| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18552-25ul | |||
| Azide and BSA Free | NBP3-44391 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43924 | |||
| BSA Free | NBP2-52867-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18571-25ul | |||
| Azide and BSA Free | NBP3-44392 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-50056-20ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33068 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84674-25ul | |||
![Immunoprecipitation: Vimentin Antibody (SC60-05) [NBP3-33094] - Vimentin was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-33094 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-33094 at 1/10,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-33094 in HeLa cell lysate Lane 3: NBP3-33094 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 5 seconds](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-2682024158265.jpg "Immunoprecipitation: Vimentin Antibody (SC60-05) [NBP3-33094] - Vimentin was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-33094 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-33094 at 1/10,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-33094 in HeLa cell lysate Lane 3: NBP3-33094 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 5 seconds")
![Immunohistochemistry-Frozen: Vimentin Antibody (SC60-05) [NBP3-33094] - Immunofluorescence analysis of frozen mouse hippocampus tissue labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33094).The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33094, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-2682024159390.jpg)
![Immunohistochemistry: Vimentin Antibody (SC60-05) [NBP3-33094] - Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma tissue with Rabbit anti-Vimentin antibody (NBP3-33094) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33094) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-298202414161822.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody (SC60-05) [NBP3-33094] - Immunocytochemistry analysis of C6 cells labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33094) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-29202410234261.jpg)
![Flow Cytometry: Vimentin Antibody (SC60-05) [NBP3-33094] - Flow cytometric analysis of HeLa (positive, red) and MCF7 (negative, green) cells labeling Vimentin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33094) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (HeLa black, MCF7 light green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-29202416453228.jpg)
![Western Blot: Vimentin Antibody (SC60-05) [NBP3-33094] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-33094) at 1/20,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: HEK-293 cell lysate (10 µg/Lane) Lane 3: Jurkat cell lysate (10 µg/Lane) Lane 4: C2C12 cell lysate (10 µg/Lane) Lane 5: RAW264.7 cell lysate (10 µg/Lane) Lane 6: C6 cell lysate (10 µg/Lane) Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-3920248453527.jpg)
![Western Blot: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of PEN2 on mouse spleen tissue lysate using anti-PEN2 antibody at 1/2,000 dilution.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Western-Blot-NBP2-75632-img0009.jpg "Western Blot: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of PEN2 on mouse spleen tissue lysate using anti-PEN2 antibody at 1/2,000 dilution.")
![Immunocytochemistry/Immunofluorescence: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Staining PEN2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunocytochemistry-Immunofluorescence-NBP2-75632-img0003.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded rat cerebellum tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0008.jpg)
![Flow Cytometry: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of A549 cells with PEN2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Flow-Cytometry-NBP2-75632-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Staining PEN2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunocytochemistry-Immunofluorescence-NBP2-75632-img0002.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded human lung cancer tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0004.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded human kidney tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0005.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded human tonsil tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0006.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded mouse testis tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0007.jpg)
![Western Blot: CDX2 Antibody (CL12974) [NBP3-18552] - Analysis in human cell line CACO-2 and human cell line PC-3.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12974-Western-Blot-NBP3-18552-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: CDX2 Antibody (CL12974) [NBP3-18552] - Analysis in human cell line CACO-2 and human cell line PC-3.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CDX2 Antibody (CL12974) [NBP3-18552] - Staining of CACO-2 cells using the Anti-CDX2 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule is visualized in red (where available).](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12974-Immunocytochemistry-Immunofluorescence-NBP3-18552-img0001.jpg)
![Immunohistochemistry-Paraffin: CDX2 Antibody (CL12974) [NBP3-18552] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12974-Immunohistochemistry-Paraffin-NBP3-18552-img0007.jpg)
![Immunohistochemistry-Paraffin: CDX2 Antibody (CL12974) [NBP3-18552] - Analysis in human rectum and lymph node tissues using NBP3-18552 antibody. Corresponding CDX2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12974-Immunohistochemistry-Paraffin-NBP3-18552-img0002.jpg)
