| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1596SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86054-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26636-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48998-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77139-0.025mg | |||
![Western Blot: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) and hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Western-Blot-NB100-1596-img0005.jpg "Western Blot: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) and hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Aromatase Antibody [NB100-1596] - U2OS cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Aromatase Antibody NB100-1596 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1596-img0008.jpg)
![Immunohistochemistry-Paraffin: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control rabbits. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control ovaries. Nonlabeling was observed when the primary antibody was omitted. Bars: 200 um (1st image) and 20 um (2nd image). Granulose cells, gc; theca cells, tc. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Immunohistochemistry-Paraffin-NB100-1596-img0006.jpg)
![Western Blot: Aromatase Antibody [NB100-1596] - Detection of aromatase in human fetal temporal lobe lysate using NB 100-1596. ECL exposure, 1-2 minutes.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Western-Blot-NB100-1596-img0003.jpg)
![Immunohistochemistry-Paraffin: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of hypothyroid (Hypo; solid bar; n = 6) rabbits. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of hypothyroid ovaries. Nonlabeling was observed when the primary antibody was omitted. Bars: 100 um (1st image); and 20 um (2nd image). Granulose cells, gc; theca cells, tc. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Immunohistochemistry-Paraffin-NB100-1596-img0007.jpg)
![Simple Western: Aromatase Antibody [NB100-1596] - Aromatase expression by Western blotting (WES system) from in vitro activated M1 and M2 rat macrophages using Aromatase antibody. Controls include ovary (positive control) and ovary in which the primary antibody was preincubated with an aromatase blocking peptide for 1 h, as well as KGN cells with (positive control) and without (negative control) forskolin treatment. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29898754/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Aromatase Antibody-Simple Western-NB100-1596-img0009.jpg)
![Immunohistochemistry: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) & hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase (a) & Ponceau's Red stained membrane (b). Relative expression of aromatase in C & Hypo groups (c). Data are mean ± SEM. ∗∗P < 0.01. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control (d, e) & hypothyroid (f, g) ovaries. Nonlabeling was observed when the primary antibody was omitted (negative control; h). Bars: (d) 200 μm; (f) & (h) 100 μm; & (e) & (g) 20 μm. Granulose cells, gc; theca cells, tc. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28133606), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-31020241539251.jpg)
![Immunohistochemistry: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) & hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase (a) & Ponceau's Red stained membrane (b). Relative expression of aromatase in C & Hypo groups (c). Data are mean ± SEM. ∗∗P < 0.01. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control (d, e) & hypothyroid (f, g) ovaries. Nonlabeling was observed when the primary antibody was omitted (negative control; h). Bars: (d) 200 μm; (f) & (h) 100 μm; & (e) & (g) 20 μm. Granulose cells, gc; theca cells, tc. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28133606), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-310202415392577.jpg)
![Immunohistochemistry: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) & hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase (a) & Ponceau's Red stained membrane (b). Relative expression of aromatase in C & Hypo groups (c). Data are mean ± SEM. ∗∗P < 0.01. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control (d, e) & hypothyroid (f, g) ovaries. Nonlabeling was observed when the primary antibody was omitted (negative control; h). Bars: (d) 200 μm; (f) & (h) 100 μm; & (e) & (g) 20 μm. Granulose cells, gc; theca cells, tc. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28133606), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-310202415363733.jpg)
![Immunohistochemistry: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) & hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase (a) & Ponceau's Red stained membrane (b). Relative expression of aromatase in C & Hypo groups (c). Data are mean ± SEM. ∗∗P < 0.01. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control (d, e) & hypothyroid (f, g) ovaries. Nonlabeling was observed when the primary antibody was omitted (negative control; h). Bars: (d) 200 μm; (f) & (h) 100 μm; & (e) & (g) 20 μm. Granulose cells, gc; theca cells, tc. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28133606), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-310202415293319.jpg)
![Immunohistochemistry: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) & hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase (a) & Ponceau's Red stained membrane (b). Relative expression of aromatase in C & Hypo groups (c). Data are mean ± SEM. ∗∗P < 0.01. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control (d, e) & hypothyroid (f, g) ovaries. Nonlabeling was observed when the primary antibody was omitted (negative control; h). Bars: (d) 200 μm; (f) & (h) 100 μm; & (e) & (g) 20 μm. Granulose cells, gc; theca cells, tc. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28133606), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-310202415382447.jpg)
