| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44978 | |||
| Azide and BSA Free | NBP2-34605-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38774-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47949-0.1mg | |||
| IHC-Prediluted | NBP2-48190 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56150SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20774 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-60735SS | |||
| Azide and BSA Free | NBP2-80831 | |||
![Immunohistochemistry-Paraffin: CREB Antibody (LB9) [NB100-74393] - Normal biopsies of deparaffinized Human tonsil tissue.](http://images.novusbio.com/fullsize/CREB-Antibody-LB9-Immunohistochemistry-Paraffin-NB100-74393-img0019.jpg)
![<b>Biological Strategies Validation. </b>Chromatin Immunoprecipitation: CREB Antibody (LB9) [NB100-74393] - Staining of CREB (green) in untreated HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. Cells were then blocked with 5% normal goat serum for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing CREB at a dilution of 1:400 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/CREB-Antibody-LB9-Chromatin-Immunoprecipitation-NB100-74393-img0025.jpg)
![Western Blot: CREB Antibody (LB9) [NB100-74393] - Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing CREB at a dilution of 1:500 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed.](http://images.novusbio.com/fullsize/CREB-Antibody-LB9-Western-Blot-NB100-74393-img0021.jpg)
![Western Blot: CREB Antibody (LB9) [NB100-74393] - Analysis of HepG2 cells](http://images.novusbio.com/fullsize/CREB-Antibody-LB9-Western-Blot-NB100-74393-img0022.jpg)
![Immunohistochemistry-Paraffin: CREB Antibody (LB9) [NB100-74393] - Cancer biopsies of deparaffinized human bladder and rectal tissue.](http://images.novusbio.com/fullsize/CREB-Antibody-LB9-Immunohistochemistry-Paraffin-NB100-74393-img0018.jpg)
![<b>Biological Strategies Validation. </b>Chromatin Immunoprecipitation: CREB Antibody (LB9) [NB100-74393] - Analysis of CREB (green) in untreated HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. Cells were then blocked with 5% normal goat serum for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing CREB at a dilution of 1:500 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei were stained with Hoechst 33342 dye. Images were taken on a Thermo Scientific ArrayScan at 20X magnification.](http://images.novusbio.com/fullsize/CREB-Antibody-LB9-Chromatin-Immunoprecipitation-NB100-74393-img0024.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: CREB Antibody (LB9) [NB100-74393] - Analysis of CREB was performed on untreated 293T cells. Antigen: antbody complex was formed by binding 500ug whole cell lysate with 2ug of mouse monoclonal antibody recognizing CREB overnight on a rocking platform at 4C. The immune-complex was then captured on 50ul Protein A/G Plus Agarose. Captured immune-complexes were then washed extensively and proteins eluted with 5X Reducing Sample Loading Dye. Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to PVDF membrane and blocked with 5% Milk/TBS-0.1%Tween for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing CREB at a dilution of 1:1000 overnight rotating at 4C. Membranes were then washed in TBST and probed with a goat anti-mouse-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Super Signal West Dura.](http://images.novusbio.com/fullsize/CREB-Antibody-LB9-Immunoprecipitation-NB100-74393-img0023.jpg)
![Western Blot: EBI3 Antibody (9J1H4) [NBP3-15735] - Western blot analysis of extracts of MCF7 cells, using EBI3 Rabbit mAb (NBP3-15735) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/EBI3-Antibody-9J1H4-Western-Blot-NBP3-15735-img0004.jpg "Western Blot: EBI3 Antibody (9J1H4) [NBP3-15735] - Western blot analysis of extracts of MCF7 cells, using EBI3 Rabbit mAb (NBP3-15735) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: EBI3 Antibody (9J1H4) [NBP3-15735] - Western blot analysis of extracts of various cell lines, using EBI3 Rabbit mAb (NBP3-15735) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/EBI3-Antibody-9J1H4-Western-Blot-NBP3-15735-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: EBI3 Antibody (9J1H4) [EBI3] - Immunofluorescence analysis of paraffin-embedded rat spleen using EBI3 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15735_rabbit-ebi3-mab-9j1h4-5220251733557.jpg)
