| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08936 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70146 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76480-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73822 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70623 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77113-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95105-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81016 | |||
| BSA Free | NB100-406SS | |||
![Western Blot: Jagged 1 Antibody (1E12.) [H00000182-M01A] - Western Blot detection against Immunogen (35.64 KDa) .](http://images.novusbio.com/fullsize/Jagged-1-Antibody-1E12-Western-Blot-H00000182-M01A-img0001.jpg "Western Blot: Jagged 1 Antibody (1E12.) [H00000182-M01A] - Western Blot detection against Immunogen (35.64 KDa) .")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: Jagged 1 Antibody (1E12) [H00000182-M01A] - Jagged 1 regulates learning-dependent Notch induction and is enriched presynaptically. Fluorescent double-labeling shows the expression of Notch1 (red) following spatial exploration in CTLs and Jagged 1cKO. Jagged 1 labeling (green) is used to validate the absence of Jagged 1 in the Jagged 1cKO (Jag1cKOs). Image collected and cropped by CiteAb from the following publication (www.journal.frontiersin.org/article/10.3389/fncel.2017.00220/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Jagged-1-Antibody-1E12-Knockout-Validated-H00000182-M01A-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Jagged 1 Antibody (1E12) [H00000182-M01A] - Targeted loss of Jagged1 in adult mouse neurons causes a spatial memory deficit. A schematic representation of the Jagged1 floxed allele used to generate the mice with TAM-inducible loss of Jagged1 gene (A). Representative immunofluorescence images of single confocal z-plane showing a near complete loss of Jagged1 protein (green) from hippocampal pyramidal neurons labeled by NeuN (red) of Jagged1cKO mice (B). Representative images from immunoblots showing the expression of Jagged1, DNER in Jagged1cKO mice & control mice (C). GAPDH is used as a loading control. Bar graph showing the quantitation of optical densities of Jagged1 & DNER bands in CTLs & Jagged1cKO mice (D). A graphic showing the behavioral arena for Y-maze spontaneous alternation test (E). Jagged1cKO mice show a significant deficit in hippocampus dependent working memory in the Y-maze spontaneous alternation test (F). A diagram showing the experimental arena for the hidden arm version of the Y-maze (G). Jagged1cKO mice show a significant reduction in the time spent in the hidden arm, suggesting a spatial memory defect (H). A schematic showing the experimental setup for the Novel Object Displacement test (I). Jagged1cKO mice exhibit a statistical significant reduction in discrimination index, a measure of spatial memory defect (J). Scale bar in (B) is 50 μm. Graphs are represented as mean ± SEM, *p < 0.05, **p < 0.01, & ***p < 0.001. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fncel.2017.00220/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00000182-m01a_mouse-monoclonal-jagged-1-antibody-1e12-310202415485115.jpg)
![Immunocytochemistry/ Immunofluorescence: Jagged 1 Antibody (1E12) [H00000182-M01A] - Jagged1 regulates learning-dependent Notch induction & is enriched presynaptically. Fluorescent double-labeling shows the expression of Notch1 (red) following spatial exploration in CTLs & Jagged1cKO (A). Jagged1 labeling (green) is used to validate the absence of Jagged1 in the Jagged1cKO (Jag1cKOs) (A,C). Diagram summarizing the Notch1 fluorescence intensities distribution in randomly picked 69 & 71 CA1 neurons in CTLs & Jagged1cKOs, respectively (p < 0.001) (B). Double immunofluorescence of the Notch1 transcriptional target, Hes5, in CTLs & JaggedcKOs following spatial exploration (C). Diagram summarizing the Hes5 fluorescence intensity distribution in randomly picked 64 & 70 CA1 neurons in CTL & Jagged1cKO, respectively (p < 0.001) (D). Representatives gold Immuno-electron microscopy panels from three WT mice show that Jagged1 particles are localized in presynaptic terminals, bound to presynaptic vesicles (Pre) (E–E‴). Immunogold particles are also apparent at the Postsynaptic density (Post) (E,E″,E‴). Box Plot summarizing the particle counts in presynaptic & postsynaptic terminal areas drawn on Immunoelectromicroscopy micrograph for Jagged1 indicates that the presynapse is enriched with Jag1 particles as compared to the postsynaptic terminal (p < 0.001) (F). Scale bars are: 20 μm in (A), 10 μm in (C), 400 nm in (E–E‴). AU, arbitrary units; Pre, presynaptic; Post, postsynaptic; & Ax, axon. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fncel.2017.00220/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00000182-m01a_mouse-monoclonal-jagged-1-antibody-1e12-310202415483644.jpg)
