| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48055 | |||
| Azide and BSA Free | NBP3-11516 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05771SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38622-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87772-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84584-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18057SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76772-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71097 | |||
![Western Blot: Cytokeratin 18 Antibody (DE-K18) [NBP2-44947] - Western Blot Analysis of human HCT116 cell lysate using Cytokeratin 18 Antibody (DE-K18).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-DE-K18-Western-Blot-NBP2-44947-img0008.jpg "Western Blot: Cytokeratin 18 Antibody (DE-K18) [NBP2-44947] - Western Blot Analysis of human HCT116 cell lysate using Cytokeratin 18 Antibody (DE-K18).")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (DE-K18) [NBP2-44947] - Confocal Immunofluorescence image of HeLa cells using Cytokeratin 18 Antibody (DE-K18) Green (CF488) and Red Dot is used to label the nuclei.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-DE-K18-Immunocytochemistry-Immunofluorescence-NBP2-44947-img0006.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (DE-K18) [NBP2-44947] - Human Colon Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (DE-K18).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-DE-K18-Immunohistochemistry-Paraffin-NBP2-44947-img0001.jpg)
![Flow Cytometry: Cytokeratin 18 Antibody (DE-K18) [NBP2-44947] - Flow Cytometric Analysis of trypsinised MeOH-fixed HeLa cells using Cytokeratin 18 Antibody (DE-K18) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-DE-K18-Flow-Cytometry-NBP2-44947-img0005.jpg)
![Western Blot: Cytokeratin 18 Antibody (DE-K18) [NBP2-44947] - Western Blot Analysis of MCF-7cell lysate using Cytokeratin 18 Antibody (DE-K18).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-DE-K18-Western-Blot-NBP2-44947-img0007.jpg)
![Simple Western: Cytokeratin 18 Antibody (DE-K18) [NBP2-44947] - Simple Western lane view shows a specific band for Cytokeratin 18 in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-DE-K18-Simple-Western-NBP2-44947-img0002.jpg)
![Simple Western: Cytokeratin 18 Antibody (DE-K18) [NBP2-44947] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 18 antibody was used at 10 ug/ml dilution of HeLa lysates(s) respectively.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-DE-K18-Simple-Western-NBP2-44947-img0003.jpg)
![Western Blot: Cortactin Antibody [NBP2-15971] - Wild-type (WT) and Cortactin knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1], N-term. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cortactin-Antibody-Western-Blot-NBP2-15971-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Cortactin Antibody [NBP2-15971] - Wild-type (WT) and Cortactin knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1], N-term. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: Cortactin Antibody [NBP2-15971] - Sample (30 ug of whole cell lysate) A: PC-12 7. 5% SDS PAGE gel, diluted at 1:3000.](http://images.novusbio.com/fullsize/Cortactin-Antibody-Western-Blot-NBP2-15971-img0003.jpg)
![Immunohistochemistry-Paraffin: Cortactin Antibody [NBP2-15971] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/Cortactin-Antibody-Immunohistochemistry-Paraffin-NBP2-15971-img0004.jpg)
![Western Blot: Cortactin Antibody [NBP2-15971] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Cortactin-Antibody-Western-Blot-NBP2-15971-img0002.jpg)
![Immunohistochemistry-Paraffin: Cortactin Antibody [NBP2-15971] - Cortactin antibody [N1], N-term detects Cortactin protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.Cortactin stained by Cortactin antibody [N1], N-term (NBP2-15971) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15971_rabbit-polyclonal-cortactin-antibody-910202420131266.jpg)
![Immunocytochemistry/ Immunofluorescence: Cortactin Antibody [NBP2-15971] - Cortactin antibody [N1], N-term detects Cortactin protein at cytoplasm and cell membrane by immunofluorescent analysis.Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cortactin stained by Cortactin antibody [N1], N-term (NBP2-15971) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp2-15971_rabbit-polyclonal-cortactin-antibody-91020242014648.jpg)
![Western Blot: Cortactin Antibody [NBP2-15971] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1], N-term (NBP2-15971) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15971_rabbit-polyclonal-cortactin-antibody-91020242075994.jpg)
