| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-40823SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-330SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44493 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08957 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87038-25ul | |||
![Western Blot: MSH2 Antibody [NB100-621] - Detection of Human and Mouse MSH2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MSH2 antibody NB100-621 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MSH2-Antibody-Western-Blot-NB100-621-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: MSH2 Antibody [NB100-621] - Detection of Human and Mouse MSH2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MSH2 antibody NB100-621 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MSH2 Antibody [NB100-621] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MSH2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MSH2-Antibody-Immunohistochemistry-Paraffin-NB100-621-img0003.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: MSH2 Antibody [NB100-621] - Detection of human and mouse MSH2 by Western blot and immunoprecipitation using NB100-621. Detection: ECL with a 3 minute exposure.](http://images.novusbio.com/fullsize/MSH2-Antibody-Western-Blot-NB100-621-img0001.jpg)
![Immunohistochemistry-Paraffin: MSH2 Antibody [NB100-621] - FFPE section of mouse renal cell carcinoma. Antibody:Affinity purified rabbit anti-MSH2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MSH2-Antibody-Immunohistochemistry-Paraffin-NB100-621-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: MSH2 Antibody [NB100-621] - Detection of human MSH2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MSH2 antibody NB100-621 used for IP at 6 ug per reaction. MSH2 was also immunoprecipitated by another rabbit anti-MSH2 antibody. For blotting immunoprecipitated MSH2, NB100-621 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MSH2-Antibody-Immunoprecipitation-NB100-621-img0009.jpg)
![Western Blot: Bmi1 Antibody [NB110-40823] - Detection of Bmi1 protein in skeletal muscle](http://images.novusbio.com/fullsize/Bmi1-Antibody-Western-Blot-NB110-40823-img0002.jpg "Western Blot: Bmi1 Antibody [NB110-40823] - Detection of Bmi1 protein in skeletal muscle")
![Immunocytochemistry/Immunofluorescence: Bmi1 Antibody [NB110-40823] - BMI1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Bmi1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40823-img0004.jpg)
![Western Blot: BTF Antibody [NB100-1704] - Whole cell lysate from HeLa (5, 15, 50 ug loaded) and 293T cells (T; 50 ug) probed with anti-BTF antibody at 0.04 ug/ml.](http://images.novusbio.com/fullsize/BTF-Antibody-Western-Blot-NB100-1704-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: BTF Antibody [NB100-1704] - Whole cell lysate from HeLa (5, 15, 50 ug loaded) and 293T cells (T; 50 ug) probed with anti-BTF antibody at 0.04 ug/ml.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: BTF Antibody [NB100-1704] - Sample: FFPE section of human skin carcinoma. Antibody: Affinity purified rabbit anti-BTF used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.](http://images.novusbio.com/fullsize/BTF-Antibody-Immunohistochemistry-NB100-1704-img0006.jpg)
![Western Blot: BTF Antibody [NB100-1704] - : Whole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells.](http://images.novusbio.com/fullsize/BTF-Antibody-Western-Blot-NB100-1704-img0003.jpg)
![Immunoprecipitation: BTF Antibody [NB100-1704] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells.](http://images.novusbio.com/fullsize/BTF-Antibody-Immunoprecipitation-NB100-1704-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Separase Antibody (XJ11-4D7) [NB100-330] - Overlay [blue] of centrosomal staining in HeLa cells. Centrosomal staining of separase [yellow], using NB 100-330, and gamma tubulin [green] in mitotic-metaphase cells and nuclear staining of separase in pre-mitotic cells.](http://images.novusbio.com/fullsize/Separase-Antibody-XJ11-4D7-Immunocytochemistry-Immunofluorescence-NB100-330-img0003.jpg "Immunocytochemistry/Immunofluorescence: Separase Antibody (XJ11-4D7) [NB100-330] - Overlay [blue] of centrosomal staining in HeLa cells. Centrosomal staining of separase [yellow], using NB 100-330, and gamma tubulin [green] in mitotic-metaphase cells and nuclear staining of separase in pre-mitotic cells.")