![Immunocytochemistry/ Immunofluorescence: Jagged 1 Antibody (1E12) [H00000182-M01A] - Jagged1 expression in brains & CSF of AD patients. Representative double fluorescent immunolabelings for Jagged1 (green) & Notch1 (red) counterstained with Thioflavin-T or DAPI (blue) on postmortem brain sections comprising the hippocampal CA fields from healthy age-matched controls & AD patients (A–C). in healthy controls, Jagged1 is localized to somata of neurons where also Notch1 is expressed. As expected, Thioflavin-T labeling is negligible (A,A′). AD sections show fibrillary aggregates (B,B′) & (C,C′) core plaques double positive for Notch1 & Thioflavin-T. Jag1 expression is scattered in parenchyma & low in, degenerated neurons (white arrows) (B,B′). Jag1 overlays in small double positive aggregates for Notch1 & Thioflavin-T (100x magnification) in radiating plaques with a visible reduction in Jagged1 cellular expression (B–C′). Box plots summarizing the quantification of fluorescence intensities of Jagged1 immunolabeled neurons shows a significant reduction in Jagged1 expression in AD patients (p < 0.001). Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fncel.2017.00220/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00000182-m01a_mouse-monoclonal-jagged-1-antibody-1e12-310202415485139.jpg)
![Immunocytochemistry/ Immunofluorescence: Jagged 1 Antibody (1E12) [H00000182-M01A] - Jagged1 expression in brains & CSF of AD patients. Representative double fluorescent immunolabelings for Jagged1 (green) & Notch1 (red) counterstained with Thioflavin-T or DAPI (blue) on postmortem brain sections comprising the hippocampal CA fields from healthy age-matched controls & AD patients (A–C). in healthy controls, Jagged1 is localized to somata of neurons where also Notch1 is expressed. As expected, Thioflavin-T labeling is negligible (A,A′). AD sections show fibrillary aggregates (B,B′) & (C,C′) core plaques double positive for Notch1 & Thioflavin-T. Jag1 expression is scattered in parenchyma & low in, degenerated neurons (white arrows) (B,B′). Jag1 overlays in small double positive aggregates for Notch1 & Thioflavin-T (100x magnification) in radiating plaques with a visible reduction in Jagged1 cellular expression (B–C′). Box plots summarizing the quantification of fluorescence intensities of Jagged1 immunolabeled neurons shows a significant reduction in Jagged1 expression in AD patients (p < 0.001). Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fncel.2017.00220/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00000182-m01a_mouse-monoclonal-jagged-1-antibody-1e12-310202415482294.jpg)
![Western Blot: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Western Blot Analysis of human U-87 cell lysate using Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Western-Blot-NBP3-07191-img0005.jpg "Western Blot: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Western Blot Analysis of human U-87 cell lysate using Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).")
![Immunohistochemistry-Paraffin: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Formalin-fixed, paraffin-embedded human tonsil stained with Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Immunohistochemistry-Paraffin-NBP3-07191-img0003.jpg)
![Western Blot: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Western Blot Analysis of human A375 cell lysate using Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Western-Blot-NBP3-07191-img0004.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Formalin-fixed, paraffin-embedded human melanoma stained with Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Immunohistochemistry-Paraffin-NBP3-07191-img0001.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Immunohistochemistry-Paraffin-NBP3-07191-img0002.jpg)
![Western Blot: AKT2 Antibody (4H7) [NBP2-02231] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT2.](http://images.novusbio.com/fullsize/AKT2-Antibody-4H7-Western-Blot-NBP2-02231-img0003.jpg "Western Blot: AKT2 Antibody (4H7) [NBP2-02231] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT2.")
