| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76781-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-51596-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-05379-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48410 | |||
| Azide and BSA Free | NBP3-11546 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59628 | |||
![Western Blot: ATM [p Ser1981] Antibody (HL1062) [NBP3-13705] - Untreated (-) and treated (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM (phospho Ser1981) antibody [HL1062] (NBP3-13705) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ATM-p-Ser1981-Antibody-HL1062-Western-Blot-NBP3-13705-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: ATM [p Ser1981] Antibody (HL1062) [NBP3-13705] - Untreated (-) and treated (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM (phospho Ser1981) antibody [HL1062] (NBP3-13705) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ATM [p Ser1981] Antibody (HL1062) [NBP3-13705] - ATM (phospho Ser1981) antibody [HL1062] detects ATM (phospho Ser1981) protein at nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATM (phospho Ser1981) stained by ATM (phospho Ser1981) antibody [HL1062] (NBP3-13705) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/ATM-p-Ser1981-Antibody-HL1062-Immunocytochemistry-Immunofluorescence-NBP3-13705-img0003.jpg)
![Immunohistochemistry-Paraffin: ATM [p Ser1981] Antibody (HL1062) [NBP3-13705] - ATM (phospho Ser1981) antibody [HL1062] detects ATM (phospho Ser1981) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. ATM (phospho Ser1981) stained by ATM (phospho Ser1981) antibody [HL1062] (NBP3-13705) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/ATM-p-Ser1981-Antibody-HL1062-Immunohistochemistry-Paraffin-NBP3-13705-img0002.jpg)
![Untreated (-) and treated (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with ATM (phospho Ser1981) antibody [HL1062] (NBP3-13705) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13705_rabbit-atm-p-ser-1981-mab-hl1062-western-blot-261120251845135.jpg)
![Untreated (-) and treated (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM (phospho Ser1981) antibody [HL1062] (NBP3-13705) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13705_rabbit-atm-p-ser-1981-mab-hl1062-western-blot-261120251836813.jpg)
![Western Blot: Nbs1 Antibody [NB100-60654] - Detection of human Nbs1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Nbs1 was also immunoprecipitated by rabbit anti-Nbs1 antibodies NB100-60653 and NB100-60654, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/Nbs1-Antibody-Western-Blot-NB100-60654-img0017.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Nbs1 Antibody [NB100-60654] - Detection of human Nbs1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Nbs1 was also immunoprecipitated by rabbit anti-Nbs1 antibodies NB100-60653 and NB100-60654, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: Nbs1 Antibody [NB100-60654] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used at a dilution of 1:200 (1ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/Nbs1-Antibody-Immunohistochemistry-NB100-60654-img0027.jpg)
![Western Blot: Rad17 [p Ser645] Antibody [NB100-273] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells treated with 100 uM EPE for 2 hours (+) or mock treated (-) cells. Antibodies : Affinity purified rabbit anti-Rad17, Phospho (S645) antibody . Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower panels).](http://images.novusbio.com/fullsize/Rad17-[p-Ser645]-Antibody-Western-Blot-NB100-273-img0045.jpg "<b>Biological Strategies Validation. </b>Western Blot: Rad17 [p Ser645] Antibody [NB100-273] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells treated with 100 uM EPE for 2 hours (+) or mock treated (-) cells. Antibodies : Affinity purified rabbit anti-Rad17, Phospho (S645) antibody . Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower panels).")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: Rad17 [p Ser645] Antibody [NB100-273] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-Phospho Rad17 (S645) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Rad17-[p-Ser645]-Antibody-Immunohistochemistry-NB100-273-img0049.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Rad17 [p Ser645] Antibody [NB100-273] - Whole cell lysate (50 ug) from HeLa cells before (-) or after (+) treatment with 10 Gy ionizing radiation. Antibody used at 1 ug/ml.](http://images.novusbio.com/fullsize/Rad17-[p-Ser645]-Antibody-Western-Blot-NB100-273-img0002.jpg)
![Immunohistochemistry: Rad17 [p Ser645] Antibody [NB100-273] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Phospho Rad17 (S645) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Rad17-[p-Ser645]-Antibody-Immunohistochemistry-NB100-273-img0048.jpg)
![Western Blot: p53R2 Antibody [NBP1-76781] - Analysis of p53R2 in 3T3 cell lysate with p53R2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/p53R2-Antibody-Western-Blot-NBP1-76781-img0004.jpg "Western Blot: p53R2 Antibody [NBP1-76781] - Analysis of p53R2 in 3T3 cell lysate with p53R2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.")
