| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB2416-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Unpurified | NB100-185SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70834 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71528 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76648SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37300-0.025ml | |||
![Western Blot: Rictor Antibody (1F3) [H00253260-M01] - RICTOR monoclonal antibody (M01), clone 1F3 Analysis of RICTOR expression in Hela S3 NE.](http://images.novusbio.com/fullsize/Rictor-Antibody-1F3-Western-Blot-H00253260-M01-img0011.jpg "Western Blot: Rictor Antibody (1F3) [H00253260-M01] - RICTOR monoclonal antibody (M01), clone 1F3 Analysis of RICTOR expression in Hela S3 NE.")
![Immunohistochemistry-Paraffin: Rictor Antibody (1F3) [H00253260-M01] - Analysis of monoclonal antibody to RICTOR on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Rictor-Antibody-1F3-Immunohistochemistry-Paraffin-H00253260-M01-img0008.jpg)
![Western Blot: Rictor Antibody (1F3) [H00253260-M01] - Analysis of RICTOR expression in transfected 293T cell line by RICTOR monoclonal antibody (M01), clone 1F3.Lane 1: RICTOR transfected lysate(29.8 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Rictor-Antibody-1F3-Western-Blot-H00253260-M01-img0012.jpg)
![Immunoprecipitation: Rictor Antibody (1F3) [H00253260-M01] - Analysis of RICTOR transfected lysate using anti-RICTOR monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RICTOR MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Rictor-Antibody-1F3-Immunoprecipitation-H00253260-M01-img0009.jpg)
![Sandwich ELISA: Rictor Antibody (1F3) [H00253260-M01] - Detection limit for recombinant GST tagged RICTOR is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Rictor-Antibody-1F3-Sandwich-ELISA-H00253260-M01-img0010.jpg)
![Western Blot: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR expression in transfected 293T cell line by AHR monoclonal antibody (M02), clone 3B12.Lane 1: AHR transfected lysate(96.147 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/AHR-Antibody-3B12-Western-Blot-H00000196-M02-img0016.jpg "Western Blot: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR expression in transfected 293T cell line by AHR monoclonal antibody (M02), clone 3B12.Lane 1: AHR transfected lysate(96.147 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: AHR Antibody (3B12) [H00000196-M02] - Analysis of monoclonal antibody to AHR on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/AHR-Antibody-3B12-Immunocytochemistry-Immunofluorescence-H00000196-M02-img0012.jpg)
![Western Blot: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR expression in COLO 320 HSR ( Cat # L020V1 ).](http://images.novusbio.com/fullsize/AHR-Antibody-3B12-Western-Blot-H00000196-M02-img0008.jpg)
![Western Blot: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR over-expressed 293 cell line, cotransfected with AHR Validated Chimera RNAi ( Cat # H00000196-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with AHR monoclonal antibody (M02), clone 3B12 (Cat # H00000196-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/AHR-Antibody-3B12-Western-Blot-H00000196-M02-img0014.jpg)
![Immunoprecipitation: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR transfected lysate using anti-AHR monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with AHR MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/AHR-Antibody-3B12-Immunoprecipitation-H00000196-M02-img0013.jpg)
![ELISA: AHR Antibody (3B12) [H00000196-M02] - Detection limit for recombinant GST tagged AHR is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/AHR-Antibody-3B12-ELISA-H00000196-M02-img0015.jpg)
![Western Blot: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of CD34 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: TF-1 cell lysate Lane 2: Mouse lung tissue lysate](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Western-Blot-NBP2-67399-img0007.jpg "Western Blot: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of CD34 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: TF-1 cell lysate Lane 2: Mouse lung tissue lysate")
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - (G) CD34 + microvascular staining in EOC tissue. Correlation between HE4 serum levels and CD34 + area in patient EOC tissue (n = 14). **p < 0.005, ****p < 0.0005. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-020-65353-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0008.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded rat kidney tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0003.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0004.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded mouse kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0006.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human tonsil tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0005.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human tonsil tissue labeling CD34 with Rabbit anti-CD34 antibody washed with PBS, and then probed with the primary antibody green) at 1/50 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0009.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0010.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human placenta tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0011.jpg)
![Western Blot: nNOS Antibody (60718) [MAB2416] - Analysis of mouse and rat brain Tissue lysate with ug/ml of hnNOS Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. A specific band was detected for hnNOS at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.](http://images.novusbio.com/fullsize/nNOS-Antibody-60718-Western-Blot-MAB2416-img0001.jpg "Western Blot: nNOS Antibody (60718) [MAB2416] - Analysis of mouse and rat brain Tissue lysate with ug/ml of hnNOS Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. A specific band was detected for hnNOS at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.")