![Immunohistochemistry-Paraffin: CDX2 Antibody (CL12974) [NBP3-18552] - Staining of human colorectal cancer shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12974-Immunohistochemistry-Paraffin-NBP3-18552-img0003.jpg)
![Immunohistochemistry-Paraffin: CDX2 Antibody (CL12974) [NBP3-18552] - Staining of human colorectal cancer (metastasis to ovary) shows moderate to strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12974-Immunohistochemistry-Paraffin-NBP3-18552-img0004.jpg)
![Immunohistochemistry-Paraffin: CDX2 Antibody (CL12974) [NBP3-18552] - Staining of human rectum shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12974-Immunohistochemistry-Paraffin-NBP3-18552-img0005.jpg)
![Immunohistochemistry-Paraffin: CDX2 Antibody (CL12974) [NBP3-18552] - Staining of human colon shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12974-Immunohistochemistry-Paraffin-NBP3-18552-img0006.jpg)
![Western Blot: Vimentin Antibody (CL0157) [NBP2-52867] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-52867) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Knockout-Validated-NBP2-52867-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody (CL0157) [NBP2-52867] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-52867) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: Vimentin Antibody (CL0157) [NBP2-52867] - Analysis in human cell lines U-251MG and MCF-7 using Anti-VIM antibody. Corresponding VIM RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Western-Blot-NBP2-52867-img0010.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human skin shows distinct positivity in fibroblasts.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Immunohistochemistry-Paraffin-NBP2-52867-img0005.jpg)
![Western Blot: Vimentin Antibody (CL0157) [NBP2-52867] - Lane 1: Marker [kDa] Lane 2: Human tonsil tissue lysate](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Western-Blot-NBP2-52867-img0006.jpg)
![Western Blot: Vimentin Antibody (CL0157) [NBP2-52867] - Lane 1: Marker [kDa], Lane 2: Human cell line HeLa cytoplasmic fraction , Lane 3: Human cell line HeLa membrane fraction , Lane 4: Human cell line HeLa nuclear fraction , Lane 5: Human cell line HeLa chromatin fraction , Lane 6: Human cell line HeLa cytos](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Western-Blot-NBP2-52867-img0007.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human cerebellum shows immunoreactivity in the endothelial cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Immunohistochemistry-Paraffin-NBP2-52867-img0001.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human kidney shows strong immunoreactivity in renal glomeruli but not tubuli cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Immunohistochemistry-Paraffin-NBP2-52867-img0002.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human prostate shows strong immunoreactivity in the connective tissue.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Immunohistochemistry-Paraffin-NBP2-52867-img0003.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human rectum shows strong positivity in mesenchymal and lymphoid cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-CL0157-Immunohistochemistry-Paraffin-NBP2-52867-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: CDX2 Antibody (CL12967) [NBP3-18571] - Analysis in human cell line CACO-2 and human cell line PC-3.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12967-Immunocytochemistry-Immunofluorescence-NBP3-18571-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CDX2 Antibody (CL12967) [NBP3-18571] - Analysis in human cell line CACO-2 and human cell line PC-3.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12967-Immunohistochemistry-Paraffin-NBP3-18571-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CDX2 Antibody (CL12967) [NBP3-18571] - Staining of CACO-2 cells using the Anti-CDX2 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule probes are visualized in red (where available).](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12967-Immunocytochemistry-Immunofluorescence-NBP3-18571-img0001.jpg)
![Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571] - Analysis in human colon and tonsil tissues using NBP3-18571 antibody. Corresponding CDX2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12967-Immunohistochemistry-Paraffin-NBP3-18571-img0002.jpg)
![Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571] - Staining of human colorectal cancer shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12967-Immunohistochemistry-Paraffin-NBP3-18571-img0003.jpg)
![Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571] - Staining of human colorectal cancer (metastasis to ovary) shows moderate to strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12967-Immunohistochemistry-Paraffin-NBP3-18571-img0004.jpg)
![Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571] - Staining of human colon shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12967-Immunohistochemistry-Paraffin-NBP3-18571-img0005.jpg)
![Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571] - Staining of human rectum shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12967-Immunohistochemistry-Paraffin-NBP3-18571-img0006.jpg)
![Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12967-Immunohistochemistry-Paraffin-NBP3-18571-img0007.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of CD16 in human peripheral blood with anti-CD16 (3G8) APC.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0014.jpg "Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of CD16 in human peripheral blood with anti-CD16 (3G8) APC.")