![Immunocytochemistry/ Immunofluorescence: EBI3 Antibody (9J1H4) [EBI3] - Immunofluorescence analysis of paraffin-embedded human spleen using EBI3 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15735_rabbit-ebi3-mab-9j1h4-52202517361216.jpg)
![Immunocytochemistry/ Immunofluorescence: EBI3 Antibody (9J1H4) [EBI3] - Immunofluorescence analysis of paraffin-embedded mouse spleen using EBI3 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15735_rabbit-ebi3-mab-9j1h4-522025173357.jpg)
![Simple Western: Beclin 1 Antibody Pack [NB910-95609] - Lane view shows a specific band for Beclin in 1.0 mg/ml of HeLa lysate using NB110-87318. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-Simple-Western-NB910-95609-img0005.jpg "Simple Western: Beclin 1 Antibody Pack [NB910-95609] - Lane view shows a specific band for Beclin in 1.0 mg/ml of HeLa lysate using NB110-87318. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Beclin 1 Antibody Pack [NB910-95609] - Expression of Beclin 1 mRNA and protein in cells and tissues. (A) Beclin 1 mRNA expression level up-regulated in gastric cancer cell lines compared with normal gastric epithelial cells by reverse transcription-PCR. (B) Beclin 1 protein expression level up-regulated in gastric cancer cell lines compared with normal gastric epithelial cells by western blotting. (C) Beclin 1 mRNA expression is elevated in primary gastric tumors compared with paired gastric adjacent noncancerous tissues by reverse transcription-PCR. (D) Beclin 1 protein expression is elevated in primary gastric tumors compared with paired gastric adjacent noncancerous tissues by western blotting. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0045968) licensed under a CC-BY license. Anti-Beclin-1 Antibody NB500-249](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-Western-Blot-NB910-95609-img0013.jpg)
![Immunohistochemistry: Beclin 1 Antibody Pack [NB910-95609] - Analysis of a FFPE tissue section of human breast cancer using Beclin 1 antibody (NB110-87318) at 1:300 dilution. The primary antibody bound to Beclin 1 antigen in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. Cytoplasmic staining can be seen in the cancer as well as the stromal cells. The signal was strongest in a subset of stromal cells which appears to be the cancer associated fibroblasts.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-Immunohistochemistry-NB910-95609-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Beclin 1 Antibody Pack [NB910-95609] - Beclin 1 antibody (NB500-249) was tested in HeLa cells with Dylight 488 (green, left panel). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red), shown in the overlay (right panel)](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB910-95609-img0010.jpg)
![Beclin 1 Antibody Pack [NB910-95609] - Detecting Beclin 1 using NB500-249 in Pheochromocytes of the Adrenal Medulla at 40x.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-NB910-95609-img0011.jpg)
![Western Blot: Beclin 1 Antibody Pack [NB910-95609] - Detection of Beclin 1 in liver lysates using NB500-266. (1) mouse liver and (2) human liver.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-Western-Blot-NB910-95609-img0014.jpg)
![Western Blot: Beclin 1 Antibody Pack [NB910-95609] - Analysis of Beclin1 using NB500-249. Lane 1: human brain. Lane 2: mouse brain.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Pack-Western-Blot-NB910-95609-img0006.jpg)
![Western Blot: p53 Antibody [NBP2-19667] - p53 antibody [N1], N-term detects p53 protein at nucleus by immunofluorescent analysis.<br></br>Samples: HCT 116 cells mock (left) and treated with 30 uM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.<br>Green: p53 protein stained by p53 antibody [N1], N-term.<br>Blue: Hoechst 33342 staining.<br>Scale bar = 10 um.](http://images.novusbio.com/fullsize/p53-Antibody-Western-Blot-NBP2-19667-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: p53 Antibody [NBP2-19667] - p53 antibody [N1], N-term detects p53 protein at nucleus by immunofluorescent analysis.<br></br>Samples: HCT 116 cells mock (left) and treated with 30 uM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.