| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95105-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89702-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-68808-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18571-25ul | |||
| Azide and BSA Free | NBP3-44392 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43924 | |||
| BSA Free | NBP2-52867-25ul | |||
![Western Blot: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Western blot analysis of extracts of various cell lines, using RBBP4/RbAp48 Rabbit mAb (NBP3-16237) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-0D1X1-Western-Blot-NBP3-16237-img0007.jpg "Western Blot: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Western blot analysis of extracts of various cell lines, using RBBP4/RbAp48 Rabbit mAb (NBP3-16237) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunocytochemistry/ Immunofluorescence: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Confocal imaging of NIH/3T3 cells using RBBP4/RbAp48 Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16237_rabbit-rbbp4-rbap48-mab-0d1x1-52202511411412.jpg)
![Immunocytochemistry/ Immunofluorescence: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Confocal imaging of U-2 OS cells using RBBP4/RbAp48 Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16237_rabbit-rbbp4-rbap48-mab-0d1x1-52202512548.jpg)
![Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Immunohistochemistry of paraffin-embedded mouse brain using RBBP4/RbAp48 Rabbit mAb (NBP3-16237) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-0D1X1-Immunohistochemistry-Paraffin-NBP3-16237-img0003.jpg)
![Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Immunohistochemistry of paraffin-embedded rat brain using RBBP4/RbAp48 Rabbit mAb (NBP3-16237) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-0D1X1-Immunohistochemistry-Paraffin-NBP3-16237-img0001.jpg)
![Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Immunohistochemistry of paraffin-embedded human lung cancer using RBBP4/RbAp48 Rabbit mAb (NBP3-16237) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-0D1X1-Immunohistochemistry-Paraffin-NBP3-16237-img0002.jpg)
![Immunohistochemistry: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Immunohistochemistry analysis of paraffin-embedded human breast cancer using RBBP4/RbAp48 Rabbit mAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16237_rabbit-rbbp4-rbap48-mab-0d1x1-522025125555.jpg)
![Immunohistochemistry: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Immunohistochemistry analysis of paraffin-embedded human tonsil using RBBP4/RbAp48 Rabbit mAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16237_rabbit-rbbp4-rbap48-mab-0d1x1-52202512543.jpg)
![Immunohistochemistry: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using RBBP4/RbAp48 Rabbit mAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16237_rabbit-rbbp4-rbap48-mab-0d1x1-5220251215417.jpg)
![Immunohistochemistry: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Immunohistochemistry analysis of paraffin-embedded rat kidney using RBBP4/RbAp48 Rabbit mAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16237_rabbit-rbbp4-rbap48-mab-0d1x1-522025121540.jpg)
![Immunohistochemistry: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using RBBP4/RbAp48 Rabbit mAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16237_rabbit-rbbp4-rbap48-mab-0d1x1-5220251242580.jpg)
![Immunohistochemistry: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Immunohistochemistry analysis of paraffin-embedded human placenta using RBBP4/RbAp48 Rabbit mAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16237_rabbit-rbbp4-rbap48-mab-0d1x1-52202511392291.jpg)
![Immunohistochemistry: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Immunohistochemistry analysis of paraffin-embedded human kidney using RBBP4/RbAp48 Rabbit mAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16237_rabbit-rbbp4-rbap48-mab-0d1x1-52202511383781.jpg)
![Immunohistochemistry: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Immunohistochemistry analysis of paraffin-embedded human colon using RBBP4/RbAp48 Rabbit mAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16237_rabbit-rbbp4-rbap48-mab-0d1x1-5220251141143.jpg)
![Immunohistochemistry: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Immunohistochemistry analysis of paraffin-embedded human liver using RBBP4/RbAp48 Rabbit mAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16237_rabbit-rbbp4-rbap48-mab-0d1x1-5220251242517.jpg)
![Immunohistochemistry: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Immunohistochemistry analysis of paraffin-embedded mouse liver using RBBP4/RbAp48 Rabbit mAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16237_rabbit-rbbp4-rbap48-mab-0d1x1-52202511402783.jpg)
![Immunohistochemistry: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Immunohistochemistry analysis of paraffin-embedded mouse intestin using RBBP4/RbAp48 Rabbit mAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16237_rabbit-rbbp4-rbap48-mab-0d1x1-52202511374173.jpg)
![Western Blot: Notch-1 Antibody [NBP2-95105] - Analysis of extracts of U-251MG cells, using Notch-1 .Exposure time: 300s.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Western-Blot-NBP2-95105-img0006.jpg "Western Blot: Notch-1 Antibody [NBP2-95105] - Analysis of extracts of U-251MG cells, using Notch-1 .Exposure time: 300s.")
