| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22196-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-19255-0.2mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52590-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56627SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61930SS | |||
| Azide and BSA Free | NBP2-80730 | |||
![Western Blot: PLK1 Antibody (3C11) [NBP2-22196] - Western blot analysis using PLK1 mouse mAb against K562 (1) and Raji (2) cell lysate.](http://images.novusbio.com/fullsize/PLK1-Antibody-3C11-Western-Blot-NBP2-22196-img0006.jpg "Western Blot: PLK1 Antibody (3C11) [NBP2-22196] - Western blot analysis using PLK1 mouse mAb against K562 (1) and Raji (2) cell lysate.")
![Immunohistochemistry-Paraffin: PLK1 Antibody (3C11) [NBP2-22196] - Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using PLK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PLK1-Antibody-3C11-Immunohistochemistry-Paraffin-NBP2-22196-img0003.jpg)
![Flow Cytometry: PLK1 Antibody (3C11) [NBP2-22196] - Flow cytometric analysis of NIH3T3 cells using PLK1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/PLK1-Antibody-3C11-Flow-Cytometry-NBP2-22196-img0001.jpg)
![Western Blot: PLK1 Antibody (3C11) [NBP2-22196] - Western blot analysis using PLK1 mAb against human PLK1 recombinant protein. (Expected MW is 45.7 kDa)](http://images.novusbio.com/fullsize/PLK1-Antibody-3C11-Western-Blot-NBP2-22196-img0005.jpg)
![Immunohistochemistry-Paraffin: PLK1 Antibody (3C11) [NBP2-22196] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PLK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PLK1-Antibody-3C11-Immunohistochemistry-Paraffin-NBP2-22196-img0002.jpg)
![ELISA: PLK1 Antibody (3C11) [NBP2-22196] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/PLK1-Antibody-3C11-ELISA-NBP2-22196-img0004.jpg)
![Western Blot: Histone H2AX [p Ser139] Antibody (9F3) [NBP1-19255] - Analysis of Histone H2AX [p Ser139] antibody (9F3) Jurkat cell lysate; Lane 3: Jurkat cell lysate treated with staurosporine; Lane 4: 3T3 cell lysate; Lane 5: CHO-K1 cell lysate; Lane 6: Rat-2 cell lysate. Bands appear at an observed molecular weight of ~15 kDa.](http://images.novusbio.com/fullsize/gamma-H2AX-p-Ser139-Antibody-9F3-Western-Blot-NBP1-19255-img0001.jpg "Western Blot: Histone H2AX [p Ser139] Antibody (9F3) [NBP1-19255] - Analysis of Histone H2AX [p Ser139] antibody (9F3) Jurkat cell lysate; Lane 3: Jurkat cell lysate treated with staurosporine; Lane 4: 3T3 cell lysate; Lane 5: CHO-K1 cell lysate; Lane 6: Rat-2 cell lysate. Bands appear at an observed molecular weight of ~15 kDa.")
![Western Blot: H2AX Antibody [NBP2-27427] - Analysis of MOLT4 lysate in the 1) absence, 2) presence of immunizing peptide using Histone H2A.x antibody at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/H2AX-Antibody-Western-Blot-NBP2-27427-img0003.jpg "Western Blot: H2AX Antibody [NBP2-27427] - Analysis of MOLT4 lysate in the 1) absence, 2) presence of immunizing peptide using Histone H2A.x antibody at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: H2AX Antibody [NBP2-27427] - Formalin-fixed, paraffin-embedded human spleen stained with Histone H2A.x antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/H2AX-Antibody-Immunohistochemistry-Paraffin-NBP2-27427-img0004.jpg)
![Western Blot: HMG14 Antibody [NBP2-14842] - Analysis of HMG14 in Daudi lysate in the 1) absence and 2) presence of immunizing peptide probed with 2 ug/ml of HMG14 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/HMG14-Antibody-Western-Blot-NBP2-14842-img0003.jpg "Western Blot: HMG14 Antibody [NBP2-14842] - Analysis of HMG14 in Daudi lysate in the 1) absence and 2) presence of immunizing peptide probed with 2 ug/ml of HMG14 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: PUMA Antibody (9C5W5) [NBP3-16268] - Analysis of extracts of various cell lines, using PUMA Rabbit mAb (NBP3-16268) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/PUMA-Antibody-9C5W5-Western-Blot-NBP3-16268-img0006.jpg "Western Blot: PUMA Antibody (9C5W5) [NBP3-16268] - Analysis of extracts of various cell lines, using PUMA Rabbit mAb (NBP3-16268) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: PUMA Antibody (9C5W5) [NBP3-16268] - Mouse spinal cord using PUMA Rabbit mAb (NBP3-16268) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PUMA-Antibody-9C5W5-Immunohistochemistry-Paraffin-NBP3-16268-img0002.jpg)