<br>Green: p53 protein stained by p53 antibody [N1], N-term.<br>Blue: Hoechst 33342 staining.<br>Scale bar = 10 um.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: p53 Antibody [NBP2-19667] - Confocal immunofluorescence analysis of paraformaldehyde-fixed U2OS, using p53 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/p53-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19667-img0003.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: p53 Antibody [NBP2-19667] - Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2. 5 ug of anti-p53 antibody. (A) Input, 20ug of HCT116 lysates. The precipitated protein was detected by NBP2-19667. anti-Rabbit IgG Kit was used in the Western blot.](http://images.novusbio.com/fullsize/p53-Antibody-Immunoprecipitation-NBP2-19667-img0007.jpg)
![Whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [N1], N-term (NBP2-19667) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19667_rabbit-polyclonal-p53-antibody-western-blot-2611202518354313.jpg)
![Western Blot: p53 Antibody (DO-7) [NBP2-34308] - Western Blot Analysis of human HeLa cell lysate using p53 Mouse Monoclonal Antibody (DO-7).](http://images.novusbio.com/fullsize/p53-Antibody-DO-7-Western-Blot-NBP2-34308-img0004.jpg "Western Blot: p53 Antibody (DO-7) [NBP2-34308] - Western Blot Analysis of human HeLa cell lysate using p53 Mouse Monoclonal Antibody (DO-7).")
![Immunohistochemistry-Paraffin: p53 Antibody (DO-7) [NBP2-34308] - Formalin-fixed, paraffin-embedded human colon stained with p53 MAb (DO-7)](http://images.novusbio.com/fullsize/p53-Antibody-DO-7-Immunohistochemistry-Paraffin-NBP2-34308-img0001.jpg)
![Simple Western: p53 Antibody (DO-7) [NBP2-34308] - Simple Western lane view shows a specific band for p53 in 0.2 mg/ml of MDA-MB-231 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p53-Antibody-DO-7-Simple-Western-NBP2-34308-img0002.jpg)
![Simple Western: p53 Antibody (DO-7) [NBP2-34308] - Electropherogram image of the corresponding Simple western lane view. p53 antibody was used at 10 ug/ml dilution on MDA-MB-231 lysate(s) respectively.](http://images.novusbio.com/fullsize/p53-Antibody-DO-7-Simple-Western-NBP2-34308-img0003.jpg)
![Immunohistochemistry-Paraffin: CBP/KAT3A Antibody [NBP2-38774] - Analysis in human endometrium and liver tissues. Corresponding CREBBP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38774-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CBP/KAT3A Antibody [NBP2-38774] - Analysis in human endometrium and liver tissues. Corresponding CREBBP RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CBP/KAT3A Antibody [NBP2-38774] - Staining of human cell line MCF7 shows positivity in nucleus but excluded from the nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38774-img0001.jpg)
![Immunohistochemistry-Paraffin: CBP/KAT3A Antibody [NBP2-38774] - Staining of human liver shows very weak nuclear positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38774-img0002.jpg)
![Immunohistochemistry-Paraffin: CBP/KAT3A Antibody [NBP2-38774] - Staining of human endometrium shows strong nuclear positivity in glandular cells and stromal cells.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38774-img0003.jpg)
![Immunohistochemistry-Paraffin: CBP/KAT3A Antibody [NBP2-38774] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38774-img0004.jpg)
![Immunohistochemistry-Paraffin: CBP/KAT3A Antibody [NBP2-38774] - Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38774-img0005.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (LN-6) [NBP2-44832] - Human Melanoma stained with Vimentin Monoclonal Antibody (LN-6).](http://images.novusbio.com/fullsize/Vimentin-Antibody-LN-6-Immunohistochemistry-Paraffin-NBP2-44832-img0002.jpg "Immunohistochemistry-Paraffin: Vimentin Antibody (LN-6) [NBP2-44832] - Human Melanoma stained with Vimentin Monoclonal Antibody (LN-6).")
![Western Blot: BNIP3 Antibody [NB100-56150] - BNIP3 expression in porcine whole skeletal muscle lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/BNIP3-Antibody-Western-Blot-NB100-56150-img0001.jpg "Western Blot: BNIP3 Antibody [NB100-56150] - BNIP3 expression in porcine whole skeletal muscle lysate. Image from verified customer review.")