![Immunohistochemistry: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using Notch-1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-5220251638038.jpg)
![Immunohistochemistry: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using Notch-1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-522025163964.jpg)
![Immunohistochemistry: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Notch-1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-5220251638034.jpg)
![Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunofluorescence analysis of U2OS cells using Notch-1 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-5220251639698.jpg)
![Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunofluorescence analysis of HeLa cells using Notch-1 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-5220251636390.jpg)
![Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunofluorescence analysis of A-549 cells using Notch-1 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-52202516283218.jpg)
![Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunofluorescence analysis of PC-12 cells using Notch-1 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-5220251638067.jpg)
![Immunohistochemistry: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Notch-1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-522025163633.jpg)
![Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunofluorescence analysis of NIH/3T3 cells using Notch-1 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-52202516345270.jpg)
![Western Blot: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of PEN2 on mouse spleen tissue lysate using anti-PEN2 antibody at 1/2,000 dilution.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Western-Blot-NBP2-75632-img0009.jpg "Western Blot: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of PEN2 on mouse spleen tissue lysate using anti-PEN2 antibody at 1/2,000 dilution.")
![Immunocytochemistry/Immunofluorescence: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Staining PEN2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunocytochemistry-Immunofluorescence-NBP2-75632-img0003.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded rat cerebellum tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0008.jpg)
![Flow Cytometry: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of A549 cells with PEN2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Flow-Cytometry-NBP2-75632-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Staining PEN2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunocytochemistry-Immunofluorescence-NBP2-75632-img0002.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded human lung cancer tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0004.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded human kidney tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0005.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded human tonsil tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0006.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded mouse testis tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0007.jpg)
![Immunoprecipitation: Vimentin Antibody (SC60-05) [NBP3-33094] - Vimentin was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-33094 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-33094 at 1/10,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-33094 in HeLa cell lysate Lane 3: NBP3-33094 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 5 seconds](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-2682024158265.jpg "Immunoprecipitation: Vimentin Antibody (SC60-05) [NBP3-33094] - Vimentin was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-33094 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-33094 at 1/10,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-33094 in HeLa cell lysate Lane 3: NBP3-33094 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 5 seconds")
![Immunohistochemistry-Frozen: Vimentin Antibody (SC60-05) [NBP3-33094] - Immunofluorescence analysis of frozen mouse hippocampus tissue labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33094).The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33094, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-2682024159390.jpg)
![Immunohistochemistry: Vimentin Antibody (SC60-05) [NBP3-33094] - Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma tissue with Rabbit anti-Vimentin antibody (NBP3-33094) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33094) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-298202414161822.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody (SC60-05) [NBP3-33094] - Immunocytochemistry analysis of C6 cells labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33094) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-29202410234261.jpg)
![Flow Cytometry: Vimentin Antibody (SC60-05) [NBP3-33094] - Flow cytometric analysis of HeLa (positive, red) and MCF7 (negative, green) cells labeling Vimentin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33094) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (HeLa black, MCF7 light green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-29202416453228.jpg)
![Western Blot: Vimentin Antibody (SC60-05) [NBP3-33094] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-33094) at 1/20,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: HEK-293 cell lysate (10 µg/Lane) Lane 3: Jurkat cell lysate (10 µg/Lane) Lane 4: C2C12 cell lysate (10 µg/Lane) Lane 5: RAW264.7 cell lysate (10 µg/Lane) Lane 6: C6 cell lysate (10 µg/Lane) Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-3920248453527.jpg)
![ELISA: Human Vimentin ELISA Kit (Chemiluminescence) [NBP2-68168] - Samples were spiked with high concentrations of Human Vimentin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-68168_human-vimentin-elisa-kit-chemiluminescence-132202416102217.jpg "ELISA: Human Vimentin ELISA Kit (Chemiluminescence) [NBP2-68168] - Samples were spiked with high concentrations of Human Vimentin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Vimentin ELISA Kit (Chemiluminescence) [NBP2-68168] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-68168_human-vimentin-elisa-kit-chemiluminescence-4320249133291.jpg)
