| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-04084-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61693-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2718SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-97515-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98104-50ul | |||
![Western Blot: Integrin alpha 4/CD49d Antibody (8M8C2) [NBP3-16322] - Analysis of extracts of various cell lines, using Integrin alpha 4/CD49d (ITGA4/CD49d) Rabbit mAb (NBP3-16322) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Integrin-alpha-4-CD49d-Antibody-8M8C2-Western-Blot-NBP3-16322-img0002.jpg "Western Blot: Integrin alpha 4/CD49d Antibody (8M8C2) [NBP3-16322] - Analysis of extracts of various cell lines, using Integrin alpha 4/CD49d (ITGA4/CD49d) Rabbit mAb (NBP3-16322) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: Integrin alpha 4/CD49d Antibody (8M8C2) [NBP3-16322] - Mouse testis using Integrin alpha 4/CD49d (ITGA4/CD49d) Rabbit mAb (NBP3-16322) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Integrin-alpha-4-CD49d-Antibody-8M8C2-Immunohistochemistry-Paraffin-NBP3-16322-img0001.jpg)
![Immunohistochemistry: AKT1/2 Antibody (1G0K5) [NBP3-33526] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using AKT1/2 Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33526_rabbit-akt1-2-mab-1g0k5-91220241519579.jpg "Immunohistochemistry: AKT1/2 Antibody (1G0K5) [NBP3-33526] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using AKT1/2 Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Western Blot: AKT1/2 Antibody (1G0K5) [NBP3-33526] - Western blot analysis of various lysates using AKT1/2 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-33526_rabbit-akt1-2-mab-1g0k5-912202415952.jpg)
![Western Blot: 14-3-3 gamma Antibody (5R4Q4) [NBP3-16771] - Western blot analysis of extracts of various cell lines, using 14-3-3 gamma Rabbit mAb (NBP3-16771) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-5R4Q4-Western-Blot-NBP3-16771-img0002.jpg "Western Blot: 14-3-3 gamma Antibody (5R4Q4) [NBP3-16771] - Western blot analysis of extracts of various cell lines, using 14-3-3 gamma Rabbit mAb (NBP3-16771) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: 14-3-3 gamma Antibody (5R4Q4) [NBP3-16771] - Immunohistochemistry of paraffin-embedded mouse brain using 14-3-3 gamma Rabbit mAb (NBP3-16771) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-5R4Q4-Immunohistochemistry-Paraffin-NBP3-16771-img0001.jpg)
![Western Blot: FOXG1 Antibody [NBP3-04084] - Analysis of extracts of various cell lines, using FOXG1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/FOXG1-Antibody-Western-Blot-NBP3-04084-img0002.jpg "Western Blot: FOXG1 Antibody [NBP3-04084] - Analysis of extracts of various cell lines, using FOXG1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: FOXG1 Antibody [NBP3-04084] - Analysis of U2OS cells using FOxG1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/FOXG1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-04084-img0001.jpg)
![Western Blot: FOXG1 Antibody [NBP2-81942] - Analysis of FOXG1 in rat brain tissue lysate with FOXG1 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/FOXG1-Antibody-Western-Blot-NBP2-81942-img0001.jpg "Western Blot: FOXG1 Antibody [NBP2-81942] - Analysis of FOXG1 in rat brain tissue lysate with FOXG1 antibody at 1 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: FOXG1 Antibody - BSA Free [NBP2-81942] - Immunofluorescence of FOXG1 in mouse brain tissue with FOXG1 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp2-81942_rabbit-polyclonal-foxg1-antibody-271120241928858.jpg)
![Western Blot: CD16/CD32 Antibody (2G10A9) [NBP2-61693] - Analysis using CD16 mAb against human CD16 (AA: extra 17-208) recombinant protein. (Expected MW is 47.8 kDa)](http://images.novusbio.com/fullsize/CD16-CD32-Antibody-2G10A9-Western-Blot-NBP2-61693-img0003.jpg "Western Blot: CD16/CD32 Antibody (2G10A9) [NBP2-61693] - Analysis using CD16 mAb against human CD16 (AA: extra 17-208) recombinant protein. (Expected MW is 47.8 kDa)")
![Flow Cytometry: CD16/CD32 Antibody (2G10A9) [NBP2-61693] - Analysis of Ramos cells using CD16 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/CD16-CD32-Antibody-2G10A9-Flow-Cytometry-NBP2-61693-img0002.jpg)
![Western Blot: CD16/CD32 Antibody (2G10A9) [NBP2-61693] - Analysis using CD16 mAb against HEK293 (1) and CD16 (AA: extra 17-208)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CD16-CD32-Antibody-2G10A9-Western-Blot-NBP2-61693-img0004.jpg)
![ELISA: CD16/CD32 Antibody (2G10A9) [NBP2-61693] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/CD16-CD32-Antibody-2G10A9-ELISA-NBP2-61693-img0001.jpg)
![Western Blot: Vimentin [p Ser71] Antibody (TM71) [NBP1-97515] - Analysis of phosphorylated vimentin (Ser71) expression in U251 using [pSer71]Vimentin, mAb (TM71).](http://images.novusbio.com/fullsize/Vimentin-p-Ser71-Antibody-TM71-Western-Blot-NBP1-97515-img0001.jpg "Western Blot: Vimentin [p Ser71] Antibody (TM71) [NBP1-97515] - Analysis of phosphorylated vimentin (Ser71) expression in U251 using [pSer71]Vimentin, mAb (TM71).")
