| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-42822SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1062AF405 | |||
| Alexa Fluor 488 | NB21-1062AF488 | |||
| Alexa Fluor 647 | NB21-1062AF647 | |||
| Alexa Fluor 700 | NB21-1062AF700 | |||
| Biotin | NB21-1062B | |||
| DyLight 405 | NB21-1062V | |||
| DyLight 488 | NB21-1062G | |||
| DyLight 550 | NB21-1062R | |||
| DyLight 650 | NB21-1062C | |||
| FITC | NB21-1062F | |||
| HRP | NB21-1062H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1062SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1024AF405 | |||
| Alexa Fluor 488 | NB21-1024AF488 | |||
| Alexa Fluor 647 | NB21-1024AF647 | |||
| Alexa Fluor 700 | NB21-1024AF700 | |||
| Biotin | NB21-1024B | |||
| DyLight 405 | NB21-1024V | |||
| DyLight 488 | NB21-1024G | |||
| DyLight 550 | NB21-1024R | |||
| DyLight 650 | NB21-1024C | |||
| FITC | NB21-1024F | |||
| HRP | NB21-1024H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1024SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1021AF405 | |||
| Alexa Fluor 488 | NB21-1021AF488 | |||
| Alexa Fluor 647 | NB21-1021AF647 | |||
| Alexa Fluor 700 | NB21-1021AF700 | |||
| Biotin | NB21-1021B | |||
| DyLight 405 | NB21-1021V | |||
| DyLight 488 | NB21-1021G | |||
| DyLight 550 | NB21-1021R | |||
| DyLight 650 | NB21-1021C | |||
| FITC | NB21-1021F | |||
| HRP | NB21-1021H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1021SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1074AF405 | |||
| Alexa Fluor 488 | NB21-1074AF488 | |||
| Alexa Fluor 647 | NB21-1074AF647 | |||
| Alexa Fluor 700 | NB21-1074AF700 | |||
| Biotin | NB21-1074B | |||
| DyLight 405 | NB21-1074V | |||
| DyLight 488 | NB21-1074G | |||
| DyLight 550 | NB21-1074R | |||
| DyLight 650 | NB21-1074C | |||
| FITC | NB21-1074F | |||
| HRP | NB21-1074H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1074SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1002AF405 | |||
| Alexa Fluor 488 | NB21-1002AF488 | |||
| Alexa Fluor 647 | NB21-1002AF647 | |||
| Alexa Fluor 700 | NB21-1002AF700 | |||
| Biotin | NB21-1002B | |||
| DyLight 405 | NB21-1002V | |||
| DyLight 488 | NB21-1002G | |||
| DyLight 550 | NB21-1002R | |||
| DyLight 650 | NB21-1002C | |||
| FITC | NB21-1002F | |||
| HRP | NB21-1002H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1002SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1081AF405 | |||
| Alexa Fluor 488 | NB21-1081AF488 | |||
| Alexa Fluor 647 | NB21-1081AF647 | |||
| Alexa Fluor 700 | NB21-1081AF700 | |||
| Biotin | NB21-1081B | |||
| DyLight 405 | NB21-1081V | |||
| DyLight 488 | NB21-1081G | |||
| DyLight 550 | NB21-1081R | |||
| DyLight 650 | NB21-1081C | |||
| FITC | NB21-1081F | |||
| HRP | NB21-1081H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1081SS | |||
![Western Blot: p62/SQSTM1 Antibody [NBP1-42822] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p62/SQSTM1 knockout (KO) HeLa cell line. PVDF membrane was probed with Rabbit Anti-Human p62/SQSTM1 polyclonal Antibody (Catalog # NBP1-42822) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for p62/SQSTM1 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Knockout-Validated-NBP1-42822-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p62/SQSTM1 Antibody [NBP1-42822] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p62/SQSTM1 knockout (KO) HeLa cell line. PVDF membrane was probed with Rabbit Anti-Human p62/SQSTM1 polyclonal Antibody (Catalog # NBP1-42822) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for p62/SQSTM1 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: p62/SQSTM1 Antibody [NBP1-42822] - HeLa cells were treated with (+) or without 50 uM (-) of Chloriquine (CQ) for 24 hours. Total cell lysates were prepared and separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane and blocked in 5% non-fat milk. The membrane was then probed with 2 ug/ml anti-p62/SQSMT1 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the upregulation of p62 (arrowhead) in response to chloroquine treatment and the blockage of autophagy.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Western-Blot-NBP1-42822-img0008.jpg)