![Immunocytochemistry/Immunofluorescence: PRRX1 Antibody [NBP2-68808] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PRRX1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-68808-img0001.jpg "Immunocytochemistry/Immunofluorescence: PRRX1 Antibody [NBP2-68808] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunocytochemistry/Immunofluorescence: CDX2 Antibody (CL12967) [NBP3-18571] - Analysis in human cell line CACO-2 and human cell line PC-3.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12967-Immunocytochemistry-Immunofluorescence-NBP3-18571-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CDX2 Antibody (CL12967) [NBP3-18571] - Analysis in human cell line CACO-2 and human cell line PC-3.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12967-Immunohistochemistry-Paraffin-NBP3-18571-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CDX2 Antibody (CL12967) [NBP3-18571] - Staining of CACO-2 cells using the Anti-CDX2 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule probes are visualized in red (where available).](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12967-Immunocytochemistry-Immunofluorescence-NBP3-18571-img0001.jpg)
![Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571] - Analysis in human colon and tonsil tissues using NBP3-18571 antibody. Corresponding CDX2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12967-Immunohistochemistry-Paraffin-NBP3-18571-img0002.jpg)
![Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571] - Staining of human colorectal cancer shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12967-Immunohistochemistry-Paraffin-NBP3-18571-img0003.jpg)
![Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571] - Staining of human colorectal cancer (metastasis to ovary) shows moderate to strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12967-Immunohistochemistry-Paraffin-NBP3-18571-img0004.jpg)
![Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571] - Staining of human colon shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12967-Immunohistochemistry-Paraffin-NBP3-18571-img0005.jpg)
![Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571] - Staining of human rectum shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12967-Immunohistochemistry-Paraffin-NBP3-18571-img0006.jpg)
![Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12967-Immunohistochemistry-Paraffin-NBP3-18571-img0007.jpg)
![Western Blot: Vimentin Antibody (CL0157) [NBP2-52867] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-52867) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Knockout-Validated-NBP2-52867-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody (CL0157) [NBP2-52867] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-52867) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: Vimentin Antibody (CL0157) [NBP2-52867] - Analysis in human cell lines U-251MG and MCF-7 using Anti-VIM antibody. Corresponding VIM RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Western-Blot-NBP2-52867-img0010.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human skin shows distinct positivity in fibroblasts.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Immunohistochemistry-Paraffin-NBP2-52867-img0005.jpg)
![Western Blot: Vimentin Antibody (CL0157) [NBP2-52867] - Lane 1: Marker [kDa] Lane 2: Human tonsil tissue lysate](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Western-Blot-NBP2-52867-img0006.jpg)
![Western Blot: Vimentin Antibody (CL0157) [NBP2-52867] - Lane 1: Marker [kDa], Lane 2: Human cell line HeLa cytoplasmic fraction , Lane 3: Human cell line HeLa membrane fraction , Lane 4: Human cell line HeLa nuclear fraction , Lane 5: Human cell line HeLa chromatin fraction , Lane 6: Human cell line HeLa cytos](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Western-Blot-NBP2-52867-img0007.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human cerebellum shows immunoreactivity in the endothelial cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Immunohistochemistry-Paraffin-NBP2-52867-img0001.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human kidney shows strong immunoreactivity in renal glomeruli but not tubuli cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Immunohistochemistry-Paraffin-NBP2-52867-img0002.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human prostate shows strong immunoreactivity in the connective tissue.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Immunohistochemistry-Paraffin-NBP2-52867-img0003.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human rectum shows strong positivity in mesenchymal and lymphoid cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Immunohistochemistry-Paraffin-NBP2-52867-img0004.jpg)
![Western Blot: DLX3 Antibody [NBP2-24646] - Analysis of DLX3 in NIH 3T3 cell lysates in the 1) absence and 2) presence of immunizing peptide using NBP2-24646 at 3 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/DLX3-Antibody-Western-Blot-NBP2-24646-img0002.jpg "Western Blot: DLX3 Antibody [NBP2-24646] - Analysis of DLX3 in NIH 3T3 cell lysates in the 1) absence and 2) presence of immunizing peptide using NBP2-24646 at 3 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: DLX3 Antibody [NBP2-24646] - Analysis of DLX3 in formalin-fixed, paraffin-embedded mouse testis tissue using this antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/DLX3-Antibody-Immunohistochemistry-Paraffin-NBP2-24646-img0001.jpg)
![Immunohistochemistry-Paraffin: Slit2 Antibody [NBP2-20398] - Human gastric carcinoma. SLIT2 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Slit2-Antibody-Immunohistochemistry-Paraffin-NBP2-20398-img0011.jpg "Immunohistochemistry-Paraffin: Slit2 Antibody [NBP2-20398] - Human gastric carcinoma. SLIT2 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
