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![Western Blot: Cyclin A2 Antibody (E67) [NBP2-34311] - Western Blot Analysis of HeLa cell lysate using Cyclin A2 Antibody (E67).](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-E67-Western-Blot-NBP2-34311-img0003.jpg "Western Blot: Cyclin A2 Antibody (E67) [NBP2-34311] - Western Blot Analysis of HeLa cell lysate using Cyclin A2 Antibody (E67).")
![Immunohistochemistry-Paraffin: Cyclin A2 Antibody (E67) [NBP2-34311] - Formalin-fixed, paraffin-embedded human Endometrium stained with Cyclin A2 Antibody (E67).](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-E67-Immunohistochemistry-Paraffin-NBP2-34311-img0002.jpg)
![Western Blot: Aurora A Antibody [NBP2-36737] - Analysis of Aurora Kinase A protein in lysates of (A) Human HCT-116 cell line, (B) Human testis, (C) Mouse testis, and (D) Rat testis. Aurora A antibody used at 2 ug/ml concentration.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Western-Blot-NBP2-36737-img0001.jpg "Western Blot: Aurora A Antibody [NBP2-36737] - Analysis of Aurora Kinase A protein in lysates of (A) Human HCT-116 cell line, (B) Human testis, (C) Mouse testis, and (D) Rat testis. Aurora A antibody used at 2 ug/ml concentration.")
![Immunohistochemistry-Paraffin: Aurora A Antibody [NBP2-36737] - Analysis of FFPE section of human testis using Aurora A antibody at 10 ug/ml concentration. Heat-induced antigen retrieval was employed in this IHC-P validation.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunohistochemistry-Paraffin-NBP2-36737-img0002.jpg)
![Western Blot: ErbB2/HER2 Antibody [NBP2-29624] - Western blot analysis of ErbB2/HER2 in SKOV3 cell lysate.](http://images.novusbio.com/fullsize/ErbB2-HER2-Antibody-Western-Blot-NBP2-29624-img0001.jpg "Western Blot: ErbB2/HER2 Antibody [NBP2-29624] - Western blot analysis of ErbB2/HER2 in SKOV3 cell lysate.")
![Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP2-29624] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast carcinoma tissue section using 1:50 dilution of ErbB2/HER2 antibody (NBP2-29624) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This ErbB3 antibody generated a diffused cytoplasmic with some membrane and nuclear staining in the cancer cells, endothelial cells as well as in the tumor stromal cells. The cells in the cores of some tumor areas were negative for ErbB2.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-Immunohistochemistry-Paraffin-NBP2-29624-img0003.jpg)
![Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP2-29624] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast carcinoma tissue section using 1:50 dilution of ErbB2/HER2 antibody (NBP2-29624) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This ErbB2 antibody generated a diffused cytoplasmic with some membrane and nuclear staining in the cancer cells, endothelial cells as well as in the tumor stromal cells. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-Immunohistochemistry-Paraffin-NBP2-29624-img0002.jpg)
![Western Blot: Aurora B Antibody (3F11) [NBP2-50039] - Analysis of different cell lysates and recombinant protein solutions using mouse mAb to Aurora B. Left: cells were treated with 100ng/mL of nocodazol, a microtubule depolymerizing agent which induces cells to halt at G2/M phase. [1] protein standard, [2] HeLa, [3] canine A72 cells, [4] equine NBL6 cells, and [5] mouse KR158 cells. Right: Blot of purified full length recombinant human aurora A, B and C were probed with NBP2-50039. The antibody binds specifically only to aurora B and not to the closely related aurora A and C.](http://images.novusbio.com/fullsize/Aurora-B-Antibody-3F11-Western-Blot-NBP2-50039-img0006.jpg "Western Blot: Aurora B Antibody (3F11) [NBP2-50039] - Analysis of different cell lysates and recombinant protein solutions using mouse mAb to Aurora B. Left: cells were treated with 100ng/mL of nocodazol, a microtubule depolymerizing agent which induces cells to halt at G2/M phase. [1] protein standard, [2] HeLa, [3] canine A72 cells, [4] equine NBL6 cells, and [5] mouse KR158 cells. Right: Blot of purified full length recombinant human aurora A, B and C were probed with NBP2-50039. The antibody binds specifically only to aurora B and not to the closely related aurora A and C.")