![Immunocytochemistry/Immunofluorescence: p53R2 Antibody [NBP1-76781] - Immunofluorescence of p53R2 in Human Lung tissue with p53R2 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/p53R2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76781-img0003.jpg)
![Immunohistochemistry-Paraffin: p53R2 Antibody [NBP1-76781] - Human lung tissue with p53R2 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/p53R2-Antibody-Immunohistochemistry-Paraffin-NBP1-76781-img0002.jpg)
![Western Blot: 53BP1 Antibody [NBP3-14667] - Whole cell lysate (15 ug) from HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), and Jurkat cells prepared using NETN lysis buffer. Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] NBP3-14667 lot 1) used at 1:1000. HRP-conjugated goat anti-rabbit IgG (NB7156). Chemiluminescence with an exposure time of 10 second](http://images.novusbio.com/fullsize/53BP1-Antibody-Western-Blot-NBP3-14667-img0001.jpg "Western Blot: 53BP1 Antibody [NBP3-14667] - Whole cell lysate (15 ug) from HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), and Jurkat cells prepared using NETN lysis buffer. Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] NBP3-14667 lot 1) used at 1:1000. HRP-conjugated goat anti-rabbit IgG (NB7156). Chemiluminescence with an exposure time of 10 second")
![Immunohistochemistry: 53BP1 Antibody [NBP3-14667] - Sample: FFPE section of mouse renal cell carcinoma.Antibody: Rabbit anti-53BP1 recombinant monoclonalantibody used at 1:250.Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/53BP1-Antibody-Immunohistochemistry-NBP3-14667-img0004.jpg)
![Immunohistochemistry: 53BP1 Antibody [NBP3-14667] - Sample: FFPE section of colon carcinoma. Antibody: Rabbitanti-53BP1 recombinant monoclonal antibody used at 1:250. Secondary:HRP-conjugated goat anti-rabbit IgG.Substrate: DAB.](http://images.novusbio.com/fullsize/53BP1-Antibody-Immunohistochemistry-NBP3-14667-img0003.jpg)
![Immunoprecipitation: 53BP1 Antibody [NBP3-14667] - Samples: Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from HEK293T cellsprepared using NETN lysis buffer. Antibodies: Rabbitanti-53BP1 recombinant monoclonal antibodyused for IP at 20 ul perreaction. 53BP1 was also immunoprecipitated by rabbitanti-53BP1 antibody for blottingimmunoprecipitated 53BP1, was used at 1:1000.Chemiluminescence with an exposure time of 10 second.](http://images.novusbio.com/fullsize/53BP1-Antibody-Immunoprecipitation-NBP3-14667-img0002.jpg)
![Western Blot: SMC1 Antibody (BL-205-2G8) [NBP2-76412] - Detection of human and mouse SMC1 by WB of HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), Jurkat, TCMK-1, NIH 3T3, and CT26 lysate. Antibody: Rabbit anti-SMC1 recombinant monoclonal [BL-205-2G8] (NBP2-76412). Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/SMC1-Antibody-BL-205-2G8-Western-Blot-NBP2-76412-img0001.jpg "Western Blot: SMC1 Antibody (BL-205-2G8) [NBP2-76412] - Detection of human and mouse SMC1 by WB of HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), Jurkat, TCMK-1, NIH 3T3, and CT26 lysate. Antibody: Rabbit anti-SMC1 recombinant monoclonal [BL-205-2G8] (NBP2-76412). Secondary: HRP-conjugated goat anti-rabbit IgG.")