![Simple Western: p62/SQSTM1 Antibody [NBP1-42822] - Simple Western lane view shows a specific band for p62/SQSTM1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Simple-Western-NBP1-42822-img0007.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: p62/SQSTM1 Antibody [NBP1-42822] - Hyperosmotic stress stimulatesA utophagyHeLa and HCT 116 cells were transduced with an adenovirus coding for GFP-LC3 (Ad GFP-LC3) for 24 h. LC3 I-to-LC3 II conversion and p62/SQSTM1 depletion were assessed by Western blot analysis in HeLa and HCT116 cells treated with various concentrations of sorbitol or mannitol (25-300 mOsm) .It indicated times (0.5-6 h). Image collected and cropped by Citeab from the following publication ( licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Western-Blot-NBP1-42822-img0010.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Asym-dimethyl Arg8] Antibody [NB21-1062] - Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2ug of NB21-1062 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg8]-Antibody-Chromatin-Immunoprecipitation-NB21-1062-img0007.jpg "Chromatin Immunoprecipitation: Histone H3 [Asym-dimethyl Arg8] Antibody [NB21-1062] - Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2ug of NB21-1062 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.")
![Western Blot: Histone H3 [Asym-dimethyl Arg8] Antibody [NB21-1062] - Analysis of Histone H3R8me2 in: (1) HeLa Histone Prep Lysate, (2) NIH 3T3 Histone Prep Lysate, and (3) C. elegans Embryo Lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg8]-Antibody-Western-Blot-NB21-1062-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Asym-dimethyl Arg8] Antibody [NB21-1062] - Immunofluorescence: [NB21-1062] - Histone H3.2 R8me2a antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg8]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1062-img0011.jpg)
![Western Blot: Histone H3 [p Ser28] Antibody (HTA28) [NB600-1168] - Whole extract of HeLa cell treated with nocodazole was separated on SDS-PAGE and probed with Monoclonal Anti-phospho-Histone H3 (pSer28), Clone: HTA28. The antibody was developed using Goat Anti-Rat IgG-Peroxidase and a chemiluminescent substrate.Lanes:1. Antibody dilution 0.5 ug/mL2. Antibody dilution 1 ug/mL3. Antibody dilution 2 ug/mL](http://images.novusbio.com/fullsize/Histone-H3-p-Ser28-Antibody-HTA28-Western-Blot-NB600-1168-img0008.jpg "Western Blot: Histone H3 [p Ser28] Antibody (HTA28) [NB600-1168] - Whole extract of HeLa cell treated with nocodazole was separated on SDS-PAGE and probed with Monoclonal Anti-phospho-Histone H3 (pSer28), Clone: HTA28. The antibody was developed using Goat Anti-Rat IgG-Peroxidase and a chemiluminescent substrate.Lanes:1. Antibody dilution 0.5 ug/mL2. Antibody dilution 1 ug/mL3. Antibody dilution 2 ug/mL")
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Ser28] Antibody (HTA28) [NB600-1168] - HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 5 ug/mL Monoclonal Anti-phospho-Histone H3 (pSer28), Clone: HTA28. The antibody was developed using Goat Anti-Rat IgG, FITC conjugate (green). Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/Histone-H3-p-Ser28-Antibody-HTA28-Immunocytochemistry-Immunofluorescence-NB600-1168-img0007.jpg)
![Flow Cytometry: Histone H3 [p Ser28] Antibody (HTA28) [NB600-1168] - FACS profile of human leukemic cells with Anti-phospho-Histone H3.](http://images.novusbio.com/fullsize/Histone-H3-p-Ser28-Antibody-HTA28-Flow-Cytometry-NB600-1168-img0006.jpg)