![Immunocytochemistry/Immunofluorescence: Aurora B Antibody (3F11) [NBP2-50039] - High magnification confocal immunofluorescence of HeLa cells stained with NBP2-50039, diluted 1:1,000 in green and counterstained with antibody to vimentin at 1:1,000 dilution in red. Blue is the DNA stain DAPI revealing the nuclei. The MCA-3F11 reveals aurora B localized in midbodies, midzones of dividing cells and also in the nuclei or some cells.](http://images.novusbio.com/fullsize/Aurora-B-Antibody-3F11-Immunocytochemistry-Immunofluorescence-NBP2-50039-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Aurora B Antibody (3F11) [NBP2-50039] - HeLa cell cultures were stained with NBP2-50039 (green). Aurora B stains midzones in anaphase and midbodies between the two daughter cells during telophase. It is therefore a useful marker of dividing cells. Cells were counterstained with chicken polyclonal antibody to Vimentin (NB300-223, red). Blue is a DNA stain.](http://images.novusbio.com/fullsize/Aurora-B-Antibody-3F11-Immunocytochemistry-Immunofluorescence-NBP2-50039-img0003.jpg)
![Western Blot: EEF1E1 Antibody [NBP1-32184] - Sample(30 ug of whole cell lysate)A:A431 12% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/EEF1E1-Antibody-Western-Blot-NBP1-32184-img0004.jpg "Western Blot: EEF1E1 Antibody [NBP1-32184] - Sample(30 ug of whole cell lysate)A:A431 12% SDS PAGE, antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: EEF1E1 Antibody [NBP1-32184] - Analysis of paraformaldehyde-fixed HeLa, using EEF1E1 antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/EEF1E1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32184-img0005.jpg)
![Immunohistochemistry-Paraffin: EEF1E1 Antibody [NBP1-32184] - Mouse fore brain. EEF1E1 antibody [N1C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/EEF1E1-Antibody-Immunohistochemistry-Paraffin-NBP1-32184-img0007.jpg)
![Western Blot: EEF1E1 Antibody [NBP1-32184] - Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/EEF1E1-Antibody-Western-Blot-NBP1-32184-img0003.jpg)
![Immunohistochemistry-Paraffin: EEF1E1 Antibody [NBP1-32184] - Mouse fore brain. EEF1E1 antibody [N1C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/EEF1E1-Antibody-Immunohistochemistry-Paraffin-NBP1-32184-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: EEF1E1 Antibody [NBP1-32184] - Wild-type (WT) and EEF1E1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with EEF1E1 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EEF1E1-Antibody-Western-Blot-NBP1-32184-img0008.jpg)
![Western Blot: PLK1 Antibody [NB100-56651] - Analysis of HCT-116 lysate using PLK1 antibody at 1:2000.](http://images.novusbio.com/fullsize/PLK1-Antibody-Western-Blot-NB100-56651-img0005.jpg "Western Blot: PLK1 Antibody [NB100-56651] - Analysis of HCT-116 lysate using PLK1 antibody at 1:2000.")
