| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88774-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72088 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25150SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB100-98931-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97472-0.2mg | |||
| BSA Free | NBP1-97472-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77169-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92868-0.02ml | |||
![Western Blot: GOLM1 Antibody [NBP1-88774] - Analysis in control (vector only transfected HEK293T lysate) and GOLM1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/GOLM1-Antibody-Western-Blot-NBP1-88774-img0010.jpg "Western Blot: GOLM1 Antibody [NBP1-88774] - Analysis in control (vector only transfected HEK293T lysate) and GOLM1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: GOLM1 Antibody [NBP1-88774] - Staining of human cell line U-251 MG shows localization to the Golgi apparatus.](http://images.novusbio.com/fullsize/GOLM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88774-img0009.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GOLM1 Antibody [NBP1-88774] - Staining in human stomach and skeletal muscle tissues using anti-GOLM1 antibody. Corresponding GOLM1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GOLM1-Antibody-Immunohistochemistry-Paraffin-NBP1-88774-img0008.jpg)
![Western Blot: GOLM1 Antibody [NBP1-88774] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/GOLM1-Antibody-Western-Blot-NBP1-88774-img0006.jpg)
![Immunohistochemistry-Paraffin: GOLM1 Antibody [NBP1-88774] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/GOLM1-Antibody-Immunohistochemistry-Paraffin-NBP1-88774-img0005.jpg)
![Immunohistochemistry-Paraffin: GOLM1 Antibody [NBP1-88774] - Staining of human stomach shows high expression.](http://images.novusbio.com/fullsize/GOLM1-Antibody-Immunohistochemistry-Paraffin-NBP1-88774-img0007.jpg)
![Western Blot: COG6 Antibody (OTI1B8) [NBP2-45631] - Analysis of extracts (35ug) from 9 different cell lines. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/COG6-Antibody-OTI1B8-Western-Blot-NBP2-45631-img0004.jpg "Western Blot: COG6 Antibody (OTI1B8) [NBP2-45631] - Analysis of extracts (35ug) from 9 different cell lines. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunohistochemistry: COG6 Antibody (OTI1B8) [NBP2-45631] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/COG6-Antibody-OTI1B8-Immunohistochemistry-NBP2-45631-img0003.jpg)
![Western Blot: COG6 Antibody (OTI1B8) [NBP2-45631] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY COG6.](http://images.novusbio.com/fullsize/COG6-Antibody-OTI1B8-Western-Blot-NBP2-45631-img0005.jpg)
![Immunohistochemistry: COG6 Antibody (OTI1B8) [NBP2-45631] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/COG6-Antibody-OTI1B8-Immunohistochemistry-NBP2-45631-img0002.jpg)
![Immunofluorescence: COG6 Antibody (OTI1B8) [NBP2-45631] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY COG6.](http://images.novusbio.com/fullsize/COG6-Antibody-OTI1B8-Immunofluorescence-NBP2-45631-img0001.jpg)
![Western Blot: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody (MEM-238) [NB500-464] - Fig. 1. Western Blotting analysis (non-reducing conditions) of CD222 in whole cell lysate of JURKAT human peripheral blood T cell leukemia cell line. Lane 1: immunostaining with anti-CD222 (MEM-238) Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06).](http://images.novusbio.com/fullsize/IGF-II-R-Mannose-6-Phosphate-Receptor-Cation-independent-Antibody-MEM-238-Western-Blot-NB500-464-img0001.jpg "Western Blot: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody (MEM-238) [NB500-464] - Fig. 1. Western Blotting analysis (non-reducing conditions) of CD222 in whole cell lysate of JURKAT human peripheral blood T cell leukemia cell line. Lane 1: immunostaining with anti-CD222 (MEM-238) Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06).")