![Western Blot: HDAC1 Antibody [NBP1-78101] - Western Blot of HDAC1 antibody. Lane 1: LNCaP prostate cancer cells. Load: 50 ug per lane.Primary antibody: HDAC1 antibody at 1:1000 for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.Predicted/Observed size: 55kDa for HDAC1.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-78101-img0012.jpg "Western Blot: HDAC1 Antibody [NBP1-78101] - Western Blot of HDAC1 antibody. Lane 1: LNCaP prostate cancer cells. Load: 50 ug per lane.Primary antibody: HDAC1 antibody at 1:1000 for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.Predicted/Observed size: 55kDa for HDAC1.")
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP1-78101] - Immunofluorescence Microscopy of HDAC1 antibody. Tissue: A431 cells. Fixation: methanol. Antigen retrieval: blocked with normal goat serum.Primary antibody: HDAC1 antibody at 4 ug/mL for 1 h at RT.Secondary antibody: rabbit secondary antibody at 0.2 ug/mL for 45 min at RT.Localization: HDAC1 is nuclear.Staining: HDAC1 as green fluorescent signal. A-tubulin monoclonal antibody detected with ATTO 425 (colored RED). 2-color STED image, Leica Microsystems.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78101-img0010.jpg)
![Immunohistochemistry: HDAC1 Antibody [NBP1-78101] - Immunohistochemistry of HDAC1 antibody. Tissue: human lung tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: HDAC1 antibody at 10 ug/mL for 1 h at RT.Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.Localization: HDAC1 is nuclear.Staining: HDAC1 as brown color indicates presence of protein, blue color shows cell nuclei.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-NBP1-78101-img0014.jpg)
![Western Blot: HDAC1 Antibody [NBP1-78101] - Western Blot of HDAC1 antibody. Lane 1: 293 whole cell lysate. Load: 35 ug per lane.Primary antibody: HDAC1 antibody at 1:3,500 for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.Predicted/Observed size: ~65 kDa corresponding to human HDAC1. Other band(s): none.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-78101-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP1-78101] - Rabbit IgG 611-151-122 (shown in red) for to detect HDAC-1 by immunofluorescence. Anti Keratin monoclonal antibody was used with Dylight 488 goat anti mouse (shown in green) to detect Keratin](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78101-img0004.jpg)
![Immunohistochemistry: HDAC1 Antibody [NBP1-78101] - Immunohistochemistry of HDAC1 antibody. Tissue: human prostate cancer tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: HDAC1 antibody at 1:500 for 1 h at RT.Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.Localization: HDAC1 is nuclear.Staining: HDAC1 precipitated purple with blue counterstain.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-NBP1-78101-img0013.jpg)
![Western Blot: eIF2A [p Ser51] Antibody [NB100-81896] - Analysis of extracts from Jurkat cells untreated or treated with H2O2 using eIF2 alpha (Phospho-Ser51) antibody.](http://images.novusbio.com/fullsize/eIF2A-[p-Ser51]-Antibody-Western-Blot-NB100-81896-img0009.jpg "<b>Biological Strategies Validation. </b>Western Blot: eIF2A [p Ser51] Antibody [NB100-81896] - Analysis of extracts from Jurkat cells untreated or treated with H2O2 using eIF2 alpha (Phospho-Ser51) antibody.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody [NB100-81896] - Paraffin-embedded human lung carcinoma tissue.](http://images.novusbio.com/fullsize/eIF2A-[p-Ser51]-Antibody-Immunohistochemistry-Paraffin-NB100-81896-img0011.jpg)
![Western Blot: eIF2A [p Ser51] Antibody [NB100-81896] - Western blot analysis of lysates from 1) KB, 2) MCF-7 cells, (Green) primary antibody was diluted at 1: 1000,](http://images.novusbio.com/fullsize/nb100-81896_rabbit-polyclonal-eif2a-p-ser51-antibody-23420241523258.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody [NB100-81896] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NB100-81896(left) or the same antibody preincubated with blocking peptide (right).](http://images.novusbio.com/fullsize/nb100-81896_rabbit-polyclonal-eif2a-p-ser51-antibody-103202523464519.jpg)
![Immunocytochemistry/Immunofluorescence: eIF2A [p Ser51] Antibody [NB100-81896] - Staining of methanol-fixed HeLa cells using NB100-81896](http://images.novusbio.com/fullsize/nb100-81896_rabbit-polyclonal-eif2a-p-ser51-antibody-1132025155593.jpg)
![Immunocytochemistry/Immunofluorescence: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718] - Analysis of PFA-fixed MCF-7 cells labeling p27 with p27/Kip1 Antibody (SX53G8) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-SX53G8-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-34718-img0001.jpg "Immunocytochemistry/Immunofluorescence: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718] - Analysis of PFA-fixed MCF-7 cells labeling p27 with p27/Kip1 Antibody (SX53G8) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.")