![Western Blot: nNOS Antibody (60718) [MAB2416] - Effects of IFN alpha and IFN gamma treatment on nNOS expression levels in human metastatic melanoma A375 cells. Cells were treated with IFN alpha or IFN gamma for various timepoints and whole cell lysates were collected. Samples were subjected to Western blot analysis for nNOS. A control of the protein loading was performed by detecting actin. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35105915/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/mab2416_mouse-monoclonal-nnos-antibody-60718-2552023153952.jpg)
![Western Blot: COX4 Antibody (854) [NBP2-43540] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (854)diluted at 1:1000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/COX4-Antibody-854-Western-Blot-NBP2-43540-img0012.jpg "Western Blot: COX4 Antibody (854) [NBP2-43540] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (854)diluted at 1:1000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![Western Blot: COX4 Antibody (854) [NBP2-43540] - Analysis of A. 50 ug mouse heart lysate/extract 12 % SDS-PAGE COX4 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/COX4-Antibody-854-Western-Blot-NBP2-43540-img0003.jpg)
![Western Blot: COX4 Antibody (854) [NBP2-43540] - Analysis of A. 50 ug rat muscle lysate/extract 15 % SDS-PAGE COX4 antibody [854] dilution: 1:1000.](http://images.novusbio.com/fullsize/COX4-Antibody-854-Western-Blot-NBP2-43540-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: COX4 Antibody (854) [NBP2-43540] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [854]. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/COX4-Antibody-854-Western-Blot-NBP2-43540-img0007.jpg)
![Western Blot: COX4 Antibody (854) [NBP2-43540] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (854) diluted at 1:1000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/COX4-Antibody-854-Western-Blot-NBP2-43540-img0011.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (854) [NBP2-43540] - Human ovarian cancer. COX4 stained by COX4 antibody [854] diluted at 1:200.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min.](http://images.novusbio.com/fullsize/COX4-Antibody-854-Immunohistochemistry-Paraffin-NBP2-43540-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: COX4 Antibody (854) [NBP2-43540] - COX4 antibody detects COX4 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: COX4 stained by COX4 antibody (NBP2-43540) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-43540_mouse-monoclonal-cox4-antibody-854-910202420134064.jpg)
![COX4 antibody [GT854] detects COX4 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded human colon cancer.COX4 stained by COX4 antibody [GT854] (NBP2-43540) diluted at 1:200.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43540_mouse-monoclonal-cox4-antibody-854-immunohistochemistry-paraffin-2611202518243910.jpg)
![Western Blot: PARP2 Antibody [NB100-185] - Detection of PARP2 in NIH/3T3 cell lysate with polyclonal PARP2 antibody at a dilution of 1:250](http://images.novusbio.com/fullsize/PARP2-Antibody-Western-Blot-NB100-185-img0001.jpg "Western Blot: PARP2 Antibody [NB100-185] - Detection of PARP2 in NIH/3T3 cell lysate with polyclonal PARP2 antibody at a dilution of 1:250")
![Western Blot: PARP2 Antibody [NB100-185] - Western blot of analysis of PARP2 in 3T3 cell lysate using NB100-185 at 1:250 dilution.](http://images.novusbio.com/fullsize/PARP2-Antibody-Western-Blot-NB100-185-img0002.jpg)
![Western Blot: PARP2 Antibody [NB100-185] - Activation of signaling pathways by dandelion root extract. Western blots of proteins involved in programmed cell death and cell survival and inflammation. The protein levels, including ULK1 (NBP2-24738), Bcl-2 related protein A1 (NBP1-76715), and PARP2 (NB100-185) corresponded to the mRNA levels obtained during the gene expression analysis. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27564258/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nb100-185_rabbit-polyclonal-parp2-antibody-unpurified-25520231539510.jpg)
