| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22110SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05950SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-111SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-139SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80687 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-117SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54792SS | |||
| Azide and BSA Free | NBP2-80556 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76798SS | |||
| Azide and BSA Free | NBP2-80879 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25154SS | |||
![Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of LXR alpha/NR1H3 on rat brain tissue lysates with Rabbit anti-LXR alpha antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Western-Blot-NBP2-66938-img0009.jpg "Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of LXR alpha/NR1H3 on rat brain tissue lysates with Rabbit anti-LXR alpha antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Staining LXR alpha/NR1H3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Immunocytochemistry-Immunofluorescence-NBP2-66938-img0002.jpg)
![Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of paraffin-embedded mouse testes tissue using anti-LXR alpha/NR1H3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Immunohistochemistry-Paraffin-NBP2-66938-img0005.jpg)
![Flow Cytometry: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of HepG2 cells with LXR alpha/NR1H3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Flow-Cytometry-NBP2-66938-img0001.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of LXR alpha on mouse colon tissue lysate using anti-LXR alpha/NR1H3 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Western-Blot-NBP2-66938-img0006.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of LXR alpha/NR1H3 on human liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Western-Blot-NBP2-66938-img0007.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of LXR alpha/NR1H3 on mouse colon tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Western-Blot-NBP2-66938-img0008.jpg)
![Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of paraffin-embedded human placenta tissue using anti-LXR alpha/NR1H3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Immunohistochemistry-Paraffin-NBP2-66938-img0003.jpg)
![Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of paraffin-embedded human spleen tissue using anti-LXR alpha/NR1H3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Immunohistochemistry-Paraffin-NBP2-66938-img0004.jpg)
![Western Blot: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110] - Analysis using ApoE mAb against HEK293 (1) and ApoE (AA: 20-267)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ApoE-Antibody-1H4-Western-Blot-NBP2-22110-img0002.jpg "Western Blot: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110] - Analysis using ApoE mAb against HEK293 (1) and ApoE (AA: 20-267)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110] - Analysis of paraffin-embedded liver cancer tissues (left) and brain tissues (right) using ApoE mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ApoE-Antibody-1H4-Immunohistochemistry-Paraffin-NBP2-22110-img0004.jpg)
![Flow Cytometry: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110] - Analysis of HepG2 cells using ApoE mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/ApoE-Antibody-1H4-Flow-Cytometry-NBP2-22110-img0003.jpg)
![ELISA: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110] - Red: Control Antigen (100 ng); Purple: Antigen (10 ng); Green: Antigen (50 ng); Blue: Antigen (100 ng).](http://images.novusbio.com/fullsize/ApoE-Antibody-1H4-ELISA-NBP2-22110-img0001.jpg)
![Western Blot: LCAT Antibody [NBP1-05950] - Analysis of plasma LCAT protein mass. Plasma samples (0.2ul) from four hA-I Tg mice, four hA-I Tg SR-BI -/- mice, a LCAT-/- mouse, and purified mouse LCAT were fractionated on SDS-PAGE, and LCAT was detected by Western blot analysis using rabbit antiserum against mouse LCAT.](http://images.novusbio.com/fullsize/LCAT-Antibody-Western-Blot-NBP1-05950-img0003.jpg "Western Blot: LCAT Antibody [NBP1-05950] - Analysis of plasma LCAT protein mass. Plasma samples (0.2ul) from four hA-I Tg mice, four hA-I Tg SR-BI -/- mice, a LCAT-/- mouse, and purified mouse LCAT were fractionated on SDS-PAGE, and LCAT was detected by Western blot analysis using rabbit antiserum against mouse LCAT.")
![Immunocytochemistry/Immunofluorescence: LCAT Antibody [NBP1-05950] - The LCAT antibody was tested at a 1:500 dilution in HeLa cells against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue).](http://images.novusbio.com/fullsize/LCAT-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-05950-img0004.jpg)
![Western Blot: Endothelial Lipase Antibody [NB400-111] - Human endothelial lipase detected in transfected 293 lysates (MW 57 kDa)](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-Western-Blot-NB400-111-img0007.jpg "Western Blot: Endothelial Lipase Antibody [NB400-111] - Human endothelial lipase detected in transfected 293 lysates (MW 57 kDa)")
![Immunocytochemistry/Immunofluorescence: Endothelial Lipase Antibody [NB400-111] - Antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-Immunocytochemistry-Immunofluorescence-NB400-111-img0001.jpg)
![Western Blot: Endothelial Lipase Antibody [NB400-111] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY LIPG cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIPG.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-Western-Blot-NB400-111-img0006.jpg)
![Western Blot: Apolipoprotein A5 Antibody (1G5G9) [NB400-139] - Analysis of ApoA5 in HepG2 lysates.](http://images.novusbio.com/fullsize/Apolipoprotein-A5-Antibody-1G5G9-Western-Blot-NB400-139-img0005.jpg "Western Blot: Apolipoprotein A5 Antibody (1G5G9) [NB400-139] - Analysis of ApoA5 in HepG2 lysates.")