![Immunohistochemistry-Paraffin: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718] - p27/Kip1 antibody in CODEX stain of human bone marrow tissue. Image from verified customer review.](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-SX53G8-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34718-img0007.jpg)
![Flow Cytometry: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718] - Flow Cytometry of human p27/Kip1 on HeLa cells. Black: cells alone; Grey: Isotype Control; Green: CF488-labeled p27/Kip1 Monoclonal Antibody (SX53G8).](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-SX53G8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-34718-img0006.jpg)
![Immunohistochemistry-Paraffin: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718] - Formalin-paraffin human colon stained with p27 MAb (SX53G8)](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-SX53G8-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34718-img0004.jpg)
![Immunohistochemistry-Paraffin: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718] - Formalin-fixed, paraffin-embedded human prostate Carcinoma stained with p27 Monoclonal Antibody (sx53G8)](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-SX53G8-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34718-img0005.jpg)
![Flow Cytometry: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718] - Analysis of PFA-fixed MCF-7 cells using p27/Kip1 Antibody (SX53G8) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-SX53G8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-34718-img0003.jpg)
![Western Blot: MSH2 Antibody (MSH2/2622) [NBP3-07211] - Western Blot Analysis of human HepG2, A549, and A431 cell lysate using MSH2 Mouse Monoclonal Antibody (MSH2/2622).](http://images.novusbio.com/fullsize/MSH2-Antibody-MSH2-2622-Western-Blot-NBP3-07211-img0007.jpg "Western Blot: MSH2 Antibody (MSH2/2622) [NBP3-07211] - Western Blot Analysis of human HepG2, A549, and A431 cell lysate using MSH2 Mouse Monoclonal Antibody (MSH2/2622).")
![Immunocytochemistry/Immunofluorescence: MSH2 Antibody (MSH2/2622) [NBP3-07211] - Immunofluorescence staining of MOLT-4 cells using MSH2 Mouse Monoclonal Antibody (MSH2/2622) followed by goat anti- Mouse IgG-CF488 (green). Membrane are stained with Phalloidin (Red).](http://images.novusbio.com/fullsize/MSH2-Antibody-MSH2-2622-Immunocytochemistry-Immunofluorescence-NBP3-07211-img0002.jpg)
![Immunohistochemistry-Paraffin: MSH2 Antibody (MSH2/2622) [NBP3-07211] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MSH2 Mouse Monoclonal Antibody (MSH2/2622).](http://images.novusbio.com/fullsize/MSH2-Antibody-MSH2-2622-Immunohistochemistry-Paraffin-NBP3-07211-img0006.jpg)
![Flow Cytometry: MSH2 Antibody (MSH2/2622) [NBP3-07211] - Flow Cytometric Analysis of A549 cells using MSH2 Mouse Monoclonal Antibody (MSH2/2622) followed by goat anti- Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/MSH2-Antibody-MSH2-2622-Flow-Cytometry-NBP3-07211-img0001.jpg)
![Immunohistochemistry-Paraffin: MSH2 Antibody (MSH2/2622) [NBP3-07211] - Formalin-fixed, paraffin-embedded human Colon from Lynch disease stained with MSH2 Mouse Monoclonal Antibody (MSH2/2622).](http://images.novusbio.com/fullsize/MSH2-Antibody-MSH2-2622-Immunohistochemistry-Paraffin-NBP3-07211-img0003.jpg)
![Immunohistochemistry-Paraffin: MSH2 Antibody (MSH2/2622) [NBP3-07211] - Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with MSH2 Mouse Monoclonal Antibody (MSH2/2622).](http://images.novusbio.com/fullsize/MSH2-Antibody-MSH2-2622-Immunohistochemistry-Paraffin-NBP3-07211-img0004.jpg)
