| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89915-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52663-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47885-0.1mg | |||
| IHC-Prediluted | NBP2-48361 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92688SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-45204 | |||
| Azide and BSA Free | NBP2-34745-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44662 | |||
| Azide and BSA Free | NBP2-34737-0.1mg | |||
![Western Blot: PMP70 Antibody (PSH03-27) [NBP3-32834] - Western blot analysis of PMP70 on different lysates with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (20 ug/Lane) Lane 4: Caco-2 cell lysate (20 ug/Lane) Lane 5: SH-SY5Y cell lysate (20 ug/Lane) Lane 6: Human liver tissue lysate (40 ug/Lane) Lane 7: Mouse liver tissue lysate (40 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 76 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32834) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-288202412461658.jpg "Western Blot: PMP70 Antibody (PSH03-27) [NBP3-32834] - Western blot analysis of PMP70 on different lysates with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (20 ug/Lane) Lane 4: Caco-2 cell lysate (20 ug/Lane) Lane 5: SH-SY5Y cell lysate (20 ug/Lane) Lane 6: Human liver tissue lysate (40 ug/Lane) Lane 7: Mouse liver tissue lysate (40 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 76 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32834) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PMP70 Antibody (PSH03-27) [NBP3-32834] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32834) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-298202414333688.jpg)
![Immunocytochemistry/ Immunofluorescence: PMP70 Antibody (PSH03-27) [NBP3-32834] - Immunocytochemistry analysis of HepG2 cells labeling PMP70 with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-29202410334056.jpg)
![Flow Cytometry: PMP70 Antibody (PSH03-27) [NBP3-32834] - Flow cytometric analysis of NIH/3T3 cells labeling PMP70. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32834, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-2920241637212.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody (034) [NBP2-89915] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:35 kDa Observed band size:40 kDa](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-034-Western-Blot-NBP2-89915-img0003.jpg "Western Blot: Arginase 1/ARG1/liver Arginase Antibody (034) [NBP2-89915] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:35 kDa Observed band size:40 kDa")
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody (034) [NBP2-89915] - Staining of human ARG1 in human kidney with rabbit monoclonal antibody (1:1000).](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-034-Immunohistochemistry-Paraffin-NBP2-89915-img0002.jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody (034) [NBP2-89915] - Staining of human ARG1 in human liver with rabbit monoclonal antibody (1:1000).](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-034-Immunohistochemistry-Paraffin-NBP2-89915-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Arginase 1/ARG1/liver Arginase Antibody (034) [NBP2-89915] - Staining of ARG1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89915 antibody (dilution ratio 1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-89915_rabbit-monoclonal-arginase-1-arg1-liver-arginase-antibody-034-23520252135123.png)
![Flow Cytometry: Arginase 1/ARG1/liver Arginase Antibody (034) [NBP2-89915] - Analysis of Human ARG1 expression on HepG2 cells. The cells were treated, stained with NBP2-89915, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/nbp2-89915_rabbit-monoclonal-arginase-1-arg1-liver-arginase-antibody-034-235202511545610.png)
![Immunohistochemistry: E-Cadherin Antibody (SY0287) [NBP3-32290] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32290) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32290_rabbit-e-cadherin-mab-sy0287-29820241435227.jpg "Immunohistochemistry: E-Cadherin Antibody (SY0287) [NBP3-32290] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32290) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: E-Cadherin Antibody (SY0287) [NBP3-32290] - Immunofluorescence analysis of paraffin-embedded human colon tissue labeling E-Cadherin with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32290, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with 33258 (blue).](http://images.novusbio.com/fullsize/nbp3-32290_rabbit-e-cadherin-mab-sy0287-29820241435238.jpg)
![Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (SY0287) [NBP3-32290] - Immunocytochemistry analysis of HT-29 (positive) and MDA-MB-231 (negative) cells labeling E-Cadherin with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32290_rabbit-e-cadherin-mab-sy0287-29202410484863.jpg)
![Flow Cytometry: E-Cadherin Antibody (SY0287) [NBP3-32290] - Flow cytometric analysis of HT-29 (positive, red) and MDA-MB-231 (negative, green) cells labeling E-Cadherin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32290, red) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (HT-29 black, MDA-MB-231 light green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with an iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃.](http://images.novusbio.com/fullsize/nbp3-32290_rabbit-e-cadherin-mab-sy0287-29202416453231.jpg)
![Western Blot: E-Cadherin Antibody (SY0287) [NBP3-32290] - Western blot analysis of E-Cadherin on different lysates with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/5,000 dilution. Lane 1: MCF7 cell lysate Lane 2: MDA-MB-231 cell lysate (negative) Lane 3: HT-29 cell lysate Lane 4: HCT 116 cell lysate Lane 5: A431 cell lysate Lane 6: Caco-2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 97 kDa Observed band size: 80~120 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32290_rabbit-e-cadherin-mab-sy0287-3920248453539.jpg)
![Western Blot: TSC2 Antibody (SC05-59) [NBP2-67552] - Western blot analysis of TSC2 on MCF-7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab](http://images.novusbio.com/fullsize/TSC2-Antibody-SC05-59-Western-Blot-NBP2-67552-img0005.jpg "Western Blot: TSC2 Antibody (SC05-59) [NBP2-67552] - Western blot analysis of TSC2 on MCF-7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab")
![Immunohistochemistry-Paraffin: TSC2 Antibody (SC05-59) [NBP2-67552] - Analysis of paraffin-embedded mouse placenta tissue using anti-Tuberin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TSC2-Antibody-SC05-59-Immunohistochemistry-Paraffin-NBP2-67552-img0002.jpg)
![Flow Cytometry: TSC2 Antibody (SC05-59) [NBP2-67552] - Flow cytometric analysis of TSC2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/TSC2-Antibody-SC05-59-Flow-Cytometry-NBP2-67552-img0004.jpg)
![Western Blot: TSC2 Antibody (SC05-59) [NBP2-67552] - Analysis of Tuberin on Jurkat cell lysates using anti-Tuberin antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/TSC2-Antibody-SC05-59-Western-Blot-NBP2-67552-img0003.jpg)
![Flow Cytometry: TSC2 Antibody (SC05-59) [NBP2-67552] - Analysis of Hela cells with Tuberin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/TSC2-Antibody-SC05-59-Flow-Cytometry-NBP2-67552-img0001.jpg)
![Chromatin Immunoprecipitation: SOX2 Antibody (PO00-28) [NBP3-32972] - Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either SOX2 (NBP3-32972) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-26820241525104.jpg "Chromatin Immunoprecipitation: SOX2 Antibody (PO00-28) [NBP3-32972] - Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either SOX2 (NBP3-32972) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.")
![Western Blot: SOX2 Antibody (PO00-28) [NBP3-32972] - Western blot analysis of SOX2 on different lysates with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/2,000 dilution. Lane 1: NCCIT cell lysate Lane 2: F9 cell lysate Lane 3: HeLa cell lysate (negative) Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32972) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-28820241249344.jpg)
![Immunohistochemistry: SOX2 Antibody (PO00-28) [NBP3-32972] - Immunohistochemical analysis of paraffin-embedded human trachea tissue with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32972) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-298202414161844.jpg)
![Immunocytochemistry/ Immunofluorescence: SOX2 Antibody (PO00-28) [NBP3-32972] - Immunocytochemistry analysis of F9 cells labeling SOX2 with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-29202410334048.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody (4A8-2H3) [H00000059-M02] - Western blot analysis of alpha-Smooth Muscle Actin expression in human omentum, serous carcinoma, blotted with alpha-Smooth Muscle Actin Antibody (4A8-2H3). The band indicates an observed molecular weight of 42 kDa.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A8-2H3-Western-Blot-H00000059-M02-img0010.jpg "Western Blot: alpha-Smooth Muscle Actin Antibody (4A8-2H3) [H00000059-M02] - Western blot analysis of alpha-Smooth Muscle Actin expression in human omentum, serous carcinoma, blotted with alpha-Smooth Muscle Actin Antibody (4A8-2H3). The band indicates an observed molecular weight of 42 kDa.")