![Dot Blot: Histone H3 [ac Lys4] Antibody [NB21-1024] - Analysis of Histone H3 K4ac antibody in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys4]-Antibody-Dot-Blot-NB21-1024-img0015.jpg "Dot Blot: Histone H3 [ac Lys4] Antibody [NB21-1024] - Analysis of Histone H3 K4ac antibody in picomoles of peptide.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys4] Antibody [NB21-1024] - Histone H3 [ac Lys4] Antibody [NB21-1024] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1024 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys4]-Antibody-Chromatin-Immunoprecipitation-NB21-1024-img0019.jpg)
![Western Blot: Histone H3 [ac Lys4] Antibody [NB21-1024] - Analysis of Histone H3 K4ac in C. elegans embryo lysate. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys4]-Antibody-Western-Blot-NB21-1024-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys4] Antibody [NB21-1024] - Histone H3 K4ac1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys4]-Antibody-Chromatin-Immunoprecipitation-NB21-1024-img0021.jpg)
![Western Blot: Histone H3 [ac Lys4] Antibody [NB21-1024] - Analysis of Histone H3 K4ac in (1) HeLa histone prep and (2) NIH 3T3 histone prep lysates. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys4]-Antibody-Western-Blot-NB21-1024-img0016.jpg)
![Western Blot: Histone H3 [Monomethyl Lys4] Antibody [NB21-1021] - Analysis of Histone H3K4me1 in HeLa histone prep lysate. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys4]-Antibody-Western-Blot-NB21-1021-img0002.jpg "Western Blot: Histone H3 [Monomethyl Lys4] Antibody [NB21-1021] - Analysis of Histone H3K4me1 in HeLa histone prep lysate. Observed molecular weight is ~16 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Monomethyl Lys4] Antibody [NB21-1021] - Histone H3.2 K4me1 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys4]-Antibody-Immunocytochemistry-NB21-1021-img0001.jpg)
![Western Blot: Histone H3 [Monomethyl Lys4] Antibody [NB21-1021] - H3K4Me1 immunoblot in Jurkat cells. Observed molecular weight is ~17 kDa. Image from verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys4]-Antibody-Western-Blot-NB21-1021-img0006.jpg)
![Western Blot: Histone H3 [Monomethyl Lys4] Antibody [NB21-1021] - Analysis of H3K4me1 in C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys4]-Antibody-Western-Blot-NB21-1021-img0003.jpg)
![Dot Blot: Histone H3 [ac Lys9] Antibody [NB21-1074] - Analysis of Histone H3 K9 acetyl in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Dot-Blot-NB21-1074-img0011.jpg "Dot Blot: Histone H3 [ac Lys9] Antibody [NB21-1074] - Analysis of Histone H3 K9 acetyl in picomoles of peptide.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys9] Antibody [NB21-1074] - 2 ug of NB21-1074 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Chromatin-Immunoprecipitation-NB21-1074-img0014.jpg)
![Western Blot: Histone H3 [ac Lys9] Antibody [NB21-1074] - Analysis of H3K9ac in C. elegans embryo lysate. Observed molecular weight is ~12 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Western-Blot-NB21-1074-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys9] Antibody [NB21-1074] - Histone H3.2 K9ac antibody was tested at 1:100 in HeLa cells with FITC (green).](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1074-img0018.jpg)
![Western Blot: Histone H3 [ac Lys9] Antibody [NB21-1074] - Analysis of Histone H3 K9ac in HeLa histone prep. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Western-Blot-NB21-1074-img0016.jpg)
![Western Blot: Histone H3 [ac Lys9] Antibody [NB21-1074] - Analysis of Histone H3 K9ac in NIH/3T3 histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Western-Blot-NB21-1074-img0012.jpg)