![Immunohistochemistry-Paraffin: PUMA Antibody (9C5W5) [NBP3-16268] - Rat brain using PUMA Rabbit mAb (NBP3-16268) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PUMA-Antibody-9C5W5-Immunohistochemistry-Paraffin-NBP3-16268-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: PUMA Antibody (9C5W5) [NBP3-16268] - Confocal imaging of NIH/3T3 cells using PUMA Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16268_rabbit-puma-mab-9c5w5-5220251265370.jpg)
![Immunocytochemistry/ Immunofluorescence: PUMA Antibody (9C5W5) [NBP3-16268] - Confocal imaging of U-2 OS cells using PUMA Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16268_rabbit-puma-mab-9c5w5-522025125414.jpg)
![Western Blot: p53 DINP1 Antibody (9Y6Z7) [NBP3-16620] - Western blot analysis of extracts of various cell lines, using p53 DINP1 Rabbit mAb (NBP3-16620) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/p53-DINP1-Antibody-9Y6Z7-Western-Blot-NBP3-16620-img0003.jpg "Western Blot: p53 DINP1 Antibody (9Y6Z7) [NBP3-16620] - Western blot analysis of extracts of various cell lines, using p53 DINP1 Rabbit mAb (NBP3-16620) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: p53 DINP1 Antibody (9Y6Z7) [NBP3-16620] - Immunofluorescence analysis of HeLa cells using p53 DINP1 Rabbit mAb (NBP3-16620) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/p53-DINP1-Antibody-9Y6Z7-Immunocytochemistry-Immunofluorescence-NBP3-16620-img0002.jpg)
![Immunohistochemistry-Paraffin: p53 DINP1 Antibody (9Y6Z7) [NBP3-16620] - Immunohistochemistry of paraffin-embedded human liver using p53 DINP1 Rabbit mAb (NBP3-16620) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/p53-DINP1-Antibody-9Y6Z7-Immunohistochemistry-Paraffin-NBP3-16620-img0001.jpg)
![Western Blot: p53 DINP1 Antibody (9Y6Z7) [NBP3-16620] - Western blot analysis of extracts of various cell lines, using p53 DINP1 Rabbit mAb (NBP3-16620) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/p53-DINP1-Antibody-9Y6Z7-Western-Blot-NBP3-16620-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: p53 DINP1 Antibody (9Y6Z7) [NBP3-16620] - Confocal imaging of HeLa cells using p53 DINP1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16620_rabbit-p53-dinp1-mab-9y6z7-52202511392239.jpg)
![Western Blot: Ku70/XRCC6 Antibody (7A9E7) [NBP2-52590] - Analysis using XRCC6 mAb against human XRCC6 (AA: 6-214) recombinant protein. (Expected MW is 49.7 kDa)](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-7A9E7-Western-Blot-NBP2-52590-img0003.jpg "Western Blot: Ku70/XRCC6 Antibody (7A9E7) [NBP2-52590] - Analysis using XRCC6 mAb against human XRCC6 (AA: 6-214) recombinant protein. (Expected MW is 49.7 kDa)")
![Immunocytochemistry/Immunofluorescence: Ku70/XRCC6 Antibody (7A9E7) [NBP2-52590] - Analysis of MCF-7 cells using XRCC6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-7A9E7-Immunofluorescence-NBP2-52590-img0002.jpg)
![Flow Cytometry: Ku70/XRCC6 Antibody (7A9E7) [NBP2-52590] - Analysis of A431 cells using XRCC6 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-7A9E7-Flow-Cytometry-NBP2-52590-img0001.jpg)
![Western Blot: Ku70/XRCC6 Antibody (7A9E7) [NBP2-52590] - Analysis using XRCC6 mAb against HEK293 (1) and XRCC6 (AA: 6-214)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-7A9E7-Western-Blot-NBP2-52590-img0004.jpg)
![ELISA: Ku70/XRCC6 Antibody (7A9E7) [NBP2-52590] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-7A9E7-ELISA-NBP2-52590-img0006.jpg)
![Ku70/XRCC6 Antibody (7A9E7) [NBP2-52590] - Analysis using XRCC6 mouse mAb against PC-2 (1), A549 (2), A431 (3), HepG2 (4), K562 (5) cell lysate.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-7A9E7-NBP2-52590-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: RPA2 Antibody (12F3.3) [NB100-158] - 2mM hydroxyurea treated (right) or untreated (left) HeLa cells were fixed in ice-cold MeOH for 5 min. Green: RPA32 protein stained by RPA32 antibody [12F3.3] Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/RPA2-Antibody-12F3-3-Immunocytochemistry-Immunofluorescence-NB100-158-img0003.jpg "<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: RPA2 Antibody (12F3.3) [NB100-158] - 2mM hydroxyurea treated (right) or untreated (left) HeLa cells were fixed in ice-cold MeOH for 5 min. Green: RPA32 protein stained by RPA32 antibody [12F3.3] Blue: Hoechst 33342 staining.")