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) APC-CyTM7.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0001.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) PE.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0002.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) PE-CyTM7.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0003.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) PerCP.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0004.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) PerC-CyTM5.5](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0005.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) Pacific BlueTM.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0006.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) purified / GAM-APC.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0008.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of CD16 in human peripheral blood with anti-CD16 (3G8) Alexa Fluor® 647.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0009.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of CD16 in human peripheral blood with anti-CD16 (3G8) Pacific OrangeTM.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0010.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of CD16 in human peripheral blood with anti-CD16 (3G8) biotin.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0011.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of human peripheral blood with anti-CD16 (3G8) FITC.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0012.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of CD16 in human peripheral blood with anti-CD16 (3G8) Alexa Fluor® 488.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: EGFR Antibody (GFR450) [NBP2-29440] - Confocal Immunofluorescent analysis of A431 cells using AF488-labeled Isotype Control Monoclonal Antibody (IgG2a) (Green). DAPI was used to stain the cell nuclei (blue). (Negative Control).](http://images.novusbio.com/fullsize/EGFR-Antibody-GFR450-Immunocytochemistry-Immunofluorescence-NBP2-29440-img0006.jpg "Immunocytochemistry/Immunofluorescence: EGFR Antibody (GFR450) [NBP2-29440] - Confocal Immunofluorescent analysis of A431 cells using AF488-labeled Isotype Control Monoclonal Antibody (IgG2a) (Green). DAPI was used to stain the cell nuclei (blue). (Negative Control).")
![Immunohistochemistry-Paraffin: EGFR Antibody (GFR450) [NBP2-29440] - Formalin-fixed, paraffin-embedded squamous cell carcinoma stained with EGFR Ab (GFR450).](http://images.novusbio.com/fullsize/EGFR-Antibody-GFR450-Immunohistochemistry-Paraffin-NBP2-29440-img0003.jpg)
![Flow Cytometry: EGFR Antibody (GFR450) [NBP2-29440] - Flow Cytometry of human EGFR on A431 cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled EGFR antibody (GFR450).](http://images.novusbio.com/fullsize/EGFR-Antibody-GFR450-Flow-Cytometry-NBP2-29440-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: EGFR Antibody (GFR450) [NBP2-29440] - Confocal Immunofluorescent analysis of A431 cells using AF488-labeled EGFR Monoclonal Antibody (GFR450) (Green). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/EGFR-Antibody-GFR450-Immunocytochemistry-Immunofluorescence-NBP2-29440-img0005.jpg)
![Flow Cytometry: EGFR Antibody (GFR450) [NBP2-29440] - Flow Cytometry of human EGFR on A431 cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled EGFR Antibody (GFR450).](http://images.novusbio.com/fullsize/EGFR-Antibody-GFR450-Flow-Cytometry-NBP2-29440-img0008.jpg)
![Protein Array: EGFR Antibody (GFR450) [NBP2-29440] - Analysis of Protein Array containing more than 19,000 full-length human proteins using EGFR Antibody (GFR450). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/EGFR-Antibody-GFR450-Protein-Array-NBP2-29440-img0009.jpg)
![Western Blot: DLX3 Antibody [NBP2-24646] - Analysis of DLX3 in NIH 3T3 cell lysates in the 1) absence and 2) presence of immunizing peptide using NBP2-24646 at 3 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/DLX3-Antibody-Western-Blot-NBP2-24646-img0002.jpg "Western Blot: DLX3 Antibody [NBP2-24646] - Analysis of DLX3 in NIH 3T3 cell lysates in the 1) absence and 2) presence of immunizing peptide using NBP2-24646 at 3 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: DLX3 Antibody [NBP2-24646] - Analysis of DLX3 in formalin-fixed, paraffin-embedded mouse testis tissue using this antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/DLX3-Antibody-Immunohistochemistry-Paraffin-NBP2-24646-img0001.jpg)
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody [NBP1-84674] - Staining of human placenta shows weak to moderate cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-Immunohistochemistry-Paraffin-NBP1-84674-img0008.jpg "Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody [NBP1-84674] - Staining of human placenta shows weak to moderate cytoplasmic positivity in trophoblastic cells.")
