| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59339-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61121-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-21805-0.1ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97472-0.2mg | |||
| BSA Free | NBP1-97472-0.025mg | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Cy3 | NB120-11319 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-1393-0.025mg | |||
![Western Blot: HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - Analysis of Human Heat shocked HeLa cell lysates showing detection of HSP70 protein using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11. Lane 1: Molecular Weight ladder (MW). Lane 2: HeLa cell lysates. Load: 20 ug. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:1000.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-1H11-Western-Blot-NBP2-59339-img0004.jpg "Western Blot: HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - Analysis of Human Heat shocked HeLa cell lysates showing detection of HSP70 protein using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11. Lane 1: Molecular Weight ladder (MW). Lane 2: HeLa cell lysates. Load: 20 ug. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:1000.")
![Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - Analysis on non-permeabilized HCT116 cells using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11: FITC conjugate showing cell membrane staining. Tissue: HCT116 cells. Species: Human. Fixation: 4% Formaldehyde. Permeabilization: None. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:100. Counterstain: Wheat germ agglutinin Texas red membrane marker; DAPI (blue) nuclear stain. Localization: Cell surface, cell membrane. (A) DAPI nuclear stain. (B) Wheat germ agglutinin Texas red. (C) HSP70 Antibody. (D) Composite. Courtesy of: Lawrence Hightower, Charles Giardina, and Didem Ozcan from University of Connecticut.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-1H11-Immunocytochemistry-Immunofluorescence-NBP2-59339-img0006.jpg)
![Flow (Cell Surface): HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - A cell surface stain was performed on HeLa cells with Hsp70 / Hsc70 Antibody (1H11) NBP2-59339PE (blue) and a matched isotype control(orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-1H11-Flow-Cell-Surface-NBP2-59339-img0007.jpg)
![Flow Cytometry: HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - Hsp70 / Hsc70 Antibody (1H11) [NBP2-59339] - Clone 1H11: FITC conjugate. Tissue: Jurkat E6.1. Species: Human. Unfixed/non-permeablized cells were incubated with (A) Anti-HSP70 control antibody (not cell-surface-specific), or (B) Anti-HSP70 antibody, clone 1H11 for 40 min at 4 degrees C. Fixed/permeabilized cells were incubated with (C) Anti-HSP70 control antibody, or (D) Anti-HSP70 antibody, clone 1H11 or 40 min at 4 degrees C. Analysis shows that non-permeabilized cells show cell surface binding with 1H11 clone (NBP2-59339) but not with the control antibody, and the permeabilized cells show strong intracellular binding with the control antibody but much less with 1H11 clone (NBP2-59339). Courtesy of: Dr. Elyse Ireland, Institute of Medicine, University of Chester.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-1H11-Flow-Cytometry-NBP2-59339-img0002.jpg)
![Western Blot: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Western blot analysis of Human, Mouse brain lysate showing detection of ~16 kDa alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human brain lysate. Lane 3: Mouse brain lysate. Lane 4: Alpha Synuclein Monomer (0.5 ug). Load: 15 ug. Block: 2.5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16 kDa. Other Band(s): 100, 75, 45, 30,16 kDa.](http://images.novusbio.com/fullsize/alpha-Synuclein-[p-Ser129]-Antibody-Western-Blot-NBP2-61121-img0004.jpg "Western Blot: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Western blot analysis of Human, Mouse brain lysate showing detection of ~16 kDa alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human brain lysate. Lane 3: Mouse brain lysate. Lane 4: Alpha Synuclein Monomer (0.5 ug). Load: 15 ug. Block: 2.5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16 kDa. Other Band(s): 100, 75, 45, 30,16 kDa.")
