| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-81894-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62557-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21183-100ul | |||
| BSA Free | NBP3-21183-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-97515-0.025mg | |||
![Western Blot: Notch-2 Antibody [NBP1-19125] - Whole cell lysate from HeLa and 293T cells. Notch2 was also immunoprecipitated by rabbit anti-Notch2 antibody NBP1-19124.](http://images.novusbio.com/fullsize/Notch-2-Antibody-Western-Blot-NBP1-19125-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Notch-2 Antibody [NBP1-19125] - Whole cell lysate from HeLa and 293T cells. Notch2 was also immunoprecipitated by rabbit anti-Notch2 antibody NBP1-19124.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: Notch-2 Antibody [NBP1-19125] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Notch2 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Notch-2-Antibody-Immunohistochemistry-Paraffin-NBP1-19125-img0003.jpg)
![Immunohistochemistry: SPRY2 Antibody (10M8H1) [NBP3-33538] - Immunohistochemistry analysis of paraffin-embedded Human appendix using SPRY2 Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33538_rabbit-spry2-mab-10m8h1-912202414213721.jpg "Immunohistochemistry: SPRY2 Antibody (10M8H1) [NBP3-33538] - Immunohistochemistry analysis of paraffin-embedded Human appendix using SPRY2 Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.")
![Western Blot: SPRY2 Antibody (10M8H1) [NBP3-33538] - Western blot analysis of lysates from C6 cells, using SPRY2 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-33538_rabbit-spry2-mab-10m8h1-912202415121338.jpg)
![Immunohistochemistry: SPRY2 Antibody (10M8H1) [NBP3-33538] - Immunohistochemistry analysis of paraffin-embedded Rat pancreas using SPRY2 Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33538_rabbit-spry2-mab-10m8h1-912202414201360.jpg)
![Immunohistochemistry: Aggrecan Antibody (6L4T2) [NBP3-33191] - Immunohistochemistry analysis of paraffin-embedded Mouse bone marrow using Aggrecan Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33191_rabbit-aggrecan-mab-6l4t2-912202415133438.jpg "Immunohistochemistry: Aggrecan Antibody (6L4T2) [NBP3-33191] - Immunohistochemistry analysis of paraffin-embedded Mouse bone marrow using Aggrecan Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Western Blot: Aggrecan Antibody (6L4T2) [NBP3-33191] - Western blot analysis of lysates from Mouse lung, using Aggrecan Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-33191_rabbit-aggrecan-mab-6l4t2-91220241514361.jpg)
![Immunohistochemistry: Aggrecan Antibody (6L4T2) [NBP3-33191] - Immunohistochemistry analysis of paraffin-embedded Rat bone marrow using Aggrecan Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33191_rabbit-aggrecan-mab-6l4t2-912202415143624.jpg)
![Western Blot: EGFR [p Tyr1092] Antibody [NB100-81894] - Analysis of extracts from A431 cells untreated or treated with EGF using EGFR(Phospho-Tyr1092) antibody.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr1092]-Antibody-Western-Blot-NB100-81894-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR [p Tyr1092] Antibody [NB100-81894] - Analysis of extracts from A431 cells untreated or treated with EGF using EGFR(Phospho-Tyr1092) antibody.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: EGFR [p Tyr1092] Antibody [NB100-81894] - Analysis of paraffin- embedded human breast carcinoma tissue using NB100-81894(left) or the same antibody preincubated with blocking peptide(right).](http://images.novusbio.com/fullsize/nb100-81894_rabbit-polyclonal-egfr-p-tyr1092-antibody-103202523464545.jpg)
![Flow Cytometry: Notch-1 Antibody (E6) - Chimeric [NBP2-62557] - Flow-cytometry using the anti-Notch 1 antibody E6. Mouse thymocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of E6 (NBP2-62557, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Notch-1-Antibody-E6-Chimeric-Flow-Cytometry-NBP2-62557-img0002.jpg "Flow Cytometry: Notch-1 Antibody (E6) - Chimeric [NBP2-62557] - Flow-cytometry using the anti-Notch 1 antibody E6. Mouse thymocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of E6 (NBP2-62557, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.")