![Western Blot: p62/SQSTM1 Antibody [NBP1-49954] - Human AC16 under normoxic and hypoxic conditions. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Western-Blot-NBP1-49954-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: p62/SQSTM1 Antibody [NBP1-49954] - Human AC16 under normoxic and hypoxic conditions. WB image submitted by a verified customer review.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: p62/SQSTM1 Antibody [NBP1-49954] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Sequestosome-1 used at a dilution of 1:1,000 (1ug/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Immunohistochemistry-NBP1-49954-img0005.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: p62/SQSTM1 Antibody [NBP1-49954] - Detection of Human and Mouse Sequestosome-1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Sequestosome-1 antibody used for WB at 0.1 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Sequestosome-1 was also immunoprecipitated by rabbit anti-Sequestosome-1 antibodies which recognize downstream epitopes. For blotting immunoprecipitated Sequestosome-1 was used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Western-Blot-NBP1-49954-img0004.jpg)
![Simple Western: p62/SQSTM1 Antibody [NBP1-49954] - Simple Western lane view shows a specific band for p62/SQSTM1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Simple-Western-NBP1-49954-img0003.jpg)
![Western Blot: BAF180/PB1 Antibody [NB100-79833] - Whole cell lysate (50 ug) from NIH 3T3 and TCMK-1 prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PBRM1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-Western-Blot-NB100-79833-img0012.jpg "Western Blot: BAF180/PB1 Antibody [NB100-79833] - Whole cell lysate (50 ug) from NIH 3T3 and TCMK-1 prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PBRM1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry-Paraffin: BAF180/PB1 Antibody [NB100-79833] - FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-PBRM1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-Immunohistochemistry-Paraffin-NB100-79833-img0014.jpg)
![Western Blot: BAF180/PB1 Antibody [NB100-79833] - Whole cell lysate (50 ug) from Jurkat, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PBRM1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-Western-Blot-NB100-79833-img0011.jpg)
![Immunohistochemistry-Paraffin: BAF180/PB1 Antibody [NB100-79833] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PBRM1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-Immunohistochemistry-Paraffin-NB100-79833-img0013.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: BAF180/PB1 Antibody [NB100-79833] - Detection of human PBRM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PBRM1 antibody NB100-79833 used for IP at 3 ug per reaction. PBRM1 was also immunoprecipitated by rabbit anti-PBRM1 antibody NB100-79832. For blotting immunoprecipitated PBRM1, NB100-79833 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-Immunoprecipitation-NB100-79833-img0010.jpg)
![Dot Blot: Histone H3 [Asym-dimethyl Arg2] Antibody [NB21-1002] - Analysis of Histone H3 [Asym-dimethyl Arg2] in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg2]-Antibody-Dot-Blot-NB21-1002-img0007.jpg "Dot Blot: Histone H3 [Asym-dimethyl Arg2] Antibody [NB21-1002] - Analysis of Histone H3 [Asym-dimethyl Arg2] in picomoles of peptide.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Asym-dimethyl Arg2] Antibody [NB21-1002] - Histone H3 [Asym-dimethyl Arg2] antibody was tested in HeLa cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg2]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1002-img0006.jpg)
![Western Blot: Histone H3 [Asym-dimethyl Arg2] Antibody [NB21-1002] - Analysis of Histone H3 [Asym-dimethyl Arg2] in C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg2]-Antibody-Western-Blot-NB21-1002-img0008.jpg)
![Dot Blot: Histone H3 [ac Lys9, ac Lys14] Antibody [NB21-1081] - Dot blot analysis of H3 K36ac in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-Dot-Blot-NB21-1081-img0008.jpg "Dot Blot: Histone H3 [ac Lys9, ac Lys14] Antibody [NB21-1081] - Dot blot analysis of H3 K36ac in picomoles of peptide.")