![Immunohistochemistry-Paraffin: BNIP3 Antibody - Unpurified [NB100-56150] - BNIP3 Antibody [NB100-56150] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human liver using BNIP3 antibody at 1:1000 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Mitochondrial staining was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/BNIP3-Antibody-Immunohistochemistry-Paraffin-NB100-56150-img0002.jpg)
![Western Blot: AKT1 Antibody (3A3) [NBP1-51602] - Analysis using AKT1 antibody against NIH/3T3 (1), Hela (2),COS7 (3) and Jurkat (4) cell lysate.](http://images.novusbio.com/fullsize/Akt1-Antibody-3A3-Western-Blot-NBP1-51602-img0006.jpg "Western Blot: AKT1 Antibody (3A3) [NBP1-51602] - Analysis using AKT1 antibody against NIH/3T3 (1), Hela (2),COS7 (3) and Jurkat (4) cell lysate.")
![Immunohistochemistry-Paraffin: AKT1 Antibody (3A3) [NBP1-51602] - Analysis of human breast carcinoma using AKT1 antibody at 1:50. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Akt1-Antibody-3A3-Immunohistochemistry-Paraffin-NBP1-51602-img0010.jpg)
![Western Blot: AKT1 Antibody (3A3) [NBP1-51602] - Analysis of extracts from COS cells using AKT1 antibody at 1:1000. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Akt1-Antibody-3A3-Western-Blot-NBP1-51602-img0008.jpg)
![Immunohistochemistry-Paraffin: AKT1 Antibody (3A3) [NBP1-51602] - Analysis of human endometrial cancer using AKT1 antibody at 1:25. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Akt1-Antibody-3A3-Immunohistochemistry-Paraffin-NBP1-51602-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (4H11[APG]) [NB500-608] - Human chronic myeloid leukemia cell line MOLM-7 with anti-human CD34 (4H11[APG]) PE.](http://images.novusbio.com/fullsize/CD34-Antibody-4H11-APG-Immunocytochemistry-Immunofluorescence-NB500-608-img0003.jpg "Immunocytochemistry/Immunofluorescence: CD34 Antibody (4H11[APG]) [NB500-608] - Human chronic myeloid leukemia cell line MOLM-7 with anti-human CD34 (4H11[APG]) PE.")
![Flow (Cell Surface): CD34 Antibody (4H11[APG]) [NB500-608] - Surface staining of CD34+ cells in human peripheral blood](http://images.novusbio.com/fullsize/CD34-Antibody-4H11-APG-Flow-Cell-Surface-NB500-608-img0010.jpg)
![Flow (Cell Surface): CD34 Antibody (4H11[APG]) [NB500-608] - Analysis using the FITC conjugate of NB500-608. Staining of Kg-1a human acute myelogenous leukemia cell line with anti-human CD34 (4H11[APG]) FITC. Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/CD34-Antibody-4H11-APG-Flow-Cell-Surface-NB500-608-img0002.jpg)
![Flow Cytometry: CD34 Antibody (4H11[APG]) [NB500-608] - Staining of CD34+ cells in human peripheral blood with anti-human CD34 (4H11[APG]) Alexa Fluor® 700.](http://images.novusbio.com/fullsize/CD34-Antibody-4H11-APG-Flow-Cytometry-NB500-608-img0004.jpg)
![Flow (Cell Surface): CD34 Antibody (4H11[APG]) [NB500-608] - Surface staining of CD34+ cells in human peripheral blood with anti-human CD34 (4H11[APG]) Pacific BlueTM](http://images.novusbio.com/fullsize/CD34-Antibody-4H11-APG-Flow-Cell-Surface-NB500-608-img0005.jpg)
![Flow (Cell Surface): CD34 Antibody (4H11[APG]) [NB500-608] - Surface staining of CD34+ cells in human peripheral blood with anti-human CD34 (4H11[APG]) PE-CyTM7.](http://images.novusbio.com/fullsize/CD34-Antibody-4H11-APG-Flow-Cell-Surface-NB500-608-img0006.jpg)
![Flow (Cell Surface): CD34 Antibody (4H11[APG]) [NB500-608] - Surface staining of CD34+ cells in human peripheral blood with anti-human CD34 (4H11[APG]) Alexa Fluor® 488.](http://images.novusbio.com/fullsize/CD34-Antibody-4H11-APG-Flow-Cell-Surface-NB500-608-img0007.jpg)
