemistry-Immunofluorescence-NBP2-67441-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Staining Phospho-GSK3(alpha+beta)(Y216+Y279) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunocytochemistry-Immunofluorescence-NBP2-67441-img0002.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0004.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded mouse brain tissue using anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0005.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded rat brain tissue using anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0006.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded rat brain tissue with Rabbit anti-Phospho-GSK-3 alpha/beta (p Tyr216+Y279) antibody000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0014.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-GSK-3 alpha/beta [p Tyr216, p Tyr279) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0015.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-GSK3 alpha/beta [p Tyr216, p Tyr279] antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Myosin heavy chain 11 Antibody (JA03-35) [NBP2-66967] - Staining smooth muscle Myosin heavy chain 11 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-JA03-35-Immunocytochemistry-Immunofluorescence-NBP2-66967-img0002.jpg "Immunocytochemistry/Immunofluorescence: Myosin heavy chain 11 Antibody (JA03-35) [NBP2-66967] - Staining smooth muscle Myosin heavy chain 11 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (JA03-35) [NBP2-66967] - Analysis of paraffin-embedded rat trachea tissue using anti-smooth muscle Myosin heavy chain 11 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-JA03-35-Immunohistochemistry-Paraffin-NBP2-66967-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Myosin heavy chain 11 Antibody (JA03-35) [NBP2-66967] - Staining smooth muscle Myosin heavy chain 11 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-JA03-35-Immunocytochemistry-Immunofluorescence-NBP2-66967-img0001.jpg)
![Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (JA03-35) [NBP2-66967] - Analysis of paraffin-embedded human colon tissue using anti-smooth muscle Myosin heavy chain 11 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-JA03-35-Immunohistochemistry-Paraffin-NBP2-66967-img0003.jpg)
![Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (JA03-35) [NBP2-66967] - Analysis of paraffin-embedded human jejunum tissue using anti-smooth muscle Myosin heavy chain 11 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-JA03-35-Immunohistochemistry-Paraffin-NBP2-66967-img0004.jpg)
![Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (JA03-35) [NBP2-66967] - Analysis of paraffin-embedded human prostate tissue using anti-smooth muscle Myosin heavy chain 11 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-JA03-35-Immunohistochemistry-Paraffin-NBP2-66967-img0005.jpg)
![Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (JA03-35) [NBP2-66967] - Analysis of paraffin-embedded human stomach cancer tissue using anti-smooth muscle Myosin heavy chain 11 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-JA03-35-Immunohistochemistry-Paraffin-NBP2-66967-img0006.jpg)
![Western Blot: ORP150/HSP12A Antibody [NBP1-32140] - Mouse tissue extracts (50 ug) was separated by 5% SDS-PAGE, and blotted with ORP150 antibodydiluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-Western-Blot-NBP1-32140-img0011.jpg "Western Blot: ORP150/HSP12A Antibody [NBP1-32140] - Mouse tissue extracts (50 ug) was separated by 5% SDS-PAGE, and blotted with ORP150 antibodydiluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: ORP150/HSP12A Antibody [NBP1-32140] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ORP150 protein stained by ORP150 antibody [C2C3], C-term diluted at 1:200. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32140-img0015.jpg)
![Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody [NBP1-32140] - Meta-analysis of significant metastatic upregulated proteins in acid exosomes through Prognoscan. Confocal immunofluorescence analysis with the indicated antibodies on primary (apical and mid region), and metastatic melanoma biopsies from the same patient. Ki67 labeling was used as internal control. Red, CFL, GSN, HYOU1; blue, DAPI; green, Ki67. Bar 50 um Image collected and cropped by CiteAb from the following publication (//jeccr.biomedcentral.com/articles/10.1186/s13046-018-0915-z), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-Immunohistochemistry-Paraffin-NBP1-32140-img0016.jpg)
![Western Blot: ORP150/HSP12A Antibody [NBP1-32140] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and blotted with ORP150 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-Western-Blot-NBP1-32140-img0008.jpg)