![Western Blot: Glutamine Synthetase Antibody (1F4) [NBP2-02125] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Glutamine Synthetase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Western-Blot-NBP2-02125-img0009.jpg "Western Blot: Glutamine Synthetase Antibody (1F4) [NBP2-02125] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Glutamine Synthetase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: Glutamine Synthetase Antibody (OTI1F4) [NBP2-02125] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Glutamine Synthetase.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Immunocytochemistry-Immunofluorescence-NBP2-02125-img0012.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - Staining of paraffin-embedded Human Ovary tissue using anti-Glutamine Synthetase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Immunohistochemistry-Paraffin-NBP2-02125-img0015.jpg)
![Flow Cytometry: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - Analysis of Jurkat cells, using anti-Glutamine Synthetase antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Flow-Cytometry-NBP2-02125-img0011.jpg)
![Western Blot: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Glutamine Synthetase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Glutamine Synthetase.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Western-Blot-NBP2-02125-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - Immunofluorescent staining of HepG2 cells using anti-Glutamine Synthetase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Immunocytochemistry-Immunofluorescence-NBP2-02125-img0004.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - Staining of paraffin-embedded Human breast tissue using anti-Glutamine Synthetase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Immunohistochemistry-Paraffin-NBP2-02125-img0013.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - Staining of paraffin-embedded Human endometrium tissue using anti-Glutamine Synthetase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Immunohistochemistry-Paraffin-NBP2-02125-img0014.jpg)
![Flow Cytometry: Glutamine Synthetase Antibody (1F4) [NBP2-02125] - Analysis of Hela cells, using anti-Glutamine Synthetase antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-1F4-Flow-Cytometry-NBP2-02125-img0010.jpg)
![Western Blot: AKT3 Antibody (OTI9B2) [NBP1-47657] - Analysis of extracts (10ug) from 2 different cell lines by using anti-AKT3 monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/AKT3-Antibody-OTI9B2-Western-Blot-NBP1-47657-img0006.jpg "Western Blot: AKT3 Antibody (OTI9B2) [NBP1-47657] - Analysis of extracts (10ug) from 2 different cell lines by using anti-AKT3 monoclonal antibody at 1:200 dilution.")
![Immunocytochemistry/Immunofluorescence: AKT3 Antibody (9B2) [NBP1-47657] Staining of COS7 cells transiently transfected by pCMV6-ENTRY AKT3.](http://images.novusbio.com/fullsize/AKT3-Antibody-9B2-Immunocytochemistry-Immunofluorescence-NBP1-47657-img0005.jpg)
![Immunohistochemistry-Paraffin: AKT3 Antibody (9B2) [NBP1-47657] - Staining of paraffin-embedded Human lung tissue using anti-AKT3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AKT3-Antibody-9B2-Immunohistochemistry-Paraffin-NBP1-47657-img0004.jpg)
![Flow Cytometry: AKT3 Antibody (9B2) [NBP1-47657] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AKT3 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/AKT3-Antibody-9B2-Flow-Cytometry-NBP1-47657-img0002.jpg)
![Western Blot: AKT3 Antibody (9B2) [NBP1-47657] - AKT3 antibody ( 9B2 ) at 1:500 dilution + BALB/C 3T3 cell lysate](http://images.novusbio.com/fullsize/AKT3-Antibody-9B2-Western-Blot-NBP1-47657-img0001.jpg)
![Western Blot: AKT3 Antibody (9B2) [NBP1-47657] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT3.](http://images.novusbio.com/fullsize/AKT3-Antibody-9B2-Western-Blot-NBP1-47657-img0003.jpg)
![Western Blot: AHR Antibody (RPT1) [NB300-530] - Analysis of 20ug of HepG2, U2OS, and K562 whole cell lysates and 5uL of PageRuler Plus Prestained Protein Ladder per well.](http://images.novusbio.com/fullsize/AHR-Antibody-RPT1-Western-Blot-NB300-530-img0013.jpg "Western Blot: AHR Antibody (RPT1) [NB300-530] - Analysis of 20ug of HepG2, U2OS, and K562 whole cell lysates and 5uL of PageRuler Plus Prestained Protein Ladder per well.")