![Flow Cytometry: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody (MEM-238) [NB500-464] - Flow Cytometry: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody (MEM-238) [FITC] [NB100-63599] - Mannose 6 Phosphate Receptor (Cation independent) Antibody (MEM-238)[NB100-63599]- Staining human peripheral blood monocytes following permeablisation with Leucoperm (Fix & Perm) (BUF09). Image using the FITC form of this antibody.](http://images.novusbio.com/fullsize/IGF-II-R-Mannose-6-Phosphate-Receptor-Cation-independent-Antibody-MEM-238-Flow-Cytometry-NB500-464-img0004.jpg)
![Flow Cytometry: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody (MEM-238) [NB500-464] - Intracellular staining of human peripheral blood with anti-CD222 (MEM-238) PE.](http://images.novusbio.com/fullsize/IGF-II-R-Mannose-6-Phosphate-Receptor-Cation-independent-Antibody-MEM-238-Flow-Cytometry-NB500-464-img0002.jpg)
![Flow Cytometry: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody (MEM-238) [NB500-464] - Surface staining of human peripheral blood with anti-CD222 (MEM-238) biotin, streptavidin-APC](http://images.novusbio.com/fullsize/IGF-II-R-Mannose-6-Phosphate-Receptor-Cation-independent-Antibody-MEM-238-Flow-Cytometry-NB500-464-img0003.jpg)
![Western Blot: HSP27 Antibody [NBP2-25150] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2000 of Chicken Anti-Human HSP29 Polyclonal Antibody (Catalog # NBP2-25150) followed by HRP-conjugated Anti-Chicken Secondary Antibody. Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-Knockout-Validated-NBP2-25150-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-25150] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2000 of Chicken Anti-Human HSP29 Polyclonal Antibody (Catalog # NBP2-25150) followed by HRP-conjugated Anti-Chicken Secondary Antibody. Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-25150] - Analysis of whole cell lysates using chicken pAb to HSP27, NBP2-25150, dilution 1:2,000, in red. [1] protein standard (red), [2] NIH-3T3 , [3] C6, [4] HeLa, [5] HEK293, [6] SH-SY5Y, and [7] COS-1 cells. Strong single band at 27kDa corresponds to the HSP27 protein strongly expressed in human cell lines. Note that the antibody does not recognize rodent HSP27. The blot was simultaneously probed with mouse mAb to Beta-tubulin, dilution 1:10,000 in green. A single band at 50kDa, which is detected in all cell lysates, corresponds to the Beta-tubulin proteins.](http://images.novusbio.com/fullsize/HSP27-Antibody-NBP2-25150-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NBP2-25150] - Staining of HeLa cells stained with chicken pAb to heat shock protein 27 (HSP27), NBP2-25150, dilution 1:1,000 in green, and costained with mouse mAb to HSP60, dilution 1:5,000 in red. Blue is DAPI staining of nuclear DNA. NBP2-25150 antibody produces strong cytoplasmic staining, while the HSP60 antibody specifically labels mitochondria.](http://images.novusbio.com/fullsize/HSP27-Antibody-NBP2-25150-img0001.jpg)
![Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-98931] - IHC-P on paraffin sections of rat DRG.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer from manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/VPS45-Antibody-Immunohistochemistry-Paraffin-NB100-98931-img0029.jpg "Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-98931] - IHC-P on paraffin sections of rat DRG.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer from manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.")
![Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-98931] - Rat spinal cord using Rabbit antibody to internal part of Vacuolar protein sorting-associated protein 45 (rvps45, Vps45, Vps45a): IgG at a concentration of 10 ug/ml.](http://images.novusbio.com/fullsize/VPS45-Antibody-Immunohistochemistry-Paraffin-NB100-98931-img0016.jpg)
![Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-98931] - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. DAB chromogen. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/VPS45-Antibody-Immunohistochemistry-Paraffin-NB100-98931-img0018.jpg)
![Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-98931] - IHC-P on paraffin sections of rat brain.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer following manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/VPS45-Antibody-Immunohistochemistry-Paraffin-NB100-98931-img0021.jpg)
![Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-98931] - IHC-P on paraffin sections of rat brain.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer from manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/VPS45-Antibody-Immunohistochemistry-Paraffin-NB100-98931-img0027.jpg)
![Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-98931] - IHC-P on paraffin sections of rat DRG.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer from manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/VPS45-Antibody-Immunohistochemistry-Paraffin-NB100-98931-img0030.jpg)
![Immunohistochemistry-Paraffin: Furin Antibody [NBP2-24687] - analysis of Furin in formalin-fixed, paraffin-embedded human kidney tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.](http://images.novusbio.com/fullsize/Furin-Antibody-Immunohistochemistry-Paraffin-NBP2-24687-img0001.jpg "Immunohistochemistry-Paraffin: Furin Antibody [NBP2-24687] - analysis of Furin in formalin-fixed, paraffin-embedded human kidney tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.")