![Immunocytochemistry: SMC1 Antibody (BL-205-2G8) [NBP2-76412] - Detection of human SMC1 in FFPE HCT 116 cells by ICC. Antibody: Rabbit anti-SMC1 recombinant monoclonal [BL-205-2G8] (NBP2-76412). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/SMC1-Antibody-BL-205-2G8-Immunocytochemistry-NBP2-76412-img0004.jpg)
![Immunohistochemistry: SMC1 Antibody (BL-205-2G8) [NBP2-76412] - Detection of human SMC1 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-SMC1 recombinant monoclonal [BL-205-2G8] (NBP2-76412). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/SMC1-Antibody-BL-205-2G8-Immunohistochemistry-NBP2-76412-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: SMC1 Antibody (BL-205-2G8) [NBP2-76412] - Detection of human SMC1 by WB of immunoprecipitates from HEK293T lysate. Antibodies: Rabbit anti-SMC1 recombinant monoclonal {BL-205-2G8] (NBP2-76412) and rabbit anti-SMC1 (NB100-204).](http://images.novusbio.com/fullsize/SMC1-Antibody-BL-205-2G8-Immunoprecipitation-NBP2-76412-img0005.jpg)
![Western Blot: SMC1 Antibody (5B6) [NBP1-51596] - Analysis using SMC1 mouse mAb against K562 (1), Jurkat (2) and A549 (3) cell lysate.](http://images.novusbio.com/fullsize/SMC1-Antibody-5B6-Western-Blot-NBP1-51596-img0007.jpg "Western Blot: SMC1 Antibody (5B6) [NBP1-51596] - Analysis using SMC1 mouse mAb against K562 (1), Jurkat (2) and A549 (3) cell lysate.")
![Immunocytochemistry/Immunofluorescence: SMC1 Antibody (5B6) [NBP1-51596] - Analysis of NIH3T3 cells using SMC1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/SMC1-Antibody-5B6-Immunocytochemistry-Immunofluorescence-NBP1-51596-img0010.jpg)
![Immunohistochemistry-Paraffin: SMC1 Antibody (5B6) [NBP1-51596] - Analysis of paraffin-embedded human colon using SMC1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/SMC1-Antibody-5B6-Immunohistochemistry-Paraffin-NBP1-51596-img0008.jpg)
![Flow Cytometry: SMC1 Antibody (5B6) [NBP1-51596] - Analysis of Hela cells using SMC1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/SMC1-Antibody-5B6-Flow-Cytometry-NBP1-51596-img0009.jpg)
![Western Blot: PERP Antibody (1O3L5) [NBP3-16617] - Western blot analysis of extracts of various cell lines, using PERP Rabbit mAb (NBP3-16617) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PERP-Antibody-1O3L5-Western-Blot-NBP3-16617-img0006.jpg "Western Blot: PERP Antibody (1O3L5) [NBP3-16617] - Western blot analysis of extracts of various cell lines, using PERP Rabbit mAb (NBP3-16617) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: PERP Antibody (1O3L5) [NBP3-16617] - Immunohistochemistry of paraffin-embedded rat skin using PERP Rabbit mAb (NBP3-16617) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PERP-Antibody-1O3L5-Immunohistochemistry-Paraffin-NBP3-16617-img0001.jpg)
![Immunohistochemistry-Paraffin: PERP Antibody (1O3L5) [NBP3-16617] - Immunohistochemistry of paraffin-embedded mouse skin using PERP Rabbit mAb (NBP3-16617) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PERP-Antibody-1O3L5-Immunohistochemistry-Paraffin-NBP3-16617-img0002.jpg)
![Immunohistochemistry: PERP Antibody (1O3L5) [NBP3-16617] - Immunofluorescence analysis of human skin cells using PERP Rabbit mAb (NBP3-16617) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PERP-Antibody-1O3L5-Immunohistochemistry-NBP3-16617-img0003.jpg)
![Western Blot: Chk1 [p Ser280] Antibody [NBP3-05379] - Western blot analysis of extracts of HT-29 cells, using Phospho-Chk1-Ser280 antibody (NBP3-05379) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/Chk1-[p-Ser280]-Antibody-Western-Blot-NBP3-05379-img0005.jpg "Western Blot: Chk1 [p Ser280] Antibody [NBP3-05379] - Western blot analysis of extracts of HT-29 cells, using Phospho-Chk1-Ser280 antibody (NBP3-05379) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.")