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Phospho serine 129 antibody (NBP2-61121) was used to detect phosphorylated alpha synuclein in primary mouse hippocampal neurons treated with 100 nM sonicated mouse alpha synuclein PFFs (A). Phosphorylated alpha synuclein was visible in perinucleus and neurites compared to untreated control (B). Read the protocol at pabmabs.com/?p=7901. Image courtesy of Trine Rasmussen, Simon Molgaard Jensen at Aarhus University.](http://images.novusbio.com/fullsize/alpha-Synuclein-[p-Ser129]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-61121-img0006.jpg)
![Immunohistochemistry: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Immunohistochemistry analysis using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.](http://images.novusbio.com/fullsize/alpha-Synuclein-[p-Ser129]-Antibody-Immunohistochemistry-NBP2-61121-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Tissue: Primary hippocampal neurons treated with active Alpha Synuclein Protein Aggregate (SPR-322) at 4 g/ml to induce fibrils. Species: Rat. Fixation: 4% paraformaldehyde. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:500 for 24 hours at 4C. Secondary Antibody: Goat Anti-Rabbit Alexa Fluor 488 at 1:700 for 1 hour at RT. Counterstain: Guinea Pig Anti-NeuN (red) neuronal marker (Donkey Anti-Guinea Pig Alexa Fluor 647 1:700); Hoechst (blue) nuclear stain at 1:6000, 1:3000 for 60 min at RT, 5 min at RT. Magnification: 20X.](http://images.novusbio.com/fullsize/alpha-Synuclein [p Ser129] Antibody-Immunocytochemistry-Immunofluorescence-NBP2-61121-img0007.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free [NBP2-70204] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY liver Arginase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-liver A](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-OTI4E6-Azide-and-BSA-Free-Western-Blot-NBP2-70204-img0003.jpg "Western Blot: Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free [NBP2-70204] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY liver Arginase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-liver A")
![Immunohistochemistry: Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free [NBP2-70204] - Staining of paraffin-embedded Human liver tissue using anti-liver Arginase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-OTI4E6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70204-img0005.jpg)
![Flow Cytometry: Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free [NBP2-70204] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-liver Arginase antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-OTI4E6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70204-img0004.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free [NBP2-70204] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using antiARG1 monoclonal antibody.(1:200)](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-OTI4E6-Azide-and-BSA-Free-Western-Blot-NBP2-70204-img0001.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody (OTI4E6) - Azide and BSA Free [NBP2-70204] - Analysis of extracts (35ug) from 9 different cell lines by using anti-liver Arginase monoclonal antibody.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-OTI4E6-Azide-and-BSA-Free-Western-Blot-NBP2-70204-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (GC-4) [NBP2-21805] - HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with Monoclonal Anti-N-Cadherin, Clone: GC-4. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-GC-4-Immunofluorescence-NBP2-21805-img0002.jpg "Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (GC-4) [NBP2-21805] - HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with Monoclonal Anti-N-Cadherin, Clone: GC-4. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.")
![Immunohistochemistry: N-Cadherin Antibody (GC-4) [NBP2-21805] - Frozen acetone fixed chicken cardiac sections stained with Monoclonal Anti-N-Cadherin antibody produced in Mouse clone GC-4: followed by Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-GC-4-Immunohistochemistry-NBP2-21805-img0001.jpg)
![Western Blot: Transferrin Antibody [NBP1-97472] - Transferrin blot (C) or Ponceau S staining (D) was used as loading control. **P < 0.01 (two-tailed unpaired t test), n = 3 mice per group; values represent the mean +/- SEM. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33087487) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Transferrin-Antibody-Western-Blot-NBP1-97472-img0002.jpg "Western Blot: Transferrin Antibody [NBP1-97472] - Transferrin blot (C) or Ponceau S staining (D) was used as loading control. **P < 0.01 (two-tailed unpaired t test), n = 3 mice per group; values represent the mean +/- SEM. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33087487) licensed under a CC-BY license.")