![Western Blot: Notch-2 Antibody [NB600-879] - Analysis of a band at 110 kDa corresponding to active Notch 2 protein (arrowhead). Analysis was performed for Notch 2 expression using 100ug of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constitutively active Notch 2 (intra cellular Notch 2). Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection. Lanes 4-6 are the corresponding control cells (untransfected) taken at similar time points. The band at about 110kD represents active Notch 2. This band is not seen in the control cell. The intracellular domain of Notch 2 has a predicted band size of 110kD, corresponding to this band. Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milk and probed with anti-Notch 2 at a 1:400 dilution. ECL was used as visualization method.](http://images.novusbio.com/fullsize/Notch-2-Antibody-Western-Blot-NB600-879-img0005.jpg "Western Blot: Notch-2 Antibody [NB600-879] - Analysis of a band at 110 kDa corresponding to active Notch 2 protein (arrowhead). Analysis was performed for Notch 2 expression using 100ug of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constitutively active Notch 2 (intra cellular Notch 2). Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection. Lanes 4-6 are the corresponding control cells (untransfected) taken at similar time points. The band at about 110kD represents active Notch 2. This band is not seen in the control cell. The intracellular domain of Notch 2 has a predicted band size of 110kD, corresponding to this band. Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milk and probed with anti-Notch 2 at a 1:400 dilution. ECL was used as visualization method.")
![Immunohistochemistry-Paraffin: Notch-2 Antibody [NB600-879] - Diluted 1:500 to detect Notch-2 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.](http://images.novusbio.com/fullsize/Notch-2-Antibody-Immunohistochemistry-Paraffin-NB600-879-img0003.jpg)
![Western Blot: Mouse Serum Albumin Antibody [NBP2-26510] - Western Blot of Mouse Serum Albumin antibody. Lane 1: molecular weight. Lane 2: mouse Mouse Serum Albumin. Load: 0.1 ug.Primary antibody: MSA antibody at 1:1000 in 1% BSA-TTBS for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:20,000 for 45 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting 30 min at 4C.Predicted/Observed size: 69 kDa, 72 kDa for MSA. Other band(s): none.](http://images.novusbio.com/fullsize/Mouse-Serum-Albumin-Antibody-Western-Blot-NBP2-26510-img0010.jpg "Western Blot: Mouse Serum Albumin Antibody [NBP2-26510] - Western Blot of Mouse Serum Albumin antibody. Lane 1: molecular weight. Lane 2: mouse Mouse Serum Albumin. Load: 0.1 ug.Primary antibody: MSA antibody at 1:1000 in 1% BSA-TTBS for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:20,000 for 45 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting 30 min at 4C.Predicted/Observed size: 69 kDa, 72 kDa for MSA. Other band(s): none.")
![Western Blot: Mouse Serum Albumin Antibody [NBP2-26510] - Western Blot: Albumin Antibody [Biotin] [NB600-929] - Lane 1: Human Serum Albumin. Load: 50 ng per lane. Primary antibody: Rabbit anti-Human Serum Albumin Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 66 kDa, 60 kDa for Human Serum Albumin. Image using the Biotin form of this antibody.](http://images.novusbio.com/fullsize/Mouse-Serum-Albumin-Antibody-Western-Blot-NBP2-26510-img0005.jpg)
![SDS-Page: Mouse Serum Albumin Antibody [NBP2-26510]](http://images.novusbio.com/fullsize/Mouse-Serum-Albumin-Antibody-SDS-Page-NBP2-26510-img0007.jpg)
![SDS-Page: Mouse Serum Albumin Antibody [NBP2-26510] - SDS-PAGE of Mouse Mouse Serum Albumin antibody.Lane 1: mouse serum albumin reduced. Lane 2: molecular weight. Lane 3: mouse serum albumin non-reduced. Load: 1.0 ug.Predicted/Observed size: Analysis by SDS-PAGE shows a banding pattern consistent with purified Rabbit IgG.Other band(s): none.](http://images.novusbio.com/fullsize/Mouse-Serum-Albumin-Antibody-SDS-Page-NBP2-26510-img0011.jpg)
![ELISA: Mouse Serum Albumin Antibody [NBP2-26510] - ELISA results of purified Mouse Mouse Serum Albumin antibody tested against Mouse Mouse Serum Albumin. Each well was coated in duplicate with 1.0 ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.](http://images.novusbio.com/fullsize/Mouse-Serum-Albumin-Antibody-ELISA-NBP2-26510-img0012.jpg)