![Immunohistochemistry: FoxO1/FKHR Antibody [NBP1-84674] - Staining of mouse cerebral cortex shows moderate to strong positivity in more than 75 % of neurons in the visual cortex.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-Immunohistochemistry-NBP1-84674-img0005.jpg)
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody [NBP1-84674] - Staining of human stomach shows weak to moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-Immunohistochemistry-Paraffin-NBP1-84674-img0006.jpg)
![Immunohistochemistry-Paraffin: Slit2 Antibody [NBP2-20398] - Human gastric carcinoma. SLIT2 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Slit2-Antibody-Immunohistochemistry-Paraffin-NBP2-20398-img0011.jpg "Immunohistochemistry-Paraffin: Slit2 Antibody [NBP2-20398] - Human gastric carcinoma. SLIT2 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - A431 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-HL114-Western-Blot-NBP3-13660-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - A431 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - Sodium Potassium ATPase Alpha 1 antibody [HL114] detects Sodium Potassium ATPase Alpha 1 protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: Sodium Potassium ATPase Alpha 1 stained by Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-HL114-Immunocytochemistry-Immunofluorescence-NBP3-13660-img0002.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - Sodium Potassium ATPase Alpha 1 antibody [HL114] detects Sodium Potassium ATPase Alpha 1 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Sodium Potassium ATPase Alpha 1 stained by Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-HL114-Immunohistochemistry-Paraffin-NBP3-13660-img0003.jpg)
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-HL114-Western-Blot-NBP3-13660-img0001.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - Sodium Potassium ATPase Alpha 1 antibody [HL114] detects Sodium Potassium ATPase Alpha 1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.Sodium Potassium ATPase Alpha 1 stained by Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13660_rabbit-sodium-potassium-atpase-alpha-1-mab-hl114-910202420131225.jpg)
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - Drosophila tissue extract (5 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13660_rabbit-sodium-potassium-atpase-alpha-1-mab-hl114-9102024205691.jpg)
![A431 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Sodium/Potassium ATPase alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13660_rabbit-sodium-potassium-atpase-alpha-1-mab-hl114-western-blot-261120251995423.jpg)
![Western Blot: EGFR Antibody [NB100-596] - NPM1 knockdown decreases EGF expression. NPM1 controls activation of the EGF/EGFR pathway downstream effectors. Proteins, extracted from shScr and shNPM1 LNCaP cells cultured in RPMI 1640 10%FBS, were electrophoresed by SDS-PAGE. Transferred membranes were immunoblotted with indicated antibodies. Histograms show the band quantification reported to the beta-actin level. Blots are representative of three independent experiments with consistent results. Data are representative of at least three independent experiments and are expressed as the mean +/- SD. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0096293), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/EGFR-Antibody-Western-Blot-NB100-596-img0016.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR Antibody [NB100-596] - NPM1 knockdown decreases EGF expression. NPM1 controls activation of the EGF/EGFR pathway downstream effectors. Proteins, extracted from shScr and shNPM1 LNCaP cells cultured in RPMI 1640 10%FBS, were electrophoresed by SDS-PAGE. Transferred membranes were immunoblotted with indicated antibodies. Histograms show the band quantification reported to the beta-actin level. Blots are representative of three independent experiments with consistent results. Data are representative of at least three independent experiments and are expressed as the mean +/- SD. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0096293), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: EGFR Antibody [NB100-596] - Section of human placenta. Antibody: Affinity purified rabbit anti-EGFR used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/EGFR-Antibody-Immunohistochemistry-Paraffin-NB100-596-img0013.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: EGFR Antibody [NB100-596] - EGF R/ErbB1 Antibody [NB100-596] - Whole cell lysate (50 ug) from 293T and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody:Affinity purified rabbit anti-EGFR antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/EGFR-Antibody-Western-Blot-NB100-596-img0015.jpg)
![Western Blot: EGFR Antibody [NB100-596] - Detection of Human EGFR on HeLa whole cell lysate using NB100-596.](http://images.novusbio.com/fullsize/EGFR-Antibody-Western-Blot-NB100-596-img0005.jpg)
![Western Blot: EGFR Antibody [NB100-596] - Detection of Human EGFR by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EGFR antibody NB100-596 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/EGFR-Antibody-Western-Blot-NB100-596-img0014.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: EGFR Antibody [NB100-596] - NPM1 knockdown in prostate cancer cells reduces proliferation and migration capacities by inhibiting the EGF/EGFR pathway activity.(a) NPM1 down-regulation inhibits EGF induced ERK1/2 pathway activity. shScr and shNPM1 LNCaP cells were treated with 100 nM EGF and phosphorylation of the EGF/EGFR pathway effectors was analyzed using Western Blotting. Histograms show the band quantification reported to the beta-actin level. The blot is representative of three independent experiments with consistent results. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0096293), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/EGFR-Antibody-Western-Blot-NB100-596-img0017.jpg)