![Immunocytochemistry/Immunofluorescence: AKT2 Antibody (4H7) [NBP2-02231] Staining of COS7 cells transiently transfected by pCMV6-ENTRY AKT2.](http://images.novusbio.com/fullsize/AKT2-Antibody-4H7-Immunocytochemistry-Immunofluorescence-NBP2-02231-img0007.jpg)
![Immunohistochemistry-Paraffin: AKT2 Antibody (4H7) [NBP2-02231] - Staining of paraffin-embedded Human liver tissue using anti-AKT2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AKT2-Antibody-4H7-Immunohistochemistry-Paraffin-NBP2-02231-img0006.jpg)
![Flow Cytometry: AKT2 Antibody (4H7) [NBP2-02231] - Analysis of Jurkat cells, using anti-AKT2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/AKT2-Antibody-4H7-Flow-Cytometry-NBP2-02231-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: AKT2 Antibody (4H7) [NBP2-02231] - Immunofluorescent staining of HepG2 cells using anti-AKT2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AKT2-Antibody-4H7-Immunocytochemistry-Immunofluorescence-NBP2-02231-img0004.jpg)
![Immunohistochemistry-Paraffin: AKT2 Antibody (4H7) [NBP2-02231] - Staining of paraffin-embedded Human Kidney tissue using anti-AKT2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AKT2-Antibody-4H7-Immunohistochemistry-Paraffin-NBP2-02231-img0005.jpg)
![Flow Cytometry: AKT2 Antibody (4H7) [NBP2-02231] - Analysis of Hela cells, using anti-AKT2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/AKT2-Antibody-4H7-Flow-Cytometry-NBP2-02231-img0001.jpg)
![Western Blot: CXCR4 Antibody [NBP1-76480] - Independent Antibody Validation (IAV) via Protein Expression Profile Loading: 15 ug of lysates per lane. Antibodies: NBP1-77067 (1 ug/mL), NBP1-76480 (1 ug/mL), and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-76480-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: CXCR4 Antibody [NBP1-76480] - Independent Antibody Validation (IAV) via Protein Expression Profile Loading: 15 ug of lysates per lane. Antibodies: NBP1-77067 (1 ug/mL), NBP1-76480 (1 ug/mL), and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST.")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody - BSA Free [NBP1-76480] - Figure 5 Immunofluorescence Validation of CXCR4 Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling CXCR4 with at 4 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on HeLa cell line.](http://images.novusbio.com/fullsize/nbp1-76480_rabbit-polyclonal-cxcr4-antibody-2711202419562119.jpg)
![Immunohistochemistry-Paraffin: CXCR4 Antibody [NBP1-76480] - Staining of CXCR4 in mouse spleen tissue with CXCR4 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Immunohistochemistry-Paraffin-NBP1-76480-img0006.jpg)
![Western Blot: CXCR4 Antibody [NBP1-76480] - HeLa total cell lysate.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-76480-img0002.jpg)
![Western Blot: CXCR4 Antibody [NBP1-76480] - Animal Species ReactivityLoading: Lysates/proteins at 20 ug per lane.Antibodies: NBP1-77067 (2 ug/mL) or NBP1-76480 (2 ug/mL). 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-76480-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CXCR4 Antibody [NBP1-76480] - Validation with CXCR4 siRNA Knockdown. HeLa cells were transfected with control siRNAs (lane 1) or CXCR4 siRNAs (lane 2) Loading: 10 ug of HeLa whole cell lysates per lane. Antibodies: NBP1-76480 (2 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Knockdown-Validated-NBP1-76480-img0008.jpg)
![Western Blot: RbAp46 Antibody (5A4) [NBP2-01308] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RbAp46 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RbAp46.](http://images.novusbio.com/fullsize/RbAp46-Antibody-5A4-Western-Blot-NBP2-01308-img0001.jpg "Western Blot: RbAp46 Antibody (5A4) [NBP2-01308] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RbAp46 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RbAp46.")
