| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61930SS | |||
| Azide and BSA Free | NBP2-80730 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33995-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-14361-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86561-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98982-50ul | |||
![Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021] - Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates transfected with various plk-1 mutation constructs. Blots were reacted with anti-Plk-1 pT210 (panel A) and pan reactive anti-Plk-1 (panel B). Transfected cells were treated with 1 uM nocodazole followed by cell collection, lysate preparation, SDS-PAGE and western blotting. Using a 1:1000 dilution, anti-Plk-1 pT210 detects a single band corresponding to endogenous plk-1, but does not detect recombinant forms of the protein presumably because of a lack of phosphorylation in these mutants.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Western-Blot-NBP1-78021-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021] - Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates transfected with various plk-1 mutation constructs. Blots were reacted with anti-Plk-1 pT210 (panel A) and pan reactive anti-Plk-1 (panel B). Transfected cells were treated with 1 uM nocodazole followed by cell collection, lysate preparation, SDS-PAGE and western blotting. Using a 1:1000 dilution, anti-Plk-1 pT210 detects a single band corresponding to endogenous plk-1, but does not detect recombinant forms of the protein presumably because of a lack of phosphorylation in these mutants.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: PLK1 [p Thr210] Antibody [NBP1-78021] - Used at a 1:200 dilution to detect Plk1 by immunohistochemistry in human breast carcinoma tumor tissue. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Immunohistochemistry-NBP1-78021-img0006.jpg)
![Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021] - Plk-1 pT210 antibody to detect endogenous protein present in a Mouse A20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~68 kDa corresponding to Plk-1 protein. It is suggested to use a nuclear extract from synchronized cells to greatly increase the abundance of this protein in preparations. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Western-Blot-NBP1-78021-img0002.jpg)
![Immunohistochemistry: PLK1 [p Thr210] Antibody [NBP1-78021] - Used at a 1:200 dilution to detect Plk1 by immunohistochemistry in human colon carcinoma tumor tissue. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Immunohistochemistry-NBP1-78021-img0007.jpg)
![Western Blot: FANCD2 Antibody (1290C) [NBP2-61930] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FANCD2 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/mL Rabbit Monoclonal FANCD2 Antibody (1290C) (Catalog # NBP2-61930) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FANCD2 at approximately 150 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/FANCD2-Antibody-1290C-Knockout-Validated-NBP2-61930-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: FANCD2 Antibody (1290C) [NBP2-61930] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FANCD2 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/mL Rabbit Monoclonal FANCD2 Antibody (1290C) (Catalog # NBP2-61930) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FANCD2 at approximately 150 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: FANCD2 Antibody (1290C) [NBP2-61930] - Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line (Molecular weight: 164.1 KDa), Hepa 1-6 mouse hepatoma cell line, and H4-II-E-C3 rat hepatoma cell line. PVDF membrane was probed with 0.5 ug/mL of Rabbit Monoclonal FANCD2 Antibody (1290C) (Catalog # NBP2-61930) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for FANCD2 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/FANCD2-Antibody-1290C-Western-Blot-NBP2-61930-img0004.jpg)
![Immunohistochemistry-Paraffin: ATR Antibody (1E9) [H00000545-M03] - Analysis of monoclonal antibody to ATR on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/ATR-Antibody-1E9-Immunohistochemistry-Paraffin-H00000545-M03-img0002.jpg "Immunohistochemistry-Paraffin: ATR Antibody (1E9) [H00000545-M03] - Analysis of monoclonal antibody to ATR on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml")