![Simple Western: Aromatase Antibody [NB100-1596] - Metformin decreases aromatase-positive, tumor-associated macrophages. a Quantification of aromatase-positive cells & b CD68-positive (CD68+) macrophages costaining for aromatase in the tumor border of tissues from control or metformin-treated rats. c Representative control & metformin-treated IHC images of CD68 & aromatase dual staining in tissue bordering mammary tumors (T) (arrows: yellow = CD68+aromatase+, brown = CD68+; scale bar = 50um). d Aromatase expression by Western blotting (WES system) from in vitro activated M1 & M2 rat macrophages using two different primary antibodies (Ab #1: Novus NB100-1596; Ab #2: clone 677, Baylor College of Medicine). Controls include ovary (positive control) & ovary in which the primary antibody was preincubated with an aromatase blocking peptide for 1 h, as well as KGN cells with (positive control) & without (negative control) forskolin treatment. e IHC intensity score for ER & PR (0 = no stain; 1 = weak; 2 = moderate; 3 = strong staining). *p < 0.05 Image collected & cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/s13058-018-0974-2), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-31020241682139.jpg)
![Immunohistochemistry-Paraffin: Tryptophanyl tRNA synthetase Antibody [NBP2-20743] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Tryptophanyl-tRNA-synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-20743-img0003.jpg "Immunohistochemistry-Paraffin: Tryptophanyl tRNA synthetase Antibody [NBP2-20743] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: Tryptophanyl tRNA synthetase Antibody [NBP2-20743] - Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Tryptophanyl-tRNA-synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-20743-img0002.jpg)
![Immunohistochemistry-Paraffin: Tryptophanyl tRNA synthetase Antibody [NBP2-20743] - Tryptophanyl tRNA synthetase antibody detects Tryptophanyl tRNA synthetase protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.Tryptophanyl tRNA synthetase stained by Tryptophanyl tRNA synthetase antibody (NBP2-20743) diluted at 1:85.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-20743_rabbit-polyclonal-tryptophanyl-trna-synthetase-antibody-9102024202192.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Tryptophanyl tRNA synthetase Antibody [NBP2-20743] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tryptophanyl tRNA synthetase antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-20743_rabbit-polyclonal-tryptophanyl-trna-synthetase-antibody-1610202419414595.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Tryptophanyl tRNA synthetase Antibody [NBP2-20743] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tryptophanyl tRNA synthetase antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-20743_rabbit-polyclonal-tryptophanyl-trna-synthetase-antibody-1610202419414510.jpg)
![Western Blot: Tryptophanyl tRNA synthetase Antibody [NBP2-20744] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Tryptophanyl-tRNA-synthetase-Antibody-Western-Blot-NBP2-20744-img0002.jpg "Western Blot: Tryptophanyl tRNA synthetase Antibody [NBP2-20744] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Tryptophanyl tRNA synthetase Antibody [NBP2-20744] - U87 xenograft, using Tryptophanyl tRNA synthetase antibody at 1:500 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Tryptophanyl-tRNA-synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-20744-img0003.jpg)
![Immunohistochemistry-Paraffin: Tryptophanyl tRNA synthetase Antibody [NBP2-20744] - Mouse prostate. Tryptophanyl tRNA synthetase antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Tryptophanyl-tRNA-synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-20744-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Tryptophanyl tRNA synthetase Antibody [NBP2-20744] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tryptophanyl tRNA synthetase antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-20744_rabbit-polyclonal-tryptophanyl-trna-synthetase-antibody-161020241941459.jpg)
![Western Blot: Aromatase Antibody (5C5N7) [NBP3-15438] - Western blot analysis of extracts of U-87MG cells, using Aromatase antibody (NBP3-15438) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Aromatase-Antibody-5C5N7-Western-Blot-NBP3-15438-img0003.jpg "Western Blot: Aromatase Antibody (5C5N7) [NBP3-15438] - Western blot analysis of extracts of U-87MG cells, using Aromatase antibody (NBP3-15438) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Immunohistochemistry: Aromatase Antibody (5C5N7) [NBP3-15438] - Immunofluorescence analysis of human placenta using Aromatase Rabbit mAb (NBP3-15438) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Aromatase-Antibody-5C5N7-Immunohistochemistry-NBP3-15438-img0002.jpg)
![Immunohistochemistry-Paraffin: Aromatase Antibody (5C5N7) [NBP3-15438] - Human colon using Aromatase Rabbit mAb (NBP3-15438) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Aromatase-Antibody-5C5N7-Immunohistochemistry-Paraffin-NBP3-15438-img0001.jpg)
![Immunohistochemistry: Aromatase Antibody (5C5N7) [Aromatase] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Aromatase Rabbit mAb at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15438_rabbit-aromatase-mab-5c5n7-52202517273588.jpg)