![Western Blot: Cortactin Antibody [NBP2-15971] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1], N-term (NBP2-15971) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15971_rabbit-polyclonal-cortactin-antibody-91020242041243.jpg)
![Western Blot: MCM2 Antibody (6A8) [H00004171-M01] - Analysis of MCM2 expression in transfected 293T cell line by MCM2 monoclonal antibody (M01), clone 6A8.Lane 1: MCM2 transfected lysate(101.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MCM2-Antibody-6A8-Western-Blot-H00004171-M01-img0011.jpg "Western Blot: MCM2 Antibody (6A8) [H00004171-M01] - Analysis of MCM2 expression in transfected 293T cell line by MCM2 monoclonal antibody (M01), clone 6A8.Lane 1: MCM2 transfected lysate(101.9 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: MCM2 Antibody (6A8) [H00004171-M01] - Analysis of monoclonal antibody to MCM2 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/MCM2-Antibody-6A8-Immunocytochemistry-Immunofluorescence-H00004171-M01-img0007.jpg)
![Western Blot: MCM2 Antibody (6A8) [H00004171-M01] - MCM2 monoclonal antibody (M01), clone 6A8 Analysis of MCM2 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/MCM2-Antibody-6A8-Western-Blot-H00004171-M01-img0010.jpg)
![Immunoprecipitation: MCM2 Antibody (6A8) [H00004171-M01] - Analysis of MCM2 transfected lysate using anti-MCM2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MCM2 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/MCM2-Antibody-6A8-Immunoprecipitation-H00004171-M01-img0008.jpg)
![ELISA: MCM2 Antibody (6A8) [H00004171-M01] - Detection limit for recombinant GST tagged MCM2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MCM2-Antibody-6A8-ELISA-H00004171-M01-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: TGN46 Antibody (2F11) [H00010618-M02] - Analysis of monoclonal antibody to TGOLN2 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/TGN46-Antibody-2F11-Immunocytochemistry-Immunofluorescence-H00010618-M02-img0003.jpg "Immunocytochemistry/Immunofluorescence: TGN46 Antibody (2F11) [H00010618-M02] - Analysis of monoclonal antibody to TGOLN2 on HeLa cell . Antibody concentration 10 ug/ml.")
![Immunohistochemistry-Paraffin: TGN46 Antibody (2F11) [H00010618-M02] - Analysis of monoclonal antibody to TGOLN2 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/TGN46-Antibody-2F11-Immunohistochemistry-Paraffin-H00010618-M02-img0005.jpg)
![ELISA: TGN46 Antibody (2F11) [H00010618-M02] - Detection limit for recombinant GST tagged TGOLN2 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/TGN46-Antibody-2F11-ELISA-H00010618-M02-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody [NBP3-05771] - Caco-2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- EAAT1/GLAST-1/SLC1A3 Antibody NBP3-05771 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05771-img0004.jpg "Immunocytochemistry/Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody [NBP3-05771] - Caco-2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- EAAT1/GLAST-1/SLC1A3 Antibody NBP3-05771 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody [NBP3-05771] - Analysis of a FFPE tissue section of mouse brain using 1:200 dilution of SLC1A3 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. Strong cytoplasmic and membrane reactivity were observed.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Immunohistochemistry-Paraffin-NBP3-05771-img0001.jpg)
![Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NBP3-05771] - An intracellular stain was performed on Hek293 cells with SLC1A3 Antibody NBP3-05771 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Flow-Cytometry-NBP3-05771-img0003.jpg)
![Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NBP3-05771] - An intracellular stain was performed on U937 cells with SLC1A3 Antibody NBP3-05771 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Flow-Cytometry-NBP3-05771-img0002.jpg)
![Western Blot: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Whole cell lysate (5 ug) from EL4, mIMCD-3, NIH 3T3,Renca, and TCMK-1 cells prepared using NETN lysis buffer.Antibody: Rabbit anti-E-Cadherin recombinant monoclonalantibody [BLR088G] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Western-Blot-NBP3-14687-img0007.jpg "Western Blot: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Whole cell lysate (5 ug) from EL4, mIMCD-3, NIH 3T3,Renca, and TCMK-1 cells prepared using NETN lysis buffer.Antibody: Rabbit anti-E-Cadherin recombinant monoclonalantibody [BLR088G] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG Chemiluminescence with an exposure time of 10 seconds.")