![Immunocytochemistry/Immunofluorescence: Vimentin [p Ser71] Antibody (TM71) [NBP1-97515] - Analysis of phosphorylated vimentin (Ser71) on 4% PFA fixed U251 cells with [pSer71]Vimentin, mAb (TM71).](http://images.novusbio.com/fullsize/Vimentin-p-Ser71-Antibody-TM71-Immunohistochemistry-NBP1-97515-img0002.jpg)
![Western Blot: Vimentin [p Ser38] Antibody (JE43-26) [NBP3-32816] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-32816) at 1/1,000 dilution. Lane 1: HeLa whole cell lysate Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes whole cell lysate Lane 3: HeLa treated with 100nM Calyculin A for 30 minutes whole cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32816) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32816_rabbit-vimentin-p-ser38-mab-je43-26-288202412442957.jpg "Western Blot: Vimentin [p Ser38] Antibody (JE43-26) [NBP3-32816] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-32816) at 1/1,000 dilution. Lane 1: HeLa whole cell lysate Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes whole cell lysate Lane 3: HeLa treated with 100nM Calyculin A for 30 minutes whole cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32816) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Vimentin [p Ser38] Antibody (JE43-26) [NBP3-32816] - Immunocytochemistry analysis of HeLa cells treated with or without 100nM Calyculin A for 30 minutes, then treated with lambda pp for 1 hour labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-32816) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody (NBP3-32816) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32816_rabbit-vimentin-p-ser38-mab-je43-26-29202410234248.jpg)
![Immunohistochemistry-Paraffin: CELSR2 Antibody [NBP2-98104] - Immunochemical staining of human CELSR2 in human small intestine with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/CELSR2-Antibody-Immunohistochemistry-Paraffin-NBP2-98104-img0003.jpg "Immunohistochemistry-Paraffin: CELSR2 Antibody [NBP2-98104] - Immunochemical staining of human CELSR2 in human small intestine with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: CELSR2 Antibody [NBP2-98104] - Immunochemical staining of human CELSR2 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/CELSR2-Antibody-Immunohistochemistry-Paraffin-NBP2-98104-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CELSR2 Antibody [NBP2-98104] - Immunofluorescence staining of CELSR2 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98104 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98104_rabbit-polyclonal-celsr2-antibody-235202516144342.png)
![Immunohistochemistry: Fc gamma RIII (CD16) Antibody (PD00-12) [NBP3-32127] - Immunofluorescence analysis of paraffin-embedded human spleen tissue labeling Fc gamma RIII (CD16) with Rabbit anti-Fc gamma RIII (CD16) antibody (NBP3-32127) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32127, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32127_rabbit-fc-gamma-riii-cd16-mab-pd00-12-298202414333631.jpg "Immunohistochemistry: Fc gamma RIII (CD16) Antibody (PD00-12) [NBP3-32127] - Immunofluorescence analysis of paraffin-embedded human spleen tissue labeling Fc gamma RIII (CD16) with Rabbit anti-Fc gamma RIII (CD16) antibody (NBP3-32127) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32127, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: Fc gamma RIII (CD16) Antibody (PD00-12) [NBP3-32127] - Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Fc gamma RIII (CD16) antibody (NBP3-32127) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32127) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32127_rabbit-fc-gamma-riii-cd16-mab-pd00-12-29820241435244.jpg)
![Immunocytochemistry/ Immunofluorescence: Fc gamma RIII (CD16) Antibody (PD00-12) [NBP3-32127] - Immunocytochemistry analysis of 293T cells labeling Fc gamma RIII (CD16) with Rabbit anti-Fc gamma RIII (CD16) antibody (NBP3-32127) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Fc gamma RIII (CD16) antibody (NBP3-32127) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32127_rabbit-fc-gamma-riii-cd16-mab-pd00-12-2920241192312.jpg)
![Western Blot: EGF R/ErbB1 [p Tyr992] Antibody (EM-12) [NBP1-44894] - Analysis of EGFR (phospho-Tyr992) by mouse monoclonal antibody EM-12 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).](http://images.novusbio.com/fullsize/EGF-R-ErbB1-p-Tyr992-Antibody-EM-12-Western-Blot-NBP1-44894-img0001.jpg "Western Blot: EGF R/ErbB1 [p Tyr992] Antibody (EM-12) [NBP1-44894] - Analysis of EGFR (phospho-Tyr992) by mouse monoclonal antibody EM-12 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).")
![Immunoprecipitation: EGF R/ErbB1 [p Tyr992] Antibody (EM-12) [NBP1-44894] - EGFR from EGF-treated A431 cells by monoclonal antibodies EM-12 (A), EM-13 (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal (D). The precipitates were immunoblotted with EM-12 or EM-13 antibody, and goat anti-mouse-HRP.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-p-Tyr992-Antibody-EM-12-Immunoprecipitation-NBP1-44894-img0002.jpg)
![Immunoprecipitation: EGF R/ErbB1 [p Tyr992] Antibody (EM-12) [NBP1-44894] - EGFR from EGF-treated A431 cells by phosphospecific monoclonal antibodies EM-12 (A), EM-13 (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal (D). The precipitates were immunoblotted with a commercial anti-EGFR polyclonal and goat anti-rabbit-HRP.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-p-Tyr992-Antibody-EM-12-Immunoprecipitation-NBP1-44894-img0003.jpg)