![Immunohistochemistry-Paraffin: RbAp46 Antibody (5A4) [NBP2-01308] Staining of paraffin-embedded Human prostate tissue using anti-RbAp46 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RbAp46-Antibody-5A4-Immunohistochemistry-Paraffin-NBP2-01308-img0009.jpg)
![Immunohistochemistry-Paraffin: RbAp46 Antibody (5A4) [NBP2-01308] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-RbAp46 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RbAp46-Antibody-5A4-Immunohistochemistry-Paraffin-NBP2-01308-img0002.jpg)
![Immunohistochemistry-Paraffin: RbAp46 Antibody (5A4) [NBP2-01308] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RbAp46 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RbAp46-Antibody-5A4-Immunohistochemistry-Paraffin-NBP2-01308-img0003.jpg)
![Immunohistochemistry-Paraffin: RbAp46 Antibody (5A4) [NBP2-01308] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-RbAp46 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RbAp46-Antibody-5A4-Immunohistochemistry-Paraffin-NBP2-01308-img0004.jpg)
![Immunohistochemistry-Paraffin: RbAp46 Antibody (5A4) [NBP2-01308] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-RbAp46 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RbAp46-Antibody-5A4-Immunohistochemistry-Paraffin-NBP2-01308-img0005.jpg)
![Immunohistochemistry-Paraffin: RbAp46 Antibody (5A4) [NBP2-01308] - Staining of paraffin-embedded Human endometrium tissue using anti-RbAp46 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RbAp46-Antibody-5A4-Immunohistochemistry-Paraffin-NBP2-01308-img0006.jpg)
![Immunohistochemistry-Paraffin: RbAp46 Antibody (5A4) [NBP2-01308] - Staining of paraffin-embedded Human Kidney tissue using anti-RbAp46 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RbAp46-Antibody-5A4-Immunohistochemistry-Paraffin-NBP2-01308-img0007.jpg)
![Immunohistochemistry-Paraffin: RbAp46 Antibody (5A4) [NBP2-01308] - Staining of paraffin-embedded Human pancreas tissue using anti-RbAp46 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RbAp46-Antibody-5A4-Immunohistochemistry-Paraffin-NBP2-01308-img0008.jpg)
![Immunohistochemistry: Ephrin-A3 Antibody (4A4) [NBP2-46484] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Ephrin-A3-Antibody-4A4-Immunohistochemistry-NBP2-46484-img0006.jpg "Immunohistochemistry: Ephrin-A3 Antibody (4A4) [NBP2-46484] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)")
![Immunohistochemistry: Ephrin-A3 Antibody (4A4) [NBP2-46484] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Ephrin-A3-Antibody-4A4-Immunohistochemistry-NBP2-46484-img0001.jpg)
![Immunohistochemistry: Ephrin-A3 Antibody (4A4) [NBP2-46484] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Ephrin-A3-Antibody-4A4-Immunohistochemistry-NBP2-46484-img0002.jpg)
![Immunohistochemistry: Ephrin-A3 Antibody (4A4) [NBP2-46484] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Ephrin-A3-Antibody-4A4-Immunohistochemistry-NBP2-46484-img0003.jpg)
![Immunohistochemistry: Ephrin-A3 Antibody (4A4) [NBP2-46484] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Ephrin-A3-Antibody-4A4-Immunohistochemistry-NBP2-46484-img0004.jpg)
![Immunohistochemistry: Ephrin-A3 Antibody (4A4) [NBP2-46484] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Ephrin-A3-Antibody-4A4-Immunohistochemistry-NBP2-46484-img0005.jpg)
![Immunohistochemistry: Ephrin-A3 Antibody (4A4) [NBP2-46484] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Ephrin-A3-Antibody-4A4-Immunohistochemistry-NBP2-46484-img0007.jpg)
![Immunohistochemistry-Paraffin: Ephrin-A3 Antibody (OTI4A4) [NBP2-46484] - Ephrin-A3 immunoreactivity in mouse trigeminal ganglion neurons. Sections were incubated with the antibody overnight at 4 degrees. Biotinylated mouse IgG was used to help verify the specificity of the antibody (NBP1-97078). Biotin conjugated version of antibody used (NBP2-70623B). Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-46484_mouse-ephrin-a3-mab-oti4a4-1772024104468.jpg)
![Western Blot: CDX2 Antibody [NBP1-77113] - Mouse brain tissue lysate at 1 ug/mL.](http://images.novusbio.com/fullsize/CDX2-Antibody-Western-Blot-NBP1-77113-img0004.jpg "Western Blot: CDX2 Antibody [NBP1-77113] - Mouse brain tissue lysate at 1 ug/mL.")