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G] . Secondary:HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Immunocytochemistry-Immunofluorescence-NBP3-14687-img0008.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G]. Secondary: HRPconjugated goat anti-rabbit IgG. DAB.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Immunohistochemistry-Paraffin-NBP3-14687-img0004.jpg)
![Western Blot: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Whole cell lysate (5 ug) from GaMG, HCT 116, U2OS,OVCAR-4, Jurkat, 22Rv1, HeLa, MCF-7, and K-562 cellsprepared using NETN lysis buffer. Antibody: Rabbit anti-ECadherin recombinant monoclonal antibody [BLR088G] used at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 10 seconds.Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Western-Blot-NBP3-14687-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Immunocytochemistry-Immunofluorescence-NBP3-14687-img0005.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Immunohistochemistry-Paraffin-NBP3-14687-img0002.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Immunohistochemistry-Paraffin-NBP3-14687-img0003.jpg)
![Immunoprecipitation: E-Cadherin Antibody [NBP3-14687] - Whole cell lysate(1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer.Antibodies: Rabbit anti-E-Cadherin recombinant monoclonal antibodyused for IP at 20 ul/mg lysate. E-Cadherin was also immunoprecipitated by rabbit anti-E-Cadherin antibody. For blotting immunoprecipitated E-Cadherin, was used at 1:1000.Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-Immunoprecipitation-NBP3-14687-img0001.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Analysis in human parathyroid gland and skeletal muscle tissues using NBP2-38622 antibody. Corresponding CASR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Analysis in human parathyroid gland and skeletal muscle tissues using NBP2-38622 antibody. Corresponding CASR RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human kidney, liver, pancreas and parathyroid gland using Anti-CASR antibody NBP2-38622 (A) shows similar protein distribution across tissues to independent antibody NBP1-84687 (B).](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0012.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Analysis in control (vector only transfected HEK293T lysate) and CASR over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Western-Blot-NBP2-38622-img0006.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0017.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human parathyroid gland shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0014.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human kidney shows moderate membranous positivity in cells in distal tubules.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0015.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human pancreas shows weak to moderate membranous positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0016.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0018.jpg)
![Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Staining of human cerebral cortex, liver, placenta and testis using Anti-Pericentrin antibody NBP1-87772 (A) shows similar protein distribution across tissues to independent antibody NBP1-87771 (B).](http://images.novusbio.com/fullsize/Pericentrin-Antibody-Immunohistochemistry-Paraffin-NBP1-87772-img0017.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Staining of human cerebral cortex, liver, placenta and testis using Anti-Pericentrin antibody NBP1-87772 (A) shows similar protein distribution across tissues to independent antibody NBP1-87771 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Pericentrin Antibody [NBP1-87772] - Smear of -20C methanol-fixed mouse small intestinal epithelial cells stained for ZO-1 (red) and pericentrin (green). ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Pericentrin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87772-img0019.jpg)
![Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Pericentrin-Antibody-Immunohistochemistry-Paraffin-NBP1-87772-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Pericentrin Antibody [NBP1-87772] - Staining of human cell line A-431 shows localization to centrosome. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Pericentrin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87772-img0006.jpg)
![Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/Pericentrin-Antibody-Immunohistochemistry-Paraffin-NBP1-87772-img0009.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Staining in human testis and liver tissues using anti-PCNT antibody. Corresponding PCNT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Pericentrin-Antibody-Immunohistochemistry-Paraffin-NBP1-87772-img0010.jpg)
![Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Pericentrin-Antibody-Immunohistochemistry-Paraffin-NBP1-87772-img0015.jpg)
![Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP1-84584] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-Immunohistochemistry-Paraffin-NBP1-84584-img0013.jpg "Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP1-84584] - Staining of human skeletal muscle shows no positivity in myocytes as expected.")
![Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP1-84584] - Staining of human breast cancer shows no positivity in tumor cells as expected.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-Immunohistochemistry-Paraffin-NBP1-84584-img0010.jpg)
![Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP1-84584] - Staining of human breast cancer shows strong membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-Immunohistochemistry-Paraffin-NBP1-84584-img0011.jpg)
![Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP1-84584] - Staining of human kidney shows weak to moderate granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-Immunohistochemistry-Paraffin-NBP1-84584-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP3-18057] - U-251 MG cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD4 Antibody (NBP3-18057) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/CD4-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-18057-img0002.jpg "Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP3-18057] - U-251 MG cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD4 Antibody (NBP3-18057) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: CD4 Antibody [NBP3-18057] - Analysis of a FFPE tissue section of human spleen using 1:200 dilution of CD4 antibody (NBP3-18057). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/CD4-Antibody-Immunohistochemistry-Paraffin-NBP3-18057-img0001.jpg)