![Western Blot: GOLGB1/Giantin Antibody (PSH03-86) [NBP3-32381] - Western blot analysis of GOLGB1/Giantin on different lysates with Rabbit anti-GOLGB1/Giantin antibody (NBP3-32381) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: Jurkat cell lysate (20 ug/Lane) Lane 4: HepG2 cell lysate (20 ug/Lane) Lane 5: Vero cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: PC-12 cell lysate (20 ug/Lane) Lane 8: Mouse lung tissue lysate (40 ug/Lane) Lane 9: Mouse small intestine tissue lysate (40 ug/Lane) Lane 10: Rat lung tissue lysate (40 ug/Lane) Predicted band size: 376 kDa Observed band size: 376 kDa Exposure time: Lane 1-6: 5 seconds; Lane 7-10: 1 minute 2 seconds; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32381) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32381_rabbit-golgb1-giantin-mab-psh03-86-278202416331281.jpg "Western Blot: GOLGB1/Giantin Antibody (PSH03-86) [NBP3-32381] - Western blot analysis of GOLGB1/Giantin on different lysates with Rabbit anti-GOLGB1/Giantin antibody (NBP3-32381) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: Jurkat cell lysate (20 ug/Lane) Lane 4: HepG2 cell lysate (20 ug/Lane) Lane 5: Vero cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: PC-12 cell lysate (20 ug/Lane) Lane 8: Mouse lung tissue lysate (40 ug/Lane) Lane 9: Mouse small intestine tissue lysate (40 ug/Lane) Lane 10: Rat lung tissue lysate (40 ug/Lane) Predicted band size: 376 kDa Observed band size: 376 kDa Exposure time: Lane 1-6: 5 seconds; Lane 7-10: 1 minute 2 seconds; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32381) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: GOLGB1/Giantin Antibody (PSH03-86) [NBP3-32381] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-GOLGB1/Giantin antibody (NBP3-32381) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32381) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32381_rabbit-golgb1-giantin-mab-psh03-86-298202414161858.jpg)
![Immunocytochemistry/ Immunofluorescence: GOLGB1/Giantin Antibody (PSH03-86) [NBP3-32381] - Immunocytochemistry analysis of HeLa cells labeling GOLGB1/Giantin with Rabbit anti-GOLGB1/Giantin antibody (NBP3-32381) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GOLGB1/Giantin antibody (NBP3-32381) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32381_rabbit-golgb1-giantin-mab-psh03-86-2920241114824.jpg)
![Flow Cytometry: GOLGB1/Giantin Antibody (PSH03-86) [NBP3-32381] - Flow cytometric analysis of HeLa cells labeling GOLGB1/Giantin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32381, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32381_rabbit-golgb1-giantin-mab-psh03-86-39202488463.jpg)
![Western Blot: Transferrin Antibody [NBP1-97472] - Transferrin blot (C) or Ponceau S staining (D) was used as loading control. **P < 0.01 (two-tailed unpaired t test), n = 3 mice per group; values represent the mean +/- SEM. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33087487) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Transferrin-Antibody-Western-Blot-NBP1-97472-img0002.jpg "Western Blot: Transferrin Antibody [NBP1-97472] - Transferrin blot (C) or Ponceau S staining (D) was used as loading control. **P < 0.01 (two-tailed unpaired t test), n = 3 mice per group; values represent the mean +/- SEM. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33087487) licensed under a CC-BY license.")
![Western Blot: Transferrin Antibody [NBP1-97472] - Analysis of rat apotransferrin protein probed with transferrin pAb.](http://images.novusbio.com/fullsize/Transferrin-Antibody-Western-Blot-NBP1-97472-img0001.jpg)
![Western Blot: Transferrin Antibody [NBP1-97472] - Transfer of hippocampal/hypothalamic PTMS to other brain regions & the blood.(A, B) 3-mo male C57BL/6 mice were injected with a dose of synapsin promoter-driven PTMS-HA lentivirus in one side of the posterior hypothalamus (A) or both sides of the hippocampus targeting CA1 & dentate gyrus (B). At 2 wk post injection, brain sections were immunostained for HA in the injected regions versus other brain areas. Small arrows pointed to some HA positive cells in transfer brain regions. Scale bar, 100 μm. Additional information of control lentivirus injections are presented in Fig S6. (C, D) 3-mo male C57BL/6 mice received bilateral injections of neuron-specific lentivirus of synapsin promoter-driven PTMS-HA (labelled as “PTMS-HA”) versus Control-HA (labelled as “Con-HA”) in the hippocampus (targeting CA1 & dentate gyrus) & posterior hypothalamus, & at 2-wk post injection, secretion of PTMS-HA to the CSF (C) & the serum (D) was assessed via IP-Western blotting. (C, D) Transferrin blot (C) or Ponceau S staining (D) was used as loading control. **P < 0.01 (two-tailed unpaired t test), n = 3 mice per group; values represent the mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33087487), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97472_rabbit-polyclonal-transferrin-antibody-31020241612216.jpg)