![Immunocytochemistry/Immunofluorescence: CDX2 Antibody [NBP1-77113] - Immunofluorescence of CDX2 in rat brain tissue with CDX2 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/CDX2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77113-img0003.jpg)
![Immunohistochemistry-Paraffin: CDX2 Antibody [NBP1-77113] - Rat brain tissue with CDX2 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/CDX2-Antibody-Immunohistochemistry-Paraffin-NBP1-77113-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: CDX2 Antibody - BSA Free [NBP1-77113] - Immunofluorescence of CDX2 in rat brain tissue with CDX2 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77113_rabbit-polyclonal-cdx2-antibody-2711202420243356.jpg)
![Immunohistochemistry: CDX2 Antibody - BSA Free [NBP1-77113] - Immunohistochemistry of CDX2 in rat brain tissue with CDX2 antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp1-77113_rabbit-polyclonal-cdx2-antibody-2711202420195354.jpg)
![Immunocytochemistry/ Immunofluorescence: CDX2 Antibody - BSA Free [NBP1-77113] - Immunofluorescence of CDX2 in rat brain tissue with CDX2 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77113_rabbit-polyclonal-cdx2-antibody-2711202420414545.jpg)
![Immunohistochemistry: CDX2 Antibody - BSA Free [NBP1-77113] - Immunohistochemistry of CDX2 in rat brain tissue with CDX2 antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp1-77113_rabbit-polyclonal-cdx2-antibody-27112024201315.jpg)
![Western Blot: Notch-1 Antibody [NBP2-95105] - Analysis of extracts of U-251MG cells, using Notch-1 .Exposure time: 300s.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Western-Blot-NBP2-95105-img0006.jpg "Western Blot: Notch-1 Antibody [NBP2-95105] - Analysis of extracts of U-251MG cells, using Notch-1 .Exposure time: 300s.")
![Immunohistochemistry: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using Notch-1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-5220251638038.jpg)
![Immunohistochemistry: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using Notch-1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-522025163964.jpg)
![Immunohistochemistry: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Notch-1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-5220251638034.jpg)
![Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunofluorescence analysis of U2OS cells using Notch-1 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-5220251639698.jpg)
![Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunofluorescence analysis of HeLa cells using Notch-1 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-5220251636390.jpg)
![Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunofluorescence analysis of A-549 cells using Notch-1 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-52202516283218.jpg)
![Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunofluorescence analysis of PC-12 cells using Notch-1 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-5220251638067.jpg)
![Immunohistochemistry: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Notch-1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-522025163633.jpg)
![Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody - Azide and BSA Free [Notch-1] - Immunofluorescence analysis of NIH/3T3 cells using Notch-1 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95105_rabbit-polyclonal-notch-1-antibody-52202516345270.jpg)
![Western Blot: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Western blot analysis of extracts of various cell lines, using RBBP4/RbAp48 Rabbit mAb (NBP3-16237) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-0D1X1-Western-Blot-NBP3-16237-img0007.jpg "Western Blot: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Western blot analysis of extracts of various cell lines, using RBBP4/RbAp48 Rabbit mAb (NBP3-16237) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunocytochemistry/ Immunofluorescence: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Confocal imaging of NIH/3T3 cells using RBBP4/RbAp48 Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16237_rabbit-rbbp4-rbap48-mab-0d1x1-52202511411412.jpg)