![Immunohistochemistry-Paraffin: PLK1 Antibody [NB100-56651] - Analysis of human placenta using PLK1 antibody at 1:100.](http://images.novusbio.com/fullsize/PLK1-Antibody-Immunohistochemistry-Paraffin-NB100-56651-img0004.jpg)
![Western Blot: FGF R1 [p Tyr651, p Tyr652] Antibody [NBP2-24462] - Analysis of FGFR phosphoin recombinant fusion protein containing 1) phosphorylated tyrosine residues at positions 651 and 652 and 2) unphosphorylated tyrosine residues at positions 651 and 652, using FGFR phospho antibody at 0.002 ug/ml. Goat anti-rabbit IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/FGF-R1-[p-Tyr651--p-Tyr652]-Antibody-Western-Blot-NBP2-24462-img0005.jpg "Western Blot: FGF R1 [p Tyr651, p Tyr652] Antibody [NBP2-24462] - Analysis of FGFR phosphoin recombinant fusion protein containing 1) phosphorylated tyrosine residues at positions 651 and 652 and 2) unphosphorylated tyrosine residues at positions 651 and 652, using FGFR phospho antibody at 0.002 ug/ml. Goat anti-rabbit IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: FGF R1 [p Tyr651, p Tyr652] Antibody [NBP2-24462] - Analysis of FGFR1 phospho in formalin-fixed, paraffin-embedded human breast tumor tissue using an isotype control antibody (top left) and FGFR1 phospho antibody (bottom left, right) at 5 ug/ml.](http://images.novusbio.com/fullsize/FGF-R1-[p-Tyr651--p-Tyr652]-Antibody-Immunohistochemistry-Paraffin-NBP2-24462-img0004.jpg)
![Western Blot: CHAF1A Antibody [NB100-74608] - RECQ5 promotes SUMO2 conjugation of PCNA. Western blot analysis of the indicated proteins in WCE prepared from control or RECQ5 siRNA knockdown HEK293T cells with exogenous overexpression of indicated FLAG-tagged PCNA constructs or CAF1 (CHAF1A) or treated with an empty vector (V). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-05236-y), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CHAF1A-Antibody-Western-Blot-NB100-74608-img0012.jpg "Western Blot: CHAF1A Antibody [NB100-74608] - RECQ5 promotes SUMO2 conjugation of PCNA. Western blot analysis of the indicated proteins in WCE prepared from control or RECQ5 siRNA knockdown HEK293T cells with exogenous overexpression of indicated FLAG-tagged PCNA constructs or CAF1 (CHAF1A) or treated with an empty vector (V). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-05236-y), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: CHAF1A Antibody [NB100-74608] - Human colon carcinoma peyer's patch. Antibody used at a dilution of 1:200 (1ug/ml).](http://images.novusbio.com/fullsize/CHAF1A-Antibody-Immunohistochemistry-Paraffin-NB100-74608-img0010.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: CHAF1A Antibody [NB100-74608] - Detection of Human Caf1p150 on HeLa whole cell lysate using NB100-74608. Caf1p150 was also immunoprecipitated by rabbit anti-Caf1p150 antibodies NB100-74609 and NB100-74610.](http://images.novusbio.com/fullsize/CHAF1A-Antibody-Western-Blot-NB100-74608-img0011.jpg)
![Western Blot: CHAF1A Antibody [NB100-74608] - SUMO2-PCNA enriches CAF1 & FACT within the replication complex. a Western blot analysis of the indicated proteins in the CB fractions (left) & the FLAG-PCNA complexes purified from CB fractions (right) prepared from cells expressing the indicated FLAG-tagged proteins. Ub-KR indicates the FLAG-ubiquitin-PCNA (KR) fusion protein. b Western blot analysis of the indicated proteins in the CB input (left panels) used for the in vitro pull-down experiments of purified recombinant strep-tagged PCNA, SUMO2-conjugated PCNA, & GFP anchored to Strep-Tactin resins (right panels). SUMO2-modified PCNA was generated by the in vitro SUMOylation reaction using strep-PCNA & His-SUMO2 shown in Supplementary Fig. 6a, b. c Western blot analysis of the input levels of recombinant strep-PCNA & strep-SUMO2-PCNA fusion (S2-PCNA) purified from E. coli (left panel) & of strep-PCNA & strep-S2-PCNA pulled down by WT or SIM mutant FLAG-CAF1A proteins purified & anchored on FLAG M2 agarose beads from HEK293T cells expressing the corresponding constructs (right panels). d Western blot analysis of strep-S2-PCNA (upper 2 panels) & strep-PCNA (lower 2 panels) pulled down by FLAG-CAF1A purified from E. coli & anchored on FLAG M2 agarose beads. e Western blot analysis of the indicated proteins in the CB input prepared from HEK293T cells expressing FLAG-SSRP1 WT or SIM mutant (left panels) & of FLAG-SSRP1 & SPT16 pulled down from the CB fractions in vitro using purified recombinant strep-tagged PCNA, S2-PCNA, & GFP anchored to Strep-Tactin resins (right panels) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30006506), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74608_rabbit-polyclonal-chaf1a-antibody-310202416575.jpg)