![Western Blot: Transferrin Antibody [NBP1-97472] - Analysis of rat apotransferrin protein probed with transferrin pAb.](http://images.novusbio.com/fullsize/Transferrin-Antibody-Western-Blot-NBP1-97472-img0001.jpg)
![Western Blot: Transferrin Antibody [NBP1-97472] - Transfer of hippocampal/hypothalamic PTMS to other brain regions & the blood.(A, B) 3-mo male C57BL/6 mice were injected with a dose of synapsin promoter-driven PTMS-HA lentivirus in one side of the posterior hypothalamus (A) or both sides of the hippocampus targeting CA1 & dentate gyrus (B). At 2 wk post injection, brain sections were immunostained for HA in the injected regions versus other brain areas. Small arrows pointed to some HA positive cells in transfer brain regions. Scale bar, 100 μm. Additional information of control lentivirus injections are presented in Fig S6. (C, D) 3-mo male C57BL/6 mice received bilateral injections of neuron-specific lentivirus of synapsin promoter-driven PTMS-HA (labelled as “PTMS-HA”) versus Control-HA (labelled as “Con-HA”) in the hippocampus (targeting CA1 & dentate gyrus) & posterior hypothalamus, & at 2-wk post injection, secretion of PTMS-HA to the CSF (C) & the serum (D) was assessed via IP-Western blotting. (C, D) Transferrin blot (C) or Ponceau S staining (D) was used as loading control. **P < 0.01 (two-tailed unpaired t test), n = 3 mice per group; values represent the mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33087487), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97472_rabbit-polyclonal-transferrin-antibody-31020241612216.jpg)
![Western Blot: Transferrin Antibody [NBP1-97472] - PTMS secretion declines during aging.(A, B) PTMS levels in the CSF of male C57BL/6 mice at young (∼4 mo) or old (∼24 mo) age were measured by Western blot (A) which were quantified in panel (B). Transferrin was used to as a loading control. (C) Young (3-mo) or middle aged (18-mo) male C57BL/6 mice received unilateral injection of the same dose of Sox2 promoter-driven PTMS-HA lentivirus in the mediobasal hypothalamus. White arrows point to the site of injections. At 1 wk postinjection, hypothalamic sections were generated for HA immunostaining to show the levels of both primary expression of PTMS-HA in Sox2-positive cells & secondary transfer of PTMS-HA into Sox2-negative cells. Sox2 co-immunostaining was performed to reveal Sox2-positive cells in this region. Lower panels in show high-magnification views of outlined areas. Scale bar, 100 μm. 3V: hypothalamic third ventricle. *P < 0.05, two-tailed unpaired t test (B), n = 3 mice per group; values represent the mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33087487), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97472_rabbit-polyclonal-transferrin-antibody-3102024161227.jpg)
![Western Blot: Transferrin Antibody [NBP1-97472] - Contribution of the hypothalamus to PTMS in the CSF.(A) PTMS levels in blood serum versus CSF were detected by immunoprecipitation (IP)-Western blot (left panel), & the band intensities (au, arbitrary unit) were quantified (right panel). Matched IgG was used as a negative control of IP procedure, & Commassie staining was used as a loading control. (B, C) Injection of ptms-shRNA (sh-ptms) or scramble control shRNA (sh-control) lentiviruses into hypothalamic third ventricle of 3-mo male C57BL/6 mice (B), & at 1 mo postinjection, PTMS levels in CSF were measured by Western blot (left panel, transferrin as a loading control) & quantified (right panel). Scale bar, 100 μm. (A, C) *P < 0.05 (two-tailed unpaired t test), n = 4 mice (A) or 3 mice (C) per group; values represent the mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33087487), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97472_rabbit-polyclonal-transferrin-antibody-31020241612226.jpg)
![Western Blot: gamma Tubulin Antibody [NB120-11318] - Cell lysates (lanes 1-7) were separated on SDS-PAGE, blotted with 0.7 ug/mL Anti-gamma-Tubulin, and developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. U87; 2. HeLa; 3. Jurkat; 4. C2C12; 5. Rat2; 6. MDCK; 7. A431](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-Western-Blot-NB120-11318-img0015.jpg "Western Blot: gamma Tubulin Antibody [NB120-11318] - Cell lysates (lanes 1-7) were separated on SDS-PAGE, blotted with 0.7 ug/mL Anti-gamma-Tubulin, and developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. U87; 2. HeLa; 3. Jurkat; 4. C2C12; 5. Rat2; 6. MDCK; 7. A431")
![Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody [NB120-11318] - Centrosomal localization of gamma-tubulin in Rat2 cells. Fixed cells were stained with 0.8 ug/mL Anti-gamma-Tubulin, followed by Goat Anti-Rabbit IgG, Atto448 dye conjugate (x100 magnification). Actin staining with phalloidin rhodamine conjugate. Nuclear staining with DAPI.](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11318-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody [NB120-11318] - Centrosomal localization of gamma-tubulin in NIH3T3 cells. Fixed cells were stained with 0.8 ug/mL Anti-gamma-Tubulin, followed by Goat Anti-Rabbit IgG, Atto448 dye conjugate (x100 magnification). Actin staining with phalloidin rhodamine conjugate. Nuclear staining with DAPI.](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11318-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody [NB120-11318] - Centrosomal localization of gamma-tubulin in NIH3T3 cells. Fixed cells were stained with 0.8 ug/mL Anti-gamma-Tubulin, followed by Goat Anti-Rabbit IgG, Atto448 dye conjugate (x100 magnification). Actin staining with phalloidin rhodamine conjugate. Nuclear staining with DAPI.](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11318-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody [NB120-11318] - Centrosomal localization of gamma-tubulin in HeLa cells. Fixed cells were stained with 0.8 ug/mL Anti-gamma-Tubulin, followed by Goat Anti-Rabbit IgG, Atto448 dye conjugate (x100 magnification). Actin staining with phalloidin rhodamine conjugate. Nuclear staining with DAPI.](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11318-img0013.jpg)
![Western Blot: ADAM8 Antibody [NB600-1393] - analysis of ADAM8 in PSN-1 whole cell lysate using anti-ADAM8 antibody. The primary antibody was used at a dilution of 1:1000, incubated overnight at 4C. Image from verified customer review.](http://images.novusbio.com/fullsize/ADAM8-Antibody-Western-Blot-NB600-1393-img0001.jpg "Western Blot: ADAM8 Antibody [NB600-1393] - analysis of ADAM8 in PSN-1 whole cell lysate using anti-ADAM8 antibody. The primary antibody was used at a dilution of 1:1000, incubated overnight at 4C. Image from verified customer review.")
![Western Blot: p63/TP73L Antibody (SC06-31) [NBP3-32703] - Western blot analysis of p63/TP73L on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (NBP3-32703, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32703_rabbit-p63-tp73l-mab-sc06-31-27820241631652.jpg "Western Blot: p63/TP73L Antibody (SC06-31) [NBP3-32703] - Western blot analysis of p63/TP73L on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (NBP3-32703, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p63/TP73L Antibody (SC06-31) [NBP3-32703] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-p63/TP73L antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32703, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32703_rabbit-p63-tp73l-mab-sc06-31-298202413585578.jpg)
![Immunocytochemistry/ Immunofluorescence: p63/TP73L Antibody (SC06-31) [NBP3-32703] - ICC staining of p63/TP73L in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP3-32703, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32703_rabbit-p63-tp73l-mab-sc06-31-2920241192350.jpg)
![Flow Cytometry: p63/TP73L Antibody (SC06-31) [NBP3-32703] - Flow cytometric analysis of p63/TP73L was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (NBP3-32703, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32703_rabbit-p63-tp73l-mab-sc06-31-29202416453240.jpg)
![Western Blot: Prohibitin 2 Antibody (JE35-46) [NBP3-32900] - Western blot analysis of Prohibitin 2 on different lysates with Rabbit anti-Prohibitin 2 antibody (NBP3-32900) at 1/1,000 dilution. Lane 1: Huh7 cell lysate (15 ug/Lane) Lane 2: HeLa cell lysate (15 ug/Lane) Lane 3: HepG2 cell lysate (15 ug/Lane) Lane 4: PANC-1 cell lysate (15 ug/Lane) Lane 5: RAW264.7 cell lysate (15 ug/Lane) Lane 6: NIH/3T3 cell lysate (15 ug/Lane) Lane 7: PC-12 cell lysate (15 ug/Lane) Lane 8: Mouse liver tissue lysate (30 ug/Lane) Lane 9: Rat liver tissue lysate (30 ug/Lane) Lane 10: Mouse heart tissue lysate (30 ug/Lane) Lane 11: Rat heart tissue lysate (30 ug/Lane) Lane 12: Mouse testis tissue lysate (30 ug/Lane) Lane 13: Rat testis tissue lysate (30 