![Immunocytochemistry /Immunofluorescence: Notch-1 Antibody (E6) - Chimeric - Azide and BSA Free [NBP2-62557] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with NBP2-62557 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62557, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-62557_rabbit-monoclonal-notch-1-antibody-e6-chimeric-74202522141235.png)
![Immunohistochemistry-Paraffin: Frizzled-9 Antibody [NB100-94906] - Staining of paraffin embedded Human Thyroid. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Frizzled-9-Antibody-Immunohistochemistry-Paraffin-NB100-94906-img0003.jpg "Immunohistochemistry-Paraffin: Frizzled-9 Antibody [NB100-94906] - Staining of paraffin embedded Human Thyroid. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Immunohistochemistry-Paraffin: Frizzled-9 Antibody [NB100-94906] - Staining of paraffin embedded Human Testis. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Frizzled-9-Antibody-Immunohistochemistry-Paraffin-NB100-94906-img0002.jpg)
![Western Blot: FOXG1 Antibody [NB100-1099] - (1ug/ml) staining of U251 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/nb100-1099_goat-polyclonal-foxg1-antibody-16520239183341.jpg "Western Blot: FOXG1 Antibody [NB100-1099] - (1ug/ml) staining of U251 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Western Blot: FOXG1 Antibody [NB100-1099] - (0.5ug/ml) staining of Human Cerebellum (A) and Cerebral Cortex (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/nb100-1099_goat-polyclonal-foxg1-antibody-16520239183351.jpg)
![Flow Cytometry: FOXG1 Antibody [NB100-1099] - Flow cytometric analysis of paraformaldehyde fixed U251 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nb100-1099_goat-polyclonal-foxg1-antibody-1652023922511.jpg)
![Immunohistochemistry-Paraffin: sFRP-4 Antibody (5#) [NBP3-41642] - DAB staining on IHC-P; Sample: Human Colon Tissue; Primary Ab: 10ug/ml NBP3-41642; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41642_mouse-sfrp-4-mab-5-6122024212721.jpg "Immunohistochemistry-Paraffin: sFRP-4 Antibody (5#) [NBP3-41642] - DAB staining on IHC-P; Sample: Human Colon Tissue; Primary Ab: 10ug/ml NBP3-41642; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Western Blot: sFRP-4 Antibody (5#) [NBP3-41642] - Sample: Lane1: 293T cell lysate; Lane2: Hela cell lysate; Lane3: Porcine Heart lysate; Lane4: SKOV3 cell lysate Primary Ab: 2ug/ml NBP3-41642; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41642_mouse-sfrp-4-mab-5-61220242195744.jpg)
![Western Blot: sFRP-4 Antibody (5#) [NBP3-41642] - Sample: Recombinant sFRP-4, Human.](http://images.novusbio.com/fullsize/nbp3-41642_mouse-sfrp-4-mab-5-6122024212769.jpg)
![Immunocytochemistry/ Immunofluorescence: AKT1/2/3 Antibody (JE75-09) [NBP3-32017] - Immunocytochemistry analysis of MCF7 cells labeling AKT1/2/3 with Rabbit anti-AKT1/2/3 antibody (NBP3-32017) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AKT1/2/3 antibody (NBP3-32017) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32017_rabbit-akt1-2-3-mab-je75-09-29202410334024.jpg "Immunocytochemistry/ Immunofluorescence: AKT1/2/3 Antibody (JE75-09) [NBP3-32017] - Immunocytochemistry analysis of MCF7 cells labeling AKT1/2/3 with Rabbit anti-AKT1/2/3 antibody (NBP3-32017) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AKT1/2/3 antibody (NBP3-32017) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.")
![Flow Cytometry: AKT1/2/3 Antibody (JE75-09) [NBP3-32017] - Flow cytometric analysis of MCF7 cells labeling AKT1/2/3. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32017, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32017_rabbit-akt1-2-3-mab-je75-09-29202416453243.jpg)
![Western Blot: AKT1/2/3 Antibody (JE75-09) [NBP3-32017] - Western blot analysis of AKT1/2/3 on different lysates with Rabbit anti-AKT1/2/3 antibody (NBP3-32017) at 1/2,000 dilution.Lane 1: HeLa cell lysateLane 2: MCF7 cell lysateLane 3: A549 cell lysateLane 4: U-2 OS cell lysateLane 5: NIH/3T3 cell lysateLysates/proteins at 15 µg/Lane.Predicted band size: 56 kDaObserved band size: 56 kDaExposure time: 30 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32017_rabbit-akt1-2-3-mab-je75-09-3920248453538.jpg)
![Western Blot: Amphiregulin Antibody [NBP2-15379] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Amphiregulin antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Amphiregulin-Antibody-Western-Blot-NBP2-15379-img0003.jpg "Western Blot: Amphiregulin Antibody [NBP2-15379] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Amphiregulin antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Amphiregulin Antibody [NBP2-15379] - Paraffin-embedded human colon cancer. Amphiregulin antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Amphiregulin-Antibody-Immunohistochemistry-Paraffin-NBP2-15379-img0001.jpg)
![Western Blot: Vimentin [p Ser71] Antibody (TM71) [NBP1-97515] - Analysis of phosphorylated vimentin (Ser71) expression in U251 using [pSer71]Vimentin, mAb (TM71).](http://images.novusbio.com/fullsize/Vimentin-p-Ser71-Antibody-TM71-Western-Blot-NBP1-97515-img0001.jpg "Western Blot: Vimentin [p Ser71] Antibody (TM71) [NBP1-97515] - Analysis of phosphorylated vimentin (Ser71) expression in U251 using [pSer71]Vimentin, mAb (TM71).")
![Immunocytochemistry/Immunofluorescence: Vimentin [p Ser71] Antibody (TM71) [NBP1-97515] - Analysis of phosphorylated vimentin (Ser71) on 4% PFA fixed U251 cells with [pSer71]Vimentin, mAb (TM71).](http://images.novusbio.com/fullsize/Vimentin-p-Ser71-Antibody-TM71-Immunohistochemistry-NBP1-97515-img0002.jpg)