![Western Blot: BubR1 Antibody [NB100-579] - Detection of Mouse and Rat BubR1 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody NB100-579 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/BubR1-Antibody-Western-Blot-NB100-579-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: BubR1 Antibody [NB100-579] - Detection of Mouse and Rat BubR1 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody NB100-579 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: BubR1 Antibody [NB100-579] - Detection of Human BubR1 on HeLa whole cell lysate using NB100-579. BubR1 was also immunoprecipitated using another affinity purified rabbit anti-BubR1 antibody to a different epitope.](http://images.novusbio.com/fullsize/BubR1-Antibody-Western-Blot-NB100-579-img0002.jpg)
![Western Blot: BubR1 Antibody [NB100-579] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody NB100-579 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.](http://images.novusbio.com/fullsize/BubR1-Antibody-Western-Blot-NB100-579-img0001.jpg)
![Western Blot: eIF4B Antibody [NB100-93308] - Detection of Human eIF4B on HeLa whole cell lysate using NB100-93308. eIF4B was also immunoprecipitated by rabbit anti-eIF4B antibody NB100-93307.](http://images.novusbio.com/fullsize/eIF4B-Antibody-Western-Blot-NB100-93308-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: eIF4B Antibody [NB100-93308] - Detection of Human eIF4B on HeLa whole cell lysate using NB100-93308. eIF4B was also immunoprecipitated by rabbit anti-eIF4B antibody NB100-93307.")
![Western Blot: Peroxiredoxin 3 Antibody [NBP2-19777] - Wild-type (WT) and Peroxiredoxin 3 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Peroxiredoxin 3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Peroxiredoxin-3-Antibody-Western-Blot-NBP2-19777-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Peroxiredoxin 3 Antibody [NBP2-19777] - Wild-type (WT) and Peroxiredoxin 3 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Peroxiredoxin 3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Peroxiredoxin 3 Antibody [NBP2-19777] - Immunohistochemical analysis of paraffin-embedded Lung AdC, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Peroxiredoxin-3-Antibody-Immunohistochemistry-Paraffin-NBP2-19777-img0003.jpg)
![Western Blot: Peroxiredoxin 3 Antibody [NBP2-19777] - Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 C: Molt-4 D: Raji 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Peroxiredoxin-3-Antibody-Western-Blot-NBP2-19777-img0001.jpg)
![Western Blot: Peroxiredoxin 3 Antibody [NBP2-19777] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Peroxiredoxin-3-Antibody-Western-Blot-NBP2-19777-img0002.jpg)
![Peroxiredoxin 3 antibody [N1C3] detects Peroxiredoxin 3 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.Green: Peroxiredoxin 3 protein stained by Peroxiredoxin 3 antibody [N1C3] (NBP2-19777) diluted at 1:1000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-19777_rabbit-polyclonal-peroxiredoxin-3-antibody-immunocytochemistry-immunofluorescence-261120251840485.jpg)
![Immunohistochemistry-Paraffin: Wee1 Antibody [NBP1-33506] - CL1-0 Xenograft, using WEE1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Wee1-Antibody-Immunohistochemistry-Paraffin-NBP1-33506-img0003.jpg "Immunohistochemistry-Paraffin: Wee1 Antibody [NBP1-33506] - CL1-0 Xenograft, using WEE1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: Wee1 Antibody [NBP1-33506] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with WEE1 antibody [N3C2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Wee1-Antibody-Western-Blot-NBP1-33506-img0007.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with WEE1 antibody [N3C2], Internal (NBP1-33506) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33506_rabbit-polyclonal-wee1-antibody-western-blot-2611202518191318.jpg)
![Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with WEE1 antibody [N3C2], Internal (NBP1-33506) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33506_rabbit-polyclonal-wee1-antibody-western-blot-261120251830124.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with WEE1 antibody [N3C2], Internal (NBP1-33506) diluted at 1:1000 and competitor's antibody (CST#13084) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33506_rabbit-polyclonal-wee1-antibody-western-blot-261120251829022.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with WEE1 antibody [N3C2], Internal (NBP1-33506) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-33506_rabbit-polyclonal-wee1-antibody-western-blot-26112025196217.jpg)