![Western Blot: Rad51C Antibody (3F3-5C6) [H00005889-M01] - Analysis of RAD51C expression in transfected 293T cell line by RAD51C monoclonal antibody (M01), clone 3F3-5C6.Lane 1: RAD51C transfected lysate(42.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Rad51C-Antibody-3F3-5C6-Western-Blot-H00005889-M01-img0013.jpg "Western Blot: Rad51C Antibody (3F3-5C6) [H00005889-M01] - Analysis of RAD51C expression in transfected 293T cell line by RAD51C monoclonal antibody (M01), clone 3F3-5C6.Lane 1: RAD51C transfected lysate(42.2 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Rad51C Antibody (3F3-5C6) [H00005889-M01] - Analysis of monoclonal antibody to RAD51C on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Rad51C-Antibody-3F3-5C6-Immunocytochemistry-Immunofluorescence-H00005889-M01-img0008.jpg)
![Western Blot: Rad51C Antibody (3F3-5C6) [H00005889-M01] - Analysis of RAD51C over-expressed 293 cell line, cotransfected with RAD51C Validated Chimera RNAi ( Cat # H00005889-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RAD51C monoclonal antibody (M01), clone 3F3-5C6 (Cat # H00005889-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/Rad51C-Antibody-3F3-5C6-Western-Blot-H00005889-M01-img0009.jpg)
![Western Blot: Rad51C Antibody (3F3-5C6) [H00005889-M01] - RAD51C monoclonal antibody (M01), clone 3F3-5C6 Analysis of RAD51C expression in HeLa.](http://images.novusbio.com/fullsize/Rad51C-Antibody-3F3-5C6-Western-Blot-H00005889-M01-img0011.jpg)
![Western Blot: Rad51C Antibody (3F3-5C6) [H00005889-M01] - RAD51C monoclonal antibody (M01), clone 3F3-5C6. Analysis of RAD51C expression in 293.](http://images.novusbio.com/fullsize/Rad51C-Antibody-3F3-5C6-Western-Blot-H00005889-M01-img0012.jpg)
![ELISA: Rad51C Antibody (3F3-5C6) [H00005889-M01] - Detection limit for recombinant GST tagged RAD51C is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Rad51C-Antibody-3F3-5C6-ELISA-H00005889-M01-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: DNA-PKcs Antibody [NBP2-33995] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33995-img0006.jpg "Immunocytochemistry/Immunofluorescence: DNA-PKcs Antibody [NBP2-33995] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: DNA-PKcs Antibody [NBP2-33995] - Staining of human endometrium shows strong nuclear positivity in glandular and stromal cells.](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-Immunohistochemistry-Paraffin-NBP2-33995-img0009.jpg)
![Immunohistochemistry-Paraffin: DNA-PKcs Antibody [NBP2-33995] - Staining of human lymph node shows strong nuclear positivity in lymphoid cells.](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-Immunohistochemistry-Paraffin-NBP2-33995-img0007.jpg)
![Immunohistochemistry-Paraffin: DNA-PKcs Antibody [NBP2-33995] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-Immunohistochemistry-Paraffin-NBP2-33995-img0008.jpg)
![Immunohistochemistry-Paraffin: DNA-PKcs Antibody [NBP2-33995] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-Immunohistochemistry-Paraffin-NBP2-33995-img0010.jpg)
![Western Blot: BRIP1/FANCJ Antibody [NBP2-14361] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-BRIP1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/BRIP1-FANCJ-Antibody-Western-Blot-NBP2-14361-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: BRIP1/FANCJ Antibody [NBP2-14361] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-BRIP1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: BRIP1/FANCJ Antibody [NBP2-14361] - Staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize/BRIP1-FANCJ-Antibody-Immunohistochemistry-Paraffin-NBP2-14361-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Rad17 Antibody [NBP1-86561] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/Rad17-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86561-img0006.jpg "Immunocytochemistry/Immunofluorescence: Rad17 Antibody [NBP1-86561] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: Rad17 Antibody [NBP1-86561] - Staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize/Rad17-Antibody-Immunohistochemistry-Paraffin-NBP1-86561-img0001.jpg)
![Western Blot: Ku80/XRCC5 Antibody (7N7G3) [NBP3-15448] - Western blot analysis of extracts of various cell lines, using Ku80/XRCC5 Rabbit mAb (NBP3-15448) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-7N7G3-Western-Blot-NBP3-15448-img0003.jpg "Western Blot: Ku80/XRCC5 Antibody (7N7G3) [NBP3-15448] - Western blot analysis of extracts of various cell lines, using Ku80/XRCC5 Rabbit mAb (NBP3-15448) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunohistochemistry-Paraffin: Ku80/XRCC5 Antibody (7N7G3) [NBP3-15448] - Immunohistochemistry of paraffin-embedded human esophageal using Ku80/XRCC5 Rabbit mAb (NBP3-15448) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-7N7G3-Immunohistochemistry-Paraffin-NBP3-15448-img0001.jpg)