![Immunocytochemistry/Immunofluorescence: AHR Antibody (RPT1) [NB300-530] - Analysis of Aryl Hydrocarbon Receptor in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Aryl Hydrocarbon Receptor monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Aryl Hydrocarbon Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/AHR-Antibody-RPT1-Immunocytochemistry-Immunofluorescence-NB300-530-img0010.jpg)
![Immunohistochemistry-Paraffin: AHR Antibody (RPT1) [NB300-530] - Cancer biopsies of deparaffinized Human breast carcinoma tissue at a dilution of 1:100.](http://images.novusbio.com/fullsize/AHR-Antibody-RPT1-Immunohistochemistry-Paraffin-NB300-530-img0012.jpg)
![Western Blot: AHR Antibody (RPT1) [NB300-530] - Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an Aryl Hydrocarbon Receptor monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:20,000 for 1 hour.](http://images.novusbio.com/fullsize/AHR-Antibody-RPT1-Western-Blot-NB300-530-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: AHR Antibody (RPT1) [NB300-530] - Analysis of Aryl Hydrocarbon Receptor in NIH-3T3 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Aryl Hydrocarbon Receptor monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Aryl Hydrocarbon Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/AHR-Antibody-RPT1-Immunocytochemistry-Immunofluorescence-NB300-530-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: AHR Antibody (RPT1) [NB300-530] - Analysis of Aryl Hydrocarbon Receptor in MCF-7 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Aryl Hydrocarbon Receptor monoclonal antibody at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Aryl Hydrocarbon Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/AHR-Antibody-RPT1-Immunocytochemistry-Immunofluorescence-NB300-530-img0009.jpg)
![Immunohistochemistry-Paraffin: AHR Antibody (RPT1) [NB300-530] - Normal biopsies of deparaffinized Human skeletal muscle tissue at a dilution of 1:20.](http://images.novusbio.com/fullsize/AHR-Antibody-RPT1-Immunohistochemistry-Paraffin-NB300-530-img0011.jpg)
![Immunohistochemistry: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation of Beclin 1 in Rat Spleen Tissue. Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-Beclin 1 antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76648_rabbit-polyclonal-beclin-1-antibody-2711202420175183.gif "Immunohistochemistry: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation of Beclin 1 in Rat Spleen Tissue. Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-Beclin 1 antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
![Immunohistochemistry: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation of Beclin 1 in Rat Liver Tissue. Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Beclin 1 antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76648_rabbit-polyclonal-beclin-1-antibody-2711202419261860.gif)
![Immunohistochemistry: Beclin 1 Antibody [NBP1-76648] - Beclin-1 in human brain tissue with Beclin-1 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Immunohistochemistry-NBP1-76648-img0004.jpg)
![Immunohistochemistry: Beclin 1 Antibody [NBP1-76648] - Beclin-1 in mouse brain tissue with Beclin-1 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Immunohistochemistry-NBP1-76648-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation of Beclin 1 in Mouse Brain Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain cells labeling Beclin 1 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).](http://images.novusbio.com/fullsize/nbp1-76648_rabbit-polyclonal-beclin-1-antibody-2711202419451274.gif)
![Immunohistochemistry: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation of Beclin 1 in Mouse Kidney Tissue.Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Beclin 1 antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76648_rabbit-polyclonal-beclin-1-antibody-271120241958491.gif)
![Immunocytochemistry/ Immunofluorescence: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation of Beclin 1 in HELA Cells.Immunofluorescent analysis of 4% paraformaldehyde-fixed Hela cells labeling Beclin 1 at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).](http://images.novusbio.com/fullsize/nbp1-76648_rabbit-polyclonal-beclin-1-antibody-2711202419251598.gif)
![Western Blot: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation in Human Cell Lines.Loading: 15 ug of lysate Antibodies: Beclin 1, 1 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76648_rabbit-polyclonal-beclin-1-antibody-271120242016947.gif)
![Western Blot: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation in Mouse Cell Lines.Loading: 15 ug of lysate Antibodies: Beclin 1, 1 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76648_rabbit-polyclonal-beclin-1-antibody-2711202420115589.gif)
![Western Blot: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation in Rat Cell Lines.Loading: 15 ug of lysate Antibodies: Beclin 1, 1 ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76648_rabbit-polyclonal-beclin-1-antibody-2711202419592041.gif)
![Western Blot: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis using PLIN2 mouse mAb against HepG2 cell lysate.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Western-Blot-NBP2-37300-img0004.jpg "Western Blot: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis using PLIN2 mouse mAb against HepG2 cell lysate.")