![Immunohistochemistry-Paraffin: Chk1 [p Ser280] Antibody [NBP3-05379] - Immunohistochemistry of paraffin-embedded mouse brain using Chk1 antibody (NBP3-05379) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Chk1-[p-Ser280]-Antibody-Immunohistochemistry-Paraffin-NBP3-05379-img0003.jpg)
![Immunohistochemistry-Paraffin: Chk1 [p Ser280] Antibody [NBP3-05379] - Immunohistochemistry of paraffin-embedded rat heart using Chk1 antibody (NBP3-05379) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Chk1-[p-Ser280]-Antibody-Immunohistochemistry-Paraffin-NBP3-05379-img0001.jpg)
![Immunohistochemistry-Paraffin: Chk1 [p Ser280] Antibody [NBP3-05379] - Immunohistochemistry of paraffin-embedded human placenta using Chk1 antibody (NBP3-05379) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Chk1-[p-Ser280]-Antibody-Immunohistochemistry-Paraffin-NBP3-05379-img0002.jpg)
![Western Blot: Chk1 [p Ser280] Antibody - Azide and BSA Free [Chk1] - Western blot analysis of lysates from 293T cells, using Chk1 Rabbit pAb at 1:600 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-05379_rabbit-polyclonal-chk1-p-ser280-antibody-52202516293974.jpg)
![Western Blot: p53 Antibody (TRP/817) [NBP2-44982] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-44982) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/p53-Antibody-TRP-817-Knockout-Validated-NBP2-44982-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (TRP/817) [NBP2-44982] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-44982) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (TRP/817) [NBP2-44982] - Western Blot Analysis of HeLa cell lysate using p53 Antibody (TRP/817).](http://images.novusbio.com/fullsize/p53-Antibody-TRP-817-Western-Blot-NBP2-44982-img0005.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (TRP/817) [NBP2-44982] - Human Colon Carcinoma stained with p53 Monoclonal Antibody (TRP/817)](http://images.novusbio.com/fullsize/p53-Antibody-TRP-817-Immunohistochemistry-Paraffin-NBP2-44982-img0001.jpg)
![Western Blot: Rad17 Antibody [NBP2-27106] - Western blot analysis of Rad17 in HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) NIH 3T3 cell lysate using NBP2-27106 at 3 ug/ml.](http://images.novusbio.com/fullsize/Rad17-Antibody-Western-Blot-NBP2-27106-img0002.jpg "Western Blot: Rad17 Antibody [NBP2-27106] - Western blot analysis of Rad17 in HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) NIH 3T3 cell lysate using NBP2-27106 at 3 ug/ml.")
![Western Blot: p53 Antibody (TRP/816) [NBP2-59626] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-59626) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Knockout-Validated-NBP2-59626-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (TRP/816) [NBP2-59626] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-59626) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Protein Array: p53 Antibody (TRP/816) [NBP2-59626] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Protein-Array-NBP2-59626-img0001.jpg)
![Western Blot: p53 Antibody (TRP/816) [NBP2-59626] - Western Blot Analysis of human HeLa cell lysate using p53 Antibody (TRP/816).](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Western-Blot-NBP2-59626-img0006.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (TRP/816) [NBP2-59626] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with p53 Mouse Monoclonal Antibody (TRP/816).](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Immunohistochemistry-Paraffin-NBP2-59626-img0002.jpg)