![Flow (Cell Surface): CD34 Antibody (4H11[APG]) [NB500-608] - Surface staining of CD34+ cells in human peripheral blood with anti-human CD34 (4H11[APG]) APC.](http://images.novusbio.com/fullsize/CD34-Antibody-4H11-APG-Flow-Cell-Surface-NB500-608-img0008.jpg)
![Flow (Cell Surface): CD34 Antibody (4H11[APG]) [NB500-608] - Surface staining of CD34+ cells in human peripheral blood with anti-CD34 (4H11[APG]) biotin / streptavidin-APC.](http://images.novusbio.com/fullsize/CD34-Antibody-4H11-APG-Flow-Cell-Surface-NB500-608-img0009.jpg)
![Western Blot: LC3B Antibody (1251B) [NBP2-60735] - Western blot analysis of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50uM Chloroquine for 18 hours. PVDF (polyvinylidene difluoride) membrane was probed with 0.1 ug/mL of anti-LC3B monoclonal antibody (Catalog # NBP2-60735) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Knockout-Validated-NBP2-60735-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: LC3B Antibody (1251B) [NBP2-60735] - Western blot analysis of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50uM Chloroquine for 18 hours. PVDF (polyvinylidene difluoride) membrane was probed with 0.1 ug/mL of anti-LC3B monoclonal antibody (Catalog # NBP2-60735) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Simple Western: LC3B Antibody (1251B) [NBP2-60735] - Lane view shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 50uM Chloroquine for 18 hours, loaded at 0.2 mg/mL. A specific band was detected for LC3B at approximately 17 kDa (as indicated) using 5 ug/mL of Rabbit Anti-Human/Mouse/Rat anti-LC3B Monoclonal Antibody (1251B) [Catalog # NBP2-60735]. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Simple-Western-NBP2-60735-img0006.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: LC3B Antibody (1251B) [NBP2-60735] - HeLa cells were treated with 50uM CQ (Chloroquine) overnight, fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti- [Catalog # NBP2-60735] at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Immunocytochemistry-Immunofluorescence-NBP2-60735-img0007.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: LC3B Antibody (1251B) [NBP2-60735] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine (CQ) for 24 hours was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF (Polyvinylidene difluoride) membrane and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 12.0 ug/ml anti-LC3B Antibody (1251B) [Catalog # NBP2-60735] in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Western-Blot-NBP2-60735-img0010.jpg)
![Immunohistochemistry-Paraffin: LC3B Antibody (1251B) [NBP2-60735] - Analysis of a FFPE (Formalin-Fixed Paraffin-Embedded) tissue section of mouse brain using anti-LC3B antibody (clone 1251B) [Catalog # NBP2-60735] at 5ug/ml concentration (1:200 dilution). The primary antibody binding to LC3 in cells was detected using HRP conjugated anti-Rabbit secondary antibody with DAB (3,3'Diaminobenzidine) reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This LC3 antibody generated a diffused staining in all cell types (except the endothelial cells of blood vessels) and the signal was strongest in a subset of neuronal cells. A few cells depicted LC3 puncta formation/dotted staining in apparently autophagic cells.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Immunohistochemistry-Paraffin-NBP2-60735-img0011.jpg)
![<b>Biological Strategies Validation. </b>Flow (Intracellular): LC3B Antibody (1251B) [NBP2-60735] - HeLa cells were either (A) untreated or (B) treated with 50uM chloroquine for 24 hours. An intracellular stain was performed with anti-LC3B (1251B) antibody [Catalog # NBP2-60735] (blue) and a matched isotype control [Catalog # MAB1050] (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Flow-Intracellular-NBP2-60735-img0009.jpg)