![Flow Cytometry: CD4 Antibody [NBP3-18057] - An intracellular stain was performed on Jurkat cells with CD4 Antibody NBP3-18057 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/CD4-Antibody-Flow-Cytometry-NBP3-18057-img0003.jpg)
![Immunohistochemistry-Paraffin: Rabbit CD4 pAb [NBP3-18057] - Immunofluorescence staining of human tonsil FFPE tissue. Antibody diluted 1:50 (Catalog # NBP3-18057AF647) in 3% BSA and incubated over night at 4 °C. Heat mediated antigen retrieval at pH 9. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp3-18057_rabbit-cd4-pab-immunohistochemistry-paraffin-49202411280..jpg)
![Western Blot: SMAC/Diablo Antibody [NBP1-76772] - Western Blot Validation in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: SMAC/Diablo NBP1-76772 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/SMAC-Diablo-Antibody-Western-Blot-NBP1-76772-img0006.jpg "Western Blot: SMAC/Diablo Antibody [NBP1-76772] - Western Blot Validation in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: SMAC/Diablo NBP1-76772 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Immunohistochemistry-Paraffin: SMAC/Diablo Antibody [NBP1-76772] - Immunohistochemical staining of mouse spleen tissue using Smac antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/SMAC-Diablo-Antibody-Immunohistochemistry-Paraffin-NBP1-76772-img0002.jpg)
![Western Blot: SMAC/Diablo Antibody [NBP1-76772] - Analysis of Smac in mouse heart tissue lysate in the absence (A) or presence (B) of blocking peptide and in rat heart tissue lysate with Smac antibody at 1 ug /ml.](http://images.novusbio.com/fullsize/SMAC-Diablo-Antibody-Western-Blot-NBP1-76772-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: SMAC/Diablo Antibody [NBP1-76772] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane. Antibodies: SMAC/Diablo NB100-55900 (1 ug/mL), SMAC/Diablo NBP1-76772 (1 ug/mL), and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/SMAC-Diablo-Antibody-Western-Blot-NBP1-76772-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: SMAC/Diablo Antibody - BSA Free [NBP1-76772] - Figure 4 Immunofluorescence Validation of SMAC/Diablo in Mouse Spleen CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed Mouse Spleen Cells labeling SMAC/Diablo with at 10 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-76772_rabbit-polyclonal-smac-diablo-antibody-271120242022953.jpg)
![Immunohistochemistry: SMAC/Diablo Antibody - BSA Free [NBP1-76772] - Figure 5 Immunohistochemistry Validation of SMAC/Diablo in Mouse Spleen Tissue Immunohistochemical analysis of paraffin-embedded Mouse Spleen Tissue using anti-SMAC/Diablo antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76772_rabbit-polyclonal-smac-diablo-antibody-271120242004138.jpg)
![Western Blot: L1CAM Antibody (OTI2A6) [NBP2-02149] - Analysis of extracts (35ug) from 9 different cell lines by using anti-L1CAM monoclonal antibody.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Western-Blot-NBP2-02149-img0012.jpg "Western Blot: L1CAM Antibody (OTI2A6) [NBP2-02149] - Analysis of extracts (35ug) from 9 different cell lines by using anti-L1CAM monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY L1CAM.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Immunocytochemistry-Immunofluorescence-NBP2-02149-img0015.jpg)
![Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of paraffin-embedded pancreas tissue using anti-L1CAMmouse monoclonal antibody.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Immunohistochemistry-Paraffin-NBP2-02149-img0010.jpg)
![Flow Cytometry: L1CAM Antibody (OTI2A6) [NBP2-02149] - Analysis of Jurkat cells, using anti-L1CAM antibody, (Red) compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Flow-Cytometry-NBP2-02149-img0002.jpg)
![Western Blot: L1CAM Antibody (OTI2A6) [NBP2-02149] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY L1CAM (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-L1CAM.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Western-Blot-NBP2-02149-img0003.jpg)
![Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of paraffin-embedded endometrium tissue within the normal limits using anti-L1CAM mouse monoclonal antibody. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Immunohistochemistry-Paraffin-NBP2-02149-img0017.jpg)
![Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-L1CAMmouse monoclonal antibody.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Immunohistochemistry-Paraffin-NBP2-02149-img0004.jpg)
![Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-L1CAM mouse monoclonal antibody.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Immunohistochemistry-Paraffin-NBP2-02149-img0005.jpg)
![Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of paraffin-embedded breast tissue using anti-L1CAM mouse monoclonal antibody.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Immunohistochemistry-Paraffin-NBP2-02149-img0006.jpg)
![Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of paraffin-embedded colon tissue using anti-L1CAM mouse monoclonal antibody.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Immunohistochemistry-Paraffin-NBP2-02149-img0007.jpg)
![Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of paraffin-embedded Kidney tissue using anti-L1CAM mouse monoclonal antibody.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Immunohistochemistry-Paraffin-NBP2-02149-img0008.jpg)
![Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of paraffin-embedded Ovary tissue using anti-L1CAM mouse monoclonal antibody.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Immunohistochemistry-Paraffin-NBP2-02149-img0009.jpg)
![Flow Cytometry: L1CAM Antibody (OTI2A6) [NBP2-02149] - Analysis of Hela cells, using anti-L1CAM antibody, (Red) compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Flow-Cytometry-NBP2-02149-img0001.jpg)
![Immunoprecipitation: L1CAM Antibody (OTI2A6) [NBP2-02149] - Immunoprecipitation of L1CAM by using TrueMab monoclonal anti-L1CAM antibody (Negative control: IP without adding anti-L1CAM antibody). For each experiment, 500ul of DDK tagged L1CAM overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-L1CAM antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Immunoprecipitation-NBP2-02149-img0018.jpg)