ug/Lane) Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32900) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32900_rabbit-prohibitin-2-mab-je35-46-288202412461642.jpg "Western Blot: Prohibitin 2 Antibody (JE35-46) [NBP3-32900] - Western blot analysis of Prohibitin 2 on different lysates with Rabbit anti-Prohibitin 2 antibody (NBP3-32900) at 1/1,000 dilution. Lane 1: Huh7 cell lysate (15 ug/Lane) Lane 2: HeLa cell lysate (15 ug/Lane) Lane 3: HepG2 cell lysate (15 ug/Lane) Lane 4: PANC-1 cell lysate (15 ug/Lane) Lane 5: RAW264.7 cell lysate (15 ug/Lane) Lane 6: NIH/3T3 cell lysate (15 ug/Lane) Lane 7: PC-12 cell lysate (15 ug/Lane) Lane 8: Mouse liver tissue lysate (30 ug/Lane) Lane 9: Rat liver tissue lysate (30 ug/Lane) Lane 10: Mouse heart tissue lysate (30 ug/Lane) Lane 11: Rat heart tissue lysate (30 ug/Lane) Lane 12: Mouse testis tissue lysate (30 ug/Lane) Lane 13: Rat testis tissue lysate (30 ug/Lane) Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32900) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Prohibitin 2 Antibody (JE35-46) [NBP3-32900] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Prohibitin 2 antibody (NBP3-32900) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32900) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32900_rabbit-prohibitin-2-mab-je35-46-298202414262693.jpg)
![Immunocytochemistry/ Immunofluorescence: Prohibitin 2 Antibody (JE35-46) [NBP3-32900] - Immunocytochemistry analysis of HeLa cells labeling Prohibitin 2 with Rabbit anti-Prohibitin 2 antibody (NBP3-32900) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Prohibitin 2 antibody (NBP3-32900) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32900_rabbit-prohibitin-2-mab-je35-46-2920241192365.jpg)
![Flow Cytometry: Prohibitin 2 Antibody (JE35-46) [NBP3-32900] - Flow cytometric analysis of HeLa cells labeling Prohibitin 2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32900, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32900_rabbit-prohibitin-2-mab-je35-46-29202416453249.jpg)
![Western Blot: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of Chymase/CMA1/Mast Cell on different lysates with Rabbit anti-Chymase/CMA1/Mast Cell antibody at 1/500 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Rat spleen tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-JB74-32-Western-Blot-NBP2-75441-img0006.jpg "Western Blot: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of Chymase/CMA1/Mast Cell on different lysates with Rabbit anti-Chymase/CMA1/Mast Cell antibody at 1/500 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Rat spleen tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-Chymase/CMA1/Mast Cell Chymase antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-JB74-32-Immunohistochemistry-Paraffin-NBP2-75441-img0008.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of paraffin-embedded human tonsil tissue using anti-Mast Cell Chymase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-JB74-32-Immunohistochemistry-Paraffin-NBP2-75441-img0004.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Human small intestine tissue using anti-Mast Cell Chymase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-JB74-32-Immunohistochemistry-Paraffin-NBP2-75441-img0005.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of paraffin-embedded human skin tissue using anti-Mast Cell Chymase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-JB74-32-Immunohistochemistry-Paraffin-NBP2-75441-img0003.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of paraffin-embedded human small intestine tissue with Rabbit anti-Chymase/CMA1/Mast Cell Chymase antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-JB74-32-Immunohistochemistry-Paraffin-NBP2-75441-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: RPSA Antibody (RP-01) [NBP3-09109] - Staining of RPSA in human glioblastoma cells T98G using mouse monoclonal antibody RP-01. The cells were fixed with paraformaldehyde and permeabilized using Triton X-100.](http://images.novusbio.com/fullsize/RPSA-Antibody-RP-01-Immunocytochemistry-Immunofluorescence-NBP3-09109-img0001.jpg "Immunocytochemistry/Immunofluorescence: RPSA Antibody (RP-01) [NBP3-09109] - Staining of RPSA in human glioblastoma cells T98G using mouse monoclonal antibody RP-01. The cells were fixed with paraformaldehyde and permeabilized using Triton X-100.")