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (4A8-2H3) [H00000059-M02] - Immunofluorescence analysis of alpha-Smooth Muscle Actin on HeLa cell. Antibody concentration 1 - 10 ug/ml.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A8-2H3-Immunocytochemistry-Immunofluorescence-H00000059-M02-img0008.jpg)
![ELISA: alpha-Smooth Muscle Actin Antibody (4A8-2H3) [H00000059-M02] - Detection limit for recombinant GST tagged alpha-Smooth Muscle Actin Antibody is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A8-2H3-ELISA-H00000059-M02-img0011.jpg)
![Flow Cytometry: CD4 Antibody (MT310) - Chimeric [NBP2-52663] - Flow-cytometry using anti-CD4 antibody MT310 (NBP2-52663) Rhesus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of MT310 (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD4-Antibody-MT310-Chimeric-Flow-Cytometry-NBP2-52663-img0003.jpg "Flow Cytometry: CD4 Antibody (MT310) - Chimeric [NBP2-52663] - Flow-cytometry using anti-CD4 antibody MT310 (NBP2-52663) Rhesus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of MT310 (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
![Flow Cytometry: CD4 Antibody (MT310) - Chimeric [NBP2-52663] - Flow-cytometry using anti-CD4 antibody MT310 (NBP2-52663) Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of MT310 (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD4-Antibody-MT310-Chimeric-Flow-Cytometry-NBP2-52663-img0001.jpg)
![Flow Cytometry: CD4 Antibody (MT310) - Chimeric [NBP2-52663] - Flow-cytometry using anti-CD4 antibody MT310 (NBP2-52663) Cynomolgus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of MT310 (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD4-Antibody-MT310-Chimeric-Flow-Cytometry-NBP2-52663-img0002.jpg)
![Western Blot: Neuro2a Chloroquine Treated / Untreated Cell Lysate [NBP2-49688] - Mouse Neuoblast cells (Neuro2A) were treated with (+) and without (-) 50 uM Chloroquine overnight. Whole cell protein lysates were prepared in 1x Laemmli sample buffer and approximately 10 ug of each lysate (NBP2-49688) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml anti-LC3 (NB100-2220) and 1 ug/ml anti-alpha tubulin (NB100-690) as a loading control, and detected with the appropriate secondary antibodies using chemiluminescence.](http://images.novusbio.com/fullsize/Neuro2a-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49688-img0005.jpg "Western Blot: Neuro2a Chloroquine Treated / Untreated Cell Lysate [NBP2-49688] - Mouse Neuoblast cells (Neuro2A) were treated with (+) and without (-) 50 uM Chloroquine overnight. Whole cell protein lysates were prepared in 1x Laemmli sample buffer and approximately 10 ug of each lysate (NBP2-49688) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml anti-LC3 (NB100-2220) and 1 ug/ml anti-alpha tubulin (NB100-690) as a loading control, and detected with the appropriate secondary antibodies using chemiluminescence.")
![<b>Biological Strategies Validation. </b>Western Blot: Neuro2a Chloroquine Treated / Untreated Cell Lysate [NBP2-49688] - Western Blot: [NBP2-49688] - 10 ug of Chloroquine treated (+) and untreated (-) Neuro2A lysates in 1x Laemmli sample buffer (NBP2-49688) were separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml monoclonal anti-LC3 (NBP2--46892) and detected with an anti-rabbit secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Neuro2a-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49688-img0004.jpg)
![Western Blot: MUC1 Antibody (MUC1/967) [NBP2-44659] - Western Blot Analysis of human MCF-7 cell lysate using MUC1 antibody (MUC1/967).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-967-Western-Blot-NBP2-44659-img0004.jpg "Western Blot: MUC1 Antibody (MUC1/967) [NBP2-44659] - Western Blot Analysis of human MCF-7 cell lysate using MUC1 antibody (MUC1/967).")
![Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/967) [NBP2-44659] - Human Endometrial Carcinoma stained with EMA Monoclonal Antibody (MUC1/967).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-967-Immunohistochemistry-Paraffin-NBP2-44659-img0002.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/967) [NBP2-44659] - Human Breast Carcinoma stained with EMA Monoclonal Antibody (MUC1/967).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-967-Immunohistochemistry-Paraffin-NBP2-44659-img0001.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/967) [NBP2-44659] - Human Ovarian Carcinoma stained with EMA Monoclonal Antibody (MUC1/967).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-967-Immunohistochemistry-Paraffin-NBP2-44659-img0003.jpg)
![Western Blot: Vimentin Antibody (2A52) [NBP1-92688] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 1:10,000 of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP1-92688) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2A52-Knockout-Validated-NBP1-92688-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody (2A52) [NBP1-92688] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 1:10,000 of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP1-92688) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Vimentin Antibody (2A52) [NBP1-92688] - Immunocytochemistry/Immunofluorescence: [NBP1-92688] - View of mixed neuron/glial cultures stained with NBP1-92687 (green) and the GFAP rabbit polyclonal (NB300-141, red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the imate which appear green. Astrocytes may express primarily GFAP, or GFAP and Vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and Vimentin, the two proteins assemble to produce heteropolymer filaments.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2A52-Immunocytochemistry-Immunofluorescence-NBP1-92688-img0005.jpg)
![Western Blot: Vimentin Antibody (2A52) [NBP1-92688] - Analysis of cell and whole brain tissue lysates using mouse mAb to vimentin, NBP1-92688, dilution 1:5,000 in green: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS-1, [6] C6, and [7] rat brain. The band at about 50kDa mark corresponds to vimentin protein. The antibody does not recognize mouse vimentin (not shown).](http://images.novusbio.com/fullsize/Vimentin-Antibody-2A52-Western-Blot-NBP1-92688-img0006.jpg)
![Western Blot: Mitochondria Antibody (SPM198) [NBP2-32981] - Western Blot Analysis of HeLa cell lysate using Mitochondria Antibody (SPM198).](http://images.novusbio.com/fullsize/Mitochondria-Antibody-SPM198-Western-Blot-NBP2-32981-img0003.jpg "Western Blot: Mitochondria Antibody (SPM198) [NBP2-32981] - Western Blot Analysis of HeLa cell lysate using Mitochondria Antibody (SPM198).")
![Immunocytochemistry/Immunofluorescence: Mitochondria Antibody (SPM198) [NBP2-32981] - Confocal immunofluorescence image of HeLa cells using Mitochondria Antibody (SPM198) labeled in Green (CF488) and Red Dot is used to label the nuclei purple.](http://images.novusbio.com/fullsize/Mitochondria-Antibody-SPM198-Immunocytochemistry-Immunofluorescence-NBP2-32981-img0002.jpg)
![Immunohistochemistry-Paraffin: Mitochondria Antibody (SPM198) [NBP2-32981] - Formalin-fixed, paraffin-embedded human Pancreas stained with Mitochondria Monoclonal Antibody (SPM198).](http://images.novusbio.com/fullsize/Mitochondria-Antibody-SPM198-Immunohistochemistry-Paraffin-NBP2-32981-img0001.jpg)
![Western Blot: MUC1 Antibody (SPM132) [NBP2-32877] - Analysis of MDA-MB-231 cells transfected with/without an IL-8 silencer RNA (siIL-8) to evaluate the expression of MUC1 (NBP2-32877), VCAM-1, and ICAM-1. GAPDH load control was reused for illustrative purposes. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30105032/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MUC1-Antibody-SPM132-Western-Blot-NBP2-32877-img0003.jpg "Western Blot: MUC1 Antibody (SPM132) [NBP2-32877] - Analysis of MDA-MB-231 cells transfected with/without an IL-8 silencer RNA (siIL-8) to evaluate the expression of MUC1 (NBP2-32877), VCAM-1, and ICAM-1. GAPDH load control was reused for illustrative purposes. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30105032/) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: MUC1 Antibody (SPM132) [NBP2-32877] - Formalin-fixed, paraffin-embedded human Breast cancer stained with EMA Monoclonal Antibody (SPM132)](http://images.novusbio.com/fullsize/MUC1-Antibody-SPM132-Immunohistochemistry-Paraffin-NBP2-32877-img0001.jpg)
![Western Blot: MUC1 Antibody (SPM132) [NBP2-32877] - Analysis of MCF-7 cells treated +/- rhIL-8 at 10 ng/ml during 5 days to evaluate the expression of MUC1 (NBP2-32877), VCAM-1, and ICAM-1. GAPDH load control was reused for illustrative purposes. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30105032/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MUC1-Antibody-SPM132-Western-Blot-NBP2-32877-img0002.jpg)