| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44977 | |||
| Azide and BSA Free | NBP2-34817-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB18771-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50040SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44980 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21157-25ul | |||
| Azide and BSA Free | NBP3-44279 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85173-25ul | |||
![Western Blot: p53 Antibody (SPM589) [NBP2-32891] - Western Blot Analysis of HeLa cell lysate using p53 Antibody (SPM589)](http://images.novusbio.com/fullsize/p53-Antibody-SPM589-Western-Blot-NBP2-32891-img0004.jpg "Western Blot: p53 Antibody (SPM589) [NBP2-32891] - Western Blot Analysis of HeLa cell lysate using p53 Antibody (SPM589)")
![Immunohistochemistry-Paraffin: p53 Antibody (SPM589) [NBP2-32891] - Formalin-fixed paraffin-embedded human bladder carcinoma stained with p53 monoclonal antibody (SPM589)](http://images.novusbio.com/fullsize/p53-Antibody-SPM589-Immunohistochemistry-Paraffin-NBP2-32891-img0003.jpg)
![Western Blot: HMGN1/HMG14 [p Ser20, p Ser24] Antibody [NBP1-78102] - HMGN1, non-phosphorylated, 2 - HMGN1, phosphorylated, 3 - HMGN1 delta20E, delta24E, non-phosphorylated, 4 - HMGN1, delta20E, delta24E, phosphorylated, 5 - HMGN2, non-phosphorylated, and 6 - HMGN2, phosphorylated. Molecular weight markers (not shown) confirm the size of each recombinant protein. The primary antibody was diluted 1:1,000 for this experiment. The blot was processed using a 5 sec exposure time.](http://images.novusbio.com/fullsize/HMGN1-HMG14-[p-Ser20--p-Ser24]-Antibody-Western-Blot-NBP1-78102-img0001.jpg "Western Blot: HMGN1/HMG14 [p Ser20, p Ser24] Antibody [NBP1-78102] - HMGN1, non-phosphorylated, 2 - HMGN1, phosphorylated, 3 - HMGN1 delta20E, delta24E, non-phosphorylated, 4 - HMGN1, delta20E, delta24E, phosphorylated, 5 - HMGN2, non-phosphorylated, and 6 - HMGN2, phosphorylated. Molecular weight markers (not shown) confirm the size of each recombinant protein. The primary antibody was diluted 1:1,000 for this experiment. The blot was processed using a 5 sec exposure time.")
![Immunohistochemistry: HMGN1/HMG14 [p Ser20, p Ser24] Antibody [NBP1-78102] - Used at 20 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate nuclear and faint cytoplasmic positive staining of epidermal keratinocytes at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/HMGN1-HMG14-[p-Ser20-p-Ser24]-Antibody-Immunohistochemistry-NBP1-78102-img0003.jpg)
![Western Blot: 53BP1 Antibody (1285A) [MAB18771] - Analysis of HEK293 and ZR-75 cell lysates with 1 ug/ml of h53BP1 Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. The molecular weight was observed for h53BP1 at approximately 350 kDa (as indicated), and the theoretical molecular weight of the whole endogenous protein is 214 kDa. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.](http://images.novusbio.com/fullsize/53BP1-Antibody-1285A-Western-Blot-MAB18771-img0002.jpg "Western Blot: 53BP1 Antibody (1285A) [MAB18771] - Analysis of HEK293 and ZR-75 cell lysates with 1 ug/ml of h53BP1 Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. The molecular weight was observed for h53BP1 at approximately 350 kDa (as indicated), and the theoretical molecular weight of the whole endogenous protein is 214 kDa. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.")