![Western Blot: Mouse Monoclonal RPSA Antibody (RP-01) [NBP3-09109] - Western blotting analysis was performed on urea buffer extracts of Ramos, Raji, HeLa, Jurkat, and Huvec cells mixed with hot reducing SDS-loading buffer. Samples were resolved using 10% SDS-PAGE gel.Nitrocellulose membrane blot was probed with mouse IgG2a monoclonal antibody RP-01 (2 ug/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed.RPSA was detected at ~37 kDa in all tested cell lines.](http://images.novusbio.com/fullsize/antibody/nbp3-09109_mouse-monoclonal-rpsa-antibody-rp-01-sandwich-elisa-151202513658..jpg)
![Immunoprecipitation: PCNA Antibody (A6-G11-R) [NBP3-32725] - PCNA was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32725 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32725 at 1/10,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32725 IP in HeLa cell lysate Lane 3: Mouse IgG instead of NBP3-32725 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds; ECL](http://images.novusbio.com/fullsize/nbp3-32725_mouse-pcna-mab-a6-g11-r-2682024158269.jpg "Immunoprecipitation: PCNA Antibody (A6-G11-R) [NBP3-32725] - PCNA was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32725 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32725 at 1/10,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32725 IP in HeLa cell lysate Lane 3: Mouse IgG instead of NBP3-32725 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds; ECL")
![Immunohistochemistry: PCNA Antibody (A6-G11-R) [NBP3-32725] - Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Mouse anti-PCNA antibody (NBP3-32725) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32725) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32725_mouse-pcna-mab-a6-g11-r-2982024149267.jpg)
![Immunocytochemistry/ Immunofluorescence: PCNA Antibody (A6-G11-R) [NBP3-32725] - Immunocytochemistry analysis of HeLa cells labeling PCNA with Mouse anti-PCNA antibody (NBP3-32725) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PCNA antibody (NBP3-32725) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32725_mouse-pcna-mab-a6-g11-r-29202410484872.jpg)
![Western Blot: PCNA Antibody (A6-G11-R) [NBP3-32725] - Western blot analysis of PCNA on different lysates with Mouse anti-PCNA antibody (NBP3-32725) at 1/20,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: HEK-293 cell lysate (15 µg/Lane) Lane 3: HCT 116 cell lysate (15 µg/Lane) Lane 4: K-562 cell lysate (15 µg/Lane) Lane 5: RAW264.7 cell lysate (15 µg/Lane) Lane 6: C2C12 cell lysate (15 µg/Lane) Lane 7: L6 cell lysate (15 µg/Lane) Lane 8: Mouse spleen tissue lysate (15 µg/Lane) Lane 9: Rat spleen tissue lysate (15 µg/Lane) Predicted band size: 29 kDa Observed band size: 34 kDa Exposure time: 11 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32725_mouse-pcna-mab-a6-g11-r-3920248485013.jpg)