![Immunocytochemistry/Immunofluorescence: Apolipoprotein A5 Antibody (1G5G9) [NB400-139] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Apolipoprotein A5 (1G5G9) at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Apolipoprotein-A5-Antibody-1G5G9-Immunocytochemistry-Immunofluorescence-NB400-139-img0006.jpg)
![Flow Cytometry: Apolipoprotein A5 Antibody (1G5G9) [NB400-139] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Apolipoprotein A5 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Apolipoprotein-A5-Antibody-1G5G9-Flow-Cytometry-NB400-139-img0004.jpg)
![Western Blot: Apolipoprotein A5 Antibody (1G5G9) [NB400-139] - Analysis using anti- human APOA5 monoclonal antibody against human serum total protein (lane 2). Lane 1 is a MW standard.](http://images.novusbio.com/fullsize/Apolipoprotein-A5-Antibody-1G5G9-Western-Blot-NB400-139-img0003.jpg)
![Western Blot: Apolipoprotein A5 Antibody (1G5G9) [NB400-139] - Detection of Apo A5 in HepG2 cell lysate with NB400-139.](http://images.novusbio.com/fullsize/Apolipoprotein-A5-Antibody-1G5G9-Western-Blot-NB400-139-img0002.jpg)
![Western Blot: Cytochrome c Antibody [NBP2-21569] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-Western-Blot-NBP2-21569-img0014.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Cytochrome c Antibody [NBP2-21569] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Cytochrome c Antibody [NBP2-21569] - Human endometrial carcinoma. Cytochrome C stained by Cytochrome C antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-Immunohistochemistry-Paraffin-NBP2-21569-img0008.jpg)
![Western Blot: Cytochrome C Antibody [NBP2-21569] - Sample (30 ug of whole cell lysate) A: PC-12 15% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Cytochrome-C-Antibody-Western-Blot-NBP2-21569-img0002.jpg)
![Western Blot: Cytochrome C Antibody [NBP2-21569] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 15% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Cytochrome-C-Antibody-Western-Blot-NBP2-21569-img0003.jpg)
![Western Blot: Cytochrome c Antibody [NBP2-21569] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with Cytochrome C antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident pico Western HRP Substrate.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-Western-Blot-NBP2-21569-img0011.jpg)
![Immunohistochemistry-Paraffin: Cytochrome c Antibody [NBP2-21569] - Human cervical carcinoma. Cytochrome C stained by Cytochrome C antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-Immunohistochemistry-Paraffin-NBP2-21569-img0007.jpg)
![Simple Western: Cytochrome c Antibody [NBP2-21569] - Cytochrome C Antibodies (NBP2-21569), ProteinSimple Western Blot on Jess Instrument; 1 microgram of human brain tissue lysate was tested with the antibodies diluted 10 and 20 times. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-21569_rabbit-polyclonal-cytochrome-c-antibody-simple-western-204202311476.jpg)
![Western Blot: Cytochrome c Antibody [NBP2-21569] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome c antibody (NBP2-21569) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-21569_rabbit-polyclonal-cytochrome-c-antibody-91020242063356.jpg)
![Western Blot: Cytochrome c Antibody [NBP2-21569] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome c antibody (NBP2-21569) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-21569_rabbit-polyclonal-cytochrome-c-antibody-91020242063396.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Cytochrome c Antibody [NBP2-21569] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-21569_rabbit-polyclonal-cytochrome-c-antibody-1610202419405762.jpg)
![Immunoprecipitation: Cytochrome c Antibody (6H2.B4) [NBP2-24872] - Immunoprecitation/analysis using cytochrome c antibodies. Two micrograms of cytochrome c antibody clone 6H2.B4 (lane 1) or control IgG antibody (lane 2) were used to immunoprecipitate cytochrome c from 1 x 10^6 HeLa cells. The immunoprecipitated protein was resolved by SDS-PAGE and probed with cytochrome antibody clone 7H8.2C12 .](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-6H2-B4-Immunoprecipitation-NBP2-24872-img0004.jpg "Immunoprecipitation: Cytochrome c Antibody (6H2.B4) [NBP2-24872] - Immunoprecitation/analysis using cytochrome c antibodies. Two micrograms of cytochrome c antibody clone 6H2.B4 (lane 1) or control IgG antibody (lane 2) were used to immunoprecipitate cytochrome c from 1 x 10^6 HeLa cells. The immunoprecipitated protein was resolved by SDS-PAGE and probed with cytochrome antibody clone 7H8.2C12 .")