![Immunohistochemistry-Paraffin: MSH2 Antibody (MSH2/2622) [NBP3-07211] - Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with MSH2 Mouse Monoclonal Antibody (MSH2/2622).](http://images.novusbio.com/fullsize/MSH2-Antibody-MSH2-2622-Immunohistochemistry-Paraffin-NBP3-07211-img0005.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody [NBP1-87038] - Staining of human lymph node shows moderate to strong nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87038-img0009.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody [NBP1-87038] - Saining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87038-img0006.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody [NBP1-87038] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87038-img0007.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody [NBP1-87038] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87038-img0008.jpg)
![Western Blot: 53BP1 Antibody (HL275) [NBP3-13664] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (NBP3-13664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/53BP1-Antibody-HL275-Western-Blot-NBP3-13664-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: 53BP1 Antibody (HL275) [NBP3-13664] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (NBP3-13664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: 53BP1 Antibody (HL275) [NBP3-13664] - 53BP1 antibody [HL275] detects 53BP1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: 53BP1 stained by 53BP1 antibody [HL275] (NBP3-13664) diluted at 1:1000. Blue: Fluoroshield with DAPI. Scale bar= 10 um.](http://images.novusbio.com/fullsize/53BP1-Antibody-HL275-Immunocytochemistry-Immunofluorescence-NBP3-13664-img0003.jpg)
![Immunohistochemistry-Paraffin: 53BP1 Antibody (HL275) [NBP3-13664] - 53BP1 antibody [HL275] detects 53BP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. 53BP1 stained by 53BP1 antibody [HL275] (NBP3-13664) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/53BP1-Antibody-HL275-Immunohistochemistry-Paraffin-NBP3-13664-img0002.jpg)
![Western Blot: 53BP1 Antibody (HL275) [NBP3-13664] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (NBP3-13664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/53BP1-Antibody-HL275-Western-Blot-NBP3-13664-img0004.jpg)
![Western Blot: 53BP1 Antibody (HL275) [NBP3-13664] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (NBP3-13664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/53BP1-Antibody-HL275-Western-Blot-NBP3-13664-img0005.jpg)
![Western Blot: 53BP1 Antibody (HL275) [NBP3-13664] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (NBP3-13664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/53BP1-Antibody-HL275-Western-Blot-NBP3-13664-img0006.jpg)
![Immunohistochemistry-Paraffin: 53BP1 Antibody (HL275) [NBP3-13664] - 53BP1 antibody [HL275] detects 53BP1 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.53BP1 stained by 53BP1 antibody [HL275] (NBP3-13664) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13664_rabbit-53bp1-mab-hl275-91020242011411.jpg)
![Immunohistochemistry-Paraffin: 53BP1 Antibody (HL275) [NBP3-13664] - 53BP1 antibody [HL275] detects 53BP1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.53BP1 stained by 53BP1 antibody [HL275] (NBP3-13664) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13664_rabbit-53bp1-mab-hl275-910202420115086.jpg)
![Western Blot: 53BP1 Antibody (HL275) [NBP3-13664] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (NBP3-13664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13664_rabbit-53bp1-mab-hl275-91020242021972.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: 53BP1 Antibody (HL275) [NBP3-13664] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL275] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-13664_rabbit-53bp1-mab-hl275-1610202419405743.jpg)
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL275] (NBP3-13664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-13664_rabbit-53bp1-mab-hl275-western-blot-2611202518303816.jpg)
![Western Blot: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-2120249374982.jpg "Western Blot: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-2120249383186.jpg)
![Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Thymidylate synthase antibody [HL1236] detects Thymidylate synthase protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded dog tissue. Thymidylate synthase stained by Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-2120249355536.jpg)