![Immunocytochemistry/Immunofluorescence: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Immunofluorescence analysis of HepG2 cells using PLIN2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Immunocytochemistry-Immunofluorescence-NBP2-37300-img0005.jpg)
![Immunohistochemistry-Paraffin: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis of bladder cancer tissues using PLIN2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Immunohistochemistry-NBP2-37300-img0008.jpg)
![Flow Cytometry: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis of HepG2 cells using PLIN2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Flow-Cytometry-NBP2-37300-img0006.jpg)
![Western Blot: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis using PLIN2 mAb against human PLIN2 (AA: 286-437) recombinant protein. (Expected MW is 42.6 kDa)](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Western-Blot-NBP2-37300-img0002.jpg)
![Western Blot: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis using PLIN2 mAb against HEK293 (1) and PLIN2 (AA: 286-437)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Western-Blot-NBP2-37300-img0003.jpg)
![Immunohistochemistry-Paraffin: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis of rectum cancer tissues using PLIN2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Immunohistochemistry-NBP2-37300-img0007.jpg)
![ELISA: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-ELISA-NBP2-37300-img0001.jpg)
![Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of GSK-3 alpha/beta [p Tyr216, p Tyr279] on mouse brain tissue lysates with Rabbit anti-GSK-3 alpha/beta [p Tyr216, p Tyr279] antibody at 1:500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 46/51 kDa Observed band size: 46/51 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1:500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Western-Blot-NBP2-67441-img0013.jpg "Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of GSK-3 alpha/beta [p Tyr216, p Tyr279] on mouse brain tissue lysates with Rabbit anti-GSK-3 alpha/beta [p Tyr216, p Tyr279] antibody at 1:500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 46/51 kDa Observed band size: 46/51 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1:500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Staining Phospho-GSK3(alpha+beta)(Y216+Y279) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunocytochemistry-Immunofluorescence-NBP2-67441-img0003.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded human thyroid carcinoma tissue with Rabbit anti-Phospho-GSK3 alpha/beta [p Tyr216, p Tyr279) antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0017.jpg)
![Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of Phospho-GSK3(alpha+beta)(Y216+Y279) on mouse cerebellum tissue lysates. Lane 1: mouse cerebellum tissue, whole cell lysate, 20ug/laneLane 2: mouse cerebellum tissue treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 20ug/lane All lanes : Anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 47/51 kDaObserved band size: 47/51 kDaBlocking and diluting buffer: 5% BSA.Exposure time: 2 minutes](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Western-Blot-NBP2-67441-img0010.jpg)
![Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of Phospho-GSK3(alpha+beta)(Y216+Y279) on 293T cell lysates.Lane 1: 293T cells, whole cell lysate, 10ug/lane Lane 2: 293T cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes :Anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 47/51 kDaObserved band size: 47/51 kDaBlocking and diluting buffer: 5% BSA.Exposure time: 2 minutes](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Western-Blot-NBP2-67441-img0011.jpg)
![Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of Phospho-GSK3(alpha+beta)(Y216+Y279) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: A431 cell lysate Lane 2: Hela cell lysate](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Western-Blot-NBP2-67441-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Staining Phospho-GSK3(alpha+beta)(Y216+Y279) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunocytochemistry-Immunofluorescence-NBP2-67441-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Staining Phospho-GSK3(alpha+beta)(Y216+Y279) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunocytochemistry-Immunofluorescence-NBP2-67441-img0002.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0004.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded mouse brain tissue using anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0005.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded rat brain tissue using anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0006.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded rat brain tissue with Rabbit anti-Phospho-GSK-3 alpha/beta (p Tyr216+Y279) antibody000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0014.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-GSK-3 alpha/beta [p Tyr216, p Tyr279) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0015.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-GSK3 alpha/beta [p Tyr216, p Tyr279] antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0016.jpg)