Human Cortisol ELISA Kit (Colorimetric)

ELISA: Human Cortisol ELISA Kit (Colorimetric) [NBP3-18003] - Samples were spiked with high concentrations of Human Cortisol and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Cortisol ELISA Kit (Colorimetric) [NBP3-18003] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA, ELISA(Comp)
• Suitable Sample Type: Serum and Plasma
• Standard Curve Range: 6.25-400 ng/mL
• Sensitivity: 2.92 ng/mL

Human Cortisol ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 6.25-400 ng/mL
• Sensitivity: 2.92 ng/mL
• Assay Type: Competitive ELISA
• Inter-Assay: %CV < 8.96
• Intra-Assay: %CV < 8.78
• Spike Recovery: 81-100%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • Competitive ELISA
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Cortisol ELISA Kit (Colorimetric)

• Compound F
• Cortisol
• Hydrocortisone

Background

Cortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). ACTH and CRF secretions are inhibited by high cortisol levels in a negative feedback loop. In plasma a majority of cortisol is bound with high affinity to corticosteroid binding globulin (CBG or transcotin). Cortisol acts through specific intracellular receptors and affects numerous physiologic systems including immune function, glucose counter regulation, vascular tone, and bone metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Cortisol ELISA Kit (NBP3-18003)(1)

Publication using NBP3-18003

• Calapai M, Puzzo L, Bova G et al. Effects of Physical Exercise and Motor Activity on Depression and Anxiety in Post?Mastectomy Pain Syndrome preprints.org 2023-11-23 [PMID: 38255692] (ELISA)

FAQs for Cortisol ELISA Kit (NBP3-18003). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.