![Immunocytochemistry/ Immunofluorescence: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Confocal imaging of U-2 OS cells using RBBP4/RbAp48 Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16237_rabbit-rbbp4-rbap48-mab-0d1x1-52202512548.jpg)
![Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Immunohistochemistry of paraffin-embedded mouse brain using RBBP4/RbAp48 Rabbit mAb (NBP3-16237) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-0D1X1-Immunohistochemistry-Paraffin-NBP3-16237-img0003.jpg)
![Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Immunohistochemistry of paraffin-embedded rat brain using RBBP4/RbAp48 Rabbit mAb (NBP3-16237) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-0D1X1-Immunohistochemistry-Paraffin-NBP3-16237-img0001.jpg)
![Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Immunohistochemistry of paraffin-embedded human lung cancer using RBBP4/RbAp48 Rabbit mAb (NBP3-16237) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-0D1X1-Immunohistochemistry-Paraffin-NBP3-16237-img0002.jpg)
![Immunohistochemistry: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Immunohistochemistry analysis of paraffin-embedded human breast cancer using RBBP4/RbAp48 Rabbit mAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16237_rabbit-rbbp4-rbap48-mab-0d1x1-522025125555.jpg)
![Immunohistochemistry: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Immunohistochemistry analysis of paraffin-embedded human tonsil using RBBP4/RbAp48 Rabbit mAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16237_rabbit-rbbp4-rbap48-mab-0d1x1-52202512543.jpg)
![Immunohistochemistry: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using RBBP4/RbAp48 Rabbit mAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16237_rabbit-rbbp4-rbap48-mab-0d1x1-5220251215417.jpg)
![Immunohistochemistry: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Immunohistochemistry analysis of paraffin-embedded rat kidney using RBBP4/RbAp48 Rabbit mAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16237_rabbit-rbbp4-rbap48-mab-0d1x1-522025121540.jpg)
![Immunohistochemistry: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using RBBP4/RbAp48 Rabbit mAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16237_rabbit-rbbp4-rbap48-mab-0d1x1-5220251242580.jpg)
![Immunohistochemistry: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Immunohistochemistry analysis of paraffin-embedded human placenta using RBBP4/RbAp48 Rabbit mAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16237_rabbit-rbbp4-rbap48-mab-0d1x1-52202511392291.jpg)
![Immunohistochemistry: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Immunohistochemistry analysis of paraffin-embedded human kidney using RBBP4/RbAp48 Rabbit mAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16237_rabbit-rbbp4-rbap48-mab-0d1x1-52202511383781.jpg)
![Immunohistochemistry: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Immunohistochemistry analysis of paraffin-embedded human colon using RBBP4/RbAp48 Rabbit mAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16237_rabbit-rbbp4-rbap48-mab-0d1x1-5220251141143.jpg)
![Immunohistochemistry: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Immunohistochemistry analysis of paraffin-embedded human liver using RBBP4/RbAp48 Rabbit mAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16237_rabbit-rbbp4-rbap48-mab-0d1x1-5220251242517.jpg)
![Immunohistochemistry: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Immunohistochemistry analysis of paraffin-embedded mouse liver using RBBP4/RbAp48 Rabbit mAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16237_rabbit-rbbp4-rbap48-mab-0d1x1-52202511402783.jpg)
![Immunohistochemistry: RBBP4/RbAp48 Antibody (0D1X1) [NBP3-16237] - Immunohistochemistry analysis of paraffin-embedded mouse intestin using RBBP4/RbAp48 Rabbit mAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16237_rabbit-rbbp4-rbap48-mab-0d1x1-52202511374173.jpg)
![Western Blot: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of PEN2 on mouse spleen tissue lysate using anti-PEN2 antibody at 1/2,000 dilution.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Western-Blot-NBP2-75632-img0009.jpg "Western Blot: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of PEN2 on mouse spleen tissue lysate using anti-PEN2 antibody at 1/2,000 dilution.")