![Immunocytochemistry: 53BP1 Antibody (1285A) [MAB18771] - 53BP1 was detected in immersion fixed HeLa cell using 53BP1 Monoclonal Antibody at 3 ug/ml dilution. Cells were stained using the NorthernLights 557-conjugated Anti-Rabbit IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to nuclei.](http://images.novusbio.com/fullsize/53BP1-Antibody-1285A-Immunocytochemistry-MAB18771-img0001.jpg)
![Western Blot: Aurora A Antibody (1A11) [NBP2-50040] - Analysis of different cell lysates and recombinant protein solutions using mouse Aurora A mAb. Left: cells were treated with 100ng/mL of nocodazol (a microtubule depolymerizing agent which induces cells to halt at G2/M phase) for 6hrs: [1] protein standard, [2] HeLa, [3] canine A72 cells, [4] Equine NBL6 cells, and [5] murine KR158 cells. The antibody apparently does not recognize canine or equine aurora A, but does bind human and murine. Right: equal amounts of purified human recombinant aurora A, B, C as indicated. The NBP2-50040 antibody reacts with the aurora A recombinant protein but not aurora B or C.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1A11-Western-Blot-NBP2-50040-img0005.jpg "Western Blot: Aurora A Antibody (1A11) [NBP2-50040] - Analysis of different cell lysates and recombinant protein solutions using mouse Aurora A mAb. Left: cells were treated with 100ng/mL of nocodazol (a microtubule depolymerizing agent which induces cells to halt at G2/M phase) for 6hrs: [1] protein standard, [2] HeLa, [3] canine A72 cells, [4] Equine NBL6 cells, and [5] murine KR158 cells. The antibody apparently does not recognize canine or equine aurora A, but does bind human and murine. Right: equal amounts of purified human recombinant aurora A, B, C as indicated. The NBP2-50040 antibody reacts with the aurora A recombinant protein but not aurora B or C.")
![Immunocytochemistry/Immunofluorescence: Aurora A Antibody (1A11) [NBP2-50040] - HeLa cell cultures stained with NBP2-50040 (green). Aurora A localizes in spindle poles and mitotic spindles at late mitosis. Counterstained is chicken polyclonal antibody against Vimentin (NB300-223, red). Blue is a DNA stain.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1A11-Immunocytochemistry-Immunofluorescence-NBP2-50040-img0003.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (BP53-12 + DO-7) - Azide and BSA Free [NBP2-34563] - Formalin-fixed, paraffin-embedded human cervical carcinoma stained with P53 Monoclonal antibody (BP53-12 +DO-7)](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-+-DO-7-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34563-img0001.jpg "Immunohistochemistry-Paraffin: p53 Antibody (BP53-12 + DO-7) - Azide and BSA Free [NBP2-34563] - Formalin-fixed, paraffin-embedded human cervical carcinoma stained with P53 Monoclonal antibody (BP53-12 +DO-7)")
![Immunohistochemistry-Paraffin: PERP Antibody [NBP1-85173] - Staining of human esophagus shows very weak membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/PERP-Antibody-Immunohistochemistry-Paraffin-NBP1-85173-img0010.jpg "Immunohistochemistry-Paraffin: PERP Antibody [NBP1-85173] - Staining of human esophagus shows very weak membranous positivity in squamous epithelial cells.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PERP Antibody [NBP1-85173] - Analysis in human skin and skeletal muscle tissues. Corresponding PERP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PERP-Antibody-Immunohistochemistry-Paraffin-NBP1-85173-img0006.jpg)
![Immunohistochemistry-Paraffin: PERP Antibody [NBP1-85173] - Staining of human skin shows moderate to strong membranous positivity in stratum corneum.](http://images.novusbio.com/fullsize/PERP-Antibody-Immunohistochemistry-Paraffin-NBP1-85173-img0007.jpg)
![Immunohistochemistry-Paraffin: PERP Antibody [NBP1-85173] - Staining of human lymph node shows no positivity as expected.](http://images.novusbio.com/fullsize/PERP-Antibody-Immunohistochemistry-Paraffin-NBP1-85173-img0008.jpg)
![Immunohistochemistry-Paraffin: PERP Antibody [NBP1-85173] - Staining of human endometrium shows no positivity as expected.](http://images.novusbio.com/fullsize/PERP-Antibody-Immunohistochemistry-Paraffin-NBP1-85173-img0009.jpg)