![Western Blot: Transferrin Antibody [NBP1-97472] - PTMS secretion declines during aging.(A, B) PTMS levels in the CSF of male C57BL/6 mice at young (∼4 mo) or old (∼24 mo) age were measured by Western blot (A) which were quantified in panel (B). Transferrin was used to as a loading control. (C) Young (3-mo) or middle aged (18-mo) male C57BL/6 mice received unilateral injection of the same dose of Sox2 promoter-driven PTMS-HA lentivirus in the mediobasal hypothalamus. White arrows point to the site of injections. At 1 wk postinjection, hypothalamic sections were generated for HA immunostaining to show the levels of both primary expression of PTMS-HA in Sox2-positive cells & secondary transfer of PTMS-HA into Sox2-negative cells. Sox2 co-immunostaining was performed to reveal Sox2-positive cells in this region. Lower panels in show high-magnification views of outlined areas. Scale bar, 100 μm. 3V: hypothalamic third ventricle. *P < 0.05, two-tailed unpaired t test (B), n = 3 mice per group; values represent the mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33087487), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97472_rabbit-polyclonal-transferrin-antibody-3102024161227.jpg)
![Western Blot: Transferrin Antibody [NBP1-97472] - Contribution of the hypothalamus to PTMS in the CSF.(A) PTMS levels in blood serum versus CSF were detected by immunoprecipitation (IP)-Western blot (left panel), & the band intensities (au, arbitrary unit) were quantified (right panel). Matched IgG was used as a negative control of IP procedure, & Commassie staining was used as a loading control. (B, C) Injection of ptms-shRNA (sh-ptms) or scramble control shRNA (sh-control) lentiviruses into hypothalamic third ventricle of 3-mo male C57BL/6 mice (B), & at 1 mo postinjection, PTMS levels in CSF were measured by Western blot (left panel, transferrin as a loading control) & quantified (right panel). Scale bar, 100 μm. (A, C) *P < 0.05 (two-tailed unpaired t test), n = 4 mice (A) or 3 mice (C) per group; values represent the mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33087487), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97472_rabbit-polyclonal-transferrin-antibody-31020241612226.jpg)
![Western Blot: ATG9B Antibody [NBP1-77169] - HeLa cell lysate with ATG9B antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/ATG9B-Antibody-Western-Blot-NBP1-77169-img0001.jpg "Western Blot: ATG9B Antibody [NBP1-77169] - HeLa cell lysate with ATG9B antibody at (A) 1 and (B) 2 ug/mL.")
![Immunocytochemistry/ Immunofluorescence: ATG9B Antibody - BSA Free [NBP1-77169] - Immunofluorescence of ATG9B in Hela cells with ATG9B antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77169_rabbit-polyclonal-atg9b-antibody-2711202419313264.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG9B Antibody - BSA Free [NBP1-77169] - Immunocytochemistry of ATG9B in HeLa cells with ATG9B antibody at 10 u/mL.](http://images.novusbio.com/fullsize/nbp1-77169_rabbit-polyclonal-atg9b-antibody-2711202420212356.jpg)
![Western Blot: COPG2 Antibody [NBP2-92868] - Analysis of extracts of various cell lines, using COPG2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/COPG2-Antibody-Western-Blot-NBP2-92868-img0005.jpg "Western Blot: COPG2 Antibody [NBP2-92868] - Analysis of extracts of various cell lines, using COPG2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: COPG2 Antibody [NBP2-92868] - Analysis of U-2 OS cells using COPG2 . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/COPG2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92868-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: COPG2 Antibody - Azide and BSA Free [NBP2-92868] - Immunofluorescence analysis of C6 cells using COPG2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92868_rabbit-polyclonal-copg2-antibody-42202514264485.jpg)
![Immunohistochemistry-Paraffin: COPG2 Antibody [NBP2-92868] - Rat brain using COPG2 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/COPG2-Antibody-Immunohistochemistry-Paraffin-NBP2-92868-img0004.jpg)
![Immunohistochemistry-Paraffin: COPG2 Antibody [NBP2-92868] - Mouse brain using COPG2 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/COPG2-Antibody-Immunohistochemistry-Paraffin-NBP2-92868-img0003.jpg)