![Western Blot: Histone H3 [ac Lys9, ac Lys14] Antibody [NB21-1081] - WB analysis of H3K9ac/K14ac in C. elegans embryo lysate. Observed molecular weight is ~11 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-Western-Blot-NB21-1081-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys9, ac Lys14] Antibody [NB21-1081] - Histone H3.2 K9,K14ac antibody was tested at 1:50 in HeLa cells with FITC. Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-Immunocytochemistry-NB21-1081-img0012.jpg)
![Flow Cytometry: Histone H3 [ac Lys9, ac Lys14] Antibody [NB21-1081] - Analysis of Alexa Fluor (R) 700 conjugate of NB21-1081. Jurkat cells stained with Alexa Fluor 700 conjugated acetylated Histone H3 antibody (blue) or isotype control (red). Image from verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-Flow-Cytometry-NB21-1081-img0013.jpg)
![Western Blot: Histone H3 [ac Lys9, ac Lys14] Antibody [NB21-1081] - WB analysis of H3 K9ac/K14ac in NIH/3T3 histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-Western-Blot-NB21-1081-img0009.jpg)
![Western Blot: Brg1 Antibody [NB100-2594] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRG1/SMARCA4 antibodyused for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30seconds.](http://images.novusbio.com/fullsize/Brg1-Antibody-Western-Blot-NB100-2594-img0019.jpg "Western Blot: Brg1 Antibody [NB100-2594] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRG1/SMARCA4 antibodyused for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30seconds.")
![Immunohistochemistry-Paraffin: Brg1 Antibody [NB100-2594] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit antiBRG1/SMARCA4 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Brg1-Antibody-Immunohistochemistry-Paraffin-NB100-2594-img0017.jpg)
![Western Blot: Brg1 Antibody [NB100-2594] - Whole cell lysate (15 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRG1/SMARCA4 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Brg1-Antibody-Western-Blot-NB100-2594-img0018.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody [NB100-2594] - Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit antiBRG1/SMARCA4 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Brg1-Antibody-Immunohistochemistry-Paraffin-NB100-2594-img0016.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Brg1 Antibody [NB100-2594] - Detection of human BRG1-SMARCA4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRG1-SMARCA4 antibody NB100-2594 used for IP at 3 ug per reaction. BRG1-SMARCA4 was also immunoprecipitated by rabbit anti-BRG1-SMARCA4 antibody NBP-22234. For blotting immunoprecipitated BRG1-SMARCA4, NB100-2594 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Brg1-Antibody-Immunoprecipitation-NB100-2594-img0020.jpg)
![Western Blot: CHD1 Antibody [NB100-60411] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and CHD1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:2000 of Rabbit Anti-Human CHD1 Polyclonal Antibody (Catalog # NB100-60411) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for CHD1 at approximately 260 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/CHD1-Antibody-Knockout-Validated-NB100-60411-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CHD1 Antibody [NB100-60411] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and CHD1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:2000 of Rabbit Anti-Human CHD1 Polyclonal Antibody (Catalog # NB100-60411) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for CHD1 at approximately 260 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: CHD1 Antibody [NB100-60411] - Detection of Human CHD1 on HeLa whole cell lysate using NB100-60411. CHD1 was also immunoprecipitated by rabbit anti-CHD1 antibody NB100-60410.](http://images.novusbio.com/fullsize/CHD1-Antibody-Western-Blot-NB100-60411-img0005.jpg)
![Chromatin Immunoprecipitation Sequencing: CHD1 Antibody [NB100-60411] - Localization of CHD1 Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-CHD1 antibody NB100-60411 and analyzed by DNA sequencing. The figure illustrates the peak distribution of CHD1 binding within a 3.5 Mb region of the human X chromosome as detected using anti-CHD1 antibody NB100-60411. ChIP-seq validation performed by Diogenode, Denville, NJ.](http://images.novusbio.com/fullsize/CHD1-Antibody-Chromatin-Immunoprecipitation-Sequencing-NB100-60411-img0006.jpg)
![Chromatin Immunoprecipitation: CHD1 Antibody [NB100-60411] - ChIP-chip scatter plot of anti-CHD1 (NB100-60411) enriched DNA binding sites versus input reference DNA. CHD1 was used to immunoprecipitate chromatin from K562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-CHD1 ChIP, normal rabbit IgG showed little enrichment.](http://images.novusbio.com/fullsize/CHD1-Antibody-Chromatin-Immunoprecipitation-NB100-60411-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: CHD1 Antibody [NB100-60411] - Chromatin marks associated with transcriptional elongation are increased in the absence of Lsh. Western analysis for detection of Chd1 protein comparing whole cell extracts derived from Lsh-/-MEFs targeted by Chd1 siRNA or control siRNA. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0009163), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CHD1-Antibody-Knockdown-Validated-NB100-60411-img0008.jpg)