![Immunohistochemistry-Paraffin: PERP Antibody [NBP1-85173] - Staining of human skeletal muscle shows no positivity as expected.](http://images.novusbio.com/fullsize/PERP-Antibody-Immunohistochemistry-Paraffin-NBP1-85173-img0011.jpg)
![Western Blot: Chk1 Antibody [NB100-311] - Samples: Whole cell lysate (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Chk1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-311-img0200.jpg "<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-311] - Samples: Whole cell lysate (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Chk1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-311] - Whole Cell lysate (50 ug for WB, 500 ug for IP) from HeLa cells. Affinity purified goat anti-Chk1 used as indicated. Immunoprecipitated material was detected by WB using a monoclonal antibody to Chk1. Detection: Chemiluminescence with 1 minute exposure.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-311-img0003.jpg)
![Western Blot: ATM Antibody [NB100-271] - Detection of human ATM by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat ATM antibody [NB100-271] used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~360 kDa. Theoretical molecular weight 351 kDa.](http://images.novusbio.com/fullsize/ATM-Antibody-Western-Blot-NB100-271-img0004.jpg "Western Blot: ATM Antibody [NB100-271] - Detection of human ATM by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat ATM antibody [NB100-271] used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~360 kDa. Theoretical molecular weight 351 kDa.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: ATM Antibody [NB100-271] - Detection of human ATM by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat ATM antibody [NB100-271] used for IP at 3 ug per reaction. ATM was also immunoprecipitated by another goat anti-ATM antibody from Company B. For blotting immunoprecipitated ATM, ATM antibody [NB100-271] was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ATM-Antibody-Immunoprecipitation-NB100-271-img0005.jpg)
![Western Blot: CDC25A Antibody [NB100-213] - Detection of human CDC25a by western blot. Samples: Nuclear extract (50 µg) from HeLa and HEK293T cells. Antibody: Affinity purified rabbit anti-CDC25a antibody NB100-213 used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CDC25A-Antibody-Western-Blot-NB100-213-img0009.jpg "Western Blot: CDC25A Antibody [NB100-213] - Detection of human CDC25a by western blot. Samples: Nuclear extract (50 µg) from HeLa and HEK293T cells. Antibody: Affinity purified rabbit anti-CDC25a antibody NB100-213 used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Western Blot: CDC25A Antibody [NB100-213] - Samples: Nuclear Extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-CDC25a antibody (lot was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CDC25A-Antibody-Western-Blot-NB100-213-img0006.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: CDC25A Antibody [NB100-213] - Detection of Human CDC25a by Western Blot. Samples: Nuclear extract (50 ug) from HeLa and 293T cells. Antibody: Affinity purified rabbit anti-CDC25a antibody NB100-213 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CDC25A-Antibody-Western-Blot-NB100-213-img0008.jpg)
![Western Blot: Rabbit Polyclonal CDC25A Antibody [NB100-213] - Whole cell lysates from MDA-MB-231, SUM159, MCF-7 and HEK293T cells were loaded with 50 ug/lane. 10% SDS-PAGE. CDC25A Antibody (NB100-213) was used for primary antibody: 1:1000, 4℃, overnight. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb100-213_rabbit-polyclonal-cdc25a-antibody-western-blot-3112024151137..png)
![Western Blot: RPA2 [p Ser8, p Ser4] Antibody (BL-165-5F1) [NBP2-76396] - Detection of human Phospho RPA32 (S4/S8) by WB of HeLa lysate treated with 100 uM EPE (+) or mock treated (-). Antibodies: Rabbit anti-Phospho RPA32 (S4/S8) recombinant monoclonal [BL-165-5F1] (NBP2-76396). Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/RPA2-p-Ser8-p-Ser4-Antibody-BL-165-5F1-Western-Blot-NBP2-76396-img0001.jpg "Western Blot: RPA2 [p Ser8, p Ser4] Antibody (BL-165-5F1) [NBP2-76396] - Detection of human Phospho RPA32 (S4/S8) by WB of HeLa lysate treated with 100 uM EPE (+) or mock treated (-). Antibodies: Rabbit anti-Phospho RPA32 (S4/S8) recombinant monoclonal [BL-165-5F1] (NBP2-76396). Secondary: HRP-conjugated goat anti-rabbit IgG.")