![Immunoprecipitation: Ku80/XRCC5 Antibody (7N7G3) [NBP3-15448] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Ku80/XRCC5 antibody (NBP3-15448). Western blot was performed from the immunoprecipitate using Ku80/XRCC5 antibody (NBP3-15448) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-7N7G3-Immunoprecipitation-NBP3-15448-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Ku80/XRCC5 Antibody (7N7G3) [Ku80/XRCC5] - Confocal imaging of HeLa cells using Ku80/XRCC5 Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 60x.](http://images.novusbio.com/fullsize/nbp3-15448_rabbit-ku80-xrcc5-mab-7n7g3-52202517152746.jpg)
![Immunohistochemistry-Paraffin: ATR Antibody [NBP2-98982] - Immunochemical staining ATR in mouse testis with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ATR-Antibody-Immunohistochemistry-Paraffin-NBP2-98982-img0004.jpg "Immunohistochemistry-Paraffin: ATR Antibody [NBP2-98982] - Immunochemical staining ATR in mouse testis with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: ATR Antibody [NBP2-98982] - Immunochemical staining ATR in human prostate with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ATR-Antibody-Immunohistochemistry-Paraffin-NBP2-98982-img0002.jpg)
![Immunohistochemistry-Paraffin: ATR Antibody [NBP2-98982] - Immunochemical staining ATR in human testis with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ATR-Antibody-Immunohistochemistry-Paraffin-NBP2-98982-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: ATR Antibody [NBP2-98982] - Immunofluorescence staining of ATR in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98982 antibody (dilution ratio 1:500) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus .](http://images.novusbio.com/fullsize/nbp2-98982_rabbit-polyclonal-atr-antibody-23520251149408.png)
![Western Blot: Nbs1 Antibody [NB100-60654] - Detection of human Nbs1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Nbs1 was also immunoprecipitated by rabbit anti-Nbs1 antibodies NB100-60653 and NB100-60654, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/Nbs1-Antibody-Western-Blot-NB100-60654-img0017.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Nbs1 Antibody [NB100-60654] - Detection of human Nbs1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Nbs1 was also immunoprecipitated by rabbit anti-Nbs1 antibodies NB100-60653 and NB100-60654, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: Nbs1 Antibody [NB100-60654] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used at a dilution of 1:200 (1ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/Nbs1-Antibody-Immunohistochemistry-NB100-60654-img0027.jpg)
![Western Blot: PUMA Antibody [NB100-56623] - Analysis of PUMA in A) HeLa and B) NIH 3T3 cell lysate using this antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/PUMA-Antibody-Western-Blot-NB100-56623-img0002.jpg "Western Blot: PUMA Antibody [NB100-56623] - Analysis of PUMA in A) HeLa and B) NIH 3T3 cell lysate using this antibody at 5 ug/ml.")
![Western Blot: Rad17 [p Ser645] Antibody [NB100-273] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells treated with 100 uM EPE for 2 hours (+) or mock treated (-) cells. Antibodies : Affinity purified rabbit anti-Rad17, Phospho (S645) antibody . Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower panels).](http://images.novusbio.com/fullsize/Rad17-[p-Ser645]-Antibody-Western-Blot-NB100-273-img0045.jpg "<b>Biological Strategies Validation. </b>Western Blot: Rad17 [p Ser645] Antibody [NB100-273] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells treated with 100 uM EPE for 2 hours (+) or mock treated (-) cells. Antibodies : Affinity purified rabbit anti-Rad17, Phospho (S645) antibody . Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower panels).")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: Rad17 [p Ser645] Antibody [NB100-273] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-Phospho Rad17 (S645) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Rad17-[p-Ser645]-Antibody-Immunohistochemistry-NB100-273-img0049.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Rad17 [p Ser645] Antibody [NB100-273] - Whole cell lysate (50 ug) from HeLa cells before (-) or after (+) treatment with 10 Gy ionizing radiation. Antibody used at 1 ug/ml.](http://images.novusbio.com/fullsize/Rad17-[p-Ser645]-Antibody-Western-Blot-NB100-273-img0002.jpg)
![Immunohistochemistry: Rad17 [p Ser645] Antibody [NB100-273] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Phospho Rad17 (S645) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Rad17-[p-Ser645]-Antibody-Immunohistochemistry-NB100-273-img0048.jpg)