![Immunoprecipitation: Cytochrome c Antibody (6H2.B4) [NBP2-24872] - Cytochrome c was immunoprecipitated from Hela cell extract (1% NP-40) using 2-4 ug 6H2.B4 antibody/1 x107 cell equivalents.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-6H2-B4-Immunoprecipitation-NBP2-24872-img0005.jpg)
![Western Blot: ABCG8 Antibody [NB400-117] - Total protein from Mouse small intestine was separated on a 7.5% gel by SDS-PAGE and transferred to PVDF membrane. The membrane was then probed with anti-ABCG8 antibody at 2 ug/ml and detected with an anti-rabbit HRP conjugated secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/ABCG8-Antibody-Western-Blot-NB400-117-img0003.jpg "Western Blot: ABCG8 Antibody [NB400-117] - Total protein from Mouse small intestine was separated on a 7.5% gel by SDS-PAGE and transferred to PVDF membrane. The membrane was then probed with anti-ABCG8 antibody at 2 ug/ml and detected with an anti-rabbit HRP conjugated secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: ABCG8 Antibody [NB400-117] - ABCG8 antibody was tested in HEK293 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/ABCG8-Antibody-Immunocytochemistry-Immunofluorescence-NB400-117-img0002.jpg)
![Western Blot: ABCA1 Antibody (1276B) [NBP2-54792] - Mouse Raw264.7 and human THP-1 cells were treated with and without the LXR agonist TO901317 (1 uM) for 24 hours. Total protein was then separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-ABCA1 in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/ABCA1-Antibody-1276B-Western-Blot-NBP2-54792-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: ABCA1 Antibody (1276B) [NBP2-54792] - Mouse Raw264.7 and human THP-1 cells were treated with and without the LXR agonist TO901317 (1 uM) for 24 hours. Total protein was then separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-ABCA1 in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: ABCA1 Antibody (1276B) [NBP2-54792] - Analysis of a FFPE tissue section of mouse liver using ABCA1 antibody (1276B) Lot ckjv02 at 1:100 dilution. The primary antibody bound to ABCA1 antigen in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This ABCA1 antibody generated specific staining in the membranes of the hepatocytes and the Kupffer cells. As expected for ABCA1, the staining signal was stronger in the Kupffer cells which are the tissue macrophages in liver.](http://images.novusbio.com/fullsize/ABCA1-Antibody-1276B-Immunohistochemistry-Paraffin-NBP2-54792-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: ABCA1 Antibody (1276B) [NBP2-54792] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-ABCA1 at 10 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ABCA1-Antibody-1276B-Immunocytochemistry-Immunofluorescence-NBP2-54792-img0003.jpg)
![Flow (Intracellular): ABCA1 Antibody (1276B) [NBP2-54792] - An intracellular stain was performed on HepG2 Cells with ABCA1 (1276B) antibody NBP2-54792 (blue) and a matched isotype control MAB1050 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/ABCA1-Antibody-1276B-Flow-Intracellular-NBP2-54792-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ABCA1 Antibody (1276B) [NBP2-54792] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-ABCA1 at 10 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ABCA1-Antibody-1276B-Immunocytochemistry-Immunofluorescence-NBP2-54792-img0002.jpg)
![Immunohistochemistry-Paraffin: ABCA1 Antibody (1276B) [NBP2-54792] - Analysis of a FFPE tissue section of mouse liver using ABCA1 antibody (1276B) Lot ckjv0116091 at 1:50 dilution. The primary antibody bound to ABCA1 antigen in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This ABCA1 antibody generated specific staining in the membranes of the hepatocytes and the Kupffer cells. As expected for ABCA1, the staining signal was stronger in the Kupffer cells which are the tissue macrophages in liver.](http://images.novusbio.com/fullsize/ABCA1-Antibody-1276B-Immunohistochemistry-Paraffin-NBP2-54792-img0005.jpg)
![Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Niemann-Pick Type C1/NPC1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (Catalog # MAB10105) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Niemann-Pick Type C1/NPC1 at approximately 170-250 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Knockout-Validated-NBP2-76798-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Niemann-Pick Type C1/NPC1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (Catalog # MAB10105) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Niemann-Pick Type C1/NPC1 at approximately 170-250 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 for 5 minutes. The cells were incubated with anti- Niemann-Pick C1 Antibody at 2 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunocytochemistry-Immunofluorescence-NBP2-76798-img0006.jpg)
![Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Total protein from human HeLa, Hek293, mouse 3T3 and Rat PC12 cell lines were separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NPC1 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Western-Blot-NBP2-76798-img0005.jpg)
![Immunohistochemistry-Paraffin: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Niemann-Pick Type C1/NPC1 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (NBP2-76798) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic puncta.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunohistochemistry-Paraffin-NBP2-76798-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Niemann-Pick C1 Antibody (1318A) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunocytochemistry-Immunofluorescence-NBP2-76798-img0004.jpg)
![Western Blot: FABP2/I-FABP Antibody [NB100-59746] - (0.001ug/ml) staining of Human Duodenum (A) and (0.003ug/ml) Ileum (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence](http://images.novusbio.com/fullsize/FABP2-I-FABP-Antibody-Western-Blot-NB100-59746-img0012.jpg "Western Blot: FABP2/I-FABP Antibody [NB100-59746] - (0.001ug/ml) staining of Human Duodenum (A) and (0.003ug/ml) Ileum (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence")
![Immunohistochemistry-Paraffin: FABP2/I-FABP Antibody [NB100-59746] - (2.5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/FABP2-I-FABP-Antibody-Immunohistochemistry-Paraffin-NB100-59746-img0010.jpg)
![Flow Cytometry: FABP2/I-FABP Antibody [NB100-59746] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/FABP2-I-FABP-Antibody-Flow-Cytometry-NB100-59746-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5H6) [NBP2-25154] - Analysis of HeLa cells stained with mouse LAMP-1/CD107a mAb, dilution 1:500 (Red), and costained with chicken Vimentin pAb, dilution 1:10000, (Green). DAPI staining of nuclear DNA (Blue). The cells were treated with 50uM of chloroquine, an inhibitor of autophagy, for 16hrs prior to staining. The LAMP-1/CD107a antibody reveals vesicular staining of LAMP1 protein accumulated in swollen lysosomes, while the Vimentin antibody specifically labels the intermediate filament network in these cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-5H6-Immunocytochemistry-Immunofluorescence-NBP2-25154-img0007.jpg "Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5H6) [NBP2-25154] - Analysis of HeLa cells stained with mouse LAMP-1/CD107a mAb, dilution 1:500 (Red), and costained with chicken Vimentin pAb, dilution 1:10000, (Green). DAPI staining of nuclear DNA (Blue). The cells were treated with 50uM of chloroquine, an inhibitor of autophagy, for 16hrs prior to staining. The LAMP-1/CD107a antibody reveals vesicular staining of LAMP1 protein accumulated in swollen lysosomes, while the Vimentin antibody specifically labels the intermediate filament network in these cells.")
![Western Blot: LAMP-1/CD107a Antibody (5H6) [NBP2-25154] - Analysis of different cell lysates using mouse mAb to LAMP1, NBP2-25154 dilution 1:10,000 in green. Cells were maintained under normal conditions (Ct), or treated with 50uM of chloroquine (CQ), an inhibitor of autophagy, for 24 hours: [1] protein standard (red), [2] HeLa Ct, [3] HeLa+CQ, [4] NIH-3T3 Ct, and [5] NIH-3T3+CQ. The smeared band between 75-120kDa corresponds to variably glycosylated forms of the LAMP1 protein detected only in the human cells, this antibody fails to recognize the rodent homologue. The same blot was probed with chicken pAB to HSP60, dilution 1:20,000. The HSP60 antibody reveals heat shock 60 protein with apparent molecular weight of 60kDa in all preparations.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-5H6-Western-Blot-NBP2-25154-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5H6) [NBP2-25154] - D2.0 R cells activate autophagy upon entering a dormant state. Representative images are shown for immunofluorescent staining of lysosomal (LAMP-1/CD107a, red) and autophagic (LC3, green) markers of D2.0 R cells in BME (upper panels) and BME + COL1 (lower panels). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-04070-6), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-5H6-Immunocytochemistry-Immunofluorescence-NBP2-25154-img0008.jpg)