![Immunohistochemistry: Aromatase Antibody (5C5N7) [Aromatase] - Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using Aromatase Rabbit mAb at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15438_rabbit-aromatase-mab-5c5n7-5220251723570.jpg)
![Western Blot: Tryptophanyl tRNA synthetase Antibody [NBP2-32186] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-Tryptophanyl tRNA synthetase antibody NBP2-32186 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Tryptophanyl-tRNA-synthetase-Antibody-Western-Blot-NBP2-32186-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Tryptophanyl tRNA synthetase Antibody [NBP2-32186] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-Tryptophanyl tRNA synthetase antibody NBP2-32186 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Tryptophanyl tRNA synthetase Antibody [NBP2-32186] - Serial sections of human lung cancer. Antibody: Affinity purified rabbit anti- WARS used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/Tryptophanyl-tRNA-synthetase-Antibody-Immunohistochemistry-NBP2-32186-img0003.jpg)
![Immunoprecipitation: Tryptophanyl tRNA synthetase Antibody [NBP2-32186] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Tryptophanyl tRNA synthetase antibody NBP2-32186 used for IP at 6 ug per reaction. For blotting immunoprecipitated Tryptophanyl tRNA synthetase, NBP2-32186 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Tryptophanyl-tRNA-synthetase-Antibody-Immunoprecipitation-NBP2-32186-img0002.jpg)
![Immunohistochemistry: Aromatase Antibody (1H1) [NBP3-26636] - NBP3-26636 diluted at 1:300 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody](http://images.novusbio.com/fullsize/nbp3-26636_rabbit-aromatase-mab-1h1-2612025101148.jpg "Immunohistochemistry: Aromatase Antibody (1H1) [NBP3-26636] - NBP3-26636 diluted at 1:300 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody")
![Immunocytochemistry/Immunofluorescence: Aromatase Antibody (1H1) [NBP3-26636] - Staining of Hela cell with NBP3-26636 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26636_rabbit-aromatase-mab-1h1-2612025101147.jpg)
![Western Blot: Aromatase Antibody (1H1) [NBP3-26636] - Positive WB detected in: 293 whole cell lysate (20µg), K562 whole cell lysate (20µg), Hela whole cell lysate (20µg), MCF-7 whole cell lysate (20µg), PC-3 whole cell lysate (20µg)All lanes: CYP19A1 antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 58 kDaObserved band size: 58 kDaExposure time：120s](http://images.novusbio.com/fullsize/nbp3-26636_rabbit-aromatase-mab-1h1-2612025101143.jpg)
![Immunocytochemistry/Immunofluorescence: Aromatase Antibody (1H1) [NBP3-26636] - Staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26636_rabbit-aromatase-mab-1h1-26120251011416.jpg)
![Immunohistochemistry-Paraffin: Aromatase Antibody [NBP2-48998] - Staining in human placenta and prostate tissues using anti-CYP19A1 antibody. Corresponding CYP19A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Immunohistochemistry-Paraffin-NBP2-48998-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Aromatase Antibody [NBP2-48998] - Staining in human placenta and prostate tissues using anti-CYP19A1 antibody. Corresponding CYP19A1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Aromatase Antibody [NBP2-48998] - Staining of human cell line ASC TERT1 shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48998-img0008.jpg)
![Immunohistochemistry-Paraffin: Aromatase Antibody [NBP2-48998] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Immunohistochemistry-Paraffin-NBP2-48998-img0004.jpg)
![Immunohistochemistry-Paraffin: Aromatase Antibody [NBP2-48998] - Staining of human prostate shows low expression as expected.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Immunohistochemistry-Paraffin-NBP2-48998-img0005.jpg)
![Western Blot: Tryptophanyl tRNA synthetase Antibody [NBP2-32185] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-Tryptophanyl tRNA synthetase antibody NBP2-32185 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Tryptophanyl-tRNA-synthetase-Antibody-Western-Blot-NBP2-32185-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Tryptophanyl tRNA synthetase Antibody [NBP2-32185] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-Tryptophanyl tRNA synthetase antibody NBP2-32185 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: Tryptophanyl tRNA synthetase Antibody [NBP2-32185] - Samples: FFPE serial sections of human lung cancer. Antibody: Affinity purified rabbit anti- WARS used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/Tryptophanyl-tRNA-synthetase-Antibody-Immunohistochemistry-NBP2-32185-img0003.jpg)
![Immunoprecipitation: Tryptophanyl tRNA synthetase Antibody [NBP2-32185] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Tryptophanyl tRNA synthetase antibody NBP2-32185 used for IP at 6 ug per reaction. For blotting immunoprecipitated Tryptophanyl tRNA synthetase, NBP2-32185 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Tryptophanyl-tRNA-synthetase-Antibody-Immunoprecipitation-NBP2-32185-img0002.jpg)
![Western Blot: NDRG3 Antibody [NBP1-86046] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/NDRG3-Antibody-Western-Blot-NBP1-86046-img0005.jpg "Western Blot: NDRG3 Antibody [NBP1-86046] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue")
![Immunohistochemistry-Paraffin: NDRG3 Antibody [NBP1-86046] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NDRG3-Antibody-Immunohistochemistry-Paraffin-NBP1-86046-img0001.jpg)
![Western Blot: MAEL Antibody [NBP1-77139] - HeLa cell lysate with MAEL antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/MAEL-Antibody-Western-Blot-NBP1-77139-img0001.jpg "Western Blot: MAEL Antibody [NBP1-77139] - HeLa cell lysate with MAEL antibody at 1 ug/mL.")
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