![Western Blot: ORP150/HSP12A Antibody [NBP1-32140] - Rat tissue extracts (50 ug) was separated by 5% SDS-PAGE, and blotted with ORP150 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-Western-Blot-NBP1-32140-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: ORP150/HSP12A Antibody [NBP1-32140] - Analysis of methanol-fixed Hep3B, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32140-img0005.jpg)
![Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody [NBP1-32140] - Paraffin-embedded CL1-0 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-Immunohistochemistry-Paraffin-NBP1-32140-img0006.jpg)
![Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody [NBP1-32140] - Paraffin-embedded mouse fore brain. ORP150 antibody [C2C3], C-term diluted at 1:500.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-Immunohistochemistry-Paraffin-NBP1-32140-img0007.jpg)
![Immunocytochemistry/ Immunofluorescence: ORP150/HSP12A Antibody [NBP1-32140] - Meta-analysis of significant metastatic upregulated proteins in acid exosomes through Prognoscan. a Kaplan-Meyer curves depicting the Overall Survival of metastatic melanoma patients with high (red lines) or low (green lines) expression of the genes listed in Table 1 that resulted statistically significant. Mentel-Cox p-values for each plot are showed. b Western blotting analysis of ctr & pH 6.0 exosomes (20 μg). Cell lysate was used as positive control. Expression of CFL, GSN & HSP90 alpha beta is shown. Ponceau red is shown for equal exosome protein amount. c confocal immunofluorescence analysis with the indicated antibodies on primary (apical & mid region), & metastatic melanoma biopsies from the same patient. Ki67 labeling was used as internal control. Red, CFL, GSN, HYOU1; blue, DAPI; green, Ki67. Bar 50 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30290833), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32140_rabbit-polyclonal-orp150-hsp12a-antibody-31020241615469.jpg)
![ORP150/HSP12A antibody [C2C3], C-term detects ORP150/HSP12A protein at endoplasmic reticulum by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ORP150/HSP12A protein stained by ORP150/HSP12A antibody [C2C3], C-term (NBP1-32140) diluted at 1:200.Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-32140_rabbit-polyclonal-orp150-hsp12a-antibody-immunocytochemistry-immunofluorescence-141120251841517.jpg)
![ORP150/HSP12A antibody [C2C3], C-term detects ORP150/HSP12A protein at cytoplasm by immunofluorescent analysis.Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ORP150/HSP12A protein stained by ORP150/HSP12A antibody [C2C3], C-term (NBP1-32140) diluted at 1:1000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-32140_rabbit-polyclonal-orp150-hsp12a-antibody-immunocytochemistry-immunofluorescence-141120251839103.jpg)
![Western Blot: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Western-Blot-NBP2-80765-img0001.jpg "Western Blot: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80765-img0002.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - HIF-1 alpha (clone 2443C) was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) at 1.0 ug/mL for 1 hour at room temperature followed by incubation with the](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80765-img0003.jpg)
![Simple Western: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20 ug/mL. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Simple-Western-NBP2-80765-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at approximately 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Knockout-Validated-NBP2-80765-img0004.jpg)
![Western Blot: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - Total protein from human HeLa, THP-1 and U87 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-PHD2 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Western-Blot-NBP2-80713-img0001.jpg "Western Blot: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - Total protein from human HeLa, THP-1 and U87 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-PHD2 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - EGLN1/PHD2 was detected in immersion fixed WM-115 human malignant melanoma cell line using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog #NBP2-76810) at 3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm and nuclei. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80713-img0002.jpg)
![Immunohistochemistry-Paraffin: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - EGLN1/PHD2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-76810) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte(TM).](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80713-img0003.jpg)
![Flow Cytometry: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - Human Jurkat T Cell Leukemia Cell Line was stained with Rabbit Anti- EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-76810, filled histogram) or Rabbit IgG Isotype Control Antibody (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80713-img0004.jpg)