![Western Blot: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-2120249403876.jpg)
![Immunocytochemistry/Immunofluorescence: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Thymidylate synthase antibody [HL1236] detects Thymidylate synthase protein at cytoplasm and nucleus and mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Thymidylate synthase stained by Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-212024937157.jpg)
![Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Thymidylate synthase antibody [HL1236] detects Thymidylate synthase protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded cat brain. Thymidylate synthase stained by Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-2120249415929.jpg)
![Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Thymidylate synthase antibody [HL1236] detects Thymidylate synthase protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded dog brain. Thymidylate synthase stained by Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-212024941337.jpg)
![Western Blot: Histone H2AX Antibody (BLR053F) [NBP3-14678] - Whole cell lysate (50 ug) from Jurkat cells treated with 100 uM etoposide (+) or mock treated (-). Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody [BLR053F] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/gamma-H2AX-Antibody-BLR053F-Western-Blot-NBP3-14678-img0002.jpg "Western Blot: Histone H2AX Antibody (BLR053F) [NBP3-14678] - Whole cell lysate (50 ug) from Jurkat cells treated with 100 uM etoposide (+) or mock treated (-). Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody [BLR053F] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 seconds.")
![Immunocytochemistry/Immunofluorescence: Histone H2AX Antibody (BLR053F) [NBP3-14678] - Section of human HeLa cells treated with etoposide. Antibody: Rabbit Histone H2AX antibody (BLR053F) used at 1:250. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate:DAB.](http://images.novusbio.com/fullsize/gamma-H2AX-Antibody-BLR053F-Immunocytochemistry-Immunofluorescence-NBP3-14678-img0006.jpg)
![Immunohistochemistry-Paraffin: Histone H2AX Antibody (BLR053F) [NBP3-14678] - Section of human breast carcinoma. Antibody: Rabbit Histone H2AX antibody (BLR053F) used at 1:100. Secondary: DyLight(R) 594-conjugated goat anti-rabbit IgG. Counterstain: DAPI.](http://images.novusbio.com/fullsize/gamma-H2AX-Antibody-BLR053F-Immunohistochemistry-Paraffin-NBP3-14678-img0007.jpg)
![Flow Cytometry: Histone H2AX Antibody (BLR053F) [NBP3-14678] - Rabbit Histone H2AX antibody (BLR053F) or isotype control (unshaded). Secondary: DyLight(R) 488-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/gamma-H2AX-Antibody-BLR053F-Flow-Cytometry-NBP3-14678-img0008.jpg)
![Immunohistochemistry-Paraffin: Histone H2AX Antibody (BLR053F) [NBP3-14678] - Section of human basal cell carcinoma. Antibody: Rabbit Histone H2AX antibody (BLR053F) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate:DAB.](http://images.novusbio.com/fullsize/gamma-H2AX-Antibody-BLR053F-Immunohistochemistry-Paraffin-NBP3-14678-img0003.jpg)
![Immunohistochemistry-Paraffin: Histone H2AX Antibody (BLR053F) [NBP3-14678] - Section of mouse CT26 tumor. Antibody: Rabbit Histone H2AX antibody (BLR053F) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate:DAB.](http://images.novusbio.com/fullsize/gamma-H2AX-Antibody-BLR053F-Immunohistochemistry-Paraffin-NBP3-14678-img0005.jpg)
![Immunoprecipitation: Histone H2AX Antibody (BLR053F) [NBP3-14678] - Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells treated with 100 uM etoposide (+) or mock treated (-). Antibodies: Rabbit anti-gamma-H2AX recombinant monoclonal antibody used for IP at 20 ul per reaction. gamma-H2AX was also immunoprecipitated by rabbit anti-gamma-H2AX antibody. For blotting immunoprecipitated gamma-H2AX, was used at 1:1000. Chemiluminescence with an exposure time of 3 seconds. Lower panel: Rabbit anti-H2AX](http://images.novusbio.com/fullsize/gamma-H2AX-Antibody-Immunoprecipitation-NBP3-14678-img0001.jpg)