![<b>Biological Strategies Validation. </b>Western Blot: RPA2 Antibody (12F3.3) [NB100-158] - Various whole cell extracts (30 ug) were separated by 12 % SDS-PAGE, and blotted with RPA32 antibody diluted by 1:500](http://images.novusbio.com/fullsize/RPA2-Antibody-12F3-3-Western-Blot-NB100-158-img0004.jpg)
![Western Blot: p53 DINP1 Antibody [NB100-56627] - analysis of p53DINP1 expression in mouse spleen using this antibody.](http://images.novusbio.com/fullsize/p53-DINP1-Antibody-Western-Blot-NB100-56627-img0002.jpg "Western Blot: p53 DINP1 Antibody [NB100-56627] - analysis of p53DINP1 expression in mouse spleen using this antibody.")
![Western Blot: PLK1 Antibody [NB100-547] - Detection of Human PLK1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PLK1 antibody NB100-547 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PLK1-Antibody-Western-Blot-NB100-547-img0021.jpg "Western Blot: PLK1 Antibody [NB100-547] - Detection of Human PLK1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PLK1 antibody NB100-547 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunocytochemistry/Immunofluorescence: PLK1 Antibody [NB100-547] - Detection of human PLK1 by immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti- PLK1 (NB100-547). Detection: Green fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody FITC conjugated.](http://images.novusbio.com/fullsize/PLK1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-547-img0022.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: PLK1 Antibody [NB100-547] - Detection of Human PLK1 on HeLa whole cell lysate using NB100-547. B. Affinity purified rabbit anti-PLK1 antibodies NB100-546, NB100-547, and NB100-548. IPed PLK1 was detected using NB100-548.](http://images.novusbio.com/fullsize/PLK1-Antibody-Western-Blot-NB100-547-img0020.jpg)
![Western Blot: FANCD2 Antibody (1290C) [NBP2-61930] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FANCD2 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/mL Rabbit Monoclonal FANCD2 Antibody (1290C) (Catalog # NBP2-61930) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FANCD2 at approximately 150 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/FANCD2-Antibody-1290C-Knockout-Validated-NBP2-61930-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: FANCD2 Antibody (1290C) [NBP2-61930] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FANCD2 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/mL Rabbit Monoclonal FANCD2 Antibody (1290C) (Catalog # NBP2-61930) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FANCD2 at approximately 150 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: FANCD2 Antibody (1290C) [NBP2-61930] - Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line (Molecular weight: 164.1 KDa), Hepa 1-6 mouse hepatoma cell line, and H4-II-E-C3 rat hepatoma cell line. PVDF membrane was probed with 0.5 ug/mL of Rabbit Monoclonal FANCD2 Antibody (1290C) (Catalog # NBP2-61930) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for FANCD2 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/FANCD2-Antibody-1290C-Western-Blot-NBP2-61930-img0004.jpg)
![Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021] - Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates transfected with various plk-1 mutation constructs. Blots were reacted with anti-Plk-1 pT210 (panel A) and pan reactive anti-Plk-1 (panel B). Transfected cells were treated with 1 uM nocodazole followed by cell collection, lysate preparation, SDS-PAGE and western blotting. Using a 1:1000 dilution, anti-Plk-1 pT210 detects a single band corresponding to endogenous plk-1, but does not detect recombinant forms of the protein presumably because of a lack of phosphorylation in these mutants.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Western-Blot-NBP1-78021-img0005.jpg "Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021] - Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates transfected with various plk-1 mutation constructs. Blots were reacted with anti-Plk-1 pT210 (panel A) and pan reactive anti-Plk-1 (panel B). Transfected cells were treated with 1 uM nocodazole followed by cell collection, lysate preparation, SDS-PAGE and western blotting. Using a 1:1000 dilution, anti-Plk-1 pT210 detects a single band corresponding to endogenous plk-1, but does not detect recombinant forms of the protein presumably because of a lack of phosphorylation in these mutants.")
![Immunohistochemistry: PLK1 [p Thr210] Antibody [NBP1-78021] - Used at a 1:200 dilution to detect Plk1 by immunohistochemistry in human breast carcinoma tumor tissue. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Immunohistochemistry-NBP1-78021-img0006.jpg)
![Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021] - Plk-1 pT210 antibody to detect endogenous protein present in a Mouse A20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~68 kDa corresponding to Plk-1 protein. It is suggested to use a nuclear extract from synchronized cells to greatly increase the abundance of this protein in preparations. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Western-Blot-NBP1-78021-img0002.jpg)
![Immunohistochemistry: PLK1 [p Thr210] Antibody [NBP1-78021] - Used at a 1:200 dilution to detect Plk1 by immunohistochemistry in human colon carcinoma tumor tissue. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Immunohistochemistry-NBP1-78021-img0007.jpg)