![Immunocytochemistry/Immunofluorescence: RPA2 [p Ser8, p Ser4] Antibody (BL-165-5F1) [NBP2-76396] - Rabbit anti-Phospho RPA32 (S4/S8) recombinant monoclonal antibody [BL-165-5F1] .Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/RPA2-p-Ser8-p-Ser4-Antibody-BL-165-5F1-Immunocytochemistry-Immunofluorescence-NBP2-76396-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: RPA2 [p Ser8, p Ser4] Antibody (BL-165-5F1) [NBP2-76396] - Detection of Human Phospho RPA32 (S4/S8) in NBF-fixed HeLa cells treated with EPE (right) or mock treated (left) by ICC-IF. Antibody: Rabbit anti-Phospho RPA32 (S4/S8) recombinant monoclonal [BL-165-5F1] (NBP2-76396). Detection: DyLight® 594-conjugated goat anti-rabbit IgG. Counterstain: DAPI (blue).](http://images.novusbio.com/fullsize/RPA2-p-Ser8-p-Ser4-Antibody-BL-165-5F1-Immunocytochemistry-Immunofluorescence-NBP2-76396-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: RPA2 [p Ser8, p Ser4] Antibody (BL-165-5F1) [NBP2-76396] - Detection of human Phospho RPA32 (S4/S8) by WB of immunoprecipitates from HeLa lysate treated with 100 uM EPE (+) or mock treated (-). Antibodies: Rabbit anti-Phospho RPA32 (S4/S8) recombinant monoclonal [BL-165-5F1] (NBP2-76396) and another rabbit anti-RPA32 antibody.](http://images.novusbio.com/fullsize/RPA2-p-Ser8-p-Ser4-Antibody-BL-165-5F1-Immunoprecipitation-NBP2-76396-img0003.jpg)
![Immunoprecipitation: ATR [p Thr1989] Antibody (222) [NBP2-43564] - Analysis of ATR protein from HCT-116 whole cell extracts using 5 ug of ATR antibody or ATR antibody. Western blot analysis was performed using ATR antibody. EasyBlot anti-Mouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/ATR-p-Thr1989-Antibody-222-Immunoprecipitation-NBP2-43564-img0001.jpg "<b>Biological Strategies Validation. </b>Immunoprecipitation: ATR [p Thr1989] Antibody (222) [NBP2-43564] - Analysis of ATR protein from HCT-116 whole cell extracts using 5 ug of ATR antibody or ATR antibody. Western blot analysis was performed using ATR antibody. EasyBlot anti-Mouse IgG was used as a secondary reagent.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: ATR [p Thr1989] Antibody (222) [NBP2-43564] - ATR antibody [GT222] detects ATR protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ATR stained by ATR antibody [GT222] (NBP2-43564) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-43564_mouse-monoclonal-atr-p-thr1989-antibody-222-910202419593539.jpg)
![Western Blot: ATR [p Thr1989] Antibody (222) [NBP2-43564] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody [GT222] (NBP2-43564) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43564_mouse-monoclonal-atr-p-thr1989-antibody-222-91020242014673.jpg)
![Immunocytochemistry/ Immunofluorescence: ATR [p Thr1989] Antibody (222) [NBP2-43564] - ATR antibody [GT222] detects ATR protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ATR stained by ATR antibody [GT222] (NBP2-43564) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-43564_mouse-monoclonal-atr-p-thr1989-antibody-222-91020242075915.jpg)
![Untreated (-) and treated (+) HCT116 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody [GT222] (NBP2-43564) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-43564_mouse-monoclonal-atr-p-thr1989-antibody-222-western-blot-261120251983622.jpg)