![Immunocytochemistry/Immunofluorescence: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Staining PEN2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunocytochemistry-Immunofluorescence-NBP2-75632-img0003.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded rat cerebellum tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0008.jpg)
![Flow Cytometry: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of A549 cells with PEN2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Flow-Cytometry-NBP2-75632-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Staining PEN2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunocytochemistry-Immunofluorescence-NBP2-75632-img0002.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded human lung cancer tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0004.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded human kidney tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0005.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded human tonsil tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0006.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded mouse testis tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0007.jpg)
![Immunoprecipitation: Vimentin Antibody (SC60-05) [NBP3-33094] - Vimentin was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-33094 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-33094 at 1/10,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-33094 in HeLa cell lysate Lane 3: NBP3-33094 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 5 seconds](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-2682024158265.jpg "Immunoprecipitation: Vimentin Antibody (SC60-05) [NBP3-33094] - Vimentin was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-33094 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-33094 at 1/10,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-33094 in HeLa cell lysate Lane 3: NBP3-33094 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 5 seconds")
![Immunohistochemistry-Frozen: Vimentin Antibody (SC60-05) [NBP3-33094] - Immunofluorescence analysis of frozen mouse hippocampus tissue labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33094).The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33094, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-2682024159390.jpg)
![Immunohistochemistry: Vimentin Antibody (SC60-05) [NBP3-33094] - Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma tissue with Rabbit anti-Vimentin antibody (NBP3-33094) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33094) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-298202414161822.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody (SC60-05) [NBP3-33094] - Immunocytochemistry analysis of C6 cells labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33094) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-29202410234261.jpg)
![Flow Cytometry: Vimentin Antibody (SC60-05) [NBP3-33094] - Flow cytometric analysis of HeLa (positive, red) and MCF7 (negative, green) cells labeling Vimentin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33094) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (HeLa black, MCF7 light green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-29202416453228.jpg)
![Western Blot: Vimentin Antibody (SC60-05) [NBP3-33094] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-33094) at 1/20,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: HEK-293 cell lysate (10 µg/Lane) Lane 3: Jurkat cell lysate (10 µg/Lane) Lane 4: C2C12 cell lysate (10 µg/Lane) Lane 5: RAW264.7 cell lysate (10 µg/Lane) Lane 6: C6 cell lysate (10 µg/Lane) Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-3920248453527.jpg)
![Western Blot: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - (1) HeLa, (2) PC12 and (3) NIH-3T3 whole cell lysates were separated by SDS-PAGE on a 12% gel and transferred to PVDF membrane. The membrane was probed with Anti-14-3-3 gamma [Ac Val2] antibody at 2 ug/ml for 1 hour, followed by incubation a 1:10,000 dilution of anti-rabbit HRP. The protein was detected at approximately 28 kDa after incubation with SuperSignal West Pico Chemiluminescent substrate.](http://images.novusbio.com/fullsize/14-3-3-gamma-ac-Val2-Antibody-KC21-Western-Blot-NB100-406-img0005.jpg "Western Blot: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - (1) HeLa, (2) PC12 and (3) NIH-3T3 whole cell lysates were separated by SDS-PAGE on a 12% gel and transferred to PVDF membrane. The membrane was probed with Anti-14-3-3 gamma [Ac Val2] antibody at 2 ug/ml for 1 hour, followed by incubation a 1:10,000 dilution of anti-rabbit HRP. The protein was detected at approximately 28 kDa after incubation with SuperSignal West Pico Chemiluminescent substrate.")
![Immunocytochemistry/Immunofluorescence: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - 14-3-3 gamma [ac Val2] antibody was tested in SH-SY5Y cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/14-3-3-gamma-ac-Val2-Antibody-KC21-Immunocytochemistry-Immunofluorescence-NB100-406-img0004.jpg)
![Western Blot: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - 14-3-3 gamma detected in lysates using NB100-406. Lane 1: HeLa cell lysates, Lanes 2 and 3: bengamide treated lysates (8h and 24h, respectively).](http://images.novusbio.com/fullsize/14-3-3-gamma-ac-Val2-Antibody-KC21-Western-Blot-NB100-406-img0002.jpg)
![Simple Western: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - Lane view shows a specific band for 14-3-3 Gamma in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/14-3-3-gamma-ac-Val2-Antibody-KC21-Simple-Western-NB100-406-img0007.jpg)
